LOCUS       NC_019673              10390 bp    DNA     circular CON 12-DEC-2024
DEFINITION  Saccharothrix espanaensis DSM 44229, complete sequence.
ACCESSION   NC_019673
VERSION     NC_019673.1
KEYWORDS    RefSeq; complete genome.
SOURCE      Saccharothrix espanaensis DSM 44229
  ORGANISM  Saccharothrix espanaensis DSM 44229
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Saccharothrix.
REFERENCE   1
  AUTHORS   Strobel,T., Al-Dilaimi,A., Blom,J., Gessner,A., Kalinowski,J.,
            Luzhetska,M., Puhler,A., Szczepanowski,R., Bechthold,A. and
            Ruckert,C.
  TITLE     Complete genome sequence of Saccharothrix espanaensis DSM 44229(T)
            and comparison to the other completely sequenced Pseudonocardiaceae
  JOURNAL   BMC Genomics 13, 465 (2012)
   PUBMED   22958348
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360653)
  AUTHORS   Rueckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2012) IGS, CeBiTec, Bielefeld University,
            Universitaetsstr. 27, D-33335, GERMANY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000328705.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:06:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,523
            CDSs (total)                      :: 8,451
            Genes (coding)                    :: 8,313
            CDSs (with protein)               :: 8,313
            Genes (RNA)                       :: 72
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 43 of 138
            Pseudo Genes (incomplete)         :: 116 of 138
            Pseudo Genes (internal stop)      :: 7 of 138
            Pseudo Genes (multiple problems)  :: 27 of 138
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:45:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4911670
            Orig. end                         :: 4922060
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HE804045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="20"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(1058..1450)
                     /locus_tag="BN6_RS21775"
                     /old_locus_tag="BN6_44970"
     CDS             complement(1058..1450)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN6_RS21775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BN6_44970"
                     /product="hypothetical protein"
                     /protein_id="WP_015101889.1"
                     /transl_table=11
                     /translation="MTYDIAAFESADTDDDGWEAWYGDQHDYFDAHPGADVSVVSPKLQ
                     AFYRDLAVTVSAAERPEDGETSAEFDELVDTDPMAAIFTFGPRIVYGEHSHGQERQAVD
                     LWLALAEKHGLAVALLGESNLPIHRF"
     misc_feature    complement(1516..1531)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     misc_feature    complement(1534..1549)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.51"
                     /tool="antismash"
     misc_feature    complement(1598..1611)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.52"
                     /tool="antismash"
     gene            1605..1994
                     /locus_tag="BN6_RS21780"
                     /old_locus_tag="BN6_44980"
     CDS             1605..1994
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101890.1"
                     /locus_tag="BN6_RS21780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_44980"
                     /product="hypothetical protein"
                     /protein_id="WP_015101890.1"
                     /transl_table=11
                     /translation="MIFYTDAESRTLRKAVFGVMVLVSHADPGPVLEERYAGLRALTHL
                     SPELRGVLHASRPDLPPVPDAELEEQVVQALVASLKILTAKAPADAATFPAAVEAICHE
                     VAAADGQVAEVEAAVTAKVRAALNG"
     gene            complement(2074..3066)
                     /locus_tag="BN6_RS21785"
                     /old_locus_tag="BN6_44990"
     CDS             complement(2074..3066)
                     /EC_number="1.8.5.-"
                     /GO_function="GO:0004364 - glutathione transferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101891.1"
                     /locus_tag="BN6_RS21785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_44990"
                     /product="glutathione S-transferase family protein"
                     /protein_id="WP_197540368.1"
                     /sec_met_domain="GST_C (E-value: 1.5e-06, bitscore: 27.3,
                     seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGKEFTRDSRYITTRITTDVRDGFPVEPGRYRLVAARACPWANR
                     AIIVRRLLGLEDAISLGLPGPTHDARSWTFDLDPGGRDPVLGIERLQEAYFARDPEYPR
                     GITVPAIVDTATGAVVTNDFAQITLDLSTQWRAFHREGAPDLLPAAHRDEIDEVNRRVF
                     TEVNNGVYRCGFAGSQEAYDEAYDRLWTALDWLEERLADRRYLVGDTITEADVRLFTTL
                     VRFDPVYHGHFKCNRNKLSEQPNLWAYARDLFQTPGFGDTVDFVQIKQHYYVVHTDINP
                     SRIVPKGPDLSGWVTPHGREALGGRPFGDGTPPGPVREGERVDPAHGPR"
     misc_feature    complement(3098..3113)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.78"
                     /tool="antismash"
     gene            complement(3207..3587)
                     /locus_tag="BN6_RS21790"
                     /old_locus_tag="BN6_45000"
     CDS             complement(3207..3587)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN6_RS21790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BN6_45000"
                     /product="hypothetical protein"
                     /protein_id="WP_148302959.1"
                     /transl_table=11
                     /translation="MNRRVRLLATLLAAFAALFTVSAATAQAAPDPDIFVFSAPQGPCE
                     TAGGNGVTGGVFADYACNPIFIDYGLFVEPTYGSFDDVYIATFADEPTCEAAGDNGVNG
                     GVWADYQCVIGTASGYLLLVSN"
     gene            complement(3670..4044)
                     /locus_tag="BN6_RS21795"
                     /old_locus_tag="BN6_45010"
     CDS             complement(3670..4044)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN6_RS21795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BN6_45010"
                     /product="hypothetical protein"
                     /protein_id="WP_015101893.1"
                     /transl_table=11
                     /translation="MTKLARVLGAVVTASAATLALTGGIAQAAPNTVHLYTGTEALCQK
                     IGDGGVANDVFDSYECKSGFGGYSTLVDPSPDLLAGVPVFTDAEAVCETVGTNGVTNGA
                     WSAYYCQFGIVGWTLLVDRW"
     misc_feature    complement(4178..4195)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.09"
                     /tool="antismash"
     misc_feature    4225..4240
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.39"
                     /tool="antismash"
     misc_feature    4878..4893
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.76"
                     /tool="antismash"
     misc_feature    4885..4898
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.3"
                     /tool="antismash"
     gene            5001..5390
                     /locus_tag="BN6_RS42210"
                     /old_locus_tag="BN6_45020"
     CDS             5001..5390
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.5"
                     /locus_tag="BN6_RS42210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_45020"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_051075675.1"
                     /sec_met_domain="melC (E-value: 4.7e-27, bitscore: 93.7,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHVHRDGSDKAVGRRRFLVGGLVGGGVAVAAGAGLIGLGSSAQAV
                     TLEEFDEIYEGRRIKGWAVKDADDPRSEATVDGDALHVMRNGDGTFTTSVNHYESFDSL
                     KTATRHAVDTLGGLRPETQLAHHGR"
     gene            5416..6234
                     /locus_tag="BN6_RS21805"
                     /old_locus_tag="BN6_45030"
     CDS             5416..6234
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101895.1"
                     /locus_tag="BN6_RS21805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_45030"
                     /product="tyrosinase family protein"
                     /protein_id="WP_015101895.1"
                     /transl_table=11
                     /translation="MGIRKNQQNLTATERSNFVNALLTLKQNGRYDLFVRLHIDRANGD
                     SDWGLRIGHRSPSFLPWHRRYLIEFEQALQEVNPSVTIPYWDWTTAGSTSTLWANDFLG
                     PNGTSATNWRVNAGPFAQSTGQWTLTVRSDSANYLRRNFGSGGLQLPSASDVNSVLGLT
                     TYDTSPWNSTSINSFRNYLEGFRGPNLHNRVHNWVNGTMAGFGSPNDPVFWLHHCNVDR
                     LWSQWQAQHPGSTYLPTAGTANVVDLNEAMNPWTTTPNDMLNHSAVYTYQ"
     gene            complement(6352..7287)
                     /locus_tag="BN6_RS21810"
                     /old_locus_tag="BN6_45040"
     CDS             complement(6352..7287)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024206.5"
                     /locus_tag="BN6_RS21810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_45040"
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="WP_041313584.1"
                     /transl_table=11
                     /translation="MVDLLPEARELDSIRSDPAEAARYGFGSVAELALAVGVKRAIADG
                     DVERLRGLVAGRPEVAGGGMSSRFAGSTGACLLNYLGAARFHGILDHDRVGGVARVLLA
                     AGAPADGPADVRETPLITAASYGEVELARVLVDAGADLRAVGYAVAGGTALAHAVEYGN
                     TDVADLLVAAGAEPAGVVEAAGAGDLGPVREQWSDATAAQRVLALRAAAVNGRSAVVDW
                     VVSNGVAVDDSVDGSGQTALHWAAWHGKPESVAHLLALGADPGRTEAEFGGTPLAWCRH
                     RRAQVEVYRASAGHAAVEAVLLSLADPDVT"
     gene            complement(7427..9043)
                     /locus_tag="BN6_RS47975"
                     /old_locus_tag="BN6_45050"
     CDS             complement(7427..9043)
                     /codon_start=1
                     /function="Subtilisin-like serine proteases"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.5"
                     /locus_tag="BN6_RS47975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_45050"
                     /product="putative Ig domain-containing protein"
                     /protein_id="WP_015101897.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.2e-14, bitscore:
                     52.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKKSLSLLTVALVLSAGNVALSAPAAAAAPAGPGAVLEVKLAAG
                     VGVAAVGQVLDEREIASVRPLFSRLDEKLAALRPVGAKAAQAPGLERWQRVRLAPGVDA
                     KAAARELTASGRVEVAYPQPEGSDPVTPNFAGQQVYADPVTSGGIDADYAHTKPGGKGG
                     NVKVVDLERNWNSQHEDLSKLRLPGALIANGTPDFTPTSIDHGTAVTGVIGADANTFGV
                     TGLVPEAGLHYTNVVSQENGYDLANALLTAGAGVGVGDVLLIEQHVTYCADWAPMEVWD
                     SVYDAIVTVVQSGRHVVEAGGNGNQDLNNACFGPRFPADKPDSGAIIVGAGAAPGCTGT
                     PRSKLGFSNYGTRVDLQGWGECVTTTGYGDLHGTGANDKYTAYFSGTSSASPVVASALA
                     SLLSVAEANGETLSPAEAREILIATGTAQSGTQHIGPLPNLLTAVGNYLANVNFPERVA
                     YPGPRQATRTVPTSLALQAWDGDDDPLTFWATGLPPGLTLAPATGVISGTPTTAGTYTV
                     TVYASDSYTGPAQTTFTWTVS"
     misc_feature    9240..9255
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.69"
                     /tool="antismash"
     misc_feature    9397..9412
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.5"
                     /tool="antismash"
     misc_feature    10089..10104
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    10098..10111
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 cggatgctgc ccgccctggg cctggtgacg ctggcgagcc tcgcgtggct ggtgccgctc
       61 aagtcggccg gcgcccaacg ctggtacctg ctggccgtcg tgctcggggc gacggtggcc
      121 acgtgggtcg cgttgcgcct ggtcgtccgc cggctgcccc gcttgtcccg ccccgcgcgc
      181 gtgctggtcc tcgtggtgta cgcggtgatg ccggtcgccg tcccgctgct ccagctcgtc
      241 gtcgacgaca ccgtgacacc gccggccggc agcgcggtgg gcctgctggg gttcgtgatc
      301 ttcttcgccg ccgtgttcgc gggcacgctg ctggccacca cgtacggcct gggccggctg
      361 ctgcgccggg ccgtccggca caccgtctac gacctgcgca acagcgtgca gctgctgggc
      421 cgcgcgctgc cggcgatgct gttcgtgacc ctcttcctgt tcttcaccgg cgagctgtgg
      481 caggccatga acaacttcgc gtggtggcgg ctcaccctcg tggtggtgct cttcggcgcg
      541 atcacggtgc tggccgccgc cgcgcgcctg cgcgacgaga ccggccgggt ggagcaggag
      601 ctgagcccgg agcggctgac cgccgcctgc ctgggcacgc cgctggcggg cgtcgcgatc
      661 accgaacccc tgcgccccac cccgttgacc ggccggcaga accgcaacct gctgctcatg
      721 cttgcgaccc gccagctcgt gcaggccgcg ctggtggggc tcgcgctgtt cgcgttcttc
      781 ctggtgctgg gcctgctggt ggtcacaccg gagaccgccg agcagtggat cggctcgccg
      841 ccgaactact ccaagtggct gccggcgatc ccgacggcca tgctgcgcaa cgcgacgttg
      901 ttcgccgcgt tcggcagcat gtacttcgcg atcacctcga tgagcgacgc ggaccaccgc
      961 aagcagttct tcgcgccgat catcgacgag gtggagcgca cgctggccgt ccgcgccgtc
     1021 tacctgtccc tgcgcgaccg ggcgtgaccc tggagcgtca gaaccggtgg atcggcaggt
     1081 tggactcgcc cagcagcgcc acggccagcc cgtgtttctc ggccagcgcc aaccacaggt
     1141 cgacggcctg ccgctcctga ccgtgcgagt gctcgccgta cacgatccgg gggccgaagg
     1201 tgaagatcgc cgccatcggg tcggtgtcca ccagctcgtc gaactccgcg ctggtctcgc
     1261 cgtcctcggg ccgttcggcg gcgctgacgg tcaccgccag gtcccggtag aacgcctgga
     1321 gcttcggcga cacgaccgac acgtccgccc cggggtgggc gtcgaagtag tcgtgctggt
     1381 cgccgtacca ggcttcccag ccgtcgtcgt cggtgtcggc cgactcgaac gcggcgatgt
     1441 cgtaggtcat ggacctcgga cccgggccgc cgccgacccg ttcccgacgc ccggcccgaa
     1501 cccgcttccc ggcgtcgtga cgcgcgtcac ccctgatcgc tcccgccgac gagggcggcc
     1561 ggggcaccgc gaggggccga cgagatcgtc cttaggctga acgcatgatc ttctacaccg
     1621 atgccgagag ccggaccctg cgcaaggccg tcttcggcgt gatggtgctg gtgtcgcacg
     1681 ccgaccccgg ccccgtgctg gaggagcgct acgccggtct gcgggccctc acccacctgt
     1741 cgccggagtt gcgcggcgtg ctgcacgcct cccgccccga cctccccccg gtccccgacg
     1801 ccgaactgga ggagcaggtc gtgcaggcgc tggtggcgtc gctgaagatc ctcaccgcca
     1861 aggcccccgc ggacgccgcc acgttcccgg ccgccgtcga ggcgatctgc cacgaggtgg
     1921 ccgccgccga cggccaggtg gccgaggtcg aggccgcggt gaccgccaag gtgcgcgccg
     1981 ccctcaacgg ctgacgccgc gcgcccgcac ctccggcagg cccgcacctc cggcaggccc
     2041 gcaccccagg tcggcgcggg ccgtgcgccg gggtcagcgc gggccgtgcg cggggtcgac
     2101 gcgttcgccc tcgcggaccg gtcccggcgg ggtgccgtcg ccgaagggcc gcccgcccag
     2161 cgcctcgcgg ccgtgcggcg tcacccagcc cgacaggtcc gggcccttgg gcacgatccg
     2221 cgacgggttg atgtcggtgt gcacgacgta gtagtgctgc ttgatctgga cgaagtcgac
     2281 cgtgtcgccg aaaccgggtg tctggaacag gtcgcgggcg taggcccaca ggttcggctg
     2341 ctcgctgagc ttgttccggt tgcacttgaa gtgcccgtgg taaaccgggt cgaagcggac
     2401 cagcgtggtg aacagccgca cgtccgcctc ggtgatcgtg tcgccgacca ggtagcggcg
     2461 gtcggcgagg cgctcctcca gccagtccaa ggcggtccac agccggtcgt acgcctcgtc
     2521 gtaggcctcc tggctgcccg cgaagccgca ccggtagacg ccgttgttga cctcggtgaa
     2581 gacccgccgg ttcacctcgt cgatctcgtc gcggtgcgcg gcgggcagca ggtccggcgc
     2641 gccctcgcgg tggaacgccc gccactgcgt cgacaggtcg agggtgatct gcgcgaagtc
     2701 gttggtgacc accgcgccgg tcgcggtgtc cacgatcgcg ggcacggtga tgccgcgcgg
     2761 gtactccggg tcgcgggcga agtacgcctc ctggaggcgc tcgatgccca gcaccgggtc
     2821 ccggccgccc gggtcgaggt cgaaggtcca cgaccgggcg tcgtgcgtcg ggccgggcag
     2881 gcccagcgag atcgcgtcct ccagccccag cagccggcgc acgatgatcg cccggttcgc
     2941 ccacgggcag gcccgcgccg cgaccagccg gtaccggccg ggctccaccg ggaagccgtc
     3001 ccggacgtcg gtcgtgatcc gggtcgtgat gtaccgggag tccctggtga actccttgcc
     3061 cttcaccgaa taggtcccgc ctgtgctgtg ctccgggtgc tcgctcacac cgtcgagcct
     3121 acgacgttgt cgccgcgccg cagcccgacc gccgggcggc cgcccgccgc cgaggtcggc
     3181 gggcgggcgg ccggtggtcg gcgaggtcag ttgctgacca gcagcaggta cccggaggcc
     3241 gtgccgatga cgcactggta gtcggcccag acgccgccgt tcacgccgtt gtcgcccgcc
     3301 gcctcgcacg tgggctcgtc ggcgaaggtg gcgatgtaga cgtcgtcgaa cgagccgtag
     3361 gtcggctcca cgaacaggcc gtagtcgatg aagatcgggt tgcacgcgta gtcggcgaac
     3421 accccgccgg tcaccccgtt gcccccggcg gtctcgcacg ggccctgcgg ggccgagaac
     3481 acgaagatgt ccgggtccgg cgcggcctgg gccgtcgcgg cggagaccgt gaagagggcg
     3541 gcgaaggccg cgagaagggt cgcgagaagg cgcactcgtc ggttcatgtg gattcctcca
     3601 gcgtctttgc gggatcggcg taacacgaac gagcgggtgg cggcccgggg tcccggacgg
     3661 ccaccggagt caccagcggt cgacgagcag ggtccagccg acgatgccga actggcagta
     3721 gtaggcgctc cacgcgccgt tggtcacgcc gttggtcccg acggtctcgc agacggcctc
     3781 cgcgtcggtg aagaccggga cacccgcgag caggtcgggg ctcgggtcga ccagcgtgct
     3841 gtagccgccg aatccgctct tgcactcgta gctgtcgaac acgtcgttgg cgacgccacc
     3901 gtcgccgatt ttctggcaga gcgcctcggt gccggtgtag aggtggacgg tgttgggggc
     3961 ggcctgcgcg atccctccgg tgagcgcgag ggtggccgcg ctcgcggtca ccacggcgcc
     4021 cagcacgcgc gcgagctttg tcatgtcgac tctcccacaa tgtcggattc gacggaccgg
     4081 cgccgcgggg cagcacctga accgacgtta acagcggatt accggtaacc gtccagccat
     4141 tcttgtcgac tgtgaaacac ccactgcgga atcaactgca acaccaccgg aacatgctgg
     4201 tgacagtcct cgtagccagg catatgtgag ctgcgtcacg caccgtagta acagtttaga
     4261 cagtcacttg cgggggtaca ccggccagca ttcgcaggac gcggtggacc atctgtcgca
     4321 acgctgtgac acaaccggac ggtgccgaca ccgcgcattc gcgagacatc caggtcagcg
     4381 ccccccaggc cgcccagtct ccctacgtca gcggcggtcg gacctgctcc gacgtgcccg
     4441 aaccggcggg ccggccccac caggcccgcc ggcgctcgac ccacctcggg cggggtgttc
     4501 gcgctcattc cgcccacccg gtccgcacga cgccccgccg gccggggcgg aaagcaggcg
     4561 caccgcgcac cctgtccttt cggcttggtg cagccggccg gacggacagg ttcccggaat
     4621 accggagaac cgatccgtgc gggtttccga actcagtggt tccgcaggcg gcacgcggtg
     4681 gtgcgcattc taccgagccg cgtcgcagca gtggcgggcg ccgtccgaat accggacgca
     4741 agactgcttg acagccgtcg agaactgccg atgaataacg atttgacgcc ccgcgcgcac
     4801 ttctaccgac ggatccggac gacgacaagg tgatcgccac cacctgttta acactttacc
     4861 gccggcagat cgcctagttg gcggctcttt cacagaaatg tccgctgata atgtcgggac
     4921 cgcggtgctt ccagggggag ctgaaggccg cccttcggat tgttcgcgcc gcgttcacca
     4981 cacagacagg tggcacacac atgcatgtcc acagggacgg ttccgacaag gcggtcggcc
     5041 ggagacgatt cctcgtcggc ggtctggtcg gcggtggcgt cgcggtcgcc gccggcgccg
     5101 ggctgatcgg gctgggttcc agcgcgcagg ccgtgacgct ggaggagttc gacgagatct
     5161 acgagggccg gcggatcaag gggtgggcgg tcaaggacgc cgacgacccg cgcagcgagg
     5221 cgaccgtcga cggcgacgcg ctgcacgtca tgcgcaacgg cgacggcacg ttcaccacgt
     5281 cggtgaacca ctacgagtcg ttcgactccc tcaagaccgc gacccggcac gccgtggaca
     5341 ccctcggcgg gctgaggccg gaaacccaac tggcccacca cggccgttga tccgggtaca
     5401 ggacaggggg agatcatggg gatccgcaag aaccagcaga acctgaccgc caccgagcgg
     5461 tccaacttcg tcaacgcgtt gctcacgctc aagcagaacg gccgctacga cctgttcgtg
     5521 cggctgcaca tcgaccgggc caacggcgac agcgactggg ggctgcggat cgggcaccgc
     5581 tcgccgtcgt tcctgccgtg gcaccgccgg tacctgatcg agttcgagca ggcactccag
     5641 gaggtcaacc cgtcggtcac catcccgtac tgggactgga cgaccgccgg cagcacgtcc
     5701 acgctgtggg ccaacgactt cttgggcccg aacgggacgt cggcgaccaa ctggcgggtc
     5761 aacgccggcc cgttcgccca gtccaccggc cagtggacgc tcaccgtgcg cagcgactcg
     5821 gccaactacc tgcgccgcaa cttcggctcc ggcgggctgc aactgccctc ggcgagcgac
     5881 gtgaactccg tcctggggct gacgacctac gacacctcgc cgtggaacag cacctcgatc
     5941 aacagcttcc gcaactacct ggagggcttc cgcggcccga acctgcacaa ccgggtgcac
     6001 aactgggtca acggcacgat ggcgggcttc ggctcgccca acgaccccgt cttctggctg
     6061 caccactgca acgtcgaccg cctgtggtcg cagtggcagg cccagcaccc cgggagcacc
     6121 tacctgccca ccgcggggac ggcgaacgtg gtggacctca acgaggcgat gaacccgtgg
     6181 accaccacgc cgaacgacat gctcaaccac agcgcggtgt acacctacca gtgacggccg
     6241 cgcccccgac cgggggcggc cgccgctgac cacgtcgatg acctcgcgac cacccggtcg
     6301 cgaggtcatc gacgcacccg cgaccatcca caacggacag acgcgggctc atcacgtcac
     6361 gtcgggatcc gccagggaga gcaacacggc ctccaccgca gcgtggccgg cggacgcccg
     6421 gtagacctcg acctgggcac gccggtgccg gcaccacgcg agcggggtgc cgccgaactc
     6481 cgcttcggtg cgaccggggt cggcgccgag cgccagcagg tgggcgacgg actccggctt
     6541 gccgtgccag gccgcccagt gcagcgcggt ctgccccgac ccgtccaccg agtcgtccac
     6601 ggccaccccg ttcgacacca cccagtcgac caccgccgac cgcccgttca ccgcggccgc
     6661 ccgcagtgcg aggacccgct gcgccgccgt cgcgtcagac cactgctcgc gcaccggccc
     6721 gaggtccccc gcaccggccg cctcgaccac cccggccggc tccgcgcccg ccgccaccag
     6781 caggtcggcc acgtcggtgt tgccgtactc gacggcgtgc gccaacgccg tgccgcccgc
     6841 gacggcgtac ccgaccgccc gcaggtccgc gcccgcgtcg accagcaccc gcgccagctc
     6901 gacctcgccg tacgacgcgg cggtgatcag cggcgtctcc cgcacgtcgg ccggcccgtc
     6961 cgccggcgcg ccggcggcga gcagcacgcg ggccaccccg ccgacccggt cgtggtcgag
     7021 gatcccgtgg aaccgggccg ccccgaggta gttcagcagg caggccccgg tgctcccggc
     7081 gaacctcgaa ctcatcccgc cgccggccac ctcgggccgt ccggcgacca gaccgcgcaa
     7141 ccgctcgacg tccccgtccg cgatcgcacg cttcaccccg accgccagcg cgagttcggc
     7201 gaccgacccg aacccgtacc gggcggcctc ggccgggtcg gaccggatcg agtccagctc
     7261 acgggcttcc ggcaacaggt ccaccatcgg gcgctcctcg ggtcgaccgc gtcctgcgac
     7321 acggggttcc attgtcccga caaaccgctg cggcgggcgc ggaccattcg cacccaccgc
     7381 agcggtcact gcccgggacc tcccggtcgg acgactcgcc cgacgcctag gagacggtcc
     7441 aggtgaacgt cgtctgcgcg ggcccggtgt agctgtcgct ggcgtacacg gtgaccgtgt
     7501 aggtgccggc ggtggtcggg gtccccgaga tcacgccggt cgccggcgcg agggtcagcc
     7561 ccggcggcag gccggtcgcc cagaacgtca gcgggtcgtc gtcgccgtcc cacgcctgga
     7621 gcgccaggct ggtcggcacc gtccgggtcg cctggcgcgg gcccgggtag gcgacccgct
     7681 ccgggaagtt cacgttcgcc aggtagttgc cgaccgcggt cagcaggttg ggcagcgggc
     7741 cgatgtgctg ggtgccgctc tgcgccgtgc cggtcgcgat caggatctcc cgggcctcgg
     7801 cgggcgagag cgtctcgccg ttcgcctcgg ccacgctgag cagcgacgcc aacgccgacg
     7861 cgacgaccgg cgacgcgctc gacgtgccgg agaagtacgc ggtgtacttg tcgttcgcac
     7921 cggtgccgtg caggtcgccg tagccggtgg tggtgacgca ctcgccccag ccctgcaggt
     7981 cgaccctggt gccgtagttg gagaagccca gcttcgagcg gggcgtgccg gtgcagcccg
     8041 gggccgcgcc cgcgccgacg atgatcgcgc cggagtccgg cttgtcggcc gggaagcgcg
     8101 ggccgaagca cgcgttgttg aggtcctggt tgccgttgcc gccggcctcc acgacgtgcc
     8161 gcccggactg cacgacggtg acgatcgcgt cgtacaccga gtcccacacc tccatgggtg
     8221 cccagtcggc gcagtaggtg acgtgctgct cgatcagcag gacgtcaccg acgccgacgc
     8281 ccgcgccggc cgtgagcagc gcgttggcca ggtcgtagcc gttctcctgg ctgaccacgt
     8341 tggtgtagtg caggcccgct tcgggcacca ggccggtcac gccgaaggtg ttggcgtccg
     8401 cgccgatcac gccggtgacc gcggtgccgt ggtcgatgct ggtcggcgtg aagtccggcg
     8461 tgccgttggc gatcagcgcc ccgggcagcc gcagcttcga caggtcctcg tgctggctgt
     8521 tccagttgcg ctccaggtcg acgaccttga cgttgccgcc cttgccgccc ggcttggtgt
     8581 gcgcgtagtc cgcgtcgatc ccgccgctgg tgaccgggtc ggcgtagacc tgctggccgg
     8641 cgaagttcgg cgtgaccggg tcactgccct ccggctgcgg gtaggccacc tcgacccggc
     8701 cggacgcggt gagctcccgc gcggcggcct tggcgtcgac accgggcgcg agccgcaccc
     8761 gctgccaccg ctccaacccc ggtgcctgcg ccgctttggc acccaccggc cgcagcgcgg
     8821 ccaatttctc gtccagccgg gagaacagcg gccgcaccga cgcgatctcg cgctcgtcca
     8881 acacctgccc caccgcggcg acgccgaccc cggcggccag cttcacctcc aacaccgcgc
     8941 ccggtccggc cggcgccgcg gcggcggcgg gcgcggacag ggcgacgttc ccggcgctca
     9001 gcacgagcgc gacggtcagc agtgacaacg acttcttccg cacagagttg cccccatctc
     9061 agaaaaccac cggtgaggac gggcggaaac cacggcggag cgccatcgac gcgcgcggaa
     9121 ccacaccgcc ggtcaaggcg gtgcacgatc ccgatccccc gtgtatgtcc cccgttaacg
     9181 ccccagtggt tccacttcag cgcacatcgc cgtcgatgtc cagcgccggg gatcacccaa
     9241 ctcgtccaga ccaccggact cggctacgcg ttaccgaact ccccgcgccg gccgacgcct
     9301 ttccttgcgc cgtggggaac ttgcgcgcgc accggggttc aggactcgtt gtaccgggcc
     9361 tcgtgccgcc ggatcaccgg gtcggtgccg accacgttga cggtgtggtc cgcaaccctt
     9421 cccgcagcgg acaccaagtc gccgacccgg gacccgcgca ccggcccgct ggagcaggac
     9481 cggtgaccac gacgtgggca gtaagccgct cccgtgcggg gactcacccg tccagcggct
     9541 ccgctgtgcg cactggggtg atggtggcgg tgagcgggcc gtcggggcgg acggtgacgc
     9601 cgaacgtcgg cgcgggggtg gtgtgcggaa cgacggtgaa cgcgggcagg agggtctcca
     9661 gcagcagggt catctcgcgc aggccgaact ggacgcccag gcaggtccgg ggacccaggc
     9721 cgaacgggta gtacgcgccg ggggccgggc ggggcgcggt ggcggcgaag cggtccgggc
     9781 ggaagcgctc ggggtcgggc cacgcggtgt cgtcgcggtg ggtcaggtag gggctggtga
     9841 ggatggtggt gccggcctcg acgcggtagc cggccaggac gtcctcgcgc tcggcgtggc
     9901 ggggcatgat ccacgccgcc gggtagagcc gcagcgtctc gctgatcagg gcgcggatcg
     9961 cggtgccgcg ctcggccgag ccgggtggcg ttgccagggc acggtcgcgt tcggcggggt
    10021 tctcgtgcag cagcaggaac agccagctca acgtggtggc ggtggtctcg taaccggccg
    10081 ccagcagggt caccagctcg tcccggatca gccgctcggt gtactcgggg tgctcctcgg
    10141 ccgccgcgag caggacgtgc agcaggctcg ggccgggcca ctcgcgggcg gcggcgatgg
    10201 cacgggcggc gacggcgtcg atctgggcga gctcggcggt gaggtcgggc ggggcctcgc
    10261 cggtggcggg cgcgaacgcg ggcagcgccg cgaccatccg cccggcggcc aggaactccc
    10321 ggtgggtggt cgggtccagc ggccggccgg tcatcgaccg ccagatcacg tcgagcgaga
    10381 agtgccgcat
//
