LOCUS       NZ_CM001792            10387 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Oceanobacillus kimchii X50 chromosome, whole genome shotgun
            sequence.
ACCESSION   NZ_CM001792
VERSION     NZ_CM001792.1
KEYWORDS    WGS; RefSeq.
SOURCE      Oceanobacillus kimchii X50
  ORGANISM  Oceanobacillus kimchii X50
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Oceanobacillus.
REFERENCE   1  (bases 1 to 3830781)
  AUTHORS   Kim,J.Y. and Bae,J.-W.
  TITLE     Genome Sequence of Oceanobacillus kimchii X50T, Isolated from
            Traditional Korean Fermented Food
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3830781)
  AUTHORS   Kim,J.Y. and Bae,J.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2012) Department of Life and Nanopharmaceutical
            Sciences and Department of Biology, Kyung Hee University, HoeGi-Dong
            1, DongDaeMun-Gu, Seoul 130-701, Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. 2.6; CLC NGS Cell v. 5.0
            Genome Coverage                   :: 267.13x
            Sequencing Technology             :: 454; Illumina GAIIx; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000340475.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 09:35:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,833
            CDSs (total)                      :: 3,769
            Genes (coding)                    :: 3,736
            CDSs (with protein)               :: 3,736
            Genes (RNA)                       :: 64
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 18 of 33
            Pseudo Genes (internal stop)      :: 8 of 33
            Pseudo Genes (multiple problems)  :: 7 of 33
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:23:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 469393
            Orig. end                         :: 479780
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CM001792.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jin-Woo Bae, Kyung Hee
            University, HoeGi-Dong 1, DongDaeMun-Gu, Seoul 130-701, Korea.
FEATURES             Location/Qualifiers
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5387](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5387
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            15..704
                     /locus_tag="C794_RS02635"
     CDS             15..704
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064918.1"
                     /locus_tag="C794_RS02635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_017795594.1"
                     /transl_table=11
                     /translation="MIELTDVYKRFGRKSVLNGVTFQAEKSAITCLIGINGSGKTTILQ
                     SIMNLTPISKGKITIDGNLLNKNTYEKLTYIPDTITLLPRMKIKEAFIFMDDFYSNWNQ
                     KRADEMLSFFKLDREERIMNLSKGNKSKVNMLMGLAMDADYILMDEPFSGIDIFSREQI
                     TDVFTSHLVEDRGVIITTHEINDIEHLIDKAILIDNGVVVKEFNTEQLREDEGKSVVDV
                     MREVYRT"
     gene            701..1525
                     /locus_tag="C794_RS02640"
     CDS             701..1525
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064919.1"
                     /locus_tag="C794_RS02640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_017795595.1"
                     /transl_table=11
                     /translation="MSNFGKLVNFEFRRFLPIFLPLVGILVLSQVIGAIVFSMRYLSDM
                     ERYLIQGQRTTEDFILEFGKLSMMEVVNSIYFMGPIGVAVVSLLIYLLFIWYRDWIGKN
                     TFAYRLLTLPTNRLNLFFSKASTIFIFIFGFITIQLIMMPILQFILKLFVPVDYRLDMA
                     LGAMVQANYFLSILLPQTISGFFIHYGLGIVFTIGIFTMVLLERSYRLIGIGFAFLYAI
                     IVCTFIFTPYYFQYFFHLYTHEAFWLETSVMIIISVLSIWLSSHLLNNKIRV"
     gene            1532..2743
                     /locus_tag="C794_RS02645"
     CDS             1532..2743
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C794_RS02645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_017795596.1"
                     /transl_table=11
                     /translation="MKHIKKYWGLWAIIITSTIAIVIYYVHMETSKINLEWKTVSGEEK
                     IVEDLSIKGHLFTPYQEDYFSSTPNNFERSSQENVLLSNGLSIYQHERLNRFIDEYQSF
                     MRGKSMDDSLYHETDEKIIYTSVDDEEENLLIELLDKQTKEVYKFEYPFENIEGSYISV
                     NSTEYVEDELYIIISSLDINGEAGENYEYYLYTIDMKNNQLVEERKIIGKEDQEKTDII
                     QLIDDTFTENQKVVMFEKYNSPELISYTNENGEYVSERSMDDSDVEDVRQLFLLDLINN
                     EKIEIPNTIALGSNHIIRNQFVYYLREEAEARYIEQYNINKQEIDTIATLNGEDTGYGT
                     LLQEKNGYMYIANLNSNLEINLEVINIENGDSSYKGHIVVSELSEQLLKEQIQIMPQEL
                     EFRY"
     misc_feature    complement(3081..3096)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.53"
                     /tool="antismash"
     gene            3157..3705
                     /gene="ectA"
                     /locus_tag="C794_RS02650"
     CDS             3157..3705
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /locus_tag="C794_RS02650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_026133697.1"
                     /transl_table=11
                     /translation="MLDVAEKLKVDTDEYYFRRPTKEDGGPVWELVREIGNLDLNSSYS
                     YVLWCEVFAESSIVVEHKETQQIVGFISGFMHPEKEDTLFIWQVAVSSTQRGKGLATKM
                     LLQLLEWNESVNFIEATVAPSNKPSNYLFLGLARKIHTNWKISDYFKTEHFPAKDEEHE
                     EELLFRIGPMRKNKNKRMI"
     gene            3705..4985
                     /gene="ectB"
                     /locus_tag="C794_RS02655"
     CDS             3705..4985
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (32-365). E-value: 1.2e-79.
                     Score: 259.7. Matches aSDomain:
                     nrpspksdomains_C794_RS02655_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064921.1"
                     /locus_tag="C794_RS02655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_017795598.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.5e-80, bitscore:
                     268.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTIVDKARNDMTAFEEMESAVRSYSRGWPVVFEKAKGYKLWDKD
                     GNEYIDFFAGAGALNYGHNPSEMQKVMIDYIQNDGVIHSLDMATTPRKKFLESFNEVIL
                     KPRNMDYKVMFPGPTGTNTVESALKIARKVTGRDTVIGFTNAFHGMTIGSLSVTGNSFK
                     RNGAGIPLNHAISMPFDQYVDGQDSIAYIERFLEDSGSGVALPAAFILETVQGEGGINA
                     ARLEWVKKIEEICRKWDILLIIDDVQAGCGRTGTFFSFEEAGINPDIICLSKSIGGVGL
                     PMAITLIKPEFDQWGPGEHNGTFRGNNLAFLAATEALNNWKTESFSQNIKKMSSLFQER
                     MKRTVEKFPQLNADLRGRGLMLGIGVHVDGLAGEICAEAFSRGLILETSGAKDEVVKFL
                     PPLIIDEDGIKKGMDILEESIQAALEK"
     aSDomain        3801..4799
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C794_RS02655_Aminotran_3.1"
                     /evalue="1.20E-79"
                     /label="C794_RS02655_Aminotran_3.1"
                     /locus_tag="C794_RS02655"
                     /protein_end="365"
                     /protein_start="32"
                     /score="259.7"
                     /tool="antismash"
                     /translation="FEKAKGYKLWDKDGNEYIDFFAGAGALNYGHNPSEMQKVMIDYIQ
                     NDGVIHSLDMATTPRKKFLESFNEVILKPRNMDYKVMFPGPTGTNTVESALKIARKVTG
                     RDTVIGFTNAFHGMTIGSLSVTGNSFKRNGAGIPLNHAISMPFDQYVDGQDSIAYIERF
                     LEDSGSGVALPAAFILETVQGEGGINAARLEWVKKIEEICRKWDILLIIDDVQAGCGRT
                     GTFFSFEEAGINPDIICLSKSIGGVGLPMAITLIKPEFDQWGPGEHNGTFRGNNLAFLA
                     ATEALNNWKTESFSQNIKKMSSLFQERMKRTVEKFPQLNADLRGRGLMLGIG"
     gene            5001..5387
                     /locus_tag="C794_RS02660"
     CDS             5001..5387
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064922.1"
                     /locus_tag="C794_RS02660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_017795599.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.4e-54, bitscore:
                     180.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKSLEDIQGTEDHQKGETWESRRFVLNKDNVGFSLNDTIIKAG
                     TESYFWYKNHIEAVYCIEGEGEVEKKDTGEVWQLKPGTMYLLNDNDKHYLRAKTQMRMV
                     CVFNPALVGTETHDKDGVYPLLAE"
     gene            5534..6145
                     /locus_tag="C794_RS02665"
     CDS             5534..6145
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795600.1"
                     /locus_tag="C794_RS02665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_017795600.1"
                     /transl_table=11
                     /translation="MAPRISEEEKEARREHLLEAALECFSAKGYYASTVDDIVRYSNLS
                     KGSVYNYFKSKEEIFIHLLKKKRQEMVEDLTIKLAKINSPLEKLKFWIREDIPFSLEKK
                     KFMRVHVEFWLYSTDSPEVQHILVERFDDMFGLTKEIIEQGQKTGEIKQDIDAEAAASM
                     FWSLHDGIWLHAVIGYDESKLEARIKEMERVLIAYLTANA"
     gene            6221..6343
                     /locus_tag="C794_RS21145"
     CDS             6221..6343
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C794_RS21145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_017795601.1"
                     /transl_table=11
                     /translation="MLKKLFRYRNQKQDIYMKNEEHLLREKLSVQYDYIRSITR"
     gene            complement(6566..7357)
                     /gene="trpA"
                     /locus_tag="C794_RS02675"
     CDS             complement(6566..7357)
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064924.1"
                     /locus_tag="C794_RS02675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="WP_017795602.1"
                     /transl_table=11
                     /translation="MGKSKLDGVLKAKKQANKPIFVPYIMAGDGGMNNLNNRIQFLEEA
                     GASAIELGLPFSDPVADGPVIQDAGQRALANKTTISSVLEELEKYKLQRNIPIVLMTYI
                     NPVWKYGFDQFARDCSKAGVDGIIFPDIPMEEEDDVAFSLTQHEIAFIRLAAMTSTEDR
                     LERIAKRSEGFLYAVSVTGTTGERAQHEKDAYHFLKRLKQVSYVPVLAGFGISTAERAR
                     ELSAACDGVVVGSKIVQLFEQGDEEAISSLIQESIGVEVIK"
     gene            complement(7357..8556)
                     /gene="trpB"
                     /locus_tag="C794_RS02680"
     CDS             complement(7357..8556)
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795603.1"
                     /locus_tag="C794_RS02680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan synthase subunit beta"
                     /protein_id="WP_017795603.1"
                     /sec_met_domain="PALP (E-value: 5e-50, bitscore: 168.4,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTYSFPDIKGKYGEFGGRFVPELLMPAVLELEKAYEDALKDEKF
                     TEELQTYLTEYIGRETPLYHAKKLTEHAGGAQIYLKREDLNHTGAHKINNTIGQALLTL
                     RMGKKKVVAETGAGQHGVATATVCSLLGLECIVFMGEEDIKRQKLNVFRMELLGAEVVS
                     VSQGSGTLKDAVNEALRYWVNNVNDTHYIIGSVVGPHPFPKIVRDFQSVIGKETKEQSM
                     KKIGSLPDAVVACVGGGSNAMGMFYPFIDDKEVTLFGVEAAGAGLDTNQHAATLTGGSP
                     GMLHGTFTYLLQNDFGQIEEAFSISAGLDYPGIGPEHSHLHQTKRVTYSSITDEEALEA
                     FRLLSKTEGIIPALESAHAVSYATKLAKEMNPEQSIVICLSGRGDKDVEQVKERLEGEQ
                     "
     gene            complement(8553..9173)
                     /locus_tag="C794_RS02685"
     CDS             complement(8553..9173)
                     /EC_number="5.3.1.24"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064926.1"
                     /locus_tag="C794_RS02685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="WP_017795604.1"
                     /transl_table=11
                     /translation="MFIKICGISSIDTALAVADANADMIGFVFAPSKRQLSIPQAEEIT
                     KKLPHTIQKVGVFVDEPLENILSIIEKLNLDIVQLHGDETIAYQKKIPIPIIKAFPATT
                     EGLQQANQSSAKYILIDSPPVQSSRGGNGVTFNWNMLKDQPFTDKLILAGGLNTDNILE
                     AIQTVRPAGVDVSSGVELNGKKDVQKINQFITKVQKERKDVLQ"
     gene            complement(9174..9956)
                     /gene="trpC"
                     /locus_tag="C794_RS02690"
     CDS             complement(9174..9956)
                     /EC_number="4.1.1.48"
                     /GO_function="GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064927.1"
                     /locus_tag="C794_RS02690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="WP_017795605.1"
                     /transl_table=11
                     /translation="MTFLAKILEEKQTEVRIRKQEKNDLGTRSIPVYSLISQINQANSL
                     SIIAEIKRASPSKGEIQMDLDPVEQAKKYEQAGASAISVLTDQNFFQGSLKDLQKVSEA
                     VSVPVLCKDFIIDEVQIDDAKDAGASIILLILAALPLERFQELYNYASNKGLEVICEVH
                     TAEELKNALTISPAIVGINNRDLKSFEVDLQTTKQLAQLVDNNTIIISESGMRTAGDAT
                     LAAEAGAKAILVGETFMKSNQLETDFNNLRVPLVERSL"
ORIGIN
        1 tagggaggat tagaatgatt gaattaactg atgtatataa acgttttgga agaaagtctg
       61 tgctaaacgg agttacgttt caagcagaaa aaagtgcaat aacttgcttg atcggtatta
      121 atggatctgg aaaaacgact atactccaat caattatgaa tcttactcca attagtaaag
      181 gaaagatcac tatcgatggg aatctgttaa ataagaatac gtatgaaaag cttacgtata
      241 ttccagatac catcacgcta ttaccaagaa tgaaaattaa ggaagcattt atttttatgg
      301 atgattttta ttcaaactgg aatcaaaagc gtgcggatga gatgttaagc ttctttaaac
      361 ttgatcgaga agaaagaatc atgaatcttt ctaaaggaaa taaatcaaag gtgaatatgc
      421 taatgggact tgcgatggac gcggattata ttcttatgga tgaacctttt tccggaatcg
      481 atatctttag cagagaacaa attactgatg tatttactag tcatctcgtt gaagacaggg
      541 gagttatcat tacaactcat gaaattaatg atattgaaca tttaattgac aaggcaattt
      601 taatagataa tggagtagtt gttaaagaat ttaatacaga gcaattgcga gaagatgaag
      661 ggaaatctgt tgttgacgta atgagagagg tgtatcgcac ttgagtaatt ttgggaaatt
      721 agtgaacttt gaatttagac gatttttacc gatattctta ccattagtag gtattttagt
      781 cttgagccaa gtaatagggg ctattgtttt ttcaatgcga tatctgagtg atatggaacg
      841 atatctgatt caaggtcagc ggactactga agatttcata ttagagtttg gaaaactgtc
      901 aatgatggaa gtggtaaatt caatttactt tatgggacca attggagtag ccgtagtttc
      961 attgcttatt taccttttat ttatttggta tagagactgg ataggaaaaa atacttttgc
     1021 gtatcgatta cttacgcttc ctacgaacag attaaatcta tttttctcta aagctagtac
     1081 aatattcatt tttatattcg gttttataac aattcaatta attatgatgc caattcttca
     1141 gtttatatta aaattatttg ttcctgttga ttatcggctg gatatggcgt taggagctat
     1201 ggtccaagcg aattattttt tgtctatact attaccacag acaattagcg gtttttttat
     1261 tcattatggt ttaggtattg tctttacaat tggaattttt acaatggttt tattagaaag
     1321 aagttatcgt ctcataggta tagggtttgc ttttctttat gcaattatag tgtgcacatt
     1381 tatatttacg ccgtattatt ttcaatactt ttttcatttg tatacacatg aagctttttg
     1441 gttagagact tctgtgatga ttataataag tgttctgtct atatggttaa gcagccattt
     1501 attaaataac aaaataaggg tgtgaataat aatgaagcat ataaaaaaat attgggggtt
     1561 atgggcaatt ataattactt ctacaatagc gatagttatc tattatgttc atatggaaac
     1621 ttctaaaatc aatctagaat ggaaaactgt atctggagaa gaaaagattg tagaggattt
     1681 aagtattaaa ggacacttat tcactccata tcaagaagat tatttttctt ctactccaaa
     1741 taattttgaa agatctagtc aagaaaatgt gttattaagt aatggtttaa gtatttatca
     1801 acatgaacgc ttaaatcgtt ttatcgacga gtaccaatca tttatgagag ggaaatcaat
     1861 ggatgatagt ttgtatcatg aaacagatga aaaaataata tatacgagtg tggacgatga
     1921 ggaagaaaat ctgttgattg aattactaga taaacaaaca aaggaagttt ataaatttga
     1981 gtatcctttt gaaaatatag agggtagtta tataagtgtt aattctactg aatatgtgga
     2041 agatgaatta tatattatta taagttctct agatattaat ggggaagctg gtgaaaatta
     2101 tgagtactat ttgtatacaa tcgatatgaa aaacaatcaa ttagtagaag agcgtaaaat
     2161 tattggtaag gaagatcaag agaaaacgga tattatccaa ttgattgatg atacatttac
     2221 ggagaaccaa aaggtagtaa tgtttgaaaa gtataatagt cctgaactta ttagttacac
     2281 taatgaaaat ggagaatatg tttctgagcg gtcaatggat gatagtgatg tagaagatgt
     2341 tagacaatta tttttattgg atttgattaa taatgaaaaa attgaaattc ctaacactat
     2401 tgcacttggg agtaatcata taatacgtaa tcaatttgtc tattacctaa gggaagaagc
     2461 tgaggctagg tacatagaac agtataatat taataagcaa gagatagata caattgcaac
     2521 tttaaatgga gaagacactg gatatggaac gttattacaa gaaaaaaatg gttatatgta
     2581 tattgcaaat cttaatagta acttagaaat caatttagaa gttattaata tagaaaacgg
     2641 agacagttct tataaaggac atattgtagt tagtgaatta tcagaacaat tacttaaaga
     2701 acaaattcaa attatgcctc aagaattgga attccgatat taaatgaaac ttattataaa
     2761 aaatgtatgt ttgcattgat acatgtgacg atacgtgttt gcaaattttc acatatatta
     2821 tattgaatct agacaagatt tgtcatagat caacttccaa caacccatga ataggagaaa
     2881 ccgcaagacg gtttctccat ttttttatga ttacataaaa ttcaactata taataaggaa
     2941 gtttctattt ggaaattttt aggttatcca aatttttaac tatttggcac gatttgggac
     3001 tatttaataa gcgatggcat ctaaacgaag ctcagtccat ttaatgggca ttaatcgcta
     3061 ttaatcacga ttaatccgtt ttgacgaatt ggtaaattat ttatattcta aaagacgtga
     3121 ttgttcaaat aatttggagg tgaaagcatc actacgattt tagatgttgc tgaaaagtta
     3181 aaggtagata cggatgagta ttactttaga agaccaacaa aagaagatgg gggtcccgtt
     3241 tgggaactag ttagagagat aggaaattta gatttaaatt catcgtatag ttatgtacta
     3301 tggtgcgaag ttttcgcaga atcttccatc gtcgtagagc ataaagaaac acaacaaatt
     3361 gtcggattta tttcgggatt tatgcatcct gaaaaggaag ataccctctt tatatggcag
     3421 gttgccgttt cttcaacgca aagaggaaaa ggattagcaa ccaagatgtt acttcaatta
     3481 cttgagtgga acgagtcggt taattttatt gaggcgacag ttgccccttc aaataaacca
     3541 tccaattact tgttcctagg attagcaaga aagattcata caaactggaa gattagcgat
     3601 tattttaaaa cagaacattt tccggctaaa gatgaagaac atgaagagga actacttttt
     3661 agaatagggc caatgagaaa aaataaaaat aaaaggatga tataatgaca acgattgttg
     3721 ataaagcccg gaatgatatg actgcatttg aagaaatgga atcagcggta agaagctata
     3781 gtcgtggctg gccggtagta tttgaaaaag cgaaaggcta taagctttgg gataaagatg
     3841 gaaacgaata tattgacttc tttgctgggg caggtgcact aaattatggt cataacccaa
     3901 gtgaaatgca aaaagtcatg attgattata tacaaaatga tggtgtcatt catagccttg
     3961 atatggctac cacaccacga aaaaaattct tagagtcatt taatgaggtt attctaaaac
     4021 cacgtaacat ggattataaa gtgatgttcc ctggccctac aggcacgaat acggttgaaa
     4081 gtgctttaaa aattgctcgt aaggtaacag gaagagatac ggttattggt tttactaatg
     4141 cgtttcacgg aatgacaatc ggttccctat cagttacagg taattccttt aaacgtaatg
     4201 gagctggaat accgttaaat catgcaattt ctatgccatt tgatcaatat gtagatgggc
     4261 aagattcaat tgcatatatt gaacgtttcc tagaggattc tggaagtgga gtagcgctac
     4321 cagctgcatt tattttagaa acagttcaag gagaaggcgg gattaatgct gctcgccttg
     4381 aatgggtgaa gaaaatcgaa gaaatttgca gaaaatggga cattctatta atcattgatg
     4441 atgtgcaggc aggatgtgga agaacgggta ctttctttag ctttgaagaa gcaggaatta
     4501 acccagatat tatttgtctg tcaaaatcaa ttggtggagt tgggttgccg atggcgatta
     4561 cattaattaa gcctgaattt gatcaatggg gaccaggtga acacaacggt acattccgtg
     4621 ggaataacct tgcattttta gcagctacag aagcattaaa taattggaaa acggaatcat
     4681 tctctcaaaa tattaagaag atgagttctc tattccaaga gagaatgaaa cgtactgtag
     4741 aaaagttccc tcagttaaat gcagatttaa gaggtagagg attaatgctc ggtatcggag
     4801 ttcatgtaga tggacttgca ggcgaaattt gcgccgaagc attttctaga gggttaattc
     4861 tagagacatc cggagctaag gatgaagttg taaaattcct tccaccatta attattgatg
     4921 aagatggtat taaaaaagga atggacatct tagaagaaag tattcaagca gcactagaga
     4981 aataaggagg agttttaata atgatcgtaa aatcattaga agatattcaa ggtacagagg
     5041 atcatcaaaa aggggaaacg tgggaaagca gacgttttgt tttaaataaa gataatgttg
     5101 gatttagcct taacgatacg attattaagg cagggacaga aagctacttc tggtacaaaa
     5161 accatattga agcagtatac tgcattgaag gtgaaggaga agtagagaaa aaggatactg
     5221 gtgaggtatg gcagttaaaa ccaggaacga tgtatctttt aaatgataat gataagcatt
     5281 atttacgtgc aaaaactcaa atgcgtatgg tatgtgtgtt caacccagcg ttagtaggaa
     5341 ctgaaacaca tgataaagat ggagtatatc cattattagc tgaataatta aagatgtaaa
     5401 gaacctgact tctataggga ggagggttct tttttgctag aaattaaaga aaaatcaaaa
     5461 gggttgtaaa ttgtttcaaa aaagactact atttatataa actatccagt cagttttata
     5521 aagaggtgca atcatggctc cacgaatttc tgaagaagaa aaagaagcac gtagagaaca
     5581 tttattggaa gcagctttag agtgtttctc tgccaaggga tactatgcat caacagttga
     5641 cgatattgtt cgatattcga atttaagtaa gggatcagtc tacaactact tcaaaagcaa
     5701 agaagaaatt tttattcatt tgctgaagaa aaaacgtcag gaaatggtag aagaccttac
     5761 tattaaatta gcaaaaatca atagtccact agaaaaactg aaattttgga tccgagaaga
     5821 tattccattt agtcttgaaa agaaaaaatt tatgcgtgta catgttgagt tttggttata
     5881 ttctacagac tcaccagaag ttcaacatat tttagttgag cggtttgacg acatgtttgg
     5941 attaacgaaa gagattattg agcaaggaca gaaaacaggg gaaatcaagc aagacattga
     6001 tgctgaagca gctgcatcca tgttttggtc gttgcatgat ggaatttggt tacacgctgt
     6061 tattggttat gatgagtcaa agctagaggc acgtattaag gaaatggaaa gagtgcttat
     6121 cgcatattta acagctaatg cctaaatttg gaatcatttt agatggttcc tcatatatta
     6181 agaaactgac cggtcatttt agaaaaggaa ggtgatagta atgttgaaga aattatttag
     6241 atatcgaaat caaaaacaag atatttatat gaaaaatgaa gagcatttat tacgcgagaa
     6301 attatccgtg caatatgact atattcgttc aataactaga taaaaagcag gagtccacga
     6361 agttaaggtg ggctcttttt aataaaaaat gtgtatcttc ccagaaagaa gatacacaga
     6421 caaaagaggt aagataaata gtatttatct tactaaaata gaaatacgta acaaaataag
     6481 gtaagataaa caaaatttat cttaccttat taaaaaggtg tactctaata ggggcataga
     6541 atccatatac aataaaatga acagattact ttattacttc tactcctata ctctcttgaa
     6601 ttagagaaga aattgcttct tcatcaccct gttcaaataa ctgaacaatt ttacttccta
     6661 cgactacgcc atcacaagca gcagatagtt ctcttgcgcg ttcagcagta gatatgccaa
     6721 atccagctaa aactggtaca tagcttactt gcttgagtct ttttaaaaag tgatacgcat
     6781 ctttctcgtg ttgagcacgt tctcctgttg taccagttac agatactgca tatagaaatc
     6841 cctctgatcg ttttgctatt ctttctagtc gatcttctgt gctcgtcatg gcagctaagc
     6901 gaataaatgc aatctcgtgc tgagttaacg aaaatgctac atcgtcttct tcttccattg
     6961 gaatatctgg aaaaattatg ccatcaacac ctgccttcga gcaatctcta gcaaattgat
     7021 cgaagccata tttccataca ggattaatgt aggtcataag aacaataggg atatttcttt
     7081 gcaatttata tttttctaat tcttctaata cagaacttat cgtagtttta tttgctagcg
     7141 cacgttgtcc agcatcttga attacaggtc catctgctac tgggtcagaa aaaggaagac
     7201 cgagttcgat ggcagaagca cctgcttctt ctaaaaactg aatacgattg tttaaattat
     7261 tcatgccacc atctccggcc atgatatagg gtacaaatat aggtttattt gcttgctttt
     7321 ttgctttcaa tacaccatct agtttacttt ttcccattat tgttccccct ctaatctttc
     7381 tttgacttgt tctacgtctt tgtcccctct accagataaa caaataacta tagactgttc
     7441 aggattcatt tccttcgcta attttgtagc ataactaact gcatgggcac tctctaatgc
     7501 agggataatg ccttctgttt tggataaaag tcgaaaagct tcaagtgctt cctcatcagt
     7561 aattgaggag tatgtcactc ttttagtctg gtgaaggtga ctatgttctg gaccgattcc
     7621 tggataatca agacctgctg aaatagagaa tgcttcttca atttgtccga aatcattttg
     7681 aagcaaatac gtaaatgtac catgtagcat acctggagaa ccaccagtta gggttgctgc
     7741 atgttgattg gtatctaatc ctgctcctgc agcttctaca ccgaataagg tcacttcctt
     7801 atcgtcgata aatgggtaga acatgcccat cgcattgctt ccaccaccaa cgcatgctac
     7861 aacagcatca ggtaaggatc ctattttctt catactttgc tctttggttt cttttccaat
     7921 cacagattga aagtcacgaa caattttggg gaaaggatga gggccgacca ccgatccaat
     7981 gatataatga gtatcattga cattatttac ccaatagcga agagcctcgt tcacagcgtc
     8041 ttttaaagtt ccactacctt gggagacact gactacttct gcccccagca attccatacg
     8101 aaagacattt agcttttgac gtttaatatc ttcttcaccc ataaaaacaa tacattctaa
     8161 tcctaataaa gaacatactg tcgcagtcgc aacaccatgt tgacctgcac cagtttcagc
     8221 cacaactttt ttcttaccca ttcttaaagt gagtagtgct tgcccaatgg tattattaat
     8281 tttatgagca ccagtatgat ttagatcttc acgttttaaa tagatttgtg caccgcccgc
     8341 atgttcagtt aactttttag cgtgatatag aggagtttca cgtccgatgt attccgttaa
     8401 atatgtctga agttcctccg tgaatttctc atctttcaac gcatcttcat aggctttttc
     8461 taattcaagt actgcaggca ttaataattc tggtacaaat cttccaccga attctccgta
     8521 ttttcctttg atgtctggaa atgaataagt agtcattgca atacatcctt tctttctttt
     8581 tgtactttag taataaattg attgatcttc tggacatctt tttttccgtt tagttctact
     8641 ccgcttgaga catcaactcc agctggtctt acagtttgga tagcttcaag tatattgtct
     8701 gtatttaaac caccagctaa tattaatttg tcagtaaaag gctgatcttt gagcatattc
     8761 caattgaatg ttacaccgtt tcctcctctt gatgattgaa cagggggact gtctattaaa
     8821 atatatttag ccgatgattg gtttgcttgt tgtaatccct ctgtagttgc tggaaatgct
     8881 ttaataatgg gaattggaat ctttttttga taggcaatag tttcatcccc atgtaactgg
     8941 acaatatcga gatttagctt ttcgattatt gatagaatat tctctaaagg ttcatctaca
     9001 aataccccca ctttttgaat ggtgtgagga agtttttttg ttatttcctc agcttgaggg
     9061 atggataatt gtcgcttact tggggcaaat acaaatccaa tcatatcagc gttagcatcc
     9121 gcaactgcta gagcagtatc aatggaagaa attccacata tttttataaa catttataaa
     9181 ctcctttcaa caaggggcac gcgtagatta ttaaaatctg tttctaattg atttgatttc
     9241 ataaacgtct ctccaactaa aatagctttc gctccagctt cggcagctag cgttgcatca
     9301 ccagctgttc tcatgccact ttcgctaatg attatagtat tattatcaac tagttgagcc
     9361 agttgtttcg tagtttgtaa atcaacttca aaggatttta aatcacggtt attaatgcca
     9421 acaatagctg gtgatatcgt taaggcgttt tttaactctt ctgcagtatg cacttcacaa
     9481 atgacctcaa gtcctttatt tgaagcataa ttatataatt cctggaatct ttctaatggt
     9541 aaagctgcta gaattaacag gataatagaa gcccctgcat cttttgcatc atcaatttga
     9601 acctcatcaa taataaaatc tttacaaaga actggaaccg aaactgcttc acttactttt
     9661 tgtaaatcct ttaatgaacc ttgaaaaaaa ttttgatctg ttaatacaga tatggcagat
     9721 gcacctgctt gctcgtattt tttagcttgc tcgacgggat caaggtccat ttgaatttct
     9781 ccttttgaag gagaagcgcg tttaatttca gcaataatgg atagtgagtt tgcttggttg
     9841 atttgactta tcaacgaata aacaggtata cttcgggtcc ctaaatcgtt tttctcttgt
     9901 tttctaatcc ttacttcagt ttgtttttct tctaagattt ttgctagaaa agtcatctaa
     9961 accacctcgc taactttctt actgaaagtg attaggttat tgagtttgct taatgctgaa
    10021 ccagaattaa tactttctct agcaagttta acgccatcat aaatggaacc cactcttctt
    10081 gtagtaaaca atgcaatgcc tgcattgaaa agtacggtat ccagatacgc gctttcttca
    10141 cctcttaata tagacagtaa gatatttgcg ttatctgttg catttcctcc ttgaatttca
    10201 tcattcggat atacagataa accaacctct tctggatgaa ttgttctttc ctctaatcgg
    10261 tcattttcaa cgagtatata ttgattttct ccttgtaaag aagcctcgtc taaaccacca
    10321 gcaccatgga tcacaattgc tcgttctctt ccaagtagtt gtgcagcttt tgccaccatt
    10381 ggaatag
//
