LOCUS       NZ_AKXV01000052         6908 bp    DNA     linear   CON 20-MAR-2024
DEFINITION  Streptomyces sp. SS scaffold35_1, whole genome shotgun sequence.
ACCESSION   NZ_AKXV01000052
VERSION     NZ_AKXV01000052.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. SS
  ORGANISM  Streptomyces sp. SS
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 19573)
  AUTHORS   Wang,L., Xie,Y., Li,Q., He,N., Yao,E., Xu,H., Yu,Y., Chen,R. and
            Hong,B.
  TITLE     Draft genome sequence of Streptomyces sp. strain SS, which produces
            a series of uridyl peptide antibiotic sansanmycins
  JOURNAL   J Bacteriol 194 (24), 6988-6989 (2012)
   PUBMED   23209237
REFERENCE   2  (bases 1 to 19573)
  AUTHORS   Wang,L., Xie,Y., Li,Q., He,N., Yao,E., Xu,H., Chen,R. and Hong,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2012) Key Laboratory of Biotechnology of
            Antibiotics of Ministry of Health, Institute of Medicinal
            Biotechnology, Chinese Academy of Medical Sciences and Peking Union
            Medical College, No. 1 Tiantan Xili, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. Sep-6-2010
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000302615.1-RS_2024_03_20
            Annotation Date                   :: 03/20/2024 03:40:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,332
            CDSs (total)                      :: 7,257
            Genes (coding)                    :: 6,818
            CDSs (with protein)               :: 6,818
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 440
            CDSs (without protein)            :: 439
            Pseudo Genes (ambiguous residues) :: 0 of 440
            Pseudo Genes (frameshifted)       :: 293 of 440
            Pseudo Genes (incomplete)         :: 173 of 440
            Pseudo Genes (internal stop)      :: 7 of 440
            Pseudo Genes (multiple problems)  :: 33 of 440
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:58:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6908
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AKXV01000052.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..6908
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..6908
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6908
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[957:1908](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      958..1908
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    36..56
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.18"
                     /tool="antismash"
     gene            complement(160..837)
                     /locus_tag="Y19_RS0114375"
     CDS             complement(160..837)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037149.1"
                     /locus_tag="Y19_RS0114375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_017238444.1"
                     /transl_table=11
                     /translation="MAQQARAIKTRRAILEAAATVFAERGYERTTIGEILVRAGVTKGA
                     LYFHFASKEEVALGVLDAQMLDVPLSPRAIKLQELADQCFLLTHRLQHDALVRASVGLA
                     LDTGATGVDRVGAFQAWIDEVSEILTASQARGELLVHVNVADTARLFCGAFSGVQAMSQ
                     ILCEREDLAHRVSVLLQHFMPSICTPALLTSLDLTEGRVGQLAAEYEAELLRREQNPAD
                     THG"
     misc_feature    877..890
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            958..1908
                     /locus_tag="Y19_RS0114380"
     CDS             958..1908
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037150.1"
                     /locus_tag="Y19_RS0114380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_017238445.1"
                     /sec_met_domain="AfsA (E-value: 1.9e-27, bitscore: 85.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQDGHGATTRVPGEFVHRADPADIIPTGWTQLRADRYSVSAHWP
                     AAHPFFSPVAGDRHDPVLVAETIRQATMLVAHAELGVPVEDQFVMWGLHYTADPEELAV
                     DGLSSDIHLDLVCTELGTRGGRLGNLRTTVVLTRDGRHLATGGALARATSLQAYRRIRG
                     ERMAALGRPVPLIPGVEPHLVGREHAKDVVLAPGVRPGQWRLRVNTGHPTLFRRPNDHI
                     PGMLLLEAARQAAAATLGAGAYLPAGMDVSFARYAELDSPCWIEAETVPTADPSVDTVR
                     VEGRQDDRSVFVATLTSAARPLAVAAGHHAGRLAG"
     gene            1925..2911
                     /locus_tag="Y19_RS0114385"
     CDS             1925..2911
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (5-186). E-value: 2.2e-12.
                     Score: 38.6. Matches aSDomain:
                     nrpspksdomains_Y19_RS0114385_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037151.1"
                     /locus_tag="Y19_RS0114385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_017238446.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.4e-12, bitscore:
                     37.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.1e-10,
                     bitscore: 29.8, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRILITGSTGFIGGRVAATAAARTDVGHVRLLARRPLPPAGVSP
                     ATDRSADERNQDGRPSSETVIGDLRDPASLRRACEGIDVLIHCASAIGGDEESARAVND
                     EGTRALVDAAVRAGATRVVALSTASVHGRGPFRAAAPGTLPLAPASATSRTRAAGERHV
                     LDAGGTVLRPHFVYGTGDRQLVPGLVRLLAALPGPLDAGASLHSVIGVDALAGVLVGAA
                     LSERTRPGPYYVNHPDPVPVADLLAPCEALLAGRAGPPRGPAVGADEARALLADLPFAL
                     HHLAMLVPDHWFEDDRPWRELGCDPGPGYTAGVADHLPWYRGLLDRS"
     aSDomain        1940..2482
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Y19_RS0114385_NAD_binding_4.1"
                     /evalue="2.20E-12"
                     /label="Y19_RS0114385_NAD_binding_4.1"
                     /locus_tag="Y19_RS0114385"
                     /protein_end="186"
                     /protein_start="5"
                     /score="38.6"
                     /tool="antismash"
                     /translation="ITGSTGFIGGRVAATAAARTDVGHVRLLARRPLPPAGVSPATDRS
                     ADERNQDGRPSSETVIGDLRDPASLRRACEGIDVLIHCASAIGGDEESARAVNDEGTRA
                     LVDAAVRAGATRVVALSTASVHGRGPFRAAAPGTLPLAPASATSRTRAAGERHVLDAGG
                     TVLRPHFVYGTGDRQLVP"
     gene            complement(2969..3598)
                     /locus_tag="Y19_RS0114390"
     CDS             complement(2969..3598)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606639.1"
                     /locus_tag="Y19_RS0114390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_037967986.1"
                     /transl_table=11
                     /translation="MTKQERAARTRDALIRSAAGEFDRRGYDLATLSAISSGAGVSPGA
                     LHFHFENKAAVAVAVEYEAAARLRRTARFEYRRPSDALRNLADTTHALARLLREDVVVR
                     AGFRLSCANDLRTELNLRQEWQSCVQQRLAEAADEGLLAFGPERQQDLARTVVAATVGL
                     EALGRDNDEWLSPRTVTGLWRTLLPLLAGPDAFPDPEPADTATPAA"
     gene            complement(3870..4376)
                     /locus_tag="Y19_RS0114395"
     CDS             complement(3870..4376)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513946.1"
                     /locus_tag="Y19_RS0114395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_017238448.1"
                     /transl_table=11
                     /translation="MGVHNVHERLLPAKESEVGALVDALASGEGDRLWPGAAWSPMEFD
                     RPLGPGAVGGHGPVRYTVAGYAPGRWIRFEFTGPRGFHGFHELAVLPVGPDRTRLHHTL
                     TMAPRGLARLTWPLAWRPLHDACLEDGLDRAELACTGTLRRPARWSPYVRLLRTLALAV
                     GRARA"
     misc_feature    4366..4379
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.21"
                     /tool="antismash"
     gene            4440..5021
                     /locus_tag="Y19_RS0114400"
     CDS             4440..5021
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518487.1"
                     /locus_tag="Y19_RS0114400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_017238449.1"
                     /transl_table=11
                     /translation="MARPPRFDTDQLLDAAVRLAASGGPAAVTMSAVAQAVGAPSGSVY
                     HRFAGRAALLAEVWLRTVERFQEGYFAVLTGEPDPRRAARAASRHVVAWCRENPDEAAL
                     LLYGAADFGRADWSDEHARRADSGNNRVFAALAGLAEALGAQDEQARDRIALALVDLPL
                     TVVRRHLRAGTPLPPHAEDLTEHCTAALLG"
     misc_feature    complement(4458..4471)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            5143..5739
                     /locus_tag="Y19_RS0114405"
     CDS             5143..5739
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008414908.1"
                     /locus_tag="Y19_RS0114405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_017238450.1"
                     /transl_table=11
                     /translation="MTRFRESVRSLLREQVLDAAYRLVAADGWGGLRMTAIARAAGISR
                     QTLYNEFGSKEAIGSALVQRELEGFLVGIQRELDAHRGDLEAAAAAGVGYTLQQAVDNP
                     LVKSVLVAARGGEDDLLAYLTTRPEPVFGTAMAMLDAYAIEAWPDVDDESRGLAVETIV
                     RLTVSHIVQPVASPEASARRIARITARIAYPGPAA"
     gene            complement(5702..6094)
                     /locus_tag="Y19_RS38810"
     CDS             complement(5702..6094)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131933.1"
                     /locus_tag="Y19_RS38810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_051034114.1"
                     /transl_table=11
                     /translation="MPSTKTAYVCLPCRASYKQPYGADRRRVCPRCAGELIHVGSAFAA
                     PRRRDAEAWRTLAVLLHAGVRFHKNCCFGGSGYRPRTLREVRERMTYAARTGTPFDQAL
                     VLREPPWPGAVGGPVRRRGPGRRSSR"
     gene            6349..6531
                     /locus_tag="Y19_RS0114415"
     CDS             6349..6531
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037202.1"
                     /locus_tag="Y19_RS0114415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cold shock domain-containing protein"
                     /protein_id="WP_017238452.1"
                     /transl_table=11
                     /translation="MHSFNRLGGYGFVVPVGSEEQVYFSAEDIEGGEKALSEGQQVSFV
                     LVLGEGRFEAKELRV"
     misc_feature    complement(6703..6718)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.79"
                     /tool="antismash"
ORIGIN
        1 cggaaggccg atagaggtcg aagtccttgc ccatgatgtg gtcccctaga cgcttgtggc
       61 actgagtacc ctcagcaaaa cacactcagt gccatgacgc cagagcgccc cgatgccgtt
      121 ctgcatcggg gcgctcaagg aaccggacgg tcggttctgt taaccgtggg tgtcggccgg
      181 gttctgctcc cggcggagca gctccgcctc gtactcggcg gcaagctgcc cgacgcgccc
      241 ttcggtcagg tcgaggctgg tgagcagcgc gggcgtgcag atgctcggca tgaagtgctg
      301 gaggaggacc gagacccggt gcgccaggtc ttcgcgttcg cacaggatct gggacatcgc
      361 ctggaccccg gagaaggcgc cgcagaagag cctggccgtg tccgcgacgt tcacgtgcac
      421 caggagctct ccgcgcgcct gcgaggccgt caggatctcc gacacctcgt cgatccacgc
      481 ctggaacgcg ccgacccggt cgactcccgt ggcgccggtg tcaagggcca gaccgacgct
      541 cgcgcgcacg agggcgtcgt gctgcaggcg gtgggtgagc aggaagcact ggtccgcgag
      601 ctcctggagc ttgatcgccc gcggggacag cggaacgtcg agcatctgcg cgtcgagcac
      661 gccgagggcc acttcttcct tggaagcaaa gtggaagtac agtgccccct tggtcacgcc
      721 ggcgcgcacc aggatttccc cgatggtcgt ccgctcgtaa ccgcgctccg cgaacacggt
      781 cgcggccgcc tccaggatcg cccgtcgggt cttgattgca cgcgcttgct gcgccaaggc
      841 gcctcctgcc gtcggtgctc aacgtgtgac gtgaagcatt gaaaagaaaa ccgggctgta
      901 cgtattctat gcagggcgga cataccgccc gggggcgcgg ggacgcaagg ggtatccatg
      961 gatcaggacg gtcacggagc gacgacgcgc gtccccgggg agttcgtgca ccgcgccgat
     1021 cccgcggaca tcatccccac gggctggacg cagctgcggg ccgaccggta ctccgtctcg
     1081 gctcactggc ctgccgcaca tcccttcttc tctccggtgg ctggggaccg gcacgacccc
     1141 gtgctggtgg ccgagacgat acgtcaggcc accatgctcg tggctcacgc cgagctgggg
     1201 gtacccgtcg aggaccagtt cgtcatgtgg ggcctccact acaccgcgga ccccgaagag
     1261 ctcgccgtgg acggcctctc ctccgacatc cacctcgacc tggtctgcac cgagctcggc
     1321 acccgcggcg gcaggctcgg gaacctgcgc accaccgtgg tgctcacccg cgacggccgg
     1381 cacctcgcca ccggcggcgc cctggcccgc gcgacctccc tccaggccta ccgccggatc
     1441 cgcggcgagc ggatggccgc gctcggcagg cccgtcccgc tgatccccgg cgtggagccg
     1501 cacctggtcg gccgcgagca cgccaaggac gtcgtcctgg cgcccggcgt ccgcccgggc
     1561 cagtggcggc tgcgggtcaa caccggccac cccaccctct tccggcgccc gaacgaccac
     1621 ataccgggga tgctgctcct ggaggccgcc cggcaggcgg ccgcggcgac cctcggggcc
     1681 ggcgcgtacc tgcccgccgg catggacgtc tccttcgcgc gctacgccga actcgacagc
     1741 ccctgctgga tcgaggccga gaccgtaccg accgccgacc cctcggtgga caccgtccgg
     1801 gtcgagggcc ggcaggacga ccggtccgtg ttcgtcgcca ccctcacctc cgcggcccgg
     1861 ccgctggcgg tggccgccgg gcaccacgcc ggccgcctgg cgggctgacc cgacgggaac
     1921 gacggtgcct cgcatcctga tcaccgggtc gaccgggttc atcggcggcc gcgtggcggc
     1981 gacggccgcg gcgcggacgg acgtcgggca cgtcagactg ctcgcccgcc gccccctccc
     2041 gccggcggga gtctcaccgg cgaccgatcg gtccgcagac gagcggaacc aggacggccg
     2101 accttcttcc gagaccgtca tcggcgatct ccgtgacccc gcgtcactcc gccgggcgtg
     2161 cgaggggatc gacgtgctga tccactgcgc ctcggcgatc ggcggggacg aggagtcggc
     2221 ccgcgcggtc aacgacgagg gcacccgggc cctggtcgac gccgccgtgc gcgccggagc
     2281 gacccgcgtc gtcgccctga gcaccgcttc ggtgcacggc cgtggcccct tccgggccgc
     2341 cgcccccggc acgctcccgc tcgcaccggc ctcggcgacg agccgcaccc gcgcggcggg
     2401 cgagcggcac gtcctggacg ccggcggcac cgtcctgcgg ccgcacttcg tgtacggcac
     2461 gggcgaccgc caactggtgc ccggcctggt ccgactgctc gcggcgctgc ccggcccgct
     2521 cgacgcgggc gcctcgctgc attcggtgat cggggtcgac gccctggccg gggttctggt
     2581 cggcgcggcg ctctcggagc ggacgcggcc gggtccgtac tacgtcaacc accccgaccc
     2641 ggtgccggtg gcggacctgc tcgcgccgtg cgaggcgctc ctcgcgggcc gggcgggccc
     2701 gccgcggggg cccgcggtgg gcgccgacga ggcccgcgcg ctgctcgcgg acctcccctt
     2761 cgcgctgcac catctcgcca tgctcgtccc cgaccactgg ttcgaggacg accgtccgtg
     2821 gcgtgagctg ggctgcgatc cgggtccggg ctacaccgcg ggggtcgccg accacctgcc
     2881 ctggtaccgg ggcctcctgg accggtcgtg acctggcctt gctccggaac cgtcaggacc
     2941 ccgcttacga cgggggagtg acgatccgtc aggcggccgg ggtcgcggtg tcggcgggtt
     3001 ccgggtccgg gaacgcgtcg gggccggcga gcaggggcag gagggtccgc cagagtccgg
     3061 tgaccgtccg gggggacagc cactcgtcgt tgtcgcggcc gagggcctcc aggccgacgg
     3121 tggcggccac gacggtccgg gcgaggtcct gttggcgctc ggggccgaag gcgagcaggc
     3181 cctcgtcggc ggcctccgcg agccgctgct gtacgcagct ctgccactcc tggcgcagat
     3241 tcagctccgt acggaggtcg ttcgcgcaac tgagccggaa gcccgcgcgg acgaccacgt
     3301 cctcgcggag cagccgggcc agcgcgtgcg tggtgtcggc gaggttccgc agggcgtcgg
     3361 acggccggcg gtactcgaac cgcgcggtcc ggcgcaggcg ggcggccgcc tcgtactcga
     3421 cggcgacggc gaccgcggcc ttgttctcga agtggaagtg cagcgctccg gggctcactc
     3481 ccgctccgga actgatcgcg gacaaggtgg ccaagtcgta accacgccgg tcgaattcac
     3541 cggccgccga cctgatcagc gcgtcacggg tccgcgcggc ccgctcttgt ttggtcaccc
     3601 gtggctccgt agcaccctgc gtctcgacgg cgcggccccg aaccgcgtcg taccgagacc
     3661 gctcgacaac tccaaaccga ctgcttggta ttgtagaagc tttcccctgt cgcccatgcc
     3721 ccttttggtg atcacgtggg cgggtccggc ggccgagggg ctgccggggc gggcccgcct
     3781 acgagctcgc gccggcggtc gcggccggct gctgcatttc ggagccactc ggcacccagg
     3841 gtttcaaccg gtgcccccgg cagggcggat cacgctctcg ctcggccgac cgcgagcgcc
     3901 agggtgcgga gcaggcgcac atacggcgac cagcgggccg ggcgtcgcaa cgtgccggtg
     3961 caggcgagtt cggcgcggtc gaggccgtcc tccaggcagg cgtcgtgcag cgggcgccag
     4021 gccagcggcc aggtcagtcg ggccaggccg cggggcgcca tggtgagggt gtggtgcagt
     4081 cgcgtgcggt ccgggccgac agggagcacg gccagttcgt ggaagccgtg gaagccgcgg
     4141 ggcccggtga actcgaagcg gatccagcgc ccgggcgcgt acccggccac ggtgtagcgc
     4201 accgggccgt ggccgcccac cgcgcccggc cccagcggac ggtcgaactc catcggtgac
     4261 caggccgcgc cgggccagag ccggtcgccc tccccgctcg ccagggcgtc gacgagcgcg
     4321 cccacctcac tttccttggc gggcagcagg cgctcgtgca cgttgtggac gcccatgcgt
     4381 cctcccctcc agagtattgg agacttctct ccaattaatt ggagggtact ctctgattca
     4441 tggcgagacc accccgcttc gacacggacc agcttctcga cgccgccgtc cgcctcgccg
     4501 cctccggcgg acccgccgcc gtgaccatgt cggccgtcgc ccaggccgtc ggcgcgccca
     4561 gcggctccgt gtaccaccgc ttcgccgggc gcgcggcgct gctcgccgag gtgtggctgc
     4621 gcacggtgga gcgtttccag gagggctact tcgccgtcct gaccggtgag cccgaccccc
     4681 gacgggcggc ccgcgcggcc tcccggcacg tggtggcctg gtgccgcgag aacccggacg
     4741 aggcggccct cctcctctac ggcgcagccg acttcggccg tgccgactgg tccgatgagc
     4801 acgcccggcg cgcggactcc ggcaacaacc gggtgttcgc cgccctggcc ggcctcgcgg
     4861 aagccctggg cgcccaggac gaacaggccc gggaccgcat cgccctggcg ctcgtcgacc
     4921 tccccctgac cgtggtccgc cgccacctcc gcgcgggcac ccccctgccc ccgcacgccg
     4981 aagacctcac cgaacactgc acggcggcac tcctgggcta ggggggtccg ggaaggggga
     5041 gtgcgggccg acggggtcgc gtgacgcccg cccgatccgg gccgtgaggg gtgctctccg
     5101 gcgggggagt gcgccgccgc cggtgactag agtgggcgca cgatgacacg ttttcgcgag
     5161 agtgttcggt ccctgctgcg ggagcaggtg ctcgacgccg cctaccgcct cgtcgccgcc
     5221 gacggctggg gcggcctgcg catgaccgcc atcgcgcggg cggcgggcat cagccgccag
     5281 accctctaca acgagttcgg gtcgaaggag gccatcggca gcgccctcgt ccagcgcgag
     5341 ctggagggct tcctggtcgg catccagcgc gaactcgacg cgcaccgggg cgatctggag
     5401 gccgcggccg ccgcgggtgt cggctacacg ctccagcagg ccgtggacaa ccccctcgtc
     5461 aagagcgtcc tggtcgccgc ccggggcggc gaggacgacc tcctcgccta cctgaccacc
     5521 cgccccgaac cggtcttcgg caccgcgatg gccatgctcg acgcctatgc gatcgaagcc
     5581 tggcccgacg tcgacgacga gtcccgcggc ctcgccgtgg agaccatcgt ccgcctcacc
     5641 gtgagccaca tcgtccagcc ggtcgcctcc cccgaggcct ccgcccgccg catcgcccgc
     5701 atcaccgcga ggatcgccta cccgggcccc gccgcctgac cggtccgccg accgcaccgg
     5761 gccacggcgg ttcgcggagc acgagcgcct ggtcgaaggg ggtgccggtg cgggcggcgt
     5821 acgtcatgcg ctcgcgcacc tcgcgcaggg tccggggccg gtagccggaa ccgccgaagc
     5881 agcagttctt gtggaaccgg acgccggcgt ggagcagcac cgccagcgtc cgccacgcct
     5941 cggcgtcccg ccgcctgggc gcggcgaacg cggagcccac gtggatgagt tcccccgcgc
     6001 agcgggggca gacccgccgc cggtcggcgc cgtacggctg cttgtaggag gcccggcacg
     6061 gcaggcacac gtacgcggtc ttggtggaag gcatgcgggc agcgtaggtg ccgctcgcga
     6121 ggggggccac gggattgtcc ggagcgtcgg tgaggcccgc tgtcggacac gggggatctc
     6181 cggcggcggg cctctgttga agattgtacg aggttgtggg ggggccggtg tccagtcggg
     6241 ggtcgcgggc ccggatggcg gggggtgggt gcgcggggta gatgtcctgg aaggagtgtc
     6301 gccgccgacg acagggaggc cgccgtggaa ggcagaagcg tagggttcgt gcattccttc
     6361 aaccgtctcg gcgggtacgg gttcgtcgtc cccgtgggct ccgaggagca ggtgtacttc
     6421 agcgccgagg acatcgaggg cggggagaag gccctctcgg aggggcagca ggtctcgttc
     6481 gtcctcgtcc tgggggaggg ccgcttcgag gcgaaggagc tgcgcgtctg aggccggcgg
     6541 aggctgccta cgatgggccc atgtctgagc gagtggatcc cccggccgcc tcccgcgcgc
     6601 acgagcggac gccgggcccg cgcgaactct cggcggcggc gggcgtcttc gcgctgctct
     6661 cggacccgac ccggctccat ctcgtgtggc tgctcacccg cggtgaggcc gacgtgaccg
     6721 ccctgacgga ggcctgcggg gccgcccggc ccgcggtgag ccagcacctg gcgaagctgc
     6781 ggctcggcgg gctcgtccag tcgcgcaagg acggccgccg ggtcgtgtac gccatgccgg
     6841 acgggcacct caagcgcctg gtggtcgagg cgatcagccg ggccgaccac gaggtgaccg
     6901 gcgagccc
//
