LOCUS       NZ_KB890918            10399 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. CcalMP-8W B053DRAFT_scaffold_11.12, whole genome
            shotgun sequence.
ACCESSION   NZ_KB890918
VERSION     NZ_KB890918.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. CcalMP-8W
  ORGANISM  Streptomyces sp. CcalMP-8W
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 204168)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
REFERENCE   2  (bases 1 to 204168)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. CcalMP-8W
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 03:14:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,603
            CDSs (total)                      :: 6,529
            Genes (coding)                    :: 6,424
            CDSs (with protein)               :: 6,424
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 1 (5S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 83 of 105
            Pseudo Genes (internal stop)      :: 5 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:04:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 125997
            Orig. end                         :: 136396
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB890918.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405361
            Source DNA and Organism available from Cameron  Currie
            (currie@bact.wisc.edu)
            Source DNA available from Cameron  Currie (currie@bact.wisc.edu)
            Organism available from Cameron  Currie (currie@bact.wisc.edu)
            Contacts: Cameron  Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(434..994)
                     /locus_tag="B053_RS0108345"
     CDS             complement(434..994)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981966.1"
                     /locus_tag="B053_RS0108345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_050565982.1"
                     /transl_table=11
                     /translation="MRTGRRALDRLDEEQLVRRVSRGDLAAFEELYRRTSPWLATRLRR
                     RCADEQIVAEVMQETYLAVWRAAAGFVGTATGGTAVGWLWTIAARRLVDAFRRRAHHAA
                     PPSAAAPAPTAPAAEEEALSASVGGDIGDALSALAPELREVLRALVLDGLTVRETAVLL
                     GLPEGTVKTRARRARIAMRRALA"
     misc_feature    complement(1037..1052)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            complement(1109..1615)
                     /locus_tag="B053_RS0108350"
     CDS             complement(1109..1615)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607784.1"
                     /locus_tag="B053_RS0108350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_018488166.1"
                     /transl_table=11
                     /translation="MNTAHAGAAEAACTDALPGAALSGPVSHAVSRVARLHRIAAGKLL
                     KGLGLYPGQELLMMHLWDAGPVRQSELIRTLDLDPSTVTKMLQRLEQTGHVRRRPDPDD
                     RRAALVEATEASCALHRAVRDAWGSLEEHTLAGLDDGERTELTRLLAKVEANLCRETEG
                     CPEGS"
     misc_feature    1831..1845
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.72"
                     /tool="antismash"
     gene            1870..2940
                     /locus_tag="B053_RS0108355"
     CDS             1870..2940
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="B053_RS0108355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alkene reductase"
                     /protein_id="WP_018488167.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLAGTRLANRIVMAPMTRSRAGEGGTATELTVAYYTQ
                     RASAGLIVTEGIQPSAVGQGYPFTPGLHSAEQVASWRTVTDAVHAEGGRIFAQIMHAGR
                     IGHPVLLPDGLVPVAPSPVRAAGQVYTAEGPKDFVEPHELTDAEIRQTIADFAAAARNA
                     VEAGFDGVELHGANGYLIHQFLAPNTNLRTDGWGGSESARVRFAVEVVKAVAAEIGGAR
                     TALRISPSNPYNDIDEPAPDAVYTALAQEIEPLGLAYLHILEDAPIRELTLQLRKVFTG
                     SVLINVHSDGPTGPDDHTVIDDGIADLISYGVLFLANPDLPARLKAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDAV"
     gene            complement(2916..3950)
                     /locus_tag="B053_RS0108360"
     CDS             complement(2916..3950)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="B053_RS0108360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_018488168.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADPYTLGTFWSRVLGAPLAPDDFPGDPEALLET
                     PGAAILLVRNDDPKAVKNRVHLDVQPQDRTRDEEVERLLALGATLVGDHRRADGRGWAT
                     LTDPEGNEFCVECSAAERAALTGARLPVTADDVTTAVRLAVDALAGAPAEGWDAPAGTL
                     EWTCWETVEHLSDDLFAYAVQLGPRTPPLDGEVPYRWAPERPGGPYNAVFADRAAGPAG
                     LLATLEASGALLASMARTTPPEVRSYHGFGVSDPEGFAAMGVVETLVHTHDVAAGLGLD
                     WAPPAVLCDRVLARLFPGAPAGGDRWTVLLWATGRAELPGHPRRTAWRWDGRPPADQTA
                     SSAG"
     gene            complement(4102..4995)
                     /gene="thpD"
                     /locus_tag="B053_RS0108365"
     CDS             complement(4102..4995)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="B053_RS0108365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_018488169.1"
                     /sec_met_domain="PhyH (E-value: 4.4e-39, bitscore: 128.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAARDLQSY
                     EHDGFLTVDQLITPDEVSVYRAELDRLVADPLIRADERSIVEKQSQNVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RAVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENTAQEPFAAP
                     IRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="B053_RS0108370"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="B053_RS0108370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_018488170.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.1e-55, bitscore:
                     179.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWISPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            complement(5489..6751)
                     /gene="ectB"
                     /locus_tag="B053_RS0108375"
     CDS             complement(5489..6751)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 3.9e-72.
                     Score: 235.0. Matches aSDomain:
                     nrpspksdomains_B053_RS0108375_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="B053_RS0108375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_018488171.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-78, bitscore:
                     258.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCERQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGRVEQQTKERGLQVEQALL
                     AICAEEPTAQFRGRGLVWGMEFEDPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITTEELDEGLRTLARCVRETA"
     aSDomain        complement(5663..6667)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B053_RS0108375_Aminotran_3.1"
                     /evalue="3.90E-72"
                     /label="B053_RS0108375_Aminotran_3.1"
                     /locus_tag="B053_RS0108375"
                     /protein_end="363"
                     /protein_start="28"
                     /score="235.0"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCERQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGRVEQQTKERGLQVEQALLAICAEEPTAQFRGRGLVWGMEFE"
     gene            complement(7077..7607)
                     /gene="ectA"
                     /locus_tag="B053_RS0108380"
     CDS             complement(7077..7607)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121394.1"
                     /locus_tag="B053_RS0108380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_026237552.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDTPRVEDGAAIWRIARDSEVLDLNSSY
                     SYLLWCRDFAATSVVARGEDGEPIAFVTGYIRPDRPETLVVWQVAVDGAHRGKGLAATL
                     LDALTARVAADQGLSWVETTITPDNTASDRLFTSYAQRHDVALEREVLFDGGLFPEGTH
                     LPEVLYRIGPLTG"
     misc_feature    7618..7632
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7632..7644)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7719..7734
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.16"
                     /tool="antismash"
     misc_feature    7729..7743
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.76"
                     /tool="antismash"
     misc_feature    7773..7788
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7773..7790)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7848..7864
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 21.16"
                     /tool="antismash"
     gene            complement(7924..9030)
                     /locus_tag="B053_RS0108385"
     CDS             complement(7924..9030)
                     /NRPS_PKS="Domain: Aminotran_5 (74-284). E-value: 4.8e-29.
                     Score: 93.3. Matches aSDomain:
                     nrpspksdomains_B053_RS0108385_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="B053_RS0108385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_018488173.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.1e-23, bitscore:
                     77.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADRFAAIERRVAALLATGQDVVITQGEALLPLE
                     GCIRSGARPGSAALNVVTGPYGQTFGNWLRDCGAQVVDLTVPFHTAVTADQVAEALAGH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWERFAANPAAPRRSYLSLLDWKERWIDAGRKALPHAP
                     AQLEMLGLEACLERIEEEGPDHVMARHAAAAAATRAGAVALGGGLEPYVHEARDAAPVA
                     TTLRAPAGTDAAALVARALTTEPSLPLVAGGGALSKEMIRVNHYGADATRSAVLSSLAA
                     LGAALGDTGRQVDFDAARRAVSETSPDL"
     aSDomain        complement(8179..8808)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B053_RS0108385_Aminotran_5.1"
                     /evalue="4.80E-29"
                     /label="B053_RS0108385_Aminotran_5.1"
                     /locus_tag="B053_RS0108385"
                     /protein_end="284"
                     /protein_start="74"
                     /score="93.3"
                     /tool="antismash"
                     /translation="RDCGAQVVDLTVPFHTAVTADQVAEALAGHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWERFAANPAAPRRSYLSLLDWKERWIDAGRKALPHAPAQLEMLGLEACLERI
                     EEEGPDHVMARHAAAAAATRAGAVALGGGLEPYVHEARDAAPVATTL"
     gene            9180..10292
                     /locus_tag="B053_RS0108390"
     CDS             9180..10292
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607776.1"
                     /locus_tag="B053_RS0108390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_050565983.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.3e-20, bitscore:
                     66.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQVRDQERQPVLRVKGRVLVGPDEVRDELWAVDGRITFDRPPD
                     ADRAETVTGWVLPGLVDAHCHVGLDRHGPVDGATAEKQALTDREAGTLLIRDAGSPSDT
                     RWIDDREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDW
                     IDRSAGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEESLRDLVEAGIDCIEHATGLT
                     EETVPLFAERGVAIVPTLVNIATFPDLAAGGEDKFPRWSAHMRQLYERRYDTVRAAYDA
                     GIPVYVGTDAGGSLAHGLVAGEVAELVKAGIPPLEALSATTWGARRWLGRPGLEEGAPA
                     DLVVYEEDPRADVRVLAAPRHVVLNGRVVG"
ORIGIN
        1 tgggcggggt ccgtcacccg cgaccaggac acggcgacgc cgagcaccgg cagcaggggt
       61 gcgatcagga cgaccgggga caccgccccg gtgcggtgtc cggccaggtc catcaggagc
      121 gcgagccccg tcaggcccac caccgtggcc agccacggca gcgcggccgg tgtcatccag
      181 cgcgccgggc gcatccggcc gtgccgtccc gccccggctc ccggggccgc cagctcggcg
      241 gcgagcccgg tccacacgct ccgggtgagc gccgtgacgg cggggacctg accggccacc
      301 gcctccgaca gcagcctgcg gcagtcggcg cacgcttcca gatgggattc caccgcccac
      361 aggacgtcgg cgggcagcgc cgtgtcgccc cgcgcgtagg cggtgaggat gtggggtgat
      421 gcgtgtcctg cggtcatgcc agcgccctcc gcatggcgat ccgggcccgc cgtgcgcggg
      481 tcttgactgt gccctcgggc agccccagca ggaccgcggt ctcgcggacc gtcagcccgt
      541 cgagcaccag ggcccgcagc acctcgcgca gttccggcgc gagcgcgctc agcgcgtcac
      601 cgatgtcacc gccgacgctc gcgctcagcg cctcctcctc ggcggccggc gcggtcggtg
      661 ccggagcggc cgcggagggc ggcgcggcgt ggtgggcgcg gcggcggaag gcgtcgacca
      721 gacgccgtgc cgcgatcgtc cacagccagc cgacggccgt tccgcccgtc gcggtgccga
      781 cgaagcccgc cgccgcgcgc cagacggcga ggtaggtctc ctgcatgacc tcggcgacga
      841 tctgctcgtc ggcgcagcga cggcgcaggc gggtggcgag ccagggggag gtgcgccggt
      901 acaactcctc gaacgcggcg agatcgcccc tgctcactct ccggacgagc tgttcctcgt
      961 ccagccggtc gagtgctctt ctgcctgttc tcacacctgc tagacgtggg gatccgcccc
     1021 ggggttttcc tcctcgtgtg acgtgggtca cctcggcggc gtcctggcgg cgcccggggt
     1081 gcttcccgcc cccgcccccc ggggagggtc agctcccttc cgggcagccc tccgtctccc
     1141 ggcagaggtt cgcctccacc ttcgccagca gccgcgtcag ctccgtgcgc tcgccgtcgt
     1201 ccagcccggc gagcgtgtgc tcctccaggc tgccccacgc gtcccgtacc gcccggtgca
     1261 gcgcgcagct cgcctccgtc gcctcgacca gggcggcccg ccggtcgtcc gggtcggggc
     1321 ggcggcgcac atgaccggtc tgctccaggc gctggagcat cttggtcacc gtggaggggt
     1381 ccaggtccag cgtcctgatc agctcggact ggcgtaccgg ccccgcgtcc cacaggtgca
     1441 tcatcagcag ctcctgcccc gggtagagcc ccagcccctt cagcagcttc cccgccgcga
     1501 tccggtgcag ccgggcgacg cgggagacgg cgtggctgac gggcccgctg agcgctgctc
     1561 ccggcagcgc gtcggtacag gcggcttccg cggcgccggc gtgggcggtg ttcatggcgg
     1621 ggctccttcg gtcctgctcc ggcggggctt ccgccggttc ctggcgggtt cggtcccgga
     1681 cgggcgacgg gttcggtcct ggcgggtccg ggggccggcg gcggctcccg gggtccggtg
     1741 gcgcccgagg cttgcccaca ctttaccttg gtcggccaat taatgagcta cagtgacgtc
     1801 agcgccaata cttggccgac cacatacttt gtttatcggt caattaccca ccttttcccg
     1861 ggagttcgca tgaccaccgc tttcgacccg atcgacctgg ccggcacccg cctggccaac
     1921 cgcatcgtga tggccccgat gacccgcagc cgggcgggcg agggcggtac ggccaccgag
     1981 ctgaccgtcg cctactacac ccagcgcgcc tccgccgggc tgatcgtcac cgagggcatc
     2041 cagccctccg cggtcggcca gggctacccc ttcacccccg ggctgcacag cgccgagcag
     2101 gtcgcctcct ggcgcacggt caccgacgcc gtgcacgccg agggcggccg gatcttcgcc
     2161 cagatcatgc acgcgggccg catcggccac ccggtcctgc tccccgacgg actcgtcccg
     2221 gtcgccccct ccccggtccg ggccgcgggc caggtctaca ccgccgaggg ccccaaggac
     2281 ttcgtcgagc cgcacgagct gaccgacgcc gagatccggc agaccatcgc cgacttcgcc
     2341 gccgccgccc gcaacgccgt cgaggccggg ttcgacggcg tggagctgca cggcgccaac
     2401 ggctacctga tccaccagtt cctcgcgccc aacaccaacc tgcgcaccga cgggtggggc
     2461 ggctcggaga gcgcccgcgt ccggttcgcg gtggaggtcg tcaaggcggt cgcggccgag
     2521 atcggcggag cccgtaccgc cctgcggatc tcgccgtcca acccgtacaa cgacatcgac
     2581 gagcccgccc cggacgccgt ctacaccgct ctggcccagg agatcgagcc gctcggcctc
     2641 gcctatctgc acatcctgga ggacgcgccg atccgcgagc tgaccctcca gctgcgcaag
     2701 gtgttcaccg gctccgtcct catcaacgtg cactccgacg ggccgaccgg gccggacgac
     2761 cacaccgtga tcgacgacgg catcgccgac ctcatctcgt acggcgttct cttcctcgcc
     2821 aacccggacc tccccgcccg cctcaaggcc gggggcccct tcaacacccc cgacccgtcg
     2881 accttcttcg gcggcgacgc caagggctac accgactacc cggcgctcga cgccgtctga
     2941 tccgccgggg gccgcccgtc ccaccgccac gcggtgcggc gcgggtggcc cggcagttcc
     3001 gcgcggccgg tggcccagag cagcacggtc caccggtcgc cgcccgccgg ggcgccgggg
     3061 aagagccggg ccagcacccg gtcgcagagc acggccggcg gcgcccagtc cagccccagc
     3121 cccgccgcga cgtcatgggt gtgcaccagg gtctccacca cccccatcgc ggcgaacccc
     3181 tccgggtcgg agacgccgaa cccgtggtac gagcgcacct ccggcggcgt cgtccgcgcc
     3241 atcgaggcca gcagcgcacc gctcgcctcc agggtggcca gcagcccggc gggcccggcc
     3301 gcccggtccg cgaagaccgc gttgtacggc ccgcccggcc gctcgggcgc ccaccggtag
     3361 ggcacctcac cgtcgagcgg cggcgtacgc gggcccaact ggacggcgta cgcgaagaga
     3421 tcgtcgctca ggtgctccac cgtctcccag caggtccact ccagcgtccc cgcgggcgcg
     3481 tcccacccct ccgccggggc cccggccagc gcgtccaccg cgagccgcac ggcggtggtc
     3541 acgtcgtcgg cggtcaccgg cagccgggcc ccggtcagcg cggcccgctc ggcggcggag
     3601 cactccacgc agaactcgtt gccctccgga tccgtgaggg tcgcccaccc ccgcccgtcc
     3661 gctcggcggt ggtcgcccac gagcgtggcg cccagcgcga gcagccgctc cacctcctcg
     3721 tcccgggtgc ggtcctgcgg ctggacgtcc aggtggaccc ggttcttgac ggccttaggg
     3781 tcgtcgttcc gcacgaggag gatcgccgcg ccgggggtct ccagcagcgc ttccgggtcg
     3841 ccggggaagt cgtccggggc cagcggcgcg ccgagcaccc gggaccagaa ggtgccgagc
     3901 gtgtaggggt cggcgcagtc gatcgtgatg tgccgtacgt gagaagtcat ggccgccacc
     3961 ctctccgccg ccccggggcc cggtccaccg ggttacgcgc gagggccggg aacgggtcac
     4021 gcgcgagggg ccggcgcggg gcacacggcg ggggcccggg acggtgtgcc gtcccgggcc
     4081 cctcgcgcgt ggcacgtccc gctacttcac cggggtgaag tcccgcgccc cgatgaactc
     4141 cgggcggcgg atgggtgccg cgaacggctc ctgcgccgtg ttctccacgc tgttgaagac
     4201 gatgaagacg ttggagcggg cgtacggggt gatgttgtcg cccgagccgt gcatggcgtt
     4261 gcagtcgaac caggtcgccg aaccggcctt gcccgtgaag agcctgatgc cgtggcggtc
     4321 cgccatcttg gtcagcgcct cgtcggacgg ggtgcccgcg tcctgcatct ggagcgactt
     4381 cttgtagttg tccttcggcg tctcgcccgc acagcccagg aaggtcttgt gcgaaccggg
     4441 catgatcatc agcccgccgt tggtgtcgaa gttctccgtc agcgcgatcg acaccgacac
     4501 ggcccgcatg ttcggcagcc cgtcctcggc gtgccaggtc tcgaagtccg agtgccagta
     4561 gaagcccgag gcgccgaacc ccggcttcac gttgatccgg gactggtgga cgtacacgtc
     4621 cgagccgagg atctggcggg cccggcccac cacccgctcg tcgcggacca gcccggcgaa
     4681 gacctcgctg atcctgtgga cctcgaagac ggaccggacg ttctgcgact gcttctccac
     4741 gatggagcgc tcgtcggcac ggatcagcgg gtcggcgacc agccggtcca gctccgcgcg
     4801 gtagacggag acctcgtccg gggtgatgag ctggtcgacg gtgaggaagc cgtcgtgctc
     4861 gtagctctgg agatccctgg cggcgaccgg acccggtgcg cccggcgccg accagatgac
     4921 cgggtcctgg cggggagtgg tcatctcggc ggcgccgcgc gaggggtaca gatcggcgcg
     4981 tacttcggtg gtcatacggt tcaggcctcc tcggtcagca gtgggtagac accgttctcg
     5041 tcgtggtcct cccgtccggt gacgggaggg ttgaagacgc acacgcagcg gaagtcggtc
     5101 ttgggccgca gggtgtgccg ctcatggccg tccagcaggt acatcgtgcc gggggagatc
     5161 cagtgggtct cgccggtctc gtcgttggtc agctcggcct cgccctcggt gcacaggacg
     5221 gcctcgatgt ggttcgcgta ccacatcgag gtctccgtgc ccgcgtagag cacggtctcg
     5281 tggagggaga agccgacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgcggcct tcacatgccg gtcggtgttc tcgatgtcct tgaaggatcg gacgatcacg
     5401 gtgagcggtg cctttctctc gtgctccgcc ccgggccgcg aggcccgggg cgcctcttct
     5461 tcctgtcgcc tgccccggcc gcgccgggtc aggccgtctc gcggacgcag cgggccagcg
     5521 tgcgcaggcc ctcgtccagt tcctcggtgg tgatggtcag cggcggcagc agcttgacga
     5581 cctcgctctg cgggcccgag gtctccagca ggagccccag ctcgaaggcg cgggcgcaga
     5641 ccgccgaggc gcgcgccggg tcctcgaact ccatgcccca gaccaggccc cggccgcgga
     5701 actgcgcggt cggctcctcg gcgcagatcg ccagcagcgc ctgctcgacc tggaggccgc
     5761 gctccttggt ctgctgctcc acccggccgt cggcccagta ggcgtccagc gcggcggcgg
     5821 ccgtgacgaa ggccgggttg ttgccgcgga aggtgccgtt gtgctcgccc ggctcccaga
     5881 tgtccagctc cggcttgaac aggcagagcg acatcggcag gccgtagccg ctgatggact
     5941 tcgacagcgt gacgatgtcc gggacgatcc cggcctcctc gaaggagaag aagccgcccg
     6001 tccggccgca gcccatctgg atgtcgtcga cgatcagcag catgtcctgg cgctcgcaca
     6061 gctcctggag cgcgcgcagc cactcggcgc gggccacgtt gatgccgccc tcgccctgca
     6121 cggtctccac gatcacggcg gcgggcttgt tgagcccgga gccctggtcc tccagcagcc
     6181 gctcgaacca gaggaagtcg gggaccgtgc cgtcgaagta gttgtcgaac ggcatcggcg
     6241 tgccgtgcac cagcgggatg ccggcgcccg cccgcttgaa cgcgttgccg gtgacggcga
     6301 gcgagcccag cgacatgccg tggaaggcgt tggtgaacga cacgacggac tcgcggccct
     6361 tgaccttgcg ggccagcttc agcgccgact cgacggcgtt ggtgcccgtc gggccgggga
     6421 acatcacctt gtacggcagg tcgcgcgggc gcaggatcac gttctggaac gtctccagga
     6481 aggcgcgctt ggccgtggtc gccatgtcca ggccgtgggt gatgccgtcg cgctcgatgt
     6541 agtcgatcag cgctcgtttc agcacggggt tgttgtggcc gtagttgagc gagccggccc
     6601 cggcgaagaa gtccaggtag gagtggccgt cctcgtcggt cagccgggcg ccctgcgcgc
     6661 ggtcgaacac ggcgggccag ccgcggcagt agctacggac ctccgactcc agggtctcga
     6721 agacgctcag tgcgggcggg gtgatggtca cagcggatct cctgcgtgag ggggtgtgac
     6781 gggcggtgtg gtcggtgctc ccggcccctc gggggcggcc gggaccgcgc cgggtggtcg
     6841 gccggcgcga aaagtcgtgg gggaccgggc gtacgggtgc ggtacgccgg tcgtacgggg
     6901 gtgtacgggc cgtgcggtcg tacgggaccg tccgggcccg ggcattccgg gcgggccggg
     6961 cgggtcgtct ccggcggccg ggcggtggcc cggccggtgg ggccgggtgc ttcggacgcc
     7021 cggggccgcc gcagcggcgc cccggtgcct cagggctcgg cggcgcgccg ggcacctcag
     7081 ccggtgagcg gtcctatccg gtagaggacc tcgggcaggt gcgtcccctc ggggaacagc
     7141 ccgccgtcga agagcacctc ccgctccagc gccacgtcgt gccgctgggc gtaggacgtg
     7201 aacagccggt cggacgcggt gttgtccggg gtgatcgtcg tctcgaccca ggagagcccc
     7261 tggtcggcgg cgacccgggc ggtcagcgcg tccaacagcg tggcggccag ccccttgccg
     7321 cggtgcgctc cgtcgacggc cacctgccag accaccaggg tctcggggcg gtcgggccgg
     7381 atgtagccgg tgacgaaggc gatcggctcg ccgtcctcgc cacgggcgac gacggacgtc
     7441 gcggcgaagt cacggcacca cagcaggtag ctgtacgagg agttcaggtc caggacctcg
     7501 gagtcgcggg cgatgcgcca gatcgcggct ccgtcctcca ctcgtggcgt gtcgatactc
     7561 aggaattcgc ttcgggcacg ggcaaaatct gctggtgcgg cggtcatgtg aattgaattt
     7621 acccagcaaa tttcgaaatt gcatcgaggc aaggggttgg gtggagcgct cgtctatgtt
     7681 atcaggcgtg cgtgagaccc ggggtgatca gggggcgatt cagtgctttt tgtcctgaat
     7741 ttttcgggca taacgccgag ctagtggagt cggtcacaag gtggtaactc cttcggaacg
     7801 tgtccgaatc atgaagttcg ggccccggga aaacttccgc gtttagggga aggaaaagcg
     7861 ggcagaagaa tgcggggagc tgatttcgat aaagcagctc cccgcattgt gaatatcaga
     7921 ggctcagagg tccggagagg tttccgagac cgcgcggcgg gcagcatcga agtcgacctg
     7981 ccggcctgtg tcgcccagcg ccgcccccag ggccgcgagc gaggacagca ccgcgctccg
     8041 cgtcgcgtcc gccccgtagt gattgacccg gatcatctcc ttggacagcg ccccgccgcc
     8101 cgcgacgagc ggcagcgagg gttccgtcgt gagcgcccgc gcgaccagcg cggcggcgtc
     8161 ggtccccgcc ggggcgcgca gcgtggtggc caccggtgcg gcgtcccgcg cctcgtggac
     8221 gtacggctcc agcccgccgc cgagggccac cgcgcccgcc cgggtcgccg ccgcggccgc
     8281 ggcgtgacgg gccatcacgt ggtccggtcc ctcctcctcg atccgctcca ggcacgcctc
     8341 caggcccagc atctccaact gcgccggggc gtgcgggagc gccttgcggc cggcgtcgat
     8401 ccagcgctcc ttccagtcca ggagcgagag gtaggagcgg cgcggggcgg ccgggttggc
     8461 ggcgaaccgc tcccaggccc gctcgctcac cgacaccgcc gagaccccgg ccggaccgcc
     8521 catcgccttc tgcgcgccga tcacgcacag gtccacgccc caggcgtccg gcagcagcgg
     8581 ctcggcgccc accgaggcga ccgcgtccag catgaagagc gccccgtgcg cccggaccac
     8641 ctcaccgatc tccgcgaccg ggttggtgtt gccggtggcc gcctccgcgt gcaccagcga
     8701 gacgaagtcg atctccgggt gcccggcgag cgcctcggcg acctggtcgg cggtgaccgc
     8761 cgtgtggaag ggcacggtga ggtcgacgac ctgggcgccg cagtcccgca gccagttgcc
     8821 gaaggtctgc ccgtacggcc ccgtgaccac gttcagcgcg gccgagccgg gccgggcgcc
     8881 gctgcggatg cagccctcca gcggcagcag cgcctcgccc tgcgtgatga cgacgtcctg
     8941 cccggtggcg aggagcgcgg ccacccgccg ctcgatggcc gcgaagcggt cggcggtcag
     9001 cgggggcagg tcgaggaagg ggtgcgtcac ggtggggctc tcttcgggtc ggggccgtgg
     9061 tcgggcggcg gtgctgcgga gcggtcggcg ccccctcggg ggagtgccgc gcccaggggt
     9121 gtccggctcg gtcccgacgc ccccctcgca gcctaccgat ggcctcgtac agtgccgcta
     9181 tgagtgatca ggtgcgcgat caggagcggc agccggtgct gcgcgtgaag gggcgggtcc
     9241 tcgtcggccc ggacgaggtg cgggacgaac tgtgggccgt cgacgggcgg atcaccttcg
     9301 accggccgcc ggacgcggac cgcgcggaga ccgtcacggg ctgggtgctg cccggtctcg
     9361 tcgacgcgca ctgccatgtc ggcctggacc ggcacggccc ggtcgacggg gcgaccgccg
     9421 agaagcaggc actcaccgac cgggaggccg ggaccctgct catccgggac gcagggtcgc
     9481 cctccgacac ccgctggatc gacgaccgcg aggacctgcc gaagatcatc cgggcgggcc
     9541 gccacatcgc caggacccgc cgctacatcc gtaactacgc ccacgagatc gagcccggcg
     9601 acctcgtcgc gtacgtggcg cgggaggccc ggcgcgggga cggctgggtc aagctggtgg
     9661 gggactggat cgaccggtcc gccggggacc tcaccgcctg ctggccgcgc ggcgaggtgg
     9721 aggcggccat cgcggaggcg caccggctgg gcgcccgcgt caccgcgcac tgtttcgcgg
     9781 aggagtcgct gcgcgatctg gtcgaggcgg gcatcgactg catcgagcac gccaccggcc
     9841 tcaccgagga gaccgtcccg ctcttcgccg aacgcggggt cgccatcgtc ccgaccctgg
     9901 tcaacatcgc cacgttcccc gacctcgcgg cgggcggcga ggacaagttc ccccgctggt
     9961 cggcccatat gcggcagctg tacgagcgcc ggtacgacac cgtgcgcgcc gcgtacgacg
    10021 ccggcatccc ggtctacgtc ggcaccgacg cgggcggctc gctggcgcac ggcctggtcg
    10081 cgggcgaggt ggccgagctg gtgaaggcgg gcatcccgcc cctggaggcc ctctccgcca
    10141 ccacgtgggg ggcgaggcgg tggctggggc ggccgggcct ggaggagggc gctccggcgg
    10201 acctggtcgt gtacgaggag gacccgcgcg ccgacgtccg ggtgctggcg gccccgcgcc
    10261 atgtggtgct caacggtcgg gtggtggggt gagccggggc cggggcgccg ggtggcgacg
    10321 ggttgaccgg gggtgacgta accggccccc ggctcgttga acgcctttac gcggcccccg
    10381 gtacggtcgc acgccgttc
//
