LOCUS       NZ_KB905381            10180 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Sciscionella marina DSM 45152 A37UDRAFT_scaffold_1.2, whole genome
            shotgun sequence.
ACCESSION   NZ_KB905381
VERSION     NZ_KB905381.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Sciscionella marina DSM 45152
  ORGANISM  Sciscionella marina DSM 45152
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Sciscionella.
REFERENCE   1  (bases 1 to 910873)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Sciscionella marina DSM 45152
            collection_date                   :: SEP 2006
            lat_lon                           :: Missing
            depth                             :: 516 m
            alt_elev                          :: Missing
            country                           :: China
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 45152
            biotic_relationship               :: Free living
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: 19196758
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Sand; Sediment; Marine; Aquatic
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi11892
            Current Finishing Status          :: Level 2: High-Quality Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Isolation Site :: Marine sand sediment at a depth of about 500 m,
            the northern South China Sea
            Cell Shape                        :: Rod-shaped
            Temperature Range                 :: Mesophile
            Temperature Optimum               :: 28 C
            pH                                :: 7
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000379465.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 01:37:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,209
            CDSs (total)                      :: 8,146
            Genes (coding)                    :: 7,961
            CDSs (with protein)               :: 7,961
            Genes (RNA)                       :: 63
            rRNAs                             :: 4, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 1 of 185
            Pseudo Genes (frameshifted)       :: 52 of 185
            Pseudo Genes (incomplete)         :: 155 of 185
            Pseudo Genes (internal stop)      :: 15 of 185
            Pseudo Genes (multiple problems)  :: 34 of 185
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:59:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11631
            Orig. end                         :: 21811
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB905381.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405877
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos  Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-PGF and JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10180
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10180
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10180
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5180](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5180
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(531..1985)
                     /locus_tag="A37U_RS0105990"
     CDS             complement(531..1985)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946858.1"
                     /locus_tag="A37U_RS0105990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxylesterase family protein"
                     /protein_id="WP_020495708.1"
                     /transl_table=11
                     /translation="MPQHTHPVVETPAGAVRGIRDGSGERYRAIPYAAAPAGAGRFAPP
                     APHPGWSGVRDSTQPSPTAPQPARDFGRLDMIPYFGPAWVLGEEYLTVDVHTPAADGGR
                     RPVMVFVHGGGFVTGSSRAALYDGSAFARDDVVLVTVNYRLGIPGFLDLAGAPANRGLL
                     DVLAALSWVHDTIAVFGGDPANVTIFGQSAGATLVGALLATPQANGLFRRAIVQSGNGS
                     GAFTPEQARRVTAAAAAALGVEPTAEAFAPIPDERFLAILPALAGLDLHTSTATDPLAG
                     LSPFSLVLPVQPADDLADGPAGDVDLLIGTNTEEGHLYLAPSGDLESTTEADVLAVATR
                     VHAEPEAVLAAHRAARPDATPGELRSAVLGQALFEAGTARMARAHAQISGGRTHLYSFQ
                     YRSTALDGRLGAAHTVELPFVFDMADKPWLHGDTGLLGPAPAPTGLAAEVHGAWVAFAR
                     TGNPGWARYDPQRPVARILGGHPGAA"
     gene            complement(2008..2913)
                     /locus_tag="A37U_RS0105995"
     CDS             complement(2008..2913)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081847.1"
                     /locus_tag="A37U_RS0105995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_020495709.1"
                     /transl_table=11
                     /translation="MDQITLGDVTVTRVTEFYGSVEMPPGQFFPDSPDGSWDEHRDWLA
                     PDFLDAETDDCRTAIQSWLLRSEGRTILVDTGVGNHKERPYAPVWSRMDTHYLDNLAAA
                     GVRPEDVDIVINTHLHIDHVGWNTRLDGRSWVPTFPNATYLMPQRDFDFWDPANENDTV
                     LGRGNQNVFEDSVRPIHQAGLNHLWEGTHRIDKNLRLDLAPGHTPGSSVLTLESGGDRA
                     LFVGDLMHTALQIVEPDTNSCFCEDPVESRATRRKLLGRAAEDNALVFPAHFGGPGAAE
                     VERSGSKFVIKRWAGFSRVV"
     misc_feature    complement(2422..2424)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3042..3944
                     /locus_tag="A37U_RS0106000"
     CDS             3042..3944
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418614.1"
                     /locus_tag="A37U_RS0106000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_020495710.1"
                     /transl_table=11
                     /translation="MNRPGPLGDFLQARRTQLRPEDVGLRELGPRRRVAGLRREELAQL
                     AGVSVSYYARLEQGLSRGASAEVLDAIARALLLDEHEREHLDRLAGAARHVPRVRRPRP
                     EKLADETRDLLRAVDGVPAVVLGRRTDVLAWNTLGHALLAGHLDFLGPDDPARRPNMSR
                     MLFLDPHCRELYADWKRKVRAVVGNLRIAVGRHPEDPLLAELIGELTMRSPEFVALWGD
                     HRVAPCDAASYELHHPVVGAVTVTQQTLSIARSPEQVLIVCTTPADSSSEQALALLRQA
                     SSSQSSHLDGVDVAAKPLR"
     misc_feature    3870..3872
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4079..4612)
                     /locus_tag="A37U_RS0106005"
     CDS             complement(4079..4612)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /locus_tag="A37U_RS0106005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YetF domain-containing protein"
                     /protein_id="WP_205622881.1"
                     /transl_table=11
                     /translation="MLRTVLVYAGLALLLRLGGKRDLAQLNSFDLVVMLLLSNVVQNAV
                     IGNDNSLTGGLLGAAVLVALNAVLVRLVNRHPRLVRLFEGRSTSLIRDGRFLSKALHRL
                     GLRRADVVAACRRQGADDITEITDATLSPGGALIVTLDQEHRNASIGDVRRESDRLLTE
                     IRAELANLEHRLGR"
     misc_feature    complement(4295..4297)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5180
                     /locus_tag="A37U_RS0106015"
     CDS             5001..5180
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016349.4"
                     /locus_tag="A37U_RS0106015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="encapsulin"
                     /protein_id="WP_020495713.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.3e-10, bitscore:
                     35.7, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNHLPCELAPIPEAAWNALEEEATEQFTVHLAGRRLVDWPGSGWK
                     HSASGLGRKTRPRP"
     gene            complement(5635..6831)
                     /locus_tag="A37U_RS0106025"
     CDS             complement(5635..6831)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /locus_tag="A37U_RS0106025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_083933231.1"
                     /sec_met_domain="DAO (E-value: 3.6e-39, bitscore: 131.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRIVVIGAGMVGTAVAHALTTRGAEVVVLDRGEPGHGTSRTTFA
                     MDITARKTPRAYFDLSLQAAHRHTELAEQLTGDGRPSWLHHAPSIEIGVNEHDRTVMTE
                     RRDRLAEWGYPGEFVETSDLGELAAGLDLSRAADTTAVVYPGAAWYDPATFIARVFESI
                     HAELHPHTEVTALHRRGEHGWTVHTRTHTWEADTIVNCAGPDAARIAALAGTDLALEQV
                     PGIIGYTGPLPQPHTHAVLTLWDINLRPTPEQGLCLHSYPVDATLQGSATNGNTPPETL
                     KTDLAARAAEIVPALRHTPTTNLDIRIGIRPVPTDGLPLIGEHHDSPGLYTLATHSAIH
                     LAPILAEHAAGEILSQAPVPALEPYRPDRPTAPAMDESLNEMTRTYQTTNTTPTEVST"
     misc_feature    6853..6866
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.79"
                     /tool="antismash"
     gene            complement(6896..8233)
                     /locus_tag="A37U_RS42450"
     CDS             complement(6896..8233)
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /locus_tag="A37U_RS42450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MmgE/PrpD family protein"
                     /protein_id="WP_020495716.1"
                     /transl_table=11
                     /translation="MSHAFAEFILDSRWNAIPARIQHKALDCLLDVAGCTIAGSQTPAA
                     EQARSAASCLFGTGELTLFGTTETGSAPGVCLANGFAANAFDLDDGYRPLKGHPSALVF
                     PVVLAAAEESGCTGREFLSAYVAGVEVAIRAGHALHGYYDFYHGTGSWAPVGASAAATA
                     LLGGDTAAVARAAALAEFYAPLTPETRAVQTPSMVKDGIGWGAVTAFASARMALDGITA
                     PPSLLEKPETAESFDHEYGQRYRIEDLYFKQHPSCRWTHPAIDAALDAKTRTSHRPEDI
                     ASVTIETFDEATTLASITPATLEQAQFSLPWLVAIALVRDHIDIADTLPRHLEDPRDPT
                     INALASTVKLVRDETLDAAFPRTALARATLEFTDGTTSQSDIRAATGDPDNPLTAEQLH
                     AKFDRLTHPVLDQQGAAALSRCIATGPDLPDITALLRAATPSGTTT"
     gene            complement(8230..9618)
                     /locus_tag="A37U_RS0106035"
     CDS             complement(8230..9618)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /locus_tag="A37U_RS0106035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_020495717.1"
                     /transl_table=11
                     /translation="MTQEHPQDLVAIDDLPFSRFHKKLLVYSAAGPFCDGYVLGIIGPA
                     LAILSQPLGVTPVWSGLLGASVLIGIFVGGYLFGRITDLVGRRMMYTVDLVVFVVLSLL
                     QFIVTEPWQLFVLRLLLGVAVGADYPIASSMLAEFMPRKQRGPALASLIGAWWLGYVAA
                     YALGYVISVTGSSSWRWMLASSAVPALAAVLGRRNLPESPRWLMSRGRIAEARRVVSQH
                     LGTNVVLPDEDTGTPTTGSRRALTSPGYRLRTLFVAVFWMCQVMANYTISTFEPQILQA
                     MGIRNHMLFAAVISIFDLIGIIPAILLINRMGRRPLLNITFALSALPLLLLAIAADLPV
                     VWIVVLFIVFAVVNTAGSILQWVYPSEIFPTQVRASAVGFGTSVSRIGAAVGTFLLPMA
                     MADFGVSAATIGGAVVCLIGLVAGLVLAPETRGTTLAEAAASSAIPQPDHEIHATEKSQ
                     EQQP"
ORIGIN
        1 cgacctacgc tggcatcgac gccttgacga ggacccggcg tcacagtggc tgcgcaacca
       61 cgtacgcgcc gcgatcaaac cactcaccat tcgacttgcc acgctggacg gcaccacatc
      121 ccgagcgaca accggtgacc cggcacgctc cggtcggtag tggaccgcat cggaccatgc
      181 gctcggttta cgtggccgac gatggggcac cgtccgggaa ctcgtctgcg acgcgatcgc
      241 caaagccttg ttctccccgc caccggacga cgccggcgcg agcaggcaca tctgctggac
      301 acgcaggcat gaggctctgc gccccctctt cgagcgcgag gggtgagtgg tgcccgaacc
      361 cgggggacgc catcgaggta atcctcgccc cgacctactt tcgacacttc gcgagtggtc
      421 gaccgctgga cgaccacttc ctggagtcga tcgtcactgc agccctggcg ccatcgagga
      481 caaccagcgg atcgagcctg gccccggaca gtccacagtg gccgccccgc tcatgccgca
      541 ccggggtggc cgccaaggat ccgcgccacc ggtcgctgcg ggtcgtacct ggcccagccc
      601 gggttcccgg tcctggcgaa cgcgacccag gcgccgtgta cctcggccgc gagccctgtc
      661 ggggctgggg cggggccgag cagcccggtg tcaccgtgca accagggttt gtcagccatg
      721 tcgaacacga agggcagctc gacggtgtgg gcagcgccga gtcgcccgtc cagggccgtc
      781 gagcggtact ggaacgagta gaggtgcgtg cggccgcccg agatttgcgc gtgggcgcgc
      841 gccatgcgcg cagtgccggc ctcgaacagg gcctgcccga gcacggccga gcgcagctcg
      901 cccggtgtgg catccggccg tgccgcgcgg tgcgcggcga ggacggcctc cggttccgcg
      961 tgcacccggg tggcgacggc gagcacgtcg gcttccgtgg tggactccag gtcacccgac
     1021 ggcgcgagat agaggtgccc ctcttcggtg ttggtgccga tgagcaggtc gacatcgccg
     1081 gccgggccgt cggccagatc gtcggcgggc tggaccggca gaaccaggct gaacgggctg
     1141 agcccggcca gcgggtccgt ggcggtgctg gtgtgcaggt cgaggccggc cagtgcgggc
     1201 aggatcgcga ggaagcgctc gtccgggatc ggggcgaacg cctcggcggt cggttcgacg
     1261 ccaagggcgg cggccgccgc agcggtgacc cgtcgcgctt gctccggggt gaacgcgccg
     1321 ctgccgtttc cgctctgcac gatcgctcgc cggaacaggc cgttggcctg cggtgtggcg
     1381 agcagcgcgc cgacgagcgt tgcgcccgcg gactggccga agatcgtgac gttcgcgggg
     1441 tcgccgccga ataccgcgat cgtgtcgtgc acccagctga gtgcggcgag cacgtcgagc
     1501 agtccgcggt tggccggcgc acccgcgaga tcgaggaacc ccgggatgcc caggcggtag
     1561 ttcaccgtca cgaggacgac gtcgtcacgt gcgaacgcgc tgccgtcgta gagcgcggcg
     1621 cggctcgaac cggtgacgaa tccgccgccg tgcacgaaga ccatgacggg gcgcctgccg
     1681 ccgtctgcgg cgggcgtgtg gacatcgacg gtcaggtact cctcgccgag cacccaggcc
     1741 ggcccgaagt aagggatcat gtcgagccgg ccgaagtcgc gggccggttg gggagccgtt
     1801 ggcgagggct gcgtgctgtc acggacaccg gaccagccgg ggtgcggggc tggtggggcg
     1861 aaacggccgg cgccggccgg cgccgccgcg tacgggatgg cgcggtagcg ctcgccggat
     1921 ccgtcacgga taccgcgtac ggcgcccgcc ggtgtttcga caaccgggtg tgtgtgctgg
     1981 ggcatgggga ccgcttcctc tctgggatca gacgacgcgg gaaaagccag cccacctctt
     2041 gatcacgaat ttcgagccgc tgcgctcgac ttcagcggcg ccagggccgc cgaaatgcgc
     2101 cgggaacacg agcgcgttgt cctcggcggc acggccgagc agcttgcgcc gggtggcgcg
     2161 ggactcgacg gggtcctcgc agaagcagga gttggtatcc ggctcgacga tctgcagcgc
     2221 ggtgtgcatc agatccccga cgaacagggc ccgatcgccg ccggattcca gggtgagcac
     2281 ggacgagccg ggagtgtgcc cgggagcaag gtcgagccgc aggttcttgt cgatccggtg
     2341 cgtcccctcc cacaggtggt tcagccctgc ctggtggatc ggcctcacgc tgtcctcgaa
     2401 gacgttctgg ttcccccgcc ctaacacggt gtcgttctcg ttggccgggt cccagaaatc
     2461 gaagtcccgc tggggcatca ggtacgtggc gttcgggaac gtcggcaccc atgagcggcc
     2521 gtcgaggcgg gtgttccagc cgacgtggtc gatgtgcagg tgggtgttga tcacgatgtc
     2581 gacatcctcg ggccgcaccc cggcggcggc gaggttgtcg aggtaatgcg tgtccatgcg
     2641 gctccacacc ggcgcgtagg gtcgctcctt gtggttgcca acaccggtgt cgacgaggat
     2701 cgtgcgcccc tcactgcgca gcagccagga ttggatcgcc gtgcggcaat cgtcggtctc
     2761 cgcgtccaag aaatcaggtg ccagccagtc gcggtgttcg tcccacgagc cgtcggggct
     2821 gtccgggaag aactggcctg ggggcatctc gaccgagccg tagaactctg tgacacgggt
     2881 gacggtgacg tcgccgaggg tgatctgatc catgtggtcc gtcctcctgt tcggggtatg
     2941 ggaccgagcc tggacacacg ggaacgtggg gaccactccc gcgttgtcct accactggca
     3001 gggggtggcg gggcccttgc cgggcaccga atactggggt catgaacaga cctggcccac
     3061 tcggcgactt cctccaggcc cgacggaccc agttgcggcc cgaggacgtc ggcctgcgcg
     3121 aactcggccc ccgcaggcgg gtggccgggc tacggcgtga ggaactggcg cagttggcgg
     3181 gcgtcagtgt ctcgtactac gcacggctgg aacagggttt gtcccgcggt gcctcggccg
     3241 aggtgctcga cgcgatcgcc cgcgctctgc tgctcgacga gcacgagcgc gagcacctcg
     3301 accggctcgc cggcgccgcc cgccacgttc cccgagtgcg ccgtcctcgg cccgagaaac
     3361 tcgccgacga gacacgcgac ctgctccgcg ccgtcgacgg cgtcccggcg gtggtgctcg
     3421 gccgtcgtac cgatgtgctg gcgtggaaca cccttgggca cgccctgctg gccggtcatc
     3481 tggacttcct cggaccggat gacccggcgc ggcgcccgaa catgagccgg atgctgttcc
     3541 tcgacccgca ctgtcgggag ctgtacgcgg actggaagcg caaagtgcgc gcggtggtcg
     3601 gaaacctgcg catcgccgtc gggaggcacc cggaggaccc gctgctcgcg gagctgatcg
     3661 gcgagctgac gatgagaagc ccggagttcg tcgcactgtg gggagaccac cgcgtggcgc
     3721 catgcgatgc ggcgtcctac gagctgcacc atcccgtcgt cggggcggtg acggtgacgc
     3781 agcagacgct gtcgatcgcc cgttcaccgg agcaggtgct catcgtgtgc acaacgccgg
     3841 ccgactcctc ttcggagcag gccctcgcat tactccggca ggcgagcagc agccaatcgt
     3901 cgcacctgga cggcgtcgac gttgccgcga agccactgcg ataggtgtag tcggcccgga
     3961 tcatggtgac ggccgcgatc gagctatcgg ggagggtgtc gatacgtgcg tccgtttcga
     4021 tcgacactcc agctcgcggg cgtagctctt catcggtcga tccccgctgt gctgtggatc
     4081 agcggccgag tcgatgctcg aggttcgcga gttcggctcg gatttcggtc agcagtcggt
     4141 cgctttcccg gcgtacatcg ccgatggatg cgttgcggtg ctcctggtcg agggtgacga
     4201 tcagggcgcc gccgggagag agggtggcat cggtgatttc ggtgatgtcg tcggctccct
     4261 gacgacggca ggccgctacg acatcggcgc ggcgtaatcc gagccggtgc aatgctttcg
     4321 agaggaaacg gccgtcccgg atcaagctgg tcgaccgccc ttcgaacagt cgaaccaggc
     4381 gcggatgacg gttgacgagg cggaccagca ccgcgttgag ggcgacgagg acggccgcgc
     4441 cgagcaggcc gccggtgaga ctgttgtcat tgccgatgac cgcgttctgg acgacgttgg
     4501 acagcagcaa catgacgacc agatcgaagc tgttgagctg cgcgaggtca cgttttccgc
     4561 ccaagcgcag cagcagcgcc agtccggcgt agaccagtac ggtgcgcagc accttttccg
     4621 cggaggggat ttggaggttc cacaggtcgt gccacatgat cactcggctc ctttgcttgc
     4681 cattcggtca tgccgatcgc ccagcgtgtc gcaagactct accgtgagcg aggtttcggc
     4741 atgaccgacc acaacttcgc cagcacgaac ggaatcatca cgagtccgag tatcacgtgc
     4801 atctcttggg ccagccgaac accggcaggt acagcgaacc gaccacggcg ccaggttcgc
     4861 cgcgttcaca caggagcacc cggctgcttc cggattcgac acgggggata tcgcaccgga
     4921 cagatacctc gcttcggtcg aggccgaggg ggcgggcgat gacccgctcg gcctcggtcc
     4981 cctcgttggg cggtagccac atgaatcatc tgccgtgtga gctcgcgccg attcccgaag
     5041 cggcttggaa cgcactcgaa gaggaagcca cggagcagtt cacggtgcac ctggccggac
     5101 ggcgcctcgt cgactggccg ggttccggct ggaagcattc ggcctccgga ctgggtcgca
     5161 aaacgcgtcc tcgaccctga gcgacagaat caggggacgc caaccgcacc agcggaaggt
     5221 actcgcgctg ctgagcgtga tggaggcgtt ctggctccgg gattggtctc gtgacgaact
     5281 actggctgga cctggcgcct gcgatgttca ttctcgggct cgggttcgcc accggtgctc
     5341 gcgctgacgc agtccgcggt ctaccgggtc gctggggatc aagcggggta tcgcgtccgc
     5401 gctgctcgat tcggctcagc agatcggcgt cgcgctcgga ctcgctctgc ttgcggtggc
     5461 gcggcgacgg tggccgcggg tgtcctcgtc gtggccgctg tgatcgcgtt cggcacgctg
     5521 aacacccgcc cgagcagtgg gcatggcact gacgcgcgca ccggtaccga acgtgcagcg
     5581 agcagcggta ccggagaggc gaactgatcg atcagcggtc tgtctccggt ccggtcatgt
     5641 cgagacttcc gtgggtgtgg tgttggtggt ctggtaggtg cgggtcattt cgttgaggct
     5701 ttcgtccatc gcgggggcgg tgggccggtc gggccggtag ggctcgagcg cggggactgg
     5761 tgcctgggag aggatttcgc cggcggcgtg ttcggcgagg atcggggcga ggtggatggc
     5821 gctgtgtgtg gcgagggtgt agagaccggg actgtcatgg tgttcgccga tgagggggag
     5881 gccgtcggtg gggacgggcc tgatgccgat tcgtatgtcg aggttggtgg tgggcgtgtg
     5941 tcgtagtgcg ggcacgattt cggcggctcg tgccgcgagg tctgtcttga gtgtctcggg
     6001 tggggtgttg ccgtttgttg ctgacccctg aagggtcgcg tcgacgggat aggagtgcag
     6061 gcacaggccc tgttcggggg tggggcgcag gttgatgtcc cagagggtga gcacggcgtg
     6121 ggtgtgcggc tgggggagcg ggccggtgta gccgatgatt cccggaacct gctcgagtgc
     6181 gagatcggtt ccggcgagtg cggcgatgcg tgcggcgtcg gggccggcgc agttgacgat
     6241 cgtgtcggct tcccaggtgt gggtgcgggt gtgcacggtc cagccgtgtt caccgcgccg
     6301 gtggagtgcg gtgacttcgg tgtgggggtg aagctcggcg tggatggatt cgaacacgcg
     6361 cgcgatgaac gtggcggggt cataccaggc ggcgccgggg tagacgacgg ccgtggtgtc
     6421 cgcggcacgg gagaggtcga gtccggcggc gagttcaccg agatcgctgg tttctacgaa
     6481 ttctcccgga tagccccatt cggctagccg gtcgcgccgt tcggtcatga cggtccggtc
     6541 gtgttcgttg acgccgatct cgatcgaggg agcgtggtgc agccagcttg gccgcccatc
     6601 cccggtcagt tgttcggcta gttcggtgtg ccggtgtgct gcttggaggc tgagatcgaa
     6661 gtaggcacgg ggtgtctttc gggcggtgat gtccatcgcg aaggtggttc gcgaggtgcc
     6721 gtgtccgggt tcgccgcggt cgagtacgac gacctcggct ccccgggtgg tcagggcgtg
     6781 agcgacggcg gttccgacca ttccggctcc gataaccact attcgactca ttcggcaacc
     6841 tccatcgggg tttggacagt gtgcaagggg aagggcaacg aacagtgtgt tgcggtcagg
     6901 ttgttgttcc tgacggtgtg gccgcgcgca gcagggcagt gatgtccggt aggtccggcc
     6961 cggtagcgat acagcgcgag agcgccgccg ctccctgctg gtcgaggacg gggtgggtca
     7021 ggcggtcgaa tttggcgtgt agctgctcgg cggtgagggg gttgtccggg tcgccggtgg
     7081 cggcccggat atcggattgg cttgtggttc cgtcggtgaa ctcgagcgtg gcgcgggcca
     7141 gcgcggtccg ggggaacgcg gcgtcgagtg tctcgtcgcg gacgagtttc accgtggagg
     7201 cgagggcatt gatggtggga tcgcggggat cctcgagatg gcggggcagt gtgtccgcga
     7261 tgtcgatgtg atcgcgcacc agcgcgatcg cgaccagcca gggcaggctg aactgggctt
     7321 gttccaaggt ggccggggtt atcgaggcga gcgtggtcgc ctcgtcgaat gtttcgatgg
     7381 tgaccgaggc gatgtcttct ggccggtggc tggtccgggt cttggcatcg agtgcggcgt
     7441 cgatggccgg atgggtccag cgacagctcg ggtgttgttt gaagtagagg tcctcgatcc
     7501 ggtaacgctg gccgtactca tggtcgaacg actctgcggt ttccggtttt tccagcaggc
     7561 tcggtggcgc ggtgatcccg tcgagggcca tgcgtgctga ggcgaacgcg gtgaccgctc
     7621 cccacccgat cccgtccttg accatgctcg gtgtctgcac cgcgcgcgtt tctggtgtca
     7681 gcggcgcgta gaactcggcg agcgcggcag cacgcgcgac cgccgccgtg tcgcctccca
     7741 ggagcgctgt ggccgcggcg gacgcgccga ccggtgccca ggagccggta ccgtggtaga
     7801 agtcgtagta gccgtgcagg gcatgcccgg ctcggatcgc gacctcgact ccggcgacat
     7861 aggcggacag gaattcacgt cccgtgcaac cggactcttc ggcagcggcg agtaccacgg
     7921 ggaagaccag ggcgctcggg tgtcctttca acggccggta gccgtcatcg aggtcgaacg
     7981 cgttcgcggc gaacccgttg gccagacaga cacccggcgc actgccggtc tcagtcgtac
     8041 cgaacagggt cagctcgcca gtgccgaaca ggcaggacgc cgcgctgcgg gcctgctcgg
     8101 cggcaggggt ctggctgccg gcgatcgtgc agcccgcgac gtcgagcaga cagtccaggg
     8161 ccttgtgctg gattcgggca gggatcgcgt tccagcggga gtcgaggatg aattcggcga
     8221 aggcatggct catggctgct gctcctggga tttctcggtt gcgtggattt catggtcggg
     8281 ctgggggatg gcggaactgg cagccgcttc ggcgagggtc gttccacgag tctccggcgc
     8341 gaggaccaga ccggccacga gcccgatcag acagaccacg gcgccgccga tggtcgccgc
     8401 ggaaacaccg aagtcggcca tggccatggg caacagaaag gttcccaccg ccgcgccgat
     8461 cctgctgaca ctcgtgccga atcccaccgc cgaggcacgg acctgggtcg ggaagatctc
     8521 cgagggatac acccattgca ggatgctgcc ggcggtgttc accacggcga acacgatgaa
     8581 cagcacgaca atccagacca ccggcaggtc agcggcgatc gccaacaaca acagcggtag
     8641 cgcactcaag gcgaacgtga tgttcaacag cggccgtcgt cccatgcggt tgatcagcag
     8701 gatcgcggga atgatcccga tcaggtcgaa tatgctgatc actgccgcga acagcatatg
     8761 attccggatt cccatcgctt gcaggatctg cggctcgaag gtcgaaatcg tgtagttcgc
     8821 catcacctgg cacatccaga acaccgcgac gaacaaggtc cgcaaccggt atcccggtga
     8881 agtgagtgcc cgtcggcttc cggtcgtcgg tgtcccggtg tcctcgtcgg gcaacacgac
     8941 gttggtgccg aggtgctgac tgacaaccct tcgcgcttcg gcgatccggc ccctgctcat
     9001 cagccaacgt ggtgattctg gcaggttgcg acggcccaac accgcggcca gggcgggaac
     9061 cgcgctgctg gcgagcatcc agcgccacga cgaggaaccc gtcacgctga tgacataccc
     9121 cagggcataa gcagccacat aacccagcca ccacgccccg atcaggctcg ccagcgccgg
     9181 accacgctgt ttgcgcggca tgaactcggc cagcatcgaa cttgcgatcg gatagtcggc
     9241 accgaccgct acaccgagca gcagccgcaa cacgaacaac tgccacggct cggtgacgat
     9301 gaactgcagc agcgacagca ccacgaacac gacgaggtcg accgtgtaca tcatgcgacg
     9361 acccaccagg tcggtaatcc gaccgaacag gtagccaccg acgaagatcc cgatcagaac
     9421 actggcaccg agcagaccac tccacaccgg cgtcaccccg agcggttggc tcaagatcgc
     9481 cagagccgga ccgatgatgc ccagcacgta accgtcgcaa aacggcccag ccgccgagta
     9541 gaccagcagc ttcttatgga accgtgaaaa cggcaggtca tcgatcgcca ccaggtcctg
     9601 cgggtgctct tgcgtcatcg ccgcctcccg tctcatccgg aacgtcgctg gaaacctccg
     9661 ccgcacgcgc cggtgcgttc tccgcgactt tcacctcgag cctgcgttcc cgaccatcgt
     9721 gaccacccat ctattgattg ggaagcgctt tgacaatccg aatattcgct tgtttgataa
     9781 ggttcggtta tggatatcga cgttcgtcgt ctccacttgt tcatggccgt cgcggaagag
     9841 ctgcacttca gccgcgccgc taaacgcctg cacgtcagcc agcccctact gagccagcag
     9901 atccgtgctc tcgaacgtga actcggaacc cagctgttcg cgcgcagcag ccgggtcgtg
     9961 gagctcaccg cggccggccg agccttgatg gacgccaccc ctcgagtgct ctatgaactc
    10021 cggcgggcga ccgtcgacgt tcagcaggcg gcaagtggtg tgctcggacg gctcctggtc
    10081 ggatcggtac gcaccgcact ggccggtatc actcccagca tcatgcgcgc cttgcgcgcc
    10141 gagcatcccg agctccagat cgatctcgcc aacatggaaa
//
