LOCUS       NZ_KB891310            11296 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. ScaeMP-e10 B061DRAFT_scaffold_4.5, whole genome
            shotgun sequence.
ACCESSION   NZ_KB891310
VERSION     NZ_KB891310.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. ScaeMP-e10
  ORGANISM  Streptomyces sp. ScaeMP-e10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 515152)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Improved High-Quality Draft of Streptomyces sp. ScaeMP-e10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 515152)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 515152)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. ScaeMP-e10
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            Isolation Site                    :: Gulf of Mexico
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,185
            CDSs (total)                      :: 7,109
            Genes (coding)                    :: 6,996
            CDSs (with protein)               :: 6,996
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 1 (5S, 16S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 23 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 189928
            Orig. end                         :: 201224
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB891310.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405355
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11296
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11296
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11296
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6296](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6296)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(40..54)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    399..408
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            1300..2028
                     /gene="hemQ"
                     /locus_tag="B061_RS0126375"
     CDS             1300..2028
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="B061_RS0126375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_026241928.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAKDITVRGTYDVSGLRADADLMIWWHAETSDELQEAYNLFRRTKL
                     GRALEPVWSNMALHRPAEFNKSHVPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWVLAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     gene            complement(2196..3764)
                     /locus_tag="B061_RS0126380"
     CDS             complement(2196..3764)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-286). E-value:
                     8.3e-16. Score: 50.3. Matches aSDomain:
                     nrpspksdomains_B061_RS0126380_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="B061_RS0126380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_018514513.1"
                     /sec_met_domain="PF00561 (E-value: 9.6e-22, bitscore: 72.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVPLYATIGSLVLSALAAVPATADGGPGPAASAEERGTAVAAA
                     RASAAGISFGACPEEEMLPDTLKCGTVSVPLDYARPDGRTIDLTVSRTLATGPAAGRQG
                     SFVYNPGGPGASSITFPLMGEMSEWKAVAEAYDMVGYAPRGVGASAPLSCQNPAAFAKS
                     PTDSPAHPTTAYKEQRIAQARAYARGCEKHAGEALRHYTSLNNARDLEVLRAALGDERL
                     TFMGASYGTYLGSLYATLFPSRVRRMVFDSAVDPDPEQIWYRSNLDQSLAFESRWEDFR
                     RWVAKHDDVYGLGPTPEAVQGHYEDVRTALATEPAGGKVGPGQFHAAFREAAYYDDYWA
                     MRATALSEYVRGNPEPLVTQMSPRAITAKDDENARAVYTAVECNDAPWPEEWEVWDRDH
                     TDLARIAPFQTWDNAFTNLPCAFWPAPRQQPLDVSTGWGELPPVLILAAERDAATPYKG
                     ALELQRRLRGAVLVTERDAGTHGVGGAGNTCVDEHLRRYLLTGATPDGDAECAPRAEPN
                     PVSLD"
     aSDomain        complement(2907..3449)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B061_RS0126380_Abhydrolase_1.1"
                     /evalue="8.30E-16"
                     /label="B061_RS0126380_Abhydrolase_1.1"
                     /locus_tag="B061_RS0126380"
                     /protein_end="286"
                     /protein_start="105"
                     /score="50.3"
                     /tool="antismash"
                     /translation="FVYNPGGPGASSITFPLMGEMSEWKAVAEAYDMVGYAPRGVGASA
                     PLSCQNPAAFAKSPTDSPAHPTTAYKEQRIAQARAYARGCEKHAGEALRHYTSLNNARD
                     LEVLRAALGDERLTFMGASYGTYLGSLYATLFPSRVRRMVFDSAVDPDPEQIWYRSNLD
                     QSLAFESRWEDFRRWVAK"
     gene            complement(3958..4995)
                     /locus_tag="B061_RS0126385"
     CDS             complement(3958..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491674.1"
                     /locus_tag="B061_RS0126385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_018514514.1"
                     /transl_table=11
                     /translation="MDTLALALTLTVALSTVLLLAKVVVSRSRRPSAGPSPTVHDLYEA
                     AFLNGGPARVTDTALTALYADGRVLIGGPGIISVQRAHAQDPVERAVLQALSAAPSGAL
                     HAVREAVMHHPAVQEIGDGLAQRGLLVRPAESRPVRRWALIQGVSCFVAFPLSIILTVI
                     QYTSLEDSFDMPVPFVLKMLPAILLGGIGGFVVAASAANRITRAGRRAAQAFRTANASV
                     LTPPYLVANRGLRALPDPVLQAQLIAAARLHTRTRRSRPGSSGRRSTGYVGGTAGASAF
                     AATTVWCADAGPGHTSCGGGGGGGCGGGSGCGGSGSSCGGSGSSCGGSSGGSSCGGGSS
                     CGSSS"
     gene            complement(5001..6296)
                     /locus_tag="B061_RS0126390"
     CDS             complement(5001..6296)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100703.1"
                     /locus_tag="B061_RS0126390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_018514515.1"
                     /sec_met_domain="DUF692 (E-value: 6.2e-93, bitscore: 305.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLGIGIGWRPEIAAEVEALTGVDWVEVVAENLCADHLPDSLVRL
                     RERGVTVVPHGVSLGLGGADRPDAERLAGLAARAELLGTPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALEVLCENVAIAQDALPVPLALENIAALVSWPEEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVEKNGVWHDTHA
                     HPVTAPVLDVLAELRSRVDPPGVLLERDDAFPPGPELAAELETIRGTLRAAGGPSGVRT
                     GTWTGTTAPPAPARPGARDRTAVAQTALLSALVAGTPAPEGFDHRRLRVQSRALAAKRA
                     DVVAKVAPELPEILGDGYRAAFLDYARTRPMSDGYRRDALDFVERILVHGGLPDPVAQR
                     RLTHWWEDRSGPRPPSRTTRIVRAARAALAGK"
     gene            complement(6407..7153)
                     /locus_tag="B061_RS0126395"
     CDS             complement(6407..7153)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="B061_RS0126395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_018514516.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATVGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHTDLDRRAR
                     DVAAKLGVELPNQPTDQQQAWLNELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEGTGFVDFDALAREAAGGSTASPSGPSMPQGGQVPP
                     VPVPVTPTGDQSFTSRPVPPTMPPMP"
     gene            7435..8265
                     /locus_tag="B061_RS0126400"
     CDS             7435..8265
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059752.1"
                     /locus_tag="B061_RS0126400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NmrA/HSCARG family protein"
                     /protein_id="WP_018514517.1"
                     /transl_table=11
                     /translation="MTDRKIILVLGGTGNQGGATARELLSRGWSVRALVRDPDKPEAQA
                     LKELGAVLVRGDLEDVASLRAAAEGVHGIFSVQALAYEPETLAAEVRHGKAVADVAKEA
                     GVTHFVYSSVGGAERGTGIDHFESKAEIERHIKALGLPATILRPVFFMNNLLHFADVEG
                     ERVMSLPVEPDKPMQFIASDDIGVFAADAFDRPAEFIGREIELAGDEITFPQVAEVYER
                     ITGTPSRLEATPIEERMFAWFAEEGYQADIPALRELHPGLLTFEQFLAQRLGTS"
     misc_feature    complement(7446..7461)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.56"
                     /tool="antismash"
     gene            complement(8283..9041)
                     /locus_tag="B061_RS0126405"
     CDS             complement(8283..9041)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019993147.1"
                     /locus_tag="B061_RS0126405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_018514518.1"
                     /transl_table=11
                     /translation="MSSDDTSQSPARETSPSPFHHGQTDRDAAPQYVLPLVVHLEKTAP
                     PARTDALRTAARAVLTILADERSTGEGEWAGAMRDWEDARIRKVVRRARGAEWRKAAAL
                     PGITVTGGSAEVRVYPPIPLDGWPKELAKLQVSGTDLDDPEPPAAPDPEGPVLWLNPEL
                     EMSAGKTMAQVGHGAQLAWWELSDTERKAWREAGFPLSVATATPERWRELTASGLPVVR
                     DAGFTEIAPGPTVVAEGHHRVGSLPPAQRP"
     gene            9114..9788
                     /locus_tag="B061_RS0126410"
     CDS             9114..9788
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="B061_RS0126410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AIM24 family protein"
                     /protein_id="WP_018514519.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQPATAPGMTLQNSKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMAVRGQGEAWFAHEAANCFILEMDQGDQIT
                     INGRNVLCFDATLSYEIKTVKGAGMIGGGLFNSVFTGYGKLGLMCEGTPIVIPVTGAQP
                     VYVDTDAVVGWSQQLTTTLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     AN"
     gene            9815..9997
                     /locus_tag="B061_RS0126415"
     CDS             9815..9997
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="B061_RS0126415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018514520.1"
                     /transl_table=11
                     /translation="MDDASYCATPAPADPEADAGPPFAECVLCREPTEYPESARGATLC
                     PVCAWQEAGRSACSG"
     misc_feature    complement(10942..10956)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
ORIGIN
        1 ccgagtcgat gctcgcccgc cgccccgagg cggtctcgct ggccgaggcg gtcggcctcg
       61 gcggccggct ccagccgccc gccaccgcga ccgcctccct ctggacgcgc gacgcgctgc
      121 gccccatgcc caaggggcac gtgatgggcg tccccggcga cccggcggcg ctcgacgggg
      181 tgctgtcccc cgaaggcctc gcccggatcg ccgaggagcg cgagctcacc ccgacccccg
      241 tcggcgacga cgtggcggtc ggcgcgtacg tcggcgaccg gctcggccgc gaggtcgtcg
      301 accggctggt ggagccgctg ctcggcgggg tctacgcggg cgacgcctac cggatctcga
      361 tgcgggccgc cgtaccgcag ctcttcgaag cggtgaagga aggcggcccg ctgctggagg
      421 ccgtccgccg catccaggag cgagcagcgg cgcggcagca gaccgggccc gtcttccagg
      481 gcatcgcggg cggcatcggc accctgccgg acgccgtggg cgacgccgta cgggccgggg
      541 gcggcgcgat cctcacggag accccggtcc tcggtctgac ccgtacggac gcgggctggg
      601 cggtccgcac cgacaccggg gtgatcaccg ccgacggcat cgtcctcgcc acccccgcct
      661 ggtccgcctc cgccctgctc gccgccgagt ccccggccgc ctccgccgag ctgaccggcg
      721 tcgagtacgc ctcgatggcc ctggtcaccc tggccttccg ccgtaccgac atcgaggcgt
      781 acgaggccct gcgcggccgc tccggcttcc tcgtaccgcc ggtcgacggc cgcaccatca
      841 aggcctccac cttctccagc aacaagtggc agtgggtcgc cgacgccgcc cccgacctct
      901 tcgtgctccg cacctcggtg ggccggtacg gcgaggagga ccacctgcac cacgaggacg
      961 gcgaactcgt cgccgtgtcc ctgcgcgacc tccgggcggc caccggactc gccgcccgcc
     1021 ccgtcgacag cgaggtcacc cgctggatcg gcgggctgcc gcagtacccg gtcggccacc
     1081 acacccgggt cgcccgcatc cgcgacgagg tggcgaagct gcccgccctg cgggtctgcg
     1141 gcgcggtgta cgacggggtc ggcatcccgg cgtgcatcgc gagcgcacgg caggcagccg
     1201 acgagatcgc gggggagatc accgggaaga tcgcggggga gatcatcgcc acgcccaccc
     1261 tggttcaggg caccgggagc gaggcgggac aatagccgta tgagtgctcc tgagactgtg
     1321 acatccagca agagtccgaa cgccggaaag aaggccaagg acctcaacga ggtcatccgc
     1381 tacaccctct ggtcggtctt caaactgcgc gacgtcctgc cgctggaccg ggcggggtac
     1441 gcggacgagg tccaggagct gttcgaccag ctcgcggcca aggacatcac cgtgcgcggg
     1501 acctacgacg tctcgggtct gcgcgcggac gcggacctga tgatctggtg gcacgcggag
     1561 acctcggacg agctccagga ggcctacaac ctcttccgcc gcacgaagct cgggcgcgct
     1621 ctggaaccgg tctggtcgaa catggccctg caccggcccg ccgagttcaa caagtcgcac
     1681 gtcccggcgt tcctcgccga cgagacgccc cgcgactaca tcagcgtcta ccccttcgtc
     1741 cggtcctacg actggtacct gctgcccgac gaggaccgcc gccgcatgct cgcggaccac
     1801 ggcaagatgg cccggggcta ccccgacgtc cgcgccaaca ccgtcgcctc gttctcgctc
     1861 ggcgactacg agtgggtcct cgcgttcgag gcggacgagc tgtaccgcat cgtcgacctg
     1921 atgcgccacc tgcgcgcctc cgaggcacgg ctccacgtgc gtgaggaggt cccgttctac
     1981 acgggccgca ggaagagcgt cgcagacctg gtggccgggc tcgcatagcc agcgagttcc
     2041 cgcaccaccc caacccggaa gatcagaaga cgggcaccag gcaccgaccg tgccgcccgc
     2101 cgttccagcg acaccgggtt cggcttgagg ccctgccgga gtgcacttac cgcactccgg
     2161 cagggccctt ccgcgttcct ggctcagtta cttcgctagt ccagcgagac cgggttcggc
     2221 tccgcgcgcg gggcgcactc cgcgtccccg tccggtgtcg cgcccgtcag caggtagcgc
     2281 cgcaggtgct cgtccacgca ggtgttcccg gccccgccga cgccgtgcgt ccccgcgtcc
     2341 cgctcggtca ccagcaccgc gccgcgcagc cgccgctgga gctccagcgc ccccttgtac
     2401 ggggtcgccg catcgcgttc cgccgccagg atcagcaccg gcggtagctc gccccacccg
     2461 gtggagacgt ccaacggctg ctgccggggc gcgggccaga aggcgcacgg caggttggtg
     2521 aaggcgttgt cccaggtctg gaacggggcg atacgggcga ggtcggtgtg gtcgcggtcc
     2581 cagacctccc actcctcggg ccacggcgcg tcgttgcact cgaccgcggt gtagacggcc
     2641 cgggcgttct cgtcgtcctt cgccgtgatc gcgcgcggcg acatctgggt gaccagcggc
     2701 tccgggttgc cccggacgta ctcggagagc gcggtggccc gcatcgccca gtagtcgtcg
     2761 tagtacgccg cctcccggaa cgccgcgtgg aactgccccg gccccacctt cccgcccgcc
     2821 ggctccgtgg cgagggcggt gcggacgtcc tcgtaatggc cctgcacggc ctcgggcgtg
     2881 gggccgagcc cgtacacgtc gtcgtgcttg gcgacccacc gccggaagtc ctcccagcgg
     2941 gactcgaagg cgagtgactg gtcgaggttg gagcggtacc agatctgctc cgggtccggg
     3001 tcgaccgcgg aatcgaagac catacggcgt acgcgtgagg gaaagagggt cgcgtacagg
     3061 gaacccaggt acgtgccgta cgaggcaccc atgaaggtga ggcgttcgtc cccgagggcg
     3121 gcgcgcagca cctccaggtc acgggcgttg ttgagcgagg tgtagtggcg cagcgcctca
     3181 cccgcgtgct tctcgcaccc ccgcgcgtac gcccgcgcct gcgcgatccg ctgctccttg
     3241 tacgcggtcg tggggtgggc gggggagtcg gtggggctct tggcgaaggc ggcgggattc
     3301 tggcaggaga gcggggcgga ggcgccgaca ccgcgcgggg cgtagccgac catgtcgtac
     3361 gcctcggcga ccgccttcca ctcggacatc tcgcccatca gcgggaaggt gatgctggag
     3421 gcgccggggc cgcccgggtt gtagacgaag gatccctgcc ggccggccgc aggtccggtg
     3481 gcgagggtgc ggctgacggt gaggtcgatg gtccgcccgt ccggccgcgc gtagtccagc
     3541 gggacgctga cggtgccgca cttcagggtg tccggcagca tctcctcctc cgggcacgcg
     3601 ccgaaggaga tcccggcggc cgaggcgcgg gccgccgcca ccgccgtacc gcgctcctcg
     3661 gcggaggccg ccggtccggg gccgccgtcg gcggtggcgg ggacggcggc cagggcggac
     3721 aggaccaggg agccgatcgt tgcgtacagc ggtactgctc tcacggcggg acctctctgc
     3781 ggatgcggag aggggaggat ggggtgcggg gcgggtgaag tacagcaccg ggccggtgtg
     3841 ttgcggggaa cgacccggtt gccgcacttc ggtcgccgct cgtggctcag gagcccttac
     3901 gggtcgtggc ccaggagccg ctgtggggca tggctcagga gccttacggt ccgtggctca
     3961 ggagctgctg ccacagctgg acccgccgcc gcagctggag ccgcccgagc tgcccccgca
     4021 gcttgatccc gagcctccgc agctggagcc ggagccaccg cacccggagc ctccgccgca
     4081 gccccctccg cccccgccac cgcaactcgt gtgtccgggg cccgcgtcgg cgcaccacac
     4141 cgtggtcgcc gcgaaggccg acgcccccgc cgtaccgccc acgtaccccg tcgaccggcg
     4201 ccccgaggag ccgggccgcg accggcgggt ccgcgtgtgc agccgtgccg ccgcgatcag
     4261 ctgggcctgg agcacggggt cggggagggc gcgcagcccc cggttggcga cgaggtacgg
     4321 cggggtcagc acggacgcgt tcgcggtccg gaacgcctgc gcggcccggc ggcccgcccg
     4381 ggtgatccgg ttggcggcgg acgcggccac gacgaacccg ccgatcccgc cgagcaggat
     4441 cgcgggcagc atcttcagga cgaacgggac cggcatgtcg aacgagtcct cgagcgacgt
     4501 gtactggatg accgtcagga tgatggagag cgggaacgcg acgaagcagc tcacgccctg
     4561 gatcagcgcc caccgccgta cgggacggct ctcggcgggc cggacgagca gcccgcgctg
     4621 ggccagcccg tcgccgatct cctgcaccgc cgggtggtgc atcacggcct cccgtaccgc
     4681 gtgcagcgcc ccgctcggcg cggccgacag cgcctggagc acggcgcgtt ccaccggatc
     4741 ctgcgcgtgc gcacgctgta cggagatgat gccggggccg ccgatgagca cccggccgtc
     4801 cgcgtacagc gcggtgagcg cggtgtccgt gaccctggcc gggccgccgt tcaggaacgc
     4861 cgcctcgtag aggtcgtgga cggtggggga ggggccggcc gaggggcggc gggaccggga
     4921 gacgaccacc ttcgccagga gcagcacggt ggagagcgcg accgtgaggg tcagtgccag
     4981 agcgagcgtg tccatggcgg tcacttccct gcgagggcgg cgcgcgccgc ccggacgata
     5041 cgggtggtcc ggctcggcgg gcggggcccg gagcggtcct cccaccagtg ggtcagccgg
     5101 cgctgcgcca cggggtcggg gaggccgccg tggaccagga tccgctccac gaagtcgagg
     5161 gcgtcgcgcc ggtagccgtc gctcatcgga cgggtcctgg cgtagtcgag gaaggcggcg
     5221 cggtagccgt cgccgaggat ctccggcagc tcgggcgcca ccttggcgac gacgtcggcg
     5281 cgcttggcgg ccagcgcccg gctctgcacg cgcaaccgcc ggtggtcgaa gccctcgggc
     5341 gccggggtgc ccgcgacgag cgcggagagc agggcggtct gggcgacggc ggtacggtca
     5401 cgggctccgg ggcgcgcggg ggcgggcggg gcggtcgtcc ccgtccaggt ccccgtacgg
     5461 accccgctcg ggccgcccgc cgcccgcagc gttccccgga tcgtctccag ctcggccgcc
     5521 agctccgggc cgggcgggaa ggcgtcgtcg cgctccagca ggacgcccgg cgggtcgacc
     5581 cgggagcgca gctcggccag cacgtccagc accggggcgg tgaccgggtg ggcgtgggtg
     5641 tcgtgccaga cgccgttctt ctcgacgccg cccgccacat ggacgtacgc gatcgcctcc
     5701 accggcagct cgtccagcgc cttcgccggg tcctggccga ggttgacgtg gttggtgtgc
     5761 aggttggcca cgtcgatcag cagccgcacc ccggtccgct cgaccagctc cgcgaggaac
     5821 tgcccctccg tcagctcctc ctcgggccag gagaccagcg ccgcgatgtt ctccagggcc
     5881 agcggcacgg gcagcgcgtc ctgggcgatc gccacgttct cgcagagcac ctccagcgcg
     5941 tcccaggtgc gcggcaccgg cagcaggtgg cccgcctcca gcctcggcga cgcggtcagc
     6001 ggccccccgg cccggacgaa cgcgatgtgc tcggtcacca gcggcgtccc cagcagctcg
     6061 gcccgcgccg cgagaccggc gagccgctcg gcgtccgggc ggtccgcgcc gcccaggccg
     6121 agcgacacac cgtgcggtac gacggtcacg ccccgttccc tcaagcgcac cagggagtcc
     6181 gggaggtggt cggcgcagag gttctccgcc accacctcga cccagtcgac ccccgtgagc
     6241 gcctccacct cggccgcgat ctccggccgc cagccgattc cgatgcccag cttcatggtg
     6301 tccccctcct ccgctccgtc ccggggtgat ggccccggcg agcggagtcc aacccgaacc
     6361 gtgggacgtt cagaggagga tttgaggttc cggtgcttcg gtgtcgttac ggcatcggag
     6421 gcatcgtcgg cggcaccggg cgcgaggtga aggactggtc gccggtcggc gtcaccggta
     6481 ccgggaccgg cggcacctgc ccgccctgag gcatggaggg ccccgaggga ctggcggtgg
     6541 acccgccggc cgcctcgcgc gccagggcgt cgaagtcgac gaagccggtg ccctccagca
     6601 tggtgatgtg gtccagcacg gtctggttgg cgtcgctggc gagctgccgg atcagggtgt
     6661 tgcgggtggt gtgccggacc tggcctatca ggccgaagac cttgccgtgc gcggcccgca
     6721 ggatgttggc gaacttccgc tcgtactcgt ccccgctcgc cgccgtcagc tcgttgagcc
     6781 acgcctgctg ctggtcggtc ggctggttgg gcagctcgac gccgagcttc gccgccacgt
     6841 cccgggcccg ccggtccagg tccgtgtggc cgacgaccag gtggtccccg gcggccttcg
     6901 tcgcctcgct gggagcccgt tcgatcgcct gctgcccggc ggggagctcc cacagcccgg
     6961 cgagccgcac cttgaccagg aagtcccggt cggtcgcgga gagcgggccc cactgggtgg
     7021 cgacgctgga ggcgttgaga ttcgcctcgc cggtgcccga gcggtccgcg taggaccaga
     7081 cggggaaggc gagtgcgccc accgtggcga ccagtgccgc gatgatgagg gccgttccgt
     7141 tgatgcgtcg caacaaggtg tctcccggga cgaagcgtga cagcgttgat caggcgtcag
     7201 tcaaccgccg accggcgtgt cccgcaggct acttggcggt ggtgccggtg gggcggcatc
     7261 gggagatacg tcatcgggcg cgggaacgtt cagcttcgac ggggccatgg tcctggcccg
     7321 cccccgggag cctcacttca ttccgacttg acctcaactg cgatcgaggt tcaagaatcc
     7381 cttccggtgc gtccatccgc acctcgtcgt gaacgacccg acaaggagca tgtgttgact
     7441 gaccggaaga tcattctcgt cctcggtggg accggcaacc agggtggcgc gacggcccgc
     7501 gaactgctgt cccggggctg gtcggtgcgc gcactcgtgc gtgaccccga caagcccgag
     7561 gcccaggcgc tcaaggagct gggcgcggtg ctggtgcggg gggatctcga ggacgtcgcc
     7621 tcactgcgcg ccgccgccga gggcgtccac ggcatcttca gtgtgcaggc gctcgcctac
     7681 gagccggaga cgctcgccgc cgaggtacgt cacggcaagg ccgtcgccga cgtggcgaag
     7741 gaggccggcg tcacccactt cgtctacagc tccgtgggcg gcgccgagcg tgggacgggc
     7801 atcgaccact tcgagagcaa ggccgagatc gagcgccaca tcaaggcgct cggcctcccg
     7861 gccaccatcc tgcggcccgt gttcttcatg aacaacctgc tccacttcgc cgacgtcgag
     7921 ggcgagcggg tgatgtcgct gccggtcgag ccggacaagc cgatgcagtt catcgcctcc
     7981 gacgacatcg gtgtcttcgc ggccgacgcc ttcgaccggc cggccgagtt catcggcagg
     8041 gagatcgagc tcgcgggcga cgagatcacc ttcccgcagg tcgccgaggt ctacgagcgg
     8101 atcacgggca cgccctcgcg cctggaggcc acgcccatcg aggagcgcat gttcgcgtgg
     8161 ttcgccgagg agggctacca ggcggacatc ccggccctgc gtgagctgca ccccgggctg
     8221 ctgacgttcg agcagttcct cgcccagcgc ctcggcacgt cctgacccgg ggcagcccgc
     8281 ccctacggcc gctgcgcggg cgggagggaa ccgacacggt ggtgcccttc ggccaccacg
     8341 gtcggcccgg gagcgatctc ggtgaacccc gcgtcgcgca ccaccggcag gccgctcgcc
     8401 gtcagctcac gccagcgctc cggggtcgcg gtggccacgg agagcgggaa gcccgcctcg
     8461 cgccacgcct tgcgctcggt gtcggacagc tcccaccagg cgagctgggc gccgtggccg
     8521 acctgcgcca tcgtcttgcc cgccgacatc tccagctccg ggttgagcca gagcaccggg
     8581 ccctcggggt cgggggcggc gggcggctcg gggtcgtcca ggtcggtgcc cgacacctgg
     8641 agcttggcca gctccttggg ccagccgtcc agggggatcg gcgggtacac ccgtacctcc
     8701 gcgctcccgc ccgtcaccgt gatcccgggc agcgcggccg ccttgcgcca ctccgcgccg
     8761 cgcgcccggc gcaccacctt gcggatgcgg gcgtcctccc agtcccgcat cgccccggcc
     8821 cactcgccct cgcccgtgga ccgctcgtcg gccaggatcg tgagcaccgc gcgggcggcg
     8881 gtgcgcaggg cgtcggtccg ggcgggcggg gccgtcttct ccaggtgcac cacgagtggg
     8941 agcacgtact gcggggccgc gtcccggtcg gtctgaccgt ggtggaaggg gctcggggag
     9001 gtctcacggg cggggctctg ggaggtgtcg tcgctgctca ccgtcccagt ctgccaggcg
     9061 gcgggtgtca cctcccttgg cttgcggggc cggtccgggt gaggatgctc cgcatgaaga
     9121 gcgatctctt ttccagcgag cacatggccc agccggccac cgcccccggc atgaccctgc
     9181 agaactccaa gtcgatcaaa tacgccgtca acggggagat gcacgcccgc cagggctcga
     9241 tgatcgcctt ccgcggcaac ctccagttcg agcgcaaggg ccagggcatc ggcggcatgc
     9301 tcaagcgcgc ggtcaccggc gagggcctcg cgctcatggc cgtacgcggt cagggcgagg
     9361 cgtggttcgc gcacgaggcc gccaactgtt tcatcctgga gatggaccag ggcgaccaga
     9421 tcaccatcaa cggccgcaac gtcctctgtt tcgacgcgac cctctcgtac gagatcaaga
     9481 cggtgaaggg tgccggaatg atcggcggcg gcctcttcaa cagcgtcttc acggggtacg
     9541 gcaagctcgg cctgatgtgc gagggcaccc ccatagtgat cccggtgacg ggcgcccagc
     9601 cggtgtacgt ggacacggac gcggtcgtcg gctggagcca gcagctcacg accaccctgc
     9661 accgctcgca gagcgtcggg tcgatggtgc gcggcggctc gggcgaggcc gtgcagctga
     9721 tgctccaggg cgaggggttc gtcatcgtgc ggcccagcga ggcccggccc gagaagacgg
     9781 ccaactgacg gccacgttac gtaggctcgt ggccatggac gacgcctcgt actgcgcaac
     9841 gcccgcaccc gccgacccgg aagcggacgc gggcccgccg ttcgcggagt gcgtgctgtg
     9901 ccgcgagccg accgagtacc ccgagtcggc caggggcgcc acgctctgcc cggtctgcgc
     9961 ctggcaggag gcggggcgct cggcctgctc cggttaggca gtttcgtttg gatcaacggg
    10021 cggaccggac gagggtaggg gccccggcag cgtgctggtg ggccgagttc gccacccgcg
    10081 gcgaaccggg gtggaaccct cgcgacggcc tcgccggaag cggggagacc gtacggatcc
    10141 ggcgtacggc agggctgccc ttcctggcgc ctcgacatcg ttccctcgag ggcagccaat
    10201 cggccggatt ttggccggga agtgctgttc ggtcgtcgcc gccctcactg ttgtccgcgc
    10261 ggcgacccgt gtgatgacca cgggatattt acatctccac cccgagggtc cgcttgatca
    10321 ccgggcctcc ggctccctgt cgcctcactg atctcttgct ccggcgcgcc tacttgggag
    10381 taaggtgtcg cgctgtgcga gtgagggatc ttgacctctc cttcacagca cgtgcacatc
    10441 atccggcggc cgggggagca gagccgccac ggggaagagt tcaccgctgt gccgtgacat
    10501 cggctcgttg cgcacgcccc cgtatccgta accccgtcgt ctccgaccag gcgacgggac
    10561 gccgggccga cctcacctcc gttccgctca accggacggc tgcctccacc ggccctcatc
    10621 tcgctgcgaa ggacgacccg ttgccaccca tatccacgcg gtccggacgt agaacgcgct
    10681 ggtccgcact gctcactccc atcgtcacct ccgtcaccgc cctggccgtg ctcggcagcc
    10741 tggccgtcca accggaccag ctcgcccgaa atgcctcacc cgccacggag ccggtcggcg
    10801 gcaactggtc cgagcacacc ccggccgagc aactccggca ggaccagtgc ctgatgagcg
    10861 acgtcctgcg gctcggcggc ctggcgatgt ccgacaccgc gcagcgagga ctcggccaga
    10921 cgcccgaaca gctccgcgtc ctggccgacc gcgaccactg ggagaacacg cccctcgcgg
    10981 cggcctacga caaggaccgc accgccgcca agacggaact cgacgcgatc gccgcacttc
    11041 acgacggttg gaagacgccg ctggccgggc tcgacaatcc ggccggcttc accgacgcct
    11101 ccttccactg gccgcccggc accagcaacg acggccgaaa ggacttccac tcacagaccg
    11161 gtctgagcga ttgggtggcg gatcagttct ggcgggacga ggcggacttc tacgaggact
    11221 ccacccccgc ggcggacgac gcgaccatga aggccgtcaa ggaactgggc acggtgctgt
    11281 acggagggga ccccga
//
