LOCUS       NZ_KB891313            10381 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. ScaeMP-e10 B061DRAFT_scaffold_7.8, whole genome
            shotgun sequence.
ACCESSION   NZ_KB891313
VERSION     NZ_KB891313.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. ScaeMP-e10
  ORGANISM  Streptomyces sp. ScaeMP-e10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 379700)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Improved High-Quality Draft of Streptomyces sp. ScaeMP-e10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 379700)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 379700)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. ScaeMP-e10
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            Isolation Site                    :: Gulf of Mexico
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,185
            CDSs (total)                      :: 7,109
            Genes (coding)                    :: 6,996
            CDSs (with protein)               :: 6,996
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 1 (5S, 16S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 23 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 316329
            Orig. end                         :: 326710
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB891313.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405355
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10381
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="3"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10381
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10381
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5381](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5381)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(205..220)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.44"
                     /tool="antismash"
     gene            528..1370
                     /gene="griI"
                     /locus_tag="B061_RS0133010"
     CDS             528..1370
                     /EC_number="4.1.2.56"
                     /codon_start=1
                     /gene="griI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380462.1"
                     /locus_tag="B061_RS0133010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2-amino-4,
                     5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate synthase"
                     /protein_id="WP_018515802.1"
                     /transl_table=11
                     /translation="MAPNAPFARSLRLQRLHHHDPERLFIVPLDHSITDGPLTRAHHLD
                     ALVGQLASHHVDGIVLHKGSLRHVDPEWFTRTSLIVHLSASTVHAPDPNAKYLVSSVEE
                     SLRMGADAVSVHVNLGSDQERHQIADMAAVAEACDRWNVPLLAMMYPRGPKIDNPRDPA
                     LVAHAVQVAVDLGADLVKTLYVGSVEAMAEITASSPVPVVVVGGPRDSDEGRILAYVDE
                     ALRGGAAGVAMGRNVFQANDPGAMADKLSDLIHNSGHSRNSGNRSASPARAGATAGAA"
     gene            1442..2566
                     /gene="griH"
                     /locus_tag="B061_RS0133015"
     CDS             1442..2566
                     /EC_number="4.1.99.20"
                     /codon_start=1
                     /gene="griH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380461.1"
                     /locus_tag="B061_RS0133015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="3-amino-4-hydroxybenzoic acid synthase"
                     /protein_id="WP_018515803.1"
                     /transl_table=11
                     /translation="MSSSSSSKLTWLDIRSVGEARAAIVQEALHHKVEALVADDPAHLA
                     DLPPTVTKVLLVSGKQIPEEFGEATVVIVDPAKHGVTPAELAIKHPEIEFGRFVEIIDA
                     PTLEDACESSRTEKWSVLLFRDPTKIPLEIVIAAAARATGSMVTIAQDLEEAEILFGVL
                     EHGSDGVMMAPRTVGDAAELKRIAQAGIPNLNLTELRVMETSHIGMGERACVDTTTHFG
                     EDEGILVGSHSKGMILCVSETHPLPYMPTRPFRVNAGAIHSYTLGKDERTNYLSELKTG
                     SKLTAVDIKGNTRLVTVGRVKIETRPLISIDAEAPDGRRVNLILQDDWHVRVLGPGGTV
                     LNSTELKPGDTVLGYLPVEDRHVGYPINEFCLEK"
     gene            complement(2541..3944)
                     /locus_tag="B061_RS0133020"
     CDS             complement(2541..3944)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380460.1"
                     /locus_tag="B061_RS0133020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="WP_018515804.1"
                     /transl_table=11
                     /translation="MYVVLALCWLVVFFDGLDVTIYGAVMPYMLDDTAFGLDASAAGRI
                     GSWTTFGMLIGALTAGNITDWIGRRAVLVWCVLLFSAGSGVCALAGDPLQFGAGRFLAG
                     LGLGGLMPICLTVVAEFSPPRRMALATGALMTAYHAGGIAATGLGLGLAPDHGWRWPFA
                     AGVLPALIAVPLLLRFMPESPGVLYARGRFEQARTTAERYGLPAPTAADAPAAGAAGRL
                     RAVRELVAPGRALATLLLWAASFCGLLLVYGISTWLPQLMRSAGYGLSTSVSLLMVING
                     GGIVGMLVAGRVADRFGAVRVATCWFLLTAAGLALLATGPSLGLTYAVVAFTGIWLFSA
                     STMVYAVAGRFYPAADRAAGLGWVTGVGRLGAVVSPWLGGALVSRGLESSGFLVFAVGG
                     VLGAVAVVLVPAVAAARTRTGPGRAADEKELGGAAGAGEERGSRDSRGWQDSRDSRDSA
                     EPTSPGRTR"
     gene            complement(4037..4957)
                     /gene="griF"
                     /locus_tag="B061_RS0133025"
     CDS             complement(4037..4957)
                     /EC_number="1.10.3.15"
                     /codon_start=1
                     /gene="griF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380459.1"
                     /locus_tag="B061_RS0133025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="grixazone synthase"
                     /protein_id="WP_018515805.1"
                     /transl_table=11
                     /translation="MVHVRKNHLNMTAEEKRRFINAVLEIKRRGIYDRFVKLHIQVNST
                     DYLDKETGKRLGHVNPGFLPWHRQYLLKFEQALQKVDPRVTLPYWDWTTDHGENSPLWS
                     KDFMGGNGRPGDRRVMTGPFARRNGWKLNISVIPEGPEDPALNGNYTHDDRDYLVRDFG
                     TLTPDLPTPQELEQTLDLTVYDCPPWNHTSGGTPPYESFRNHLEGYTKFAWEPRLGKLH
                     GAAHVWTGGHMMYIGSPNDPVFFLNHCMIDRCWALWQARHPNVPHYLPTVPTQDVPDIN
                     TPLGPWHTKTPADLLDHTQFYTYDQ"
     misc_feature    4918..4932
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.34"
                     /tool="antismash"
     gene            complement(5001..5381)
                     /locus_tag="B061_RS0133030"
     CDS             complement(5001..5381)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380458.1"
                     /locus_tag="B061_RS0133030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /protein_id="WP_234017893.1"
                     /sec_met_domain="melC (E-value: 1.9e-33, bitscore: 109.9,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRREMVMATTGAALVAAAAAVPLLNGGDDTGAAAEAAAPAKAAG
                     QEHTERYLGRTIRVATAADGGGVYIDGRPLHLMKFADDAYLSSMCHYEMAPTPLDAARR
                     AVEELRGAALQPSTHGTHVTHL"
     misc_feature    complement(5377..5390)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.62"
                     /tool="antismash"
     gene            complement(5448..6818)
                     /locus_tag="B061_RS0133035"
     CDS             complement(5448..6818)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380457.1"
                     /locus_tag="B061_RS0133035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_018515807.1"
                     /sec_met_domain="Aldedh (E-value: 1.1e-12, bitscore: 41.6,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAAPATVVLDPSVCGRRVASSDRSRLPSVLGGDLADIGTAPRL
                     LAVAALNEIRAHADGRPPAFEVFTGAAELFATATLDGESPQEYARRVCHATGLTATAVE
                     QAVADLTAELRALPATTAAELPATGFGDGFDTRWVPRGRVFAAVMASNHPVPNISWAQA
                     LFHGYSVLVKPGSRDPFTPARLLAALTAAGLEPSRAAFLPCSREVGEYLLREADRGIIY
                     GGESAVSTWHQRESVAVRGPGRTKALLDTAADDAVIEHLALSASFDGGTRCTNLSAVLT
                     TGAVEDVADRLAERLAKLPSLPATDGEATLLVVNRARAEALREQVAALRASLTDHSARA
                     GEYETVVELIDGSFLLRPVVLSADRAGHPAVGTELPFPFVVVAPWTEADGVEPLRDSLV
                     LNLLTDREELVEAAVREPSVRKVTRGRVLPWTATPGIPHDDNYTQFLLEPKGLVAQR"
     gene            complement(6818..7876)
                     /locus_tag="B061_RS0133040"
     CDS             complement(6818..7876)
                     /NRPS_PKS="Domain: AMP-binding (78-301). E-value: 9.4e-13.
                     Score: 39.4. Matches aSDomain:
                     nrpspksdomains_B061_RS0133040_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380456.1"
                     /locus_tag="B061_RS0133040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_026242173.1"
                     /sec_met_domain="AMP-binding (E-value: 8.3e-09, bitscore:
                     28.8, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHIIDPEFDSHIRKLMAWHFGAETGSPFWLGKYDGLGFDPVAEV
                     QGLADLTRFPDVSAELRSVPAQDLIPRGLSDRPFRVYDSGGTTGSPKRIVDSAYRSQVT
                     EWARERLIANGVPADGNWLHLGPSGPHVIGFDTGRYAALGGGIFYTVDLDPRWVKKLLS
                     DGRGEEADAYIQHLLDQAETILESQDVAVLNTTPPLLEAICARPRLYELVRTKVRAIIW
                     AGTSISRESLKQLDEVFFPAAHVVGVYGNSLMGVAPQRPARPGDAYPSVFEPFPDTTRL
                     HLVDEAGEPVAYGERGRVRLHLVSEEMFLPNVLERDTAIRIEPQDGSTVDGLADVQTYR
                     TLDDVAIIEGVY"
     aSDomain        complement(6974..7642)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B061_RS0133040_AMP-binding.1"
                     /evalue="9.40E-13"
                     /label="B061_RS0133040_AMP-binding.1"
                     /locus_tag="B061_RS0133040"
                     /protein_end="301"
                     /protein_start="78"
                     /score="39.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FRVYDSGGTTGSPKRIVDSAYRSQVTEWARERLIANGVPADGNWL
                     HLGPSGPHVIGFDTGRYAALGGGIFYTVDLDPRWVKKLLSDGRGEEADAYIQHLLDQAE
                     TILESQDVAVLNTTPPLLEAICARPRLYELVRTKVRAIIWAGTSISRESLKQLDEVFFP
                     AAHVVGVYGNSLMGVAPQRPARPGDAYPSVFEPFPDTTRLHLVDEAGEPVAYGERGRVR
                     L"
     gene            complement(8088..9440)
                     /locus_tag="B061_RS0133045"
     CDS             complement(8088..9440)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968442.1"
                     /locus_tag="B061_RS0133045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_018515809.1"
                     /transl_table=11
                     /translation="MTTHEAASTAAVSTASSPGARSDGLRYGALFGPVVFGVTAAGVAL
                     PDVASSLDASATATAWVLTAHALALGVGTALFGRLADTRGVRAALLLGSLALAVGAVIC
                     LVAPNLGVLVAGRFVQAAGSGAMAACAMALTASVPPERRAQVFAGFGATMAIFSASATL
                     AGGVFTEWVTWRIALVLPALSLLVVPLCLAGAPARKNSGRSMDLPGAALLTLTAMSFLV
                     LIQSSALALSTPLVTATAVVLLLSGAGLIWRTRSSETSFVPRALVTDGVFLKAAVIGIG
                     VYGGLFAAMYAVPQLLVRDHGWSVLAVGVWLLPGAVVGAILSRLAGRLTAGDHGNLLLG
                     AVSLAFAAALAGALAGSGVVLLILGASLGFSAFSVTQVFTTALMSGHIEPARRGGAMGL
                     LNLTFFVGGGVGSATAGALAKSMSLSTALGVVALFPLIAALVAFTLSRRTA"
     misc_feature    9907..9924
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.59"
                     /tool="antismash"
ORIGIN
        1 tccgtaccca cgacaccccg gacccctcac ccgacgtctt ccggttcctg gccgaggagt
       61 cgatcccggc cgacatgacc acggtcgcct ccgcgcccgg cggcggcttc tcgctcggcc
      121 tcacggtcca ccgttcgcac gcggcggacg tcagcaggtc cttcagcgac ctcatggcgc
      181 cgcgccggca cggggtggag gtcgaggacc aggtcgccaa ggtctcgatc gtcggccagg
      241 gcctgctcag ccggcccgag tacgcctccc ggatgctggc ctcgctcgtc ggctccggca
      301 tcagcgcccg gtcgctgtcc gcgtcccagc tgcgcgtctc ggtgaccgtg ccgagctccg
      361 acgcggtccg cgcggtcggc ctgctgcaca gcgagttcgg cctcggcgcc gaggccggcg
      421 cgatgcccgc aagcctctcc cctcggccct gaccccggcc ctggccccga ccccggtcct
      481 gatcccggcc cgaggcccgc cccgccgaca ccagacaagg agcaccgatg gccccgaacg
      541 cccccttcgc caggagtctg cgactccagc ggctccacca ccacgacccc gagcggctgt
      601 tcatcgtccc gctcgaccac tcgatcaccg acggcccgct gacccgcgcg caccacctgg
      661 acgcgctcgt cggccagctg gcctcgcacc acgtcgacgg catcgtcctg cacaagggat
      721 cgctgcggca cgtggacccg gagtggttca cccggacctc gctgatcgtg cacctgagcg
      781 ccagcacggt ccacgccccc gaccccaacg ccaagtacct ggtgtcgagc gtcgaggaga
      841 gcctgcggat gggcgcggac gcggtgagcg tccacgtcaa tctcggctcc gaccaggagc
      901 gtcaccagat cgcggacatg gcggcggtcg cggaggcctg cgaccgctgg aacgtaccgc
      961 tgctggcgat gatgtacccg cgcggcccca agatcgacaa tccgcgcgat ccggcgctcg
     1021 tcgcccacgc cgtccaggtg gccgtcgacc tcggcgccga cctggtcaag acgctgtacg
     1081 tcggatcggt cgaggcgatg gccgagatca ccgcgtcctc gccggttccg gtcgtcgtgg
     1141 tcggcggacc gcgcgacagc gacgagggcc ggatcctcgc ctatgtcgac gaggcgctgc
     1201 gcggcggcgc ggccggtgtc gccatgggcc gcaacgtctt ccaggccaac gatcccgggg
     1261 cgatggcgga caagctctcc gacctcatcc acaacagcgg tcacagccgc aacagcggca
     1321 acaggtccgc ttcgccggcg cgcgccggcg cgacagccgg cgccgcctga tccgtcccgc
     1381 cgaacgatct caactcccct gttccatcac gtcagtccat cgcttctgag gagttcttcg
     1441 catgtcttcg tcttcgtctt cgaaactgac ctggctcgac atccgttccg tcggtgaggc
     1501 ccgtgccgcc atcgtccagg aggccctgca ccacaaggtc gaggccctgg tcgccgacga
     1561 tcccgcccac ctcgcggacc tgccgccgac cgtgaccaag gtcctgctgg tgtccggcaa
     1621 gcagatcccg gaggagttcg gcgaggccac cgtcgtcatc gtcgacccgg ccaagcacgg
     1681 ggtgaccccg gccgaactgg ctatcaagca cccggagatc gagttcggcc ggttcgtgga
     1741 gatcatcgac gcgccgacgc tggaggacgc ctgcgagtcc tcgcgtaccg agaagtggtc
     1801 cgtcctgctg ttccgcgacc cgaccaagat cccgctggag atcgtgatcg ccgccgccgc
     1861 gcgcgccacc ggctcgatgg tgaccatcgc gcaggacctg gaggaggcgg agatcctctt
     1921 cggcgtgctg gagcacggtt cggacggcgt gatgatggcg ccgcggaccg tcggcgacgc
     1981 cgccgagctc aagcggatcg cccaggccgg catccccaac ctcaacctca ccgagctgcg
     2041 cgtgatggag accagccaca tcggcatggg tgagcgggcc tgcgtggaca ccaccacgca
     2101 cttcggcgag gacgagggca tcctggtcgg ctcgcactcc aagggcatga tcctgtgcgt
     2161 cagcgagacg cacccgctgc cgtacatgcc gacccggccg ttccgcgtca acgccggtgc
     2221 catccactcg tacacactcg gcaaggacga gcgcaccaac tacctcagcg agctgaagac
     2281 cggcagcaag ctgaccgccg tggacatcaa gggcaacacg cggctggtca cggtgggccg
     2341 cgtgaagatc gagacgcgtc cgctgatctc catcgacgcc gaggccccgg acggccggcg
     2401 cgtcaacctg atcctccagg acgactggca cgtccgggtc ctcggccccg gtggcacggt
     2461 cctcaacagc accgagctga agccgggcga cacggtcctc ggctacctgc ccgtcgagga
     2521 ccgtcacgtc ggctacccga tcaacgagtt ctgcctggag aagtaggctc cgccgagtcg
     2581 cgggagtccc gcgagtcctg ccaacctcgc gagtcccgcg aaccccgctc ctccccggcc
     2641 ccggccgcgc cgcccagctc cttctcatcg gcggcgcgtc cggggccggt gcgtgtgcgg
     2701 gccgcggcga cggccggcac caggaccacg gccacggctc cgaggactcc gccgaccgcg
     2761 aagaccagga agccgctgct ctccagcccc cggctgacca gcgcaccgcc gagccacggg
     2821 ctgacgaccg cgccgagccg gccgacgccg gtgacccagc ccaggcccgc ggcgcggtcg
     2881 gccgccgggt agaagcggcc ggcgaccgcg tagaccatgg tgctggcgct gaacagccag
     2941 atgccggtga aggcgacgac cgcgtacgtg aggccgagcg agggtccggt ggccagcagc
     3001 gccagtccgg ccgccgtcag caggaaccag caggtggcga cgcggaccgc gccgaaccgg
     3061 tcggcgacgc ggccggcgac cagcatgccg acgatgccgc cgccgttgat gaccatgagc
     3121 aggctgaccg aggtggacag gccgtaaccg gcggagcgca tcagctgcgg cagccaggtg
     3181 gagatgccgt acacgagcag cagtccgcag aaggaggcgg cccacagcag cagtgtcgcc
     3241 aacgcccgcc caggtgccac gagttcgcgt acggcacgca gtcgtccggc agccccggcg
     3301 gccggtgcgt cggcggcggt cggggccgga agtccgtacc gttcggcggt cgtccgcgcc
     3361 tgctcgaacc ggccgcgcgc gtagagcacg ccgggcgact ccggcatgaa gcgcaggagc
     3421 agtggtacgg cgatgagcgc gggcagcacg cccgcggcga acggccaccg ccacccgtgg
     3481 tccggggcga gccccagacc gaggcccgtc gccgcgatac cgccggcgtg gtaggcggtc
     3541 atgagggcgc cggtggccag cgccatccgg cgcggcggcg agaactccgc cacgacggtc
     3601 aggcagatcg gcatcaggcc gccgaggccg agaccggcca ggaagcggcc ggcgccgaac
     3661 tggagcgggt ctccggccag cgcgcagacc ccggatccgg cggagaacag cagcacgcac
     3721 cagaccagca cggcgcgtct gccgatccag tcggtgatgt tgcccgccgt cagcgcgccg
     3781 atgagcatgc cgaaggtggt ccagctgccg attctgccgg ccgcggaggc gtcgaggccg
     3841 aaggcggtgt cgtcgagcat gtacggcatc acggcgccgt agatcgtgac gtccaggccg
     3901 tcgaagaaga cgacgagcca gcacagagcg agtaccacgt acacggtgtc tctcctggtt
     3961 cgagggggca ttggggtccg cacaacgcgg cggcgccgga caacggggat ccgctgtccg
     4021 gcgccgccgc gtcggtctac tggtcgtagg tgtagaactg cgtgtggtcg agcaggtccg
     4081 ccggggtctt ggtgtgccac ggcccgagcg gggtgttgat gtccggcacg tcctgggtcg
     4141 gcacggtcgg caggtagtgc ggcacgttcg ggtggcgcgc ctgccacagg gcccagcagc
     4201 ggtcgatcat gcagtggttg aggaagaaca ccgggtcgtt cggcgagccg atgtacatca
     4261 tgtggccgcc ggtccacacg tgcgcggcgc cgtgcagctt gccgagccgg ggctcccagg
     4321 cgaacttggt gtagccctcc aggtggttgc ggaagctctc gtacgggggt gtgccgccgg
     4381 aggtgtggtt ccacggcggg cagtcgtaga ccgtgaggtc gagcgtctgc tccagctcct
     4441 gcggcgtcgg caggtccggg gtgagcgtgc cgaagtcccg tacgaggtag tcgcggtcgt
     4501 cgtgggtgta gttgccgttc agcgccggat cctccgggcc ttccgggatg acgctgatgt
     4561 tcagcttcca gccgttgcgg cgcgcgaacg gtccggtcat gacgcggcgg tcgccgggcc
     4621 ggccgttgcc gcccatgaag tccttggacc acagcggcga gttctcgccg tggtcggtgg
     4681 tccagtccca gtacggcagc gtgacgcgcg gatcgacctt ctggagggcc tgttcgaact
     4741 tcaggaggta ctgccggtgc cacggcagga agcccgggtt gacgtggccg aggcgcttgc
     4801 cggtctcctt gtcgaggtag tcggtggagt tgacctgtat atggagcttg acgaaccggt
     4861 cgtagatgcc gcgtctcttg atctccagga ccgcgttgat gaagcgccgc ttctcctcgg
     4921 ccgtcatgtt caggtggttc ttgcgtacgt ggaccatcgc tgggattcct tcttcagggg
     4981 ggccaggggt gccagaggct tcagaggtgc gtgacgtgcg tgccgtgcgt ggagggctgg
     5041 agggcggcgc cgcgcagctc ctcgacggcg cggcgggcgg cgtccagcgg ggtgggggcc
     5101 atctcgtagt ggcacatgga gctgaggtag gcgtcgtcgg cgaacttcat gaggtgcagc
     5161 gggcggccgt cgatgtagac gccgccgccg tccgccgcgg tggccacgcg tatggtgcgg
     5221 ccgaggtagc gctcggtgtg ttcctggccc gcggccttcg ccggtgcggc ggcctcggcg
     5281 gcggctccgg tgtcgtcgcc gccgttcagc agcggtacgg cggccgcggc ggcgacgagc
     5341 gccgctccgg tggtggccat gaccatttct cgcctgttca tgggcatgcg aaatccctct
     5401 ctcgcggtct ggtgtgtctt cggtggatgg gccggggctc ggggacgtca gcgctgggcc
     5461 acgaggccct tcggctccag gaggaactgc gtgtagttgt cgtcgtgcgg gatgcccggt
     5521 gtcgcggtcc acggcaggac ccggccgcgc gtcaccttcc gtacgctcgg ctcccgtacc
     5581 gcggcctcca ccagctcctc gcggtcggtg agcaggttga ggacgagcga gtcgcggagc
     5641 ggctccacgc cgtcggcctc cgtccacggg gccaccacga cgaacgggaa cgggagttcc
     5701 gtgccgaccg ccggatggcc ggcgcggtcg gcggagagca cgaccggccg cagcaggaac
     5761 gagccgtcga tcagctccac gacggtctcg tactcgcccg cgcgtgcgct gtggtcggtg
     5821 agcgaggcgc ggagggcggc gacctgttcg cgcagcgcct cggcgcgggc ccggttcacc
     5881 accaggagcg tcgcctctcc gtcggtcgcc gggagggacg gcagcttcgc gagccgctcc
     5941 gcgagccggt cggcgacgtc ctcgaccgcc ccggtggtga ggaccgccga gaggttggtg
     6001 caccgggtgc cgccgtcgaa ggaggcggag agcgccaggt gctcgatgac ggcgtcgtcc
     6061 gcagccgtgt ccaggagcgc cttggtccgg ccggggccgc gtacggccac cgattcgcgc
     6121 tggtgccacg tggagacggc gctctcaccg ccgtagatga tgccgcggtc ggcctcccgc
     6181 agcaggtact cgccgacctc gcgcgagcac ggcaggaacg cggccctgct cggctccagg
     6241 ccggccgccg tcagcgcggc cagcagcctg gccggggtga acgggtcgcg gctgcccggc
     6301 ttgacgagca cgctgtagcc gtggaacagc gcttgggccc aggagatgtt cggcaccggg
     6361 tggttgctcg ccatcacggc ggcgaagacc cggccgcgcg gtacccagcg ggtgtcgaag
     6421 ccgtcgccga agccggtggc gggcagttcg gcggcggtgg tcgccgggag cgcgcgcagt
     6481 tcggcggtca ggtcggcgac ggcctgttcg acggcggtgg cggtgaggcc ggtggcgtgg
     6541 cagacgcgcc gcgcgtactc ctgcgggctc tcgccgtcca gcgtcgcggt ggcgaagagt
     6601 tcggccgccc cggtgaacac ctcgaaggcg ggcggtctgc catccgcgtg cgcgcggatc
     6661 tcgttgagcg cggcgaccgc gaggagccgg ggcgcggtcc cgatgtcggc gaggtcgccg
     6721 ccgagcacgc tcggcagccg ggagcggtcg gagctggcga cgcggcggcc gcagacgctc
     6781 gggtcgagga cgacggtggc cggtgcggcg gtgctcatca gtagacccct tcgatgatgg
     6841 ccacgtcgtc gagggtgcgg taggtctgca catcggcgag gccgtccacg gtggacccgt
     6901 cctgcggctc gatccggatc gcggtgtccc gctccaggac gttcggcaag aacatctcct
     6961 ccgagaccag gtggagccgg acccggccgc gttcgccgta cgccaccggc tcaccggcct
     7021 cgtcgacgag gtggaggcgc gtggtgtccg ggaacggctc gaagacgctc gggtaggcgt
     7081 cgccgggccg ggcgggccgc tgcggcgcca cgcccatcag gctgttgccg tagacgccga
     7141 cgacgtgcgc cgccgggaag aagacctcgt cgagctgctt gaggctctcc cggctgatgg
     7201 acgttccggc ccagatgatc gcgcggacct tggtgcgcac cagctcgtac aggcgcggcc
     7261 gggcgcagat cgcctccagg agcggcggcg tggtgttgag caccgcgacg tcctggctct
     7321 ccaggatggt ctccgcctgg tcgagcaggt gctgaatgta ggcgtcggcc tcctcgccgc
     7381 ggccgtccga caggagcttc ttcacccagc gcgggtcgag gtcgacggtg tagaagatgc
     7441 cgccgccgag tgcggcgtaa cggcccgtgt cgaagcctat gacgtgcggg ccgctcggtc
     7501 ccaggtggag ccagttgccg tccgccggga cgccgttcgc gatgaggcgt tcccgcgccc
     7561 actcggtcac ctgcgaccgg taggcgctgt ccacgatgcg cttgggcgat ccggtggtgc
     7621 cgccggagtc gtagacgcgg aacgggcggt cggacaggcc gcgcgggatc aggtcctgcg
     7681 ccggtacgga gcggagttcg gcgctgacgt ccgggaagcg tgtcaggtcg gccaggccct
     7741 gcacctcggc gaccgggtcg aagccgagcc cgtcgtactt gccgagccag aacggcgagc
     7801 cggtctcggc accgaagtgc caggccatca acttacgtat gtggctgtcg aattcaggat
     7861 caatgatgtg gctcatgacg gtcttccgct ctcctgatca cggggcaagg gcggtacggg
     7921 gtccatcgtg ctggcgaggc ctatcggtga cgtatcacct gcgtgtcgtg gaacgcggcc
     7981 gcccgcacag caccgttcgg gcgggctgta cgggcggtgc tgtacgggcg gttgtgcgcg
     8041 tgcggttgtg cgcgtgcggt tctgtacggg cttcgcggcg ggcggcgcta tgccgtgcgc
     8101 cgggacagcg tgaaggcgac cagtgccgcg atcagcggga agagcgcgac gacgccgagc
     8161 gccgtggaga gcgacatcga cttcgccagc gccccggcgg tggcgctgcc gacaccgccg
     8221 cccacgaaga acgtgaggtt gagcagcccc atcgcaccac cgcgccgggc cggttcgatg
     8281 tgtccggaca tcagggcggt ggtgaacacc tgggtgacgg agaaggcgga gaagccgagc
     8341 gaggcaccga ggatcaggag cacgacgccg gagccggcca gcgcgccggc gagcgccgcc
     8401 gcgaaggcga gggagaccgc gccgagcagc aggttgccgt ggtcgcccgc ggtgagccgg
     8461 ccggccagcc gggagaggat cgcgccgacg accgctccgg gcaggagcca gacgccgacg
     8521 gccaggacgc tccagccgtg gtcccggacg aggagctgcg gtacggcgta catcgcggcg
     8581 aacagaccgc cgtacacacc gatgccgatg acggcggcct tcaggaagac tccgtcggtg
     8641 acgagcgcgc gcggcacgaa ggaggtctcg ctgctgcgcg tacgccagat cagtccggca
     8701 cccgacagga gcagcacgac ggcggtcgcg gtcaccagcg gggtggacag cgcgagcgcg
     8761 gaggactgga tcagcacgag gaacgacatg gcggtgagcg tcagcagggc cgctcccggc
     8821 aggtccatcg accggccgga gttcttccgc gccggcgctc cggccaggca cagcggcacg
     8881 acgaggaggg agagcgccgg caggaccagg gcgatgcgcc aggtcaccca ctcggtgaag
     8941 acgccgccgg ccagggtggc gctggccgag aagatcgcca tcgtggcacc gaatccggcg
     9001 aagacctgcg cccgacgttc cggcggtacg gaggcggtga gcgccatcgc gcaggcagcc
     9061 atggcgccgg aaccggcggc ctgcacgaag cgtccggcca ccaggacgcc gaggttcggc
     9121 gcgacgaggc agatcaccgc gccgacggcg agcgccagcg agccgaggag cagcgcggcg
     9181 cgcacgcccc gggtgtcggc gaggcggccg aagagcgccg tgccgacgcc gagcgccagc
     9241 gcgtgcgcgg tcaggaccca ggccgtggcg gtggccgagg cgtcgagcga ggacgccacg
     9301 tcaggcaggg cgaccccggc ggcggtgacg ccgaagacga ccgggccgaa cagggcgccg
     9361 tagcgcagcc cgtccgagcg ggctccgggg gaggaagcgg tgctgacggc cgcggtggac
     9421 gcggcctcgt gcgtggtcat gatgcactcc aaatagtgac gagaacctgc caagaacgag
     9481 cggctcgcgc ggccggacga gcggtggcgg ccgccttacg gaagcggcgc gatgttgtgg
     9541 ttgagccgga agaggttgtc cggatcgacc gcggccttca cccgcgcgag ccggctcaag
     9601 gtctccgggg cgaagcactc cgccgccgat ccggccgtat ccgcgccggc gaggaagttc
     9661 agatagcggc ggcccgtgcc ccactcctcc atcgccccga ggaccacgtc cggcgaaccg
     9721 ggcgccgaga gcgtcgagag cgtgtacgcg ccttcgcgga cgccgaccgc gttcggcacc
     9781 tcggcgggcc gggcgagcgc gccgccgagc tggcgcacct cgaccatcag gtcaccgcag
     9841 gacgaaccgg ccccggccac ccgcagcagc gcctccatgg cctgcgatcc caactcccgc
     9901 agcacgatgt tgcgttcgtg gtacgggagc gggtgcgccg ggtcctggtg gatctcgccc
     9961 acctcggtgt acggcatctc ggcgacggag tccgccagcg gcgtcccgca ctcgcgcagc
    10021 gggcggacga gccgctcgcc ctcctcggcg cgcccggtga aggagatccg cacatgcgcg
    10081 gcgaacgttc cgcgcaggaa ctccggcacg tccggcacgt ccggcagccg cagcagcgag
    10141 acggaggagg tcatctcctc cggcacggtc gcggaccact gccgccaggt gtggagcacc
    10201 tgcgccgcct gctcacccgg gaagtagagg ccgccgccgt agagccggcg caccggcatc
    10261 agaccgaacc agagaccggt gacgatgccg aagttgccct tgccgccgcg cagcgcccag
    10321 aacaggtcgg ggtgccggtc gcgcgtgacc tgccggacgc cgccgtcggc ggtgaccagc
    10381 t
//
