LOCUS       NC_019673              10792 bp    DNA     circular CON 12-DEC-2024
DEFINITION  Saccharothrix espanaensis DSM 44229, complete sequence.
ACCESSION   NC_019673
VERSION     NC_019673.1
KEYWORDS    RefSeq; complete genome.
SOURCE      Saccharothrix espanaensis DSM 44229
  ORGANISM  Saccharothrix espanaensis DSM 44229
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Saccharothrix.
REFERENCE   1
  AUTHORS   Strobel,T., Al-Dilaimi,A., Blom,J., Gessner,A., Kalinowski,J.,
            Luzhetska,M., Puhler,A., Szczepanowski,R., Bechthold,A. and
            Ruckert,C.
  TITLE     Complete genome sequence of Saccharothrix espanaensis DSM 44229(T)
            and comparison to the other completely sequenced Pseudonocardiaceae
  JOURNAL   BMC Genomics 13, 465 (2012)
   PUBMED   22958348
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360653)
  AUTHORS   Rueckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2012) IGS, CeBiTec, Bielefeld University,
            Universitaetsstr. 27, D-33335, GERMANY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000328705.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:06:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,523
            CDSs (total)                      :: 8,451
            Genes (coding)                    :: 8,313
            CDSs (with protein)               :: 8,313
            Genes (RNA)                       :: 72
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 43 of 138
            Pseudo Genes (incomplete)         :: 116 of 138
            Pseudo Genes (internal stop)      :: 7 of 138
            Pseudo Genes (multiple problems)  :: 27 of 138
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:45:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1932881
            Orig. end                         :: 1943673
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HE804045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10792
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10792
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10792
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5792](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5792)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    563..581
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.43"
                     /tool="antismash"
     gene            complement(823..3990)
                     /locus_tag="BN6_RS09165"
                     /old_locus_tag="BN6_18660"
     CDS             complement(823..3990)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099299.1"
                     /locus_tag="BN6_RS09165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18660"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_015099299.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 5.1e-53, bitscore:
                     179.2, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPPRGRGALCAALALFGLVTAATPAGGRSPAGEVAAGARSITLV
                     TGDRVVLTGDQVGSLTGGPGRERAVFHTFRRDGHLHVVPRDAAHDLARGRLDPRLFDVT
                     ALLEAGYDRRPTIPLIVVGDPGLPRTKTLDAVGAVAVEVPKGGAADAWRTLVSRASVRK
                     VWLDGLRRPSLDRSTAQIGAPAAWSAGYTGEGVKVAVLDTGIDDGHPDLAGRVAARSNF
                     TTDPDNTDRVGHGTHVAAAIGSRHAVYRGVAPDAELLDGKVCVAAGCPESAILAGVQWA
                     VGQGADVVNLSLGGPDTPETDPLEAAVEALSARALFVVAAGNSGRPGTVSSPGSADAAL
                     TVGAVDRAEGIAEFSSRGPRPDGGVKPDVTAPGVDIAAAKAEHGTIGTPVDATHVALSG
                     TSMATPHVAGAAALLAQQHPDWSGARLKAALVASARPNPALTAFDQGAGRVDLAAAITT
                     TVTADPANVALGLQKWPHDDDTPVTKNVVYRNTGTAPVTLRPSAVVTAPDGKPSDVVTV
                     SPAEVTVPPGGDATIAVTADTRRAPVDGVYAGTIVATGGATPLRVPVSVHREVESYDVT
                     FRFVDAAGNPAKNYYASVIGMDNSTFVFLFSADGKVSLRVSKGDYFVGADVTTDRKVAL
                     LPRPDLSVTADTTVVVDARTARPVRITAPDPKAREVLGDLTTARRRGDRSASVSTAFLG
                     GFGTDVSIGHNGPDLPGEELTALLGAQLSGTPAGPTPVTYRFAWALHGRLPTGFARAVP
                     AEDLAEVRTSYGAGPAGSTYGHVGNPAAPDGTTGWGWMPAVAGASVDRVGTEGVGWSWG
                     FQQTAADGSTALSLVAPERSYRKGSTVQERFNDPVFSPVLPVGRPTSLARKGDEIGFGL
                     PLFGDGAGNGGSSAVSSARTTLLRDGVRVGETAFPGNGLFKVPGGAADYRVETEAARPA
                     GVSEFATRVAAAWTFRSDTAPGGELRPLPLTVVRFAPELTAGSAPRGAELRVPLVVRQQ
                     ATDGIGRLDVEVSFDDGRTWAAVPVSDGVARVANPDAAGFASLRVRASDRVGNTAEHTV
                     IRAYKIV"
     gene            complement(4157..4618)
                     /locus_tag="BN6_RS09170"
                     /old_locus_tag="BN6_18670"
     CDS             complement(4157..4618)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN6_RS09170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BN6_18670"
                     /product="hypothetical protein"
                     /protein_id="WP_148302785.1"
                     /transl_table=11
                     /translation="MALLLLVPACGADVGRACTMIGAPTGVNVEVALATAETGTIEVCW
                     DGRCVQPRLDLLPASRAGDTTCTGTAPEDTCGASAVPTGGKTGFAAIEDLPAKQVTVRL
                     RLADAAGAPVVDRELTPTARTVYPNGPDCGGAGPQLSLAVGADGSVAQR"
     gene            complement(4660..5004)
                     /locus_tag="BN6_RS09175"
                     /old_locus_tag="BN6_18680"
     CDS             complement(4660..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099301.1"
                     /locus_tag="BN6_RS09175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18680"
                     /product="hypothetical protein"
                     /protein_id="WP_015099301.1"
                     /transl_table=11
                     /translation="MTQPQRDRLAAAQAELLHSLMADGPPPAGFDPERLRVEARSLLNK
                     RRGIVAMLRPEVAAELGERFRPLFDEYARAHPRRTGSRFREDAAAFAAWATERGELAPP
                     PKRRWWRRAT"
     gene            complement(5001..5792)
                     /locus_tag="BN6_RS09180"
                     /old_locus_tag="BN6_18690"
     CDS             complement(5001..5792)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099302.1"
                     /locus_tag="BN6_RS09180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18690"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_015099302.1"
                     /sec_met_domain="DUF692 (E-value: 2.4e-84, bitscore: 281.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLGVGIGWRSEIDLTVERLDVDFVEVVAENLHVGHLPESVLLLR
                     ERGLPVLPHAVSLSLGGAEPVDAGRVAHLGALAEALDAPFVSDHVCFVRAGGLDSGHLM
                     PLPRTRDALDVLVANVKAAQADLPVPLALENVAALLEWPDGELTEGQFLADLVERTDCL
                     LLIDVANLYANARNLGTDVGRFLDEIPLERLAYVHVAGGEEHHGVYHDTHAHAVLPEVL
                     DVLAQLCERIDPPGVLLERDDDYPADAELAGELAAIRAVLG"
     misc_feature    6186..6199
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.39"
                     /tool="antismash"
     gene            complement(6645..7457)
                     /locus_tag="BN6_RS09190"
                     /old_locus_tag="BN6_18710"
     CDS             complement(6645..7457)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="BN6_RS09190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18710"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_015099304.1"
                     /transl_table=11
                     /translation="MATSWTPTSAGSAAELWPEEQGFLAGGPGRAAEVAVVSLVEAGAV
                     RMSRHGRVSAVHAQHRSWTPLQARVLQSLPQALGDLVASAARSGEVESLRRHLVDRGFV
                     RSTRRRSAMRSVRRVLVFGGIVTVALGIGDVVPFKGVVAALLCTIFACIALTRLALPLT
                     VSGQAAVKRLDGLATTNNRVSLVAYYGLLGKVQGHLVWEVLGIAPTAAATLQRRKRSDN
                     ATGSASCGGGCGSCSSNSCGSASGSGGSSGSSCGGGSSCGGGCGGGGD"
     gene            complement(7414..8325)
                     /locus_tag="BN6_RS09195"
                     /old_locus_tag="BN6_18720"
     CDS             complement(7414..8325)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="BN6_RS09195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18720"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_015099305.1"
                     /transl_table=11
                     /translation="MAFLWWYGPALVLAVLLGLWLKRVTGSAPVRPLTFEEIGFLGGGP
                     VRAAEVAVAGLVADNRARLAGGTLTAVPAPEPGTSTALQNVLLARLDEPRDLDGLLVEV
                     ATSGPARRIGQGLVEAGLLVSPRRRLVRAVLCVLPVLALMAVGWVVAPAGWPVAVLPSV
                     TGVVAVLLLLGPPPVRTRRGARAYDEATAGLAPVDPAEVTARYGLVRRAVAAPPESGGR
                     RADTAVFDGETAEPIGYAGASEQALPRRRRPAVVIEPRRWRKRDGLLVAGGWLAGGWLA
                     SEWLEGDDGGDFLDTDFSGFGG"
     gene            complement(8359..9279)
                     /locus_tag="BN6_RS49500"
                     /old_locus_tag="BN6_18730"
     CDS             complement(8359..9279)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="BN6_RS49500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18730"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_015099306.1"
                     /transl_table=11
                     /translation="MERPWGLSGPEFLELYWIALAVAVAWAIAVRVRLRGTRGGAPAGV
                     LGAYEIAFLTGGPRRVVETAVASLIASDALRPARDGAVSVIGKPVVHNPVDQAVIADAT
                     RYKYRTLTLMITSVSDQGAAGVVGDSLVERGYLVPPRVAKRRLGRSVVPLALLFAIGVF
                     RWANGLVIGAPVGWLTLQLVLTGVLIWLFRKPKYVTPTALGNSAVGLARAGETTGGGGA
                     TGRSGEVWPVALRGFNAHPNIPLRAAARQRPRPRAAQAGVAGGFIGAGASCSGGSSSSC
                     GGSSGSSCGGGGGGGGGCGGGGGGG"
     gene            complement(9507..9944)
                     /gene="msrB"
                     /locus_tag="BN6_RS09205"
                     /old_locus_tag="BN6_18740"
     CDS             complement(9507..9944)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099307.1"
                     /locus_tag="BN6_RS09205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18740"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_015099307.1"
                     /transl_table=11
                     /translation="MEPVLGATPKVVHSELEWREILTPKEYAVLRDAGTEPAWTGEYTD
                     TKSEGVYSCRACGTELFRSDTKFDSHCGWPSFFSPLAADTVILREDRALGMVRTEVLCA
                     TCHSHLGHVFEGEGYATPTDQRYCINSISLRLVENPGGPTD"
     gene            complement(9954..10292)
                     /locus_tag="BN6_RS09210"
                     /old_locus_tag="BN6_18750"
     CDS             complement(9954..10292)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099308.1"
                     /locus_tag="BN6_RS09210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BN6_18750"
                     /product="hypothetical protein"
                     /protein_id="WP_015099308.1"
                     /transl_table=11
                     /translation="MASSRAADIPEEDRPSGRTDDRPPNVEDELIGLDPHDPETRAFAE
                     HLDRMQRQTPGFTVEGYLAGVENFADSVNRTGGLRRLTAVAVVLLILLGVAISVWFGLG
                     EILSTFFD"
     misc_feature    complement(10331..10345)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.89"
                     /tool="antismash"
     misc_feature    10771..10786
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.91"
                     /tool="antismash"
ORIGIN
        1 ctggtctgcc ggatcccgtc cagccacacc ttggccacgc cggggtcggc gaccagcgcg
       61 gcgaacaggc cggtcgcgcc gaccttggcc gcgcgggcgg cgaacgcgcc ggcggccggc
      121 aggtcgcggg tgacccgcag gccggccgcg gcgcgcagcc ggtcggcgtc gccggtcacg
      181 atcagcggca cgtcgtcgcg ccgggcgtcg tcgtagccgg cctcggccag gccggtcacg
      241 tcgaacagcc gcgggtcgag cctgccctgc gcgagcggac ccagggcgtc gaccgggacg
      301 acgtgcagcc gcccgtcgcg ccggtaggtc tggaaccggg cgtccgcgcg gccctcaccg
      361 ggggtgatcg agtccagccg atccccgcgg aacaggaccc ggtcgccggt gacgagggtg
      421 acggacgtgg tcctggcggc gtcggcgagt gccccgccgg cctcgaccgg accggccccg
      481 ggaccgggtg cggcggtggc cgcggcggtc ccggagacga gcaggccgac cgccaacgcg
      541 gcggctcccc aagcgccacg cttaccggat ctcaacacag atacctcccg aatctggttg
      601 tgcgcgctgt ggaacgcgcg gtcgggaggc cgctcgacac cggagcggcg gcggttaacc
      661 ggaagtgacg aagcccccca acaacctcga cgctatccac ccctcagagt gagatcagcg
      721 tctcattgag ggatgtttag ccaccggatc gggggttttc gacgtgacgc acggtgcgtg
      781 gctagtacgg aagagtgccc ggcgaaccgg gcactgtccg cgtcagacga tcttgtaggc
      841 ccggatcacg gtgtgctcgg cggtgttccc gacccggtcg gacgccctca cccggagcga
      901 ggcgaacccg gccgcgtcgg ggttggcgac ccgcgcgacg ccatccgaga cggggaccgc
      961 cgcccacgtc cgcccgtcgt cgaaggagac ctcgacgtcg agccgcccga tcccgtcggt
     1021 cgcctgctgc cgcaccacca gcggcacccg cagctccgcg ccgcgcggcg cgctgccggc
     1081 ggtgagctcc ggcgcgaacc ggacgacggt cagcggcaac ggccggagct ccccgcccgg
     1141 cgcggtgtcc gagcggaacg tccacgccgc cgcgacccgg gtggcgaact ccgacacgcc
     1201 cgccggccgg gccgcctcgg tctccacccg gtagtccgct gcgccgccgg gcaccttgaa
     1261 cagcccgttg cccgggaagg cggtctcgcc gacccgcacg ccgtcgcgca gcagcgtggt
     1321 gcgggccgac gacacggccg aactgccgcc gttgcccgcg ccgtcgccga acagcggcag
     1381 cccgaagccg atctcgtcgc ccttgcgcgc caacgacgtc ggccggccca ccggcagcac
     1441 gggcgagaac accgggtcgt tgaaccgctc ctggacggtg gaacccttgc ggtaggagcg
     1501 ttccggggcg accagggaca gcgcggtcga cccgtcggcg gcggtctgct ggaagcccca
     1561 gctccacccg acgccctcgg tgccgacccg gtcgaccgac gcgccggcca cggccggcat
     1621 ccagccccag cccgtggtgc cgtccggcgc cgccgggttg ccgacgtgcc cgtaggtcga
     1681 ccccgccggg cccgcgccgt aactcgtgcg cacctcggcc aggtcctcgg ccggcaccgc
     1741 gcgggcgaac ccggtcggca gccggccgtg cagcgcccac gcgaaccggt aggtcaccgg
     1801 ggtggggccc gccggcgtgc ccgacagttg cgcgccgagc agcgcggtca gctcctcgcc
     1861 cggcaggtcc ggaccgttgt ggccgatcga cacgtccgtc ccgaacccgc ccaggaacgc
     1921 cgtgctcacg ctcgccgacc ggtcaccgcg ccgacgggcc gtggtgaggt cgccgagcac
     1981 ctcccgcgct ttcgggtcgg gagcggtgat ccgcaccggt cgagccgtgc gcgcgtccac
     2041 gacgaccgtc gtgtcggcgg tgacggacag gtcggggcgg gggagcagcg cgaccttccg
     2101 gtcggtggtg acgtccgcgc ccacgaagta gtcgccctta gacacgcgca gcgagacctt
     2161 gccgtccgcg ctgaacaaga acacgaacgt gctgttgtcc atgccgatga cggaggcgta
     2221 gtagttcttg gccgggttgc ccgccgcgtc cacgaaccgg aacgtcacgt cgtagctctc
     2281 cacctcgcgg tgcacgctca ccggcacgcg caacggcgtc gcaccgcccg tcgcgacgat
     2341 cgtgcccgcg tacacgccgt ccaccggcgc acggcgggtg tcggcggtga cggcgatcgt
     2401 cgcgtcaccg cccggtggca ccgtgacctc cgccggtgac acggtgacca cgtcgctcgg
     2461 cttgccgtcc ggcgcggtca ccaccgcaga cggccgcagg gtcacgggcg cggtgccggt
     2521 gttgcggtag acgacgttct tcgtcaccgg ggtgtcgtcg tcgtgcggcc acttctgcaa
     2581 gcccagcgcc acgttcgccg ggtcggcggt gaccgtcgtg gtgatggccg cggccaggtc
     2641 gacccggccc gcgccctggt cgaacgcggt cagcgccggg ttcggccggg cggaggcgac
     2701 cagcgcggcc ttgagccgcg cgccggacca gtccgggtgc tgctgcgcca gcagcgccgc
     2761 cgcgcccgcg acgtgcgggg tggccatcga cgtgccggac agggcgacgt gcgtggcgtc
     2821 cacgggcgtg ccgatcgtgc cgtgctcggc cttcgccgcc gcgatgtcca cgccgggcgc
     2881 ggtcacgtcc ggcttcacgc caccgtcggg acgcgggccc cggctggaga actccgcgat
     2941 gccctccgcc cggtccaccg cgcccacggt cagcgcggcg tcggcgctgc cgggggagga
     3001 gacggtgccg ggccgccccg agttgcccgc cgcgaccacg aacagggcgc gcgcggacag
     3061 cgcctcgacg gcggcctcca gcgggtcggt ctccggggtg tcggggccgc ccaggctcag
     3121 gttgaccacg tccgcgccct gcccgaccgc ccactgcacc ccggccagga tcgccgactc
     3181 cgggcagccc gccgccacgc agaccttgcc gtccagcagt tccgcgtccg gcgcgacacc
     3241 ccggtacacg gcgtgccggc tgccgatggc ggcggcgacg tgcgtgccgt ggccgacccg
     3301 gtcggtgttg tccgggtccg tggtgaagtt cgacctcgcc gcgacccgcc ccgccaggtc
     3361 ggggtggccg tcgtcgatcc cggtgtcgag cacggcgacc ttgacgccct cgccggtgta
     3421 gcccgcgctc cacgcggcgg gcgcgccgat ctgcgccgtg ctgcggtcca ggctcggccg
     3481 gcgcagcccg tccagccaca ccttgcgcac gctcgccctc gacacgagcg tgcgccaagc
     3541 gtcggccgca ccgcctttcg gcacctcgac ggcgaccgcg ccgaccgcgt ccagggtctt
     3601 ggtgcgcggc aggccggggt cgccgacgac gatcagcggg atcgtcgggc ggcggtcgta
     3661 gcccgcctcc agcagggccg tgacgtcgaa caggcgcggg tccagccggc cgcgcgccag
     3721 gtcgtgggcc gcgtcgcgcg gcaccacgtg caggtggccg tcccggcgga acgtgtggaa
     3781 cacggcccgc tcccggcccg gaccgccggt cagcgaaccg acctggtcac cggtcaggac
     3841 gacccggtca ccggtgacca gggtgatcga gcgcgcgccg gccgcgactt cgccggccgg
     3901 ggaccggccg cccgccggcg tcgccgcggt gaccagaccg aacagggcca gtgcggcgca
     3961 caacgcgccg cgcccgcgtg ggggtgtcaa cggacctacc tcccggtggt ggtgccgcgc
     4021 gcggcggatc gcacagccgg agggctcggc cggccgcggt cgcggcgacg gcaccgggag
     4081 gtggccggaa acccaggacc cgcccgacgc tagcccgacc cgtcggcggc aggcacccgc
     4141 tgatcggccg gtgtcgtcag cgctgggcca ccgacccgtc cgcgccgacc gcgagggaca
     4201 gctgcgggcc cgcaccgccg cagtccggac cgttggggta gaccgttctc gcggtcggcg
     4261 tgagctcccg gtccaccacc ggcgcccccg ccgcgtcggc cagccgcaac cgcacggtca
     4321 cctgcttggc cggcaggtcc tcgatggcgg cgaacccggt cttcccgccg gtcggcacgg
     4381 cgctcgcgcc gcacgtgtcc tccggcgcgg tgcccgtgca cgtggtgtcg ccggcccggg
     4441 acgccgggag caggtccagt cgcggctgca cgcaccggcc gtcccagcac acctcgatcg
     4501 tgccggtctc ggcggtggcg agcgcgactt ccacgttgac gcccgtcggc gcgccgatca
     4561 tggtgcacgc ccgcccgacg tccgccccgc atgccgggac gagcagcagc agggccaaga
     4621 gtggtctcca catgcccggg ggacggaacg gcagggcggt caggttgccc gccgccacca
     4681 acgccgtttc ggcggcgggg ccagttcgcc gcgctcggtg gcccacgcgg cgaacgcggc
     4741 ggcgtcctcc cggaaccggc tgccggtgcg gcgcgggtgc gcgcgggcgt actcgtcgaa
     4801 cagcggccgg aagcgctcgc ccagctcggc ggccacctcc gggcgcagca tcgccacgat
     4861 cccgcgccgc ttgttcagca gcgaccgcgc ctcgacccgc agccgctccg ggtcgaaccc
     4921 cgccggcggc ggaccgtcgg ccatcagcga gtgcagcagc tcggcctgcg ccgccgcgag
     4981 ccggtcgcgc tgcggctgcg tcacccgagc accgcccgga tcgcggccag ctcaccggcc
     5041 agttccgcgt cggccgggta gtcgtcgtcg cgctccagca gcacgcccgg cgggtcgatc
     5101 cgctcgcaca gctgcgcgag cacgtcgagg acctccggca ggaccgcgtg cgcgtgcgtg
     5161 tcgtggtaga cgccgtggtg ctcctcgccg cccgcgacgt gcacgtacgc caagcgctcc
     5221 aacgggatct cgtccaggaa ccggcccacg tcggtgccca ggttgcgggc gttggcgtac
     5281 aggttcgcca cgtcgatcag gagcaggcag tcggtgcgct ccaccaggtc ggccaggaac
     5341 tggccctcgg tcagctcgcc gtcgggccac tccagcagcg cggccacgtt ctccagcgcc
     5401 agcggcaccg gcaggtcagc ctgcgcggcc ttgacgttgg ccaccaggac gtccagcgcg
     5461 tcacgggtgc gcggcagcgg catcaggtgg cccgagtcga ggccgcccgc gcgcacgaag
     5521 cacacgtggt cgctgacgaa cggcgcgtcg agcgcttcgg cgagcgcccc gaggtgcgcg
     5581 acccgcccgg cgtccaccgg ctcggcgccg cccagcgaca gcgacacggc gtgcggcagc
     5641 accggcagac cgcgttcgcg cagcagcagc acggactcgg gcaggtgccc gacatgcagg
     5701 ttctccgcca cgacctcgac gaagtccacg tcgagccgct ccaccgtgag gtcgatctcc
     5761 gaccgccacc cgatcccgac accgagtttc atgcccgcca ccccatcacg cccgaccggg
     5821 cgaccaccac ggccgccgtt gcaccatggt cgccgcgcgc ggcgacagcc cgaggttctc
     5881 ccacaaccga cgcgaccgca tccggccgtg caacccgcag cgcgcggtca acgccaccac
     5941 ctcggcccgc gcggtgagcg gatcgaggtc gcgcagtcgt ttgagccccc gcgtggtcga
     6001 cggccccacg tagcgcagca gaccggcgag cagtcccaat acgccgtacc ccaggaggtg
     6061 tgacgtgagc gcggacagct cgtagtgcca ctccgtgctg atcacgccga tccaggagac
     6121 gaccgagccg gtcacagccc aggtggcgat gacctgccgg ttgcgcggga tggcgaggcg
     6181 gtgggcgatc acgcgttcgc cgagcgcgcg ggtctcggcg gaggccgcgg tgctcgccag
     6241 cacgacgtcc aacggcgcgg gcagtaaacg caacacgtgt tgcgtcagcg gtcggaacga
     6301 caacgggtga tccgctgtcc cgacgcctga aagcgactcg tgttgcgtta gcgtgcctgc
     6361 tggcgacagc tccgaccacg tcgacatcgt cgcggttgtc ggctcggcca ccggaagcga
     6421 ctcgtgttgc gtcagcggag tgggggaacg cgactcgtgt ggcgttaggg ggctcgccaa
     6481 acgcgacacg tgttgcgtta cggggtgcac cacgccgtcc ggggtgatcc gcgccgcgcc
     6541 ggcctcgacc agcacgacca ccgccacctc ggcggcccgc tgtgggccgc ccgcgatgaa
     6601 gccgtactcc tcgggcagca accaccccgg cgcggtgtcg gtcatcagtc gccgccgccc
     6661 ccgcagccgc cgccgcacga cgacccgccg ccgcacgacg atccgctgct gccgcccgac
     6721 ccggacgcgc tgccgcagct gttgctggaa caactgccac acccgccgcc gcacgacgcg
     6781 ctgccggtcg cgttgtcgct gcgcttgcgc cgctgcaacg tcgcggcggc cgtcggggcg
     6841 atgccgagga cctcccacac caggtgtccc tgcaccttgc ccagcagccc gtagtacgcg
     6901 accagcgaga cgcggttgtt cgtcgtggcc aagccgtcga gccgcttcac cgccgcctgc
     6961 ccggagacgg tcaacggcag tgcgagcctg gtcagcgcga tgcacgcgaa gatcgtgcag
     7021 agcagcgccg ccaccacgcc cttgaacggc accacgtcgc cgatgccgag cgccacggtc
     7081 acgatcccgc cgaacaccag cacccgccgc accgaccgca tcgccgagcg ccgccgcgtg
     7141 gaccgcacga aaccgcggtc gaccaggtgc cgccgcaacg actccacctc gccgctgcgc
     7201 gcggccgacg cgacgaggtc acccaacgcc tgcggcaggg actgcaacac gcgcgcctgc
     7261 aagggcgtcc acgagcggtg ttgcgcgtgc accgcgctca cccggccgtg gcgcgacatc
     7321 cgcaccgcac ccgcctcgac cagcgacacc accgcgacct cggccgcccg gccgggtccg
     7381 cccgcgagga agccctgctc ctcgggccac agctcagccg ccgaacccgc tgaagtcggt
     7441 gtccaggaag tcgccaccgt cgtccccctc caaccactcc gaggccagcc acccgccggc
     7501 gagccagccg cccgcgacga gcagtccgtc gcgcttgcgc caccgccgcg gctcgatgac
     7561 gaccgccggc cgcctgcgtc ggggaagcgc ctgctcggac gctccggcat acccgatcgg
     7621 ttccgccgtc tcgccgtcga acaccgcggt gtccgcgcgc cgtccgcccg actccggcgg
     7681 ggctgccacg gcccggcgca ccaggccgta gcgggcggtc acctcggccg ggtcgaccgg
     7741 ggcgagcccg gcggtggcct cgtcgtacgc ccgcgcccca cgccgggtgc gcaccggcgg
     7801 cggtcccaac agcagcagca ccgccaccac cccggtcacc gacggcagca cggcgaccgg
     7861 ccacccggcc ggtgcgacca cccacccgac cgccatcagg gccagcaccg gcagcacgca
     7921 gagcaccgcc cgcaccagcc gtcgtcgcgg cgacaccagc agtcccgcct cgaccagacc
     7981 ttgaccgatc cgccgggcgg gcccgctggt cgcgacctcg accagcagcc cgtccaggtc
     8041 gcgcggctcg tccaaccggg ccagcagcac gttctggagg gcggtcgacg tgccgggttc
     8101 cggcgcgggc acggccgtca gcgtgccgcc ggcgagccgg gcgcggttgt ccgcgaccag
     8161 gcccgcgacc gcgacctccg ccgcccgcac cgggccgccg ccgaggaagc cgatctcctc
     8221 gaaggtcagc ggccggaccg gcgcggagcc ggtgacccgc ttcagccaca agccgagcag
     8281 gaccgcgagc accagcgccg gtccgtacca ccacaggaac gccatgccgt cagcctagga
     8341 gcgcgggctg tgcgcgggct aacctccgcc gcccccaccg ccgcacccgc cgccaccgcc
     8401 gccgccaccg ccgcacgacg acccgctgct gccgccgcag gacgacgagg aaccgccgct
     8461 gcacgacgcc ccggcaccga tgaacccgcc ggcgacaccg gcctgtgccg cgcgcgggcg
     8521 cgggcgttgc cgagccgcgg cccgcagcgg gatgttcggg tgcgcgttga acccgcggag
     8581 agcgaccggc cagacctccc cggaccggcc ggtcgcccct ccacccccgg tggtctcgcc
     8641 tgcgcgagcc aggcccaccg cgctgttgcc caacgccgtg ggagtcacgt acttcggctt
     8701 gcggaacaac cagatcagca cgccggtgag caccagttgc agcgtcagcc agccgaccgg
     8761 cgcgccgatc acgagcccgt tcgcccagcg gaacacgccg atcgcgaaca gcagcgccag
     8821 cggcaccacg ctgcgcccca gccgccgctt cgcgacccgc ggcggcacca ggtaaccccg
     8881 ctccaccagg ctgtcgccga ccacgcccgc cgcgccctgg tcggacacgg aggtgatcat
     8941 cagcgtgagc gtgcggtact tgtagcgggt cgcgtcggcg atcacggcct ggtcgaccgg
     9001 gttgtgcacg acgggcttgc cgatgacgct caccgcgccg tcgcgggccg ggcgcagcgc
     9061 gtcggaggcg atcagcgagg cgaccgcggt ctccacgacc cggcgcggac cgccggtcag
     9121 gaacgcgatc tcgtaggccc cgagcacgcc cgcgggggcg cctccccgcg tcccccgcag
     9181 gcgcactcgg acagcgatcg cccaggccac tgcgacggcc agcgcgatcc agtacagctc
     9241 aaggaattcc gggcccgaca agccccacgg tcgctccacg gtccaccccc tgtgggcctc
     9301 ggcggacacg cggtccgcgc tagggggcaa tcgtgtgcgt caggccacac cccagcaaga
     9361 gggctttagc aaaccttagg gttgctgttg ccctcgatca ggacgacgat gcgccgtcca
     9421 cgccgccgga accctcgccg tcggtgccct tgaagatgcg catgattcct cctggtgtcg
     9481 atgtctcatc attcggagta gcgccttcag tctgtcggac cgcccgggtt ctcgaccaga
     9541 cgcaaactga tgctgttgat gcagtagcgc tggtccgtcg gggtcgcgta gccctcaccc
     9601 tcgaaaacgt gcccgaggtg gctgtggcat gtggcgcaga gcacctcggt gcgcaccatg
     9661 cccagcgccc ggtcctcccg caggatcacc gtgtcggcgg cgagcgggga gaagaacgac
     9721 ggccagccgc agtgcgagtc gaacttggtg tcgctgcgga acagctcggt accgcaggcg
     9781 cggcaggagt agacgccctc gctcttggtg tcggtgtact cgccggtcca ggcgggctcg
     9841 gtgcccgcgt cgcgcaggac cgcgtactcc ttgggcgtga ggatctcccg ccactccagc
     9901 tcggagtgga ccaccttcgg ggtggcgccg aggacaggtt ccatacctcc acgttagtcg
     9961 aagaacgtgg acaggatctc gccgagcccg aaccacacgc tgatcgccac acccagcagg
    10021 atcagcagca ccacggccac cgcggtgagc cggcgcagcc cgccggtccg gttcaccgag
    10081 tcggcgaagt tctccacgcc cgccaggtag ccctcgacgg tgaagccggg ggtctggcgc
    10141 tgcatccggt ccaggtgttc ggcgaacgcc ctggtctccg ggtcgtgcgg gtcgaggccg
    10201 atgagctcgt cctccacgtt cggcgggcgg tcgtcggtcc ggccggacgg ccggtcctcc
    10261 tccgggatgt cggcggcacg cgaactggcc atgcgccaag ggtagtggca ggttcaaccc
    10321 ggacaccgga cggaacggac tttccgcccg agcccgcgac ggtccgtgcc cgtccgcctg
    10381 cgcctgttcg tgcccggtca gcccgcgagc acggccgccg ggcggaccgc gccgaccggc
    10441 cggccgtggc ccagcaccgg gaaggtcgcc aggtccgccg gcgcgtcgac gcccagccgg
    10501 cgcagcgcgg cgaccagcac cacggcccgc gcccggctcg acccgtcggc gatcttgcac
    10561 gccacggcct cgccggtggg cagcccgatc gcgtagacgc cctcggcacc gtccttggcg
    10621 accgcgccgg ggatcgcccg catcagcgcc gtgacgtcgc gcccggtgcc gccgacccac
    10681 tccgggtggc tgctcatggc ccgcgcgatc cggcgttcgg gaccgtcggt cgcggcggcc
    10741 agccgggcga acgcgcgggc cagcccgacc aggctgatgc cgaacagggg ag
//
