LOCUS       NZ_KB891296            10222 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. ScaeMP-e10 B061DRAFT_scaffold_0.1, whole genome
            shotgun sequence.
ACCESSION   NZ_KB891296
VERSION     NZ_KB891296.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. ScaeMP-e10
  ORGANISM  Streptomyces sp. ScaeMP-e10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1873521)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Improved High-Quality Draft of Streptomyces sp. ScaeMP-e10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1873521)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 1873521)
  AUTHORS   Stepanauskas,R., Sieracki,M., Masland,D., Poulton,N., Swan,B.,
            Garcia,M.M., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C.,
            Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V.,
            Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I.,
            Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X. and
            Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. ScaeMP-e10
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            Isolation Site                    :: Gulf of Mexico
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,185
            CDSs (total)                      :: 7,109
            Genes (coding)                    :: 6,996
            CDSs (with protein)               :: 6,996
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 1 (5S, 16S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 23 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 910883
            Orig. end                         :: 921105
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB891296.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405355
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10222
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10222
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10222
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5222](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5222
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            741..1343
                     /locus_tag="B061_RS0104085"
     CDS             741..1343
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015611031.1"
                     /locus_tag="B061_RS0104085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018510295.1"
                     /transl_table=11
                     /translation="MTRRLHAGRRAATAALGLSLLLALAGCGDDAADADGKASVPEGWS
                     TLDTDSLSVGYPKDQGYAEQSAAERSRSNAAVALKVEDGIRTGMVSVQLNFATGVSDAA
                     EAAAAAGAGIGLGATRKATEDVRLAGPSPARDARRIDYDFTADGKEDTPTRGTPMTGVL
                     VAGVDSGGVPFVVRLNAVREAVDGEDLDAFVGSITVK"
     repeat_region   1691..4340
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=912635..912663
                     /rpt_unit_seq="gtggtccccgcgcaggcgggggtgttccg"
     misc_feature    complement(1924..1938)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.56"
                     /tool="antismash"
     misc_feature    complement(2292..2311)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.19"
                     /tool="antismash"
     misc_feature    complement(2780..2799)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.38"
                     /tool="antismash"
     misc_feature    3189..3207
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.64"
                     /tool="antismash"
     misc_feature    complement(3969..3987)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.85"
                     /tool="antismash"
     misc_feature    4551..4567
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    4558..4573
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.96"
                     /tool="antismash"
     misc_feature    4937..4950
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.1"
                     /tool="antismash"
     misc_feature    complement(4937..4950)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.32"
                     /tool="antismash"
     gene            5001..5222
                     /locus_tag="B061_RS0104090"
     CDS             5001..5222
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676668.1"
                     /locus_tag="B061_RS0104090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018510296.1"
                     /sec_met_domain="strepbact (E-value: 2.5e-22, bitscore:
                     75.9, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVPTQVISDADLDNVSGGILNGSLNNNALGLAADAPVSLS
                     GALQAATGTVDAATGLNLQGVSGLVSGL"
     gene            5403..6212
                     /locus_tag="B061_RS0104095"
     CDS             5403..6212
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676667.1"
                     /locus_tag="B061_RS0104095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_026241155.1"
                     /transl_table=11
                     /translation="MRFRQQALSKLQSPEDIDLPVRYARPRGGLVLVVTLAVMVGAAFW
                     AVTGTVSSTLSASGVLTHAQGSYVLQSPVAGQVTDVLAEEGERVAADTPLVKVRTSQGS
                     TVVRTLAAGRLTTLAATIGSVITPGADVAAVERVAKAGDPLMAMLYVPAGSGATVPVGA
                     SVDLTVQSVPTQQYGVLRGKVESVTRTAQTRQRISGFLGSSQLGEQFSRQGPPVAVLVR
                     LATDPDGGESGFAWSSSQGPPYRLDSMTLAGGSIELAEQHPIEWLLP"
     misc_feature    complement(5414..5431)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.48"
                     /tool="antismash"
     misc_feature    complement(5426..5438)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.61"
                     /tool="antismash"
     gene            6197..8473
                     /locus_tag="B061_RS0104100"
     CDS             6197..8473
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007500812.1"
                     /locus_tag="B061_RS0104100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_267880919.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-35, bitscore:
                     118.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAASMTAPAPSTHKLHRARAARKARNPRNPRNPRKAAPAKRPKSV
                     RTPTVLQMEAVECGAAALAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAARSYGL
                     QATGMQMESAALAGVRAPAILFWEFNHYVVYDGPDRRLGRRGVYVNDPDKGRRFVPDED
                     FDTSFTGVVLVLEPTGDFRRGGRKPGVLRAIPDRLRSTRPTLLAALLASLLLVAVGAAV
                     PALSRTYIDMFLIGGQTSLLGPLFASMAAMVALTATLTGLQQANLLRGRIISSTLGSAR
                     FLRHLLRLPVTFFAQRSPADLVQRLQSNDTVAETLARDLAAAGVDGVVVILYALLMWTY
                     DPQLTIVGVLIALLNIVAMRVVIRLRAAHTQKLRADTARLTNTSYTGLQLIETMKATGG
                     EDGYFRRWAGQHATTLEEQQRLGVPTAVLSVVAPTLATLNSALILWIGGLRAVEGYISI
                     GLLVAFQALVARFSAPITRLNGVAGRIQDFAADVARLRDVESFPVDPLYSRDEPEPGTR
                     RLTGHVTLEDLAFGYSPLDKPLLTGFSLTVGPGRQIALVGGSGSGKSTVSRLISGLYTP
                     WEGTIRIDGQRLEDIPRGALAASVSFVDQDIFLFEGTIRDNVALWDPSITDEAVITALR
                     DAELYEDVIARRPEGIHGRVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDEVTSALDA
                     RTEQAVIANLRRRGCACVVIAHRLSTVRDSDEIVVLDHGTVVERGRHEALVAAGGPYAE
                     LVREH"
ORIGIN
        1 gcggcaacga gtcgtacacg tacaccgcca agctcgaact ggaaggcaag ctcagcggaa
       61 acacgggccc cgtcaagggg acgctgagcg gcaagcagaa ccgcaccggc gccatcaccg
      121 tcacccgcga ccagaagacc gggaagatcg tccgcatcga catgacacag acggtggaga
      181 ccggctcgcc ctcgggcaag gtcgaagtgg gcggcgacaa cggcgagacg ggggccgaca
      241 agcgcggggg caagggcagc gtgtcggacc cctcgggcga gagcggcatc gacgtggtga
      301 cgaactcgat cgtgttcggc aaggagaccg acgcggccac ggaggcgaag cgggcggtgg
      361 ccgagcagtg gctggacggc tccggcgaca acaccgcccc gttcgcgtac ctcttcggcg
      421 accacggcat ggagacgcgc ccggcgggca acgacccttt cgcacagctc atgttcgagg
      481 acggcctgtc cagcacgatg cgctacctcg gggagacgga cgcgcaggag ttcggcttcg
      541 agatctcgct cgggatgagc ctcgggttct cgctctccga cgagcagaag aaggagacgc
      601 tgacggacgc gcggttcctg ggggcgccgc ggggtgacgg ccgcacgtat cttccgtaca
      661 gctactgcgc gaactgacgg ccaccgtacg gtcggtggat ctgaagggcg cggacggtgg
      721 acctgagagg cgatgacgag atgacgagaa ggctccacgc gggcaggcgg gccgcgacgg
      781 ccgccctggg cctgtccctc ctcctggcac tggccggctg tggcgacgat gccgccgacg
      841 ccgacggcaa ggcctcggtc ccggagggct ggtccacgct ggacacggac agcctgagcg
      901 tcggctaccc gaaggaccag ggatacgcgg agcagtcggc ggccgaacgg agcaggagca
      961 acgcggcggt ggccctgaag gtggaggacg gcatccgcac gggcatggtc tccgtccagc
     1021 tgaacttcgc cacaggcgtg agcgacgcgg ccgaggcggc agcggccgct ggagccggaa
     1081 tcggcctggg cgccacccgc aaggccacgg aggacgtccg cctggccggc ccttcgcccg
     1141 cccgggacgc ccgccgcatc gactacgact tcacggccga cggcaaggag gacaccccga
     1201 ccagggggac cccgatgacg ggggtcctgg tagcgggggt ggactcgggg ggcgtgccgt
     1261 tcgtggtgcg gctgaacgcg gtgagggagg cggtggacgg ggaggacttg gacgcgttcg
     1321 tggggtccat cacggtgaag tagggggtgg gtggcgttca ggcggggcgg ggggcattgg
     1381 cacattggca caccactgcc gcccgcgggc ctgttcgctg ggcggcaccc ggacgaacgc
     1441 accatcaccg tcgcctgtgc ctgcctcgta gaccccaccc gttccatccg gactacccgc
     1501 tgcacccgcc ccagccgcca cgccggatgc cggcccctgc ggccgcctcc acacgaacgc
     1561 cgcaatgccc gaccgggcca tctgatcact tcgcccgtca ggcatccacc cctgcgagga
     1621 tgggcgggtg gcaagagtgg cgaaaggtca acaacttgca aagtccctct aacgccgcag
     1681 gtcaggaaga gtggtccccg tgcaggcggg ggtgttccgc gcctccaggt cgacaccgcc
     1741 gccgcgaata agtggtcccc gcgcaggcgg gggtgttccg ccgcacaccc accgccaccg
     1801 aacgcaaagg acgtggtccc cgcgcaggcg ggggtgttcc gggcggtaca gacgagcccc
     1861 gaatcccgaa gcggtggtcc ccgcgcaggc gggggtgttc cgtacggcta cggcgcccag
     1921 ggcgcccaca tcacgtggtc cccgcgcagg cgggggtgtt ccggcgtggc gggtcgacct
     1981 gctgaacgag gtggggtggt ccccgcgcag gcgggggtgt tccgatgagg ccgccgccca
     2041 ccgccaggac ccagatgtgg tccccgcgca ggcgggggtg ttccgcgctc agggccaacc
     2101 agagacaccg cacagccgtg gtccccgcgc aggcgggggt gttccgcggg agaaccgttc
     2161 gacgatggtc aggccgctgt ggtccccgcg caggcggggg tgttccgctt cgcatcacgc
     2221 tcaacccggc gtcgcaggtg tggtccccgc gcaggcgggg gtgttccggt cacgtccggt
     2281 cagaacggcg ggcagggagg gtggtccccg cgcaggcggg ggtgttccgc tcacctggga
     2341 gggggccagc gccttggtct tgtggtcccc gcgcaggcgg gggtgttccg tcttcaacca
     2401 gatgaacgac ttctacgacg cggtggtccc cgcgcaggcg ggggtgttcc gtgcttgtgc
     2461 accgtctcgc cctcgcgcgt gccgtggtcc ccgcgcaggc gggggtgttc cgttgtcgac
     2521 ggccgcgagc gcctgcggca gctcgtggtc cccgcgcagg cgggggtgtt ccgccggcga
     2581 cgccgagcat gaacaggcgg gctccgtggt ccccgcgcag gcgggggtgt tccgctgtcg
     2641 acttcatcgc gcggacgaca catccggtgg tccccgcgca ggcgggggtg ttccgccgtg
     2701 acccgggtgg gttagggggg tcggcgggtg gtccccgcgc aggcgggagt gttccgatgt
     2761 ccttagaatc caagggtcaa gggggtgggt ggtccccgcg caggcggggg tgttccgacc
     2821 tcgcgcagct gatcgcccgc ctcaaagacg tggtccccgc gcaggcgggg gtgttccgaa
     2881 cgccggcggc gccgacggca cggccaagaa gtggtccccg cgcaggcggg ggtgttccgg
     2941 cgatcgagga gcgagcccag cggtcgcaca agtggtcccc gcgcaggcgg gggtgttccg
     3001 ctggacgccg tccgcgagta cctggagcag cggtggtccc cgcgcaggcg ggggtgttcc
     3061 ggtggacccg gccttcaagc cgagcgacaa gcagtggtcc ccgcgcaggc gggggtgttc
     3121 cggagcgggc gtcccaccac cacatcgacg tcgcgtggtc cccgcgcagg cgggggtgtt
     3181 ccggcctgtc cgcgaaggct tacctgatgt acgccgtggt ccccgcgcag gcgggggtgt
     3241 tccggcagcc gcgaaattcg gcaccttctc cggggcgtgg ccccgcgcag gcgggggtgt
     3301 tccggagcca ccggcgacag tgtgggggag gggtagtggt ccccgcgcag gcgggggtgt
     3361 tccgtccacg cccggtccgt ccggcccgaa ggttccgtgg tccccgcgca ggcgggggtg
     3421 ttccgtccac cggcatctgg cccgactgga ccgggccgtg gtccccgcgc aggcgggggt
     3481 gttccgccgg atgcgcccct cacggtcgcc gcggccttgt ggtccccgcg caggcggggg
     3541 tgttccgacg cacaagggcg acaccatcct caacctcgcg tggtccccgc gcaggcgggg
     3601 gtgttccgcc ggatgcgccc ctcacggtcg ccgcggcctt gtggtccccg cgcaggcggg
     3661 ggtgttccga cgcacaaggg cgacaccatc ctcaacctcg cgtggtcccc gcgcaggcgg
     3721 gggtgttccg ccgccgagac atggatcgcg gggcgtatcg acgtggtccc cgcgcaggcg
     3781 ggggtgttcc gacgatcacg gcggccgtcg ccgcgatgcg gttgtggtcc ccgcgcaggc
     3841 gggggtcttc ctccgcgact gacggaggca acggcttgct cgcggtggtc cccgcgcagg
     3901 cgggggtgtt ccggctcttg ctgctgcgct ggaactcacc cttgggtggt ccccgcgcag
     3961 gcgggggtgt tccgatccgt tggcatcccg gagcttggag agcagcgtgg tccccgcgca
     4021 ggtgggggtg ttccggtcgt cgcgatctcg gtagccgggg ttctcgtgtg gtccccgcgc
     4081 aggcgggggt gttccgccgg cagagccatc ttcgccgtcg gctggttcgt ggtccccgcg
     4141 caggcggggg tgttccgacg tacaccaccg accccgatgc cgagccgcag tggtccccgc
     4201 gcaggcgggg gtgttccgcc ggcggtcgcg gctccgatgc ggaggttgcg gtggtccccg
     4261 cgcgcaggcg ggggtgttcc gtcgggccct ccgctggaac cgcttggcag ttcgttccct
     4321 gcgcaggcgg tcgtgttccg ttgagcccct ggatgagccg gatggtaaac cgggtagtac
     4381 cgggcgaacc tggggccgtg cggcctgggg tgaccggcgc ccgaaagggc cgtaccggat
     4441 aaagccgcat caggccggac cgtacggcct gtaaatgtgg cgcgaaccgg tccagcgccg
     4501 agcactgaac acgtatagcg acaaaatgca cactcgcggg atacacccgt cggtaattta
     4561 actcctcttt catagatctc caccgcggac gcgcagcatt cacattccgt cccgctttcc
     4621 gtttccgttc cgctcgaggt cggggcgcca gggaggcgag ggcggttccc tcggtgccgg
     4681 tcgccgcgcg gattggtctc tcacctggtg gatcgtgtga agagacgtca ccttccctat
     4741 gactgcctct tcacctcagg ccaattcgct atcgccgtaa gccctcttcc gcttcggtgc
     4801 gattggccgc atggatggag tgcgtgcttt gatccatcgc actgcggcag tcggcccggc
     4861 catttcagtc catggagccg cgcaagggat tcatgaatcc tgctcagctt tctgggaccg
     4921 tcaccgttcg accggtcaaa cgaatgttcc gagccgacca ccgcacgccc tcgaaatatc
     4981 catgtgaagg gaagctcctc atgaactcca ccccccaggt cccgacccag gtcatctccg
     5041 acgccgacct ggacaacgtg tccggtggca tcctcaacgg ttccctgaac aacaacgccc
     5101 tcggcctcgc cgccgacgcc ccggtctcgc tgtccggtgc gctccaggcc gccaccggca
     5161 ccgttgacgc cgccaccggc ctcaacctgc agggtgtctc gggtctcgtc tcgggtctct
     5221 gatccggccg tgtgccggtg aacggcacac ttcacggtag tgatgccggg ccgccggttc
     5281 tccccatacc ggtgcgccgc cccgtgtgcc ccggatccca ccggatccgg ggcacacggg
     5341 gtactccgtt ctcccgtacg caggtgaacg acggcaaacg atggcagtcg aggaagagtg
     5401 tcgtgcgctt tcgtcaacag gccctttcca aactgcagtc cccggaagac atcgatctgc
     5461 ccgtgcgcta cgcgcgcccg cgcggcgggc tcgtcctcgt cgtgacgctc gcggtcatgg
     5521 tcggcgcggc cttctgggcg gtgaccggca ccgtgtcctc cacgctcagc gcgtcgggcg
     5581 tcctcaccca cgcgcagggc agttacgtcc tccagagccc cgttgccggg caggtcaccg
     5641 acgtcctcgc cgaggagggc gagcgggtgg ccgccgacac ccccctcgtg aaggtccgta
     5701 cgtcgcaggg cagcacggtc gtccgcaccc tggccgccgg ccggctcacc acgctcgccg
     5761 ccaccatcgg ctccgtcatc acccccggcg ccgatgtggc ggccgtggaa cgcgtcgcca
     5821 aggccggcga cccgctcatg gccatgctgt acgtccccgc cgggagcggc gcgaccgtac
     5881 ccgtcggcgc ctccgtcgac ctcaccgtgc agtccgttcc cacccagcag tacggcgtgc
     5941 tccgcggcaa ggtcgagtcc gtcacccgga ccgcccagac gcgccaacgg atctccggct
     6001 tcctgggcag cagccaactg ggcgagcagt tctcccggca ggggccaccg gtcgccgtgc
     6061 tcgtgcggct cgccaccgat ccggacggcg gcgagtccgg cttcgcgtgg tcgtcctcgc
     6121 agggcccgcc ctaccggctg gactccatga cgctggccgg cggctccatc gaactcgccg
     6181 agcagcatcc gatcgaatgg ctgcttccat gaccgctccc gccccctcca cccacaagct
     6241 tcacagggcc cgggcggccc gcaaagcccg taatccccgc aacccccgca acccccgaaa
     6301 agccgcacct gccaagcggc ccaagagcgt ccgtaccccg accgtcctcc agatggaggc
     6361 cgtcgagtgc ggtgccgccg ccctcgcgat ggtgctcgcg caccacgggc ggcacgtgcc
     6421 gctggaggag ctgcggatcg cctgcggggt gtcgcgcgac ggctcgcgcg ccagcaacgt
     6481 actgaaggcg gcgcgcagtt atggtctcca agccaccggc atgcagatgg agtccgccgc
     6541 cctcgccggg gtgcgggcgc ccgccatcct gttctgggag ttcaaccact acgtcgtgta
     6601 cgacggcccc gaccgtcgcc tcggccggcg cggcgtgtac gtcaacgacc ccgacaaggg
     6661 ccgccgcttc gtgcccgacg aggacttcga caccagcttc accggcgtcg tcctcgtcct
     6721 ggagccgacc ggcgacttcc gccggggcgg ccggaagccc ggggtcctgc gagccatccc
     6781 cgaccggctg cgctccaccc ggcccaccct gctcgccgcg ctgctcgcca gcctgctgct
     6841 ggtggccgtc ggcgcggcgg tgcccgcgct gagccgtacg tacatcgata tgtttctcat
     6901 tgggggtcag acctcgctgc tggggccgct gttcgcctcg atggcggcca tggtcgcgct
     6961 gaccgccacc ctcaccggtc tgcaacaggc caatctgctg cgcggccgga tcatctcctc
     7021 caccctcggc agcgcccgct tcctgcgcca tctgctgcgg ctgccggtca ccttcttcgc
     7081 ccagcgcagc cccgccgacc tcgtgcagcg gctccagtcc aacgacacgg tcgccgagac
     7141 gctggcccgg gacctcgcgg cggccggtgt ggacggcgtc gtcgtgatcc tgtacgcgct
     7201 actgatgtgg acgtacgacc cccagctcac catcgtcggc gtgctcatcg ccctcctcaa
     7261 catcgtcgcc atgcgggtcg tgatccggct ccgcgccgcg cacacccaga agctgcgcgc
     7321 cgacaccgcc cggctgacca acacctcgta caccgggctc cagctcatcg agacgatgaa
     7381 ggccaccggc ggcgaggacg gctacttccg gcgctgggcg ggacagcacg ccaccaccct
     7441 ggaggagcag cagcggctcg gcgtgcccac cgcggtgctc tccgtcgtcg caccgacgct
     7501 cgccacgctc aacagcgcgc tgatcctctg gatcgggggc ctgcgcgcgg tggaggggta
     7561 catctcgatc gggctgctgg tcgccttcca agccctggtg gcgcggttca gcgcgccgat
     7621 cacccggctg aacggagtgg cgggccggat ccaggacttc gcggcggacg tggcgcgcct
     7681 gcgggacgtc gagagctttc ccgtcgaccc gctgtactcg cgcgatgagc cggagccagg
     7741 cacccgccgg ctgaccgggc acgtcaccct ggaggacctc gccttcggct acagcccgct
     7801 cgacaagccg ctgctcaccg gcttctcgct gaccgtcggg ccgggccggc agatcgccct
     7861 cgtcggcggg tccggcagcg gcaagtcgac cgtctcccgg ctgatctcgg gcctctacac
     7921 cccgtgggag gggaccatcc gcatcgacgg gcaacggctg gaggacatcc cccggggtgc
     7981 gctggcggcc tcggtctcct tcgtcgacca ggacatcttc ctcttcgagg ggacgatccg
     8041 ggacaacgtg gcgctgtggg acccctcgat caccgacgag gccgtgatca cggcgctgcg
     8101 ggacgcggaa ctgtacgagg acgtgatcgc ccggcgcccg gagggcatcc acgggcgggt
     8161 cgagcaggac ggccgcaact tctccggcgg ccagcgccag cggctggaga tcgcccgcgc
     8221 gctggtgcgg cggccgagtg tcctcgtact cgacgaggtg accagcgccc tggacgcccg
     8281 gaccgagcag gccgtcatcg ccaatctgcg gcgccgcggg tgcgcgtgcg tggtgatcgc
     8341 gcaccggctg agcacggtgc gggacagcga cgagatcgtg gtcctcgacc acgggacggt
     8401 cgtggagcgg ggccggcacg aggcgctggt cgcggccggc ggaccgtacg ccgaactggt
     8461 cagggagcac tgagcgccgc catgacgtac gttccgcctc cgggcgcctc tgtggtgcct
     8521 gcctccgact ctgtgatcca tgcgctgggc gggctcggct cgccggtcga gggcgcgggg
     8581 ctggggagcg ttccgctgga ggggccgcag gtgttgtggc tggtggtgga gggggcgctc
     8641 gacctgttcg cggtcgacgc gcaccagcag gggcactggc actttctggg gcgcctcgac
     8701 ccgggcgggc tgctgctcgg gccggtggag gggccgcagc acacgctgct cggccggccc
     8761 tcccaggact gccggctgcg gcggatcgcg ttgcgggagc tgtggcggcc ggagccgtac
     8821 ggggatgggt acggtgatca gacgtacggc gtgcgatacg gaccctcgca gggtgacccg
     8881 tacgcccccg ccgggccgac gccgctggag cacgcggtct ccctcggggc cgggcgcggc
     8941 accggtgtcc tctccgccgc gcccctggac ggccggcccg ccgccgcctc cggcgcggcc
     9001 gtgtccgacg acgatgtgct gtggctgccg gttccgccgg ggagcgtgca gtacggcgcg
     9061 tcctacggtg cggaggcctc cgcggacctg ctgatggacg cggagctgtg gcagcggatg
     9121 gtggaccagc agtaccggct gctcaccgcc gtcgaccgct ggatcgagca ggtggagcgc
     9181 gcccacgagg accgtacggc cgccggacta cgcgcgggcg aggcggtccg ggccagcgcc
     9241 gaccaggcgt tgctggcctc gatcgggcgg ccgggcggcg gcggtgcggc ggctgccggt
     9301 ggcgggccgg gggccgggga cgatgccacg tacgccgtgt gccggcgggt cgcggaggcg
     9361 gccgggatca ccctgaccga gccgccgaag ggggctgccg gggagagcga ccgcatcggt
     9421 gcggtggagc gcatcgcgct cggctcccgg gtgcgcaccc gtgccgtacg gctcgacggg
     9481 gcctggtggc gcaacgacgc agggccgctg gtcgggcagc gcgccaagtc cggggcgccg
     9541 gtggcgctgc tgtggcggcg gggccggtac gaggcggtca acccggtcac cggcagccgg
     9601 atccgggtcg acgaggacaa cgcggaggac ttcgcggagc gggcggtgat gttctaccgg
     9661 ccgttgcccg agaagccgat gcgcacggcc cggctactgc tgttcagcct ccggggcacc
     9721 cggagcgacc tgcgcaacct cctcatcagc gggctggtga cggtcgggct cggcgctctg
     9781 gtgccgatcg cgacgggtca ggttctcggt acgttcgtgc cccgggcgca gacggatctg
     9841 atcgcgcagg tgtgcctggt cctgatgatc acgagcgtcg tcggcgccac gttcatgctg
     9901 ctgcagagcc tcacgctgct gcggatggag ggccggatcg agagcacgtt gcaaccggcc
     9961 atctgggacc gcctgttgag actgccgacg aagttcttca ccgagcgttc gacgggtgaa
    10021 ctggcgagcg ccgcgatggg gatcagcgtg atccggcggg tgctggcggg agttgggccg
    10081 gtcgccgtgc aggcggggac ggtcggggcg gtcaacctcg ggctgctgat cttccacagc
    10141 gtgccgctgg cgctggcggc gatcggcatg ctcgccgtga tcgcggccgt gttcctggcg
    10201 ctgggcctgt ggcaactgcg ct
//
