LOCUS       NZ_KB890946            11491 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. CcalMP-8W B053DRAFT_scaffold_5.6, whole genome
            shotgun sequence.
ACCESSION   NZ_KB890946
VERSION     NZ_KB890946.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. CcalMP-8W
  ORGANISM  Streptomyces sp. CcalMP-8W
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 464790)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   2  (bases 1 to 464790)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. CcalMP-8W
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:14:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,603
            CDSs (total)                      :: 6,529
            Genes (coding)                    :: 6,424
            CDSs (with protein)               :: 6,424
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 1 (5S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 83 of 105
            Pseudo Genes (internal stop)      :: 5 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:04:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 286178
            Orig. end                         :: 297669
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB890946.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405361
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11491
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11491
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11491
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6491](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6491
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(72..749)
                     /gene="dhaL"
                     /locus_tag="B053_RS0126755"
     CDS             complement(72..749)
                     /GO_function="GO:0004371 - glycerone kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="dhaL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512449.1"
                     /locus_tag="B053_RS0126755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="WP_018491667.1"
                     /transl_table=11
                     /translation="MNAGAGSAGFTSGWMRRFAEAAHDGQPLLTALDQQVGDGDFGVNL
                     TAGVDAARPRLDRLPDRGADPSAPLLATAEAFLDEVGGTSGPLFGLLLHSLAAAIAEDG
                     LTTAALADGFASGLAAIQRVGGARPGDKTLVDALAPATDALRSTDPSAPAADALAAAAA
                     SAWDGVAATARLRARMGRSSYLGERTEGIPDPGAVGVALLLSCAQAGKPDLGAHLDAVR
                     AAG"
     gene            complement(797..1789)
                     /locus_tag="B053_RS0126760"
     CDS             complement(797..1789)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947698.1"
                     /locus_tag="B053_RS0126760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="WP_018491668.1"
                     /transl_table=11
                     /translation="MSAYFETATGDLASEALHSFADVHAELVSADSRFGFLTARDPAET
                     RTVGLLSGGGSGHEPLHGGYVGRGMLDAAVPGRVFASPHNRQVYEASLAVARPGGVLHL
                     VKNYTGDRINFGIAAERLAHKGIPCARVLIDDDLASDAEGIAAGRRGTGGTVLVEKILG
                     GAADRGMGLAELQDLGERLVAGCRSLAVATAAHTSPATGGPAFTLDEGRLEYGVGIHGE
                     RAGSTVSRRPLAELVERMTTELLDALPADVPVISLVNGLGATTLLELYAAHGQLARVLR
                     GAGVPLARSLVGNYITSLDMHGFSITLMAADPPALGLYDAPVHTPALRW"
     gene            complement(1996..2184)
                     /locus_tag="B053_RS0126765"
     CDS             complement(1996..2184)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="B053_RS0126765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_026238161.1"
                     /transl_table=11
                     /translation="MDDDASYCTPPAVTPPTGADTAEAFAECVLCREPTEYPESAKGAT
                     LCPVCAWQEAGRTACSG"
     gene            complement(2211..2885)
                     /locus_tag="B053_RS0126770"
     CDS             complement(2211..2885)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="B053_RS0126770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AIM24 family protein"
                     /protein_id="WP_018491670.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHLAQPATAPGMTLQNSKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGLLKRAVTGEGLPLMAVRGQGEAWFAHEAANCFIVEMDQGDQLT
                     INGRNVLCFDATLSYEIRTVKGAGMVGGGLFNSLFSGYGKIGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQHLTTTLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIIRPSEARPERT
                     AN"
     gene            2957..3784
                     /locus_tag="B053_RS0126775"
     CDS             2957..3784
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514518.1"
                     /locus_tag="B053_RS0126775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_018491671.1"
                     /transl_table=11
                     /translation="MSSDDTSPSPSGDIPPSPLGEGPAGSAGPTGSAGPTGPTGSARPA
                     DRDAAPQYVLPLVLHLEKTDPPARTDALRTAARAVLTILADERSTGEGEWARAMRDWED
                     ARIRKVVRRARGAEWRKAAALPGITVTGGSAEVRVYPPIPLDGWPKELAKLQVSGTDLD
                     DPEPPAAPGPEGPVLWLNPELEMSAGKTMAQVGHGAQLAWWELSDTDRKAWREEGFPLS
                     VATATPDRWRELVTSGLPVVRDAGFTEIAPGPTVVAEGGERLCPLPGGRPGRR"
     misc_feature    complement(4050..4065)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     misc_feature    4061..4076
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            4090..4836
                     /locus_tag="B053_RS0126780"
     CDS             4090..4836
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="B053_RS0126780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_018491672.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATVGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHADLDRRAR
                     DVAAKLGVELPNQPTEQQQAWLRELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGFVDFDALAREAAGGSTASPSGPPAPREGQVPL
                     APVPVTPSGDQSFTSRPVPPTMPPMP"
     gene            5001..6491
                     /locus_tag="B053_RS0126785"
     CDS             5001..6491
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059402.1"
                     /locus_tag="B053_RS0126785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_018491673.1"
                     /sec_met_domain="DUF692 (E-value: 1.6e-93, bitscore: 307.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGIGIGWRPEIAAEVEALTGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVSLGLGGADRPDPERLSGLAARAELLGTPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALDVLCENVRVAQDALPVPLALENIAALVSWPGEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNRVNRGEDPAKALDELPVEAIAYVHVAGGVEKDGVWHDTHA
                     HPVTAPVLDVLAELRSRVDPPGVLLERDDAFPPGAELAAELATIRGTLRAAAAAAPAPR
                     TGAAVAAATVGPTAPAATVAPVAPVAPTGAAASAGAMAAVAPAPSTPAAAAAPAAAAAP
                     LTSSAPSADGRAAEPLRTPRPGARDRTAVAQTALLSALVAGTPAPEGFDHRRLRVQSRA
                     LAAKRADVVAKVAPELPEILGDGYRAAFLPYAVSRPMNGGYRRDAMEFVERILVSGGLP
                     DPVAQRRLTHWWEDRSGLRPPGRATRLVRAARAVLAGK"
     gene            6500..7531
                     /locus_tag="B053_RS0126790"
     CDS             6500..7531
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514514.1"
                     /locus_tag="B053_RS0126790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_026238163.1"
                     /transl_table=11
                     /translation="MNTFALLLTLAVVLSTALLIAKAVASRSGRRTPVGSPYPVRGLYE
                     AAFLNGGPARVADTALTTLYGDGRLLIGGPGIVSVQREQAHDPVEHAVFQVLATAPNGA
                     LHAVREAVMRHPAVQGIGDGLAERGLLVPPAASRPVRRWGLVQGVSCLVGFPLAIAATV
                     IQYVTHDSPFDIPFPFVLKVLPAILLGGISGLVVAAAASGRLTRAGRRAAHEFRTAHAY
                     QATPPYLVATQGLRALPDPVLQAQLIAAAGANGPSGRSRHRYRTAGVVGGTAAAGGFAA
                     TTVWCADAGPGHTSCGGSSGDGSGSGCGGGSGCGGSGSSCGGGSSCGGGSSCGGGGGCG
                     GGS"
     gene            7733..9301
                     /locus_tag="B053_RS0126795"
     CDS             7733..9301
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-289). E-value:
                     4.5e-16. Score: 51.1. Matches aSDomain:
                     nrpspksdomains_B053_RS0126795_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="B053_RS0126795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_018491675.1"
                     /sec_met_domain="PF00561 (E-value: 1e-21, bitscore: 72.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVPLYATIGSLVLSALAGTAPAAASERNGGGSAEERGTAVATA
                     RAAAAGISFGACPEEEMFPDSLECGTVRVPLDYARPDGPAIPLTVSRTRATGEPAQRQG
                     SFVYNPGGPGASGITFPLLGEMSEWKEVAEAYDLVGYAPRGVGTSAPLSCQDPAVFAQS
                     PTDSPAHPTTAYKEKRIAQARAYARGCEESAGEALRHYTSLNNARDLEVLRAALGDERL
                     TFMGASYGTYIGSLYATLFPARVRRMVFDSAVDPDPEKIWYRSNLDQSLAFESRWEDFR
                     RWVARHDDVYGLGASPEAVQRHYDDTRTALAAEPAGGTVGPGQLHAGFLQAAYYDDSWA
                     MRATALSEYVRGNPEPLVTQLSPRAVTARDDENERAVYTAVECNDAPWPEEWEVWDRDH
                     TDLARIAPFQTWHNAFTNLPCAFWPAPRQRPLDVSTGWGELPPVLILAAERDAATPYQG
                     ALELQRRLRGAVLVTERGAGTHGVGGFGNACVDEHMRRYLLTGETPAGDAECAPRAEPN
                     PVSLD"
     aSDomain        8048..8599
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B053_RS0126795_Abhydrolase_1.1"
                     /evalue="4.50E-16"
                     /label="B053_RS0126795_Abhydrolase_1.1"
                     /locus_tag="B053_RS0126795"
                     /protein_end="289"
                     /protein_start="105"
                     /score="51.1"
                     /tool="antismash"
                     /translation="FVYNPGGPGASGITFPLLGEMSEWKEVAEAYDLVGYAPRGVGTSA
                     PLSCQDPAVFAQSPTDSPAHPTTAYKEKRIAQARAYARGCEESAGEALRHYTSLNNARD
                     LEVLRAALGDERLTFMGASYGTYIGSLYATLFPARVRRMVFDSAVDPDPEKIWYRSNLD
                     QSLAFESRWEDFRRWVARHDD"
     gene            complement(9462..10190)
                     /gene="hemQ"
                     /locus_tag="B053_RS0126800"
     CDS             complement(9462..10190)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="B053_RS0126800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_026238164.1"
                     /transl_table=11
                     /translation="MSAPETVTPSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRT
                     GYADEVQELFDQLAAKDVTVRGTYDLSGLRADADLMIWWHAETADQLQDAYNLFRRTEL
                     GRALEPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EIPFYTGRRKDIADLVAGLA"
     misc_feature    11460..11474
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 cccccgagtt gctggaactg gactgggtgc gccccgggta gccgcggggc ggcggcgtcg
       61 cggggagacc gtcagccggc ggcgcgtacg gcgtccaggt gcgcgccgag gtcgggcttc
      121 ccggcctggg cgcaggagag caggagggcg acaccgacgg cacccgggtc gggaatcccc
      181 tcggtgcgct cgcccagata gctggaacgt cccatgcggg cccggagcct cgcggtggcg
      241 gcaacgccgt cccacgcgct cgcggccgcg gccgcgagcg cgtcagcggc gggggccgac
      301 gggtcggtcg agcggagggc gtcggtggcc ggtgccaggg cgtcgacgag ggtcttgtcc
      361 ccgggcctgg caccgccgac acgctggatc gcggcgaggc cggaggcgaa gccgtcggcg
      421 agggccgcgg tggtgagccc gtcctcggcg atcgccgcgg ccagggaatg cagcagcagt
      481 ccgaagaggg ggccgctcgt accgccgacc tcgtcgagaa acgcctcggc cgtggccagg
      541 agcggcgccg aggggtcggc gccccggtcc gggagccggt ccaggcgcgg ccgggcggca
      601 tccactccgg cggtcaggtt gaccccgaag tccccgtcgc cgacctgctg gtcaagagcg
      661 gtgagcaggg gctgaccgtc gtgggccgcc tccgcgaacc ggcgcatcca gccggaggtg
      721 aagccggcac tgccggcacc tgcgttcatg agagatcctc ccgggctgtc ggctgcggtg
      781 gcaggggacc gttccctcac cagcgcaggg ccggagtgtg gacaggagcg tcgtacaggc
      841 ccagggccgg cgggtcggcg gccatcaggg tgatggagaa gccgtgcatg tccagggagg
      901 tgatgtagtt gcccaccagg ctgcgggcga gtgggactcc ggcgccccgc agcacgcggg
      961 cgagctgccc gtgcgccgcg tacagctcca gcagggtggt ggcaccgagg ccgttgacca
     1021 gcgatatcac cgggacgtcg gcaggaagcg cgtccagcag ttccgtcgtc atgcgttcga
     1081 ccagctcggc cagcgggcgg cgggacacgg tcgagccggc ccgctccccg tgaatgccca
     1141 ccccgtactc cagccgcccc tcgtccagcg tgaaggcggg cccgccggtg gcgggggagg
     1201 tgtgcgcggc ggtggcgacg gcgagtgaac ggcagcccgc cacgagccgc tcgcccaggt
     1261 cctggagttc ggcgagcccc atgcctcggt ccgccgcacc cccgaggatc ttctccacca
     1321 ggacggtgcc gcccgtcccg cgccgtccgg cggcgatccc ctccgcgtcg gaggcgaggt
     1381 cgtcgtcgat gaggacgcgg gcgcagggta tgcccttgtg ggccaggcgc tcggcggcga
     1441 ttccgaagtt gatgcggtcg ccggtgtagt tcttcaccag gtggagaacc cctcccgggc
     1501 gggcgaccgc gagagacgcc tcgtacacct gccggttgtg cggggaggcg aacacccgcc
     1561 cggggacagc ggcgtcgagc atgccccgtc cgacgtaccc gccgtgcagc ggctcgtgcc
     1621 ccgatccacc gccggagagc aggccgaccg tacgcgtttc ggccgggtcg cgtgccgtga
     1681 ggaagccgaa ccgggagtcg gctgacacca gttccgcgtg gacgtcggcg aaggagtgca
     1741 gcgcttcgga ggcgaggtca ccggttgcgg tctcgaagta cgcggacatc tcactcctcg
     1801 gatatccagg cgcaccagcg ggccgaagga ccgcggggcg gacacggtcc catcaggctg
     1861 cgatgccggc cggtgccccg caagtccgag gagagggcgc tctcacgttc gagtacgccc
     1921 tcggtgcgga ccagggccgc ccgccggtcc gccaccggac ggtccggacg gaggaccggc
     1981 ccgggacggc agaacctagc cggagcaggc cgtgcgtccc gcctcctgcc aggcgcagac
     2041 cgggcagagg gtggcgccct tggcggactc ggggtactcg gtcggctcac ggcagagcac
     2101 gcactccgcg aacgcctcgg ccgtgtcggc gcctgtcggc ggggtgactg cgggcggtgt
     2161 gcagtacgag gcgtcgtcgt ccatgcccac gagcgtacgc aaccccgctg tcagttggcc
     2221 gtccgctccg gcctggcctc gctggggcgg atgatgacga agccctcgcc ctggagcatc
     2281 agctggacgg cctcgcccga gccgccgcgg accatcgagc cgacgctctg ggaacggtgc
     2341 agggtggtgg tgaggtgctg gctccagccg acgaccgcgt ccgtgtcgac gtacaccggc
     2401 tgggccgccg tcaccgggat cacgatcggg gtgccctcgc acatcaggcc gatcttgccg
     2461 tagccggaga agaggctgtt gaagaggccg ccgccgacca tcccggcgcc cttcacggtc
     2521 ctgatctcgt acgagagcgt ggcgtcgaaa cagaggacgt tgcggccgtt gatcgtgagc
     2581 tggtcgccct ggtccatctc cacgatgaaa cagttggcgg cctcgtgcgc gaaccacgcc
     2641 tctccctgac cgcgtacggc catgagcgga aggccctcgc cggtgaccgc gcgcttgagc
     2701 aggccaccga tcccctggcc cttgcgctcg aactggaggt tgccgcggaa ggcgatcatc
     2761 gaaccctgac gggcgtgcat ctccccgttg acggcgtact tgatcgactt ggagttctgg
     2821 agggtcatgc cgggggcggt ggccggctgg gccaggtgtt cgctggaaaa cagatcgctc
     2881 ttcatgtccg gcatcctcac ccggaccggc cgattaagcc aaggaggtga cgggggccgc
     2941 ctggcagact gggacggtga gcagcgacga cacctccccg agcccctccg gcgacatccc
     3001 cccgagtccc ctcggcgagg gccctgcggg ttctgcgggc cccaccggtt ccgccggtcc
     3061 cacgggtccc accggttccg cccgtcccgc cgaccgggac gcggccccgc agtacgtgct
     3121 cccgctcgtg ctgcacctgg agaagacgga cccgcccgcc cggacggacg ccctgcgcac
     3181 cgccgcccgc gcggtgctca cgatcctggc cgacgagcgg tccaccggcg agggcgagtg
     3241 ggcgcgggcc atgcgggact gggaggacgc ccggatccgg aaggtggtgc gccgggcgcg
     3301 cggcgcggag tggcgcaagg cggccgcgct gcccgggatc acggtcacgg gcgggagcgc
     3361 ggaggtacgg gtgtacccgc cgatcccgct cgacggctgg cccaaggagc tggccaagct
     3421 ccaggtctcg ggcaccgacc tggacgaccc ggaaccgccc gccgcccccg gcccggaggg
     3481 cccggtgctc tggctcaacc cggagctgga gatgtccgcg ggcaagacca tggcccaggt
     3541 cggccatggc gcccagctcg cctggtggga gctgtccgac accgaccgca aggcctggcg
     3601 cgaggagggc ttcccgctct ccgtggccac cgcgaccccg gaccgctggc gcgagctggt
     3661 cacgagcggc ctgccggtgg tccgcgacgc ggggttcacc gagatcgcgc cggggccgac
     3721 ggtcgtggcg gagggcgggg agcgtctctg cccgctgccg gggggacggc ccggccgccg
     3781 ctgaccacgc ggccgccgag ccgcttcccg cccggctggg cccgcgccga cccgtggccg
     3841 accgggcccg ccgcgacagc ccggggccga ccggaccgac cggaccgacc cagcccgaaa
     3901 tcggtccggt cgcggttgag cattcccgtg cccgaccacg tatctcccga taccgcccca
     3961 gcggcgctac cgccaagtag cctgcaccgc acgccggttg gcggccggcg gtcggtcggt
     4021 gatcggccgg tggccggtgg ccgtccggcg tgacggtgct gtcacgtcct cgtcccggga
     4081 gacaccttgt tgcgacgcat caacggaacg gccctcatca tcgcggccct ggtcgccacg
     4141 gtcggcgcgc tcgccttccc cgtctggtcc tacgcggacc gctcgggcac cggcgaggcg
     4201 aatctcaacg cctccagcgt cgccacccag tggggcccgc tctccgcgac cgaccgggac
     4261 ttcctggtca aggtgcggct cgccggactg tgggagctgc ccgccgggca gcaggcgatc
     4321 gagcgggcgc ccagcgaggc gacgaaggcc gccggggacc atctggtcgt cgggcacgcg
     4381 gacctggacc ggcgggcccg ggacgtggcc gccaagctcg gggtggagct gcccaaccag
     4441 ccgaccgagc agcagcaggc gtggctgcgg gagctgacgg cggcgagcgg ggacgagtac
     4501 gagcggaagt tcgccaacat cctgcgggcc gcccacggga aggtcttcgg gctgatcggc
     4561 caggtccggc acaccacccg caacaccctg atccggcagc tcgccagcga cgccaaccag
     4621 accgtgctgg accacatcac catgctggag gccaccggtt tcgtcgactt cgacgccctg
     4681 gcccgcgagg ccgcgggcgg gtccaccgcc agcccctcgg gcccgcccgc gccccgggag
     4741 gggcaggtgc cgctcgcccc ggtgccggtg acgccgtccg gcgaccagtc gttcacgtcc
     4801 cgcccggtgc cgccgaccat gcccccgatg ccgtgacacg gactgcgggg gccgagggcc
     4861 gcgccggggc cgtggtgccg atgccgtgac gtgacctgcg ggccggaacc tcaaatcctc
     4921 ctctgaacgt ccccttcttc gggttcaccg ccgttccacg gggccatcac ccctggacgg
     4981 agcctagaag ggggagacgg atgaggctgg gcatcgggat cggctggcgg ccggagatcg
     5041 ccgccgaggt ggaggcgctc acagggatcg actgggtcga ggcggtggcg gagaacctct
     5101 gcgccgacca cctccccgac tccctggtcc gcctgcggga gcggggcgtc accgtcgtac
     5161 cgcacggcgt ctcgctcggc ctgggcggtg ccgaccgccc ggaccccgag cggctctcgg
     5221 ggctggcggc ccgtgccgag ctgctcggta cgcccctggt gaccgagcac atcgccttcg
     5281 tacgggccgg ggggccgctg accgcctccc cgcggctgga ggcgggccac ctgctgccgg
     5341 tgccgcgcac ctgggacgcg ctggacgtgc tctgcgagaa cgtacgcgtc gcccaggacg
     5401 cgctgcccgt accgctggcc ctggagaaca tcgcggccct ggtctcctgg cccggcgagg
     5461 agctgaccga ggggcagttc ctggcggagc tggtcgagcg gaccggggtg cggctgctga
     5521 tcgacgtggc caacctgcac accaaccgcg tcaaccgggg cgaggacccg gcgaaggccc
     5581 tggacgagct gccggtggag gccatcgcgt acgtccatgt ggcgggcggg gtcgagaagg
     5641 acggcgtctg gcacgacacg cacgcccacc cggtgaccgc cccggtcctg gacgtcctgg
     5701 ccgagctgcg ctcccgcgtc gaccccccgg gcgtgctgct ggagcgcgac gacgccttcc
     5761 cgccgggcgc ggagctggcg gccgagctgg cgacgatccg cgggacgctg cgcgcggcgg
     5821 cagcggcggc cccggcgccc cggaccggag cggcggtcgc ggcggctacg gtgggcccca
     5881 cagcacccgc ggccacagtg gctccggtgg ccccggtggc cccgaccgga gccgcggcct
     5941 cggcaggagc gatggcagcg gtggccccgg ccccgtcgac acctgcggcc gcggcggccc
     6001 cggcggcagc ggcggccccg ctgacctcgt cggcgccttc ggccgatggg cgtgcggccg
     6061 aaccgctccg caccccgcgc cccggagccc gcgaccgtac cgccgtcgct cagaccgcgc
     6121 tgctctccgc gctcgtcgcc gggaccccgg cccccgaggg gttcgaccac cggcggctgc
     6181 gggtgcagag ccgggcactg gccgccaagc gggccgacgt cgtcgccaag gtggcccccg
     6241 agctgccgga gatcctcggt gacggctacc gcgccgcgtt cctcccctac gccgtgtccc
     6301 gcccgatgaa cggcggttac cggcgcgacg ccatggagtt cgtcgagcgg atcctggtct
     6361 ccggcgggct ccccgacccc gtcgcacagc gtcggctgac gcactggtgg gaggaccgct
     6421 cggggctccg gccgccgggc cgggccaccc gtctcgtccg ggcggcccgt gccgtcctcg
     6481 cagggaagtg accaccgcca tgaacacgtt cgccctgctg ctgaccctcg cggtcgtcct
     6541 ctccaccgcg ctcctcatcg cgaaggccgt cgcctcccgg tcgggccgcc gcacccccgt
     6601 cggctccccc taccccgtcc gcgggctcta cgaggcggcg ttcctcaacg gcggcccggc
     6661 cagggtcgcg gacaccgcgc tcaccaccct gtacggggac gggcgcctgc tcatcggcgg
     6721 ccccgggatc gtctccgtac agcgtgaaca ggcgcacgac ccggtggagc acgccgtgtt
     6781 ccaggtgctg gcgaccgcgc ccaacggcgc cctgcacgcg gtacgggaag cggtcatgcg
     6841 ccatccggcg gtgcagggga tcggggacgg gctggcggag cgcgggctgc tggtgccgcc
     6901 cgccgcgagc cgtcccgtcc ggcgctgggg gctggtccag ggggtgagct gcctcgtcgg
     6961 cttcccgctc gccatcgccg ccacggtcat ccagtacgtg acccacgact cgccgttcga
     7021 cataccgttc ccgttcgtcc tgaaggtgct gcccgcgatc ctgctcggcg ggatcagcgg
     7081 gctcgtcgtg gcggcggccg cgtcgggccg gctcacccgg gcggggcgcc gcgccgccca
     7141 cgagttccgg accgcccacg cctaccaggc caccccgccg tacctcgtcg ccacccaggg
     7201 gctgcgcgcc ctccccgacc cggtgctcca ggcccagctg atcgcggcgg ccggggcgaa
     7261 cggaccgtcc gggcggtcgc gtcaccggta ccggacggcg ggggtcgtcg gcggtacggc
     7321 agcggccggg ggcttcgcgg ccaccacggt gtggtgcgcc gacgcgggcc ccgggcacac
     7381 gagttgcggc ggatcgagcg gcgacgggtc gggttccggg tgtggcggag ggtccgggtg
     7441 cggcggctcc gggtcgagct gcggcggcgg ttccagctgc ggagggggct ccagctgcgg
     7501 aggcgggggc ggctgcggcg gaggttcctg agccgtcaac gcgcgggggg ggcgggccct
     7561 gagccgtcag cccctgaggg cggccctgag ccgtcgtccc gggggcggct cggggtcctc
     7621 accctccagg tggcgcgaac gggttgatcc gcgcaacacg cccgcccggt actggacttc
     7681 accggccgcc acccccacca ttccctctcc gcatccgcag agaggccccg ccgtgagagc
     7741 agtccccctg tacgcgacga tcggctcgct ggtcctgtcg gcgctggccg gcacggcacc
     7801 ggccgccgct tcggagagga acggcggcgg ctccgccgag gaacgcggta cggcggtggc
     7861 gacggcccgc gccgcggcgg cggggatctc cttcggggcg tgcccggagg aggagatgtt
     7921 cccggactcg ctggagtgcg gcaccgtacg ggtccccctg gactacgccc gcccggacgg
     7981 cccggccatc ccgctgaccg tgagccgcac ccgcgccacc ggtgaacccg cccagcggca
     8041 gggctcgttc gtctacaacc cgggcgggcc cggcgcctcc ggcatcacct tcccgctgct
     8101 gggcgagatg tccgagtgga aggaggtggc cgaggcctac gacctggtcg gctacgcccc
     8161 gcgcggcgtc ggcacctccg ccccgctctc ctgccaggac cccgccgtct tcgcgcagag
     8221 ccccaccgac tcccccgccc accccaccac cgcgtacaag gagaagcgga tcgcccaggc
     8281 gcgggcgtac gcgcgagggt gcgaggagag cgcgggcgag gcgctgcgcc actacacctc
     8341 gctcaacaac gcccgtgacc tggaggtgct gcgggccgcc ctcggggacg agcggctgac
     8401 cttcatgggc gcctcgtacg gcacgtacat cggctccctg tacgcgaccc tcttccccgc
     8461 gcgcgtccgc cggatggtct tcgactccgc ggtcgacccg gacccggaga agatctggta
     8521 ccgctccaac ctcgaccagt cgctcgcctt cgagtcccgg tgggaggact tccggcgctg
     8581 ggtggcccgg cacgacgacg tctacgggct cggcgcctcc cccgaggccg tacagcgtca
     8641 ctacgacgac acgcgcaccg cgctcgcggc cgagccggcg ggcgggacgg tggggccggg
     8701 gcagctccac gcggggttcc tccaggccgc gtactacgac gactcctggg cgatgcgggc
     8761 gacggccctc tccgagtacg tccgggggaa cccggagcca ctggtcacac agctgtcgcc
     8821 gcgcgcggtc acggcgcggg acgacgagaa cgagcgggcc gtctacaccg ccgtcgagtg
     8881 caacgacgcg ccgtggcccg aggagtggga ggtctgggac cgcgaccaca cggacctcgc
     8941 ccgcatcgcg cccttccaga cctggcacaa cgccttcacc aacctgccgt gcgccttctg
     9001 gcccgcgccc cggcagcggc ccctggacgt ctccaccggg tggggcgagc tgccgccggt
     9061 gctgatcctg gcggcggagc gcgacgcggc gaccccgtac cagggcgcgc tggagcttca
     9121 gcggcggctg cgcggcgcgg tgctcgtgac ggagcgggga gcggggacgc acggtgtggg
     9181 cgggttcggg aacgcctgcg tggacgagca catgcggcgg tatctgctga cgggcgagac
     9241 gcccgccggg gacgcggagt gcgctccgcg cgcggagccg aaccccgtct cgctggacta
     9301 ggacctgccg ttgatgggga aacgggccct gtgggcgcac tctcggtgcg cccacagggc
     9361 ccgaagccga acccggtgtc gctggaacgg cgggcggcac ggtcggtgcc cggtgcccgt
     9421 cttctgatct tccgggttgg ggtggtgcgg gaactcgctg gctatgcgag cccggccacc
     9481 aggtctgcga tgtcctttcg gcggcccgtg tagaacggga tctcctcgcg cacgtggagc
     9541 cgtgcctcgg aggcgcgcag gtggcgcatc aggtcgacga tgcggtacag ctcgtccgcc
     9601 tcgaaggcga ggatccactc gtagtcgccc agggagaagg aggcgacggt gttggcgcgg
     9661 acgtccgggt agccccgggc catcttgccg tggtccgcga gcatgcggcg gcggtcctcg
     9721 tcgggcagca ggtaccagtc gtaggaccgg acgaaggggt agacgctgat gtagtcgcgc
     9781 ggggtctcgt cggcgaggaa cgccgggatg tgcgacttgt tgaactcggc gggccggtgc
     9841 agggccatgt tcgaccagac cggttcgaga gcgcgcccga gctcggtgcg gcggaagagg
     9901 ttgtaggcgt cctggagctg gtcggcggtc tcggcgtgcc accagatcat caggtcggcg
     9961 tcggcgcgga gaccggagag gtcgtaggtg ccgcgcacgg tgacgtcctt ggccgcgagc
    10021 tggtcgaaca gctcctggac ctcgtccgcg tatccggtgc ggtccagcgg cagcacgtcg
    10081 cggagtttga agaccgacca gagggtgtag cggatgacct cgttgaggtc cttggccttc
    10141 tttccggcgt tcggactctt gctgggtgtc acagtctcag gagcactcat acggctattg
    10201 tcccgcctcg ctcccggtgc cccgaaccag ggtcggcgtg gcgatgatct cgctcgtgat
    10261 cttccccgcc gcatcgcccg tggtcctccc cgtcaggtcc cgggcgatct cgtcggccgc
    10321 ccgctgcgcg ctcgcgatac aggcggggat gccgaccccg tcgtaggccg cgccgcagac
    10381 gcggagcgcg ggcagcttcg cgacctcgtc gcggatgcgg gcgacgcggg cgtggtggcc
    10441 gaccgggtac tgcgggaggc cgccgatcca gcgggtgacc tcggtgtcga cggggcgggc
    10501 ggcaagcccg gtggcctcgc gcaggtcccg cagggagacg gcgacgagtt cgccgtcctc
    10561 gcggtgcagg tggtcctcct cgccgtagcg gccgacggag gtgcggagca cgaagaggcc
    10621 gggggcggcg tccgcaaccc actgccactt gttgctggag aaggtggagg ccttgatggt
    10681 gcggccgtcg accggtggta cgaggaagcc ggagcggccg cgcagggcct tgagaccctc
    10741 gatgtcgacg cgccggaagg ccatggtgac cagggccatc gaggcgtact cgacaccggt
    10801 cagcccggtg gcggcggccg gggactcggc ggcgagcagg gcggaggcgg accaggcggg
    10861 ggtggcgagg acgacgccgt cggcggcgat cgtgccggcg tcggtgcgga ccgtccagcc
    10921 cgtggccgta cggctcaggc cgaggaccgg ggtctcggtc aggatctcgc cgcccccggc
    10981 ccgtacggcg tcgcccacgg ccccgggcag ggtgccgatg ccgccccgga ggccctggaa
    11041 gacggggccg gtctgctgcc gggccgcggc gcgctcctgg atgcggcgga cggcctccag
    11101 cagcgggccg ccctcctgca cggcttcgaa gagctgcggt acggcggcgc gcagcgagat
    11161 ccggtaggcg tcgcccgcgt agaccccgcc gaggaggggt tccaccagcc ggtcgacgac
    11221 ctcgcggccg agccggtccg ccacgtacgc gccgacggcc acgtcgtccc cgacgggcgt
    11281 cggggtcagg tcgcgctcct gggcgatccg ggccagcccc tcgggggaga gcacgccctc
    11341 cagggcggcc gggtcgccgg ggacgcccat gacgtggccc ttgggcatcg ggcgcagcgc
    11401 gtcgcgggtc cagagggagg cggtggcggt ggcgggcggc tggaggcggc cggagagtcc
    11461 gacggcctcg gccagggcga ccgcctcggg g
//
