LOCUS       NZ_KB891296            22647 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. ScaeMP-e10 B061DRAFT_scaffold_0.1, whole genome
            shotgun sequence.
ACCESSION   NZ_KB891296
VERSION     NZ_KB891296.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. ScaeMP-e10
  ORGANISM  Streptomyces sp. ScaeMP-e10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1873521)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Improved High-Quality Draft of Streptomyces sp. ScaeMP-e10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1873521)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 1873521)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. ScaeMP-e10
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            Isolation Site                    :: Gulf of Mexico
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,185
            CDSs (total)                      :: 7,109
            Genes (coding)                    :: 6,996
            CDSs (with protein)               :: 6,996
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 1 (5S, 16S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 23 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1684863
            Orig. end                         :: 1707510
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB891296.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405355
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22647
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="5"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22647
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22647
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12647](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12647)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    43..63
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.01"
                     /tool="antismash"
     misc_feature    55..71
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.22"
                     /tool="antismash"
     gene            641..1339
                     /locus_tag="B061_RS0107885"
     CDS             641..1339
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382784.1"
                     /locus_tag="B061_RS0107885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_018511001.1"
                     /transl_table=11
                     /translation="MTEPLRVLLADDQTLVRTGFRLILRADGIDVVAEATNGAEAVDAV
                     RRTRPDVVLMDVRMPEMDGLEATRRILTGTADAPRVIILTTFDLDRYVYAALSAGASGF
                     LLKDVTPEQLTAAIRTVRTGDALLAPAITRRLVQRFTRRGSETAALHRDLTSLTPRELE
                     VFGLLARGLSNAELATRLHLAEATVKTHVARILAKLGLRDRVQAVIVAYETGLVSVGAD
                     EAARQSAEET"
     gene            complement(1415..2380)
                     /locus_tag="B061_RS0107890"
     CDS             complement(1415..2380)
                     /NRPS_PKS="Domain: PKS_ER (28-318). E-value: 6.7e-37.
                     Score: 119.2. Matches aSDomain:
                     nrpspksdomains_B061_RS0107890_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047546.1"
                     /locus_tag="B061_RS0107890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_018511002.1"
                     /sec_met_domain="ADH_N (E-value: 5.1e-09, bitscore: 32.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 6.1e-14, bitscore:
                     48.9, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDMRAALYDSYGPPEVLYEGRVPVPVRGPGEVLVRVHAASVNGG
                     ELYGRAGKVRLVTGRRFPQRAGIDFAGEVAEVGPSVTGLSVGDRVWGILPRGRGGFGSA
                     AEYVSVRPRQIAYAPENVTLTEAASLPAGGTTSLTALRDKARLRAGERLLVRGASGGVG
                     SVAVQVGKVLGGHVTGLAGAKSLDFVRELGADVVLDHRSVELSELGPYDVIMDTVGSEH
                     RTLRRLLAPGGRLVSIAFDIHHPVRGLGYILGSAVHGSRRVRFFSGNPKHDLFAELTAY
                     VERGDLRPVVDTVHSLADIAAAHRALEAGGVRGKHVIRVV"
     aSDomain        complement(1427..2296)
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B061_RS0107890_PKS_ER.1"
                     /evalue="6.70E-37"
                     /label="B061_RS0107890_PKS_ER.1"
                     /locus_tag="B061_RS0107890"
                     /protein_end="318"
                     /protein_start="28"
                     /score="119.2"
                     /tool="antismash"
                     /translation="GPGEVLVRVHAASVNGGELYGRAGKVRLVTGRRFPQRAGIDFAGE
                     VAEVGPSVTGLSVGDRVWGILPRGRGGFGSAAEYVSVRPRQIAYAPENVTLTEAASLPA
                     GGTTSLTALRDKARLRAGERLLVRGASGGVGSVAVQVGKVLGGHVTGLAGAKSLDFVRE
                     LGADVVLDHRSVELSELGPYDVIMDTVGSEHRTLRRLLAPGGRLVSIAFDIHHPVRGLG
                     YILGSAVHGSRRVRFFSGNPKHDLFAELTAYVERGDLRPVVDTVHSLADIAAAHRALEA
                     GGVRGKHVI"
     CDS_motif       complement(1874..1933)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B061_RS0107890_0001"
                     /evalue="5.70E-05"
                     /label="PKSI-ER_m5"
                     /locus_tag="B061_RS0107890"
                     /protein_end="169"
                     /protein_start="149"
                     /score="15.8"
                     /tool="antismash"
                     /translation="GERLLVRGASGGVGSVAVQV"
     gene            2481..3185
                     /locus_tag="B061_RS0107895"
     CDS             2481..3185
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457005.1"
                     /locus_tag="B061_RS0107895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_018511003.1"
                     /transl_table=11
                     /translation="MPAQPSHNPPPTGSEALRAEALRTSALRTDALRADARENRDRILR
                     AAREAYALHGIDVPISAIARRAGVGVATLYRRFPTRASLITEAFAEQLAECAGALDEAL
                     EDPDPWRAFCSVLTKVCAMQAADRGFTHAFLARFPDESSYAGERDRAEEGLALLVRRAK
                     EAGALRPDFDVSDVVLALLANSGVVNGVQPEAGATSRRLVGYLLESFRTEGAVRPLPPP
                     VRLELDQLYGAG"
     gene            complement(3651..4943)
                     /locus_tag="B061_RS0107905"
     CDS             complement(3651..4943)
                     /GO_function="GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047360.1"
                     /locus_tag="B061_RS0107905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mannosyltransferase family protein"
                     /protein_id="WP_199784457.1"
                     /transl_table=11
                     /translation="MSTLPPGLRPAPPRPRGPESRPVTHEFRPAEKPVPHHPRPAPRAL
                     RAAGFDRAAAWLSPADRRVLWLYLLTRISVWITAHGARWLFPRGSDVREAGPVLAPFQQ
                     WDANHYLHIARDGYFPAGTGPGMDGWDNREAFFPGFPLLLRAVHVVIPSWTAAGLLISL
                     VAGAAAVLALSRLASSYVSEDTDGRRTALFFLLSPCAVFLAVGYTEALFLAFALPAWLA
                     AQRHRWAPACALTALATTVRVSGLFLAAAIAVLFALSAWSGKGWRGAPWLALPALPPLA
                     YSWYLHAHTGDWMAWKHAQERGWYRDFHTPWEAWSNTWNAAFEGAHTTGYAAMFQAELA
                     AMLVGLALVALLVRHRRWPEAVYVALSLWALGTSYWYVSIPRATLLWWPLWIGLAALSL
                     RRPWFRTVYLCVTVPVSALVALAFLTGRWAG"
     gene            complement(5043..5516)
                     /locus_tag="B061_RS39460"
     CDS             complement(5043..5516)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010055997.1"
                     /locus_tag="B061_RS39460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_158689932.1"
                     /transl_table=11
                     /translation="MSEPRDRSGPEASRGAQDPLRSLFRQAGDFGQGHVVPEPATYITE
                     RGMRAQRRRMAAVAVGVCLVIGGGGAAAVGLLPVTPKPAPPATSPSPSYPSPAPPPPAG
                     TPTEPPTPSNTVPGSEGAVTGTTAPGGLPTTEAPTSASSTSTSTSTLGATTMP"
     gene            complement(5509..6009)
                     /locus_tag="B061_RS0107915"
     CDS             complement(5509..6009)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489040.1"
                     /locus_tag="B061_RS0107915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_018511007.1"
                     /transl_table=11
                     /translation="MTVEEFEEFYAQAVTRLTGQLYVMTGDLQEAQDVVQEAFVKAWVR
                     RGRLERDGQPEAWIRTVAWRLAVSRWRFRRRSADAWGRGSGAAEHVAPPDPDHVVLVDA
                     LRELPAQQRRTLTLHYLCDLTVEQIAGETGLSASTIKTHLVRGRAALAHRLQDPRMEEA
                     PDV"
     misc_feature    5981..5994
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.98"
                     /tool="antismash"
     misc_feature    complement(6105..6115)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            complement(6156..6419)
                     /locus_tag="B061_RS0107920"
     CDS             complement(6156..6419)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B061_RS0107920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_018511008.1"
                     /transl_table=11
                     /translation="MTFTVHSPNGRFVTLGATEVATRARAASSAAREHTVTVGGLPGVI
                     SWSEDGTPPSLLAFTVADGRITGITALIDPARLALMDLPHPV"
     misc_feature    6655..6671
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.78"
                     /tool="antismash"
     gene            6867..7685
                     /locus_tag="B061_RS0107925"
     CDS             6867..7685
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958801.1"
                     /locus_tag="B061_RS0107925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018511009.1"
                     /transl_table=11
                     /translation="MAKPGYGKRSAPGERPRTDRDFAHLLPRDAAVAAYIDRLPDGSDI
                     SVKTLAKHLPYGQCALSTTLNRLQDAGHLRRGSECVITDEVMFWVTRTFFSRTAREHEW
                     WNAYIRGDVPPDRPQRPTRSRAFILLAALGRTAPALSLSAADCVRLEPQVSEWFARGAT
                     EAELLHALTAGLPSPVHCAARLVERRLRDKLPPERNVPGPRTVLRTLECGQCGVPGSPE
                     ALLGGLCRRCRGPVPELPIGPPGAVQPPGVRERMDAVRAGLVPRSERQHQ"
     gene            7682..8215
                     /locus_tag="B061_RS0107930"
     CDS             7682..8215
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610350.1"
                     /locus_tag="B061_RS0107930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Uma2 family endonuclease"
                     /protein_id="WP_018511010.1"
                     /transl_table=11
                     /translation="MIPVQDFEALARVCPETVTLELINGKLEVKPVPDGDHDEIIMWVA
                     MQCVRQRPELRLYRTRGLRIGGDRNDRARPDGSLARAGRFAGDGAWSSPDRILMTVDVT
                     SYDDSPVQPCGYAAAGIPLRLLIDRRSGTFAVRSEPHSARYRHQRLYDIGEAVALPRPA
                     SITLDTEELKNYTG"
     gene            complement(8203..9996)
                     /locus_tag="B061_RS0107935"
     CDS             complement(8203..9996)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610349.1"
                     /locus_tag="B061_RS0107935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_018511011.1"
                     /transl_table=11
                     /translation="MGAPEDAKEPGAEAPARSGPSPLRLLLTRVRPHRGVLIRAGLLSL
                     TGSAAGLAMPLMAKYAVDAFAAGSSPVAPLVALTALVLAGACLSAYGRYLMARTGEGVV
                     LRARHQLVGRIMRLKVPAVDRLTPGDLQSRVTSDTTLLRTVLSSGLVESFNSVLMLLGT
                     VAFMAYMDVTLLGVTLVVIVGIGAVTALLMPRIQRAQLRAQESVGAMGAALDRVLQAFR
                     TVKASGAEERETAAVAEAARHAHDRGVSVAKWSSVSDVTMMMSIQLAFLAVLGVGGARV
                     ASGSLDISSLIAFLLYLFYLMGPIGGLVEGWTGLQSGLAAVRRIDEVESLPGEPAEEFG
                     GLKEEGGGPLRVTFQDVTFGYGDERAPAHKGVTFEVPAGGMVALVGPSGAGKSTVFSLL
                     ERFYDHDGGTITVDGRDIRDWPLTELRASLAYVEQDAPVLAGTLRENLLFAAPDAPEAR
                     LAAAVARTRLDALVARLPDGLDTAVGHRGVTLSGGERQRIAIARALLRRPRLLLLDEVT
                     SQLDAVNEQALRDVILELAEQTTVLVIAHRLSTVRHADRIVVLEEGRVRTAGTHEELIA
                     SDDLYRELATTQLAAAADQPV"
     gene            complement(10001..10471)
                     /locus_tag="B061_RS0107940"
     CDS             complement(10001..10471)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489034.1"
                     /locus_tag="B061_RS0107940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_018511012.1"
                     /sec_met_domain="PF13471 (E-value: 1.7e-25, bitscore: 86.1,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEMTMPRRGAGPGGLRLRTAIAAAFVLARFKPGRLRRVLTRLS
                     RGANPATYDETLEMYEAVTATSRRCAGRYGCLPRSVAIALACRMSGSWPDWCAGVRTAP
                     PFSPHAWVEAEGRTVGEQAEAADLRPLMVVTVREGVPGERGGGNDDGGGQAP"
     gene            complement(10468..10719)
                     /locus_tag="B061_RS0107945"
     CDS             complement(10468..10719)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126488.1"
                     /locus_tag="B061_RS0107945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_018511013.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2e-26,
                     bitscore: 88.6, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.7e-13, bitscore: 46.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSVIVVETLAAGEA
                     PEAAVERIVAEFDVERAEAESDVAQLTRQLVEAKILRP"
     aSDomain        complement(10474..10719)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_B061_RS0107945_Stand_Alone_Lasso_RRE.
                     1"
                     /evalue="3.20E-28"
                     /identifier="RREFam002.1"
                     /label="B061_RS0107945"
                     /locus_tag="B061_RS0107945"
                     /protein_end="82"
                     /protein_start="0"
                     /score="88.6"
                     /tool="antismash"
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSVIVVETLAAGEA
                     PEAAVERIVAEFDVERAEAESDVAQLTRQLVEAKILR"
     gene            complement(10716..12647)
                     /locus_tag="B061_RS0107950"
     CDS             complement(10716..12647)
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010071617.1"
                     /locus_tag="B061_RS0107950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_018511014.1"
                     /sec_met_domain="Asn_synthase (E-value: 1e-35, bitscore:
                     120.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGLLRDYFVVLPDSVAGHAVAGQLPVPDTTRTAGGAGGTVTVAH
                     PSGRPWVVARPLVRKVSHLRRGDDALVLIGPDQVPEAVLAGLLEGARDRAGLERRLARL
                     PGLHHVIAYLSGETWIRGTASGLRRIYHARPAEAGTIASDRPAVLAHLTGAPLDDGALA
                     LRMLDFLPHPLSRRVLWRGVHETGAGYGLALPAAAPGTAAAPGPREYRWWEPPPPELPL
                     AEGARRLGDAVAASVRAHIGGLDRISCELSGGLDSTTLTFAARATAPAELSLLTVAARD
                     RYSEDETWARRAVEAARGGHTDTPALAHHIIPADQAPYFYADLAATSAELNDEPLPVAP
                     GRARAHLLLGRAHATGSRYHLTGYGGDELFLGLPHAYQDLFHGNPLTAWNHLSGLRHQL
                     GWPLLPTLKALLNRSTFPRWLAGAVTTEPQPVTRTPLLSWGVRQSLRPWFTDHANALIT
                     EEFRAAAEQAEPIDRWRGRHVDIDAVRMGARHFQAMEDVGMTIGLPVAAPFYDDRVLEA
                     TLAVRLEERISPWRYKPLLVEAMRGVVPDALLARTTKDHMSSDEHQGLREHASELADLW
                     TGSRLAEHGLVDARHLLRLAAEPFSPVLVEHSISSTVAGETWLRTAENAWPPPDRTPTT
                     ISPSEAAR"
     gene            complement(12698..12820)
                     /locus_tag="B061_RS39115"
     CDS             complement(12698..12820)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489031.1"
                     /locus_tag="B061_RS39115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_018511015.1"
                     /transl_table=11
                     /translation="MKQQKKAYVKPSLFKQGDFSKKTAGYFYGSYKEYWVRRII"
     CDS_motif       complement(12749..12820)
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKQQKKAYVKPSLFKQGDFSKKTA"
                     /locus_tag="B061_RS39115_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(12698..12748)
                     /prepeptide="core"
                     /locus_tag="B061_RS39115_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="0.20"
                     /molecular_weight="2082.4"
                     /monoisotopic_mass="2081.0"
                     /core_sequence="GYFYGSYKEYWVRRII"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKQQKKAYVKPSLFKQGDFSKKTA"
                     /leader_location="[12748:12820](-)"
     misc_feature    complement(13085..13100)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.0"
                     /tool="antismash"
     gene            complement(13394..13960)
                     /locus_tag="B061_RS0107960"
     CDS             complement(13394..13960)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126485.1"
                     /locus_tag="B061_RS0107960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_234017743.1"
                     /transl_table=11
                     /translation="MDTARAVVRIRGGAGMSVSAAGRRGGGRLKGASLVVGGAVLFAAG
                     LFIVVTGTGLSGLLGMRVETFGRIDCRDTRTEKGQTVWHCFGETPAQQRANEAERKRVA
                     DEALRAHVDGMPESARIERTRILFADHDGRSNPETITATQVTDGGRWFAHSSTVVGYGM
                     IPLLLGAGTAAWGGYRMREAGRRGR"
     gene            complement(14079..15701)
                     /gene="groL"
                     /locus_tag="B061_RS0107965"
     CDS             complement(14079..15701)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /gene="groL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /locus_tag="B061_RS0107965"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="chaperonin GroEL"
                     /protein_id="WP_018511017.1"
                     /transl_table=11
                     /translation="MAKIIAFDEEARRGLERGMNQLADAVKVTLGPKGRNVVLEKKWGA
                     PTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLR
                     NVAAGANPMALKRGIEKAVEAVSAALLEQAKDVETKEQIASTASISAADTEIGAKIAEA
                     MDKVGKEGVITVEESQTFGLELELTEGMRFDKGYISAYFATDMERMEASLDDPYILIVN
                     SKIASVKDLIPLLEKVMQSGKPLLIIAEDVEGEALSTLVVNKIKGTFKSVAVKAPGFGD
                     RRKAMLADIAILTGGTVISEEVGLKLESAGLDLLGRARKVVITKDETTIVDGAGDSEQV
                     QGRVKQIRAEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVR
                     NAKAAVEEGIVAGGGVALLQASAVFDKLDLTGDEATGANAVKLALEAPLKQIAVNGGLE
                     GGVVVEKVRNLPIGHGLNAASGEYVDMIAEGIIDPAKVTRSALQNAASIAALFLTTEAV
                     IADKPEKAGAAAPGGMPGGDMDF"
     misc_feature    15819..15834
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.09"
                     /tool="antismash"
     misc_feature    15853..15868
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    complement(15856..15871)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            complement(16045..16251)
                     /locus_tag="B061_RS0107970"
     CDS             complement(16045..16251)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967346.1"
                     /locus_tag="B061_RS0107970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cold-shock protein"
                     /protein_id="WP_003967346.1"
                     /transl_table=11
                     /translation="MAQGTVKWFNAEKGYGFIAVDGGADVFVHYSAIQMDGYRTLEEGQ
                     RVEFEISQGQKGPQADMVKLAVG"
     misc_feature    complement(16454..16468)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.29"
                     /tool="antismash"
     gene            complement(16711..16989)
                     /locus_tag="B061_RS0107975"
     CDS             complement(16711..16989)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265553.1"
                     /locus_tag="B061_RS0107975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MoaD/ThiS family protein"
                     /protein_id="WP_018511018.1"
                     /transl_table=11
                     /translation="MSVKVRIPTILRTYTGGQAEVTAEGAKLSEVIDSLEKDHPGIAAR
                     VLDDQGKLRRFVNVYVNDDDVRFEGGLDAATPDGAGISIIPAVAGGC"
     gene            complement(17019..18314)
                     /gene="thrC"
                     /locus_tag="B061_RS0107980"
     CDS             complement(17019..18314)
                     /EC_number="4.2.3.1"
                     /GO_function="GO:0004795 - threonine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thrC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056010.1"
                     /locus_tag="B061_RS0107980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="threonine synthase"
                     /protein_id="WP_018511019.1"
                     /sec_met_domain="PALP (E-value: 2.4e-61, bitscore: 204.4,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVQTIAGNATTASVDLGPAVALSCRECGERFELGPLFACASCFG
                     PLEVAYDLPTGSPEELRKRIEAGPNNIWRYAPLLPVPADVAEKPNINPGFTKLVKADNL
                     ARELGVTGGLYVKDDSGNPTHSFKDRVVAIAVEAARAFGFTTLSCSSTGNLAGAVGAAA
                     ARAGLRSCVFIPHDLEQGKVVMAAVYGGELVGIEGNYDDVNRFCSELIGDPLGEGWGFV
                     NVNLRPYYGEGSKTLAYEICEQLGWKLPDQIVIPIASGSQLTKIDKGFQELIKLGLVED
                     KPYKIFGAQAEGCSPVSAAFKAGHDVVRPQKPNTIAKSLAIGNPADGPYVLDIARRTGG
                     AVEDVNDEQVVDAIKLLARTEGIFGETAGGVTLGVTRKLIEAGVIDPALTTVVLNTGDG
                     LKTLDAVSATSQATATIKPSLDAFRAAGLASA"
     regulatory      complement(18333..18460)
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    complement(18568..18588)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.36"
                     /tool="antismash"
     gene            18625..19575
                     /locus_tag="B061_RS0107985"
     CDS             18625..19575
                     /EC_number="2.4.1.266"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511020.1"
                     /locus_tag="B061_RS0107985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="WP_018511020.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.3e-10,
                     bitscore: 37.3, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLEEVERWLTRRSWSSADRPLNRLTDARAADPHRTSVSVVLPALN
                     EEATVGAIVATIRRELMEKVRLVDELVVIDSGSTDATAAVARAAGARVVHRDAILPRIP
                     ALPGKGEVLWRSLLVTSGEIVCFIDADLKDFSADFVSGTVGPLLTDPAVQFVKAMYDRP
                     FGDTAGQGGRVTELVARPLLNLHWPQLAGFVQPLGGEYAVRRSLLERLPFPVGYGVELG
                     LLVDALHTVGLDALGQVDVGVRRHRHQDGQALGRMAAAIYRTAQLRLSRGHLVRPALTQ
                     FERGAEGFVPRTHVVDTEERPPMREIEEYVERWAA"
     gene            19688..21079
                     /locus_tag="B061_RS0107990"
     CDS             19688..21079
                     /EC_number="2.4.1.15"
                     /GO_function="GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101576.1"
                     /locus_tag="B061_RS0107990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="WP_018511021.1"
                     /transl_table=11
                     /translation="MVTEHAPSSTAQVLVASNRGPITYTLGEDGTLDAKRGGGGLVSGL
                     SAVDDKLWVCAALGDGDREAVRRGVGEPGVRMLDIDAEVHADAYNGIANSVLWFVHHLL
                     YQTPVEPVFDAEFRRQWASYEAYNRAFAQALAEEAGEGAAVLVQDYHLALVPGMLRELR
                     PDLRIGHFSHTPWAPVDYFRLLPDDIGEQLLRGILGADRAAFLTRRWADAFIGCCTEIL
                     GGTGRTRIGVHGLGADADFLRRRSQEADVDERMEALREQVGEGRKTIVRVDRTELSKNI
                     VRGLHAYRALLETHPEWRERVVHVAFAYPSRQDLAVYRDYTAAVQTLATEINGAYGTES
                     WTPVILHVNDDFARSLAAYRLADVALVNPIRDGMNLVAKEVPVVSDHGCALVLSREAGA
                     YEELGEDAIVVNPYDVVGTAEALHEALSMGGDERSGRTKRLAEAATALPPQQWFLDQLE
                     ALRQE"
     gene            complement(21104..21961)
                     /gene="otsB"
                     /locus_tag="B061_RS0107995"
     CDS             complement(21104..21961)
                     /EC_number="3.1.3.12"
                     /GO_function="GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="otsB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958814.1"
                     /locus_tag="B061_RS0107995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trehalose-phosphatase"
                     /protein_id="WP_018511022.1"
                     /transl_table=11
                     /translation="MGSHSVQPPIPTPTTPAGREGLEAILARPDRAVIALDFDGTLADI
                     VPDPERARAHPGAVEALAALAPKVASVAVITGRPAGVAVRYGGFAGVPGLDHLVVLGHY
                     GAERWDAATGTVNAPAPHPGVAAVRAELPGVLHEFDSWHGTWTEEKGQAVAVHTRRADD
                     PQAAFETLRGPLGELAARHGLILEPGRQVLELRPPGMDKGVALATYVAEVDAESVLYAG
                     DDLGDLAAFAAVEKLRGAGPDGIPGLLVCSGSAEVPELAERADLLVPGPAAVVDFLAAL
                     ADRL"
     gene            complement(22130..22390)
                     /locus_tag="B061_RS0108000"
     CDS             complement(22130..22390)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610337.1"
                     /locus_tag="B061_RS0108000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3263 domain-containing protein"
                     /protein_id="WP_018511023.1"
                     /transl_table=11
                     /translation="MTAPDAEPEPVAPGLSDRDRALLALERRSWAGPGAKERAIREELG
                     ISPVRYYQLLNALIEDERALREDPVTVNRLRRLKAARRERR"
ORIGIN
        1 agcgggccag gatcgcccgc gaactgcacg acgtcgtcac gcacaacgtc agcgtgatga
       61 tcatccaggc aggggccgcc cggaagatca tgaagatctc ccccgaggag gccggcgagg
      121 cactgctcgc cgtcgaggcc ggcgggcggt cggccatggc cgagctgcgc cacgtcatgg
      181 gactgcttac catggcggac gagggagagg ggagggacga aggtgcggac ctcaccggta
      241 cgacggcggt gccggcccca caacccggcc tggaccagct ggaagcgctg gtcggacgag
      301 tccgggacac cggactgccc gtcggtctga ccgtgaccgg ctccccccgt cccctcccgc
      361 ccggagtcga gctcgccgcc taccgcgtgg tccaggaagc tctgaccaat acggtgaagc
      421 acgcgtccgg cagcgccgcc gccgtcaccg tcgagtacgg cccagaacgg ctccgggtgg
      481 aagttaccga caccggcgga cgcccgggcg cgggccccgg aagcggccgg ggcctgatcg
      541 gcctgcgcga gcgcctcgcc gtctacgacg gaatcctgaa cacgggccga cgtctgaccg
      601 gcggctaccg tgtggaggcc ctgatcccct tggagacacc gtgaccgagc cgctgcgcgt
      661 cctcctcgcc gacgaccaga ccctggtccg caccggattc cggctgatcc tcagggcgga
      721 cggcatcgac gtcgtggccg aggcgaccaa cggagccgaa gcggtcgacg cggtccgccg
      781 tacacgtccc gacgtggtcc tgatggacgt ccggatgccc gagatggacg gcctggaggc
      841 cacccgccgc atcctcaccg gtaccgccga cgcaccccgc gtcatcatcc tgaccacctt
      901 cgacctcgac cggtacgtct atgcggcact gtccgccggg gccagcggct tcctcctcaa
      961 ggacgtcacc cccgagcaac tgaccgcagc gatccgcacg gtccgtaccg gcgacgcgct
     1021 cctcgcgccc gccatcaccc gccgcctcgt gcagcggttc acccggcgcg gcagcgagac
     1081 cgccgccctc caccgtgacc tcacctcgct caccccacgt gaactggagg tcttcggtct
     1141 gctggcccga gggctgagca acgccgaact cgccacccgc ctccacctcg ccgaggcgac
     1201 cgtcaagacg cacgtcgccc gcatcctggc caagctcggc ctccgtgacc gtgtccaggc
     1261 cgtcatcgtc gcctacgaaa caggcctggt cagcgtcggc gcggacgagg ccgcccggca
     1321 gtccgccgag gagacgtagg ccctgctcgg cgccgaggcg gccgacgccc cgatggaccg
     1381 cgctccccgg caccacaccc gctgaggccc ggcttcacac cacccggatc acatgcttgc
     1441 cgcgcacccc gcccgcctcc agcgcgcggt gggcggcggc gatgtccgcg agcgaatgaa
     1501 ccgtgtcgac gacgggccgc aggtcaccgc gctccacgta cgcggtcagc tcggcgaaca
     1561 ggtcatgctt cgggttgcca ctgaagaagc gcacccgccg gcttccgtgg acggctgagc
     1621 ccaggatgta gccgagacct ctgaccgggt ggtggatgtc gaacgcgatg gagaccaggc
     1681 ggccacccgg cgccaacaga cgtctcaggg tgcggtgttc gctgccgacg gtgtccatga
     1741 tgacgtcgta cggacccagc tccgacagct ccaccgagcg atgatccagc accacgtccg
     1801 cccccaactc ccgtacaaaa tcgaggctct tcgcccccgc gaggccggtg acatggcccc
     1861 ccaggacctt cccgacctga accgcgacgc tgccgacacc accggaggcc ccccggacga
     1921 ggaggcgctc acccgcccgg agccgggcct tgtcgcgcag ggcggtgagg gaggtggtgc
     1981 ccccggcggg gagcgaggcg gcctcggtca gggtgacgtt ctcgggggcg tacgcgatct
     2041 ggcgcgggcg gacggacacg tactcggccg cactgccgaa gccgccccgc ccgcgcggca
     2101 ggatgcccca cacccggtcg cccacactaa gcccggtgac ggaggggccc acctcggcga
     2161 cctcaccggc gaagtcgatc ccagccctct ggggaaaccg acgcccggtg acgagccgga
     2221 ccttcccggc ccgcccgtac agctcaccgc cgttgacgct ggccgcatgg acccgcacga
     2281 gcacctcacc cggtccacgt acgggcaccg ggacccgtcc ctcgtagagg acctcgggcg
     2341 gtccgtagct gtcgtagagc gccgcgcgca tgtcgttcat cgggggccgt cctgtgcctg
     2401 ttccgtcggg tgttcctgct cccgacccag gctagacagc taaacggaga aacccctcca
     2461 cttaccctaa agtgagagac atgcctgctc agccctctca caacccgccc ccgaccggct
     2521 ccgaggccct gcgcgccgaa gctctgcgca ccagcgcctt gcgcaccgac gccctgcgcg
     2581 cagacgcccg ggagaaccgc gaccgcatcc tgcgcgcggc ccgcgaggcg tacgcgctgc
     2641 acgggatcga cgtaccgatc agcgcgatcg cccggcgggc cggggtcggg gtggcaacgc
     2701 tctaccggcg cttccccacc cgggcctccc tgatcacgga ggcattcgcc gagcagctcg
     2761 ccgagtgcgc gggcgcgctc gacgaggcgc tggaagaccc ggacccgtgg cgcgcgttct
     2821 gctcggtact cacgaaggtc tgcgcgatgc aggccgccga ccggggcttc acccacgcgt
     2881 tcctggcccg cttcccggac gaatcgagtt acgccgggga gcgcgaccgg gcggaggaag
     2941 gcctggccct gctggtacgg cgggccaagg aggcgggagc cctacgcccg gacttcgacg
     3001 tgtccgatgt ggttctggcc ctgctggcga acagcggggt ggtgaacggg gtccagcccg
     3061 aagcgggggc gacatcacgg cggttggtgg gctacttgct tgagtcgttc cggacggaag
     3121 gagccgtacg acccctgccc ccgcctgtac ggctggagtt ggaccagctt tacggggcgg
     3181 ggtgaggggg ccaacttcga tcggggtcgc cgggaggacc gtagtgtgcc caccggggtg
     3241 gacagcgcgt ccacgatcgg aacaatggga ctgcgcggcc tgcgccgcgg ccacccatga
     3301 agcgaggccg atacgtccgt ttcccgggaa gtgatgaaaa acaacctcct ggccacgcaa
     3361 agccgcaggt caggaactgt ggtccccgcg cgtgcggggg tggtccccag gtgcggaggg
     3421 ttcggaggcg gggcaggtcg tggtccccgc gcgtgcgggg gtggtcccag acggatcgtg
     3481 cgccgcagca gccccttcca gtagtccccg cgcgtgcggg gatcgcccca tcgcgtcgac
     3541 ctcgctgagt cgccgactgg ctcactggct gaggaaccgg gtcgactggg ccccccgaat
     3601 gatgaggcgg accggcagtc tgaagcggca ggtggccgcg gcgaggcccg tcaccccgcc
     3661 caccgccccg taagaaacgc cagggccacc agcgcactca ccggcaccgt cacgcacagg
     3721 tacaccgtcc ggaaccacgg gcggcgcagg ctcagggccg cgaggccgat ccacaggggc
     3781 caccacagca gcgtggcgcg ggggatggag acgtaccagt acgacgttcc cagcgcccag
     3841 aggctcagcg cgacgtacac cgcctccggc cagcggcggt gccgtaccag cagcgcgacc
     3901 agggccaggc ccaccagcat cgccgccaac tccgcctgga acatggccgc gtagccggtc
     3961 gtgtgtgcgc cttcgaacgc cgcgttccac gtgttgctcc acgcctccca cggggtgtgg
     4021 aagtcccggt accagccccg ctcctgtgcg tgcttccacg ccatccagtc gcccgtgtgg
     4081 gcgtgcagat accagctgta cgccagtggc ggcagcgcgg gcagtgccag ccagggcgcc
     4141 ccgcgccagc ctttgcccga ccaggccgag agcgcgaaca gcaccgcgat cgcggccgcc
     4201 aggaacaggc cgctcacgcg caccgtcgtc gccagggccg tcagcgcgca cgccggcgcc
     4261 caacggtggc gctgggcggc cagccaggcc gggagcgcga acgccaggaa cagcgcctcc
     4321 gtgtagccga ccgccaggaa gaccgcgcac ggggagagca ggaagaacag ggccgtgcgc
     4381 cggccgtctg tgtcctcgga gacgtaggag ctggccaggc gggacagggc cagtacggct
     4441 gccgcccccg cgaccagcga gatcagcagg cccgccgccg tccagctcgg gatgacgacg
     4501 tgcaccgccc gcaggagcag ggggaatccg gggaagaacg cctccctgtt gtcccaacca
     4561 tccatccctg ggcctgtgcc ggccgggaag tagccgtcgc gcgctatgtg gaggtagtgg
     4621 ttcgcgtccc actgctggaa cggtgcgagg accggacctg cctcccgtac gtccgagcct
     4681 cgggggaaga gccaacgggc gccatgggcc gtgatccaca cggatattcg ggtcagcagg
     4741 tagagccaga gcacccggcg gtcggcggga ctcagccagg cggccgcccg gtcgaaccct
     4801 gccgcgcgaa gcgcgcgtgg cgcaggccgg gggtggtgcg gtacgggctt ctcggccggc
     4861 cggaactcat gcgttacggg ccgggactcg gggcccctcg gccgtggcgg ggcaggcctg
     4921 agccccgggg gcaacgtaga cactcaagca cctttcggac acgtctgggc gtgcgagatt
     4981 gcccggtgga gacgccgggg catgggaggg cagggcgagg gcgcggagtc agccgcccac
     5041 catcacggca tcgtcgtcgc cccgagcgtc gatgtagacg tcgatgtcga cgaagcactc
     5101 gtcggtgcct ccgtcgtcgg caggcccccc ggagcggtcg tccccgtgac ggcaccctcc
     5161 gaccccggca ccgtattgct cggcgtgggc ggttccgtcg gcgtccccgc gggtggcggc
     5221 ggtgccgggg acgggtagct gggcgacggg ctcgtcgccg gaggtgcggg cttcggcgtg
     5281 accgggagca ggccgaccgc cgccgcgccg cccccgccga tgaccaggca cacccccacc
     5341 gcgaccgcgg ccatccggcg ccgctgggcc cgcatgccgc gttcggtgat gtacgtggcc
     5401 ggctccggga ccacgtggcc ctggccgaag tccccggcct gccggaacag ggaccgcaac
     5461 ggatcctgcg cgccccggga cgcctccggg cccgaccggt cccgtggttc agacatcagg
     5521 agcctcctcc atgcgggggt cctgcagacg gtgggcgagc gccgcccggc cgcggaccag
     5581 atgcgtcttg atcgtgctgg cggaaagccc ggtctccccg gcgatctgct ccaccgtgag
     5641 gtcgcacagg tagtgcaggg tcaacgtccg ccgctgctgg gcgggcagtt cgcggagcgc
     5701 gtcgacgagc accacgtggt ccgggtccgg cggcgcgacg tgttccgccg cgccgctccc
     5761 ccggccccag gcgtccgccg aacgccgccg gaagcgccag cggctcaccg ccaggcgcca
     5821 cgcgaccgta cggatccacg cctcgggctg cccgtcccgc tccagccggc cgcgccggac
     5881 ccacgccttg acgaaggcct cctgcacgac gtcctgcgcc tcctggaggt cccccgtcat
     5941 cacgtacagc tggcctgtga gacgcgtgac cgcctgggcg tagaactctt cgaactcctc
     6001 gacggtcaaa agttgctccc ggatctttcg cttcaccggg tatacgcccg ccgccgggca
     6061 tccggtctac agccccggcg gtccatcgga gtgatcgtcg tcacgtgacc tggcacggac
     6121 tgcatcgagg gccggcggcc atcgccgtca gccggtcaca ccgggtgcgg cagatccatg
     6181 agcgcgagcc gggcggggtc gatcagtgcg gtgatcccgg tgatacggcc gtcggcgacg
     6241 gtgaaggcga ggagggaggg cggggtgccg tcctcgctcc aggagatgac accgggcagt
     6301 ccgccgacgg tcaccgtgtg ttctcgtgcc gcgctgctgg ccgcccgtgc gcgggtggcg
     6361 acttcggtgg ctccgagcgt cacgaaccgg ccgttcgggg agtggacggt gaacgtcacc
     6421 tcagggtgga gttcgggcag gtcgccgtcc gactccgccc gccggaacga cagcggccga
     6481 agccgcgtag taactggggt tcttcatgcc gcgatcctgc cgcacaacag ttcccacctg
     6541 cgccggcccc ggctcctccc ggagctcaag ccgcgagatc cgagcgttct ttacggtgat
     6601 ggggtggatg ggtcactttt cccacccacc caccctgcga gcgaagttga ccttttgcga
     6661 ccgacttgcc acgcacagtc gccgcgcttt agcgtgcgga gaacgagacc ggaaaaagcg
     6721 acccctgctg gtgcttcaac accgaacagg ggtctgacca acgaagatcg gaacaatcga
     6781 tccctatggc tgacagccac cctaatgctg cctcgcgccc cccgcacgag gccccgccca
     6841 cgcacccgcc cgcgactccg cacccgatgg ccaaacccgg ctacggcaag cgctccgcac
     6901 ccggcgaacg accgcgcacc gaccgcgact tcgcccacct gctcccccgt gacgccgccg
     6961 tcgccgcgta tatcgaccgg ctgcccgacg gctccgacat ctccgtgaag acgctggcca
     7021 agcacctccc gtacgggcag tgcgcgctga gcaccaccct caaccggctt caggacgccg
     7081 ggcatctgcg gcgcggcagc gagtgcgtga tcaccgacga ggtgatgttc tgggtgaccc
     7141 ggaccttctt ctcgcgcacc gcacgcgagc acgagtggtg gaacgcctac atccggggcg
     7201 acgtcccgcc ggaccggccc caacgaccca cccgctcccg ggcgttcatc ctgctcgccg
     7261 ccctcggccg tacggctccg gcgctgtccc tctccgccgc cgactgcgta cggctggaac
     7321 cgcaggtcag cgaatggttc gcgcggggtg cgacggaggc cgaactgctg cacgccctca
     7381 ccgcgggcct cccgtccccc gtccactgcg ccgcccgcct cgtcgagcgc cgcctgcgcg
     7441 acaagctgcc acccgagcgg aacgtgccag ggccccggac cgttctgcgc accctggagt
     7501 gcggccagtg cggagtcccg ggcagcccgg aagccctgct cggcgggctg tgccggaggt
     7561 gccgtgggcc ggtgcccgag ctgccgatcg gcccgccggg agccgtgcag ccgcctgggg
     7621 tacgggagcg gatggacgcg gtccgggcgg ggctggttcc ccgatccgag aggcagcatc
     7681 aatgattccc gtccaggact tcgaggcgct ggcccgcgtc tgccccgaga ccgtgacgct
     7741 tgagctcatc aacggaaagc tagaggtcaa gcccgtgccg gacggggacc acgacgagat
     7801 catcatgtgg gtcgcgatgc agtgcgtacg gcagcggccg gagctgcggc tgtaccgcac
     7861 acgcgggctg cggatcggcg gcgaccgcaa cgaccgcgcc cggccggacg gcagcctggc
     7921 ccgcgcgggc cgcttcgccg gggacggcgc gtggtcgagc ccggaccgca tcctcatgac
     7981 tgtggacgtg acctcgtacg acgacagccc tgtgcagccg tgcggctacg cggcggcggg
     8041 catcccgttg cgcctcctca tcgaccgccg gtccggcact ttcgccgtcc gcagcgaacc
     8101 ccactcggct cgctaccggc atcagcgcct gtacgacatc ggcgaagccg tcgccctccc
     8161 ccgccccgcc tccatcaccc tggacaccga ggagctgaag aactacaccg gctgatccgc
     8221 tgcggccgcc agctgcgtcg tcgccagttc gcggtacagg tcgtccgacg cgatcagctc
     8281 ctcgtgcgtg cccgccgtgc ggacgcggcc ctcctccagg accacgatgc ggtcggcgtg
     8341 gcggacggtg gacaggcggt gggcgatgac caacaccgtc gtctgctcag ccagttcgag
     8401 gatgacgtcg cgcagcgcct gctcgttgac cgcgtccagc tgcgaggtca cctcgtccag
     8461 caggagcagg cgggggcggc gcagcagggc gcgggcgatg gcgatgcgct ggcgttcgcc
     8521 gccggagagg gtgacgccgc ggtggcccac cgccgtgtcc agaccgtcgg gcaggcgggc
     8581 gaccagggcg tccaggcggg tccgggccac cgcggccgcc agccgggcct ccggggcgtc
     8641 cggggccgcg aagagcaggt tctccctcag cgtcccggcc aggaccgggg cgtcctgctc
     8701 cacgtacgcg agcgaggcgc gcagctccgt cagcggccag tcccggatgt cccggccgtc
     8761 cacggtgatc gtgccgccgt cgtggtcgta gaaccgttcc agcaggctga acaccgtcga
     8821 cttccccgcc cccgacgggc ccaccagcgc caccatcccg ccggccggga cctcgaacgt
     8881 gacgcccttg tgggcggggg cgcgctcgtc cccgtacccg aaggtcacgt cctggaaggt
     8941 gacccgcagc gggccgccgc cctcctcctt caacccgccg aattcctcgg ccggttcgcc
     9001 cggcagtgac tccacctcgt cgatcctgcg tacggcggcc aacccgctct gcaggcccgt
     9061 ccagccctcg accaggccgc cgatcgggcc catcaggtag aaaaggtaga gcaggaacgc
     9121 gatcagggag gagatgtcga gcgagcccga cgccacccgc gcgccgccca ctcccagcac
     9181 ggccaggaag gccagttgga tcgacatcat catcgtgacg tcggagaccg aggaccactt
     9241 cgccaccgag acgccccggt cgtgcgcgtg ccgtgccgcc tccgcgaccg ctgccgtctc
     9301 ccgctcctcg gcgccgctcg ccttgaccgt acggaacgcc tggagcaccc ggtccagcgc
     9361 cgcgcccatc gcaccgaccg actcctgtgc gcggagctgc gcgcgctgaa tccggggcat
     9421 gagcagagca gttacggctc cgataccgac gatcaccacc agcgtcacgc ccagcagcgt
     9481 gacgtccatg tacgccatga acgcgaccgt tcccagcagc atcagcacgc tgttgaacga
     9541 ctcgaccagg ccgctggaga ggaccgttcg gaggagggtc gtgtcgctgg tgacgcggga
     9601 ctggaggtcg ccgggggtca gacggtccac cgccgggacc ttcaaccgca tgatccggcc
     9661 caccagttgg tggcgggctc ggaggacgac gccctctccc gtacgggcca tgaggtaacg
     9721 gccgtacgcg gagaggcagg cgcccgcgag gaccagtgcg gtgagagcta cgagcggggc
     9781 gacgggcgag ctgcctgcgg cgaacgcgtc caccgcgtac ttcgccatca gcggcatcgc
     9841 caggccggcc gcggaaccgg tgagcgagag caggcccgcg cggatgagta cgcctcggtg
     9901 ggggcggacc cgggtcagga gaaggcggag aggggacggg ccggagcggg ccggggcctc
     9961 cgcccctggc tccttcgcgt cctcaggcgc ccccatgccc tcagggcgcc tgtccgcccc
    10021 cgtcgtcgtt cccgccaccg cgctcacccg gaaccccctc gcgtaccgtc accaccatca
    10081 aaggacgcag atcggccgct tccgcctgct cgccgaccgt gcggccctcc gcctccaccc
    10141 aagcatgcgg cgagaacggc ggtgccgtcc ggacccccgc gcaccagtcg ggccagctcc
    10201 ccgacatccg gcaggccagc gcgatggcca ccgaacgggg caggcagccg taacggcccg
    10261 cgcagcggcg gctggtggcg gtgaccgcct cgtacatctc cagcgtctcg tcgtacgtcg
    10321 ccgggttcgc gcccctgctg aggcgcgtca gcacccggcg caggcggccc ggcttgaagc
    10381 gggccaggac gaacgcggcg gcgatcgcgg tacgcagccg gaggccgccc gggcccgcgc
    10441 cccggcgggg catcgtcatc tcggtggtca tgggcgcagg atcttcgcct cgacgagctg
    10501 acgggtcagc tgggcgacgt ccgactccgc ctccgcgcgc tcgacgtcga actcggcgac
    10561 gatgcgttcg acggccgctt caggggcctc gccggccgcc agggtctcga ccacgatcac
    10621 gctggtgtcg ttcaactgcc agtagctgcc gtccttttcg tcgagcagga cgcctccgta
    10681 ctcggtcgtc gtgacggcta tgccttttct gagcttcatc gcgctgcctc gctcggggag
    10741 atggtcgtgg gggtccggtc gggtgggggc caggcgttct cggccgtgcg cagccaggtc
    10801 tccccggcca cggtggagct gatggagtgc tcgaccagga ccggcgagaa gggttcggcc
    10861 gccagtcgga gcaggtggcg ggcgtccacc aggccgtgtt cggccaggcg cgagccggtc
    10921 cacaagtcgg ccagttccga ggcgtgttcg cgtaggccct ggtgttcgtc ggaggacatg
    10981 tggtccttcg tcgtacgggc cagcagcgcg tccggcacca ccccgcgcat cgcctcgacg
    11041 agcagcggct tgtagcgcca ggggctgatg cgctcctcca ggcgtacggc gagggtcgcc
    11101 tccaggaccc ggtcgtcgta gaagggcgcg gcgaccggca gcccgatcgt catgccgacg
    11161 tcctccatcg cctggaagtg ccgcgccccc atccgtaccg cgtcgatgtc cacatgcctc
    11221 ccccgccacc ggtcgatcgg ctcggcctgc tcggcggccg cccggaactc ctcggtgatg
    11281 agggcgttcg cgtggtccgt gaaccacggg cgcagggact ggcgtacacc ccaggagagc
    11341 agcggggtgc gggtgacggg ctggggttcg gtggtgaccg cgcccgcgag ccagcggggg
    11401 aaggtggagc ggttcagcag ggccttcagg gtggggagga ggggccagcc gagctggtgg
    11461 cgcaggccgc tgaggtggtt ccaggcggtg agcgggttgc cgtggaagag gtcctggtag
    11521 gcgtggggca ggccgaggaa gagttcgtcg ccgccgtagc cggtgaggtg gtagcgggag
    11581 ccggtggcgt gggcgcggcc gaggagcagg tgcgcgcggg cgcggccggg ggccacgggg
    11641 agcggttcgt cgttgagctc ggcggaggtg gcggccaagt cggcgtagaa gtagggggct
    11701 tggtcggcgg ggatgatgtg gtgggccagg gccggggtgt ccgtatgccc gccgcgtgcc
    11761 gcctccaccg cccgccgcgc ccacgtctcg tcctcgctgt aacggtcccg ggccgccacg
    11821 gtgagcagcg acagctccgc cggggcggtc gcgcgggcgg cgaaggtgag ggtggtggag
    11881 tccaggccgc cggagagctc gcagctgatc cggtccaggc cgccgatatg cgctcgtacg
    11941 gaggcggcga cggcgtcccc gagtcttcgg gcgccctcgg ccagggggag ttccggcggc
    12001 gggggctccc accagcggta ctcgcgaggg ccgggggcgg cggccgtgcc cggtgctgcg
    12061 gcgggcaggg ccagtccgta cccggccccg gtctcgtgca cgccccgcca caggacgcgc
    12121 cggctcaggg ggtgcggcag gaagtccagc atgcgcaggg cgagcgcgcc gtcgtccagg
    12181 ggtgcgccgg tcaggtgcgc gaggacggcc gggcggtccg aggcgatggt tccggcctcg
    12241 gcgggccggg cgtggtagat ccggcgcagg ccggaggcgg tgccccggat ccaggtctcg
    12301 ccggagaggt acgcgatgac gtgatggagg ccggggagcc gggcgaggcg ccgttccagc
    12361 ccggcccggt cgcgggcccc ctccaggagg ccggcgagca cggcctcggg gacctggtcg
    12421 gggccgatga gcacgagggc gtcgtcgccc cggcggaggt ggctgacctt gcggaccagg
    12481 gggcgggcga cgacccaggg gcgtccggag gggtgggcga cggtgacggt gccgcccgcc
    12541 cctcctgcgg tccgggtggt gtccggtacg ggaagctgcc cggccaccgc gtggcccgcc
    12601 acgctgtccg gcaggacgac gaagtagtca cgcagcaggc cgggcatctt cacctttcgg
    12661 aagttctcac accgcgcaca gggagccggt cggcggatca gatgatgcgc cggacccagt
    12721 actccttgta cgagccgtag aagtaaccgg cggtcttctt cgaaaagtcg ccctgcttga
    12781 acagcgacgg cttcacgtaa gccttcttct gctgcttcat gacaccttcc cttctgtgcg
    12841 gttgacgacc gttatgccca actgatgtct gcataactgg agttattcat gcgacaggtg
    12901 ttcaccgggt gaacaccagg gccatcatgt cccgccgccg ggccggtacg cgtgattccg
    12961 ggccaccggg tgaagttggc cggatagcgc gagcgaagag ggcgggcgcc gcggccctcg
    13021 gccgcaccgc agggcaccga ccggtcggca tgtgcagtcg cacaccgacg cggcacgcag
    13081 gagcagtgga ctggaccaat ggctgtacgc ggcgaccggc ccgggcagtg agctgccaag
    13141 agatcgaacc ggcggcggcg gaacgaggag accaggccgt acgcccgctc ctgggttccg
    13201 cagcggccct atcccaccgg cagcgcgtgc gccaccggga tcaccgaaac cgatcgaaca
    13261 cgcgttgccg tacaggaccg gacacggcaa gtagccattc ggccatgccc gaccgctcgt
    13321 tcgggtccat cagggcctct cggcctccct cgacgccccc gccggcgcct ctcaacgccc
    13381 cagccagcac ccctcagcgg ccccgacgcc ccgcctcgcg catccggtag ccgccccagg
    13441 ccgccgttcc ggcgcccagc agcagcggga tcatgccgta gccgacgacc gtcgacgagt
    13501 gggcgaacca ccgtccaccg tcggtcacct gcgtcgcggt gatcgtctcg gggttgctcc
    13561 ttccgtcgtg gtccgcgaag aggatccggg tgcgctcgat cctggccgac tcgggcatgc
    13621 cgtccacatg ggcacgcaga gcctcatcgg cgacccgctt gcgctccgcc tcgttggcgc
    13681 gctgctgcgc tggggtctcg ccgaagcagt gccagaccgt ctgccccttc tccgtacgcg
    13741 tgtcccggca gtcgatccgg ccgaaggtct ccacccgcat gccgagcagg ccggagagcc
    13801 ccgtgccggt caccacgatg aacagccccg ccgcgaacag cacggccccg cccaccacga
    13861 gcgaggcccc cttgagccgc cctccgcccc ttctccccgc agcgctcacg ctcatgcccg
    13921 ccccgccccg tatccgtacg accgccctcg cggtgtccac gaccgccccc gcagtgtccc
    13981 cgcacgccct ggacacgcca caggggcggc acctccgtga aggaggtacc gcccctgtgg
    14041 gacgtgcggg gagcttcgac cggttccgaa ggagccgatc agaagtccat gtcaccgccc
    14101 ggcatgccac ccggggcggc cgcgccggcc ttctcgggct tgtcggcgat gacggcctcg
    14161 gtggtgagga acagcgcggc gatggaggcg gcgttctgca gagcggagcg cgtgaccttc
    14221 gccgggtcga tgatgccctc ggcgatcatg tccacgtact cgccggacgc ggcgttgagg
    14281 ccgtgaccga tcggcaggtt gcggaccttc tcgacgacga cgccaccctc gagaccaccg
    14341 ttgacggcga tctgcttgag cggggcctcc agcgcgagct tcacggcgtt ggcgccggtc
    14401 gcctcgtcgc ccgtgaggtc gagcttgtcg aagacggccg aggcctggag cagagccacg
    14461 ccaccaccgg cgacgatgcc ctcctcgacg gcggccttgg cgttgcgcac cgcgtcctcg
    14521 atgcggtgct tgcgctcctt gagctccacc tcggtggcgg cgccggcctt gatgacggcc
    14581 acgccgccgg ccagcttcgc gaggcgctcc tggagcttct cgcggtcgta gtccgagtcg
    14641 gagttctcga tctcggcgcg gatctgcttg acgcgaccct gcacctgctc gctgtcaccg
    14701 gcaccgtcga cgatcgtggt ctcgtccttg gtgatgacga ccttgcgggc gcggccgagc
    14761 aggtccaggc cggcgctctc cagcttgaga ccgacctcct cggagatcac ggtgccaccg
    14821 gtgaggatgg cgatgtccgc gagcatggcc ttgcggcggt cgccgaagcc cggggccttg
    14881 acggcgacgg acttgaacgt gcccttgatc ttgttgacga ccagggtgga gagcgcctcg
    14941 ccctcgacgt cctcggcgat gatcagcagg ggcttgcccg actgcatgac cttctccagc
    15001 agcgggatca ggtccttgac gctggcgatc ttcgagttga cgatcaggat gtacgggtcg
    15061 tcgagcgacg cctccatacg ctccatgtcg gtcgcgaagt aggccgagat gtagcccttg
    15121 tcgaagcgca taccctcggt gagctcaagc tccagaccga aggtctggga ctcctcgacg
    15181 gtgatgacgc cttccttgcc gaccttgtcc atcgcctcgg cgatcttggc gccgatctcg
    15241 gtgtcggcgg cggagatgga ggcggtcgaa gcgatctgct ccttggtctc cacgtccttg
    15301 gcctgctcca gcagagcggc ggagacggcc tcgacggcct tctcgatgcc ccgcttgagg
    15361 gccatcgggt tggcgcccgc ggcgacgttg cgcagaccct cgcggacgag agcctgggcg
    15421 agaacggtgg cggtggtcgt accgtcgccg gcgacgtcgt ccgtcttctt ggcgacctcc
    15481 ttgaccagct ccgcaccgat cttctcgtac gggtcctcga gctcgatctc cttggcgatg
    15541 gaaacaccat cgttggtgat cgtgggcgcg ccccacttct tctcgaggac gacgttacgg
    15601 cccttggggc cgagggtgac cttgacggcg tcggcgagct ggttcatccc gcgctcgaga
    15661 ccgcgccgtg cctcctcgtc gaacgcgatg atcttggcca tgtgaagtgg tcctcccgga
    15721 caggggtgga ttctccggac cgagaggcgc ccgcgacgga cggcctgcgt gcgtggtggt
    15781 tccttgcccc accgcgcctg cgggcctcac cggcccggtc caagtttctg tcactctcac
    15841 ctggagagtg ctaacgccaa tgattagcac tcgacccccg agagtgcaag cgcctcaggc
    15901 cagggcccgc catgtggtca ggctgcccgt ccggccatcc gtccgcccgg tcccggcgcg
    15961 cacgaagggc ccgatcccct ggatcactcc ggggtcgggc cctgcgtgcg tgagtagtgg
    16021 tgatgccgga cggccgcgcc ggactcagcc gacagcgagc ttgaccatgt cggcctgggg
    16081 ccccttctgg ccctgcgaga tctcgaattc aactcgctga ccctcttcga gggtgcggta
    16141 cccgtccatc tggatcgcgc tgtagtggac gaaaacatcc gcaccaccgt cgaccgcgat
    16201 gaagccgtac cccttctccg cgttgaacca cttgacggtg ccctgagcca tgcctaactc
    16261 ccctataact ggcccttgca cgggaccgca cttcgcggcc ccgggtcgga actcaccctc
    16321 cgacaggaga gggtgcgcgg cgccgcagcg cgtcgaccgc ggccgaatgt atccgcccaa
    16381 ctgccctctg caacaggtca atcggacgag aattctgggc acaggcgaac gcccgaatat
    16441 gcgggattgc tgaaatttac gggcaagtcg ggccaggcaa aggccactta tgcctcaagg
    16501 ggttcacgta ctttggctgc ttcttatcgg ggccgggcgc attctcatat gcgggcggca
    16561 cacgcagcga agggggcttc cccaactcta ccgtgctcaa ccatgcagaa ttgccccctc
    16621 cgctctgtgt ggcggagggg gcaactgttg ttcagccgag ggcggtcggc ggttcagccg
    16681 ggggccgtcg gatcagccgg ggtcgtcgga tcagcagccg ccggccacgg ccgggatgat
    16741 cgagatgccg gcgccgtcgg gcgtggccgc gtcgagcccg ccctcgaagc gcacgtcgtc
    16801 gtcgttgacg tacacgttga cgaagcggcg cagcttgccc tggtcgtcca ggacgcgggc
    16861 ggcgatgccc gggtggtcct tctccaggga gtcgatgacc tcggagagct tcgcgccctc
    16921 cgccgtgacc tcggcctggc cgcccgtgta cgtacggagg atggtgggga tgcggacctt
    16981 gacgctcatg gtggtgccct tcttccttcg ttctggggtc aggcggaggc gaggcccgcg
    17041 gcgcggaacg cgtccaggct cggcttgatg gtcgccgtcg cctgcgaggt cgcggagacc
    17101 gcgtcgagcg tcttgagccc gtcaccggtg ttcaggacga cggtggtgag cgccgggtcg
    17161 atgacgccgg cctcgatcag cttccgcgtc acgccgagcg tcacgccgcc cgccgtctcg
    17221 ccgaagatgc cctcggtgcg cgccagcagc ttgatcgcgt cgacgacctg ctcgtcgttc
    17281 acgtcctcca ccgcgccgcc cgtgcggcgg gcgatgtcga ggacgtacgg gccgtccgcc
    17341 gggttgccga tggccagcga cttggcgatg gtgttcggct tctgggggcg gaccacgtcg
    17401 tgaccggcct tgaaggcggc ggagaccggg gagcagccct cggcctgggc gccgaagatc
    17461 ttgtacggct tgtcctcgac caggccgagc ttgatcagct cctggaaccc cttgtcgatc
    17521 ttcgtcagct gggagccgga cgcgatcggg atgacgatct ggtcgggcag cttccagccg
    17581 agctgctcgc agatctcgta cgccagggtc ttggagccct cgccgtagta cgggcgcagg
    17641 ttgacgttga cgaagcccca gccctcgccg agcgggtcgc cgatgagctc ggagcagaag
    17701 cggttgacgt cgtcgtagtt gccctcgatg ccgaccagct caccgccgta caccgcggcc
    17761 atgacgacct tgccctgctc caggtcgtgc gggatgaaca cgcacgagcg cagtccggcg
    17821 cgggccgcgg cggcgccgac ggcaccggcc aggttgcccg tggaggagca ggagagggtg
    17881 gtgaaaccga aggcgcgggc ggcctcgacg gcgatcgcga cgacgcggtc cttgaaggag
    17941 tgcgtcgggt tgccggagtc gtccttgacg tacagaccgc cggtgacgcc cagctcacgg
    18001 gcgaggttgt cggccttgac cagcttggtg aaaccggggt tgatattggg cttctccgcg
    18061 acatcggccg gaaccggcag cagcggggcg taacgccaga tgttgttcgg gccggcctcg
    18121 atgcgcttgc gcagttcctc gggggacccg gtgggcaggt cgtacgcgac ttccaggggc
    18181 ccgaaacagg acgcacaggc gaaaaggggg ccgagctcga aacgctcgcc gcactcgcgg
    18241 caggaaagcg ccacggcggg accgaggtcc acggaagcgg tggtggcatt acctgcgatg
    18301 gtctgaacag ccatgatggc ggaggccctt tctcctcatc ttccttgcag cgcatttcgc
    18361 cacaagacgg aattggcacc ttccccaccg tgacctcgcg gtcggtagga gggttgccgg
    18421 gacttcaacg ggccgttccc tcagtccctc tggatgagct ctgtggcact ggatcttcga
    18481 tccaggcgtt tgtgtgacga cggaccccga catgcgacgg tcatccgcgt tgttcaagac
    18541 tgtaaccgaa ggaccggact gttgagatag tcgtccgaac ggcgagatgg atcacatacc
    18601 gcccccacac gaggagtccc gaccgtgctc gaagaggtgg aacgctggct gaccaggcgt
    18661 tcctggtcgt ccgccgaccg cccgctgaac cggctcaccg acgcccgggc cgccgaccct
    18721 caccgtacgt cggtgagtgt ggtgctgccc gcgctgaacg aggaggccac ggtcggcgcg
    18781 atcgtggcga ccatccggcg ggagctgatg gagaaggtcc ggctggtcga cgagctggtc
    18841 gtgatcgact ccggctccac cgacgccacc gccgccgtcg cccgcgcggc gggcgcccgg
    18901 gtggtccacc gggacgcgat cctgccccgg atcccggccc tgcccggcaa gggcgaggtg
    18961 ctctggcggt cgctcctggt gaccagcggc gagatcgtct gcttcatcga cgccgatctg
    19021 aaggacttct cggcggactt cgtctcggga acggtcgggc cgctgctcac cgatcccgcc
    19081 gtgcagttcg tcaaggcgat gtacgaccgc ccgttcggcg acaccgcagg tcagggcggc
    19141 cgcgtcaccg agttggtggc acgccccctg ctcaatctgc actggccgca gctggccggc
    19201 ttcgtgcagc cgctgggcgg cgagtacgcg gtgcggcgct cgctgctgga gcggctgccg
    19261 ttcccggtcg gttacggagt ggagctgggg ctcctcgtcg acgcgctgca caccgtgggc
    19321 ctggacgcgc tgggccaggt ggatgtgggg gtgcggcgcc accgccacca ggacgggcag
    19381 gcgctgggcc ggatggcggc ggcgatctac cgcacggcgc agctgcggct ctcccggggc
    19441 catctggtgc ggccggcgct gacgcagttc gagcggggcg cggaggggtt cgtaccgcgc
    19501 acgcacgtgg tggacacgga ggagcggccg ccgatgcggg agatcgagga gtacgtggag
    19561 cggtgggcgg cgtgaggtgc ggggggctcc gcgccgtggc gggcgtgagg tgcgggggct
    19621 ccgcgccgtg gcgggcgtga ggctctgagg cgtttggcgg cttcccttac gggctaggtt
    19681 ccgctacatg gtcaccgagc acgcaccctc ctccactgcc caggtcctcg tcgcgtccaa
    19741 ccgcggcccg atcacgtaca cgctcggcga ggacgggacg ctcgacgcca agcgcggcgg
    19801 aggcgggctc gtctccgggc tgagcgccgt cgacgacaag ctgtgggtct gcgcggccct
    19861 cggcgacggc gaccgggagg cggtgcggcg cggggtcggt gagccgggcg tacggatgct
    19921 ggacatcgac gccgaggtcc acgccgacgc gtacaacggc atcgcgaact ccgtgctgtg
    19981 gttcgtccac cacctgctct accagacgcc cgtggagccg gtcttcgacg cggagttccg
    20041 gcggcaatgg gcgtcgtacg aggcgtacaa ccgggccttc gcccaggcgc tcgcggagga
    20101 ggcgggggaa ggggcggcgg tcctggtcca ggactaccac ctggccctgg tccccgggat
    20161 gctccgcgag ctgcgcccgg acctgcggat cggccacttc tcgcacaccc cgtgggcgcc
    20221 ggtggactac ttccggctgc tgcccgacga catcggtgag cagctgctcc gcggcatcct
    20281 gggcgcggac cgggccgcgt tcctgacccg gcggtgggcg gacgcgttca tcggctgctg
    20341 tacggagatc ctcggcggga cgggccggac caggatcggg gtgcacgggc tcggggcgga
    20401 cgcggacttt ctgcggcggc ggtcgcagga ggcggacgtc gacgagcgca tggaggcgct
    20461 gcgggagcag gtgggcgagg gccggaagac gatcgtgcgg gtggaccgca ccgagctgtc
    20521 caagaacatc gtgcgcggcc tgcacgcgta ccgggccctg ctggagaccc acccggagtg
    20581 gcgcgagcgc gtggtccacg tcgccttcgc ctacccctcg cgccaggacc tcgcggtcta
    20641 ccgggactac acggcggcgg tgcagaccct cgccaccgag atcaacgggg cgtacggcac
    20701 ggagagttgg acgccggtca tcctccacgt gaacgacgac ttcgcccgct cgctcgccgc
    20761 ctaccggctg gcggacgtcg cgctggtcaa cccgatccgc gacggcatga acctggtcgc
    20821 caaggaggtc cccgtcgtct ccgaccacgg ctgcgcgctg gtgctgtcgc gggaggcggg
    20881 ggcgtacgag gagctgggcg aggacgcgat cgtggtgaac ccgtacgacg tggtgggcac
    20941 ggccgaggcg ctgcacgagg cgctgtcgat gggcggggac gagcgttccg gccgtacgaa
    21001 gcggctggcg gaggcggcga cggcgctccc gccgcagcag tggttcctgg accagctgga
    21061 ggcgctgcgg caggagtgag ggcgccttgg aggcggtcgc cttttagagg cggtccgcca
    21121 gagccgccag gaagtccacc acggcggccg ggcccgggac caacaggtcg gcacgctcgg
    21181 ccagttcggg cacctcggcg ctgccgctgc acaccagcag ccccgggatg ccgtccgggc
    21241 cggcgccgcg cagcttctcc accgcggcga aggcggcgag gtccccgagg tcgtcgcccg
    21301 cgtacaggac ggactccgcg tcgacctcgg cgacgtacgt ggccagggcg acgcccttgt
    21361 ccatgcccgg cggacgcaac tcaaggacct ggcgccccgg ttcgaggatc agcccgtggc
    21421 gggcggccag ctcgccgagg gggccgcgca gggtctcgaa ggccgcctgc gggtcgtcgg
    21481 cacggcgggt gtggacggcg acggcctggc ccttctcctc ggtccaggtg ccgtgccagg
    21541 agtcgaactc gtgcagtacg ccggggagct cggcccgcac ggcggcgact ccggggtgcg
    21601 gggcgggggc gttcaccgtg ccggtggcgg cgtcccagcg ctcggctccg tagtggccga
    21661 ggaccacgag gtggtcgagg ccggggacgc ccgcgaagcc gccgtagcgg acggcgacgc
    21721 ccgcggggcg gccggtgatc acggcgacgg aggccacttt gggcgcgagt gcggcgagcg
    21781 cctcgacggc gccggggtgg gcgcgggcgc gctcggggtc cgggacgatg tcggcgagcg
    21841 tgccgtcgaa gtcgagggcg atgaccgcgc ggtcgggccg ggcgaggatg gcctccaggc
    21901 cctcgcggcc ggccggggtg gtcggagtcg ggatcggcgg ctgtacggag tggctgccca
    21961 tggggtgagc ctagcaatcc agctcctgcg gggctccgcc ccgcaccccg ctcctcaatc
    22021 gccggagggg ctcagaaatg ctccccccag aggacagccg ggggcggcag aatcaagccc
    22081 ctccggcgat tgaggagcgg gggtccgggg gcagcgcccc cgaaaaggct cagcggcgct
    22141 cccgccgcgc cgccttcagt cggcgcagcc ggttcaccgt caccggatcc tcccgcagcg
    22201 cccgctcatc ctcgatcagc gcgttcaaga gctggtagta gcggaccggc gagataccca
    22261 gctcctcccg gatcgcccgc tccttcgccc cgggccccgc ccaggacctc cgctccagcg
    22321 cgagcagcgc acggtcacgg tcggacagcc ccggggcaac aggctcgggc tcggcgtcag
    22381 gcgcggtcat acaaccaacc taaccgcgcc cactcccacc gcgcccgccg cctactccgc
    22441 gctctccgcc ttcgccgcct ccgccgctat cgacccgagc acccgctccg ggctcccgcc
    22501 gggagccacc gcctcgccga tgttcgtctt gatcgactcg ctcaccgtcg cccaggacgt
    22561 cttgccgaag gggggcagct gggagttggg cagcgccgcc aggaactcgc ggagcttcgc
    22621 gtgcttggag tcgatctcca tctcggc
//
