LOCUS       NC_019693              20927 bp    DNA     circular CON 07-JUN-2024
DEFINITION  Oscillatoria acuminata PCC 6304, complete sequence.
ACCESSION   NC_019693
VERSION     NC_019693.1
KEYWORDS    GSC:MIGS:2.1; RefSeq.
SOURCE      Oscillatoria acuminata PCC 6304
  ORGANISM  Oscillatoria acuminata PCC 6304
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Oscillatoriales; Oscillatoriaceae; Oscillatoria.
REFERENCE   1  (bases 1 to 7689443)
  AUTHORS   Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N.,
            Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R.,
            Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C.,
            Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K.,
            Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G.,
            Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and
            Kerfeld,C.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Finished chromosome of genome of Oscillatoria acuminata PCC 6304
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7689443)
  AUTHORS   Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N.,
            Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R.,
            Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C.,
            Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K.,
            Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G.,
            Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and
            Kerfeld,C.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUN-2012) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Oscillatoria acuminata PCC 6304
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: USA
            environment                       :: Missing
            num_replicons                     :: 3
            ref_biomaterial                   :: PCC 6304
            biotic_relationship               :: Free living
            trophic_level                     :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            sequencing_meth                   :: WGS
            assembly :: Newbler v. 2.3 (pre-release), VELVET v. 1.0.13, Parallel
            phrap v. 4.24 SPS
            finishing_strategy                :: Finished
            GOLD Stamp ID                     :: Gi04162
            Funding Program                   :: DOE-GEBA 2009
            Gene Calling Method               :: GenePrimp, Prodigal 1.4
            Isolation Site                    :: soil
            Isolation Comments                :: isolated by MB Allen
            Temperature Range                 :: Mesophile
            Gram Staining                     :: Gram-
            Diseases                          :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Finished
            Current Finishing Status          :: Finished
            Assembly Method :: Newbler v. 2.3 (pre-release), VELVET v. 1.0.13,
            Parallel phrap v. 4.24 SPS
            Genome Coverage                   :: 30x
            Sequencing Technology             :: 454/Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000317105.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 01:39:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,147
            CDSs (total)                      :: 6,067
            Genes (coding)                    :: 5,990
            CDSs (with protein)               :: 5,990
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 12 of 77
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:52:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6191154
            Orig. end                         :: 6212081
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003607.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4086915
            Source DNA and Organism available from Cheryl A. Kerfeld
            (CKerfeld@lbl.gov)
            Contacts: Cheryl A. Kerfeld (CKerfeld@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20927
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="7"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20927
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20927
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10927](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10927)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    473..486
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            478..1326
                     /locus_tag="OSCIL6304_RS24020"
                     /old_locus_tag="Oscil6304_4864"
     CDS             478..1326
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OSCIL6304_RS24020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Oscil6304_4864"
                     /product="hypothetical protein"
                     /protein_id="WP_015150987.1"
                     /transl_table=11
                     /translation="MLKQIQKMLIPSELNKSYILLFLILFLSIFSSYSCINGFWIDREI
                     CGVWLEELSINLIAEVTGILLVLFSVNKAVREGREKEKSKFKEIAFRQLKFTIRKQIYL
                     FFEILKASTPQKPEKTYKQLEDLFDDTYFREVQNLDLLTMAPVLTPAGEPMDWLDYLVY
                     ESLNLRTALGRVVDRYAFYLESEVVDLMEEMTDASFIRFLTSLWEAKRLNGLTSKGDLL
                     FACDDMFREYADSLVKLVKFYNENVTRDRQIIIDEQQWQEWWNHKGRPQLGDSRIKIEI
                     Q"
     misc_feature    1470..1483
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.99"
                     /tool="antismash"
     gene            1845..2306
                     /locus_tag="OSCIL6304_RS24025"
                     /old_locus_tag="Oscil6304_4865"
     CDS             1845..2306
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /locus_tag="OSCIL6304_RS24025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4865"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_015150988.1"
                     /transl_table=11
                     /translation="MAGVLKLDIRESSEELKSLLAKQTTARGRERVQALYLLKIGQIKT
                     LKELSILLGRDTATIYRWFQKYKNHGLQGMLSIKKGQGRKPAIPPNTIERLKQKLQGPE
                     RFNSYGEVKRWLKEECGVDASYKVVHETVRYKLKFKLKPQRKKRVKASN"
     gene            complement(2401..3129)
                     /locus_tag="OSCIL6304_RS24030"
                     /old_locus_tag="Oscil6304_4866"
     CDS             complement(2401..3129)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OSCIL6304_RS24030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Oscil6304_4866"
                     /product="hypothetical protein"
                     /protein_id="WP_015150989.1"
                     /transl_table=11
                     /translation="MSKLVAEIIRQYHIDAKLRQTMYELYELYYESTSFEIFDDDLNHK
                     NYVIALWDLEKKLQGFSTVAIWDFEFEKQAKRAIFSGDTIVHRDYWGEQTLPLAWCRLA
                     GQIKAQAPTEPLYWFLIVKGYRTYRYLPLFAHQFYPTYRYPTPPEMQRLMDYLAEEKFG
                     SDYDPKTGLIRFPISRGHLRGSWADIKEGYEDKPDVRYFLERNPDYGKGDELVCLMELR
                     EDNMRSHARRGFMAGMNSEG"
     gene            3705..6029
                     /locus_tag="OSCIL6304_RS31475"
                     /old_locus_tag="Oscil6304_4867"
     CDS             3705..6029
                     /GO_function="GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1173: WD-40
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /locus_tag="OSCIL6304_RS31475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4867"
                     /product="caspase family protein"
                     /protein_id="WP_015150990.1"
                     /transl_table=11
                     /translation="MKRVALLIGISQHEAGEDEDPGLPPLPTAKEDVSAIAQVLQHPEQ
                     GGFAPGDVQQLIDPDLQSIRCGIEVLLRDRQPEDLVLLYFSGHAIADSQGSLYFATRNT
                     NREHLTNTAIAAEFLQALLYTSVAQQQIIILDCYFSGGTGLVDVKQQLGGYGKAILTAS
                     TTFQSLNRWGDRIAHPNPAINCLDYSAKQHHLGLSTYTAYFIEGIETGAGDLNRDGTIS
                     LDELHHYASQKVQIIAPALQPAIYALPEIGAVALAKAPQDNPHLTYQLAVENFLGRSGG
                     TLSLLSLRSLEIRRESLGISATDALIIQENALKPYRHYQTKLQLYKTVVRSVMQLQDLP
                     LSPEIEADLQHLRRDILGLGKLDVRFIEEPLYPSDLEKSFPTLMVAWASITFLLVFGSY
                     SIYKYLTDPTPSPAISTWLEAIPIPSFSLATPDPSPEAESEPESPTQPEAKPRRPQNKT
                     LRPLPPRPQAVPFQIPTEDPLVLTGHVGPVQGLAVDTQGTLLISGSWDNTLKIWDLNTG
                     EVQETLRPDRPSVIRDVALDPYTQRFASARDDGTIEIWQLDRQGSGLMVELEQSIAGHS
                     GPVYAVAISPDGLTLVSGSQDNTIKIWAIETGDLLHTLTDHRGPVRAIAISPDGQTLIS
                     GAADATIKIWDLETGELQNTLTDHTRLVRGLAIAPDGKTLASASWDRTLKIWSLTTGEL
                     QNTLIGHTDLVVSVAISPDGSTLVSGSDDDTIKMWDLSTGEELATLTNHLSDVFSLVFS
                     LDGKTLVSASWDQTIRVWRSP"
     gene            complement(6267..6800)
                     /locus_tag="OSCIL6304_RS24040"
                     /old_locus_tag="Oscil6304_4868"
     CDS             complement(6267..6800)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /locus_tag="OSCIL6304_RS24040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4868"
                     /product="YcxB family protein"
                     /protein_id="WP_015150991.1"
                     /transl_table=11
                     /translation="MAISLEFTLKRQDFEETLMIYNQSKSYLRYLGIFTTYVIMILFGF
                     LVFLKIYSLINNDLEFSKRVDKSLFVSAGFVLFYNLNFWHKAMTWFMWKVAPFLKEPVF
                     VQIEDSGFSYKNAVMKSEIEWSSVQQVLESKNVFFILHRLKASYTLPKRSFTPEDMNEF
                     QQILAANNIQIERK"
     gene            7145..7864
                     /locus_tag="OSCIL6304_RS24045"
                     /old_locus_tag="Oscil6304_4869"
     CDS             7145..7864
                     /EC_number="2.6.99.2"
                     /GO_function="GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626251.1"
                     /locus_tag="OSCIL6304_RS24045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4869"
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="WP_015150992.1"
                     /transl_table=11
                     /translation="MATLGVNIDHIATIRQARGTVEPDPVAAATLVELAGADGITVHLR
                     EDRRHIQDRDVRLLRETVRTHLNLEMAATDEMVKIALDIKPDYVTLVPERREELTTEGG
                     LDVAGQRDRMEGVVDTLQSAGIPVSLFIDADRAQIEASVEVKAQFIELHTGQYAEAKTE
                     ADRDRELAILAEGCNQAISSGLRVNAGHGLTYWNVYPIAKLLGMEELNIGHTIISRAVL
                     VGLERAVREMKQAMQGH"
     misc_feature    complement(8113..8127)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.11"
                     /tool="antismash"
     misc_feature    complement(8116..8134)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.03"
                     /tool="antismash"
     gene            8238..8561
                     /locus_tag="OSCIL6304_RS24055"
                     /old_locus_tag="Oscil6304_4870"
     CDS             8238..8561
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354641.1"
                     /locus_tag="OSCIL6304_RS24055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4870"
                     /product="MgPME-cyclase complex family protein"
                     /protein_id="WP_015150993.1"
                     /transl_table=11
                     /translation="MQTYHYVLASQKFLLEEEPFEEVLKERTRHYHETEKELDFWQVKQ
                     PAFLEAPEMAEVKAKCPQPSVAVISTNPQFITWLKLRLEYVLTGQFQAPSDTIPDPLAS
                     QEG"
     gene            8614..9288
                     /locus_tag="OSCIL6304_RS24060"
                     /old_locus_tag="Oscil6304_4871"
     CDS             8614..9288
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OSCIL6304_RS24060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Oscil6304_4871"
                     /product="hypothetical protein"
                     /protein_id="WP_015150994.1"
                     /transl_table=11
                     /translation="MRLSLRTCLKRSHSWLSPAFLALSGLTLFAPHGHAQPTYPACQPP
                     LPDEYLVLVPTATVAAQEEIRQLLPGAIETTVCNYVNETVTRLGGFGRANQAQEWAQYV
                     NTQAGIVAYVVRPAGVAATPSPLPVVPQPAGVYNPQPLENGYAVLVDFFSNPQVAGELW
                     QILRTDIGLVSYGQRPYLLAGWSPQQNQANQILQTLSDRGFFATVVDSRNVILLDDAVR
                     LD"
     gene            complement(9530..9985)
                     /locus_tag="OSCIL6304_RS24065"
                     /old_locus_tag="Oscil6304_4872"
     CDS             complement(9530..9985)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619999.1"
                     /locus_tag="OSCIL6304_RS24065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4872"
                     /product="divergent PAP2 family protein"
                     /protein_id="WP_015150995.1"
                     /transl_table=11
                     /translation="MQEIGNIIDNRVLLVATLACLIAQSAKLAIELARNRKFNFRVLVE
                     TGGMPSAHSALVTALATGIGQTAGWNSTEFALATIFAVIVMYDAAGVRQAAGKQARILN
                     QIVDEFFSEDHDLNEARLKELLGHTPFQVIVGALLGVCIAYIAGQAY"
     gene            complement(10001..10927)
                     /gene="crtE"
                     /locus_tag="OSCIL6304_RS24070"
                     /old_locus_tag="Oscil6304_4873"
     CDS             complement(10001..10927)
                     /EC_number="2.5.1.1"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0033386 - geranylgeranyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="crtE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634137.1"
                     /locus_tag="OSCIL6304_RS24070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4873"
                     /product="geranylgeranyl diphosphate synthase CrtE"
                     /protein_id="WP_015150996.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 6.8e-64, bitscore:
                     214.2, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLTNDTDSVRKQSEFDLSTYLGERQALVEAALDQSLPIKPPSTI
                     YEAMRYSLLAGGKRLRPILCLASCELVGGTVEMAMPTACALEMVHTMSLIHDDLPAMDN
                     DDYRRGKLTNHKVYGDDIAILAGDGLLVYAFEVVATQTQNVPAPRVLKAIAHLAHAFGA
                     DGVVGGQVVDIQCEGKLDTTVETLHFIHTHKTGALLEACVVCGAILGGAADEDLQRLSR
                     YAQNIGLAFQIIDDILDITKTQEELGKTAGKDLQAQKATYPSIWGLEESTRQAQELIEQ
                     AKAELASFGNKAIPLLTLADFITNRSN"
     misc_feature    complement(11134..11148)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.02"
                     /tool="antismash"
     misc_feature    11135..11148
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.88"
                     /tool="antismash"
     misc_feature    complement(11136..11149)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.16"
                     /tool="antismash"
     gene            complement(11222..12100)
                     /gene="folD"
                     /locus_tag="OSCIL6304_RS24075"
                     /old_locus_tag="Oscil6304_4874"
     CDS             complement(11222..12100)
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /gene="folD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715636.1"
                     /locus_tag="OSCIL6304_RS24075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4874"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD"
                     /protein_id="WP_015150997.1"
                     /transl_table=11
                     /translation="MSKTAVIIDGKAVAEKIQGQLRSQVEELAQKASRPPGLAVLMVGD
                     NPASAAYVRNKERSCGKVGIASYGRHFPEQTSQEELMQAIADLNEDDRVDGILVQLPLP
                     KHLDAVAAINHIHPDKDVDGLHPVNLGRLMRGEPGLCSCTPAGVMALLREYGIDPRGKQ
                     ATIVGRSILVGKPIALMLLAADATVTMAHSRSPDLGAITRNADILVVAVGRPELITADM
                     VKPGAIVIDVGINRVTDYEGKSRLTGDVNYDSAKDVAGFITPVPGGVGPMTVTLLLQNT
                     VESYIRRLGLE"
     gene            complement(12302..13558)
                     /gene="dcm"
                     /locus_tag="OSCIL6304_RS24080"
                     /old_locus_tag="Oscil6304_4875"
     CDS             complement(12302..13558)
                     /EC_number="2.1.1.37"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="dcm"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006624677.1"
                     /locus_tag="OSCIL6304_RS24080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4875"
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_015150998.1"
                     /transl_table=11
                     /translation="MTTIRFVDLFAGIGGIRLGFEKAAEKLALKTHCVLSCEIKADSGW
                     VYQQNFGESPQGDIRILEKLPPHDVLLAGFPCQSFSAAGKKAGFGDTRGTLFFEIIRLM
                     DTSQSQAFLFENVRGLLTNDGGNTIKMIQHQLEARGYSFDIFVLNSANFGLPQNRLRVY
                     LLGLFNAVPKYRLVSDSGPKDSHSYHPQQFSLVNPAAKSVTVADILEDNPPSDYDCSRE
                     FVRALEKRLDGDLNRLHGMRLIDYRGGNSIHSWELGLRGECSADEIELMNRFILKRRNS
                     EFGKEQDGKLLSQEQIATFFKSPELDEILQSLVGKNYLKIVQGKYKPVSGNFSFEVYKF
                     LDPEKIAVTLVASDANRLGVYHQGRVRRITPREAARLQGFPDEFILHPKGDRAYYQLGN
                     SVSINVVEAVAREVILNLK"
     gene            complement(13636..16614)
                     /gene="pruA"
                     /locus_tag="OSCIL6304_RS24085"
                     /old_locus_tag="Oscil6304_4876"
     CDS             complement(13636..16614)
                     /EC_number="1.2.1.88"
                     /GO_function="GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA]"
                     /GO_process="GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pruA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008051273.1"
                     /locus_tag="OSCIL6304_RS24085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Oscil6304_4876"
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="WP_015150999.1"
                     /sec_met_domain="Aldedh (E-value: 2.5e-155, bitscore:
                     516.0, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVQASKNTTYEAKTQEIAKQLLAATQEKRSFFAQLQDQMRWDDK
                     ILDWAMSNPGLRVQLFRFIDCLPALRSKPEIARHLQEYLTVEEVELPDALKKLIGFSGG
                     DSVPGQIAATTVSTAIETLAQKYISGENIKQALKTIERLRKDKMAFTMDLLGEAVITEA
                     EAQSYLERYLDLMTQLTDVAKKWSKVPQIDEADGETIPQVQVSVKLTAFYSQFDPIDAE
                     GSEARVIDRIRTLLRHAQAVGAAVHFDMEQYHYKDLTLSILKRLLMEEEFRSRTDIGVT
                     LQAYLRDSKQDLQGLIDWAKERGNPVTVRLVKGAYWDQETIKAIQKHWPQPVYNDKAAT
                     DVNFEQMTELLLENHEYLYGAIGSHNVRSQAHAIAIAQTLNIPRRRFEMQVLYGMGDKL
                     AKALVQQGYRVRVYCPYGDLLPGMAYLIRRLLENTANSSFVRQSQEDRPIESLIGPPQP
                     DGDEPIAIHNKALPNAAPDLDYAKIKEREEAAAALASVRQQLGKTYLPLINGDRVNAPN
                     TVNSVNPSNPTEVIGTIGLMSVEQADQAVAAAKSALTQWRTTPAGDRANLLRKAGELME
                     QRRHELSAWMVLEVGKPLRECDAEISEGIDFCRYYADEMERLNSGVNYDVAGETNRYHY
                     QPRGITVVISPWNFPFAIPVGMTVASLVAGNCTLLKPAEVSSVIAAKFAEILVEAGIPS
                     GVFQYVPCKGSSVGSHLVKHPDVNTIVFTGSQEVGCQIYAEAAILQPGQKHLKKVIAEM
                     GGKNAVIVDESADLDPAVAGVVQSAFGYSGQKCSACSRVIVLEPVYETFVNRLVEATRS
                     LNVGVAEELSTQVGPVIDANARDRIQGYIAKGKAEAELVLEMAVPETGYFIGPTIFSNV
                     KPDAAIAQEEIFGPVLSVIRAKDFDEALEIANGTNFALTGGLYSRTPSHIDRAYAEFEV
                     GNLYINRGITGAIVARQPFGGFKLSGVGSKAGGPDYLLQFLEPRHVSENVQRQGFAPIE
                     GVD"
     gene            16643..16804
                     /locus_tag="OSCIL6304_RS34690"
     CDS             16643..16804
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OSCIL6304_RS34690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_156823956.1"
                     /transl_table=11
                     /translation="MMGQVSDAIAPLTAKLNIIAFFEVLLKNNSGGKMNVSSICTQVTS
                     EDEPDLMQ"
     gene            complement(17115..18020)
                     /locus_tag="OSCIL6304_RS24090"
                     /old_locus_tag="Oscil6304_4877"
     CDS             complement(17115..18020)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OSCIL6304_RS24090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Oscil6304_4877"
                     /product="hypothetical protein"
                     /protein_id="WP_015151000.1"
                     /transl_table=11
                     /translation="MADLDSNATQTIEQLEILMTHSGQIENSLAQTTAQLSTIAEEVEA
                     NWQDLSNQANELLQRIVTEKEQFIAETEGLKNAIQESKSTIEPVPEEIEEAIESTKGAI
                     SEFNDKVESMAPNLESQVDELAESVSSLTEKTQTVRAEIEEAISESEEYLLNQVNAEIK
                     NYQAELEERVEQLQNYITEECLVKITQKTTDFNEHFNSVVEKLTTKMEDLAETTEQSAE
                     ETLSNLGEQIKDEIYGNLIDKAKEIEQLMERLSRVMDTTTSTVMTSKDLMVDGMNATNL
                     GLNTAFGILEEIKELLESVI"
     gene            complement(18071..18985)
                     /locus_tag="OSCIL6304_RS24095"
                     /old_locus_tag="Oscil6304_4878"
     CDS             complement(18071..18985)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OSCIL6304_RS24095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Oscil6304_4878"
                     /product="hypothetical protein"
                     /protein_id="WP_015151001.1"
                     /transl_table=11
                     /translation="MDLNLLQNQLILAPQNLNNLIAETRQLQIAVNDFNSNMTAKETEI
                     ATTIAQIETAIDELQQAISSQPSELSEGIAELEETLNQFQETISNQQAPMIALIKEGQN
                     QVQSLKAALGEQKTALETSKEQIQADLSETLELLQAQRDKLEEALNATSDEVEVLREKI
                     EAGKSTTQEQVESITSELEESQEQVTEYLQEMATQLTTLQNNLQTNLTTVNQNKFTVEV
                     DRFVQETDQKIKGEIQEMVQSSVNHIKEAIGLMNDRLNDAKSESATERQLIEPLIDQVK
                     TLLEPLESLIDVVQSIADAIPGI"
     misc_feature    18962..18976
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.88"
                     /tool="antismash"
     gene            complement(19037..20035)
                     /locus_tag="OSCIL6304_RS24100"
                     /old_locus_tag="Oscil6304_4879"
     CDS             complement(19037..20035)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OSCIL6304_RS24100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Oscil6304_4879"
                     /product="hypothetical protein"
                     /protein_id="WP_015151002.1"
                     /transl_table=11
                     /translation="MTTVNAPEAEIQNNVENLANFVSLLEGSHAQIVAANSELEADSEN
                     LPDIVALLETSIANLGQSLGNLQQQWTDADTRLNSAQESVAQFTTAAEEQLEPSLESLE
                     ATANSFQSEFNADRQTLAENISEVQNDFVELEEASSIWEDSLVELQETTQNTFNEVEES
                     IAQFQETVDTFKSEAESDFEGLIEDIVEQHNPGLTNLMTDCSNTLSEDYSNQVVTDFDT
                     FETEAINLYETFNSDITNLGNSFQSQGIELINSSVEYCQDSLGNEINSAFDEAINQVIE
                     ELIQEIVTTIVTVKVGATMTTTMSPILPQLVIAKNVIQAINTAKDLFNPFS"
ORIGIN
        1 acgcggtttc ggcgactcct aatcgagatt cggcaatcga gtaagttaac ccaggtgcaa
       61 gtggctcaac gactgcataa accccagtct ttcgtctcca agtacgaacg gggggaacgc
      121 cgtttagatg tggtggagtt tctggaagtt gctaaagcac tggaggttaa tccttcagag
      181 ttgctgactc aactggaccg cccaactgta gagcaaggtt ccacaaacat gggtgaattt
      241 tgaaagcagc gatcgggcag tcgcttgagt tcaaatgagt ctcttgttcc atctctactc
      301 aagtcatcag ggatttgaac atcaggaact ttttcagcca agcaattcgg gaatgaggcg
      361 gggtgaagca caaaggcagc cccaattcca ccggatttta tagaacagga ctaactggcg
      421 atcgcgctta atctctgcac aatactcact cattctgtac aagacagagg gaattttatg
      481 ttaaaacaga ttcaaaaaat gctgattcct tctgaattaa acaaatctta cattctttta
      541 tttttaattc tattcttgag catattttct tcctactctt gtattaatgg attttggata
      601 gaccgggaaa tatgtggggt ctggctagag gagctttcca ttaatttaat tgccgaagtt
      661 accggaatat tattggtttt attctcggtc aataaagccg ttagagaagg gcgggaaaaa
      721 gaaaaaagta aattcaaaga aatcgcattt agacaattaa agtttaccat tagaaaacaa
      781 atttatttat tttttgaaat tctcaaggct tcaacccctc aaaaaccgga gaaaacctat
      841 aaacaattag aagatttatt tgatgatact tattttcggg aagttcagaa cttagacctg
      901 ttaacaatgg ctcccgtgtt aacccctgcg ggagaaccga tggactggct agattacctc
      961 gtttatgaaa gcctgaacct ccgcaccgcc ttgggtcgag tggtcgatcg ctatgccttt
     1021 tacctggaat ccgaggtggt ggatttaatg gaagaaatga ccgatgccag ctttattcgc
     1081 ttcctcacct ccctctggga agccaaacgc ttaaatggat taacctccaa gggagattta
     1141 ctgtttgctt gtgatgatat gttccgagag tatgccgatt ccttggtcaa attagttaaa
     1201 ttttacaatg aaaacgtcac ccgcgatcgg caaatcatca tcgatgagca gcaatggcaa
     1261 gaatggtgga accataaagg cagaccgcag ttaggagata gtcgcatcaa aatcgaaatt
     1321 cagtaatttt attgacctca accatcaact cccttatcta caaaacctgg gggtaaagga
     1381 aggtatctgt aggggcgcaa tgcgcaggcc cctagggcct gcgcattgcg cccctacatt
     1441 gacggggtta ctccgttgat ccgtcactac gaacgaacgt tttggagatt tgattttttg
     1501 gagttcccta actgtaggac ttacgcaatc tttaatatta gacgctaaat gtagaggcga
     1561 ttcgctttct cgcctctaca tttagcgtaa attgtcaaaa tctgcgtaag ttctgaactg
     1621 attagggcca gatacccgat ttttggtcct aattagggag gtgtcacccg gcgctggagt
     1681 ggcgatcgca ccgcaggaag tcaactttat cccgttgact cccattcttg tctgagttaa
     1741 ccgttaaaat ttacggcgag tttttcatcg ggttaaaatt gatacaactt cacttttttt
     1801 ctgcaactat aactaataga tgtcgtagcg ttcaggttag tgatatggcc ggggtcctta
     1861 agctcgatat ccgagaaagt agtgaagaac tgaaaagcct tttggctaaa caaacaacag
     1921 caagaggccg ggaaagagtt caggctttat atttattaaa aatcggccaa attaaaaccc
     1981 taaaagagct ttctatttta ttaggacgag atacggccac catttatcga tggtttcaaa
     2041 agtataaaaa ccacggatta caaggaatgc tgtccattaa aaaaggccaa gggcgaaaac
     2101 ccgcaattcc tcccaataca attgaacgat taaaacaaaa acttcaagga cctgaacgat
     2161 ttaacagtta tggagaagtt aagcgatggc ttaaggaaga atgcggggtg gacgcttcct
     2221 ataaagtggt ccatgaaacg gtccgttaca aactaaaatt taaactcaaa ccccagcgca
     2281 aaaaacgggt caaggcgtcg aattaaagcc actctttgac ccaattttta ccctggtgac
     2341 tggccgaggg atggtccgct tatcctttgc cagtcagggg attgctgtcc cgttcatgtg
     2401 ttacccttct gaattcatcc cagccataaa tccgcgtcgg gcgtgcgatc gcatattatc
     2461 ttcgcgcagt tccatcaaac agactaactc atcccccttg ccatagtctg gattgcgctc
     2521 taaaaagtag cgaacatcgg gtttatcttc atatccctct ttaatatccg cccaacttcc
     2581 gcgcagatgt ccccgggaaa tgggaaagcg aatcagtccg gttttggggt cataatccga
     2641 accaaatttt tcctcagcta aatagtccat cagccgttgc atttctggcg gggtcggata
     2701 acgataggtg ggataaaact gatgagcaaa taggggtaaa tatcgataag ttcggtagcc
     2761 tttaacaatc agaaaccaat ataaaggctc tgtaggagct tgagctttaa tttgaccggc
     2821 taatcgacac caagctaaag ggagtgtttg ttcgccccaa tagtcccgat gcacgatggt
     2881 atcccctgaa aagatagctc gtttggcttg tttttcaaac tcaaaatccc agatagccac
     2941 tgtagaaaat ccctgcaatt ttttttctaa atcccataga gcaattacat aatttttatg
     3001 attcaaatca tcatcaaaaa tttcaaaact ggtgctttca taataaagtt catataactc
     3061 atacatggtt tggcgtagtt ttgcatcgat gtgatattgc cggataattt ctgcgactag
     3121 tttgctcata agggattgat ttaatgaagg agaggaaaat aaatcggaaa agatgggcaa
     3181 ccgtagcccg aaaaatttag agtgaccttt gatttggaaa gaggtttaat ctcattcaga
     3241 atgcacccca tcagtttatc aaaaaccctg ggatatgctg gaatagggcg gggtaactgg
     3301 taaaagctgg ggtgcgatcg ccctcgggtt gcccattgct aggggaccca cgggaaaggg
     3361 attcactaaa ccgcactcca cacaggggag tccccgggaa agtattggac aaaatgggta
     3421 ctcccctcgc tctcatttag ggctacaatt tgagaacagc actgaggcca ataggtttgg
     3481 ccagagtaga cattgggaat ccggatctgt tcttcaaggc gggaaccgca agaaactgga
     3541 tgcgagccgt tggaagggaa gggtttaagc gggcaaccca taccctgaga gaacttgata
     3601 gaatagttaa gagttacaga tattgaaaaa gggttcaaat tggatcaaaa tgggcaaatc
     3661 agggtgacac tgaagaagtt gaaaattcag aaattgaaac gtcaatgaaa cgggtcgcac
     3721 tactaatcgg aattagtcaa catgaagcgg gagaggacga agaccctggt ctaccgcccc
     3781 tgcctacggc gaaagaggat gtatccgcga tcgcccaagt attgcagcat cccgaacagg
     3841 gcggatttgc tcctggcgat gtccaacaac tgattgatcc ggacctgcaa agtatccggt
     3901 gcggcattga ggtcctgttg cgcgatcgcc aacccgagga cttggtattg ctctattttt
     3961 ccggtcatgc gatcgccgac tcccaggggt cactgtattt tgccacccgg aacacgaacc
     4021 gcgaacacct cacgaatacc gcgatcgcag cggagttctt gcaggcactc ttgtacacca
     4081 gcgtcgccca gcaacaaatt atcatcctcg actgctattt cagtggcggg actggtttgg
     4141 tcgatgtcaa acagcaattg ggaggctatg gaaaagccat tctcactgcc tctaccacct
     4201 ttcaatccct gaaccgttgg ggcgatcgca ttgctcatcc caatcccgcg attaactgtc
     4261 tcgactattc cgccaaacaa catcacttgg gactctccac ctacaccgcc tacttcatcg
     4321 aaggcatcga aaccggggcc ggggacctca atcgcgatgg tacaatctct ctggacgaat
     4381 tgcaccacta cgccagtcaa aaggtgcaaa tcattgctcc cgccttgcaa ccggcaatct
     4441 acgcactccc tgaaattggt gcagtcgccc tcgctaaagc cccccaagat aaccctcacc
     4501 tgacctacca attagctgtt gaaaattttc tgggacgcag tggcggcact ctttccttac
     4561 tcagcctccg cagtctagaa attcgccgag aaagcctggg tatctctgcc acggatgcct
     4621 tgattattca agaaaatgct ctgaaaccct atcgacacta ccaaactaaa ttacaactct
     4681 ataaaaccgt cgttagatcc gtgatgcagc ttcaggactt gcctctgagt cctgaaattg
     4741 aggcagattt gcaacattta cgccgggata tcctgggtct gggcaaactc gatgtccggt
     4801 tcattgaaga acctctgtat ccttcggatc tggagaaaag ttttcccacc ttgatggtcg
     4861 cctgggcgag tataacgttc ctgttggttt tcggcagtta ttctatatat aagtacctga
     4921 cggacccgac tccttccccg gccatctcca cctggctgga agccatcccg atcccctctt
     4981 tttccctggc aactcccgac ccgagtcccg aagcggaatc cgaaccggaa tccccaactc
     5041 agcctgaagc taaaccgcga cgaccccaga acaaaacact caggccctta ccacctcgtc
     5101 cccaggcggt cccgtttcag ataccgacag aagacccctt ggtactcacg ggtcatgttg
     5161 gcccggttca gggactggca gtcgataccc aaggcactct gttgattagt gggagttggg
     5221 acaatactct gaagatctgg gatttaaaca ctggggaagt ccaagagacc ctcagacccg
     5281 atcgccccag tgtgattcga gatgtggccc ttgaccctta tactcaacgc tttgctagtg
     5341 ccagggatga cgggactatc gagatttggc aactcgatcg ccaagggtcg ggtctgatgg
     5401 tggagttaga acaaagcata gccggtcatt ctgggccggt ttacgcagtc gccatcagtc
     5461 ccgacggact caccctggtg agtgggtctc aagataatac gattaaaatt tgggccatag
     5521 aaaccgggga cctcctccat actctgacgg atcatcgagg acctgtgcgt gcgatcgcca
     5581 tcagtcccga tggacaaacc ctgatcagtg gtgccgccga tgccacgatt aaaatttggg
     5641 acctggaaac gggggagttg caaaataccc tcacggatca tactcgttta gtccgaggac
     5701 tggcgatcgc tcctgatggc aaaaccctcg cctctgccag ttgggaccgc accctgaaaa
     5761 tttggagtct cacaaccggc gagttacaaa atactctcat cggtcatacg gatttagttg
     5821 tttctgttgc cattagtccc gacggttcca ccctcgttag tggtagcgat gatgacacga
     5881 ttaaaatgtg ggatttgtcc actggggagg aactcgcgac cctgactaat caccttagtg
     5941 atgttttttc cctcgtcttt agtctcgatg gcaaaaccct ggtttctgcc agttgggatc
     6001 aaaccatccg agtctggcga tcgccctaaa aacagcggaa ggcccaagtc cgacaccctc
     6061 ccttggactc ccttctgctt cgggagcatc ccaatgggta aaagagacct aaccccccag
     6121 cccccttccc tagaagggaa gggggagaaa gacatttatt ccccccttaa aagccataaa
     6181 aaaattgctt tattctccct ctccttttag gagagggctg gggagaggtc agactgggtt
     6241 ttaggcaaaa tagagatgct actgttctat tttctctcaa tttgaatatt attggcggct
     6301 aaaatctgct ggaattcatt catatcttct ggagtaaaac ttcttttagg cagtgtataa
     6361 gaagccttga gtctatgaag aataaaaaac acattttttg attcaagcac ctgctgtact
     6421 gaactccatt caatttcgct tttcatcacc gcatttttgt aactaaaccc tgaatcttct
     6481 atctgaacaa aaacaggctc ttttaaaaac ggcgcaactt tccacatgaa ccaagtcatt
     6541 gctttgtgcc aaaaattaag attataaaat agcacaaaac cggcagaaac aaacaatgat
     6601 ttatcaactc tttttgaaaa ctctaaatca ttatttatca agctataaat tttcaaaaat
     6661 accaaaaaac caaacaatat cataataaca taagttgtaa aaattcccag ataacgcaaa
     6721 taggacttcg actgattata aatcatcagg gtttcttcaa aatcttgacg ttttaaggta
     6781 aattccagag aaatcgccat aaattcaatt gattaattat ataaaactca gggattaaag
     6841 gtagatgaag gactgttcaa cggatgcctt gattaacgct aaaccttcaa gggtcattct
     6901 caagccagtc ggaagatttt tctagtgtat gtcatctcag cagaaaataa ggtggagggg
     6961 ttactttttc ataatttatt aatctttggt gatgggtcat tggtggttgg tcggtgagta
     7021 ttcaacaatt caaatgacag atgaccaatg accaatgacc aatgaccttg agaaataagg
     7081 cagacacact atagattaaa atcaactaaa cagccgttca ttcaaacatc aggagacatt
     7141 gaccttggct acacttgggg ttaatattga ccacattgcc acgattcgac aagcacgggg
     7201 gaccgtagaa ccggatcccg ttgcggcagc tactttagtc gaactcgcgg gtgccgatgg
     7261 gattacggtg catttgcgtg aagaccgtag acatattcaa gaccgggacg tgcgtttgct
     7321 gagggaaacc gtccggaccc atttaaacct agaaatggcg gcgacggacg aaatggtaaa
     7381 aattgctctg gatattaaac ccgattatgt gaccttggtt ccggaacgac gagaagaact
     7441 gaccacagaa ggtggactgg atgtcgccgg acaacgcgat cgcatggaag gggtggtgga
     7501 taccttgcaa tctgctggaa ttcccgttag tttgtttatt gatgccgacc gcgctcaaat
     7561 tgaagcatcg gttgaggtca aagcgcaatt tatcgaactg catacgggac aatatgcaga
     7621 agctaaaacc gaagccgatc gtgatcgaga attagctatt ttagccgaag gctgtaacca
     7681 ggccatctct tccgggttgc gcgtgaatgc cggacatgga ctcacctatt ggaacgtcta
     7741 tcccatcgcc aaactgttag gaatggaaga actgaatatc ggtcatacca ttatctctcg
     7801 tgctgttcta gtcgggctag agcgtgccgt cagagaaatg aaacaagcga tgcaaggcca
     7861 ttgataaagc ctaccgcaaa aacagcgttt atcttcaggg gttgcttttt ggagctaact
     7921 tttaattaaa attccctgat tttgtccgcg tacaagggtt aaaaatttac cgggatattc
     7981 ccggtaaatt tgctggaaaa cagcgcagtg atctggatta atttgggcgg gatcatctca
     8041 tctagccaat gggggaaaat ttttagcgat cgccaggggt acggatcctg tgtttcaccg
     8101 aactcctggg ataattttga gaaactttgg caagaaaatc gtctaggttt aaagtccggg
     8161 gttaattttt ttttccgcat gagttacttt aatgttgtac gatactcgat cgagacttgt
     8221 acaagccaac aaccaccatg caaacttatc actacgttct ggctagccaa aaattcttat
     8281 tagaggaaga accgtttgaa gaggtgctca aagaacggac gagacattac cacgaaaccg
     8341 aaaaagaact tgatttttgg caggtaaagc aacccgcctt tctggaagcg ccagaaatgg
     8401 ccgaagtgaa agccaaatgt cctcaaccct ccgtggcggt gatttcgacc aatccgcaat
     8461 ttatcacctg gttaaaattg cgtctggaat atgtgttgac cggccaattt caggccccct
     8521 ctgacaccat tcccgatcca ttagcctccc aagagggtta aagaatccgt taaactgcct
     8581 gattaatact tggagagacc cgggatcata actatgcggc tatccctacg aacttgttta
     8641 aagcgatcgc actcctggct gagtccagca ttcctcgccc tgagtgggtt aaccctgttc
     8701 gcccctcatg gtcatgctca accgacctat ccagcttgtc aaccccccct cccagacgag
     8761 tatctggtcc tggtccctac ggccacagtt gctgctcagg aggagattcg gcaattgctt
     8821 cccggggcga tcgagaccac ggtttgtaac tatgtgaatg aaaccgtcac ccgtctgggc
     8881 gggtttggca gggcaaacca ggcccaagaa tgggcgcagt atgtgaatac tcaagccgga
     8941 attgtcgctt atgtagtccg tccggcgggt gtggcagcta cccccagtcc tctcccggtc
     9001 gtcccccaac cggcaggagt ttacaatccc caacccttgg aaaacgggta tgccgtcttg
     9061 gtggattttt tcagtaatcc ccaagttgct ggggagttgt ggcaaatctt gagaaccgat
     9121 attggattag tgtcctacgg acaacggcct tatctgttag ctggatggag ccctcaacaa
     9181 aaccaggcga atcagatttt gcaaaccctg agcgatcgcg gtttttttgc aaccgtcgtg
     9241 gatagccgca acgtgatttt attagacgac gccgttcgat tggactaatc gagtctccag
     9301 tcccttcccc atgtcttggg gaggggacca gaggtggctc gatcagttca gatgcgttag
     9361 gggattgcaa aatataaatc atccaaccgt tcaactgaaa ggaaggtatc tgtcggagta
     9421 aatgcgcagg ccctaagggc ctgcgcattt actccgacat ggacggggct attccgttga
     9481 tccgtcacta agaacgaacg ttttggagat tttatttttt ggagttccct taataagcct
     9541 gacctgcgat ataggcaata caaaccccta aaagcgcccc aacaatcacc tgaaacggag
     9601 tgtgaccgag gagttccttc aggcgggctt cattcaaatc gtggtcttcg ctaaaaaact
     9661 catccacaat ttggttaaga atccgagctt gctttccagc cgcttgtcga actccggcgg
     9721 catcatacat cacgatcacc gcaaaaattg tagccagagc aaactccgta ctattccagc
     9781 ctgcggtttg cccgatgccg gttgctaacg cggtaaccaa agctgaatga gcactgggca
     9841 ttccgcccgt ttccaccaga acacgaaaat taaacttgcg atttctcgcc agttcgatcg
     9901 ctaacttcgc tgattgagcg atcaaacagg ctagggtggc aaccagcagc acgcggttgt
     9961 caatgatatt gccgatctcc tgcatggtgt tttgcgcctt ttaattactg cggttggtga
    10021 taaagtccgc gagggtcaga agcgggatcg ctttattgcc aaaacttgcc agttctgcct
    10081 tagcctgctc aattaactct tgagcctgcc gagtcgattc ttctaacccc caaatactgg
    10141 gataagtcgc cttttgcgcc tgcaagtctt ttcctgcggt tttgcccaac tcttcctgag
    10201 tcttggtaat atcgagaata tcatcaataa tctgaaaggc taacccaata ttttgggcat
    10261 agcgcgagag tcgttgcaaa tcttcatccg cagcgccacc taaaatcgct ccacaaacca
    10321 cacaggcttc cagtaaagcc ccagttttat gggtgtggat aaaatggagc gtttctacgg
    10381 tagtgtctaa cttgccttcg cattggatat cgaccacttg acccccaacc acgccatcag
    10441 cgccaaacgc atgagctaag tgggcgatcg cctttaaaac ccgaggtgcc ggaacattct
    10501 gagtttgggt tgccaccact tcaaaggcat acaccaacaa cccatccccg gccaaaatcg
    10561 cgatatcatc tccataaacc ttatgattcg tcagtttacc ccgacggtaa tcatcattat
    10621 ccatcgccgg aaggtcatca tggattaagg acatggtatg caccatttcc agggcacaag
    10681 cggttggcat cgccatctcc accgtacctc ccaccagttc acaactggct aaacacaaaa
    10741 ttggccgcag acgcttgcca cccgccaaca gggaatagcg catcgcctca taaattgtgg
    10801 acgggggctt gattggcaga gattggtcca gtgcagcttc taccaaagcc tgccgttccc
    10861 ctaaatacgt tgataaatca aattcggatt gtttcctcac ggaatccgta tcattggtta
    10921 aaaccatccc ttccttcctc gtacttgtct gatgagctga cgattccacc cctggaggcg
    10981 gttgacattg atgatgagct tacgctgctt ttggcaccgt ttacaagtga tctaggcgac
    11041 taacctgaac tttggcaacc gggctgattt tggccgcttg ctgctccttg agtctcaacc
    11101 tattgtaagg gtttgaggta tttttactcc tggaattttt tggaaaaaaa tggcgcgaaa
    11161 gccggagttt tgagggcctg attttctctc cctcaaaact ccctcggtga aaatgcccgg
    11221 tttactctaa tcctaagcgc cggatataac tctctacggt attctgtaac agcagggtca
    11281 cggtcatggg tccaacccct ccgggaactg gggtaataaa tccggcaaca tctttggcgg
    11341 agtcataatt gacatctccg gttagcctag atttgccttc ataatctgtg acgcggttga
    11401 tgccaacatc aatgacgatc gccccgggtt tgaccatatc agcggtaatc agttcgggac
    11461 gtcccacggc caccactagg atatcggcat tgcgggtaat tgcgcctaaa tccggcgatc
    11521 gcgaatgagc cattgtgacg gttgcatccg ctgccaacaa cattaaagcg atcggtttac
    11581 ccaccagaat actccgcccg acaatcgtcg cctgtttgcc tctgggatca atgccatact
    11641 ctcggagtag ggccattact ccagcagggg tgcaactaca taatcccggt tctccccgca
    11701 ttaatcgccc caaattcacc gggtgcaatc catccacatc tttatcggga tggatgtgat
    11761 taatggcagc aacggcatcg agatgcttcg gtaaggggag ttgcaccaaa attccatcaa
    11821 cgcgatcgtc ctcgttgagg tcggcgatcg cctgcatcaa ttcttcttga ctcgtttgtt
    11881 caggaaagtg ccttccgtat gaggcgattc ccactttccc acaggaccgt tccttattgc
    11941 gaacataagc agcagaggcg ggattatccc ccaccatcaa aaccgccaac cccggagggc
    12001 gactggcttt ctgtgctaac tcttccacct gcgatcgcag ttggccttga atcttttcag
    12061 cgacggcttt cccgtctatg ataacggctg ttttggacat gataggcttg ggagttgctg
    12121 gaacactccg gctacagcag tgaatattgc tctcttgatt gtctcatagt ctggatgggg
    12181 gagatggggg agaaacggga gaaacgggag atgggggtct aagcagaggc taacttgtca
    12241 taaatcaagg gacaaattca cctcattgct atctggattg tctgtccctc ctttaccctc
    12301 gctattttaa atttaaaatc acctctcgcg ccaccgcttc taccacatta atactcaccg
    12361 aatttcccaa ctgataataa gcccgatcgc ccttcggatg caggataaac tcatcgggaa
    12421 atccctgcaa cctcgccgcc tcccggggag taattctccg cacgcgcccc tgatgataca
    12481 cccccaaccg attggcatca ctcgccacta acgtcaccgc aattttttca gggtctaaaa
    12541 acttataaac ctcaaaggaa aaattacccg aaaccggctt atatttccct tgcacaattt
    12601 tcaggtaatt ctttcccacc aaagactgta aaatctcatc caactccgga gacttaaaaa
    12661 atgtagcaat ttgctcttga ctcagcaact tcccatcttg ctcttttcca aactcgctat
    12721 tgcgccgctt cagaataaac cgattcatca actcaatttc atccgcacta cattccccgc
    12781 gcaaccccaa ctcccaagaa tgaatcgaat tcccccctcg atagtcaatt aatcgcatcc
    12841 catgcaaccg attcaaatct ccatctaatc gcttctccaa agctctaaca aattcccttg
    12901 aacagtcata gtctgacgga ggattatctt ctaaaatgtc cgccacagtt accgatttcg
    12961 cagccggatt aactaaagaa aattgttggg gatgatacga atgagaatct ttcggcccag
    13021 aatcagaaac taaccgatac ttaggaacag cgttaaacaa ccccaagaga taaacccgca
    13081 agcgattttg gggtaaacca aaattagcac tatttagcac aaaaatatca aaactatagc
    13141 ctcgggcttc caactgatgt tgaatcattt taatggtatt ccccccatca ttggttaaca
    13201 aaccccgcac attttcaaat agaaatgctt gagattgaga ggtatccatt aaccgaataa
    13261 tctcaaaaaa tagggttcct ctcgtatccc caaatccggc cttttttccc gcagcggaaa
    13321 aggactgaca gggaaacccc gccaataaaa catcatgagg cggcagcttt tccaaaatcc
    13381 ggatatcacc ttggggagac tcgccgaaat tttgctgata aacccaaccc gaatccgcct
    13441 taatttcgca actcaaaaca cagtgagttt tgagagcaag tttttcggcg gctttctcaa
    13501 agcctaatct tatgccacca atacccgcaa ataaatctac aaagcggata gttgtcattg
    13561 gtcatttgtc cttggttgtt ggtcattggt catttggatt aatgaccaat gaccaatgac
    13621 ccattacaaa ccctcttaat ccacgccttc aatcggcgca aatccttgac gctgcacgtt
    13681 ttcgctgacg tgacggggtt ctaaaaattg cagcaggtaa tccggtcccc ccgcttttga
    13741 acccacccca gacagtttaa acccaccaaa gggttgccgc gcgacgatcg ccccggtaat
    13801 ccctcggtta atatagagat tccccacctc aaactcggcg taggcgcgat caatatgaga
    13861 aggggtccgg gaatataacc cgccagttag ggcaaaattc gtcccattgg caatctctaa
    13921 cgcttcatcg aaatctttag cccgaatcac cgataacacg ggaccaaaaa tctcttcttg
    13981 ggcgatcgca gcatcgggtt tcacgttact aaaaatcgtg ggtccaataa aatagccggt
    14041 ttctggaact gccatttcta ggactaattc cgcctctgct tttcctttgg caatatagcc
    14101 ttgaatgcga tcgcgcgcat tggcatcaat cacgggtccc acttgggtac tcagttcctc
    14161 tgctaccccg acattcagcg atcgcgtggc ttctactaac cgattcacaa aggtttcgta
    14221 cacgggttct aacacaatca cccgcgaaca agccgaacat ttctgtccac tatatccaaa
    14281 cgccgattgc actacccccg ccactgctgg atcgaggtcc gcactttcat ccacaatcac
    14341 ggcatttttg ccacccattt cggcaatcac ctttttcagg tgtttttgtc ccggttgcaa
    14401 aatcgctgct tctgcataaa tttgacagcc aacttcttga gaacccgtaa acacaatggt
    14461 attcacatca ggatgtttca ccagatgaga acctacgctt gaacctttac aagggacata
    14521 ttgaaagact cccgaaggaa ttcctgcttc gactaaaatt tctgcaaatt tagcagcaat
    14581 caccgatgaa acttccgccg gttttaacaa ggtgcaattt ccagcaacca aggaagcaac
    14641 tgtcatcccc accggaatgg caaaggggaa attccaggga gaaatgacaa cggtaattcc
    14701 cctgggttga taatgatagc ggttggtttc tccggcgaca tcgtaattca ccccgctatt
    14761 gagtcgttcc atttcatcgg cataatagcg acaaaaatca attccctcgg agatttcggc
    14821 atcacattcc cgtaacggtt tgccgacttc taataccatc caggcggata attcatggcg
    14881 gcgttgttcc attaactctc cggctttgcg aagcaagttc gcccgatcgc ctgcgggggt
    14941 ggtccgccat tgggttaagg cggattttgc agcagcgact gcctgatccg cttgttccac
    15001 agacattaac ccaattgtgc caatcacttc cgtaggattg gagggattaa cggagtttac
    15061 ggtattcggt gcattgacgc gatctccatt aattaaaggc agataagttt tacccaattg
    15121 ttggcgaacc gaggctaatg cggctgctgc ttcttcccgt tctttaatct tggcataatc
    15181 gagatcgggt gcagcattgg gtaaggcttt gttgtgaatg gcgatcggtt catctccgtc
    15241 tggttgcgga ggaccaatta aactttcaat cgggcgatct tcctgacttt gccgcacaaa
    15301 tgaactattg gcagtatttt ccaacaagcg gcgaatcaga taagccatcc ccgggagtaa
    15361 atctccataa ggacaataaa cccgcacccg gtatccctgt tgcaccaagg ctttggcgag
    15421 tttatctccc atgccgtaaa gcacttgcat ttcaaagcga cggcgcggga tatttagggt
    15481 ttgggcaatg gcgatcgcat gggcttgcga tcggacatta tgagacccaa ttgccccata
    15541 caaatattcg tgattttcta acagcaactc ggtcatctgt tcaaaattaa catcggttgc
    15601 tgctttgtca ttataaacag gttgcggcca atgtttttga atcgctttaa tggtttcctg
    15661 gtcccaatat gcccctttaa ccaaacgcac tgttaccgga tttccccgtt ctttcgccca
    15721 gtcaatcaat ccttgcaaat cttgtttgct atcgcgtaaa taggcttgca aggtgactcc
    15781 gatatctgtg cgactgcgga attcttcttc catcagcaat cgcttaagaa ttgacagggt
    15841 gaggtcttta taatgatact gttccatatc aaaatggacc gctgcaccca ctgcttgggc
    15901 gtgacgtaag agggtacgaa tgcgatcgat aactcgcgct tcactgccct ccgcatcaat
    15961 cgggtcaaat tgggaataaa atgccgttaa tttgacagaa acctgcactt ggggaatggt
    16021 ttccccatcc gcttcatcaa tttgaggaac tttagaccat tttttggcaa catccgtaag
    16081 ctgagtcatc aaatcgaggt agcgttctag ataagattgc gcctcagctt cggtaattac
    16141 cgcttcccca agcaagtcca ttgtaaaggc cattttgtct tttcgcagac gttcaatggt
    16201 tttgagggct tgcttaatgt tttccccgga aatgtatttt tgcgccaggg tttctatggc
    16261 ggtggatacg gttgtggcgg caatttgacc cggaactgag tcccctccac taaacccaat
    16321 cagttttttg agggcgtcgg ggagttcgac ttcttcgacg gttaaatatt cttgtaagtg
    16381 acgagcaatt tctggtttgc ttcgtaaggc ggggagacag tcaataaagc gaaatagctg
    16441 gacccgtaaa ccggggttgc tcatcgccca atcgagtatt ttatcatccc agcgcatttg
    16501 gtcttgcaat tgggcgaaaa atgaccgctt ttcctgggtt gccgccagga gttgcttggc
    16561 aatttcttga gttttggctt cgtaggttgt gtttttggac gcttgtacta ccacggctga
    16621 ctttaccctt ttttgaggct agatgatggg tcaggtctcc gacgcgatcg cgcctttgac
    16681 tgccaaactc aatattatag ctttttttga agttttgtta aagaataatt ccggtgggaa
    16741 gatgaatgtt tctagcattt gtactcaggt tacctcggaa gatgagccag atttaatgca
    16801 ataaattcgt gataatagtt taaaaatagc agggacgcat tgcttctata gcagtcgggc
    16861 gattctcgaa tcgcccctac agggattggg ttctgtctcg aaataggcgt cagtcctgaa
    16921 aaaaatatca cctcattggt tatagggaca aggtaatatc ttgtcctgac tgggattgaa
    16981 gattattgtt taaagtcccc tagtcagggg gccagaaact gggtttctga ggagaggcga
    17041 tcgcccaaat tgtcaagaat aattacttcc taaaaacctc aaacgggttc cagactctca
    17101 agatccgatt caacttaaat aacactttcc aataattctt taatttcctc taaaatacca
    17161 aatgccgtgt ttagtcctaa atttgtggca ttcattccat ctaccatgag gtctttgctg
    17221 gtcatcactg tggatgtagt agtatccatg acccgactca atcgctccat caattgttct
    17281 atttctttgg ctttatcaat taaattccca taaatttcat ctttaatttg ttctcctaaa
    17341 ttgcttagag tttcttcagc cgattgttcc gtcgtttcag ctaaatcttc catttttgtg
    17401 gtcaactttt ccacaactga attaaagtgt tcgttaaaat cagttgtttt ttgagtgatt
    17461 ttaacgagac attcttcagt tatatagttc tgaagttgtt ccactcgttc ttcgagttct
    17521 gcctgataat ttttgatttc ggcattaact tgatttaaga gatactcttc actttcagaa
    17581 atagcctctt caatttctgc ccgaacggtt tgggtttttt ccgtgagtga actcactgac
    17641 tctgccaatt catcaacttg actttccagg ttgggagcca tgctttctac tttgtcatta
    17701 aactcagaaa tagctccttt cgtagattcg atcgcctctt caatttcttc cggcacaggt
    17761 tcaatggtac ttttagattc ttgaattgca tttttgagac cctctgtctc tgcgataaat
    17821 tgctcttttt cagtcacaat tcgctgaagc aactcattag cttgattgct taaatcttgc
    17881 caattcgctt ctacttcttc tgcgatagta gagagttgtg ctgtcgtttg agcgagacta
    17941 ttttcaattt gtcccgaatg ggtcattaat atctctagct gttcaattgt ttgagtcgcg
    18001 ttactatcta aatcagccat gattatttta atacctgttt cgcttatatc aacatcaaca
    18061 aatgaggaac ttaaattcct ggaatggcat cagcaatact ctgaactaca tcaatcagag
    18121 attctaaagg ttccagtaag gttttcactt gatcgattag aggttcgatg agttgacgct
    18181 cggtcgcact ttcagattta gcatcattaa ggcgatcgtt catcaatccg atcgcttctt
    18241 tgatatgatt gaccgaggac tgcaccatct cttgaatttc tcctttaatt ttttggtcgg
    18301 tctcctggac aaaccgatcc acttctaccg tgaatttatt ttgattgacg gttgtcaggt
    18361 tggtttgcaa attattctgt agggttgtta attgtgtagc catttcttga agatattcag
    18421 tcacttgttc ttgactttct tctaattctg aagtaatcga ctcaacttgt tcttgggttg
    18481 tggactttcc agcttctatt ttctcccgta aaacctcaac ttcgtcactg gtagcattta
    18541 aggcttcctc tagtttgtct cgttgcgctt gtagcaactc tagagtttca ctcaggtctg
    18601 cttgaatttg ttccttagag gtttctaacg cagtcttttg ttctcctaat gcagccttta
    18661 aagattgaac ttggttttga ccttctttga ttaaggcaat cattggagct tgttgattag
    18721 aaatcgtttc ttgaaattga tttaatgttt cttcaagttc ggcgatacct tcagataatt
    18781 cactgggttg actagaaatt gcctgttgta gctcatcgat cgctgtctca atctgagcga
    18841 tcgtcgtagc gatttctgtt tctttagcag tcatgttgct attaaaatca ttgaccgcta
    18901 tttgtaactg tctcgtctct gcaattaagt tgttgagatt ttgaggggcc aaaattaatt
    18961 gattttgcag taaatttagg tccatagttt taggctcaat aaggtaactc agattggtga
    19021 tgtgaattga ctaaaatcaa ctgaatggat tgaacaaatc cttagcggta ttgattgcct
    19081 ggatcacatt tttagcgatg acaagttgtg ggaggatagg actcattgtc gtggtcatcg
    19141 ttgcgccaac cttgaccgtg acgatagttg tcacgatttc ctgaattaat tcttctatga
    19201 cttgattaat cgcctcatca aaggctgaat tgatttcatt ccctaaagaa tcttggcaat
    19261 attcaaccga agaattaatt aactcaattc cttgactctg aaatgaatta cctaaattag
    19321 tgatgtcaga attaaatgtc tcatagagat taatcgcttc agtctcaaaa gtatcaaaat
    19381 ctgtcaccac ctgatttgaa taatcttcag atagagtatt actacagtca gtcatcaaat
    19441 tagttaagcc tgggttatgc tgttcgacaa tatcctcaat caatccctca aaatccgatt
    19501 cagcttcaga tttgaaggta tcgacagttt cttggaattg agcgatcgat tcctcgactt
    19561 cattaaaagt attttgggtt gtttcttgta attctactaa agagtcttcc cagatactac
    19621 tggcttcctc tagttcgaca aaatcatttt ggacttcgct aatattttct gcgagggtct
    19681 gacgatctgc attaaactcc gattgaaaag aattagcagt ggcttctaaa gattctaggg
    19741 agggttctag ttgttcttct gcggctgtgg taaattgcgc tactgattct tgagcagaat
    19801 ttaaacgggt atccgcatca gtccattgct gttgaagatt tcctaagctt tgtcctaagt
    19861 tagcaatgct agtttctaaa agggcaacaa tgtctggcag attttcgctg tccgcttcca
    19921 gttcagaatt cgctgccaca atttgagcat gagatccttc taaaaggctg acaaagttag
    19981 ccagattttc tacgttattt tgaatctcgg cttcaggagc atttactgta gtcatgagtt
    20041 tgattagtta tattggagtt caatgaaata aaagtcataa tctctgtttg tcttcatcct
    20101 tcaagccaac caaccctccc ttaaattaat taatctgtta gacggtttga tttcccacta
    20161 caccagcata tctaccatat ctaaaacggg ttttatggtt tcaataatct gtgtgatttc
    20221 acccactcta tcaataactt ctcccagttt gccatcaaaa atatccgtag ccgactcact
    20281 cgtttcttct aaaaatgaaa tagcattagt taattcagtc gaggccccag ttaattcacc
    20341 aaagacttct tgagtatatt tttgggcaat gccggtcatt acttccaaag aagtagtatc
    20401 taaaatattt tctaatgccg tgagtttatt ctctccttct tccatgagat tgtcaaaggc
    20461 attcataatt gattccaatt gggatttaat ttcagactct aaattatcgt attcttgtaa
    20521 taaggtgact tttttctctt caaaactccc tttagcggtt tctacaattt ctttaaattc
    20581 cctcaaatct ccttgaactg tttctagttc attttctatg tcattcgtca actcttgaac
    20641 ttgttctgac aattgactca attgctctcc gtaactttct gccgctgatt ctaggtcaga
    20701 attttgttgg tcaatttcgc tttcaaactc cgtgagttga gttttgagtt catcggctag
    20761 attagtcgcc tgggtttgac aatttaaaac tctttcttgt agaaccgtca aattttcttg
    20821 agcttgattg atttcatggg ttagggtttc ctctgaagtg gcaatttgct cattcaagac
    20881 ttgaaactga gcttggaaat tcggactagc cgaagtaatc gccccct
//
