LOCUS       NZ_KB891308            10798 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. ScaeMP-e10 B061DRAFT_scaffold_2.3, whole genome
            shotgun sequence.
ACCESSION   NZ_KB891308
VERSION     NZ_KB891308.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. ScaeMP-e10
  ORGANISM  Streptomyces sp. ScaeMP-e10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 992189)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Improved High-Quality Draft of Streptomyces sp. ScaeMP-e10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 992189)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 992189)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. ScaeMP-e10
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            Isolation Site                    :: Gulf of Mexico
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,185
            CDSs (total)                      :: 7,109
            Genes (coding)                    :: 6,996
            CDSs (with protein)               :: 6,996
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 1 (5S, 16S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 23 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 524519
            Orig. end                         :: 535317
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB891308.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405355
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5798
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(501..515)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     misc_feature    complement(665..681)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.35"
                     /tool="antismash"
     gene            complement(<1082..>1303)
                     /locus_tag="B061_RS0119130"
                     /pseudo=""
     CDS             complement(<1082..1303)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964051.1"
                     /locus_tag="B061_RS0119130"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKRAEMGTIAGTHSDLCVLTSDNPRYEDPEAILDQIAPGIAATGT
                     PFERFTDRRAAVASALASAGPADIVLIAG"
     gene            1715..2293
                     /locus_tag="B061_RS0119135"
     CDS             1715..2293
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766037.1"
                     /locus_tag="B061_RS0119135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_018513126.1"
                     /transl_table=11
                     /translation="MSDTDWGDDVYQPQEQEAADPDEQLGSEDTLLDPSGTGDPLDEGY
                     SPPDRPWVVEGLGTTAAESLTGESLETRLTREVPETPGFTGDGVGDLAGGDGELWDGKV
                     GVARAGRLTQQADGTVPATLTASDVGIDGAAASAEEAAMHVIPEEGDDESLLDGSLPDG
                     ALVDGAAEDGAPVDSSPVEGSVKGSAEGT"
     gene            2358..3800
                     /gene="gndA"
                     /locus_tag="B061_RS0119140"
     CDS             2358..3800
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612845.1"
                     /locus_tag="B061_RS0119140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_018513127.1"
                     /transl_table=11
                     /translation="MSGTAQIGVTGLAVMGRNLARNFARNGFTVAVHNRTASRTDALVK
                     EFGDEGTFVAAHTPEEFVTALERPRRLVIMVKAGDPTDAVIQEFAPLLEDGDVIIDGGN
                     AHFEDTRRRERELRERGIHFVGAGVSGGEEGALHGPSIMPGGSAESYASLGPMLEKIAA
                     KAKDGTPCTAHIGPDGAGHFVKMVHNGIEYADMQLIAEAYDLLRRIAGYSPAQIADTFR
                     SWNTGRLDSYLIEITAEVLAHTDAATGKPFVDVVQDRAEQKGTGRWTVQIALDLGVPVS
                     GIAEAVFARSLSGHTDLREASRELPGPVAKPLEGADASAFADRVEQALYASKVVSYTQG
                     FHQIRAGSEAYDWDIDLGSVAAIWRAGCIIRAAFLDRIRAAYDSRPELVSLLSDEEFGR
                     EIGAAQDDWRAVVAEAARQGVPTPGFAAALSYYDGLRAERLPAALTQGQRDFFGAHTYR
                     RTDREGAFHTLWGGDRSEVEAG"
     gene            3940..5001
                     /locus_tag="B061_RS0119145"
     CDS             3940..5001
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="B061_RS0119145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_018513128.1"
                     /transl_table=11
                     /translation="MSAAVPDPVSVQPVVAPLTNAALFLVGTVEPGGEDAVREVLPDLA
                     AVARSLGFRYPDAGLACVTGFGSAVWDRLFGGGRPAHLHPFPELRGRLHHAPATPGDVL
                     LHIRAERMDVCFEWAAQLLTKLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     SAALVGADDDPGFEGGSYVVVQKYLHDLTSWNALSVEEQERVIGRTKLDDVEFPDDEKP
                     ADSHLALNTITDPDGTERDILRANMPFGNFGQGEFGTYFIGYAADPDVTERMLRNMFLG
                     DPPGTHDRILDFSTAVTGGLFFAPRAEFLDDPPPPPSPVPAVDRPEPVPVAAAPDGPEH
                     GSLRIGSLQESAQ"
     gene            5001..5798
                     /locus_tag="B061_RS0119150"
     CDS             5001..5798
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="B061_RS0119150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_018513129.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.5e-88, bitscore:
                     291.8, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPAAWDEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLAAVGDGHLRDIDPPTPDVVARARTSMPVIEWRVPFTVSRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAVIDGYAAAGITGLRDGSSHDPLPLPADARDYPVAVSQAVTRLRLA
                     GVEGPYRLLLGADAFTEAAEMSDHGFPVKTHLARIVDDEILWAPAVKGGVLLSTRGGDF
                     ELCLGQDLSIGYADHDATSVHLYFQQAFTFRMLTPEAVVGLIA"
     gene            5825..7210
                     /locus_tag="B061_RS0119155"
     CDS             5825..7210
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018487365.1"
                     /locus_tag="B061_RS0119155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_026241685.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 2.9e-37, bitscore:
                     124.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 6.9e-10,
                     bitscore: 34.7, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGAAITDSGRTTQTPTPSTPAFARPAVRFDAAVRAAVGQGLLSES
                     RPVAGFVDTDGVRSSVAELRAAFAHSPGVLHTFAAKASSLIPVLRLLADCGMGCEVASP
                     GELRLAVEAGFAPGTIVLDSPAKTREEIRLALALGVAVNADSIDELRRIDTLRHRDAPS
                     VLGLRVNPQVGGGAIGPMSTATDTSKFGVALRDAGAREAVVRAFEARPWLTRLHAHVGS
                     QGCPLELIAAGVAQTYELAEEINERLGTRQVTSLDIGGGLPVNFTDDTTHPTYAAYVDA
                     LTAAAPGLFSGRYALVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTV
                     FMPDSWPLRVGAFTEDGSPKSGRPLAQDIAGPCCFAGDVVAHGRELPELAEGDFVVLYD
                     TGAYYFSTPWAYNSLPRPAVYGFRSTAPEGEVVFATVREAQTMDAIAGESGLAHADALT
                     GLG"
     gene            complement(7250..7462)
                     /locus_tag="B061_RS38480"
     CDS             complement(7250..7462)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B061_RS38480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_078533168.1"
                     /transl_table=11
                     /translation="MTHPYPDPVPPSPTPPPVPGPPTPVPSPPPVPPGPAPAPPGPAPD
                     PIPQPPAPGPEPVPDPEPQPEPAGS"
     gene            complement(7635..7988)
                     /locus_tag="B061_RS0119160"
     CDS             complement(7635..7988)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513131.1"
                     /locus_tag="B061_RS0119160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_018513131.1"
                     /transl_table=11
                     /translation="MAENAAVEIGDDRERGMLTARADGKVAGTIAYFVMEPAPGALVAV
                     HTVVRPEHEGKGIAGALVRRFYAIAAEEGVPVVPLCPYAAKWAQRHPDEAPAAPEELVR
                     EAERQLKGHPELF"
     gene            complement(8030..8455)
                     /gene="panD"
                     /locus_tag="B061_RS0119165"
     CDS             complement(8030..8455)
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="B061_RS0119165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_018513132.1"
                     /transl_table=11
                     /translation="MLRTLFKSKIHRATVTQADLHYVGSVTIDADLMDAADLLPGELVH
                     IVDIDNGARLETYVIEGVRGSGVIGINGAAAHLVHPGDLVILISYAQVEDAEARAFTPK
                     VVHVDADNRIVALGTDASAPVPGTRTERSPQAVVAGG"
     misc_feature    8488..8501
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.92"
                     /tool="antismash"
     misc_feature    complement(8691..8706)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.42"
                     /tool="antismash"
     gene            complement(9215..9919)
                     /locus_tag="B061_RS0119175"
     CDS             complement(9215..9919)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612835.1"
                     /locus_tag="B061_RS0119175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_043185498.1"
                     /transl_table=11
                     /translation="MDTSSAHQDREEAERLSTAVGVLAAFLSRQPLTQALASLEHRLEG
                     ADGMAVLRIAEDGHVVPELLASAFTARESLGRINDLIHACGILLALPHILGDEERITVR
                     PSLGAGNDPSRPYDLETDQRIAEFKLARWRGADAMRKRQTFKDLVMLAADTSGRRAELF
                     VIGSEPSSFLTTSTSTAAWALDRSPGALRTFTTVFGPPSMSVAQFTATHAAHVQITDLR
                     DLLPAPVVALLQ"
     misc_feature    complement(10034..10049)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     misc_feature    10737..10751
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.57"
                     /tool="antismash"
ORIGIN
        1 ccagcagctc cacgtccgcg ccggctccgg cggcccacgc ctccagcggg tcggagggca
       61 gcaggtcccc gtcggcgacg ggcccgtaga tcgccggatc gtagtggcgc tgcccggagc
      121 cggggtcctg ccggtaccgc tcgacgaccc ggtcggatgc gctcaccagc gtctgcggtg
      181 acgcggcgag cagccccgcg cgggtggcgg gcaccccggc cgccgccgcg acctcggcgg
      241 tggtgcgggc cgcctgtccg accgtggcgc aggcgttgac ggcgctgtgc aggatggccc
      301 ggtggaacag gccacgcgcc cggtcggctg ccatcaggca ggccacggag gtggcacccg
      361 aggactgtcc ggccagagtg acgcgcccgg gtgagccacc gaaggcggcg atggtgtccc
      421 ggacccattc cagggcggcg atctggtcga gcagcccccg gttgtcgggg caggcgggcc
      481 cctcaccgtc ggcgggaacg tggccgaacc cctcgaagcc gaggcggtgg ttgagcgtga
      541 cgacgacgag cccccgggcg gcgagggcgg tgccgtcgaa gtcgggctgg gcggaggagc
      601 cgaagacgta cgcgccgccg tggatccaga cgagcacggg caggtctcgg ggcccgtcgg
      661 ccggtgccca gatgttcagc gtgaggatgt cctcgtcccc gggcgaccag gccggggagc
      721 cgggcagttc ggcggactgg ggcgccacgg gaccgtacgc ggtgcactcc cgtacgccgt
      781 cccagggctg cgccggacgg ggcgcccgga accgttcggc gccgaacggc ggtgcggcgt
      841 aagggagtcc gcgtacggtc acgatccccg ggtcccgctc cgagcgccgc cctcgtacgc
      901 gtcccgcccg cgtggtgaac acatccacgg ctgccgctcc cccgccctgc tccgcccgac
      961 cacccgcccg gcgttcgggc atgcggtggg caggatgtca gccgggggcc cctcgagcca
     1021 acgccttttc gcaggtgggc ggggtgggcc cgggaatcgg tcgcctactg gccgatcagt
     1081 tgcccgcgat gagcacgatg tcggccggtc ccgcggaggc gagggcggag gcgacggccg
     1141 cacggcggtc cgtgaaccgt tcgaacggcg ttccggtcgc cgcgatgccg ggcgcgatct
     1201 ggtcgaggat cgcctcgggg tcctcgtacc ggggattgtc cgaggtgagc acgcacaggt
     1261 ccgagtgggt gccggcgatg gtgcccatct cggcgcgctt ggttacgtac gcgtgcgccg
     1321 ggggcggcgg ctccctccgg cgcggagagg ccggagtcgt tcgtggaagg ggcggcggac
     1381 cggggcgtcc ccttggcccg gatccgcgga cgccgaagac cgctggccgt ggccgtgcag
     1441 tgcggtacgg cccgttccgg gcgccgcgtc ggccggtgcc ggtgcggcgg cggggaccgc
     1501 gaccgggggc agcgcggagg gggcccggtg caggcacttc cccagttcgg cgctacgacc
     1561 caggagccgg gtgtacgtct gctccggcct gccccggtcc gcccggagca ggcggggaca
     1621 tgtcagggcc cggtcttccc tttgcgggcg gagcgggacc ggagtggaat tgcgtacagg
     1681 ggacagtggt tctcccgagg aaaggatgcg cgccatgagc gacaccgact ggggggacga
     1741 cgtctaccag cctcaggagc aggaggctgc cgaccccgat gaacagctcg gctccgagga
     1801 cacgctgctg gacccctccg gcaccggcga tccgctcgac gagggctatt caccgccgga
     1861 ccgcccctgg gtggtcgaag gcctcggcac cacggctgcc gagagcctca cgggtgagtc
     1921 gctcgaaacc cggctgacca gggaggttcc cgagactccc ggtttcaccg gcgacggtgt
     1981 gggagacctg gccggcgggg acggcgagct ctgggacggc aaggtcggcg tcgcccgtgc
     2041 cgggcggctg acccaacagg cggacggcac tgtcccggcc acgctcaccg ccagcgatgt
     2101 gggcatcgac ggcgcggcgg cgtcggccga ggaagccgct atgcacgtga tccccgagga
     2161 gggggacgac gagagccttc tggacggttc gctgcccgac ggcgcgctcg tcgacggtgc
     2221 cgccgaggac ggcgctcctg ttgacagctc ccccgtagag ggctccgtca agggctccgc
     2281 cgaaggcacg tgaacgccgc cgtcccccgt cccgtactcc cctgaccgaa cgcccccgga
     2341 atcgcaggag cacacagatg agcggcactg cccagatcgg cgtcaccgga ctcgcggtga
     2401 tgggccgcaa cctggcccgg aacttcgccc gcaacggctt caccgtcgcg gtgcacaacc
     2461 gcacggcctc ccggaccgac gccctcgtga aggagttcgg cgacgagggc acgttcgtcg
     2521 ccgcgcacac ccccgaggag ttcgtcacgg ctctggaacg cccccgccga ctggtgatca
     2581 tggtgaaggc cggggatccg acggacgcgg tgatccagga gttcgcgccg ctgctggagg
     2641 acggcgacgt catcatcgac gggggcaacg cccacttcga ggacacccgc cgccgggaga
     2701 gggagttgcg ggaacgcggg atccacttcg tcggggcggg cgtctccggc ggggaggagg
     2761 gcgcgctcca cggcccgagc atcatgccgg gcggatcggc cgagtcgtac gcgtcgctgg
     2821 gcccgatgct ggagaagatc gccgcgaagg cgaaggacgg caccccgtgc accgcgcaca
     2881 tcggccccga cggggccggg cacttcgtga agatggtgca caacggcatc gagtacgcgg
     2941 acatgcagct gatcgccgag gcgtacgacc tgctgcgccg gatcgccggg tactccccgg
     3001 cgcagatcgc cgacaccttc cggagctgga acaccggacg gctggactcg tacctcatcg
     3061 agatcaccgc cgaggtcctc gcccatacgg acgcggccac cggcaagccg ttcgtcgacg
     3121 tcgtgcagga ccgcgcggag cagaagggca ccgggcggtg gacggtgcag atcgcgctcg
     3181 acctgggcgt tcccgtctcc gggatcgccg aggcggtctt cgcgcgttcc ctctccggcc
     3241 acacggacct gcgcgaggcg tcgcgggagc tgccggggcc ggtggcgaag ccgctggagg
     3301 gggcggacgc gtcggccttc gcggaccggg tcgagcaggc cctctacgcc tcgaaggtcg
     3361 tctcgtacac gcagggcttc caccagatcc gggccggcag cgaggcgtac gactgggaca
     3421 tcgacctcgg ttcggtggcg gcgatctggc gggcgggatg catcatccgg gcggcgttcc
     3481 tcgaccgcat ccgggccgcc tacgactccc ggccggagct ggtgagcctg ctgtcggacg
     3541 aggagttcgg gcgggagatc ggggccgcgc aggacgactg gcgggcggtg gtggcggagg
     3601 cggcgcgcca gggtgtgccg acgcccggtt tcgccgccgc gctgtcctac tacgacgggt
     3661 tgcgcgcgga gcggctgccg gcggcgctca cgcaggggca gcgggacttc ttcggggcgc
     3721 acacctaccg gcgtacggac cgggaggggg cgttccacac gctgtggggc ggagaccggt
     3781 ccgaggtcga ggcgggctga ggcgtacgca cccggggtgg ggcggccggg tcaccgggtg
     3841 gctcggccgg gtcaccccgg gtgcggctgt ccgcggggca tggcttagtg ggctgcgagc
     3901 cctgttgtac ccggtccccc gacgcctgga gtgccgcgca tgtccgcagc cgtccccgac
     3961 cccgtctccg tccagcccgt cgtggccccg ctgaccaatg cggcgctgtt cctggtcggg
     4021 acggtcgagc cgggcggtga ggacgccgta cgggaggtgc tgccggacct ggcggccgtc
     4081 gcccggtccc tcggcttccg ctacccggac gccggtctcg cctgcgtgac ggggttcggc
     4141 tccgccgtct gggaccgtct gttcggcggg gggcgtcccg cgcacctcca cccgttcccg
     4201 gagctgcggg gccggctcca tcacgccccg gcgaccccgg gcgatgtgct gctgcacatc
     4261 cgggcggagc ggatggacgt ctgcttcgag tgggcggcgc agctgctcac caagctcggc
     4321 ggggcgctgc ggatcgtgga cgagacgcac ggcttccgct atctcgacca ccgggatctg
     4381 ctgggcttcg tggacggtac ggagaatccg gtcggcgacg acgcccgctc ggcggcgctg
     4441 gtcggtgcgg acgacgatcc ggggttcgag ggcggcagtt atgtcgtcgt acagaagtat
     4501 ctgcacgacc tgacgtcctg gaacgcgctc tccgtggagg agcaggaacg cgtcatcgga
     4561 cgcacgaaac tcgacgacgt ggagttcccc gacgacgaga agcccgccga ctcccatctc
     4621 gcgctcaaca cgatcaccga cccggacggc accgaacgcg acatcctgcg ggcgaacatg
     4681 cccttcggga acttcggtca gggcgagttc ggcacgtact tcatcgggta cgcggccgac
     4741 cccgacgtca ccgaacgcat gctccgcaac atgttcctcg gcgatccgcc cggcacgcac
     4801 gaccgcatcc tcgacttctc cacggcggtc accggaggcc tcttcttcgc tccgcgcgcc
     4861 gagttcctcg acgacccgcc gcccccgccc tctccggtcc ccgccgtgga ccggccggaa
     4921 ccagtgcccg tcgcggctgc accggatggg ccggagcacg gttcgctgcg catcggcagc
     4981 ctgcaagaaa gcgcccagtg atgaacaatc tgcatcgcga gctcgcaccg gtcacccccg
     5041 ccgcctggga cgagatcgag gaggaggcgc ggcgtacgtt ccgccgccat gtcgcgggcc
     5101 gccgcgtcgt cgacgtctcc gatccggacg gcccgacgct ggccgcagtg ggcgacggcc
     5161 atctgcgcga catcgacccg ccgacgccgg atgtggtggc ccgggcccgt acatcgatgc
     5221 cggtcatcga gtggcgggtg ccgttcacgg tgagcaggca ggcggtggac gacgtggagc
     5281 gcggctccgc agacagcgac tggcagcccg tcaaggacgc ggcgcgcacc tgtgcgttcg
     5341 cggaggacat ggcggtcatc gacgggtacg cggcggccgg gatcaccggt ctgcgggacg
     5401 gctcctcgca cgacccgctg ccgctgcccg ccgacgcccg ggactatccg gtggcggtca
     5461 gccaggcggt gacgcggctg cggctggccg gtgtcgaggg gccgtaccgg ctgctgctcg
     5521 gcgcggacgc gttcacggag gccgccgaga tgtcggacca cggctttccg gtcaagaccc
     5581 atctggcccg tatcgtggac gacgagatcc tgtgggcgcc cgccgtgaag ggcggggtgc
     5641 tgctgtcgac ccggggtggc gacttcgaac tctgcctggg ccaggacctg tcgatcgggt
     5701 acgcggacca tgacgccacc agcgtccacc tctacttcca gcaggcgttc acgttccgga
     5761 tgctgacgcc ggaggcggtg gtggggctca tcgcctgagg tccagccgct ttcgccgaag
     5821 acctgtgggg gcagccatca ccgactccgg ccgtacgaca cagacgccta cgccatctac
     5881 tccagctttc gcgcgccccg ccgtccgttt cgacgcggcc gtccgggcgg ccgtcgggca
     5941 ggggctgctg agcgagtccc gcccggtggc cgggttcgtc gacacggacg gggtgcgctc
     6001 gtccgtcgcc gagctgcggg cggcgttcgc gcacagcccc ggggtgctgc acaccttcgc
     6061 ggccaaggcc tcctccctca tcccggtcct gcggctgctc gccgactgcg ggatggggtg
     6121 cgaggtggcg agcccgggtg agctgcggct cgccgtcgag gccggtttcg ctccggggac
     6181 gatcgtcctg gactcccccg ccaagacccg cgaggagatc cggctggcgc tcgccctcgg
     6241 ggtggccgtc aacgccgaca gcatcgacga actccgccgt atcgacacac tgcggcaccg
     6301 cgacgccccc tccgtcctcg gactgcgggt caacccgcag gtgggcggcg gggcgatcgg
     6361 cccgatgtcc acggcgacgg acacctcgaa gttcggggtg gcgctccgtg atgcgggcgc
     6421 gcgcgaggcg gtcgtacggg ccttcgaggc gcgcccgtgg ctgacccgcc tgcacgccca
     6481 tgtcggctcc cagggctgcc cgttggagct gatcgcggcg ggcgtggcgc agacgtacga
     6541 gctggccgag gagatcaacg aacgcctcgg cacccggcag gtgaccagcc tggacatcgg
     6601 cggcgggctg ccggtcaact tcacggacga caccacgcac ccgacgtacg cggcgtacgt
     6661 ggacgcgctg acggcggcgg ccccgggcct gttctccggc cggtacgcgc tggtcaccga
     6721 gttcggccgg tcgctgctcg cgaagaacgg cttcatcggg gcgcgggtgg agtacacgaa
     6781 ggacgccggc ggccgccgga tcgcgctcac ccacgcgggg gcgcagaccg ccacccgtac
     6841 cgtcttcatg cccgactcct ggccgctgcg ggtcggggcg ttcacggagg acggctcgcc
     6901 gaaaagcgga cggccgctgg cacaggacat cgcggggccg tgctgcttcg cgggcgatgt
     6961 ggtggcgcac ggccgggagc tgccggagct ggccgagggc gatttcgtcg tcctgtacga
     7021 cacgggggcg tactacttct ccaccccgtg ggcgtacaac agcctgccgc gcccggcggt
     7081 gtacggcttc cgctcgacgg cgccggaggg cgaggtggtg ttcgcgacgg tacgggaggc
     7141 ccagacgatg gacgcgatcg cgggggagag cggcctggca cacgcggacg cgctgacggg
     7201 gctgggctga gtgggggctc cgtacgaacg gagcccccag gccactcctc tacgagccgg
     7261 cgggctcggg ctgcggctcc gggtccggta cgggctcggg gccgggcgcc ggaggctgcg
     7321 gaatcgggtc cggggccggg ccgggcggcg ccggggcggg ccccggcggc acgggcggcg
     7381 ggctcggcac gggggtgggc ggacccggca cgggcggagg cgtcggcgac ggcggtacgg
     7441 ggtcggggta cgggtgggtc atttcgatcc tccacaacga cacgaaacgg acagatggag
     7501 ctttcctcca ccgtctcccg gtgcggctgc ccgcgcctgc cgagtccaca cgtacgacgc
     7561 agggcggagt gcgggttcgc gggacaagcg cccgccaccc ctccctctcc ttctcctgct
     7621 cctcctgctc cccctcagaa cagctcggga tggcccttga gctgccgctc cgcctcccgt
     7681 acgagctcct ccggagccgc gggtgcctcg tcggggtggc gctgcgccca cttcgccgcg
     7741 tacgggcaca ggggcacgac ggggacgccc tcctccgcgg cgatggcgta gaacctccgg
     7801 acgagggctc cggcgatgcc cttgccctcg tgctcggggc ggaccaccgt atggacggcc
     7861 acgagcgcgc ccggggcggg ctccatgacg aagtaggcga tcgttccggc gacctttccg
     7921 tccgcccggg ccgtgagcat gccgcgctcc cggtcgtcgc cgatctctac agctgcgttc
     7981 tccgccatgg gcttccgctc tcctcgttcc gtgggcacgg acggccggtt cagccgcccg
     8041 cgacgaccgc ctgcgggctc cgctccgtac gggtcccggg caccggggcc gacgcgtcgg
     8101 tgccgagcgc cacgatccgg ttgtccgcgt cgacatggac gaccttcggg gtgaacgcgc
     8161 gggcctcggc gtcctcgacc tgtgcgtagc tgatcaggat gaccaggtca ccggggtgca
     8221 cgaggtgtgc ggcggcgccg ttgatcccga tgacaccgga gccgcgcacg ccctcgatga
     8281 cgtacgtctc caggcgggcg ccgttgtcga tgtcaacgat atggaccagc tcgccgggca
     8341 gcaggtcggc ggcgtccatc aggtcggcat cgatcgtcac ggagccgacg tagtgcaggt
     8401 cggcctgggt gacggtggcc cggtggatct tggacttgaa cagagtgcgc agcacggtgg
     8461 actcctctga gacggctccc tgcctgcttt atgcaggtca agggcgatct cgactgtaca
     8521 ccggcacacg ccgaccgagc caaggtccat gtcacgcgcc cctgcacact ggcgtgcgcc
     8581 gctcctccct tccgtacggt ccgaccgcca ctacaagtcg ctgcagtccg gcgagctcgg
     8641 catcggcttc gcactcaccc tcgccgaaca cctgctgcgc agccgcttcc ccgaccacac
     8701 ggtgacgatg gtccccgctg acacggcgtt acgcgcgggc tggcctctca ccagcagaga
     8761 caagggcgag aaggtgaagt accgctacct tcctcagtac ttcgcagagg tctggcggcc
     8821 gggcgagccg tccctcacca tccccctcgc ctgcaagggc aaccacggcg acaccgccac
     8881 ctccgccgaa cagctggctt ccgcctccgc ccacgccgaa gcggtccaca tcggcccgtg
     8941 gaacgagacc cccagcctgt tgttcagcac gcagctcccg acgggcggcg gcacgatgac
     9001 cgtccacgcc cttcaggcac ccggcagcgg cggccggctt caccttcgac gaggactggg
     9061 gcggccccgt ctccgtgcaa gccgacggtt cggttctcgc cctgcgtctg ctgccgggac
     9121 aggccgaggc gcctcgtcct gtccgtggac aatccgggtg cgccgatcga tcacgtcggg
     9181 aaacgctgtg cggttgaact ccgcagccta cgtatcactg caggagggct acgacgggcg
     9241 cgggaagaag atcccgcaga tcggtgatct gaacgtgggc ggcatgggtc gcggtgaact
     9301 gggcgacgga catgctcggt gggccgaaga cggtggtgaa ggtccgcagc gcccctgggc
     9361 tgcggtccag ggcccaggcc gctgttgaag tgctggtcgt caggaagctg ctgggctcgg
     9421 aaccgatcac gaacagctct gcccggcggc cggaagtgtc ggcggcgagc atcacgaggt
     9481 ccttgaaggt ctggcgcttg cgcatggcgt ccgcgccgcg ccaacgagcg agcttgaact
     9541 ccgcgatgcg ctggtctgtc tccaggtcgt acggacggct cgggtcgttg ccggcgccga
     9601 gggaggggcg gacggtgatg cgttcctcat ccccgaggat gtggggcagg gcgaggagga
     9661 tgccgcaggc gtggatcaag tcgttgatcc ggcccaggct ttcgcgggcc gtgaacgctg
     9721 aggcaagcag ctccggtacg acgtggccgt cctccgctat ccgcaggaca gccatcccgt
     9781 cggcgccctc caatcggtgc tccagcgagg ccagggcctg ggtgagcggc tggcggctca
     9841 ggaaggcggc gaggaccccc acagcggtgc tcagccgctc tgcttcttca cggtcctggt
     9901 gggctgagga ggtgtccatg gggacagtct ggctcttctt cggtgcggtg aagccggagg
     9961 agacgtgccc ggccggcgac aaacaggctc ctgaagcggc gggcgggaag atgccgggcc
    10021 tggttgagga gtccgtgaag gcgttcacgc cggagcatga gggggattgc tcggatacgt
    10081 cgctggacca ggcggactgg gtggagcact gctcccaggg gtgacggagt tgggactgtc
    10141 actgaccgca gcgcctctgc gctgtccatc tggtgtccgg tgagaagcga tgggagggcc
    10201 atgtttcagg tgtattcggg tgtagagcgt cactctcgcg ccgttgacct catgggtcgt
    10261 ctgtgcgcgg aagtgacgtc gcagagtgtc ccttttcgct tcctctcgcg gatcaccggg
    10321 cgtgtgcatg cctactgccc ccaccacgac gatccggtcg aactgctgca tacgcgcggc
    10381 gatctcaggg gcagggcgct caacacccgc gagggtgttc gatgccacgg ggtctcgcgc
    10441 cagggcgata tcgaccagga ggcgggtgtc gtcggggtcg gcggcggtca gcgtccgttg
    10501 ccggccggag aggtagagca gcccgtcgcc ggggcggcct tcggtgcgga tggcggcgcc
    10561 gatggcggtg gcgtcgttgc tccggctctg gggcgtcctc agcgagctgc tcagcggtac
    10621 gagcgcgacc agagccgcgg gcaccgcgac gtacacggtc caggagtatc gcggcagctt
    10681 gtggacgtac tccacgaagg cgcccagcag gagggcgagc ccgatgtcgc tgaagaggac
    10741 gtaacggtcc acgaagatcg gcttgatcat ggagaccatc agcagcagag cacctggg
//
