LOCUS       NZ_KB890927            15385 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. CcalMP-8W B053DRAFT_scaffold_2.3, whole genome
            shotgun sequence.
ACCESSION   NZ_KB890927
VERSION     NZ_KB890927.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. CcalMP-8W
  ORGANISM  Streptomyces sp. CcalMP-8W
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 753305)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   2  (bases 1 to 753305)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. CcalMP-8W
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:14:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,603
            CDSs (total)                      :: 6,529
            Genes (coding)                    :: 6,424
            CDSs (with protein)               :: 6,424
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 1 (5S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 83 of 105
            Pseudo Genes (internal stop)      :: 5 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:04:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 263722
            Orig. end                         :: 279107
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB890927.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405361
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..15385
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /candidate_cluster_numbers="3"
                     /contig_edge="False"
                     /product="ectoine"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..15385
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /detection_rules="AfsA"
                     /kind="neighbouring"
                     /product="ectoine"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="2"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5375
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(8..961)
                     /locus_tag="B053_RS1000000137895"
     CDS             complement(8..961)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="B053_RS1000000137895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_078507703.1"
                     /transl_table=11
                     /translation="MQERAARTRQTVLEAAADEFAERGYEGASLQRVAWRAATSIGALT
                     FHFRNKTALADEVRTTGRDRFGRCLAELAPAADPLRELRTLIATLARLAHEDRFVRAAR
                     RLEADPPEGAPPLAETWLPVLRDLLDRAHGAHRLRPGVAPEDAVAMLAHLAEGAMAAHG
                     TPRDAAWDTLWDSVWDVVLHGLAADRGEGDGGDERDGRGDRTDGGDPGDPGEHGGRGDR
                     TDGDDQGVRGARGDRGDRRDADDRGNRGGRAGRGAGDDQGAGGGPGGRGGRAGRGAGDD
                     RGAGDGPGGRGGRRDRGAASGDASPDMTGASRRGRG"
     misc_feature    complement(722..724)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1083..2186
                     /locus_tag="B053_RS0115620"
     CDS             1083..2186
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452971.1"
                     /locus_tag="B053_RS0115620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_202439514.1"
                     /transl_table=11
                     /translation="MHETPEMATGRALDDLSRLLFGHLHRSDQQRWGHVYLQGLLRTHG
                     KKTVRRMAATVTGSEAASSSLHQFVNASPWEWTPVRGRLARWTARTLPVRSWTLAPTVL
                     PKRGDHSPGVHQRFLPESGRTVNCQLGLGLFLDAGQAHIPVDWQLFLPPRFTRDPAVRD
                     RAKIPATAQARPLWDQALHLVQEMTSWTDDGRTAPVVADLTALCDIAPLAQRLQGSGHA
                     FVLAIPGQTALGGPELPPGERPSARALLLRRGVRHPAGAGQQIISYETPVRGVRPSGEH
                     TQGAGRLMAEWDAGTGRPGRIWLTNITDRHPAELLELARSPRGAQSALRRMGEDFGLLD
                     FEGRSFPGWHRHMTLVSAAYACGTLEA"
     misc_feature    1191..1193
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2208..2223
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.67"
                     /tool="antismash"
     misc_feature    complement(2210..2223)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.24"
                     /tool="antismash"
     misc_feature    2266..2279
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.74"
                     /tool="antismash"
     gene            2317..2760
                     /locus_tag="B053_RS0115625"
     CDS             2317..2760
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489542.1"
                     /locus_tag="B053_RS0115625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_018489542.1"
                     /transl_table=11
                     /translation="MTINQQPRAASDQTMWEAVLGLHAFVERQLAHTLQRRYGVGLSEY
                     RALETLHQAENGECRMQELADHIGLGQSSVTRLVGRLDSAGYAYKDSCTDDKRGVFAVI
                     TEEGRKRYQEARATYADVLSSALNTASADAQLARAVQALRSAA"
     misc_feature    complement(2853..2868)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2886..2899)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            3102..4550
                     /locus_tag="B053_RS0115630"
     CDS             3102..4550
                     /NRPS_PKS="Domain: AMP-binding (1-382). E-value: 3.1e-58.
                     Score: 189.2. Matches aSDomain:
                     nrpspksdomains_B053_RS0115630_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060210.1"
                     /locus_tag="B053_RS0115630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_018489543.1"
                     /sec_met_domain="AMP-binding (E-value: 3.4e-59, bitscore:
                     195.8, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQEQPDSTALVASGERVTYAELERLTRLAAADLDGAGFGFVRALC
                     VPAHKSPETIALLLAAWRQGINTLVPSPSLGAAALATLTAQAHGPLTATAVPGGGVILA
                     REETDPALADGFVVPDPAESLLTLTTSGSTGVPKLVPIRTEAFDRFADWATGKFGLDGS
                     TRALSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAGYLRELALAHEVTLVQGVPL
                     LHRLLAADGARYPAVRTVVFTGEAVSEQGLRDAFAACPAARFHNVFGCTETNDSFIEDV
                     DPDAYVHPLPIGTPIDGTDAVLLIDGPGGTEVLQGPGTGELLVHTPFQTGGYLEQARNA
                     HAFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVRGVRTNTLEVETVLGAHP
                     DVVEACVVALPCPEAGNRLHAEVVRRDGASLSSLGLRAYAAKHLPRHAVPSSVGLAGTP
                     LPKTPTGKPDRNSIKNTRLNGDAP"
     aSDomain        3105..4247
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B053_RS0115630_AMP-binding.1"
                     /evalue="3.10E-58"
                     /label="B053_RS0115630_AMP-binding.1"
                     /locus_tag="B053_RS0115630"
                     /protein_end="382"
                     /protein_start="1"
                     /score="189.2"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="QEQPDSTALVASGERVTYAELERLTRLAAADLDGAGFGFVRALCV
                     PAHKSPETIALLLAAWRQGINTLVPSPSLGAAALATLTAQAHGPLTATAVPGGGVILAR
                     EETDPALADGFVVPDPAESLLTLTTSGSTGVPKLVPIRTEAFDRFADWATGKFGLDGST
                     RALSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAGYLRELALAHEVTLVQGVPLL
                     HRLLAADGARYPAVRTVVFTGEAVSEQGLRDAFAACPAARFHNVFGCTETNDSFIEDVD
                     PDAYVHPLPIGTPIDGTDAVLLIDGPGGTEVLQGPGTGELLVHTPFQTGGYLEQARNAH
                     AFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVR"
     CDS_motif       3483..3527
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B053_RS0115630_0001"
                     /evalue="4.90E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="B053_RS0115630"
                     /protein_end="142"
                     /protein_start="127"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LTTSGSTGVPKLVPI"
     CDS_motif       4218..4277
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B053_RS0115630_0002"
                     /evalue="5.80E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="B053_RS0115630"
                     /protein_end="392"
                     /protein_start="372"
                     /score="18.9"
                     /tool="antismash"
                     /translation="GRTDFQVKVRGVRTNTLEVE"
     cand_cluster    4504..15385
                     /candidate_cluster_number="3"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    4504..15385
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[9503:10385](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(9504..10385)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="2"
     gene            4547..4807
                     /locus_tag="B053_RS0115635"
     CDS             4547..4807
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763164.1"
                     /locus_tag="B053_RS0115635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_018489544.1"
                     /sec_met_domain="PP-binding (E-value: 5.8e-06, bitscore:
                     22.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTGAHDPIRAYIVNEFLAGEDAEDLTDDFDLIESTVVNSLGLVR
                     LISWISETYHIPVDDIPLEPAAYRTLADIRGFVARAAPASV"
     gene            5001..5375
                     /locus_tag="B053_RS0115640"
     CDS             5001..5375
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452967.1"
                     /locus_tag="B053_RS0115640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_018489545.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-25, bitscore:
                     85.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDVPAVEWGSGTSHRLLTEADGMGFTVCETLVRRGTTSG
                     LQYLGHLEACYCIGGSGEVVTTDGTRHKIKTGTIYALDQHDAHHLIASDDEDLLLVSVF
                     SPALRGDEKHSLSEDGFSRY"
     gene            5468..6598
                     /locus_tag="B053_RS0115645"
     CDS             5468..6598
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489546.1"
                     /locus_tag="B053_RS0115645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_018489546.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2.5e-18, bitscore:
                     62.2, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 6.2e-27, bitscore:
                     89.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 7e-34, bitscore:
                     112.6, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAEKDTDTYGITAPYGDELNDDVARWDRAGEFPQEKWKPLQRSG
                     LFTLPFAPEYGGAGRTLTETMAALEGLGHTSRDAGLNFSASTQIVSVGIPLQAFGSEEL
                     RSRHLPRVTSGEAITAHAITEPGHGSDVMNIRTTAVREGDEYVIDGGKMFITNAPVADL
                     FLLYVRTGKPGAFGLSTFLAERNTPGLRIGEPLETMGLRTSPISEVTLDNVRLPVANRL
                     GSEGAGFLIMDYVMKREVLFSFAVNLGEMTHRLKRVVEHATTRQQFGTAIGKNQAVSHK
                     IADMKIAVETARKWLYDTGRKVEQGTDASLDVAATKIVVSEANQRTAQHAIQIFGARGY
                     LTDTGIERELRNATSGSIYSGTNEIQRNCIAALMGL"
     gene            6614..7333
                     /locus_tag="B053_RS0115650"
     CDS             6614..7333
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845019.1"
                     /locus_tag="B053_RS0115650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="WP_018489547.1"
                     /transl_table=11
                     /translation="MTETLTDAVPAPPAAEDLDRLTAATDFLDHDHETVRAFVDKALQG
                     VDRATATPVELAVALYYAVRDGIHYEVYGADLSPQGLKASSIITGGKGFCLHKSVLYAA
                     ACRSVGIPARLHYSDVRNHLASDRLRSYIGGDVFFHGLATVYLDGRWLQVTPVFNKLLC
                     RLYGMTPLEFDGRSDSLYHPFDAQGRQSMEFLTDHGDFDDVPYEFVMGNMRRKHPKFLD
                     AEGTGTVSGGSLADEAS"
     gene            7566..8381
                     /locus_tag="B053_RS0115655"
     CDS             7566..8381
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763169.1"
                     /locus_tag="B053_RS0115655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_018489548.1"
                     /transl_table=11
                     /translation="MTQFQAREDGWGAVARVGVVVPHADVGPESELQAMAPRDVYIHGS
                     RVHFGAMRPGGEMDPKIPHDPVRAFIDPPFIDDAVELLAASPLDSIALGFTSSAYVLGA
                     DGEQKLFERLAERTRGIPLTGTTHAAAAGFRALGAERIALVNPPWFDDELSALGASYFG
                     ERGFDVVHHAPCGLPSNQKLITPEALVQWIESTVAPQRPQAVLVAGNGIRAVGTIAGLE
                     ERIGLPVLTANQVLFWHALHLAGGTGAAARISDYGALFGVRPEPGSVTG"
     gene            8378..8620
                     /locus_tag="B053_RS0115660"
     CDS             8378..8620
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489549.1"
                     /locus_tag="B053_RS0115660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6253 family protein"
                     /protein_id="WP_018489549.1"
                     /transl_table=11
                     /translation="MSLLPAYGYQAAVQETCDTPEYHMPLVCWHDDANGVRGLVLYRGA
                     LRPAEQVPGFRRYLAAEGLEQYATTGPWPLLAATA"
     gene            8676..9422
                     /locus_tag="B053_RS0115665"
     CDS             8676..9422
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: ACPS (132-191). E-value: 6.1e-09. Score:
                     27.7. Matches aSDomain:
                     nrpspksdomains_B053_RS0115665_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763171.1"
                     /locus_tag="B053_RS0115665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_018489550.1"
                     /transl_table=11
                     /translation="MTHDLKWSDAPPGEIRDRVAVWIVRAPAGQRLPPDGSPPDWLDEK
                     ERSRLAAIPQHSGKEAYAFAHWALRSVLGSSLSCPPRSVGFLRQPCPGCGGAHGRPAIR
                     VPDRGGEPPEFSMSHSGDHVAIAVAEATIGIDIESHPTPESVAGYLPFLHPREQQWLGG
                     RPDDELVRDFARLWTRKEAMAKATGQGMAAVMNRLDLTGQPLGWRVRQLIAPPGYEASF
                     AVPASVHLEVVMHDELPYPMPELEQV"
     aSDomain        9072..9248
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B053_RS0115665_ACPS.1"
                     /evalue="6.10E-09"
                     /label="B053_RS0115665_ACPS.1"
                     /locus_tag="B053_RS0115665"
                     /protein_end="191"
                     /protein_start="132"
                     /score="27.7"
                     /tool="antismash"
                     /translation="IGIDIESHPTPESVAGYLPFLHPREQQWLGGRPDDELVRDFARLW
                     TRKEAMAKATGQGM"
     gene            complement(9504..10385)
                     /locus_tag="B053_RS0115670"
     CDS             complement(9504..10385)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452960.1"
                     /locus_tag="B053_RS0115670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_106669362.1"
                     /sec_met_domain="AfsA (E-value: 5.5e-24, bitscore: 79.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHQWQVHKLRGEEVLLSTWTEPCPGRFVITARWPKGHAFYRPSQG
                     AYDPLLLAETVRQAVPLLSHAVHGVPREHRQAWEYVSFAVEPLASLVTTAEDEVRLVVT
                     CSNVVRRGTRFAGMTMDIDVHLGKRLMGIAHTRFNNQPEAIYRRLRGSYADIHTALEQC
                     LPAGPPVEPHRVGRCSPDDVVLSPALGGERPGRWLLRVDPAHPVLFDHPVDHVPGMLLL
                     EAARQAAHAVDGTAGAAVIGMRTDFVRYVELDAPAVVVAGPPVPAGTGRRRVPVSVEQH
                     GVRVFTSEVTLA"
     misc_feature    10565..10582
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.91"
                     /tool="antismash"
     misc_feature    10693..10708
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            10724..11356
                     /locus_tag="B053_RS0115675"
     CDS             10724..11356
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452959.1"
                     /locus_tag="B053_RS0115675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_018489552.1"
                     /transl_table=11
                     /translation="MAQPKQERAEQTRRALLHAAAEVFDEYGYAGASITRILKRAGVTA
                     GALYFHFGSKEDLAKAVMNSQPETLVPLVAAGGLQRLIDLTLVWSWQLQRDPLLRAGVR
                     LTNEQTSTEGPANADPYEKFRAIMTGYLEEAQEDGELQPGVDPAVVAEFVVAACTGMQM
                     YSNVVSGRRDLPERTQQMWNLILPGIAVPSVITRAEATPARGAAMMP"
     misc_feature    complement(11471..11485)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            11569..12288
                     /locus_tag="B053_RS0115680"
     CDS             11569..12288
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763173.1"
                     /locus_tag="B053_RS0115680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018489553.1"
                     /transl_table=11
                     /translation="MLSLRLAALNIDGVLLNDTFSPVIHQFVTSRGGTYSAALERSVFS
                     QPRHIAGRRLAAAVGGGMTGEAALAAYFEERAAHVDRHPVMITPGTRQLLHRLRSAGLR
                     TVCYGGLGKEHFDHHLGRYAHLFDGPGYVCTNDFRPGLREITEDVFGLRPAQALFVDDV
                     ARVADAARELGTAFVGRPSAFAHSHQRALMREAGVRHLIDRLDTLDDGLLRRIDQEMNA
                     DTFWSTPLTAAGRGGYA"
     misc_feature    11578..11580
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            12288..13118
                     /locus_tag="B053_RS0115685"
     CDS             12288..13118
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (31-201). E-value: 5.2e-14.
                     Score: 44.5. Matches aSDomain:
                     nrpspksdomains_B053_RS0115685_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763174.1"
                     /locus_tag="B053_RS0115685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_018489554.1"
                     /sec_met_domain="adh_short (E-value: 4e-38, bitscore:
                     126.5, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.4e-30, bitscore:
                     102.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGGGPQSGRATAGGPRSGRAAPGGGLLSGKVALVTGASSGIGAA
                     SARHFADEGAAVVLVARRAALLEEGAERIRKDGGRAVAVPGDVTRPADMERAVATAVER
                     FGRLDCAFNNAGHASAGTLLHELPDDVFDRTLDVNLRGVWNCLRAQLPALLAAGGGAVV
                     NTSSVAGIRATAASAAYVAAKHAVIGLTRAAAAEYGEHGIRVNALVVGGTDTEMMDALR
                     PPGPAAGAGFGSQGIQRRLADPVEIARAAAWLCSDASSFTTGTALAVDGGRTAK"
     aSDomain        12381..12890
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B053_RS0115685_PKS_KR.1"
                     /evalue="5.20E-14"
                     /label="B053_RS0115685_PKS_KR.1"
                     /locus_tag="B053_RS0115685"
                     /protein_end="201"
                     /protein_start="31"
                     /score="44.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALVTGASSGIGAASARHFADEGAAVVLVARRAALLEEGAERIRK
                     DGGRAVAVPGDVTRPADMERAVATAVERFGRLDCAFNNAGHASAGTLLHELPDDVFDRT
                     LDVNLRGVWNCLRAQLPALLAAGGGAVVNTSSVAGIRATAASAAYVAAKHAVIGLTRAA
                     AAEYGEH"
     CDS_motif       12387..12476
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B053_RS0115685_0001"
                     /evalue="6.20E-07"
                     /label="PKSI-KR_m1"
                     /locus_tag="B053_RS0115685"
                     /protein_end="63"
                     /protein_start="33"
                     /score="22.1"
                     /tool="antismash"
                     /translation="LVTGASSGIGAASARHFADEGAAVVLVARR"
     gene            complement(13271..13687)
                     /locus_tag="B053_RS0115690"
     CDS             complement(13271..13687)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763175.1"
                     /locus_tag="B053_RS0115690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_018489555.1"
                     /transl_table=11
                     /translation="MSNAVKNPTNAWLSLLLAGVFEIGYALAVGGSEGFTVLTWSLVAV
                     VFFLLTLYALSLALRTIEVSIGYAVWAGIGAVGAAVLGPVFFDETLTLAKGIWLAVIIV
                     GVIWLKLADRTKPEAVAPAPVAQQVTDRPGALQV"
     gene            complement(13769..14089)
                     /locus_tag="B053_RS0115695"
     CDS             complement(13769..14089)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967532.1"
                     /locus_tag="B053_RS0115695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_018489556.1"
                     /transl_table=11
                     /translation="MHWLYLAIAVAFEIAVAIAAGKAEGFKNRKWTGITLASGAAATFF
                     LSKALLTFDVGVGYALWTSVAGVGITILGALFFGQRLNWNKAIGIVLVIGGVVGLQLSG
                     SA"
     gene            complement(14255..14734)
                     /locus_tag="B053_RS0115700"
     CDS             complement(14255..14734)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1240: riboflavin biosynthesis protein RibD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763177.1"
                     /locus_tag="B053_RS0115700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside deaminase"
                     /protein_id="WP_018489557.1"
                     /transl_table=11
                     /translation="MGTHDSELMGFAHEAIRLSREHVAQGGIPFSGVVVGGGRILGTGF
                     NRVREDNDPTAHAEVVALREATTRHGIRAVAGATLIASGEPCALCYMAARFAGIGHIVH
                     TADRRTAARYGFDYTSTYSLFAEDPERWPIKVTALQLPEAEAPFQDFLTMSRTRH"
     misc_feature    complement(14772..14785)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    14839..14852
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.74"
                     /tool="antismash"
ORIGIN
        1 gacgggctca gcccctgccg cgcctcgagg ctccggtcat gtccggcgac gcgtcccccg
       61 aggccgcccc ccggtcacgc cggccgcccc ggccacccgg cccgtccccg gcaccccggt
      121 cgtccccggc accccgcccc gcgcgaccgc cccggccacc cggcccgccc ccggcaccct
      181 ggtcgtcccc ggcaccccgc cccgcgcgac cgccccggtt cccccggtcg tccgcgtcgc
      241 gccgatcgcc tcggtcgccc cgggctcccc ggaccccctg gtcgtcccca tcggtccggt
      301 caccccggcc accgtgctcg cccgggtcgc ccgggtcgcc cccatcggtc cggtcgcccc
      361 ggccgtcccg ctcgtccccg ccgtccccct ccccccggtc cgcggcgagg ccgtgcagga
      421 ccacgtccca gacggagtcc cagagcgtgt cccaggccgc gtcgcggggg gtgccgtggg
      481 cggccatcgc gccctccgcg aggtgggcca gcatcgccac cgcgtcctcg ggcgccacgc
      541 cggggcggag ccggtgggcg ccgtgggccc ggtcgagcag atcgcggagg acggggagcc
      601 aggtctcggc caacggcggt gcgccttccg gcggatcggc ctccagacgg cgtgcggcgc
      661 ggacgaagcg gtcctcgtgc gccagccggg ccagcgtggc gatcagggtg cgcagctccc
      721 gtaacggatc ggcggcgggg gcgagttccg ccaggcagcg gccgaagcgg tcgcgcccgg
      781 tggtgcgcac ctcgtcggcc agggcggtct tgttgcggaa gtggaaggtc agagcaccga
      841 tcgaggtcgc ggcccgccag gccacccgct ggagggaggc gccctcgtaa ccgcgctcgg
      901 cgaactcgtc ggccgccgcc tccagcacgg tctggcgcgt cctggccgcc cgttcctgca
      961 tgcgccttct ccttgaacct cggtaagtcg tcgcgcttct ccctcagaaa tataacgaac
     1021 attctctatg tttgttagtc tgctcagcga cgctcacctg ggagggacct gccgtgacga
     1081 cgatgcacga aacgcccgag atggcgacgg gccgagcgct ggacgacctg tcccgcctcc
     1141 tcttcggcca tttgcaccgc tccgaccagc agcgttgggg ccatgtgtac ttacaggggc
     1201 tgctgcgcac gcacggcaag aagaccgtgc gccgcatggc ggccaccgtg accggatccg
     1261 aggcggcgtc gtcgtcgctg caccagttcg tgaacgccag cccctgggag tggaccccgg
     1321 tccgcggccg gctggcccgc tggaccgccc ggacgctgcc ggtgcggagc tggaccctgg
     1381 cgccgaccgt gctgcccaag cggggcgacc actcgcccgg agtgcaccag cgcttcctgc
     1441 cggagtccgg gcgcaccgtc aactgccagc tgggactggg cctgttcctg gacgcggggc
     1501 aggcgcacat cccggtcgac tggcagctct tcctgcctcc ccggttcacc cgggaccccg
     1561 ccgtacgcga ccgggcgaag atccccgcga cggcccaggc ccggccgctg tgggaccaag
     1621 ccctgcacct ggtccaggag atgacgtcct ggacggacga cgggcggacc gcacccgtgg
     1681 tggccgacct gacggccctg tgcgacatcg caccgctggc acagcggctc cagggcagcg
     1741 ggcacgcctt cgtgctcgcc atcccggggc agaccgccct cggcggaccc gaactgccgc
     1801 cgggcgagag gccgtcggcg cgcgcgctgc tgctgcgccg cggggtgcgg cacccggcgg
     1861 gcgcgggcca gcagatcatc tcgtacgaga cccccgtgcg cggggtgcgc ccgagcggtg
     1921 agcacaccca gggggcgggc cggctgatgg ccgagtggga cgcgggcacc ggacgcccgg
     1981 gccggatctg gctgacgaac atcaccgacc gccatccggc cgaactgctg gagctggccc
     2041 gctcgccccg gggggcgcag tccgccctcc ggcgcatggg cgaggacttc gggctcctgg
     2101 acttcgaagg ccgctccttc cccggctggc accggcacat gacgctcgtc tcggccgcct
     2161 acgcgtgcgg caccctggag gcatgacccc ccgacgattt acttgcgcat acaaataaat
     2221 atcggccaga gcccaccccg tgggctccgg cactccccag atgattgcat gggcatgtat
     2281 cttgttgtta tcgcagtcga gaaggggagc tgttccatga cgatcaacca gcagccacgc
     2341 gccgcctccg atcagaccat gtgggaggcc gttctcggcc tgcacgcctt cgtggaacgc
     2401 cagctcgcgc acaccctcca gcgccgctac ggcgtcggcc tgtccgagta ccgggccctg
     2461 gagaccctcc accaggccga gaacggcgag tgccgcatgc aggagctggc ggaccacatc
     2521 ggcctgggcc agagttcggt gacgcgcctg gtggggcggc tcgacagcgc cggatacgcc
     2581 tacaaggaca gctgcaccga cgacaagcgc ggggtcttcg ccgtcatcac cgaagaaggc
     2641 cgcaagcgct accaggaagc ccgcgccacg tacgccgacg tcctcagctc ggccctcaac
     2701 accgcgagcg ccgacgccca gctcgcccgg gcggtgcagg cgctgcgcag cgccgcctga
     2761 cacgatcacg ccagacggcg ggaggcggcc ggggtgaccc ggcgcccccg ccgtcttttg
     2821 catgttcggg cttcttccac aaccggcgcg ggtgtggcct ggatcacacg aacgctctat
     2881 ctatttgctt gaccatgcaa ataaagaccc gtagattctc tctcgtagcc agatctcgcg
     2941 gcctcagggc cggctcatcc ggctcaggcc cgatcgcgta cccggctcgc acagctcgat
     3001 cgcgcagccg gaccgcacag ccggatcctg ccgccagatc gcccaaccac cgagggggac
     3061 cgttgccatc gcctcgcctg ttacaggcat tcagcagaca tgtgcaggaa cagcccgaca
     3121 gcaccgcgct cgtcgcctcc ggcgaacggg tcacctacgc ggagctggag cggctgaccc
     3181 gcctcgccgc cgcggacctg gacggcgccg gcttcggatt cgtccgcgcc ctgtgcgtac
     3241 cggcccacaa gtcgccggag accatcgccc tgctgctggc ggcctggcgc caggggatca
     3301 acaccctggt cccctccccg agtctgggcg ccgccgccct ggccacgctc accgcccagg
     3361 cccacggccc gctgacggcc accgcggtcc ccggcggcgg cgtgatcctg gcgcgggagg
     3421 agaccgaccc ggccctggcg gacggcttcg tcgtccccga ccccgccgag tcgctgctga
     3481 cgctcaccac ctccggttcc accggtgtcc ccaagctggt cccgatccgc accgaggcct
     3541 tcgaccggtt cgccgactgg gccaccggga agttcgggct ggacggctcc acccgggccc
     3601 tgagcttctc gccgctcaac ttcgatctgg cgctcctgga cgtctggacg gtcctggagg
     3661 ccggcggcac ggtcgtgctg gccgacgccg gggccagcgg ggacgccggc tatctgcggg
     3721 agctggccct cgcccacgag gtgaccctcg tgcagggcgt gcccctcctc caccggctgc
     3781 tggccgcgga cggggcccgc tacccggcgg tccgcacggt cgtcttcacc ggggaggccg
     3841 tctccgagca gggactgcgg gacgcgttcg ccgcctgccc ggccgcccgc ttccacaacg
     3901 tcttcggctg caccgagacc aacgacagct tcatcgagga cgtcgacccg gacgcgtacg
     3961 tccatccgct gcccatcggc accccgatcg acggcaccga cgcggtgctg ctgatcgacg
     4021 ggcccggggg caccgaggtc ctccagggtc ccggcaccgg tgaactgctg gtgcacaccc
     4081 ccttccagac cggcggctac ctcgaacagg cccgcaacgc ccacgcgttc atgcccgacc
     4141 cgcgcggcgg cggcgccatg ttctaccgca ccggcgacct cgtccaccgc gacgccgacg
     4201 gccgctactt cctccagggg cgcaccgact tccaggtcaa ggtacgcggc gtgcgcacca
     4261 acacgctgga ggtcgagacc gtcctcggcg cccatccgga cgtcgtcgag gcctgtgtcg
     4321 tcgccctgcc ctgcccggag gccggcaacc ggctgcacgc cgaagtggtg cgccgggacg
     4381 gggcctccct cagctcgctg ggactgcggg cgtacgcggc gaagcacctg ccgcgccacg
     4441 ccgtgcccag ctcggtcggg ctcgccggca ccccgctgcc gaagaccccg accggcaagc
     4501 ccgaccgcaa ctcgatcaag aacacccgac tgaacggaga cgccccatga gcaccggtgc
     4561 ccacgacccg atccgcgcct acatcgtcaa cgagttcctc gccggcgagg acgccgagga
     4621 cctgaccgac gacttcgacc tcatcgagag caccgtcgtc aacagcctcg gcctggtccg
     4681 cctcatctcc tggatatcgg agacgtacca catccccgtc gacgacatcc ccctggagcc
     4741 ggccgcctac cgcacgctcg cggacatccg cggcttcgtc gcgcgggccg ccccggcctc
     4801 cgtctgaccc cacgagcccc accgccccat ccccgtacca cccctccccc cacgcgcgga
     4861 gaacgcgcac gcgcaggacg cgcacgcacg gaacgcacac gcacacgcac agaggcacga
     4921 acgcaccggc acacccgcac caggcgcacc agcacaggcc cagaacacgt ccagacaaca
     4981 gcgatgacag gagtactccc atgatcgtcc gttctctctc cgacgtcccc gcggtcgaat
     5041 ggggcagcgg caccagccac cgcctgctca ccgaggcgga cggtatgggc ttcaccgtct
     5101 gcgagacgct ggtccgccgg ggcaccacga gcgggctgca gtacctcggc cacctggagg
     5161 cctgctactg cataggcggc tcgggcgagg tcgtcaccac cgacggcacc cgccacaaga
     5221 tcaagaccgg gaccatctac gccctggacc agcacgacgc ccaccacctg atcgcctccg
     5281 acgacgagga cctcctcctg gtctccgtct tcagcccggc gctgcggggc gacgagaagc
     5341 actcgctctc cgaggacggc ttctcccgct actgatcccg ccgccgggtg cggggccgcg
     5401 acggcgcggc cccgcacccg gcccatcccg cgaccggcga accggcgaaa ggcaaggcag
     5461 agccctcatg gcagcagaga aggacacaga cacctacggc atcacggcgc cgtacggcga
     5521 cgagctgaac gacgacgtcg cccgctggga ccgggccggc gagttccccc aggagaagtg
     5581 gaagccgctc cagcgctccg gcctcttcac cctccccttc gccccggagt acggcggcgc
     5641 cggccggacg ctcaccgaga ccatggccgc gctggagggc ctcggccaca ccagccggga
     5701 cgcgggcctc aacttctcgg cgtccacgca gatcgtcagc gtcggcatcc cgctccaggc
     5761 gttcggctcc gaggagctgc gctcgcgcca tctgccgcgg gtcacctcgg gcgaggccat
     5821 caccgcgcac gcgatcaccg agccgggcca cggctcggac gtcatgaaca tccggaccac
     5881 ggccgtccgc gagggcgacg agtacgtgat cgacggcggc aagatgttca tcaccaacgc
     5941 ccccgtcgcc gacctcttcc tgctctacgt ccgcaccggc aagccgggcg ccttcgggct
     6001 cagcaccttc ctcgccgagc gcaacacgcc gggcctgagg atcggtgagc cgctggagac
     6061 gatggggctg cgcacctccc cgatcagcga ggtcacgctc gacaacgtcc ggctgcccgt
     6121 cgccaaccgg ctcggcagcg agggcgccgg cttcctgatc atggactacg tgatgaagcg
     6181 ggaggtcctc ttctccttcg ccgtcaacct cggcgagatg acccaccgcc tcaagcgcgt
     6241 cgtcgaacac gccaccaccc ggcagcagtt cggcaccgcc atcggcaaga accaggcggt
     6301 ctcccacaag atcgcggaca tgaagatcgc ggtggagacg gcccgcaagt ggctgtacga
     6361 caccgggcgc aaggtcgagc agggcacgga cgcctcgctg gacgtggccg ccaccaagat
     6421 cgtggtcagc gaggccaacc agcgcaccgc ccagcacgcc atccagatct tcggcgcccg
     6481 cggctatctg accgacaccg gcatcgagcg cgagctgcgc aacgccacct ccggctccat
     6541 ctactccggc accaacgaga tccagcgcaa ctgcatcgcc gccctgatgg gcctgtgacg
     6601 agaggcatcc gccatgaccg agaccctgac cgacgccgtc cccgcgcccc cggccgccga
     6661 ggacctcgac cgcctcaccg cggccaccga cttcctcgac cacgaccacg agaccgtacg
     6721 cgccttcgtg gacaaggccc tccagggggt cgaccgcgcc accgccaccc ccgtcgaact
     6781 cgccgtcgcc ctctactacg cggtgcgcga cggaatccac tacgaggtgt acggcgccga
     6841 cctgtcgccg caggggctga aggcgtccag catcatcacc ggcggcaagg gcttctgcct
     6901 gcacaagtcc gtcctctacg ccgccgcctg ccgctccgtc ggcatcccgg cccggctgca
     6961 ctacagcgac gtccgcaacc acctcgcctc cgaccggctg cgctcctaca tcggcggcga
     7021 cgtcttcttc cacggcctgg ccaccgtgta cctggacggc cggtggctgc aggtgacccc
     7081 ggtcttcaac aagctgctgt gccggctgta cggcatgacc ccgctggagt tcgacggccg
     7141 ctccgacagc ctctaccacc ccttcgacgc gcaggggcgg cagagcatgg agttcctcac
     7201 cgaccacggc gacttcgacg acgtccccta cgagttcgtc atgggcaaca tgcgccgcaa
     7261 gcacccgaag ttcctcgacg cggagggcac cggcacggtc agcggcggct cgctggccga
     7321 cgaggcgagc tgagccccgc cgcgtagagc ggcacgcccc ccataccggg cggcacgcca
     7381 tccccgtacc gggctcgcca tctccgtacc gggcacgccc cccacgtacc gggcacgcca
     7441 cccccgtaca ccggcacgcc ccacccgccc gctcacccgc tcgcccacag gcacgtcccc
     7501 ccgcacccag gcacgtcccg tcatgtcacg tcagcagcac ggcagaagaa gaggagaagc
     7561 accccatgac gcagttccag gcccgagagg acggctgggg cgccgtcgcc cgcgtcggtg
     7621 tcgtcgttcc gcacgcggac gtcgggccgg agtccgagct ccaggcgatg gccccgcgcg
     7681 acgtctacat ccacggctcc cgcgtccact tcggggcgat gcggcccggc ggcgagatgg
     7741 accccaagat cccgcacgac ccggtgcgcg ccttcatcga cccgcccttc atcgacgacg
     7801 ccgtcgaact gctcgcggcc tccccgctgg actccatcgc cctggggttc accagctccg
     7861 cctacgtcct gggcgccgac ggcgagcaga agctcttcga gcggctcgcg gagcggaccc
     7921 gcggcatccc gctcaccggc accacccacg cggccgcggc cgggttccgc gcgctgggcg
     7981 ccgagcggat cgcgctggtc aacccgccgt ggttcgacga cgagctgtcg gccctgggcg
     8041 cctcgtactt cggtgagcgg gggttcgacg tcgtccacca cgccccctgc gggctcccca
     8101 gcaaccagaa gctcatcacc ccggaggccc tggtgcagtg gatcgagtcc accgtcgccc
     8161 cgcagcggcc gcaggcggtc ctggtcgccg gcaacggcat ccgcgccgtg ggcaccatcg
     8221 cggggctgga ggagcggatc ggcctccccg tcctcaccgc caaccaggtg ctcttctggc
     8281 acgcgctgca cctggccggc ggcaccgggg cggccgcgcg gatcagcgac tacggcgccc
     8341 tgttcggcgt ccgcccggaa cccgggagcg tgacgggatg agcctgctgc ccgcgtacgg
     8401 ctaccaggcc gccgtgcagg agacctgtga cacgccggag taccacatgc ccctggtgtg
     8461 ctggcacgac gacgccaacg gcgtgcgcgg gctggtgctg taccgcgggg cgctgcgccc
     8521 ggcggagcag gtgccggggt tccggcgcta tctcgccgcc gagggtctgg agcagtacgc
     8581 cacgaccggc ccgtggccgc tgctcgcagc aaccgcctga tccgacccgt ggcggggacc
     8641 ggccgggtcc ccgccacatc aggagccgta tcgacatgac acacgacctc aagtggtccg
     8701 acgcgcctcc gggcgagatc cgcgaccggg tggccgtctg gatcgtccgg gccccggccg
     8761 ggcagcgcct gccgcccgac gggtccccgc ccgactggct cgacgagaag gaacggagcc
     8821 ggctggccgc gatcccgcag cactccggca aggaggccta cgccttcgcc cactgggcgc
     8881 tgcggagcgt cctcgggtcc tcgctcagct gcccgccccg gagcgtcgga ttcctgcggc
     8941 agccgtgtcc cggctgcggc ggggcgcacg gcagaccggc catccgcgtt ccggaccggg
     9001 gcggcgagcc accggagttc tcgatgtcgc acagcgggga ccacgtcgcc atcgcggtcg
     9061 ccgaggccac catcggcatc gacatcgagt cccatccgac cccggagtcg gtcgccggct
     9121 atctgccctt cctccacccg agggagcagc agtggctggg cggccgtccc gacgacgaac
     9181 tggtccggga cttcgcccgg ctgtggaccc gcaaggaggc catggcgaag gcgacgggcc
     9241 agggcatggc ggccgtcatg aaccggctgg acctcaccgg ccagccgctc ggctggcggg
     9301 tgcggcagct gatcgcccca cccgggtacg aggccagctt cgcggtcccg gcctcggtgc
     9361 acctggaagt cgtcatgcat gacgaactcc cttatccgat gccggagttg gagcaggtct
     9421 gaccagtggc gggcagtccg tacctctgtg gaggggaggt acgggccgcc cgccgcgtca
     9481 cgtcgtgggc ccggtggagc cggtcaggcg agggtgacct cggaggtgaa gacacggacg
     9541 ccgtgctgct cgacggagac ggggacgcgg cggcggcccg taccggcggg gaccggggga
     9601 ccggccacga cgacggcggg ggcgtcgagt tcgacgtacc ggacgaagtc cgtacgcatc
     9661 ccgatgacgg ccgcgccggc ggtgccgtcg acggcgtgcg ccgcctggcg ggcggcttcg
     9721 agcaggagca tcccgggcac atggtcgacc gggtggtcga agaggaccgg gtgggcgggg
     9781 tcgacgcgca gcagccaccg gccgggtctc tcgcccccca gcgcgggaga gaggaccacg
     9841 tcgtccgggg agcaccggcc cacccggtgc ggctccaccg gcgggcccgc cgggaggcac
     9901 tgctcaaggg cggtgtggat gtccgcgtaa ctcccgcgca gtctgcggta gatggcctcg
     9961 ggctggttgt tgaagcgggt gtgcgcgatg cccatcagcc gcttccccag gtggacatcg
    10021 atgtccatgg tcatcccggc gaacctggtg ccgcgccgga ccacgttcga gcaggtgacg
    10081 accaggcgga cctcgtcctc ggcggtggtg accagcgagg ccagcggctc gaccgcgaag
    10141 ctcacgtact cccaggcctg cctgtgctcc cgggggacgc cgtgcacggc gtgggagagc
    10201 agcggaaccg cctggcgtac ggtctccgcg agcagcagcg ggtcgtaggc gccctgcgac
    10261 ggccggtaga acgcgtgccc cttcggccag cgcgccgtga tcacgaagcg tcccgggcac
    10321 ggctcggtcc acgtgctgag cagtacttcc tcgccccgaa gcttgtgcac ctgccactgg
    10381 tgcaccggcg accgccggac cgccggccgc tcgaccggcg cctgccggac aggcgcctgc
    10441 cggaccggct gcgcgtcgcg tctcagcacg gaaacagaca acgtggaccc cccagatcgt
    10501 tagttgctcg tggacgacta cacagaggcg cgctcagggc actccatgca ccgacccaaa
    10561 aataatgttt cttttgtttt actgggtcaa ggggttgggc ggccaaggtt tcccctgagg
    10621 caagcagggc gcatacccag cccaactctg ctaaaagaca ctcaacttgg cgacgatcgg
    10681 gccaggtaac ataaggagca ttcttttcgg gaaggtgaag ggcatggcgc aaccgaaaca
    10741 ggagcgagcg gagcagaccc gtcgcgctct cctccatgca gcagcagaag tcttcgacga
    10801 gtacggctac gcgggagcca gcatcacccg catcctcaaa cgcgccggtg tgacggccgg
    10861 ggcgctctac ttccacttcg gctccaagga ggacctggcc aaggcggtga tgaacagcca
    10921 gccggagacc ctggtcccgc tggtcgccgc aggcgggctg cagcggctca tcgacctcac
    10981 gctcgtctgg tcgtggcagc tccagcgcga tccgctgctc cgggccggcg tacggctgac
    11041 caacgagcag acctccacgg agggccccgc gaacgccgac ccgtacgaga agttccgcgc
    11101 gatcatgacc ggctatctgg aggaggccca ggaggacggc gagctgcagc cgggtgtcga
    11161 cccggccgtg gtggcggagt tcgtggtcgc cgcctgtacc gggatgcaga tgtactccaa
    11221 cgtcgtcagc ggccgccggg acctccccga gcgcacccag cagatgtgga atctgatcct
    11281 cccgggcatc gcggtgccgt ccgtgatcac ccgcgccgag gcgacccccg cccggggcgc
    11341 cgcgatgatg ccctgaccgc accggtccac ggaccgggcc gaccgcccgc accgaccgat
    11401 cgccccgact cccccgaccg cacccgccga accgcactcc ggccacgggc gtcctgatca
    11461 cgggcgcccg tggccgagga cgtcccgacc gcacacgcgc ccgaccgccc cacgaccgct
    11521 ctgcccgccg accgttcccc ccgccccgga cgatccctac ggagccgcat gctttcctta
    11581 cgcctggccg cgctgaacat cgacggtgtc ctgctcaacg acaccttcag tccggtcatc
    11641 caccagttcg tcaccagcag gggcggtacg tactccgccg ccctggagcg atccgtcttc
    11701 tcccagccgc ggcacatagc cggccggcgg ctcgcggccg ccgtgggcgg gggcatgacc
    11761 ggcgaggcgg cgctcgccgc ctacttcgag gagcgcgccg cccacgtcga ccgtcacccg
    11821 gtcatgatca cgccgggcac ccgccagctg ctccaccggc tgcggagcgc gggcctgcgc
    11881 acggtctgct acggcggcct gggcaaggag cacttcgacc accatctggg ccgctacgcc
    11941 cacctgttcg acggccccgg ctatgtctgc accaacgact tccgccccgg cctccgggag
    12001 atcaccgagg acgtcttcgg cctccgcccc gcccaggccc tcttcgtcga cgacgtcgcc
    12061 cgggtcgcgg acgcggcccg cgagctgggc acggccttcg tcggccgtcc ctccgccttc
    12121 gcccacagcc accagcgcgc actcatgcgg gaggcgggcg tacggcacct gatcgaccgc
    12181 ctggacacgc tcgacgacgg cctgctgcgc cggatcgacc aggagatgaa cgcggacacc
    12241 ttctggagca cccccctgac cgcggcgggc cggggcgggt acgcgtgatg ccgggcggag
    12301 ggccgcagtc cggcagagcg acggcgggcg ggccacggtc cggcagagcg gcgccgggcg
    12361 gcgggctgct gtccggcaag gtggcgctgg tcaccggcgc ctccagcggg atcggcgccg
    12421 cgtcggcccg gcacttcgcc gacgagggcg ccgccgtcgt gctcgtggcc cgccgggccg
    12481 ccctgctgga ggagggcgcc gagcgcatcc gcaaggacgg cggccgggcc gtggccgtac
    12541 cgggcgacgt cacccgcccc gcggacatgg aacgggccgt ggccacggcg gtcgagcggt
    12601 tcggccgcct cgactgcgcg ttcaacaacg ccggtcacgc cagcgccgga acgctcctgc
    12661 acgaactccc cgacgacgtc ttcgaccgca ccctggacgt caatctgcgc ggggtgtgga
    12721 actgcctgcg cgcccagctc cccgccctgc tggcggcggg cgggggcgcg gtcgtcaaca
    12781 cctccagcgt cgcgggcatc cgggccaccg ccgcgtcggc cgcctatgtg gccgcgaagc
    12841 acgcggtcat cggcctcacc cgggcggcgg cggccgagta cggcgagcac ggcatccggg
    12901 tcaacgccct cgtggtgggc ggcaccgaca cggagatgat ggacgccctc cgcccgcccg
    12961 gccccgccgc gggcgcgggc ttcggctccc agggcatcca gcggcggctg gccgacccgg
    13021 tcgagatcgc ccgcgccgcc gcctggctct gctcggacgc ctcctccttc accaccggga
    13081 ccgccctcgc ggtggacggc ggccgcaccg ccaagtgacc gctgcggcac gcgacacgac
    13141 ggaacgcccc cggcggctgc cgcccattcc tgatgggagg cagccgcctg gggcgtgcgg
    13201 tcgtggtgcg tacggtggcg atggctgcgg ccgtggtggc tgcggccgtc gtggccacgg
    13261 tcgtggcgcg tcagacctgg agcgcgccgg gccggtccgt gacctgctgg gccaccgggg
    13321 ccggggcgac cgcctcgggc ttcgtgcggt cggccagctt cagccagatg acaccgacga
    13381 tgatgaccgc cagccagatg cccttggcga gggtgagggt ctcgtcgaag aagaccgggc
    13441 ccaggacggc ggcgccgacc gcgccgatac cggcccagac cgcgtagccg atgctgacct
    13501 cgatcgtgcg cagggccagg ctgagtgcgt agagcgtcag caggaagaac accacggcga
    13561 ccagggacca ggtcaggacg gtgaagcctt cgctgccgcc gacggcgagc gcgtatccga
    13621 tctcgaagac tccggcgagc aggagcgaga gccaggcgtt ggtggggttc ttgaccgcgt
    13681 tggacatggg aggtggatcc tctctgaaat ggcgtcagat ggtgtgcggg gagcgccgcg
    13741 tgcgcggccg ggggtggtgc acccgggctc aggcggaacc gctcagctgg aggccgacga
    13801 cgccaccgat gacgaggacg atgccgatcg ccttgttcca gttcaggcgc tggccgaaga
    13861 agagcgcgcc gaggatggtg ataccgacgc ccgcgacgga ggtccacagg gcgtagccga
    13921 cgccgacgtc gaaggtcagc agcgccttgc tgaggaagaa ggtcgcggcg gcgccgctgg
    13981 ccagggtgat gccggtccac ttgcggttct tgaagccctc cgcctttccg gcggcgatgg
    14041 cgacagcgat ctcgaaggcg acggcgatgg cgaggtagag ccagtgcatg ggaggtcctt
    14101 ccgtgcggtg ggtgaggagg ggagtgggcg tgggggaggc ggtgcgtgcg gcggtgtacg
    14161 acgatgtcgc ggggccgggc ccggggcccg ggccgggtcg gcaccgggct ccggcgggcg
    14221 ccggtcggtg ccggtgcccg ggccggagcc gtggtcagtg ccgggtccgg ctcatggtga
    14281 ggaagtcctg gaacggggcc tcggcctcgg ggagctggag cgcggtgacc ttgatgggcc
    14341 agcgctcggg gtcctcggcg aagagggagt aggtgctggt gtagtcgaag ccgtaccggg
    14401 cggcggtccg gcggtcggcg gtgtgcacga tgtggccgat gccggcgaac cgcgccgcca
    14461 tgtagcacag ggcgcagggc tcgccggagg cgatcagggt ggcccccgcc acggcgcgga
    14521 tgccgtgccg ggtggtggcc tcgcggaggg cgacgacctc ggcgtgcgcg gtcgggtcgt
    14581 tgtcctcgcg gacccggttg aagccggtcc ccaggatccg tccgccgccc acgacgacac
    14641 cggagaaggg gatgccgccc tgggcgacgt gctcgcggct gagccggatc gcttcgtggg
    14701 cgaagcccat cagttcgctg tcgtgtgtac ccatggcagt tcgtctcccg agttgccctg
    14761 tatcgttcat ttgcctgcac atgcaatcgg atggttccac aagtctccgc cgttgacgcg
    14821 acccgatgga cttaatcttg catgtgcatg taactggtgt caagcggttg cgtgcgtacg
    14881 ggcgttgtcc aaggtcgcca gcggttccag cacccgcgga tcgaacggca ccggaggaac
    14941 ccccgcggcc accttgcgcg gcaggtcggg gttggccagc gcgcccgtcc cgacggccag
    15001 gacgtcggcg tgcccctcgt ccaggacgcg ccgcgccacc gcgtcctcgt gcagagcgcc
    15061 gttggcgatg acggggagcc cggtgacccg ccgggcgagc ccggggagcg tcggcccgtc
    15121 cggcgaccag tcccgcccgc tgcccgcgag gtgcagatag tcggcaccgg caccggcggc
    15181 cgccgtgaag aggacgcgcg cctcggcgtc gccgcccggc caccgccacc cgaagtcgtt
    15241 gatcttcccc tgggagaacc ggacgccgac cgtgaagccg ggcccggccg cgcctctgac
    15301 cccggccgtc acctcccgca cgagccgcga gcgcccctcg gcgtccccgc cgtacccgtc
    15361 ggtgcgcaga ttggtgtacg gggtg
//
