LOCUS       NZ_KB891308            10942 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. ScaeMP-e10 B061DRAFT_scaffold_2.3, whole genome
            shotgun sequence.
ACCESSION   NZ_KB891308
VERSION     NZ_KB891308.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. ScaeMP-e10
  ORGANISM  Streptomyces sp. ScaeMP-e10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 992189)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Improved High-Quality Draft of Streptomyces sp. ScaeMP-e10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 992189)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 992189)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. ScaeMP-e10
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            Isolation Site                    :: Gulf of Mexico
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,185
            CDSs (total)                      :: 7,109
            Genes (coding)                    :: 6,996
            CDSs (with protein)               :: 6,996
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 1 (5S, 16S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 23 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 934140
            Orig. end                         :: 945082
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB891308.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405355
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10942
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="11"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10942
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10942
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5942](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5942
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(1700..2503)
                     /locus_tag="B061_RS36865"
     CDS             complement(1700..2503)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /locus_tag="B061_RS36865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_018513470.1"
                     /transl_table=11
                     /translation="MDIKVLGILSVEEGGIPITPMEAQVRQVLALLAAQVDHTVPTVVL
                     VEELWPVDPPQHATDIVERHIRRLRDLISAALSRAALEAPHGSTPGEPRSADTVLASRS
                     GGYRLDSGGGTSDAWTFARAAGAGYRAMETEDFETAAGRLRDALGLWTAEPYAGVLHGP
                     HLRAHAEQLEQSWQRALDQWIATEMQLGRHLTLHADLSLLLSRSRARQPLYEGLMAELR
                     RTGDPFRVLQAYKRLRRGTPQPRVPRPAAAPVRMERSFGLSASPV"
     misc_feature    complement(2513..2527)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.32"
                     /tool="antismash"
     misc_feature    2587..2600
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.37"
                     /tool="antismash"
     misc_feature    complement(2593..2606)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.07"
                     /tool="antismash"
     misc_feature    2611..2620
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.27"
                     /tool="antismash"
     misc_feature    2616..2634
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.84"
                     /tool="antismash"
     gene            2649..3275
                     /locus_tag="B061_RS36870"
     CDS             2649..3275
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133274.1"
                     /locus_tag="B061_RS36870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_018513471.1"
                     /transl_table=11
                     /translation="MKQARSARTRQALIEAAAGEFDRHGYAGSSLAQISKAAGISMGAL
                     TFHFSSKEELAATVRSRGHYATTAVVHEVSAAEAAPVQAVVQLTLALARLLQDEAVVRA
                     AARLTQERPGAAADWDSAWAPTISDWLHAQGAEGSDPVAFSTLATLLVLGTEADMRRRA
                     AAPEAGHSPVRQLSRVWELSLSGAAAPDGAGTGDGVPCRSARGHG"
     misc_feature    2934..2936
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3346..4119)
                     /locus_tag="B061_RS0120900"
     CDS             complement(3346..4119)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-178). E-value: 8.6e-17.
                     Score: 53.6. Matches aSDomain:
                     nrpspksdomains_B061_RS0120900_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644115.1"
                     /locus_tag="B061_RS0120900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_076966616.1"
                     /sec_met_domain="adh_short (E-value: 1.9e-36, bitscore:
                     121.4, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 4.9e-30, bitscore:
                     100.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDTRDLLHGRTVMITGASSGIGAAAARLFAAEGAAVVLMARRKD
                     KLLALAEEIEAGGGRALVAAGDVTAGGDVERAVADAVDAFGRLDGAFNNAGWGSIGTSL
                     HETDDAVFDRVMETNVRGVWNCLKHQIPVMLGQEDGGAIVNTSSIAGLFATGAIAPYVA
                     AKHAVLGLTRAAAAEYGERGIRVNALMVGSTRTELMETAMREVPQLEQAAAARAIQHRL
                     ADPAEVAQAAAFLLSARASFITGSAMAVDGGCSAI"
     aSDomain        complement(3586..4086)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B061_RS0120900_PKS_KR.1"
                     /evalue="8.60E-17"
                     /label="B061_RS0120900_PKS_KR.1"
                     /locus_tag="B061_RS0120900"
                     /protein_end="178"
                     /protein_start="11"
                     /score="53.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TVMITGASSGIGAAAARLFAAEGAAVVLMARRKDKLLALAEEIEA
                     GGGRALVAAGDVTAGGDVERAVADAVDAFGRLDGAFNNAGWGSIGTSLHETDDAVFDRV
                     METNVRGVWNCLKHQIPVMLGQEDGGAIVNTSSIAGLFATGAIAPYVAAKHAVLGLTRA
                     AAAE"
     CDS_motif       complement(3991..4086)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B061_RS0120900_0001"
                     /evalue="7.70E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="B061_RS0120900"
                     /protein_end="43"
                     /protein_start="11"
                     /score="15.4"
                     /tool="antismash"
                     /translation="TVMITGASSGIGAAAARLFAAEGAAVVLMARR"
     misc_feature    4139..4153
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     gene            complement(4250..4876)
                     /locus_tag="B061_RS0120905"
     CDS             complement(4250..4876)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270948.1"
                     /locus_tag="B061_RS0120905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_018513473.1"
                     /transl_table=11
                     /translation="MTRTVKQDRAVRTRAALIRAGAEVFGECGFAGASVTKIADRAGLT
                     LGAVYFHFKSKEELARLIVAEQPKLVVPPQPSQGLQHAVDVTLTWAYQLLDDPVLIAGA
                     RLVMDQDQFVPPEENSHQQWTQILLKDFQVAERRRELRAGADIQAIARLIVSTCTGAQM
                     HAHMESGRADLPHRVEEMWQVLLPGIAVPSAAKKLEFGEDRGRGR"
     gene            5001..5942
                     /locus_tag="B061_RS36875"
     CDS             5001..5942
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="B061_RS36875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_078533176.1"
                     /sec_met_domain="AfsA (E-value: 6.7e-20, bitscore: 66.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALCTVTERHSTTGAPTGGFDQGLARKTVSTEALVTSWRRISDIT
                     QTVTVQWPQRHSFYTVSTRYSPLLVIESLRQALAVLTHTVHDIPLSHRLGWEHIRSTVD
                     PEALRAGPTSPSLELTITHTKVKRRPGGSVHLASQVEATRDGVALAEAELHYTTHPPAI
                     YNRLRGRYADARQAFAQALPLTPPVPPAHVGRSSVRDVVLSPTSTAHQWQLRVDTTHTV
                     LFDHPHDHVPGMVLLEAADQIARAEAQPQAALPVAFDTTFHRYVEFDRPCVLTAEPVEP
                     DAQGRCRVLVSASQSDQVVFRTTVTTEPSAMR"
     gene            complement(6055..7056)
                     /locus_tag="B061_RS0120915"
     CDS             complement(6055..7056)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459797.1"
                     /locus_tag="B061_RS0120915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldo/keto reductase"
                     /protein_id="WP_018513475.1"
                     /transl_table=11
                     /translation="MYLPSADRYSAMPYRRTGRSGLLLPALSLGLWHNFGGDRTPDEQG
                     RILRRAFDLGITHFDLANNYGPPPGSAELTLGRALAGDFAKLRDELVISTKAGYHMWDG
                     PYGEWGSRKYLRSSLDQSLKRLGVEYVDIFYSHRPDPDTPLEETMGALDTAVRQGKALY
                     VGLSNYSAEETRRAAAILKDLGTPLLIHQPRYSMLDRRPEDGLLTALDELGVGSIAYSP
                     LEQGILTDRYLNGVPANSRAAGDSPFLTSDALTPQLVERLRALDAVAKERGQSLAQLAL
                     AWVLRGGRLTSAVVGASSVTQLENSVEAVRNLEFAEEELTRIEELLAEAGTG"
     gene            7168..8124
                     /locus_tag="B061_RS0120920"
     CDS             7168..8124
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612514.1"
                     /locus_tag="B061_RS0120920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_018513476.1"
                     /transl_table=11
                     /translation="MELRQLEHFVAVAEEQHFTRAAERLAVSQSGLSASVRALEQELRT
                     PLFSRTTRTVRLTEAGRALLVEAERTLAGARAARDAVDAVRGLLRGTLTLGVEQCVAGV
                     SPARLLAAFHREHPQMELRLRQEGTSSLLDGVAGGRLDLAFAATVSPAEWRGELMPLAR
                     EEMVVLCAAGHRFAGRARVGWEELPGESFIDFHPDWGPRRAADEAFAAAGARRTVGLEV
                     NDVHSLLELVQEGLGIAVVPHHFSRKPEAARLTAVEVAPAGGRQAVYESVVVLPAARSL
                     SPGARALMRLVREDAVREDAVREAGVRGAEVREAGVG"
     gene            complement(8177..10579)
                     /locus_tag="B061_RS0120925"
     CDS             complement(8177..10579)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612513.1"
                     /locus_tag="B061_RS0120925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_018513477.1"
                     /transl_table=11
                     /translation="MVGGITLVKDEPDRTAAAVGDPPPPADARPHPVAEEISPRRIRMV
                     FLGLMLTLLLAALDQMIVATALPRIVGELHGLEKMSWAVTAYLLASTIVLPLYGKLGDL
                     FGRKGVFQFAIVVFVIGSALAGWSRTMDELIAFRAIQGIGGGGLMIGVQAIIADIVPPR
                     ERGRYMGLIGAVFGLASVAGPLLGGFFTDHASWRWCFYINVPFGLVTFAVIAVVLKLPK
                     PAVRPRLDVLGAVLLAAASTCLVLVTSWGGTEYAWGSRTILGLSAGAVVTTLLFVAVEH
                     RAAEPIIPLRLFRDSIFNITGLVGAVVGIALFGAASYLPTYLQMVDGASATESGLLMLP
                     MMLGIVGGSIVSGQLITRTGRYRVYPIVGSAVSVVGMWLLSGLERGTSRLEYSLWQAVL
                     GTGIGLVMSVLVLAVQNSVRPADLGTATSANNYFRQIGGSVGAAIFGTLFAGRLSDALD
                     LRLPTGVTLPAPESITPQIVHAMEPALRDAYIQAYVDAMPRIFLYLVPVLVLGLILAFF
                     LKEKPLVSHHSPEAATEPSPIPAARTQPSRLSGVPVRGSVQHPDGTKVARAALTLIDVQ
                     GRQVGRGASGEDGGYALSVPGAGGYVLIAAAGGHQPQAVSVTVGDRPVELDVVLGGAGR
                     LAGSVLTADGVPVRDAAVTLTDVRGEVVASTRTGREGGYVISELVAGEYTLAASAPAYR
                     PAALPVSVQAARETRQDIELAGGAVLRGVVRATGGRPVEDARVTLLDAAGNVVDTLTTG
                     PDGSFRFVDLSSGEYTVIAAGYPPVATVLQVAGGGRTERDLQLGHED"
ORIGIN
        1 tcggcgaccg cccgggccgc ggcggaggcg tcgtcgccgg gcaggagcag caggatgcag
       61 ccgtcgtgcg cggtcttgag accggacatc cggtgggcgt aggaggaagc ccagaccagg
      121 gcgcggccgt gctccccgcc ctcggggcgc ccgaccacca ggacatgggg cgcgtcgagg
      181 tcgaggccga tccggtgggc gcgcttggcc aggtgccgca gacggtgcgc ggagcggctg
      241 gacgtggcga gcaggtcgtc gaagagttcg tcgcgtacgg gcccttcggc cagtgccgtg
      301 ctgtgctgga ggaggagctg gatcgcgaag gcctgcgccg tgaggtgcag caggcgttcg
      361 tcctccccta ccagtgcggt ggcggggcgc aggatcagga agcccaggtc ctccgcgccg
      421 gcgatgaccg gggcgaccca gatgttgccg gaggcggcca ccgcggtacg ccgggtgtgg
      481 gcgtccagag cggtcctggc cacggcttcc tcgtcgaggt ccggtatctc cccgccgccg
      541 gccaggaggc ggccggcggg atcgcgtagc tggagggtgc cgtccagcgc gtccccggcc
      601 gccttggcca cggtggtgag gtcggctccg gcgagcagca ggctcatggt gcggctgtgg
      661 gcctcgttga gctgctggag tctgaccagg ctggtgcggg tgcgcagacc gtccagttcg
      721 agctcgtcga cctcgccccg ggcctgctcg agccgctcgg cccgttcgat ggccacggcg
      781 gcgagcatgg ccagcgagca gaccagcccg atctcgtcgg ggctgaagtg gcgtaccgcg
      841 cgggtggcgc cgtacagcac gccgacgggg ctgttcccgt tcagcagcgg tacggccatg
      901 agggcgcgca gcccctcggc gcgcacgaca tcggtctcct cgcaggcctc gatgccttcg
      961 ccgccgggat agtcggccgt ccacacgggg gcgccggtgg tcagcgcccg gtggccgaga
     1021 ccacggccgg gggcgatacg gaactcgttc gcgagggcgg tggtcgaccc ttccgacgta
     1081 cgggcgtaca gcgtgccgtc cgcgtccatc agcgcgacgc aggtcatgtc gaagttgagg
     1141 agccgcttga ccctccgggt gacgacccgc agcaacgcgt cgagttcata ggggaaggtc
     1201 agatcccggg cggtgtcgac caggtcggcg aggccgtcct cccgctggcg gcgccggccc
     1261 tgtaaggcgt ggacgccgcg ggccagcgag acggcgcgtt cgaggcgggc gatgtcgtcc
     1321 ggcgcttgac cggcccggcg agcgctctgc agaagctcct cgaaccggct ggggggagcc
     1381 tcctcggcga ggagttccag gacggtgagc agatccgctc cgcccgtccg cgtgttcgtc
     1441 gtcacgtgcc gatccccccg gtggccccag tccccttacc tggccgaaac taattcgccg
     1501 atgaactgcc agtcaatcgg ttaccggcgg tgatggcgtc gggtggtggc cgggccacca
     1561 cggcgggagg ccgaaaggtg gctggagacg gtacccggcc gcggtgggtc tgtggtcggg
     1621 gtgaggaaca tgccccagag acaggacgtc cggacgccga ggcgtccgga cgtccttgac
     1681 atgtgccggg ccggagtcgt cagacggggc tggcgctcag cccgaacgac cgctccatcc
     1741 ggacgggtgc cgccgccggg cggggcaccc gcggctgcgg cgtgccccgg cgcagccgct
     1801 tgtaggcctg gagcacgcgg aaggggtcgc cggtgcggcg cagttccgcc atgagccctt
     1861 cgtacagcgg ctgacgggcg cgggaccggg acaggagcag cgacaggtcg gcgtgcaggg
     1921 tgaggtgccg gccgagctgc atctcggtgg cgatccactg gtcgagggcg cgctgccagg
     1981 actgttcgag ctgctcggcg tgggcgcgca ggtgcgggcc gtgcaggaca ccggcgtacg
     2041 gctcggcggt ccacaggccc agcgcgtcgc ggagccggcc ggccgcggtc tcgaagtcct
     2101 cggtctccat ggcccggtag ccggcgcccg ccgcccgggc gaacgtccag gcgtcgctcg
     2161 taccgccgcc gctgtcgagg cggtagccgc ccgaccggga ggccagcacg gtgtctgcgc
     2221 tgcgcggctc gccgggggtg gagccgtggg gcgcctccag cgccgcccgg gagagggcgg
     2281 cggaaatgag gtcccgcagc cggcggatgt ggcgttccac gatgtcggtg gcgtgctggg
     2341 gcgggtcgac gggccagagc tcctcgacga ggacgaccgt cggaaccgtg tggtcgacct
     2401 gggccgccag cagcgcgagg acctgccgga cctgcgcctc catcggtgtg atcggtatgc
     2461 ctccctcttc cacggacagg atccccagca ccttgatgtc cattacgccc cctgtaaatg
     2521 accgtcaggg cgtacggcag tgcacgccgt tccccacggc gctggtgcac tcccctggcc
     2581 ccccactaca gttaataata gtggtttttt tgttttttgg tgaggctcaa ctgccgcgaa
     2641 ggggaactgt gaaacaggca aggagcgcgc ggacgcgcca ggctctgatc gaggcggcgg
     2701 ccggggagtt cgaccgtcac gggtacgcgg ggtcctcgct cgcccagatc agcaaggcgg
     2761 ccggcatctc catgggcgcg ctgacgttcc acttctcgtc gaaggaggag ctggcggcga
     2821 ccgtcaggag ccgggggcac tacgccacca ccgcggtggt gcacgaggtg tccgccgccg
     2881 aggccgcccc cgtgcaggcc gtggtgcaac tcactctggc actggccagg ctgttacagg
     2941 acgaggcggt cgtacgggcc gccgcacgcc tgacgcagga gcggccgggt gccgccgcgg
     3001 actgggacag cgcatgggct ccgacgatca gcgactggct gcacgcgcag ggcgcggagg
     3061 gctccgaccc cgtggcgttc tcgaccctcg ccaccctgct ggtgctgggc acggaggcgg
     3121 acatgcggcg gcgcgcggcc gctcccgagg ccgggcacag cccggtccga cagctctccc
     3181 gggtatggga gttgtccctg tccggcgcgg ccgcgccgga cggcgcgggg accggtgacg
     3241 gtgtgccgtg ccggtccgcg cgcgggcacg gctgagcgcg gcaagccgcc ggaacgtgtt
     3301 gtgccgccgt cgtgtgacgg cggcacaaca cgtcttccgt gaggctcaga tggcgctgca
     3361 gcctccgtcg acggccatgg cgctgccggt gatgaaggag gcgcgtgcgc tgaggaggaa
     3421 ggcggccgcc tgggcgactt cggcggggtc ggccagccgg tgctggatgg cgcgcgccgc
     3481 ggccgcctgc tccagctgcg gcacctcccg catcgccgtc tccatcagct cggtgcgcgt
     3541 gctccccacc atgagggcgt tcactctgat gccgcgctct ccgtactccg ccgccgcggc
     3601 ccgggtcagg cctaggacgg catgcttggc cgcgacatag ggagcgatcg ccccggtggc
     3661 gaacaggccc gcgatgctcg acgtgttcac gatcgcaccg ccgtcctcct gccccagcat
     3721 caccggtatc tggtgcttga ggcagttcca cacaccgcgg acgttcgtct ccatgacgcg
     3781 gtcgaagacc gcgtcatccg tctcgtgcag cgaggtgccg atcgagcccc atccggcgtt
     3841 gttgaaggcc ccgtcgagcc ggccgaaagc gtccaccgcg tcggcgacgg cccgctcgac
     3901 gtcaccgccc gccgtgacgt cgccggccgc caccagcgcc cggccgccac cggcctcgat
     3961 ctcctcggcg agcgccagca gcttgtcctt gcggcgcgcc atcagcacca ccgccgcacc
     4021 ctcggcggcg aaaagccggg cggctgccgc acctatgccg ctcgaagccc ccgtgatcat
     4081 gacagtcctg ccgtggagca ggtcccgcgt atctcccatt gacgccccct cgttctccat
     4141 caaccccccc gatcacgtag ttgatcagga catacgtggg acaccgtagc cgtgccgtat
     4201 gacgggccgg ccggtcagca ccggctgcgg cccgtaccgg cgtacggcgt caccggcctc
     4261 tcccccggtc ctcgccgaac tccagcttct tcgcggcgct cggcacggcg atgccgggga
     4321 gcagaacctg ccacatctcc tcgacgcggt gcggaaggtc cgcgcgcccc gactccatgt
     4381 gggcgtgcat ctgcgcgccc gtacaggtgc tgacgatgag acgggcgatc gcctggatgt
     4441 cggctccggc ccgcagttca cgcctgcgct cggcgacctg gaagtccttg agcagtatct
     4501 gcgtccactg ctggtgggag ttctcctccg gcgggacgaa ctggtcctgg tccatcacca
     4561 gccgcgctcc ggcgatcagc accgggtcgt ccagcagttg gtaggcccag gtcagggtca
     4621 cgtcgacagc gtgctgcaat ccctgcgagg gctgcggcgg aacgacgagc ttcggctgct
     4681 cggcgacgat cagccgggcc agctcctcct tggacttgaa gtggaagtac acggcgccca
     4741 gggtcagtcc ggcccggtcg gcgatcttcg tgacgctggc tccggcgaac ccgcactctc
     4801 cgaagacttc cgcacccgcg cgaatgaggg ctgcacgggt acgtaccgct ctgtcctgct
     4861 tcacggtccg cgtcacttgt gcctgctccc tcaaactcgg tctcgacaag aaaaaaagta
     4921 ttctctactt tatagctcgc gggtgatgcg gccgcacacc tcctgcagtc accacgaagt
     4981 tccgggggag agaactcgtc atggcactct gcacagtcac cgagagacac agcacgaccg
     5041 gcgcaccgac cggggggttc gaccaagggc tggcgcgcaa gacggtctcc acggaggctc
     5101 tcgtcacctc gtggcggagg atctccgaca ttactcagac ggtcaccgta cagtggcccc
     5161 agcggcacag tttctacaca gtatctacgc gctacagccc cctgttggtc atcgagagcc
     5221 tgcgccaggc gctggccgtg ctgacccaca cggtgcacga catcccgctc agccaccggc
     5281 tcggctggga gcacatacgc tccaccgtcg accccgaggc cctccgggcc ggtccgacgt
     5341 ccccgtcctt ggaactcacc atcacccaca cgaaggtgaa gcggcgcccc ggcggctccg
     5401 tccatctggc ctcccaggtc gaggccaccc gcgacggcgt cgccctggca gaggccgagc
     5461 tgcactacac cacgcatccg cccgccatct acaaccgtct gcgggggcgc tacgcggacg
     5521 cccgacaggc cttcgcccag gcgctccccc tcacaccgcc ggtgccgccc gcccacgtgg
     5581 ggcgcagcag tgtgcgggat gtggtgctgt ccccgacgag caccgcccat caatggcagc
     5641 tgcgggtcga caccacgcac accgtgctgt tcgaccaccc gcacgaccac gtccccggga
     5701 tggtgctgct ggaggcggcc gaccagatcg cacgcgccga ggcccagccg caggccgcgc
     5761 tcccggtggc cttcgacacc accttccacc gctacgtcga gttcgaccgg ccctgcgtcc
     5821 tcacggccga gcccgtcgag cccgacgccc agggccgttg ccgcgtcctg gtcagcgctt
     5881 cccagtccga ccaggtggtc ttccggacca ctgtgaccac cgagcccagc gccatgcgct
     5941 agacacacga cggcaccacc cggagcagcc gcttgcccgc cggcgatccc ggcgggcaag
     6001 cggctgtcac catgcacacg atgcgcgtgt gccgtggccg tcggccggtg ggcgtcagcc
     6061 cgtcccggcc tcggccagca gctcctcgat ccgggtcagc tcctcctcgg cgaactccag
     6121 gttccgcacg gcctccacgc tgttctccag ctgggtgacg ctgctcgcgc cgaccacggc
     6181 cgaggtgagg cggccgccgc gcagcaccca tgccagggcc agctgggcga gggactgccc
     6241 gcgctccttc gccacggcgt ccagagcccg cagccgctcc accagctggg gcgtcagggc
     6301 gtcggacgtc aggaacgggc tgtcgcccgc cgcccgggag ttggccggga cgccgttcag
     6361 gtagcggtcg gtgaggatgc cctgctccag cggggagtag gcgatggagc cgacgcccag
     6421 ctcgtccagc gcggtgagca gcccgtcctc ggggcgacgg tccagcatcg agtagcgggg
     6481 ctggtggatg agcaggggcg taccgaggtc cttgaggatc gcggcggccc ggcgggtctc
     6541 ctcggcggag tagttggaga ggccgacgta gagggccttg ccctggcgta cggcggtgtc
     6601 gagcgcgccc atcgtctctt cgagcggggt gtcggggtcg gggcggtggg agtagaagat
     6661 gtcgacatac tcgacgccca ggcgcttgag gctctggtcc agcgaggagc gcagatactt
     6721 ccgcgagccc cactccccgt acgggccgtc ccacatgtgg tagccggcct tggtggagat
     6781 gacgagctcg tcgcgcagct tcgcgaagtc accggcgagc gcgcggccca gggtgagttc
     6841 ggcggagccg ggcggcgggc cgtagttgtt ggccaggtcg aagtgggtga tgccgaggtc
     6901 gaaggcgcgg cgcaggatgc ggccctgctc gtcgggggta cggtccccgc cgaagttgtg
     6961 ccagagcccg agggagagcg cgggcagcag gagaccgctt cggccggtgc gccggtaggg
     7021 catggcggag tagcggtcgg cggacggcag gtacatacga gagctccgcg gggagagggc
     7081 cgggggacgg gccttaaggc ttccgcagat cgatccagca gtccaacagg agattccgct
     7141 cggattcatc acctagagtc ctcaatcatg gaactgcgtc agctggagca cttcgtcgcc
     7201 gtcgccgagg agcagcactt cacccgcgcc gccgagcgcc tcgccgtctc ccagtccggg
     7261 ctctccgcct ccgtacgggc cctggagcag gagctgcgca ccccgctgtt ctcccgcacc
     7321 acacggacgg tccggctcac cgaggcgggg cgggcgctgc tggtggaggc ggagcgcacg
     7381 ctggcggggg cgcgggcggc gcgggacgcg gtcgatgccg tacgggggct gctgcgcggg
     7441 acgctgaccc tgggcgtcga acagtgcgtg gcgggggtga gcccggcccg gctgctggcc
     7501 gcgttccacc gggagcaccc gcagatggag ctgcggctgc gccaggaggg gacgagcagt
     7561 ctgctggacg gggtggccgg agggcgcctg gacctggcgt tcgccgccac ggtcagcccg
     7621 gcggagtggc gcggggagct gatgccgctg gcccgggagg agatggtggt gctgtgcgcg
     7681 gcgggccacc ggttcgcggg ccgggcccgg gtggggtggg aggagctgcc cggtgagtcg
     7741 ttcatcgact tccacccgga ctgggggccg cggcgtgcgg cggacgaggc gttcgcggcg
     7801 gcgggggccc ggcggacggt gggcctggag gtcaacgacg tgcacagcct gctggagctg
     7861 gtgcaggagg ggctggggat cgcggtggtg ccgcaccact tctcccgcaa gccggaggcg
     7921 gcccggctga ccgcggtgga ggtggcgcct gcgggcgggc ggcaggcggt gtacgagagc
     7981 gtggtggtgc ttccggcggc gcggagcctg agcccggggg cgcgggcgct gatgcggctg
     8041 gtgcgggagg acgcggtacg ggaggacgcg gtacgggaag cgggggtgcg gggagcggag
     8101 gtgcgggagg cgggggtggg ctgagccggt caggagcccg taccggactc gtgggctcag
     8161 ctcacccccg tacgcctcag tcctcgtggc ccagttggag gtcccgttcc gtgcggccgc
     8221 cgcccgccac ctggagcacc gtcgcgaccg gcgggtatcc ggcggcgatc accgtgtact
     8281 cccccgacga caggtccacg aaccggaacg agccgtcggg cccggtggtg agggtgtcca
     8341 ccacgttgcc cgccgcgtcc agcagggtga cccgggcgtc ctcgaccggc cggcccccgg
     8401 tggcccggac gacgccgcgc agcacggccc cgcccgccag ctcgatgtcc tgccgggtct
     8461 cgcgggccgc ctggacgctg accgggaggg cggcggggcg gtaggcgggg gcgctggcgg
     8521 cgagggtgta ctcacccgcg accagctcgc tgatgacgta gccgccctcg cgcccggtgc
     8581 gggtggaggc gacgacctcg ccgcgtacgt cggtgagggt gacggccgcg tcccgcacgg
     8641 gcaccccgtc ggcggtcagg acggacccgg cgagccgtcc ggcgccgccg aggaccacgt
     8701 ccagctccac ggggcggtcg cccacggtga cgctgacggc ctgcggctgg tggccgcccg
     8761 ccgcggcgat gaggacgtac cctccggcgc ccgggacgct cagcgcgtac cccccgtcct
     8821 cgccgctggc cccgcgcccg acctgccgcc cctggacgtc gatgagcgtg agggcggccc
     8881 gcgccacctt cgtaccgtcc gggtgctgga cgctgccgcg cacgggcaca ccggagagcc
     8941 tggaaggctg ggtgcgggcg gcggggatcg gtgagggctc ggtggccgct tcggggctgt
     9001 ggtgggacac cagcggtttc tccttgagga agaaggcgag gatcaggccg aggacgagca
     9061 ccggcacgag gtagaggaag atgcgcggca tcgcgtcgac gtacgcctgg atgtaggcgt
     9121 cacgcagcgc gggctccatc gcgtggacga tctgcggggt gatcgactcg ggcgcgggca
     9181 gcgtgacacc cgtgggcagc cggaggtcga gggcgtcgga gagccgcccg gcgaacagcg
     9241 tgccgaagat ggcggcgccg acgctgccgc cgatctgccg gaagtagttg ttggcgctgg
     9301 tggcggtgcc gaggtcggcg gggcgtacgg agttctgcac ggcgagcacg agaaccgaca
     9361 tgaccaggcc gatgccggtg ccgaggacgg cctgccagag gctgtactcc agccgggagg
     9421 tgccccgctc aagacccgag agcagccaca tgccgacgac ggagacggcg ctgccgacga
     9481 tggggtagac gcggtagcgg ccggtacggg tgatgagctg gccggagacg atggagccgc
     9541 cgacgatgcc cagcatcatc ggaagcatca gcagcccgga ctcggtggcg ctggccccgt
     9601 ccaccatctg gagataggtg ggcagatagc tggcggcgcc gaagagcgcg atgccgacga
     9661 ccgcgccgac gagaccggtg atgttgaaga tcgagtcgcg gaacagccgc aaggggatga
     9721 tgggttcggc cgcgcggtgc tcgacggcga cgaagagcag cgtcgtgacg acggcccccg
     9781 ccgacaggcc caggatggtg cgcgagcccc aggcgtactc cgttccgccc caactggtca
     9841 ccaggaccag gcaggtggag gcggcggcca gcaacacggc gcccaggacg tcgagtcggg
     9901 gccgtacggc gggcttcgga agtttcagca ccaccgcgat cacggcgaac gtgaccagcc
     9961 cgaacggcac gttgatgtag aagcaccagc gccaggaggc gtggtcggtg aagaagccgc
    10021 cgagcagcgg tccggccacc gaggcaagac cgaagaccgc gccgatgagg cccatgtagc
    10081 ggccgcgctc ccggggcggg acgatgtccg cgatgatcgc ctggacgccg atcatgagac
    10141 cgccgccgcc gatgccctgg atggcgcgga aggcgatcag ctcgtccatg gtccgcgacc
    10201 agccggccag cgcggagccg atgacgaaga cgacgatggc gaactggaag acgcccttgc
    10261 ggccgaagag gtcgccgagc ttcccgtaga gcggcagcac gatcgtggag gcgaggaggt
    10321 aggcggtgac cgcccaggac atcttctcca ggccgtgcag ctcaccgacg atcctgggca
    10381 gggcggtggc gacgatcatc tggtcgagcg ccgcgagcag cagggtgagc atcagcccga
    10441 ggaagaccat gcggatccgg cgggggctga tctcctcggc caccgggtgc ggtcgggcgt
    10501 cggccggtgg tggcggatcc cctacggcgg ccgccgtccg gtccggctcg tccttcacca
    10561 gcgtgatacc gcccaccacg taacgctccc cttcgtcgca cctgcgccgc cctttctcgc
    10621 atcgcgtggc aaggcggggc aaacgggatg aggggggcgc agggcgcgta cgaagggctt
    10681 atgcgccggt cggagccgct acggcgcaca accggccgac ccggaaaacc acccgttccg
    10741 gtgaggccgg ccgaccggcg ggagccggac cgttcgacgg cccggcttcc gtgcgggacc
    10801 tacttctcga cctcggcggc gaggttctgg agcagctcgt cgtagatccg gccgaggccc
    10861 ttgggggcga aggtgcgctc gaagaaaccg ccgatgccgc cggcaccgtc ccagacggtg
    10921 gtgacgacgg ccttggacct gc
//
