LOCUS       NZ_JH792253            10357 bp    DNA     linear   CON 15-MAR-2024
DEFINITION  Bacillus mycoides strain VD078 supercont1.3, whole genome shotgun
            sequence.
ACCESSION   NZ_JH792253
VERSION     NZ_JH792253.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 296403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A., Chapman,S.B., Goldberg,J.,
            Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J.,
            McCowen,C., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M.,
            Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S.,
            Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus VD078
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 296403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A., Chapman,S.B., Goldberg,J.,
            Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J.,
            McCowen,C., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M.,
            Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S.,
            Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000290895.1
            Current Name                      :: Bacillus mycoides
            Previous Name                     :: Bacillus cereus VD078
            Date Updated                      :: 2018-06-08
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_000003925.1
            A1 Name                           :: Bacillus mycoides
            A1 ANI                            :: 98.66715%
            A1 Query Coverage                 :: 80.95%
            A1 Subject Coverage               :: 88.35%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_002014665.1
            A2 Name                           :: Bacillus cereus VD078
            A2 ANI                            :: 89.90313%
            A2 Query Coverage                 :: 68.38%
            A2 Subject Coverage               :: 77.02%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R38749
            Assembly Name                     :: Baci_cere_VD078_V1
            Genome Coverage                   :: 146.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000290895.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 02:24:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,225
            CDSs (total)                      :: 6,127
            Genes (coding)                    :: 5,875
            CDSs (with protein)               :: 5,875
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 92 of 252
            Pseudo Genes (incomplete)         :: 158 of 252
            Pseudo Genes (internal stop)      :: 71 of 252
            Pseudo Genes (multiple problems)  :: 58 of 252
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:24:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 184879
            Orig. end                         :: 195236
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JH792253.1.
            The organism was changed from 'Bacillus cereus VD078' to 'Bacillus
            mycoides' in June 2018.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10357
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10357
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10357
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5357](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5357
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(387..>554)
                     /locus_tag="III_RS31880"
                     /pseudo=""
     CDS             complement(387..>554)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016095076.1"
                     /locus_tag="III_RS31880"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VEKRITASQIKKLLEKNMTDTIQGFKSKKTGKSFDAKLTYDSAQK
                     RITFVYEKKK"
     gene            <626..981
                     /locus_tag="III_RS31885"
                     /pseudo=""
     CDS             <626..981
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016514171.1"
                     /locus_tag="III_RS31885"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="DNA repair protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="SVGYSKSYTSGIRKTHTLNPLQT"
     gene            1768..3750
                     /gene="ltrA"
                     /locus_tag="III_RS28855"
                     /old_locus_tag="III_05832"
     CDS             1768..3750
                     /EC_number="2.7.7.49"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ltrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002169891.1"
                     /locus_tag="III_RS28855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_05832"
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_002169891.1"
                     /transl_table=11
                     /translation="MNDKSKPTLKDKKLRHNEYYGIQTVLDDLYQKATKGNSFKKLMPT
                     ITSDENILLAFRNIKGNKGSKTSACDNINIKDIERMNQSYFLNEVKRRFQNYQPQKVRR
                     KEIPKPNGKTRPLGIPSMWDRIIQQCILQVLEPICEAQFCNRSYGFRPNRSAEHAIADS
                     TKKVNQQNLTYVVDVDIKGFFDEVNHAKLMRQIWTLGIRDKQLLVIIRKILKAPVKMPD
                     GTTMFPTKGTPQGGILSPLLANVNLNEFDWWVSKQWETFKAQKVKPRYKDGIWSNDNVT
                     YVLSKTSKMKPMYIVRYADDFKIFTNTRSNAEKIFEACRMWLEERLKLPISTEKSKVTN
                     LKKQESEFLGFSLKATKKGKRKNGNTRYIAETHVSLKALEKTKQDLAKQVKKIQKTPNS
                     IEAIKGINLYNSIVIGKHNYYSIATHVSLDLDKMGLELDCMMYNRFPKAIRRGKSNTNG
                     YTNIGKYEGKDKGIKPYLKSKRMRYLMRRPILPIAYIQHKSPLMKKQAINKYTVEGRAL
                     IHKNLVDITETELKWLRENPVINGQTTVEYNDNRISLYVAQKGKCSVTGEKLLPWGIHC
                     HHKRLWSETKDDSYKNLTIIKPSVHRLIHATKKKVINQLLNELKLNEEQLDKLNKLREL
                     VKNEEICIESQTEVGLNYEQLALFA"
     misc_feature    complement(3808..3820)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.62"
                     /tool="antismash"
     gene            3875..4216
                     /locus_tag="III_RS28860"
                     /old_locus_tag="III_05833"
     CDS             3875..4216
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002203238.1"
                     /locus_tag="III_RS28860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_05833"
                     /product="YolD-like family protein"
                     /protein_id="WP_033713576.1"
                     /transl_table=11
                     /translation="MYNANMPKGRGMVKFTPFAALTAQFDGIREIIKEKNKVTRPILTD
                     EEKELIENMLLCSLLSEEEILITYYEDGYLLSSYMTVVGIDQQHSAVICTDAFYNKMKL
                     QFSNIIDVK"
     gene            complement(4490..>4600)
                     /locus_tag="III_RS34435"
                     /pseudo=""
     CDS             complement(4490..>4600)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898270.1"
                     /locus_tag="III_RS34435"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKLGRAKREGVVGRLIEEGKMKYYKPEGKQRVYVFV"
     gene            5001..5357
                     /locus_tag="III_RS28865"
                     /old_locus_tag="III_05834"
     CDS             5001..5357
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="III_RS28865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_05834"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002169893.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.3e-12,
                     bitscore: 42.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKFLGAGLLAAAILIPAAGASAETGVPTGEPSAWGMVDFDHQIN
                     TIVETSGVDGGKWDHGVTTKLWSNYWHPSKYHRSSVQNGSSKLYRSETEPPDEWSYISI
                     AASGSGNKAWWASF"
     gene            5451..7628
                     /locus_tag="III_RS28870"
                     /old_locus_tag="III_05835"
     CDS             5451..7628
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="III_RS28870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_05835"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_002169894.1"
                     /transl_table=11
                     /translation="MKKIIAALSLVFFLSVNFFIVNYYSINKENELLFKDYIVLSIKYN
                     ESGNKNSFIKELIEISQKNNVNVAQYVNINENQLNIYATNIKNDKHIKLLNGKYPSLNS
                     NEYISNKENDRFKNNVGNIEFPSTNKKISYFNFEQVKNIGLGNMFYINSDDEDSINAVI
                     ELFKRNGTVNISQKSNDPLIPRDINILSLLILTFFCLFTSIIYYTVKQKYSYALKKLFG
                     FSNFHILVKPLKDISFILLGTGILSIIITHIYLRIKHVGFLKSIIGYQSIFLLLIIIII
                     ILCMSALIRIFQKSDLISSLKGNESNKLLRGLLLFGKLIFTMIIFIILGMSLDKQTELK
                     NQLSNLTFWDQTKHIYRLTTSDQGQEQNLSLDRELNNRLVDLYKLLKENKNAFIMDASK
                     YIPLKDTKSETIYAYMSNLKENRPDYGPYGKSVIIDENYLKINPIEFSNGKTIKDHLNS
                     NDDTLNIIVPEKYKSVENEINELYRKYFYMRKVEIYNLNAKSLNIPLSRIQPEDLKVNI
                     VYAKNNQKYFTFDNRLGSIYENHYIKDPIAIIYDGKIDTSSLASYASRCLYFVDNSNET
                     AYQNIEPFLKSTNTNILVSEVQSVYTEVNSRINELKNQNNELETILITLYIITLLISVN
                     YIRLYYYHHSYELYLKTLFGYSFYLRNKVVLYENVLINIVCSISIYTFFNNLAILFAGI
                     VVIILDLIVIFLSSNRLNKQNFNNIIKGGNK"
     gene            7625..8263
                     /locus_tag="III_RS28875"
                     /old_locus_tag="III_05836"
     CDS             7625..8263
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018924060.1"
                     /locus_tag="III_RS28875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_05836"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002169895.1"
                     /transl_table=11
                     /translation="MIELKNVSKAYNSKWIFRNLSLQIDKNEFVTLVGESGSGKTTLLN
                     LMGLLERPDEGSIKVQGLENPQNKDIRKLRRYHFGYIFQNYALLEHDTVKNNILLASKY
                     NEKFQNEMVNEVINTVGLDVGMLQKRVYELSGGEQQRLAIARVLLKPCDIIFADEPTGN
                     LDEQNKFKIIDLLLKLKEAGKTIICVTHDQDVAKSSDRTINIQDLKDKL"
     gene            complement(9106..9744)
                     /locus_tag="III_RS28885"
                     /old_locus_tag="III_05838"
     CDS             complement(9106..9744)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069126.1"
                     /locus_tag="III_RS28885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_05838"
                     /product="nuclease-related domain-containing protein"
                     /protein_id="WP_002169897.1"
                     /transl_table=11
                     /translation="MKVLIFELVLIAILIPLNIMVKKHVPKWKGRAGEKMVTRILSKLN
                     PESYYVLHDVTVHTEYGDTTQIDHIVIAETGVFVIETKNYEGWIYGDEKAARWTQGIFR
                     KKHSFQNPFRQNYKHTKAIEWIMEQQLTCISIVTFHPKCNLKRVHVHSKDKHVLYYNDL
                     QKCIESYTDVQLTNDEVQHIYQTILRVNIIDKDIEKKHVNYLHNKFAKQ"
     gene            complement(9761..10144)
                     /locus_tag="III_RS31900"
                     /old_locus_tag="III_05839"
     CDS             complement(9761..10144)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002204089.1"
                     /locus_tag="III_RS31900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_05839"
                     /product="hypothetical protein"
                     /protein_id="WP_002169898.1"
                     /transl_table=11
                     /translation="MKRNREYIALRNSILVGWLLTLIVMGLSVYFSSIVYLLIGCFILS
                     LKYGLVGTVKNVEKRLPTWANFGVIEKPVNRRVTRPFQFRGGLIVSLALGFVEMVIKGL
                     CISYFILGLETHVYRLMRRNARV"
ORIGIN
        1 cttaatatac aaatgggcta tatgctggat taacttcttt ataatcacaa cacaagcaaa
       61 aacgaaccca ctttggatgt tcgttaaacg tagtcctgaa tgattgcttt ttacatttgg
      121 gacatactga tatttcactc cccttattac gcttatgact ctccacattc tcaaaagata
      181 catctgtaga tggtatttta ctcgtaagca atatatcttc cccctctccc ttttttaggg
      241 atttatagac aacgaaaaaa ggacacacct atggcatatg tatgcgtagg tgtgtccttt
      301 tgcaggtaga tttagaagta ttttgttact ttcatcatat cataattctt attaatttta
      361 tatatgaaat ttaggatttc ataatattat ttcttcttct catatacaaa ggttattctc
      421 ttctgtgcag agtcatatgt taattttgca tcaaacgatt tccccgtttt tttcgattta
      481 aatccttgga ttgtatctgt catattcttt tctaataatt tcttaatctg gcttgctgta
      541 attctttttt caacttaata gaaaacattt cattaaagta attaccacaa aacaaaagaa
      601 gtacctgtta acttaaggac ttatttccgt tggctacagc aagagttata caagtggaat
      661 tagaaaaaca catactttaa accccctaca aacttaacaa tggatatata tcatatctgt
      721 acatactttt tacatcaaca atatactggc gaacccattc gcagcataaa catatcctta
      781 acaaacttac tacaagaagg agaagaacaa atttctcttt tcgataatgt tacccaacga
      841 gaacaagaaa tgaagttaac aaaagtaatg gatgaaatac gcacaaggtt tgggaagaac
      901 agtattttgc gagggatttc ctacacgcat agcgctacag ctagacatag aaacacacta
      961 ataggaggac ataaatcatg agtgcgagac gttatcgact aagcaaaaac gttggtatta
     1021 caacgttttt agcgacaaac gatgactgtt ctttaaagaa ataagccaag gctacaagaa
     1081 caggataact gttcagacaa gtgagaggaa tgaaggagta acgacctgaa actctcccag
     1141 tgagattccc gacagcatac gttaggcata gcgtgatgtt ggaagcaggg taaaacatgc
     1201 aatcattatc cgaagtaccc tataggataa ccgaaagcca ttcggaataa taaaccaaat
     1261 ggaactgata aagtattgta tgctgtcaac cgacatggtg agaatgtgta aacactgacg
     1321 aacttgcgaa tgtacgcctc gatattgtga cgggttagaa atgactcgga gtctgaaaat
     1381 aagacttgtc taatggattg gaataattca tgttgtaaca atcctaacgt tgttataggc
     1441 aacggagcta ggcacggggt atagcaagca cctaaaccgg aggtcatatg acctttaaaa
     1501 caatgatagt ctgaacggaa cgtcgaaagc tactaacgtt gagagaaatc tctatcggcg
     1561 gatatagttg aatgcgaaac ggataaccga gtgaagtatt cttaatagta gtgagagtag
     1621 tggcacgacc cgagaaggat ctgtaatgga actggaggaa cagccacaag cctacattgg
     1681 aggaactgag tttccatcta atcttgcatg gattctggta agactcaaaa ggtcaccaaa
     1741 aatcaatata agagtaggtg atagaccatg aatgacaaat ctaagcccac gctaaaggat
     1801 aagaaactta gacacaatga atactacggt attcaaactg tcctagacga cttataccaa
     1861 aaagcaacca aaggaaattc tttcaagaaa ctgatgccta ctatcacatc agacgaaaat
     1921 atactcctcg cttttcgtaa cataaaagga aataaaggga gtaaaacatc tgcctgtgac
     1981 aacataaaca tcaaggatat tgaaagaatg aatcagagct atttcttgaa tgaagttaaa
     2041 agacgctttc aaaactacca accacaaaag gtaagacgaa aggaaattcc taagcccaac
     2101 ggaaaaacaa gacctctggg aataccaagt atgtgggata gaattatcca acaatgtatt
     2161 cttcaagttt tggagcctat ttgcgaagcg caattttgta atagaagtta tggattccgt
     2221 ccgaaccgaa gtgcagaaca tgccatagca gatagtacta aaaaggtaaa ccaacaaaac
     2281 cttacttatg tggtagacgt ggatattaaa ggatttttcg acgaagtaaa tcacgccaaa
     2341 ctcatgcgtc aaatatggac actgggtata cgggacaaac aattactcgt cattatccga
     2401 aaaatattga aagcaccagt aaaaatgcct gacggtacaa caatgtttcc gacgaaggga
     2461 actccgcaag gaggtatcct cagccctctg ctcgccaatg ttaatcttaa tgaatttgac
     2521 tggtgggtta gtaaacaatg ggaaacattc aaagcccaaa aggtaaagcc tagatacaaa
     2581 gatggaatct ggagtaacga caacgtaaca tacgtcctat ccaaaacatc aaaaatgaaa
     2641 ccaatgtaca tcgtaagata cgcagatgac ttcaaaatct tcacaaacac acgaagtaat
     2701 gcagagaaaa tattcgaagc atgccgaatg tggttagaag aacgattgaa actgcctatc
     2761 tcaaccgaga aatccaaagt taccaatctg aaaaagcaag agagtgaatt cttaggtttc
     2821 tctctcaaag ctacaaagaa aggaaaacgg aaaaatggga acacacgata cattgcagaa
     2881 acacatgtat cactaaaggc actggaaaag acgaagcaag atttagcaaa acaagtgaaa
     2941 aaaatacaga aaacccctaa ctctattgaa gcaatcaaag gaattaattt atacaacagc
     3001 attgtcattg gcaagcataa ctattacagc atagcaacgc atgtcagcct agacctcgat
     3061 aaaatgggtt tagaactcga ctgcatgatg tataaccgtt tccctaaggc aataagaaga
     3121 ggaaaaagca acactaatgg atacacgaat ataggaaaat atgaaggaaa agacaaaggt
     3181 ataaaaccct atctaaagtc aaaaaggatg aggtatctca tgagacgtcc tatcctacca
     3241 atcgcctata ttcaacacaa aagtccgttg atgaaaaagc aagccattaa caagtacacc
     3301 gtagaaggac gagccttgat acacaaaaac ttggtagaca taactgaaac agaacttaaa
     3361 tggctaagag aaaatccagt cataaatggg cagacaaccg ttgaatacaa tgacaatcga
     3421 atctctcttt acgtagctca aaaaggtaaa tgtagtgtga caggcgagaa acttctgccc
     3481 tggggtatcc attgtcacca taagcgatta tggagtgaaa caaaggatga cagctacaaa
     3541 aaccttacca tcatcaaacc aagtgttcat agactaatac acgcaaccaa gaaaaaagtc
     3601 ataaatcaac tactcaatga acttaaactc aatgaggaac agctagacaa acttaacaaa
     3661 ttgcgagagc tagtcaaaaa cgaggaaatc tgtattgaat cacaaactga agtgggatta
     3721 aattatgaac aactagcgtt gtttgcttag aatttatcat tattagatgg aaagaacact
     3781 tacaatatag gtggaccgcg gtatgccgtg aaagtggcac gtaccgtgcg gatcggggga
     3841 aaagttagag ataacttcaa aaacttacct atcgatatat aacgctaata tgccaaaagg
     3901 gcgcggtatg gtcaaattca ctccgttcgc agcattaaca gcacaatttg acggcatacg
     3961 tgaaatcatt aaggagaaaa acaaagtaac tcggccgatc ttaacagatg aagaaaaaga
     4021 attgatcgag aacatgttgc tatgttcctt attatctgaa gaagaaatac taattacata
     4081 ttatgaggat ggttatttac tctctagcta catgactgtt gttggtattg accagcaaca
     4141 tagtgctgtc atatgtactg atgcttttta taacaagatg aagctgcaat tttcgaatat
     4201 aattgacgta aagtaaaata taatcctgtg taaagccgac ccttaaagga agaggcttta
     4261 cacaggatta tataatcgac aaacaaatgt tttgtataga tttatttcac tttatacaat
     4321 tctgaatgta ttactttttc atcatctatg ttaaattttc taagtatcac ttttaccaca
     4381 tctacaagcc atggagctgc atcaggagaa acatatacac gttcaattaa tttagtaaga
     4441 tcgacttgaa cacttatcct ttatatctga attgactccc ttgatctctt taaacaaata
     4501 catacaccct ttgtttcccc tccggtttat agtatttcat tttcccctct tcaatcaatc
     4561 tacccactac cccctccctt ttagctctcc ctaatttcat ctagcataaa taacatttct
     4621 atcacctctc gttacttata aataacaaac actttaatta taagtttctt ttgtttatgg
     4681 caaacttata attaaagtga catttacata ggaaataagt agataaaaag ctgacagttc
     4741 atcccactct acaaaaacag aataatcagt ttttttgttt ctcaagaatg atacttcctt
     4801 gaatttccgc taacatgata agattatgga gatatattat ctgtgtcctt catgtatttt
     4861 cactagaatt attttgggat tctcacagaa aaaatattta acgtattttt ctaaatttac
     4921 ataatattat ttacatttac atattttgtg atatataatt aatttgctag tttattacca
     4981 agagaaggga gaaaaggatt atgaaaaaat ttttaggcgc aggtttacta gcagctgcaa
     5041 tcttaatacc ggctgctggg gcatcagcgg aaacaggggt acctacagga gaaccatctg
     5101 catgggggat ggtagatttc gatcaccaaa ttaacactat agtagaaacg agtggtgtag
     5161 acggtgggaa atgggatcat ggtgtaacaa ctaaattatg gtctaactat tggcacccat
     5221 ctaaatacca tagatcatct gtacaaaacg gtagtagtaa gttgtataga agtgagacag
     5281 aaccacctga tgagtggtca tatatatcta ttgctgcttc cggatctgga aataaagcat
     5341 ggtgggcaag cttctagtcc ttaatatttt tataaaatcc cgctaaataa attagcggga
     5401 ttttataaat catatgtaca ttaaaattgg aactaaaagg ggggattgga atgaaaaaaa
     5461 tcattgctgc tctatcattg gtattttttt taagtgttaa cttttttatt gtaaattatt
     5521 attctataaa taaggaaaat gaattgctgt ttaaggatta tattgttcta tctataaaat
     5581 ataacgaaag tggcaataaa aattcattta taaaggaatt aatagagata tcacagaaaa
     5641 acaatgtaaa tgtagctcaa tatgtcaata ttaatgagaa tcaacttaat atctatgcta
     5701 cgaatattaa gaatgataaa catataaagt tactaaacgg aaaatatcca tctttaaatt
     5761 caaatgagta tataagtaat aaagaaaatg atagatttaa aaataatgtg ggaaacatcg
     5821 aatttcctag tacaaataaa aagatttctt attttaattt tgaacaagtt aaaaatatag
     5881 gtttaggtaa catgttttat ataaactcgg acgatgaaga ttctattaac gcagtaattg
     5941 aattattcaa acgaaacggc actgttaaca ttagtcaaaa aagtaacgat cctcttattc
     6001 ctagagatat taatatttta tcactattaa tacttacttt tttctgtctg ttcacgtcta
     6061 taatttatta caccgttaag caaaaatact cttatgcatt gaaaaaatta ttcggatttt
     6121 ccaattttca tatacttgtt aaaccgttaa aggatattag ttttattctt ttaggaacag
     6181 gaatattatc aataattatc acacatatat atttaagaat taaacatgtt ggtttcttaa
     6241 aaagtattat cggatatcaa agtattttct tactattaat tataatcata attatattat
     6301 gtatgtccgc tcttataaga attttccaaa aatccgatct tatttcctca ctaaaaggga
     6361 atgaatcaaa taaattatta agaggattat tactatttgg taaattgatt tttactatga
     6421 ttatatttat tattctaggg atgtctttag acaaacagac tgaactaaaa aatcaattaa
     6481 gtaatttaac tttttgggat caaacaaaac atatttatcg ccttacaact tcagatcagg
     6541 gtcaggaaca aaacctatca ttagatcgtg aattaaataa taggttagtg gatttgtata
     6601 aattattaaa agagaataaa aatgcattta ttatggacgc atccaaatat attcctttaa
     6661 aggacacaaa atctgagacc atatatgctt acatgtctaa tcttaaagaa aacaggcctg
     6721 actatggacc atatggaaag agtgtcataa tagatgaaaa ttatctaaaa ataaatccta
     6781 tagaattcag taacgggaaa actattaagg atcatttgaa ttctaatgac gacacgttaa
     6841 atattatagt tcctgaaaaa tataaatcgg tagaaaatga aataaatgag ttatatcgca
     6901 agtattttta tatgagaaaa gttgaaatat ataatctaaa cgcaaaatca ttgaacattc
     6961 cattatcaag aattcaacct gaggatttaa aagtaaatat agtttatgct aaaaacaatc
     7021 aaaagtactt tacttttgat aatagattag gaagtatata tgaaaatcac tacataaaag
     7081 atccaattgc catcatctat gatgggaaaa tagatacatc atctctagca agttatgcat
     7141 ctagatgttt atactttgtt gataattcaa atgaaactgc ctatcaaaat attgaacctt
     7201 ttttaaaatc tacaaacacc aatattttag taagtgaagt tcaatcagta tatacagaag
     7261 ttaacagtag aataaacgag ttaaaaaatc aaaataatga attggaaaca attttgataa
     7321 ctttgtatat aatcacttta ttaatctcag ttaattatat acgtctctac tactatcatc
     7381 attcatatga gttatattta aagacattgt ttgggtattc tttttatttg cgtaacaaag
     7441 tggttctcta tgaaaatgta ttaattaata tagtatgttc aattagtata tacacgtttt
     7501 tcaataatct agctatattg tttgctggga tcgtagtaat catattggat ttaattgtta
     7561 tatttttatc tagcaaccgc ttaaataaac aaaattttaa caacataata aaggggggga
     7621 ataaatgatt gaactaaaga atgttagcaa agcatataac tcaaaatgga ttttcagaaa
     7681 cctttctcta cagatagaca aaaatgaatt tgtaactctt gttggagaga gtggtagcgg
     7741 gaaaactacg ctattaaatc ttatggggtt attagaaaga ccggatgaag gctcaattaa
     7801 ggttcaggga ttggaaaatc cccaaaataa agatatacgg aaattgagaa ggtatcattt
     7861 tggatatata tttcaaaatt acgcattatt ggaacacgat actgtaaaaa acaatatact
     7921 tttggcatca aaatataacg aaaaattcca aaatgaaatg gtaaatgaag taataaacac
     7981 tgtaggttta gatgttggaa tgttacaaaa gagagtttat gaattgagtg gaggagagca
     8041 acaaaggtta gcaattgcta gagttttgtt aaagccctgt gatattattt ttgcagatga
     8101 acccacaggt aatctagatg aacagaataa atttaaaatt attgacttgt tactaaaatt
     8161 aaaagaagcc ggaaaaacaa taatctgtgt tacccatgat caggatgttg caaagtcttc
     8221 tgatagaact attaatatac aagatttgaa agacaaacta tagaagcttg tatccaaact
     8281 ttaatgattt tataattaga tatgatttac ttgagaaagg gggcgatttt ttgaaacaag
     8341 ttttccttaa ttgtattcta attatcatca ctataatttc ccaaaaggtt atatttaggg
     8401 tatttgcact cccctataat aattggctat taaattgggg tactttccta ttaatattta
     8461 tttttattaa ttcaataacg ataattttta gtaagaaaaa taaaagttta agagtaaatt
     8521 gatgccttta atttttgtat aaataaattg aaaaatactt ttttcagaaa atgagttatt
     8581 ccaaaaaaaa ttatgcaaaa caaaaaggaa tccatagttg attccttttt atattcccaa
     8641 aaggtacacc ttttgttgtg gtgtgatttc tatgaaaaac aatgtgtcaa aaagttcgag
     8701 ataacatttt gcagtacttc ataaatacat agaccttttg aattattgta aattagtatc
     8761 ccaaaaccga ttattttggg gacaatcaaa tatcgagagg tttttgatgt tattttgtcg
     8821 aaaaacttcc caatacaata aaatgtaaat tttcacgttt tgggggtatt tacatttctt
     8881 agcttgatag cgttgtggtg gtatcccctt atgcgaatac taaagtccat ttcaattgaa
     8941 atggacttta aaacatttta tcagaaatct tctactcttt ttttttcatt acaaaatctc
     9001 tcggattccc tctaagcttg taatacttca tgttaagacg aatagcaacc aaagaaatta
     9061 taataaatcc tatgatataa ggaatcatgt aacctactcc atttcttatt gtttagcgaa
     9121 cttattatgt aaatagttca catgtttctt ttctatatcc ttatcgataa tgtttactcg
     9181 caatatagtt tgatagatat gctggacctc atcatttgtt agttgcacgt cagtatatga
     9241 ttcaatacac ttttgtagat cattataata taggacatgt ttatctttag aatgaacatg
     9301 tacccttttt agattgcatt ttggatgaaa cgtaacaata gagatacatg tcagctgttg
     9361 ctccataatc cattcaattg ctttcgtatg tttatagttt tgtcgaaatg gattttgaaa
     9421 agagtgtttc tttctaaaaa taccttgtgt ccatcttgcc gccttctcat ctccatagat
     9481 ccagccctcg tagttcttcg tctctatgac aaatacacct gtttccgcga taacaatatg
     9541 gtcaatttgc gttgtatcac catactccgt atgaaccgtc acatcatgta acacataata
     9601 actttcggga tttaatttgc ttaagatgcg tgtcaccatt ttttctccgg ctcttccttt
     9661 ccattttggt acatgttttt ttaccatgat attcaacgga atcagaatcg caatgagaac
     9721 taattcaaat attaatactt tcataatata agcctccctt tcacactctt gcgtttctgc
     9781 gcattaaacg atacacatga gtttctaatc ctaaaataaa atatgagata cataggcctt
     9841 ttatcaccat ttctacaaat cccaatgcta atgaaacaat aagcccaccc cgaaattgaa
     9901 agggtcttgt cactcttcga tttactggtt tttcaataac accaaaatta gcccaggtag
     9961 gtaaacgctt ttccacattc tttacggtac ccactaatcc atatttaagc gacagaatga
    10021 aacatccgat taataaatat acaatggaag aaaaatagac agataggccc ataacgataa
    10081 gtgtgagcaa ccaacctacc aaaatggagt ttcgcaaagc aatatattct cgatttcttt
    10141 tcaaaacaaa cagtcctttc aaaataaata aggttatttt tgtttgggaa atatttacat
    10201 ttaattataa gcatgatatg atacgtctat attaatttac gaattatcct aaaaaggatt
    10261 gtgatagaca tgttaaaact tgtagtagat aatacggtaa cagaacaaga aacagattca
    10321 gctttttcaa accttttcac atgcaaagac tgtgtat
//
