LOCUS       NZ_KB890924            22797 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. CcalMP-8W B053DRAFT_scaffold_17.18, whole genome
            shotgun sequence.
ACCESSION   NZ_KB890924
VERSION     NZ_KB890924.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. CcalMP-8W
  ORGANISM  Streptomyces sp. CcalMP-8W
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 153122)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   2  (bases 1 to 153122)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. CcalMP-8W
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:14:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,603
            CDSs (total)                      :: 6,529
            Genes (coding)                    :: 6,424
            CDSs (with protein)               :: 6,424
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 1 (5S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 83 of 105
            Pseudo Genes (internal stop)      :: 5 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:04:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 98536
            Orig. end                         :: 121333
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB890924.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405361
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22797
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="2"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22797
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22797
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12797](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12797
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(861..2249)
                     /locus_tag="B053_RS0112950"
     CDS             complement(861..2249)
                     /EC_number="2.4.1.15"
                     /GO_function="GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101576.1"
                     /locus_tag="B053_RS0112950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="WP_018489026.1"
                     /transl_table=11
                     /translation="MVTEHAEATAQVLVASNRGPITYTLGEDGTLDAKRGGGGLVSGLS
                     AVDDKLWVCAALGEGDREAVRRGVGEPGVRMLDIDAEVHADAYNGIANSVLWFVHHLLY
                     QTPVEPVFDAEFRRQWASYETYNRAFARALAEEAGEGAAVLVQDYHLALVPGMLRELRP
                     DLRIGHFSHTPWAPVDYFRLLPDDIGEQLLRGILGADRAAFLTRRWADAFIGCCTEILG
                     GTGRTRIGVHGLGADADFLRRRSREADVDERMAALREQVGENRKTIVRVDRTELSKNIV
                     RGLHAYRALLETRPEWRERVVHVAFAYPSRQDLAVYRDYTAAVQSLATEINGTYGTGSW
                     TPVVLHVKDDFARSLAAYRLADVALVNPIRDGMNLVAKEVPVVSDHGCALVLSREAGAY
                     EELGEDAIVVNPYDVTGTAEALHEALSMGTEERTGRTKRLAEAATALPPQRWFLDQLEA
                     LRQE"
     gene            complement(2427..3377)
                     /locus_tag="B053_RS0112955"
     CDS             complement(2427..3377)
                     /EC_number="2.4.1.266"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511020.1"
                     /locus_tag="B053_RS0112955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="WP_018489027.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.4e-11,
                     bitscore: 37.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLEEVERWLTRRSWSSADRPLDRLLDARTAEPHRTSVSVVLPALN
                     EEATVGAIVATIRRELMEKVRLVDELVVIDSGSTDATAAVARQAGARVVHRDAVLPRIP
                     ALPGKGEVLWRSLLVTSGEIVCFVDADLKDFSADFVSGTVGPLLTDPTVHFVKAMYDRP
                     FGDTAGQGGRVTELVARPLLNLHWPQLAGFVQPLGGEYAVRRSLLERLPFPVGYGVELG
                     LLVDALHTVGLDALGQVDVGVRRHRHQDGQALGRMAAAIYRTAQLRLSRGHLVRPALTQ
                     FERGAEGFVPRTHAVDTEERPPMREIAEYAERWAA"
     misc_feature    3338..3353
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.07"
                     /tool="antismash"
     misc_feature    3403..3423
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.36"
                     /tool="antismash"
     regulatory      3531..3658
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            3677..4972
                     /gene="thrC"
                     /locus_tag="B053_RS0112960"
     CDS             3677..4972
                     /EC_number="4.2.3.1"
                     /GO_function="GO:0004795 - threonine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thrC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056010.1"
                     /locus_tag="B053_RS0112960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="threonine synthase"
                     /protein_id="WP_026237697.1"
                     /sec_met_domain="PALP (E-value: 1.9e-62, bitscore: 204.5,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVQTIAGNATTASVDLGPAVALSCRECGERFELGPLFACASCFG
                     PLEVAYDLPAGDPEALRKRIEAGPDNIWRYAPLLPVPADVAGKPNLNPGFTKLVKADNL
                     ARELGVTGGLYVKDDSGNPTHSFKDRVVAIAVEAARAFGFTTLSCSSTGNLAGAVGAAA
                     ARAGLRSCVFIPHDLEQGKVVMAAVYGGELVGIEGNYDDVNRFCSELIGDPLGEGWGFV
                     NVNLRPYYGEGSKTLAYEICEQLGWRLPDQIVIPIASGSQLTKIDKGFQELIRLGLVED
                     RPYKIFGAQVEGCSPVSAAFKAGHDVVRPQKPNTIAKSLAIGNPADGPYVLDIARRTGG
                     AVEDVNDEQVVDAIKLLARTEGIFGETAGGVTVGVTKKLIEAGVIDPALTTVVLNTGDG
                     LKTLDAVSATSQATATIKPSLDAFRAAGLASA"
     gene            5026..5304
                     /locus_tag="B053_RS0112965"
     CDS             5026..5304
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265553.1"
                     /locus_tag="B053_RS0112965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MoaD/ThiS family protein"
                     /protein_id="WP_018489029.1"
                     /transl_table=11
                     /translation="MSVKVRIPTILRTYTGGQAEVPAEGATLAEVIESLEKDHPGIAAR
                     VLDDQGKLRRFVNVYVNDDDVRFEGGLDAATPDGAGISIIPAVAGGC"
     gene            5714..5920
                     /locus_tag="B053_RS0112970"
     CDS             5714..5920
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967346.1"
                     /locus_tag="B053_RS0112970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cold-shock protein"
                     /protein_id="WP_003967346.1"
                     /transl_table=11
                     /translation="MAQGTVKWFNAEKGYGFIAVDGGADVFVHYSAIQMDGYRTLEEGQ
                     RVEFEISQGQKGPQADMVKLAVG"
     misc_feature    complement(5762..5775)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    6153..6168
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    complement(6153..6171)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.97"
                     /tool="antismash"
     misc_feature    6155..6169
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.7"
                     /tool="antismash"
     misc_feature    complement(6156..6171)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.7"
                     /tool="antismash"
     gene            6323..7945
                     /gene="groL"
                     /locus_tag="B053_RS0112975"
     CDS             6323..7945
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /gene="groL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /locus_tag="B053_RS0112975"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="chaperonin GroEL"
                     /protein_id="WP_003967345.1"
                     /transl_table=11
                     /translation="MAKIIAFDEEARRGLERGMNQLADAVKVTLGPKGRNVVLEKKWGA
                     PTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLR
                     NVAAGANPMALKRGIEKAVEAVSAALLEQAKDVETKEQIASTASISAADTEIGAKIAEA
                     MDKVGKEGVITVEESQTFGLELELTEGMRFDKGYISAYFATDMERMEASLDDPYILIVN
                     SKIGSVKDLIPLLEKVMQSGKPLLIIAEDVEGEALSTLVVNKIKGTFKSVAVKAPGFGD
                     RRKAMLADIAILTGGTVISEEVGLKLENAGLDLLGRARKVVITKDETTIVDGAGDSDQV
                     QGRVNQIRAEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVR
                     NAKAAVEEGIVAGGGVALLQASAVFEKLDLTGDEATGANAVKLALEAPLKQIAVNGGLE
                     GGVVVEKVRNLPIGHGLNAATGEYVDMIAEGILDPAKVTRSALQNAASIAALFLTTEAV
                     IADKPEKAGAAAPGGMPGGDMDF"
     gene            8346..8939
                     /locus_tag="B053_RS0112980"
     CDS             8346..8939
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762775.1"
                     /locus_tag="B053_RS0112980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_234017963.1"
                     /transl_table=11
                     /translation="MPGSTGPGDIGSGSTGPGNIGGGPRRGERGRWSGRRLKGAALLLA
                     GALLCAVGGFIVVTGTGLSKLFGMRVETFGRIACHDTRAEKGQTVWHCFGETEAQQRAN
                     DAERERVARESLRVHVDGMPASAQIERTRITFADHDGRDDPETITATQAFEGGRWYAHS
                     GQLVVYGMIPLLAGAGTAAWGVYRVREAAGARGR"
     misc_feature    complement(9366..9381)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.15"
                     /tool="antismash"
     misc_feature    9367..9380
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.82"
                     /tool="antismash"
     misc_feature    9536..9551
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.05"
                     /tool="antismash"
     gene            9816..9950
                     /locus_tag="B053_RS1000000136555"
     CDS             9816..9950
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511015.1"
                     /locus_tag="B053_RS1000000136555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_018489031.1"
                     /transl_table=11
                     /translation="MKQQQEKAKKAYMKPSLFKQGDFSKKTAGYFYGSYKEYWVRRII"
     CDS_motif       9816..9899
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKQQQEKAKKAYMKPSLFKQGDFSKKTA"
                     /locus_tag="B053_RS1000000136555_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       9900..9950
                     /prepeptide="core"
                     /locus_tag="B053_RS1000000136555_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="0.20"
                     /molecular_weight="2082.4"
                     /monoisotopic_mass="2081.0"
                     /core_sequence="GYFYGSYKEYWVRRII"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKQQQEKAKKAYMKPSLFKQGDFSKKTA"
                     /leader_location="[9815:9899](+)"
     gene            10001..12076
                     /locus_tag="B053_RS0112990"
     CDS             10001..12076
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010071617.1"
                     /locus_tag="B053_RS0112990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_018489032.1"
                     /sec_met_domain="Asn_synthase (E-value: 5.6e-37, bitscore:
                     121.3, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGLLRDYFVVLPDSVAGHTVAGQLPAPGTTGAASDPTATGPAFA
                     PTATGTSTGTGIEAGTGTGTGTRPGTSTGTGASTGTGDVVTVPHPSGRPWVLARPLFRK
                     VSHLRRGGDALVLIGPGRLPEAVLAGLLEGARDRAGLERRLARLPGLHHVIARISGETW
                     IRGTASGLRRIYHARHPQAGTIASDRPAVLAHLTGAPLDDGALALRLLDFLPHPLSRRA
                     LWRGVHETGAGYGLALPVAAPGTAAAPGPHEYRWWEPPPPELPLAEGARRVGEAIAASV
                     RAHVGGLDRISCELSGGLDSTTLTFAARATAPAELSLLTVAARDRYSEDETWARRAVEA
                     ARGGPTETPAPAHHIIPADQAPYFYADLTATSAELNDEPLPVAPGRARAHLLLSRARAT
                     GSRYHLTGYGGDELFLGLPHAYQDLFHGNPLTAWNHLSGLRHQLGWPLLPTLKALLDRS
                     AFPQWLAGAVTTEPQPVTRTPLLSWGVRQSLRPWFTDHANALITAEFRAAAAEQAEPID
                     RWRGRHVDIDAVRMGARHFQAMEDIGMTIGLPVAAPFYDDRVLEATLAVRLEERISPWR
                     YKPLLVEAMRGVVPDALLARTTKDHMSSDEHQGLREHAHELADLWTGSRLADHGLVDAR
                     HLLRLAAEPFSPVLVEHSISSTVAGETWLRTAEDAWPTLQSTPTATTVTTTSEALK"
     aSDomain        12073..12318
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_B053_RS0112995_Stand_Alone_Lasso_RRE.
                     1"
                     /evalue="1.70E-27"
                     /identifier="RREFam002.1"
                     /label="B053_RS0112995"
                     /locus_tag="B053_RS0112995"
                     /protein_end="82"
                     /protein_start="0"
                     /score="86.3"
                     /tool="antismash"
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSVIVVEALAAGQT
                     PEAAVDRIVAEFDVERTEAESDVAELTRQLVEAKILR"
     gene            12073..12324
                     /locus_tag="B053_RS0112995"
     CDS             12073..12324
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126488.1"
                     /locus_tag="B053_RS0112995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_018489033.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 8.7e-27,
                     bitscore: 86.3, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.4e-15, bitscore: 50.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSVIVVEALAAGQT
                     PEAAVDRIVAEFDVERTEAESDVAELTRQLVEAKILRP"
     gene            12321..12797
                     /locus_tag="B053_RS0113000"
     CDS             12321..12797
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511012.1"
                     /locus_tag="B053_RS0113000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_018489034.1"
                     /sec_met_domain="PF13471 (E-value: 1.6e-26, bitscore: 85.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEMTMPRRGAGPGGVRLRAAIAAAFVLARFRPGRLRRVLTRCS
                     RGARPATYDETLEMYEAVTATSRRCAGRYGCLPRSVAIALACRMSGAWPDWCAGVRTAP
                     PFSPHAWVEAGGRTVGEQAEAADLRPLMVVTVREGVPGERGGGDDDGGGGGQKP"
     gene            13024..14754
                     /locus_tag="B053_RS0113005"
     CDS             13024..14754
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511011.1"
                     /locus_tag="B053_RS0113005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_106669348.1"
                     /transl_table=11
                     /translation="MLLARVRPHRGVLVRAGLLSLAGSAAGLAMPLMAKYAVDAFAADR
                     SPVAPLIALTALVLAGACLSAYGRYLMARTGEGVVLRARDQLVGRIMRLKVPAVDRLTP
                     GDLQSRVTSDTTLLRTVLSSGLVESFNSVLMLLGTVAFMAYMDLTLLGVTLVVIVGIGA
                     VTALLMPRIQRAQLRAQESVGAMGAALDRVLQAFRTVKASGAEERETAAVARAARHAHD
                     RGVSVAKWSSVSDVTMMMSIQLAFLAVLGVGGARVASGSLEISSLIAFLLYLFYLMGPI
                     GGLVEGWTGLQSGLAAVRRIDEVESLPGEPSEGPGGLEEGGGPLRVTFQDVTFGYGDER
                     APAHKGVTFDVPAGGMVALVGPSGAGKSTVFSLLERFYDHDAGTIALDGRDIRDWPLAE
                     LRASLAYVEQDAPVLAGTLRENLLFAAPDAPEERLAEAVARTRLDALVARLPGGLDTAV
                     GHRGVTLSGGERQRIAIARALLRRPRLLLLDEVTSQLDAVNEQALRDVILELAAQSTVL
                     VIAHRLSTVRHADRIVVLEDGRVRTAGTHEELMAKDDLYRELATTQLAAVSEGVEAGAR
                     "
     gene            complement(14759..15346)
                     /locus_tag="B053_RS0113010"
     CDS             complement(14759..15346)
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762767.1"
                     /locus_tag="B053_RS0113010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Uma2 family endonuclease"
                     /protein_id="WP_018489036.1"
                     /transl_table=11
                     /translation="MTPSPADHARRAQLSVEEFEQLARSAPETVTLELINGKLEVKPVP
                     DGDHGTIFMWLLRQCMQHRPDLDLHPEQGLMVEAYRQGRARPDGALAPRRHFAGQGEWA
                     PADGVLMAVEVTSYDHDTDHRDRTEKPHGYASAGIPVYLLIDRDSGTLVVHSEPDRGRY
                     RQQHSYDYGDRVTLPHPVSITLDTEELKDYAR"
     gene            complement(15403..16245)
                     /locus_tag="B053_RS0113015"
     CDS             complement(15403..16245)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511009.1"
                     /locus_tag="B053_RS0113015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018489037.1"
                     /transl_table=11
                     /translation="MAKPGYGKRSAPGERPRTDRDFAHLLPRDAAVAAYLDRLPDGSDI
                     SVKTLAKHLPYGQCALSTTLNRLQAAGHLRRGSECVVADDVMLWVTRTFFSRTAREDSW
                     WNAFTRGDAPPDQPQRPTRSRAFILLAALGRTAPTLSLSSADCVRLEPRVSEWFARGAT
                     EAELLHALTAGLPSPVHCAARLVERRLTDKLPPERVPPGPGPRTVLRTLECGECGVPGS
                     PEALLGGLCRACRGPSPEPPVGPPGAVQPPGVRERMNAVRAGLVPRSERTYRFERTHR"
     misc_feature    complement(16024..16026)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(16425..16441)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.78"
                     /tool="antismash"
     misc_feature    complement(16574..16589)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.55"
                     /tool="antismash"
     gene            complement(16601..17452)
                     /locus_tag="B053_RS0113020"
     CDS             complement(16601..17452)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158402.1"
                     /locus_tag="B053_RS0113020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018489038.1"
                     /transl_table=11
                     /translation="MAKFPDDVDPRQLTVVARRVLIDGLSALQDHLPALTVVGAQAVHL
                     RSPDAAISNAPFTSDGDLSIDPMLLGDQPHLDASLRAAGFDLKYDHEPGLWERPETIGD
                     RVVPVELDLLVPRQLSPKNGRRSAKVPPHGAMSARWIDGLEVAAVDRSPLQVSSLDAED
                     PRSIIVNVAGPTALLVAKAFKITDRLDDADKRPDRLTDKDAGDVLRIMMTASPGRVAAR
                     FAELRKDARVGSTAAEGLEKLHALFGAAATPGVDMAVRALSGDVPEQRVRALAPAFISR
                     LR"
     gene            complement(17493..18635)
                     /locus_tag="B053_RS0113025"
     CDS             complement(17493..18635)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B053_RS0113025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_063826507.1"
                     /transl_table=11
                     /translation="MTAVELTSETLFEQGMARLAELLGPPWQIDRRPDNKETHRSGGDI
                     VVTVQAEGEGSYAQLLVDVKLTGVSPRTVEEVLLAKLDLLRQLNHHTTLLVLTPWLSRQ
                     TQKLLRGHDISYLDLMGNVSLRVPRPAIVIHTEGATRAPQALAKEPGRTTLAGPKAGRL
                     VRLLTDVHPPYRAGELAQASGLSLGYVSRLLDALEDQLLIRRDGRVITSVDWPNLLRAR
                     AARETLLRKGSYVGLLAPNGVPAVIERIRHLPQSGLDNIAVTGTYAAHTVAPLAAGGQL
                     MVYTSPILDEDDLGEELGLLPVDENADVLLLQARDRSVFERTRIVDGIPHVALSQAALD
                     CLAGPGRLPAEGEAVIEYMKAHQRRWQVSGMENLLTPDQL"
     misc_feature    18875..18892
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            18942..19442
                     /locus_tag="B053_RS0113030"
     CDS             18942..19442
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489040.1"
                     /locus_tag="B053_RS0113030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_018489040.1"
                     /transl_table=11
                     /translation="MTVEEFEEFYAQAVARLTGQLYVMTGDLQEAQDVVQEAFVKAWVR
                     RGRLERDGQPEAWIRTVAWRLAVSRWRFRRRSADAWDRGSGAPEHVAPPDPDHVVLVEA
                     LRGLPAQQRRTLTLHYLCDLTVEQIAGETGLSTSTIKTHLVRGRAALAHRLRDPRMEEA
                     PDV"
     misc_feature    complement(18957..18970)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.98"
                     /tool="antismash"
     gene            19435..19968
                     /locus_tag="B053_RS1000000137160"
     CDS             19435..19968
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126494.1"
                     /locus_tag="B053_RS1000000137160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_078507678.1"
                     /transl_table=11
                     /translation="MSEPRDPSGPEEPRGAQDPLRTLFHQAGEFGQGRAVADPAAHITE
                     RGMRAQRRRMAALAVGVCLVVGGGGAVAVGLHPVTPTPSPPATSPSPSYPSPSPPPPAG
                     TPTEPPTPSTTMPGAQGTGPGAYSSGSYTPGSSVPGSSKPGGMPTAEAPTAPSTSTGPP
                     GSTPTSTLGSTSMP"
     gene            20127..21443
                     /locus_tag="B053_RS0113040"
     CDS             20127..21443
                     /GO_function="GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010055996.1"
                     /locus_tag="B053_RS0113040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mannosyltransferase family protein"
                     /protein_id="WP_234017964.1"
                     /transl_table=11
                     /translation="MPISSPGLRPAEPRSGSTSAPRSGATSAPRANRPVRPVPYASESP
                     RRDRWASWLTPADRRVLQLYLLTRLSLWTMAHGARWLFPRGSDVREAAPLLAPFQQWDA
                     NHYLHIARDGYFPAGAGPWLGDWDNREAFFPGYPLLLRAVHVVIPDWTAAGLLISLVAG
                     AVAVLALSRTARSYVPEGGAGHRTVLFFLLSPCAVFLAAGYTEALFLAFALPAWLAARR
                     HQWAWACALTALATTVRVSGLFLAAAIAVLFVLTALQAGSGKRKGKGRRTAWRGVAWLV
                     LPGLPPLAYSWYLHAHTGDWMGWKHAQERGWYREFLTPWEAWANTWNAAFGGVHTTGYA
                     AMFQAELAAMVVGVALVALLARYGRWPEAVYVALSLWALGTSYWYTSIPRATLLWWPLW
                     IGLAAVSLRRPWFRTAYLCVSAPLAALVALAFFTGRWAG"
     gene            complement(21457..22737)
                     /locus_tag="B053_RS0113045"
     CDS             complement(21457..22737)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038: phenylalanine-specific permease"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015580428.1"
                     /locus_tag="B053_RS0113045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="APC family permease"
                     /protein_id="WP_018489043.1"
                     /transl_table=11
                     /translation="MVDVSGAPAGARGMERRLGLSDAVVIGLGSMVGAGIFAALGPAAD
                     AAGSGLLLALGLASVVAYCNATSSARLAALYPESGGTYVYGRERLGEFWGYLAGWAFVV
                     GKTASCAAMALTVGAYLWPGQAHAVAVAAVVALTAVNYAGVQKSVFLTRIVVCTVLAVL
                     AAVVVTALGSAEADAARLGIGPDVTMGGVLQAAGLLFFAFAGYARIATLGEEVRDPART
                     IPRAIPVALAITLAVYGLVAVAVLAALGPHGLAAATAPLSDAARAAGADWLVPVVRVGG
                     AVAALGALLGLILGVSRTTLAMARDRHLPHALAAVHPRFGVPHRAELAVGTVVVVLAAT
                     ADVREVIGFSSFGVLAYYAIANASAWTLSAEEGRPNRLIPLTGLAGCTALAFALPPGSV
                     VTGAAVLVAGGVVHGLQRAISRSCKEN"
ORIGIN
        1 cgacggcacg ctcgccgaca tcgtccccga ccccgagcag gcccgggccc accccggcgc
       61 cgccgaggcg ctcgccgcgc tcgccccgaa ggtcgcctcg atcgccgtga tcaccggccg
      121 cccggcaggc gtggccgtac ggtacggcgg cttcgcgggc gtccccggcc tggaccacct
      181 cgtcgtcctc ggccactacg gcgccgagcg ctgggacgcc gccaccggca ccgtgaacgc
      241 tcccgccccg caccccggcg tggccgaggc gcgggccgaa ctccctggcg tactgcacga
      301 gttcggtgcc tggcacggca cctggatcga ggagaagggc caggccgtcg ccgtccacac
      361 ccgccgtgcc gacgacccgc aggcggtctt cgagaccctg cgcggccccc tcggcgagct
      421 ggccgcccgc cacgggctga tcctcgaacc gggccgccag gtcctggagt tgcgcccgcc
      481 gggcatggac aagggcgtcg cgctcgcgga gtacgtacgg gaggtgggcg ccacctccgt
      541 gctgtacgcg ggcgacgacc tcggggacct cgccgccttc gccacggtgg agaagctgcg
      601 cggcgcgggc acggagggga tcccgggact gctggtgtgc agcggcagcg ccgaggtgcc
      661 cgaactggcc gagcgcgcgg acctgttggt gccgggaccg gccgccgtgg tggagctcct
      721 ggcggcgctg gccgaccgcc tctgagccgc gctcgcacac acctggcccg aacccctccg
      781 agccgtaccg gaggtgccgg gagtaccgga agcgccgggc gtacggagct gagcgccggg
      841 agtgccgcga gcggctcctc tcactcctgc cgcagcgcct ccagctgatc caggaaccat
      901 cgctgcggcg ggagcgccgt cgccgcctcc gccagccgct tcgtgcggcc cgtccgctcc
      961 tcggtgccca tcgacagggc ctcgtgcagc gcctccgccg tgcccgtcac gtcgtacggg
     1021 ttcaccacga tcgcgtcctc gcccagctcc tcgtacgccc ccgcctcccg tgacagcacc
     1081 agcgcgcagc cgtggtcgga gacgacgggg acctccttgg cgaccaggtt catgccgtcg
     1141 cggatcgggt tgaccagcgc cacgtccgcc agccggtacg cggcgagcga gcgggcgaag
     1201 tcgtccttca cgtggaggac gacgggcgtc cagctcccgg tcccgtacgt cccgttgatc
     1261 tccgtggcca gcgactgcac cgccgccgtg tagtcccggt agaccgcgag gtcctggcgg
     1321 gaggggtagg cgaaggccac gtggaccacg cgctcgcgcc actccgggcg ggtctccagc
     1381 agggcccggt aggcgtgcag gccgcgcacg atgttcttgg acagctccgt gcggtccacc
     1441 cgcacgatcg tcttccggtt ctcgcccacc tgctcccgca gcgcggccat ccgctcgtcc
     1501 acgtccgcct cgcgcgagcg ccggcgcagg aagtccgcgt ccgcccccag cccgtgcacc
     1561 ccgatccggg tccggcccgt cccgccgagg atctccgtgc agcagccgat gaacgcgtcc
     1621 gcccaccggc gggtcaggaa cgcggcccgg tccgcgccga ggatgccccg cagcagctgc
     1681 tcaccgatgt cgtcgggcag cagccggaag tagtccaccg gcgcccacgg ggtgtgcgag
     1741 aagtggccga tccgcaggtc ggggcgcagc tcgcggagca tcccggggac cagggccagg
     1801 tggtagtcct ggaccagcac cgccgcccct tcccccgcct cctccgcgag ggcgcgggcg
     1861 aaggcccggt tgtacgtctc gtaggaggcc cactgccgcc ggaactccgc gtcgaagacg
     1921 ggctccacgg gggtctggta cagcaggtgg tggacgaacc acagcaccga gttggcgatg
     1981 ccgttgtacg cgtcggcgtg cacctcggcg tcgatgtcca gcatccgtac gcccggctca
     2041 ccgaccccgc gccgcaccgc ctcgcggtcg ccctccccga gggccgcgca gacccacagc
     2101 ttgtcgtcga cggcgctcag cccggagacg agccccccgc cgccgcgctt ggcgtcgagc
     2161 gttccgtcct cgccgagcgt gtacgtgatg gggccgcggt tggacgcgac gaggacctgg
     2221 gcggtggctt cggcatgctc ggtgaccatg tagcggaacc tagcccgtaa gggaagccgc
     2281 caaacgcgcg tccggcggac cggtcaccgg caccgcgccg cccaccgcgc ccgcgtacgc
     2341 cgcacgccgg ccgcctcgcc cgcgcacggc tcagggctgc cgcagctccg cgcccgcccc
     2401 ggagctgccg gagccccgcg cacgcctcac gccgcccacc gctccgcgta ctccgcgatc
     2461 tcccgcatgg gcggccgctc ctccgtgtcc accgcatggg tgcgcggcac gaacccctcc
     2521 gccccccgct cgaactgggt cagcgcgggc cgcaccagat ggccccggga gagccgcagc
     2581 tgggccgtgc ggtagatcgc cgccgccatc cggcccagcg cctgcccgtc ctggtggcgg
     2641 tggcgccgca cccccacgtc gacctggccc agcgcgtcga ggcccacggt gtgcagcgcg
     2701 tcgaccagga gccccagctc cactccgtaa ccgaccggga acggcagccg ctccagcagc
     2761 gagcgccgta ccgcgtactc accgcccagc ggctgcacga acccggccag ctgcggccag
     2821 tgcaggttga gcagcgggcg ggccaccagc tcggtgacgc gaccgccctg acctgcggtg
     2881 tcgccgaacg gacggtcgta catcgccttg acgaagtgca ccgtgggatc ggtgagcagc
     2941 gggccgaccg tccccgagac gaagtccgcc gagaagtcct tgaggtccgc gtcgacgaaa
     3001 cagacgatct ccccgctggt caccaggagc gaccgccaga gcacctcgcc cttgcccggc
     3061 agggccggta tccggggcag caccgcgtcc cggtggacca cccgcgcgcc cgcctggcgg
     3121 gcgacggcgg cggtcgcgtc ggtcgaaccg gagtcgatca ccaccagttc gtccaccagc
     3181 cggaccttct ccatcagctc ccgccggatc gtcgccacga tcgccccgac cgtggcctcc
     3241 tcgttcagcg cgggcagcac cacactcacc gaggtgcggt gcggttcggc ggtccgggcg
     3301 tcgaggagcc ggtccagcgg gcggtcggcg gacgaccagg accgcctggt cagccagcgt
     3361 tccacctctt cgagcacggt ctgaactccc tgtgtgtgat ccatctcgcc gttcggacga
     3421 ctatctcaac cgtcccgtcc ttcggttaca gtcttgaaca acgcggacga ccgtcgcatg
     3481 tcggggtccg tcgtcacaca aacgcccgga tcgaagatcc ggtgccacag agctcatcca
     3541 gagggactga gggaacggcc cgttgaagtc ccggcaaccc tcctgccgac cgcgaggtca
     3601 cggtggggaa ggtgccaatt ccgtcttgcg gcgaaatgcg ctgcgaggaa gatgaggaga
     3661 aagggcctcc gccatcatgg ctgttcagac catcgcaggt aatgccacca ccgcttccgt
     3721 ggacctcggt cccgccgtgg cgctttcctg ccgcgagtgc ggcgagcgtt tcgagctcgg
     3781 cccccttttc gcctgtgcgt cctgtttcgg gccactggaa gtggcgtacg acctgccggc
     3841 cggtgacccg gaggccctgc gcaagcgcat cgaggccggt cccgacaaca tctggcgcta
     3901 cgccccgctg ctgccggtcc ccgccgacgt ggccggcaag cccaacctga accccggctt
     3961 caccaagctg gtcaaggccg acaacctcgc ccgcgagctg ggcgtcaccg gcggtctgta
     4021 cgtcaaggac gactccggca acccgacgca ctccttcaag gaccgcgtcg tcgcgatcgc
     4081 cgtcgaggcc gcccgcgcct tcggtttcac caccctctcc tgctcctcca cgggcaacct
     4141 ggccggtgcc gtcggcgccg ccgccgcccg cgccggactg cgctcctgcg tcttcatccc
     4201 gcacgacctg gagcagggca aggtcgtcat ggccgcggtg tacggcggtg agctggtcgg
     4261 catcgagggc aactacgacg acgtgaaccg cttctgctcc gagctgatcg gggacccgct
     4321 cggcgagggc tggggcttcg tcaacgtcaa cctgcggccc tactacggcg agggctccaa
     4381 gaccctggcg tacgagatct gcgagcagct cggctggcgg ctgcccgacc agatcgtcat
     4441 cccgatcgcg tccggctccc agctgacgaa gatcgacaag gggttccagg agctgatcag
     4501 gctcggcctg gtcgaggacc ggccgtacaa gatcttcggc gcccaggtcg agggctgctc
     4561 cccggtctcc gccgccttca aggccgggca cgacgtggtc cgcccccaga agccgaacac
     4621 catcgccaag tccctggcca tcggcaaccc ggcggacggc ccgtacgtcc tggacatcgc
     4681 ccgccgcacg ggcggcgcgg tggaggacgt gaacgacgag caggtcgtcg acgcgatcaa
     4741 gctgctggcg cgcaccgagg gcatcttcgg cgagaccgcg ggcggtgtca cggtcggcgt
     4801 gacgaagaag ctgatcgagg ccggggtgat cgacccggcg ctcaccaccg tcgtcctgaa
     4861 caccggtgac gggctcaaga cgctcgacgc ggtctccgcg acctcgcagg cgacggccac
     4921 catcaagccg agcctggacg cgttccgcgc cgcgggcctc gcctccgcct gatcatcccg
     4981 atcgccccga tcggcccgac cgcagcagtc agaaaggcgt ccgtcatgag cgtcaaggtc
     5041 cgcatcccca ccatcctccg cacctacacg ggcggccagg ccgaggtccc ggccgagggc
     5101 gccacgctcg ccgaggtcat cgagtccctg gagaaggacc accccggcat cgccgcccgc
     5161 gtcctggacg accagggcaa gctgcgccgc ttcgtgaacg tgtacgtcaa cgacgacgac
     5221 gtgcgcttcg agggcggcct ggacgcggcc acgccggacg gcgccggtat ctcgatcatc
     5281 cctgccgtgg ccggcggctg ctgaacccgt acgaacacca ttgccccctc cgcccctcgg
     5341 ggaggagggg gcaattctgc atggttgagc acggtacagt tggggaaacc cccttcgctg
     5401 cacgcgccgc ccgcatatga gaatgcgccc ggacccgata agaagcagcc aaagtgcgtg
     5461 aaccccttga ggcataagtc acctttgcct ggcccgactt gcccgtaaat ctcagcaatc
     5521 ccgcatattc gggcgttcgg ccgtgcccgg aattctcgtc cgattgacct gttgcagagg
     5581 gcagttgggc ggatacattc ggccgcggtc gacgcgctgc ggcgctgcgc accctctcct
     5641 gtcggagggt gagttccgac ccggggccgc gaagtgcggt cccgtgcaag ggccagttat
     5701 aggggagtta ggcatggctc agggcaccgt caagtggttc aacgcggaga aggggtacgg
     5761 cttcatcgcg gtcgacggtg gtgcggatgt tttcgtccac tacagcgcga tccagatgga
     5821 cgggtaccgc accctcgaag agggtcagcg agttgaattc gagatctcgc agggccagaa
     5881 ggggccccag gcagacatgg tcaagctcgc cgtcggctga gcccgacacg gccgtccgcc
     5941 gtcactactc acgcacgtag ggcccgaccc cggcatccgg ggatcgggcc ctacgcgcgt
     6001 gccggggccg atccgcgggc gcgcccggac cggcccctgg ggatgcccga accggcctgc
     6061 gaggacgccc ggaccggtcc cggatcgggc ggggaccgac ccgggaccgc atggcgggct
     6121 ctcgtcgaag gcgcttgcac tctcgggggt cgagtgctaa tcattggcat tagcactctc
     6181 caggtgagag tgacagaact tggaccgggc cggtgaggcc cgcaggcgtg ttggggcaag
     6241 gaaccaccac gcacgcaggc cgtccgtcgc gggcgcctct cggtccggag caatccaccc
     6301 ctgtccggga ggaccacttc acatggccaa gatcatcgcg ttcgacgagg aggcacggcg
     6361 cggtctcgag cgcgggatga accagctcgc cgacgccgtc aaggtcaccc tcggccccaa
     6421 gggccgtaac gtcgtcctcg agaagaagtg gggcgcgccc acgatcacca acgatggtgt
     6481 atccatcgcc aaggagatcg agctcgagga cccgtacgag aagatcggtg cggagctggt
     6541 caaggaggtc gccaagaaga cggacgacgt cgccggcgac ggtacgacca ccgccaccgt
     6601 cctcgcccag gctctcgtcc gcgagggtct gcgcaacgtc gccgcgggcg ccaacccgat
     6661 ggccctcaag cgcggtatcg agaaggccgt cgaggccgtc tccgccgctc tgctggagca
     6721 ggccaaggac gtggagacca aggagcagat cgcttcgacc gcctccatct ccgccgccga
     6781 caccgagatc ggcgcgaaga tcgccgaggc gatggacaag gtcggcaagg aaggcgtcat
     6841 caccgtcgag gagtcccaga ccttcggtct ggagctggag ctcaccgagg gtatgcgctt
     6901 cgacaagggc tacatctcgg cctacttcgc gaccgacatg gagcgtatgg aggcgtcgct
     6961 cgacgacccg tacatcctga tcgtcaactc gaagatcggc agcgtcaagg acctcatccc
     7021 gctgctggag aaggtcatgc agtcgggcaa gccgctgctg atcatcgccg aggacgtcga
     7081 gggcgaggcg ctctccaccc tggtcgtcaa caagatcaag ggcacgttca agtccgtcgc
     7141 cgtcaaggcc ccgggcttcg gcgaccgccg caaggccatg ctcgcggaca tcgccatcct
     7201 caccggcggc accgtgatct ccgaggaggt cggcctcaag ctggagaacg ccggtctcga
     7261 cctgctcggc cgcgcccgca aggtcgtcat caccaaggac gagacgacca tcgtcgacgg
     7321 tgccggtgac agcgaccagg tccagggccg cgtcaaccag atccgcgccg agatcgagaa
     7381 ctccgactcg gactacgacc gcgagaagct ccaggagcgc ctcgcgaagc tggccggcgg
     7441 cgtggccgtc atcaaggccg gcgccgccac cgaggtggag ctcaaggagc gcaagcaccg
     7501 catcgaggac gcggtgcgca acgccaaggc cgccgtcgag gagggcatcg tcgccggtgg
     7561 tggcgtggcc ctgctccagg cctcggccgt cttcgagaag ctcgacctca cgggcgacga
     7621 ggcgaccggc gccaacgccg tgaagctcgc gctggaggcc ccgctcaagc agatcgccgt
     7681 caacggtggt ctcgagggtg gcgtcgtcgt ggagaaggtc cgcaacctgc cgatcggtca
     7741 cggcctcaac gccgcgacgg gtgagtacgt cgacatgatc gccgagggca ttctcgaccc
     7801 ggcgaaggtc acgcgctccg ccctgcagaa cgccgcctcc atcgccgcgc tcttcctcac
     7861 caccgaggcc gtcatcgccg acaagcccga gaaggccggc gcggccgccc cgggtggcat
     7921 gccgggcggt gacatggact tctgatcggt tccggtccgc cggtcgatcg aggttccgcg
     7981 tacgacgggt acgtcaggtg cgccgagtac gcaggtacgt cccacagggg cggcatctcc
     8041 cagccgggag gtgccgcccc tgtggcgtgt ccggggcagc aggggagagt acggagccgg
     8101 gccgtacggg aaccgtcagc gcgtgggaac ggtccaacgg ttcatacggg ctgacggttc
     8161 acgcacgctg gcaacggctc acgcatgctg gcaacggtgg atacgcggga acggttgacg
     8221 cgcgcggggg acgcgtgcgg cacgcgaacg ggggcggggc ggtcatgacg gccatgggca
     8281 acaccgggtc gggcaacacc gggtcgggga acatcgggtc gggtagcacc gggcccggga
     8341 acaccgtgcc gggtagcacc gggccgggcg acatcgggtc gggtagcacc gggccgggga
     8401 acatcggcgg cgggccgcgg cgcggggagc gcgggcggtg gagcgggcgg cggctcaagg
     8461 gcgccgcgct gcttctcgcg ggggcgctcc tgtgcgcggt gggcggcttc atcgtggtca
     8521 ccggcacggg cctctcgaag ctgttcggca tgcgggtgga gaccttcggc cggatcgcgt
     8581 gccacgacac gcgcgccgag aagggtcaga ccgtctggca ctgcttcggg gagaccgagg
     8641 cccagcagcg cgccaacgac gcggagcgcg agagggtcgc ccgggagtcc ctgcgcgtcc
     8701 atgtcgacgg gatgcccgcg tcggcgcaga tcgagcggac ccggatcacc ttcgcggacc
     8761 acgacggccg agacgacccc gagacgatca ccgcgaccca ggccttcgag ggcggccggt
     8821 ggtacgcgca ctcgggccag ctcgtcgtct acgggatgat ccccctcctc gcgggcgccg
     8881 gaacggcggc gtggggcgtc taccgggtac gcgaggcggc gggcgcgcgg ggccgctgag
     8941 ggctgatgag gaccgctgcg agcgccttgg cggacgcccc ggcggatgcc ccggcggact
     9001 cagggcccct tatgggtcgc ccaaagcgcg ctcggggtgg gcccaaggcg cgctttgggt
     9061 gggcccgaag gcgggcccag ggccgcccgc tcacggtgca ctcgcggcgc gctcgcgggg
     9121 cgcgggcggg gcgggctggt ggggcgcggg cgggctggtg ggcgttcggc gtgccctggc
     9181 ggggctcggg taggcggctg cgggatgcag gcggggctcg ggcgggcggc cgcgggatgc
     9241 agacggggct cggacgggcg caggtgggct cggaggggac ctcggcgggc ccgagcggcc
     9301 ccggacggaa ccgaacgagc ggtcgggagt ggccgaatgg ctacttgccg tgcccggcac
     9361 cgtacggcaa cacacgttcg agcggtttcg tcgatgccgg tggcgcgccg gccgtgcgtg
     9421 aggtaaggcc ggtatcggcg ctgccaggcc cccgccccgc tggttcctcc gtccggccgc
     9481 gccccgcgcg atttcttggc tgctcactgc cggggtcggg ccgccgcccg tagccattgg
     9541 tccagtccat tgcgccggac cggcccgtcg ctgtgcgact gcacatgccg accggtcggt
     9601 gcctcggcgg cggtaggggt cgtcgcgacc gcactctctc cgctcgcgct atccggccaa
     9661 cttcgcgcgg taagtcggaa tcacgcgtac cggtccggca gcgggacatg atggccctgg
     9721 tgatcacctg gtgaacacca ggcgcaccaa taactccagt tatgcgaaca cctgttgggc
     9781 ataacggtcg tcaaccgcac agaagggaag gtgtcatgaa gcagcagcag gagaaggcca
     9841 agaaggccta catgaagccg tcgctgttca agcagggcga cttttcgaag aagaccgccg
     9901 gttacttcta cggctcgtac aaggagtact gggtccggcg catcatctga accgccgacc
     9961 ggtttcctgt gcacggtgtg agaacttccg aaaggtgaag atgcccggcc tgctgcgtga
    10021 ctacttcgtc gtcctgccgg acagcgtggc gggccacacg gtggccgggc agcttcccgc
    10081 accggggacg accggggcgg cgtcggaccc gaccgcgacc gggcccgcgt tcgccccgac
    10141 agcgaccggc accagcaccg ggaccgggat cgaggccggg accggcaccg ggaccggcac
    10201 cagacccggg accagcaccg gcaccggcgc cagcaccggg accggagacg tcgtgaccgt
    10261 cccgcatccc tccggccgcc cctgggtcct cgcgcgcccc ctgttccgca aggtcagcca
    10321 cctccgccga ggcggtgacg ccctggtgct catcggcccc ggccgcctcc ccgaggccgt
    10381 gctcgccggc ctcctggagg gcgcccgcga ccgggccggg ctggagcggc gcctcgcccg
    10441 gctccccggc ctccaccacg tcatcgcccg gatctccggc gagacctgga tccggggcac
    10501 cgcctccggg ctgcgccgga tctaccacgc ccggcacccg caggccggga ccatcgcctc
    10561 ggaccgcccg gccgtcctgg cccacctgac cggcgccccc ctggacgacg gcgcgctggc
    10621 cctgcgcctg ctcgacttcc tgccgcaccc cctgagccgg cgcgccctgt ggcggggcgt
    10681 gcacgagacc ggggccgggt acggactggc cctgcccgtc gcagcaccgg gcacggccgc
    10741 cgcccccggc cctcacgagt accgctggtg ggagcccccg ccgccggaac tccccctggc
    10801 ggagggcgcc cgcagggtcg gggaggccat cgccgcctcc gtacgggccc atgtcggcgg
    10861 cctggaccgg atcagctgcg aactctccgg cggcctggac tccacgaccc tcaccttcgc
    10921 cgcccgcgcg accgccccgg cggagctgtc actcctcacc gtggcggccc gggaccgcta
    10981 cagcgaggac gagacgtggg cgcggcgggc ggtggaggcg gcacgtggcg ggcccacgga
    11041 aacccccgcc ccggcccacc acatcatccc ggccgaccaa gccccgtact tctacgcgga
    11101 cttgaccgcc acctcggccg agctgaacga cgaaccgctc cccgtcgccc ccggccgcgc
    11161 ccgcgcgcac ctgctcctga gccgcgcccg cgccaccgga tcgcgctacc acctcaccgg
    11221 ctacggcggc gacgaactct tcctcggcct gccccacgcc taccaggacc tcttccacgg
    11281 caaccccctc accgcctgga accacctcag cggcctgcgc caccagctcg gctggcccct
    11341 gctccccacc ctcaaggccc tgctggaccg ctccgccttc ccccagtggc tggcgggcgc
    11401 ggtcaccacc gaaccgcagc cggtcacccg gaccccgctg ctctcctggg gcgtacggca
    11461 gtccctgcgc ccgtggttca cggaccacgc gaacgccctg atcactgcgg agttccgggc
    11521 ggccgccgcc gagcaggccg agccgatcga ccggtggcgg gggcggcatg tggacatcga
    11581 cgcggtacgc atgggggcgc ggcacttcca ggcgatggag gacatcggca tgacgatcgg
    11641 gctgcccgtc gcggccccct tctacgacga ccgggttctg gaagccaccc tcgcggtacg
    11701 cctggaggag cggatcagcc cctggcgcta caagccgctg ctcgtcgagg cgatgcgcgg
    11761 ggtcgtgccg gacgcgctgc tggcccgtac gacgaaggac cacatgtcct ccgacgaaca
    11821 ccagggactc cgcgaacacg cccacgaact cgcggacttg tggaccggat cccgcctggc
    11881 cgaccacgga ctggtggacg cccgccacct gctccggctg gcggccgaac ccttctcgcc
    11941 ggtcctggtc gagcactcca tcagctccac cgtggccggg gagacctggc tccgcacggc
    12001 ggaagacgcc tggcccaccc tccagtcgac ccccacggcc acgaccgtga ccaccaccag
    12061 cgaggcactg aagtgaagct cagaaaaggc atcgccgtca cgacgaccga gtacggcgga
    12121 gtcctgctcg acgagaagga cggcagctac tggcagttga acgacaccag cgtcatcgtg
    12181 gtcgaagctc tcgccgccgg gcagaccccc gaggcggccg tcgaccggat cgtcgcggag
    12241 ttcgacgtcg agcggaccga ggcggaatcg gacgtggccg agctgacccg ccagctcgtc
    12301 gaggcgaaga tcctgcgccc atgaccaccg agatgaccat gccccgccgg ggcgcgggcc
    12361 cgggaggcgt gcggctgcgg gccgccatcg ccgccgcgtt cgtcctcgcc cgcttcagac
    12421 cgggccgcct gcgacgggtg ttgacccgct gcagcagggg cgcgcgcccg gcgacgtacg
    12481 acgagacgct ggagatgtac gaggccgtca ccgccaccag ccgccgctgc gcgggccgtt
    12541 acggctgcct gccccgctcg gtggccatcg cgctggcgtg ccgcatgtcg ggggcgtggc
    12601 ccgactggtg cgccggggtc cggacggcac cgccgttctc cccgcatgct tgggtggagg
    12661 cggggggccg cacggtcggc gagcaggcgg aagcggccga tctgcgtcct ctgatggtgg
    12721 tgacggtacg cgagggggtt ccgggtgagc gcggtggcgg ggacgacgac gggggcgggg
    12781 gcggacagaa gccctgaggg gcagccggaa gggcgtgggg cgcctgggcg gccggaaggg
    12841 cctgcggcgc ctgaggcggg cgcacctctc tccgcagcga gcgacccggg cgactccggc
    12901 gcgaccggtg ccgccagcgc ggcctgcgct acgggcacga ccggcgccac cagcacggcc
    12961 ggcgccacca gcacggtcgg cgcgaccggc gccaccggca catccggccc ctcccccctc
    13021 cgtctgctcc tcgcccgggt ccgcccgcac cggggcgtcc tggtccgggc gggcctgctc
    13081 tcgctcgccg ggtcggcggc cgggctggcg atgccgctga tggcgaagta cgcggtcgac
    13141 gcgttcgccg ccgaccgctc gcccgtggcc ccgctcatcg ccctcaccgc gctcgtcctc
    13201 gcgggcgcgt gcctctccgc gtacgggcgt tacctcatgg cccgtacggg agaaggcgtc
    13261 gtgctccgtg cgcgcgacca actggtcggc cgcatcatgc ggttgaaggt cccggcggtc
    13321 gaccgtctca cccccggcga cctccagtcc cgggtgacca gcgacaccac cctcctgcgg
    13381 acggtcctct ccagcggcct ggtcgagtcg ttcaacagtg tgctgatgct gctggggacg
    13441 gtcgcgttca tggcgtacat ggacctcacg ctgctgggcg tgacgctggt ggtgatcgtc
    13501 ggtatcggag ccgtgaccgc cttgctcatg ccgcgcatcc agcgcgcgca gctccgggcg
    13561 caggagtccg tgggggcgat gggcgcggcc ctggaccggg tgctccaggc gttccgtacg
    13621 gtgaaggcga gcggcgctga ggagcgggag acggcggcgg tcgcgcgggc ggcccggcac
    13681 gcgcacgacc ggggtgtgtc ggtggcgaag tggtcgtcgg tctccgacgt cacgatgatg
    13741 atgtcgatcc aactcgcctt cctggccgtg ctgggcgtgg gcggggcgcg ggtggcctcg
    13801 ggctccctgg agatctcctc gctgatcgcg ttcctgctct acctcttcta cctgatgggc
    13861 ccgatcggcg gtctggtgga ggggtggacg gggttgcaga gcggtctcgc cgccgtacgg
    13921 aggatcgacg aggtggagtc gctgccgggc gaaccgtcgg agggccccgg cgggttggag
    13981 gagggcggcg gcccgctgcg ggtcaccttc caggacgtca ccttcggata cggggacgag
    14041 cgtgcccccg cccacaaggg cgtcacgttc gacgtcccgg ccggcgggat ggtggcgctg
    14101 gtgggcccgt cgggggcggg caagtcgacg gtgttcagcc tgctggaacg gttctacgac
    14161 cacgacgccg ggaccatcgc gctggacggc cgggacatca gggactggcc cctcgcggag
    14221 ctgcgcgcct cgctcgcgta cgtggaacag gacgccccgg tcctggcggg caccctccgc
    14281 gagaacctgc tcttcgcggc ccccgacgcc cccgaggagc ggctggcgga ggcggtcgcc
    14341 cgcacccgcc tggacgccct ggtcgcccgg ctgcccgggg gcctggacac ggcggtcggc
    14401 caccggggcg tcaccctctc cggcggcgaa cgccagcgca tcgccatcgc ccgcgccctg
    14461 ctgcgccgcc cgcgcctgct cctcctggac gaggtgacct cgcagctcga cgcggtcaac
    14521 gaacaggcac tgcgcgacgt catcctcgaa ctggccgcac agtcgacagt gttggtcatc
    14581 gcccaccgcc tctccaccgt ccgccacgcc gatcgcatcg tggtcctgga ggacggccgc
    14641 gtccgcacgg cgggcacgca cgaggagctg atggccaagg acgacctcta ccgcgaactg
    14701 gcgacgacgc agctcgcggc ggtgtcggaa ggagtggagg cgggggcgcg gtgaggggtc
    14761 agcgggcgta gtccttgagc tcctcggtgt ccagggtgat ggagacgggg tggggaagcg
    14821 tcacccggtc cccgtagtcg tacgagtgct gctgccggta gcgacctcgg tcgggctcgc
    14881 tgtggacgac gagggtgccg gagtcgcggt cgatcaggag gtagaccggg atgcccgccg
    14941 aggcgtatcc gtggggcttc tccgtgcggt cgcggtgatc ggtgtcgtgg tcgtaggagg
    15001 tcacttccac ggccatcagg acgccgtcgg cgggtgccca ctcgccctgt ccggcgaagt
    15061 gccgacgagg agccagggca ccgtcgggac gggcccgccc ctgccggtac gcctcgacca
    15121 tcagcccttg ctccggatgc aggtccaggt cgggccgatg ctgcatgcac tggcggagca
    15181 gccacatgaa gatggttccg tggtcgccgt cgggcacggg cttgacctct agctttccgt
    15241 tgatgagctc aagcgtcacg gtctccgggg ccgagcgggc gagctgctcg aactcctcga
    15301 cggacagctg ggcccgtcgt gcgtgatcgg cggggctggg ggtcatggcg tcgcctcctc
    15361 ccctccatcg tgccctgccg aatctgcgga tgagctgcac ggtcaccggt gggtcctctc
    15421 gaatcggtag gtccgctccg agcgggggac cagacccgcc cggaccgcgt tcatccgctc
    15481 ccgtacgccg ggcggctgca cggcccccgg cggacccacc ggcggctcgg gggacggccc
    15541 ccggcaggcg cggcacagcc cgccgagaag ggcctccggg ctgccgggga ccccgcactc
    15601 gccgcactcc agggtgcgca ggacggtccg tggccccggc cccggcggga cgcgctcggg
    15661 cggcagcttg tcggtcaggc ggcgttcgac gaggcgggcg gcgcagtgga cgggggacgg
    15721 caggcccgcg gtgagggcgt gcagcagctc ggcctcggtc gcaccccgcg cgaaccactc
    15781 gctgacccgc ggttccagcc gtacgcagtc ggcggaggag agggagagcg tgggggccgt
    15841 acgccccagg gcggcgagca ggatgaacgc ccgcgatcgg gtggggcgtt ggggctggtc
    15901 gggcggggcg tcgccccggg tgaaggcgtt ccaccaggag tcctcgcggg cggtgcgcga
    15961 gaagaacgta cgggtcaccc agagcatcac gtcgtcggcc accacgcact cgctgccgcg
    16021 ccgtaagtgc cccgcggcct ggagccggtt gagggtggtg ctcagggcgc actgcccgta
    16081 cgggaggtgc ttggcgagcg tcttcacgga gatgtcggag ccgtcgggca gccggtcgag
    16141 gtaggcggcg acggcggcgt cgcgggggag gaggtgggcg aagtcgcggt cggtacgcgg
    16201 tcgttcgccg ggggcggagc gcttgccgta gccgggtttg gccatggggt gcgggggcgc
    16261 ggtctcatgc gaggtgctgt gggcagcact aaggtagctg tcagccatgg ggatcgttct
    16321 gtccgatctt cactggtcag acccctgttc ggtgttccag caccagcggg ggtcgtcttt
    16381 gttctgctct cgttctccgc acgctaaagc cacgcgacgg cgcgtcgcaa gtcggtcgca
    16441 aacggtcaac ttcggtcgca gggtgggtgg gtgggacaac tgacccctcc accccatcac
    16501 tgttaagagc gctcggatct cgccgcttga gctccgggag gggcggggtg gagtgacggc
    16561 cctgacgtgg ggcgtcaaca agtcgtctac cgctccagcg ctatcgcaag cggctgatga
    16621 acgcgggggc gagggcgcgg acgcgctgct ccggtacgtc gccgctcaga gcccggacgg
    16681 ccatgtccac accgggagtg gcggcggcgc cgaagagggc gtggagcttt tcgagcccct
    16741 ctgccgccgt gcttccgacc cgggcgtctt tccgaagctc ggcgaaccgg gcggcaacgc
    16801 gaccaggaga ggcagtcatc atgatgcgaa gcacgtcacc ggcgtccttg tcggtgaggc
    16861 ggtcggggcg cttgtccgcg tcatcgagac gatcggtgat cttgaaggcc ttggcgacca
    16921 ggagtgctgt ggggcccgcg acgttcacaa tgatgctgcg ggggtcctcc gcgtcgaggc
    16981 tggagacctg gagaggggag cggtccacgg cggcaacctc aagcccatcg atccaccgtg
    17041 cggacatggc tccgtgcgga gggaccttgg cgctgcgcct gccgttcttc ggggagagct
    17101 gccgagggac gagcaggtcc agttcgactg ggaccactcg gtcgccgatg gtttcggggc
    17161 gctcccagag tccgggctcg tggtcgtact tcagatcgaa accggccgct cggagggagg
    17221 cgtccaggtg cggctggtcg cccagcagca tcgggtcgat gctgaggtct ccgtccgagg
    17281 tgaagggggc gttgctgatc gccgcgtcgg gggagcgaag gtggacggcc tgggcgccga
    17341 cgacggtcag cgcgggaagg tggtcctgca gggcactgag cccatcgatg aggacacgcc
    17401 gggccacgac cgtgagctga cgggggtcga catcgtcagg gaatttggcc acagtgcctc
    17461 aagctctccg tgaaagtgcc aggggtgtaa tcctagagct gatcaggtgt cagcaggttc
    17521 tccatgccgg atacctgcca gcgcctctgg tgggccttca tgtactcgat gacggcctcc
    17581 ccctcggcgg gcaggcggcc cggaccggcc aggcagtcca gggctgcctg gctcaacgcc
    17641 acgtgcggaa tgccgtcgac aatcctggtg cgttcgaaga cggatcggtc gcgtgcttgg
    17701 agcagcagga cgtccgcatt ctcatcgaca ggcaggaggc ccagttcctc accgaggtcg
    17761 tcttcgtcca agatcgggga ggtgtacacc atgagctgtc cgcccgcagc gagaggggcc
    17821 actgtatggg cggcataagt gccggtgacc gcgatgttgt cgagccccga ctgtggcagg
    17881 tgccggatgc gttcgatcac ggctgggacg ccgttcggcg ccagtaggcc cacatacgag
    17941 cctttgcgga gcaaggtctc ccgagcggcg cgtgcccgca gcaggttcgg ccagtcgacc
    18001 gacgtgatga ctcgcccgtc tcggcggatg aggagttggt cctccagcgc gtccagaagc
    18061 cgtgagacat agccgaggct cagccctgag gcctgagcga gttcgcccgc gcggtagggg
    18121 gggtgcacgt cggtgaggag tcgtaccaac ctgcctgcct ttggccctgc cagggtcgtc
    18181 cttcccggct ccttggctag ggcctgcggg gcacgggtgg ccccttcggt gtggatgacg
    18241 atggcgggtc gcgggacccg cagggacacg ttgcccatga ggtccaggta gctgatgtcg
    18301 tgtccgcgca gaagcttctg agtctgccgc gacagccagg gcgtcaggac cagcagggtc
    18361 gtgtggtggt tcagctgtcg cagcagatcc agtttggcca gcaggacctc ttccaccgta
    18421 cgaggtgaca caccggtgag cttcacgtcg accagaagct gagcgtagga accctcaccc
    18481 tcggcttgca cggtcacgac gatgtcgccg ccactacggt gcgtttcctt gttgtccggg
    18541 cgacggtcga tctgccaagg ggggccgagt agctcggcta gtcgggccat gccttgttcg
    18601 aagagggtct ctgaggtcag ctctacggct gtcatgccct tatggtcgca tggcggcatg
    18661 tcgcaaggtg gaataacttg cccttagggc caactttcct gaaccaggca agctggtcca
    18721 tgaggaaagt cttctccttc ctctgacttc tttgccctgg gaaagttggc tctgcaggca
    18781 accgacccac cccagaggtg acctcgtggg tgtggctccg ggctgggatg ggccgtgagt
    18841 gtcctgtggc gaccgtcacc cgtgaaaccc cgcagaccct gtagaccgga tgcccggcgg
    18901 caggcgtata cccggtgaag cgaaagatcc gggagcaact tttgaccgtc gaggagttcg
    18961 aagagttcta cgcccaggcg gtcgcgcgtc tcacaggcca gctgtacgtg atgacggggg
    19021 acctccagga ggcgcaggac gtcgtgcagg aggccttcgt caaggcgtgg gtccggcgcg
    19081 gccggctgga gcgggacggg cagcccgagg cgtggatccg tacggtcgcg tggcgcctgg
    19141 cggtgagccg gtggcgcttc cggcggcgtt cggcggacgc ctgggaccgg gggagcggcg
    19201 cgccggagca cgtggcgccg ccggacccgg accacgtggt gctcgtcgag gcgctccgcg
    19261 gactgcccgc ccagcagcgg cggacgctga ccctgcacta cctgtgcgac ctcacggtcg
    19321 agcagatcgc cggggagacc ggcctttcca ccagcacgat caagacgcat ctggtccgcg
    19381 gccgggcggc gctcgcccac cggctgcggg acccccgcat ggaggaggct cctgatgtct
    19441 gaaccacggg acccgtcggg cccggaggag ccccggggag cgcaggatcc gctgaggacc
    19501 ctgttccacc aggccgggga gttcggccag ggccgcgcgg tcgcggaccc ggcggcccac
    19561 atcaccgaac gcggcatgcg cgcccagcgg cgccggatgg cggcgctcgc ggtgggggtc
    19621 tgcctggtgg tgggcggtgg cggcgcggtg gcggtcggcc tccacccggt caccccgacc
    19681 ccgtcccctc cggcgacgag cccgtccccc agctacccgt ccccctcgcc cccgccgccc
    19741 gccgggactc cgacggagcc gccgacgccg agtacgacga tgccgggggc gcaggggacg
    19801 ggcccggggg cgtactcctc cggttcgtac acgcctggtt cgtccgtgcc cggttcgtcc
    19861 aagccgggag gcatgccgac ggcggaggcg ccgacggctc cgtcgacgtc cacggggccg
    19921 cccggctcga cgccgacctc gacgctcggg tcgacgtcga tgccgtgatc gcggagggcg
    19981 gcgccaggac gacaggcgac ccgcccgcgt aagtactctc cttccttcct cccttccttc
    20041 tccatctacc tcccccgtgc cccggcggct tcaccaggcg atccgggcga tcccgcgtgc
    20101 tctggtcgcg cccgaaaggt gcccaagtgc ctatttcatc ccctgggttg cgcccggccg
    20161 agccccggtc aggctccacc tccgcgcccc ggtcgggcgc cacctccgcg ccccgggcga
    20221 accggccggt caggcccgta ccgtacgcat ccgagtcgcc ccgccgagac cggtgggcat
    20281 cctggctcac cccggccgac cgcagggtgc tccagctcta cctgctgacg cgcctgtccc
    20341 tgtggaccat ggcccacggc gcccggtggc tgtttccccg gggctcggac gtacgggagg
    20401 cggcgccgct gctcgccccg ttccagcagt gggacgcgaa ccactacctc cacatagcgc
    20461 gcgacggcta cttcccggcg ggcgcgggcc cgtggctggg cgactgggac aacagggagg
    20521 cgttcttccc gggctacccg ctcctcctgc gggcggtcca tgtcgtcatc ccggactgga
    20581 cggcggcggg cctgctgatc tcgctggtag ccggggcggt ggccgtgctg gcgctctccc
    20641 gaacagcccg ctcgtacgtc cccgagggcg gagccggtca ccgtacggtc ctgttcttcc
    20701 tgctctcccc gtgcgcggtc ttcctcgcgg ccggctacac ggaggcgctg ttcctggcct
    20761 tcgcgctccc ggcctggctg gcggcccggc gccaccagtg ggcgtgggcc tgcgcgttga
    20821 cggctctggc gacgacggtg cgggtcagcg gcctgttcct ggcggcggcg atagcggtgc
    20881 tgtttgtgct cacggcgctc caggcgggtt cggggaagag gaaggggaag ggacggcgga
    20941 cggcctggcg cggggtggcc tggctggtgc tccccgggct gccgccactg gcgtacagct
    21001 ggtatctgca cgcgcacacg ggcgactgga tgggctggaa gcatgcgcag gagcgcggct
    21061 ggtaccggga gttcctcacc ccgtgggagg cgtgggcgaa cacctggaac gcggccttcg
    21121 gcggtgtgca cacgacgggt tacgcggcga tgttccaggc ggagttggcg gcgatggtgg
    21181 tgggcgtggc cctcgtcgcg ctgctcgcac ggtatggccg atggccggag gcggtgtatg
    21241 tggcgctgag cctgtgggcg ctgggaacgt cgtactggta cacatcgatc ccccgggcga
    21301 cgctgctgtg gtggccgctg tggatcggcc tggcggccgt gagcctgcgc cgcccctggt
    21361 tccgtacggc gtacctgtgc gtctcggccc cgctggcggc gctggtggcc ctggcgttct
    21421 tcacggggag gtgggcgggg tgacaagggt gaccgatcaa ttctccttac agctccgcga
    21481 tatggctctc tgcaggccgt ggacgacccc tccggccacg aggacggcgg cgcccgtgac
    21541 gacgctgccc ggggggaggg cgaaggccag ggcggtacaa ccggccaggc cggtgagggg
    21601 gatgagacgg ttcggccggc cctcctcggc ggacagggtc caggcggagg cgttggcgat
    21661 ggcgtagtag gccaggacac cgaaggacga gaagccgatc acctcgcgta cgtcggcggt
    21721 cgcggcgagc acgacgacaa ccgtgcccac ggccagctcg gcgcggtggg ggacgccgaa
    21781 gcgggggtgg acggcggcca gggcgtgcgg caggtgccgg tcccgggcca tggccagggt
    21841 ggtacgggag actccgagga tcagcccgag cagcgccccg agcgctgcga cggcaccgcc
    21901 gacgcggacg accggcacca gccagtcggc gcccgcggct cgcgcggcgt ccgacagcgg
    21961 tgcggtggcg gcggccagac cgtgcgggcc cagcgccgcc aggacggcga cggcgaccag
    22021 gccgtagacg gcgagggtga tggcgagtgc gacggggatg gcacgcggaa tcgtccgcgc
    22081 cggatcgcgg acctcctccc cgagagtggc gatacgggca taaccggcga aggcgaagaa
    22141 cagcagcccc gctgcctgga gcaccccgcc catggtgacg tccgggccga tgccgagtcg
    22201 cgcggcgtcc gcctcggcgg agccgagcgc ggtgaccacg acggccgcga gcacggccag
    22261 aaccgtacag accacgatgc gggtgaggaa gacggacttc tgcaccccgg cgtagttcac
    22321 cgcggtcagc gccacgaccg cggccacggc cacggcgtgg gcctggcccg gccagaggta
    22381 agcaccgacg gtgagggcca tggccgcgca ggaggcggtc ttgccgacca cgaacgccca
    22441 cccggccagg tagccccaga actcccccag gcgctcgcgg ccgtagacat aggtgccgcc
    22501 ggactcggga tacagggcgg ccagtctggc cgaggaggtc gcattgcagt aggccacgac
    22561 cgaggccagc cccagcgcga gcaacagccc ggagccggcg gcgtcggccg cgggtccgag
    22621 cgccgcgaag atcccggccc ccaccatgga cccgagaccg atgacgacgg cgtcggagag
    22681 ccccagacgc cgctccatcc cccgtgcgcc ggctggggcg ccactcacgt cgaccatcgg
    22741 cttcctctcg tgcgaccgcg cgaactgtgg cgcacgctag cgcaggaaga tgtcggc
//
