LOCUS       NZ_KB891311            10951 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. ScaeMP-e10 B061DRAFT_scaffold_5.6, whole genome
            shotgun sequence.
ACCESSION   NZ_KB891311
VERSION     NZ_KB891311.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. ScaeMP-e10
  ORGANISM  Streptomyces sp. ScaeMP-e10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 504042)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Improved High-Quality Draft of Streptomyces sp. ScaeMP-e10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 504042)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 504042)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. ScaeMP-e10
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            Isolation Site                    :: Gulf of Mexico
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,185
            CDSs (total)                      :: 7,109
            Genes (coding)                    :: 6,996
            CDSs (with protein)               :: 6,996
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 1 (5S, 16S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 23 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 456788
            Orig. end                         :: 467739
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB891311.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405355
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10951
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="6"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10951
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10951
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5951](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5951
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(480..1805)
                     /locus_tag="B061_RS0129605"
     CDS             complement(480..1805)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132971.1"
                     /locus_tag="B061_RS0129605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_018515152.1"
                     /transl_table=11
                     /translation="MTRTAGAGSARRGSPARRGLRRLQLRLTAAYTLITLVGLSCLSWL
                     VIRTDDRSREAAEYDEMRRRASVAASLVYYEDDRIRLDGLADDEATAGTPQIMVLEERP
                     GKGPAVVFRGSVQQFDVPAAALARAAGSAMRTEEPVRQEARDRTGGPVRLLAVPFWHDA
                     TDEVAGAAVAVGDPAYGSAEHRSLVLSLVVGCAALTALAALTGHVLSGRSMRPAWQALE
                     QQERLLADAAHELRTPVAVMRGSVEMAERGSGEPAAHLPRIRRAADRMGHVVENLLTRG
                     RLEAAVESVRAEPLRLDQLVEEVCADLPEGGHRLELRLEESVVDADAALVRVAVRNLLD
                     NAVRHGRTPGEPGGAELLVTVRGPEVSVADRGPGVEPGRLPELMERFRSPGGGTGIGLS
                     LVQRVALAHRGGLTVRARTGGGAVFVLRLAPARSRRRPKGDR"
     gene            complement(1802..2464)
                     /locus_tag="B061_RS0129610"
     CDS             complement(1802..2464)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132972.1"
                     /locus_tag="B061_RS0129610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_026242044.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLRAVLAAGLRDAGFAVDGADDWPEADVMLHVTAY
                     DCVVLDRMLPSGDSLARLESRRRAGWSVPVLCLTALDGLDDRVRGLESGADDYLAKPFA
                     MRELVLRVRSLTRRAAERLPAFLSCADVVMDLARREVRRGGVLLSLSPKEYAVLQQLLV
                     HRDSVVTRTALLEHCWDEMADPVSNVVDAVIAGLRRKLGTPSLVHTVRGQGFLLGAA"
     gene            complement(2569..3498)
                     /locus_tag="B061_RS0129615"
     CDS             complement(2569..3498)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971209.1"
                     /locus_tag="B061_RS0129615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_026242045.1"
                     /transl_table=11
                     /translation="MMSELPLDQVRTLLAVVDEGTFDAAANALHVTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVLVRFARQLARLEQDAQSALGMSGPGEPTRLTVAVNSDS
                     LATWFLPALGRVPEELGICYELRREDQDHTAALLREGLVMAAVTSSPEAVSGCSVRSLG
                     RMRYLPVATAAFAGRWLGLGTATPLRELIADAPVVDFDRRDDLQDSFVRALGRGARAGS
                     RRHFVPTSEGFVDAVVAGMGWGMLPDVQARPLLRAGRLVALAPDRHVDVPLYWQQWKLD
                     SPALATVAEAVVAEAAEALDGAPGSTGP"
     misc_feature    complement(3515..3528)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.13"
                     /tool="antismash"
     gene            3565..4176
                     /locus_tag="B061_RS0129620"
     CDS             3565..4176
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="B061_RS0129620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_018515155.1"
                     /transl_table=11
                     /translation="MTNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRREAVSAVVVI
                     CALSDAVLIALGVAGVGAVVVAWPSALTVVSLLGGVFLIIYGLLAARRAFRPSALHPQD
                     APSGAKRGVVLACLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAILASVCWFAA
                     LGFGARLLSGVLARPSSWRVLDGLVAAMMIGLGARLALTA"
     gene            complement(4217..4855)
                     /locus_tag="B061_RS0129625"
     CDS             complement(4217..4855)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606642.1"
                     /locus_tag="B061_RS0129625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_018515156.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQAILMAAAETFEKHGYQAATITEILKIAGVTKGA
                     LYFHFPSKEELALGVIEAQDPPPPTPDQPLKLQELVDMGMLFSHRLHTSLLTRAGVRLS
                     MDQQAQGLDRTGPFLRWQQTTHDLLAQAKQNGELLPHVNPTETADLYVAAFTGIQAVSQ
                     TLTNYRDLEQRYVTLQRHILPSIANPSILTALDLSPERTTRLARLIPAY"
     misc_feature    4982..4997
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 23.65"
                     /tool="antismash"
     gene            5001..5951
                     /locus_tag="B061_RS0129630"
     CDS             5001..5951
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="B061_RS0129630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_026242046.1"
                     /sec_met_domain="AfsA (E-value: 8.3e-27, bitscore: 87.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLTDPVAASAVGSEARVVHPVGMELVHRSRAEDAFPRNWE
                     RLGPDRFHVEAVLPHDHPFFAPVGADRHDPLLVTEAMRQAAMLAFHAGYGVPVGYHFLM
                     ADLDYATHPDHLVVGGLPTVIDVEVTCSELKWRGGLPVQGRVDWVVHRAGRLVATGMGA
                     TRFTSPRVYRRIRGGSAEPGVAIPRTAPVDPARAGRARAEDVVLSESSREGVWELRVDT
                     RHPTLFQRPNDHVPGMLLLEAARQAACLAAGDGGFVPAEGRTHFHRYAEFGSPCSIAAE
                     VLPGGPPGEARIRVTGHQDGEPVFETVLTGPVTAG"
     gene            6032..6892
                     /locus_tag="B061_RS0129635"
     CDS             6032..6892
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491075.1"
                     /locus_tag="B061_RS0129635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_018515158.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGRETARLLAAAGPVRILARRPERVTVRGPEVDIV
                     AGEYGDRASLDRALRDIDAVFLVTNSPTEPDDARVAAAAAAAGVRHLVKLSMMAVEEPD
                     ADDFITRLQRGNEQAIRESGVPWTFVRPRTFMSNTLSWAKGIASDGVVRALHGRSPVAC
                     VDPRDVAAVAVAALTGTGHEGRAYGVSGPEAISAYEQTAQLSEMLGRPLRFEELPLDRA
                     REALLAKYPQPVAEAFLESAERQRAGAKAAVVPTVEELTGRPARPFRTWAADHAESFGA
                     AWRPA"
     gene            7059..7745
                     /locus_tag="B061_RS0129640"
     CDS             7059..7745
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515159.1"
                     /locus_tag="B061_RS0129640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_018515159.1"
                     /transl_table=11
                     /translation="MTKQVRAARTRQALIRSAAIVFEQHGYGQARLALISSGAGVSTGA
                     LHFHFENKAAVAQAVVAEASHGLLEVSGSIRRRTDTALQTLVDTSHALVDLLGRDPVTR
                     AGFRLSYGGERGGEPGLPAQWQSCVKELLDEAAAVGTLMEGVSRTDVTAATVAATTGFE
                     VLGRCDSQWLSARTLTGFWRLLLPCLAAPEVLPLLDPSGTVAATEAVATGRPERETYGM
                     ETAAPI"
     gene            complement(7755..8186)
                     /locus_tag="B061_RS0129645"
     CDS             complement(7755..8186)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761874.1"
                     /locus_tag="B061_RS0129645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_018515160.1"
                     /transl_table=11
                     /translation="MEHTTDPYELRVGVPSVELFRRLRTDAGLSDKAPEAVAIALPNTW
                     HGVVLHHEGEPIGMGRIIGDGGTAFQVVDICVHPAHQGRGLGRRIMAALTEELERRAPA
                     TAYVSLIADGPARHLYEKFGFADTAAHDSIGMYRSMGAG"
     gene            complement(8218..9165)
                     /locus_tag="B061_RS0129650"
     CDS             complement(8218..9165)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606638.1"
                     /locus_tag="B061_RS0129650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_086018915.1"
                     /transl_table=11
                     /translation="MGSMDVSPDPAAPEPLDRRAELSEFLRTRRARLQPQDVGLPEFGR
                     ARRVPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLSDAEHAHLTHM
                     AKPAQHKKKRRPARVQRVRTGLLHLLDSLEGIPAYVSGARSDILAWNPMAAAVFGDWGA
                     LPPEERNWARLVFLSPAYRDLFVEWDSKASDMVSYLRLYAGCHPDDPQLSALVGELSVK
                     NDEFRRLWATHNVKEKGHGTKLIRHPLVGDMALSYETLHLPDDPEQYVVTYHAEPDSAS
                     SEALRLLASWGADARRADVERVDPGGRAGRGRAG"
     misc_feature    complement(9232..9247)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     gene            9310..10350
                     /locus_tag="B061_RS0129655"
     CDS             9310..10350
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 2.7e-12.
                     Score: 38.4. Matches aSDomain:
                     nrpspksdomains_B061_RS0129655_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="B061_RS0129655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_018515162.1"
                     /sec_met_domain="ADH_N (E-value: 2.3e-29, bitscore: 96.2,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2e-22, bitscore:
                     74.3, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDVLIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVTETGPGVTKFKAGDRVGVGCMVDSCGKCDACLMGR
                     EQHCVEGNTQTYNALDRSGEPTYGGYSTYIVVTEGFVVGIPEGIALDEAAPLLCAGITT
                     YSPLRRWGAGPGRKVAVIGLGGLGHMAVKIAHTLGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPQTFEELAGTFDIILSTVSAPLDFGAYLALLRTDGTLVNVGAPEEPVSLNLFSL
                     IGGNKSLAGSAIGGIAETQEMLDFCAVHGLGAEIEVIEAGQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        9367..10116
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B061_RS0129655_PKS_ER.1"
                     /evalue="2.70E-12"
                     /label="B061_RS0129655_PKS_ER.1"
                     /locus_tag="B061_RS0129655"
                     /protein_end="269"
                     /protein_start="19"
                     /score="38.4"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVTETGPGVTKFKAGDRVGVGCMVDSCGKCDACLMGREQHCVEGNTQTYNALDRSG
                     EPTYGGYSTYIVVTEGFVVGIPEGIALDEAAPLLCAGITTYSPLRRWGAGPGRKVAVIG
                     LGGLGHMAVKIAHTLGAEVTVLSQSLRKKDDGLKLGADHYYATSDPQTFEELAGTFDII
                     LSTVSAPLDFGAYLALLRTDGTLVNVGA"
     CDS_motif       9493..9528
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B061_RS0129655_0001"
                     /evalue="1.10E-02"
                     /label="PKSI-ER_m2"
                     /locus_tag="B061_RS0129655"
                     /protein_end="73"
                     /protein_start="61"
                     /score="8.9"
                     /tool="antismash"
                     /translation="GHEIAGIVTETG"
     gene            complement(10424..10786)
                     /locus_tag="B061_RS0129660"
     CDS             complement(10424..10786)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491070.1"
                     /locus_tag="B061_RS0129660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_018515163.1"
                     /transl_table=11
                     /translation="MPTTEPEVAAPSGRRLAHPSTEEIRLEGVLHALADPVRLTIVREL
                     ADGHADMACIAFDLPVSKSTTTHHFKVLREAGVIRQHYEGTSRMSRLRETDLEARFPGL
                     LAAVLEAARRSVAPSA"
ORIGIN
        1 tggcggaggt cgaggtgtcg agcagcgcct cgccccaggc ctcgtcgttg accggcttgc
       61 cgctgccgtc gcgcagcagc aggtcctggt tcttcttctg ccaccagtcc atcgcgtccg
      121 gctgggtctg gaaggcgttg acgtaacaga cgttgtacag accgcgtacg ggcttggcgc
      181 cccggtcgcg cgagagcgcc ttgaccccgg cgggcggggt gtaggccccg ccgatctggt
      241 agtcgaacgt ggcgtcggcc ttggggagga cgaccttcgg cgcgggcttg gccgcgaagg
      301 cgctggagag caggaacccc gccccggctg tcagggcgac cccgccggcc agggccgcga
      361 gggtggagcg gcgacggagg aagggggtgg cggcgtgctg gggcatgggg ggctcctgtg
      421 cgtggtctgc gtacctgccc ccgcatcatg gactgccgat catgagcgaa tcgtgagcgt
      481 cacctgtcgc ccttcggtct ccgccgtgac cgggccggcg ccagccgcag cacgaacacc
      541 gcaccgccgc cggtccgggc ccgcacggtc agcccgcccc ggtgggcgag ggccacccgc
      601 tgcaccagcg agaggccgat gcccgtaccg ccgcccggcg agcggaagcg ctccatcagc
      661 tcgggcagcc gtccgggctc cacccccgga ccgcggtcgg cgaccgagac ctccggtccg
      721 cgtacggtca cgagcagttc ggccccgccc ggctccccgg gcgtacggcc gtggcgtacg
      781 gcgttgtcga gcaggttgcg gacggccacc cgcaccagcg ccgcgtccgc gtcgaccacc
      841 gactcctcca gccgcagctc cagccggtgc ccgccctccg gaagatcggc gcacacctcc
      901 tccacgagct ggtccagccg caacggctcg gcccgcaccg actccacggc ggcctccaac
      961 cgcccccggg tcagcaggtt ctccaccaca tggcccatcc ggtccgccgc ccgccggatc
     1021 ctcggcagat gggccgccgg ctcgcccgaa ccgcgctcgg ccatctccac cgagccgcgc
     1081 atcaccgcca ccggggtgcg cagctcgtga gctgcgtcgg cgagcagccg ctcctgctgc
     1141 tccagcgcct gccaggcggg ccgcatggac cgcccggaga gcacatggcc ggtgagcgcg
     1201 gccagtgcgg tgagggcggc gcacccgacg acgagggaga ggaccaggct tcggtgctcg
     1261 gccgacccgt acgccgggtc cccgaccgcg acggccgcgc ccgccacctc gtcggtcgcg
     1321 tcgtgccaga agggcacggc cagcagccgg acgggaccgc ccgtacggtc gcgggcctcc
     1381 tgccgcaccg gctcctcggt gcgcatcgcc gaacccgccg cccgggcgag tgctgcggcc
     1441 ggtacgtcga actgctggac gctgccccgg aacaccacgg ccgggccctt gcccggccgc
     1501 tcctccagca ccatgatctg cggcgtgccc gcggtcgcct cgtcgtcggc caggccgtcg
     1561 aggcggatgc ggtcgtcctc gtagtagacg agcgaggcgg cgacggaggc ccggcggcgc
     1621 atctcgtcgt actcggctgc ctcccgggac cggtcgtcgg tgcggatcac cagccaggac
     1681 aggcagctca gcccgacgag ggtgatcagc gtgtacgcgg cggtcagccg cagctggagc
     1741 cggcgcagcc ctctccgggc cggggagccg cgccgcgcgg acccggcgcc tgccgtgcgg
     1801 gtcatgccgc gcccagcagg aacccctggc cgcgcacggt gtgcacgagc gacggagtgc
     1861 cgagcttgcg ccgcaggccc gcgatcacag cgtccaccac gttggagacc gggtcggcca
     1921 tctcgtccca gcagtgctcc agcagcgccg tacgcgtgac gacgctgtcc cggtgcacca
     1981 ggagctgctg gagcactgcg tactccttcg ggctcagcga gagcagcacc ccgccccgcc
     2041 gcacctcccg ccgcgccagg tccatcacca cgtccgcgca gctcaggaag gcgggcagcc
     2101 gctcggccgc ccgccgggtc aggctgcgca cccgcaggac gagctcgcgc atcgcgaacg
     2161 gtttggccag gtagtcgtcc gccccgctct ccagcccgcg cacccggtcg tccaggccgt
     2221 ccagagcggt gaggcagagc acgggcaccg accagcccgc ccggcgccgg ctctccagcc
     2281 gtgcgaggga gtcgccggag ggcagcatcc ggtccaggac cacacagtcg tacgcggtca
     2341 cgtgcagcat cacgtccgcc tccggccagt cgtccgcacc gtcgacggcg aagcccgcat
     2401 cgcgcagccc cgcggcgagc accgcccgca ggtcgtcgtc gtcctccacc agcagcacgc
     2461 gcaaggagac gcctccgggt ctctcgggat ggcccccggg cgcttcccgt acggaaccgg
     2521 gagccctgac ctgggggaga gacccgccat cctaaggggc ccgtcgagct aggggcccgt
     2581 cgagccagga gccccgtcga gcgcctcggc ggcctccgcc actacggcct ccgccacggt
     2641 ggcgagggcc ggtgagtcca gcttccactg ctgccagtac agggggacgt ccacgtgacg
     2701 gtccggggcg agggcgacca gccgcccggc gcgcagcagc gggcgcgcct ggacgtcggg
     2761 cagcatgccc cagcccatcc cggccaccac ggcgtccacg aacccctccg acgtgggcac
     2821 gaagtggcgc cgggagcccg cccgcgcacc gcgcccgagc gcgcggacga aggagtcctg
     2881 gaggtcgtcg cgccggtcga agtccaccac cggggcgtcc gcgatcagct cgcgcagcgg
     2941 ggtcgccgtc cccagcccga gccaccgccc ggcgaaggcc gccgtcgcca ccggcagata
     3001 gcgcatccgg cccaggctcc gcaccgaaca gccggacacc gcctcgggcg aggaggtcac
     3061 cgccgccatc accagcccct cgcggagcag cgccgccgta tggtcctggt cctcccggcg
     3121 cagctcgtaa cagatgccca gctcctccgg cacgcgcccg agggcgggca ggaaccaggt
     3181 cgccagggaa tcggagttca ccgcgaccgt cagccgcgtc ggctcgccgg ggcccgacat
     3241 cccgagggcc gactgcgcgt cctgctccag ccgggccaac tggcgggcga aacgcaccag
     3301 gacctcaccg gactcggtgg gccgcaccgg tttcgtccgc atcagcagca cccgcccggt
     3361 gcgctgctcc agggccttca cgcgctggct gaccgccgac ggcgtcacat gcagggcgtt
     3421 ggccgccgcg tcgaaggtgc cttcgtcgac gacggccagc agggtgcgca cctggtcgag
     3481 aggaagctca gacatcacaa cagctaatga tacgtaagaa tcattagttg tacatggatg
     3541 cgggatctcc ctagcgtcgg caccatgacc aacgctctga ccactgcggc cgccgggttc
     3601 ggtaccggcc tctccctcat cgtcgccatc ggcgcccaga acgccctggt cctgcgccag
     3661 ggcgtccgcc gggaggccgt atccgcggtg gtggtcatct gcgccctgtc cgacgccgtc
     3721 ctcatcgccc tcggagtcgc cggggtcggt gccgtcgtcg tggcctggcc gtccgccctg
     3781 accgtggtct ccctgctggg aggggtgttc ctgatcatct acggcctcct ggccgcacgc
     3841 cgggccttcc gcccctccgc cctgcacccg caggacgcgc cctccggggc gaagcggggc
     3901 gtcgtgctcg cctgcctcgc gatgacctgg ctcaacccgc acgtctacct ggacaccgtc
     3961 ttcctgctcg gctccgtcgc cgccgaccgc ggcccgctgc gctggacctt cgggctgggg
     4021 gcgatcctcg ccagcgtgtg ctggttcgcc gctctcggct tcggcgcccg gctgctcagc
     4081 ggggtgctcg cccgcccgtc gtcgtggcgt gtcctggacg ggctggtcgc cgcgatgatg
     4141 atcggcctcg gcgctcggct ggccctgacc gcctgaccgc ctgaccgcct gcccccctgg
     4201 ccggccgtcg gcgggctcag tacgcgggga tgagccgggc gaggcgggtg gtgcgctcgg
     4261 ggctgaggtc cagcgcggtg aggatggagg ggttggcgat gctgggcagg atgtgccgct
     4321 ggagcgtgac gtaacgctgt tccagatcgc ggtagttggt gagggtctgc gagaccgcct
     4381 gaatgccggt gaaggccgcg acgtagaggt cggcggtctc cgtggggttg acgtgcggga
     4441 ggagttcgcc gttctgtttg gcttgggcga gcaggtcgtg ggtggtctgc tgccacctga
     4501 ggaaggggcc ggtgcgatcc aggccctggg cctgctggtc catggagaga cggacaccgg
     4561 cacgggtgag gaggctggtg tggagacggt ggctgaacag catccccatg tccacgagct
     4621 cctggagctt gagcggttgg tccggagtgg gcggtggggg gtcctgggcc tcgatcacac
     4681 cgagcgcgag ctcttccttg gacgggaaat ggaagtacag cgcgcctttg gtcactccgg
     4741 cgatcttgag tatctcggtg atggtggcgg cctggtaacc gtgcttctcg aaggtctcgg
     4801 cggctgccat caggatggcc tggcgggtac ggatcgcccg gtcctgcttg gccatctctg
     4861 tctccttcgt cgggcggtgc cggtcgtggg gccgggcggg cagccggtgg ggatagaaaa
     4921 aaaaccgctt agtctgtatc ttatcagcgc gtgcgcaccc agaaacgact cgcccgtcac
     4981 atgacccctg ggggggagtt atgcccgaag cagcagtctt gaccgaccct gtagcggcgt
     5041 cggccgtggg ctcggaggcc cgagtggtgc atccggtggg aatggaactg gttcacagga
     5101 gcagggcgga ggacgccttt ccgcgcaact gggagcggct ggggccggac cggttccacg
     5161 tggaggctgt gcttccgcac gatcatccgt tcttcgcccc ggtgggagcg gaccgtcacg
     5221 atccgctgct ggtgacggag gcgatgcggc aggcggcgat gctggccttc cacgccgggt
     5281 acggagtccc cgtcggctac cacttcctga tggccgatct ggactacgcg acccaccccg
     5341 atcacctggt cgtgggcggc ctgcccaccg tgatcgacgt ggaggtgacc tgctccgagc
     5401 tcaagtggcg cggcgggctg ccggtgcagg gccgcgtgga ctgggtggtg caccgcgcgg
     5461 gccggctggt cgcgaccggg atgggggcga cgaggttcac cagcccgcgt gtctaccggc
     5521 ggatccgcgg cggatcggcg gagccgggcg tggcgatacc gcggacggcg ccggtggacc
     5581 ccgcccgggc ggggcgcgcc agggcggagg acgtggtgct ctcggagagc tcccgcgagg
     5641 gggtctggga gctgcgggtg gacacccgtc acccgacgct gttccagcgg cccaacgacc
     5701 atgtgccggg gatgctgctg ctggaggcgg cgcggcaggc ggcgtgcctg gccgccgggg
     5761 acggcgggtt cgtccccgcc gaggggcgta cgcacttcca ccggtacgcg gagttcggca
     5821 gcccgtgctc gatcgccgcg gaggtcctgc cgggcggccc gccgggcgag gcgaggatcc
     5881 gggtgacggg ccatcaggac ggcgagccgg tcttcgaaac ggtgctgacg ggccctgtga
     5941 cggcgggctg aggagcagcg tccggcgcgc gtgtgagcgg tgtgttgcgc ctggacgaca
     6001 ggctgatgac ggtgtgtgag gagtacgtga cgtgattctg gtgacagggg cgacgggggc
     6061 ggtcggccgt gagaccgccc gtctgctggc ggccgccggt ccggtgcgga tcctggcgcg
     6121 gcggccggag cgcgtgacgg tgcgcggacc cgaggtcgac atcgtggcgg gggagtacgg
     6181 ggaccgcgcg tccctcgacc gggcgctgcg ggacatcgac gcggtgttcc tcgtgacgaa
     6241 cagccccacg gagcccgatg acgcgagggt cgccgcggcg gccgcggcgg caggagtgcg
     6301 ccacctcgtc aagctgtcga tgatggcggt cgaggagccg gacgcggacg acttcatcac
     6361 ccggctccag cgcggcaacg aacaggccat cagggagtcc ggggtgccgt ggaccttcgt
     6421 ccgccccagg acgttcatgt ccaacacgct ctcctgggcg aagggcatcg catcggacgg
     6481 tgtcgtgcgg gccctccacg ggcggtcgcc ggtcgcgtgc gtcgatcccc gggacgtggc
     6541 ggcggtggcc gtcgcggccc tcaccggtac gggccacgag ggacgggcgt acggggtgtc
     6601 gggcccggag gcgatcagcg cgtacgagca gacggcccag ctctccgaaa tgctgggccg
     6661 gccgctgcgc ttcgaggaac tgcccctgga ccgcgcgcgc gaggcgctgc tcgcgaagta
     6721 tccgcagccg gtggccgagg cgttcctgga gagcgcggag cgccagcggg ccggggccaa
     6781 ggcggcggtg gtgcccaccg tcgaggagct gaccggccgc cccgccaggc cgttccgcac
     6841 ctgggccgcg gaccacgcgg agtccttcgg cgcggcctgg cgaccggcgt agggacgcgg
     6901 gtgccgaccg cgcatacgtt cccctggaaa tcaaaccggt aagttggttt gatattcctc
     6961 atgacgctgc ttcaccgctc cggcggagcg cccccggcct tcggagccca cagcccgggg
     7021 ccccatccca tccgtttctc ctcccggcgg aggccgcggt gaccaagcag gtgcgcgcag
     7081 cgcgtacccg ccaagccctg atccgctcag cggcgatcgt gttcgagcag cacgggtacg
     7141 gacaggcgcg cctggcgctg atcagttccg gcgcgggcgt cagcacgggt gccctgcact
     7201 tccacttcga gaacaaggcc gcggtcgccc aggccgtggt cgcggaagcg tcgcacggtc
     7261 tgctggaggt gtccggctcc atccgccgca ggacggacac ggccctgcag accttggtcg
     7321 acacctcgca cgccctggtg gacctgctcg gccgcgatcc ggtgacacgg gccggattcc
     7381 ggctcagcta cggcggcgag cggggcggtg aacccggcct gcccgcgcaa tggcagagct
     7441 gcgtcaagga gctgctcgac gaggcggccg ccgtcggcac cctcatggag ggcgtctccc
     7501 gcacggacgt caccgccgcg acggtcgccg cgaccaccgg gttcgaggtg ctcggacggt
     7561 gcgactccca gtggctttcc gcccggacgc tcaccggctt ctggcggctg ctgctgccct
     7621 gcctcgccgc cccggaggtc ctgccgctcc tggacccgtc ggggaccgtc gcggccacgg
     7681 aggccgtcgc cacgggccgg cccgaacgcg agacgtacgg gatggagacg gccgcgccga
     7741 tctgacctcg cggctcagcc cgcgcccatc gagcggtaca taccgatcga gtcgtgcgcg
     7801 gcggtgtcgg cgaagccgaa cttctcgtac aggtggcgcg cgggcccgtc cgcgatcagt
     7861 gagacgtacg cggtggcggg cgcccggcgt tcgagctctt ccgtgagcgc ggccatgatg
     7921 cgcctgccga gcccccggcc ctggtgcgcg gggtgcacgc agatgtcgac cacctggaac
     7981 gcggtgccgc cgtcaccgat gatccgcccc atgccgatcg gttcgccctc gtggtggagg
     8041 accacgccgt gccaggtgtt gggcagggcg atcgcgaccg cctcgggcgc cttgtcggag
     8101 agcccggcgt cggtgcgcag tcggcggaag agctcgacgg acggtacgcc cacccggagc
     8161 tcgtaagggt cagtggtgtg ttccatgcat tacacgatag agcaacggcc ggtgcgccta
     8221 tccggcccgg ccccggcccg ccctgccgcc cgggtcgacc cgctccacat ccgcccgccg
     8281 cgcgtccgca ccccagctcg ccagcagccg cagcgcctcg gacgaggcgg agtccggttc
     8341 cgcgtggtag gtcaccacgt actgctccgg atcgtccggc aggtgcagcg tctcgtacga
     8401 cagggccatg tccccgacga gcggatgccg gatcagcttg gtgccgtggc ccttctcctt
     8461 cacgttgtgc gtggcccaca gccgccggaa ctcgtcgttc ttgacggaga gttcacccac
     8521 cagcgcggag agctgcggat cgtccgggtg gcagcccgcg tacaggcgca gatagctgac
     8581 catgtccgag gccttggagt cccactccac gaacaggtcc cggtaggcgg gggagaggaa
     8641 gacgagccgc gcccagttcc gctcctcggg cggcagcgcg ccccagtcgc cgaagaccgc
     8701 cgccgccatc gggttccagg cgaggatgtc ggagcgggcc ccggagacgt acgcgggaat
     8761 gccctccagg ctgtccagga ggtgcagcag tcctgtccgc acccgctgca cccgcgccgg
     8821 gcggcgcttc ttcttgtgct gggcgggctt ggccatgtgc gtcagatgcg cgtgctcggc
     8881 gtcgctgagc cgcagcgccc gggcgatcgc gtcgagcacc tcggccgaga cgttgcgccc
     8941 gttgccctgt tccagccggg tgtagtacgc caccgagacc ccggccagct gcgccagctc
     9001 ctcccgccgc agccccggca cccggcgggc ccggccgaac tccggcagcc ccacgtcctg
     9061 cggctggagc cgggcccgcc gggtgcgcag gaactcgctg agctcggcgc gccggtcgag
     9121 cggctcgggg gcggcggggt cgggcgaaac gtccatggaa cccattattc ctggtcgtac
     9181 gcgcgggagc ctgtccccgc cagtagtagg accactggac gtaggcaaag tggtgacctg
     9241 ggtaaatgct gtgcgccccc tgatgctggg agacgtactc accgcatcac ccagaggaga
     9301 acctccggca tgaccaccgt tgccgcttac gccgctcccc gtgccaaggc cccgctggag
     9361 cgcaccacga tcgagcgccg cccggtgggc gagttcgacg tcctgatcga catcaagttc
     9421 gccggtatct gccactccga catccaccag gcccgcgacg gctggggcga gggcatcttc
     9481 ccgatggtgc ccggccacga gatcgccggg atcgtcaccg agaccggccc cggcgtcacc
     9541 aagttcaagg ccggcgaccg cgtcggcgtc ggctgcatgg tcgactcctg cggaaagtgc
     9601 gacgcctgtc tgatgggccg cgagcagcac tgcgtcgagg ggaacaccca gacgtacaac
     9661 gccctcgacc gcagcggcga gccgacctac ggcggctact cgacgtacat cgtcgtgacc
     9721 gaggggttcg tcgtcggtat ccccgagggc attgccctgg acgaggccgc gccgctgctg
     9781 tgcgccggca tcaccacgta ctccccgctc cgccgctggg gcgccggccc gggcaggaag
     9841 gtcgcggtca tcggcctcgg cgggctcggc cacatggccg tcaagatcgc gcacaccctc
     9901 ggcgccgagg tgacggtgct ctcccagtcg ctgcgcaaga aggacgacgg gctcaagctg
     9961 ggcgccgacc actactacgc gaccagcgac ccccagacct tcgaggagct ggccggaacc
    10021 ttcgacatca tcctctccac ggtctcggcc ccgctggact tcggcgccta cctcgccctg
    10081 ctgcggacgg acggcaccct ggtcaacgtg ggcgcgcccg aggagccggt ctcgctcaac
    10141 ctcttctcgc tgatcggcgg caacaagtcg ctggcgggct ccgcgatcgg aggcatcgcc
    10201 gagacccagg agatgctgga cttctgcgcc gtgcacggcc tcggcgccga gatcgaggtc
    10261 atcgaggcgg gccaggtcaa cgaggcgtac gagcgggtcc tcgccagcga cgtccgctac
    10321 cgcttcgtga tcgacaccgc gaccatctga gcgatctgtt cagcagttgt acgccgtgtg
    10381 ccccgggccc tcgaaagggc ccggggcaca cggcgtaaag gacttatgcg gaaggcgcga
    10441 ccgagcgccg cgccgcttcc aggacggccg ccaaaagccc ggggaagcgc gcctccaggt
    10501 cggtctcgcg cagcctgctc atccgggagg tgccctcgta gtgctggcgg atgacgcccg
    10561 cctcgcgcag caccttgaag tggtgggtgg tggtggactt gctcaccggg aggtcgaacg
    10621 cgatgcaggc catgtccgcg tggccgtcgg ccagctcgcg cacgatggtc agccggaccg
    10681 ggtcggccag cgcgtgcagg acgccctcca gacggatctc ctccgtggac ggatgggcga
    10741 gccggcgccc ggacggggcg gcgacctcgg gttcggtcgt gggcatgggc ggcctcctca
    10801 ggtctcgcat ctctaaagta cgaacatcct cgtagtttga caactcccgt actacgaggt
    10861 ctatcgtact ggccatggcg gtcctgccct tccagcccgg gcagggcgcc ggctccgtgc
    10921 cccgacccct tgaggagacg ttccggtgag t
//
