LOCUS       NZ_JH792251            10276 bp    DNA     linear   CON 15-MAR-2024
DEFINITION  Bacillus mycoides strain VD078 supercont1.1, whole genome shotgun
            sequence.
ACCESSION   NZ_JH792251
VERSION     NZ_JH792251.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5172041)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A., Chapman,S.B., Goldberg,J.,
            Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J.,
            McCowen,C., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M.,
            Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S.,
            Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus VD078
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5172041)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A., Chapman,S.B., Goldberg,J.,
            Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J.,
            McCowen,C., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M.,
            Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S.,
            Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000290895.1
            Current Name                      :: Bacillus mycoides
            Previous Name                     :: Bacillus cereus VD078
            Date Updated                      :: 2018-06-08
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_000003925.1
            A1 Name                           :: Bacillus mycoides
            A1 ANI                            :: 98.66715%
            A1 Query Coverage                 :: 80.95%
            A1 Subject Coverage               :: 88.35%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_002014665.1
            A2 Name                           :: Bacillus cereus VD078
            A2 ANI                            :: 89.90313%
            A2 Query Coverage                 :: 68.38%
            A2 Subject Coverage               :: 77.02%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R38749
            Assembly Name                     :: Baci_cere_VD078_V1
            Genome Coverage                   :: 146.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000290895.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 02:24:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,225
            CDSs (total)                      :: 6,127
            Genes (coding)                    :: 5,875
            CDSs (with protein)               :: 5,875
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 92 of 252
            Pseudo Genes (incomplete)         :: 158 of 252
            Pseudo Genes (internal stop)      :: 71 of 252
            Pseudo Genes (multiple problems)  :: 58 of 252
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:24:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2734578
            Orig. end                         :: 2744854
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JH792251.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The organism was changed from 'Bacillus cereus VD078' to 'Bacillus
            mycoides' in June 2018.
FEATURES             Location/Qualifiers
     region          1..10276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5276](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5276
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            569..1480
                     /locus_tag="III_RS14010"
                     /old_locus_tag="III_02820"
     CDS             569..1480
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="III_RS14010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02820"
                     /product="DMT family transporter"
                     /protein_id="WP_002168286.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYTPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASVFLNERMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYQEIVTAPIEITLSVIYLGV
                     FPTVLPYIALAYITSHASASEATSSLYLTPVTACIIASIWLGEVPTFVSIIGGGITILG
                     VVIAHLTFNKDDYKQIESEKVW"
     misc_feature    complement(1498..1514)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            1764..2687
                     /locus_tag="III_RS14015"
                     /old_locus_tag="III_02821"
     CDS             1764..2687
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="III_RS14015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02821"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_002127616.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNELMLNIKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTYILVNTQNPFKSIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     gene            2765..3229
                     /locus_tag="III_RS14020"
                     /old_locus_tag="III_02822"
     CDS             2765..3229
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="III_RS14020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02822"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_002168287.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLRVKENQIHLVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            3413..3982
                     /locus_tag="III_RS14025"
                     /old_locus_tag="III_02823"
     CDS             3413..3982
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="III_RS14025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02823"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_000665071.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKDRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFGKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            4355..4801
                     /locus_tag="III_RS14030"
                     /pseudo=""
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="III_RS14030"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIWESAENA
                     LIRELREELGVTYSIK"
     misc_feature    complement(4972..4987)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            5001..5276
                     /locus_tag="III_RS14035"
                     /old_locus_tag="III_02825"
     CDS             5001..5276
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="III_RS14035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02825"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001001869.1"
                     /sec_met_domain="TIGR03601 (E-value: 7.3e-39, bitscore:
                     130.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     gene            5390..6418
                     /locus_tag="III_RS14040"
                     /old_locus_tag="III_02826"
     CDS             5390..6418
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="III_RS14040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02826"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_002127614.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVESIKETNQSSAAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     TPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            6450..6593
                     /locus_tag="III_RS33560"
                     /old_locus_tag="III_02827"
     CDS             6450..6593
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="III_RS33560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02827"
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            6872..8080
                     /locus_tag="III_RS14050"
                     /old_locus_tag="III_02828"
     CDS             6872..8080
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="III_RS14050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02828"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_002168289.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTVTETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            8261..8677
                     /locus_tag="III_RS14055"
                     /old_locus_tag="III_02829"
     CDS             8261..8677
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="III_RS14055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02829"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_002085307.1"
                     /transl_table=11
                     /translation="MQIVKQWVQEDSDYIREKVIEYNQKQLSNEEKTPSEKISFIVKNE
                     KEEIVGGITAITFWHHVYVDFLWVSEEYRHEGYGSNLMNLIEEFAIKKECRLIKLDTFS
                     FQAPNFYKKHGYKVIGVSEDYPKGHNHYYLEKRL"
     gene            8862..9305
                     /locus_tag="III_RS14060"
                     /old_locus_tag="III_02830"
     CDS             8862..9305
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="III_RS14060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="III_02830"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002168290.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFKERGHHAIFFNFLGEITGGEICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     misc_feature    complement(10244..10258)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
ORIGIN
        1 cgcataatga attgcatagt ttctaacggg aaaagatgag gtgcaagttc tcctttacta
       61 agatgaatca attttttatt tgcttctgct tcatttattt ttcgtaccat taacttgctt
      121 ggtttatgaa taccagcctt tacatattca ctccaatcgg gcgggggatt ggtggctaat
      181 aatgaccatg tattattcgt tacaattgtt cctgacccga ttttcccttg aattaagcca
      241 tctgccataa gttcttctag tgcaattatt acagtactac gatttacatg aaataatttc
      301 gctaactgcc gctggctagg aaccttactt ccaatcggcc attctccgta ttcaattttt
      361 tctttcatat agtctattat ctgccgatat ttaggcagtt ttttatcagt aaacatatta
      421 aaaactccct cattcatttt aaaaatgcaa agtggttggt tttttcgagg attaactggt
      481 tgaagacatt gtatcaacta ttaaatatga tgaaaagaaa taattggtaa ttagaaaatg
      541 gctggtttat tggagaggga gagaatatat gcataataaa aaatgggatt ttcgtattgt
      601 atgtgctcac gcttttacga ttcttatatg gggaacagct tttccaggaa ttcggatggg
      661 gcttgaagct tacacccctg aacaccttac tttgctacga ttattaatcg cttctttgat
      721 tctttttctg ttttcactta tatacaagtt acgtttacct gatttaaaag atattccagt
      781 aatttttata tttggtgctc ttggatttac aatttatcat attgcactga actacggtga
      841 aaagagtgtg aatgctggac ctgctagttt aattgtatca gtaacaccta tattaacggc
      901 aattcttgct tctgtttttt tgaatgaaag aatgaaatta aatggttgga tcggtggtgt
      961 aattagtttt gtaggaattg cttttttatc gttaagtcaa ggagattcca ttcaattgaa
     1021 tagtggggga ttactaatat tattggcggc aatttcagaa agtctatttt tcgtttttca
     1081 aaggccatac ttaaaaaagt acggtttctt gccatttaca atatatacaa tttggtctgg
     1141 tactgtattt atgcttattt ttttgccagg gatttatcaa gaaatcgtaa cagctcctat
     1201 agaaattact ttgagtgtta tatatttagg tgtgttcccg acagtactgc cgtatattgc
     1261 attagcatat attacatccc atgccagtgc ttctgaggca acaagttctc tatatttaac
     1321 accagtaact gcatgtatta tcgcttcgat atggttaggt gaggtgccaa cctttgtttc
     1381 gataattgga gggggaatta ctatacttgg agttgtgatt gctcatttaa catttaataa
     1441 agatgattat aagcaaattg agagtgaaaa ggtatggtaa gacatcccta cgagtatttg
     1501 caatttgaat gcaaaatttc ggattaataa caggttttca attctataaa tatagtgaaa
     1561 aaaatctata ttaaaatcag ttagtccaat atttgtacga gatttaatta aactggtaga
     1621 ttatttatct gcaaaagaac tagcataatg ctagttcttt tttatcaata cattactcac
     1681 cattaatatt tgcttagaac tgtatagatt taattttttt ccacatatac ataattatct
     1741 tacctatgaa aggagtgcat ttcttggaga aggttttatt ttttggagac ccaggtattg
     1801 atgattcgtt tgcaattatg tatggcttac tgcatcctga aattgaaatt gttggtatcg
     1861 taactggata tggaaatgtt gaacatatac atgccgctca taatgccgca tatattttac
     1921 agttagcaaa cagacaagat attcctgtaa ttagcggtgc aacaggccca cttacaaatg
     1981 aaattacaac gtattaccct gaaatccatg gaccagaagg attagggccc atccatgtac
     2041 cggaaagtgt gttatctatt cctatataca atttcggtag tattgctgcg attcttgtaa
     2101 agtatggaga agatttaaca attgtcgatg taggaagatc tacctcgtta gcgattgctt
     2161 ttaatttatg gaacgaattg atgttaaata taaaaggaat ttattttatg ggtggtgtct
     2221 ttttagaagc agggaatgtt actccattag cagaggcaaa cgtgtacgga gatcctatag
     2281 caagtcaaat tgtatttcag caagcgaaaa atataacgtt attcccttta aatgtaacaa
     2341 ataaaacggt tttaacccca agtgtattta cttacatttt agttaataca caaaatccat
     2401 ttaagtcgat tatgaaaccc atgtacgatt attatttatc agcataccaa aaactcaatc
     2461 catctattga agggccatta ttacacgatg tacttgctat aagcggttta gtgaatccta
     2521 catttttaaa ttatacatcc cgaaaagtga ctgtggatat atgtggcgaa acgaagggac
     2581 aaacttttgc tgattttcgc ccacactcta aatctgaagg ggcgcgtata gcgttacaat
     2641 tagatgagga aaaatttatt caggatttta tgaaaatcat gttataagtt gatgtcgaaa
     2701 aagggaatca tttcactccg ttgaatataa aagataaatt ctctttttaa aggagcttaa
     2761 aatagtgaac gttcaattaa aaattgttac gagagaaaat tgggaagacg cattaaagtt
     2821 acgagttaaa gaaaatcaaa tccatcttgt tcccgcagtg gcagtatcac ttgctaaagt
     2881 atacataaaa ccagatggtg aaaatgtcga atatattcca ttcgctatat atgatggtga
     2941 ccttatagtt ggttttatta tgcatgcttt tgtacgagag tcaacagata tgtattggat
     3001 taacggattt atcattgatg aaaaagagca aggaaaagga tatggaaaag cagcattaca
     3061 ggaaatcatt aacttaataa aaaataactt taaagagtgt gaggaaatta gattaactgt
     3121 ccataaagat aatatttctg caaagaaact atatgaatgt tatgatttta aaccattagg
     3181 aaatgtatat gacggtgagg aagtatatcg cttattgttg ttgaaataaa gatgtactgt
     3241 tttgaaacaa taagttgttc cgttaaaggg tcatattgcg gagaattggt tgtactaatt
     3301 catataaatt atcgcaaact caattcacgc aattatttaa acattttatt ataaaacagt
     3361 atttatatat acttaaacta tcattaataa aaacatcaat ggaggaacta atatgaaaga
     3421 aagcaataaa aaagagttgt cactagagca acgtgaagaa ttactacaaa cattgaaaga
     3481 tcgttttgag aataatatga accgtcataa aggtttagaa tgggctaatg ttcaagaaag
     3541 gctggatgct aatactgaaa agttgtggtc gctcaacgaa atggaacgaa ctggtggcga
     3601 acctgatgtt gttgattttg gtaaagaaaa aggcgaatat attttttatg attgttcagc
     3661 agaaagtcct aaaggtcgta gaagtgtttg ttacgatctt gaagcgctag aatcaagaaa
     3721 gaaacataaa ccagaaaata acgctattga tatggcaact gccatgggta ttgaactatt
     3781 aacggaagaa gaatatcgta cattacataa tcttgaaaat ttcgatatga agacgtcgag
     3841 ttgggtgcaa acaccttctg atattagaga acttggcggt gccctttttt gcgattatcg
     3901 ctttggacat gtctttgtgt atcacaatgg agcagaatcc tactatgccg caagaggttt
     3961 tcgtggttcg ttaagagtct aaaatttgta caggcaggta aatttgaagt agagaataga
     4021 tttgcttagg gaatagtagt gattgcatac tttagctacg gcttcgattt tgaggccgca
     4081 aagaataggg ctaatcaaac cttattgtaa aagaatgaat caactaaaaa tattaagagt
     4141 atgttttgat aattttcttc ataaacatac tcttttttag aatcatttat caaaattatt
     4201 agttttaatt aacgtataaa cgagaacttg taaaaggtac tcttacaatt atcaatgaaa
     4261 ttcacccata tgaaacacct atgatttata ttatcccgct tttaaatgaa aattttcgag
     4321 caagtataaa gtgaatgagt aggaggaata caaaatgaaa agtaaattcc atcatattgt
     4381 acgagctatc atgataaaag atgaaaaatt actagttgct gaatacatag gccatcacta
     4441 ttttttaccc ggtggtcatg ttgaaatttg ggaatcagca gagaatgcac taataagaga
     4501 gttacgagaa gaacttggag taacttacag tataaaataa tttttaggcg tcatagaaaa
     4561 tcaatggcaa gccaaagaaa ccctccacca tgaaattaac cacatttttg aaatagattc
     4621 aaatgagtta aaatccgatt tcactccaat atctaaagag tcccatttag catttcactg
     4681 gattgatttt aataaagaaa atataaattc ctatacaata atgccgacac cttcagtaaa
     4741 agaattacta gaaaaaaaat taagtgatga actattaact tgctggatta gtaattttta
     4801 attgtctcta tttatattaa ttccgtccac taaaagaata taaagaaaat aggctcataa
     4861 acaaggtaca tcacctagtg taaagtcata ttttgttgat gtacgcattt agtctatagc
     4921 cttatttcta ctttattttt aaaagtaaat aaactcgaaa atttggacgg aatttacatt
     4981 tgtaaatgga gggtttcata atgaatgagt ttcaacaaga actacaagcg ttaaacctta
     5041 atgcttatca acctggtaat gttgtgtatt gggatcagca acaaaatcaa tatccatatt
     5101 actacattca agacgatgca cgtcgttgtg gcggatgcgg tggcggcgga cgttgtggcg
     5161 gttgtggtgg tcgctgtggt ggttgtggcg gcggacgttg tggcggttgc gtaggttgtg
     5221 taggctgtgt tggttgtttc agctgttcta attgctggaa ctggtggttt atttaagtat
     5281 tatagatcta taatatttca catgaaagaa aagtgcagat gcaaagttat aatgtattga
     5341 aacttttctt tcatgctgtt atttttatat gttttagggg gatatttatt tgtacaaata
     5401 tgaaaaatta atttcatggg tagaaagcat taaagagacc aatcaaagtt ctgcggcagc
     5461 actttgtatt attaaagata ataagattgt actagagcat tatagtggaa cgcattcaaa
     5521 tactcctact agtaaaaaag taactatatc ttcacaattc aatgtcgctt ccgcaagaaa
     5581 aagctattta ggattaatgg tagcttatgc agtttatgag gggaaaataa aatctcttga
     5641 tgacgaggct gtaaattatt ttaaagaata tgatccttca ttacttggaa agacaacgat
     5701 aagacattta gtaactcatt cacacggttt aaatgaaacc gatgatggga cagcttttcg
     5761 tgaatttgag ccgggacaag cttgggcgta cagagatatt aatgtaagaa tggtgacgca
     5821 gcttatttat caactgtata ataagagttt tcctgaacta ttaaaggagc gtgtattcac
     5881 acctgctgat ttcaaaaaaa ctggatggag aataaaacaa gatgataatt tagttaaggt
     5941 tgttgtaaac ccagatgaag atgcaataag tagtgttggt gcagtagatg acggctccga
     6001 gaaaaatctt tttgtctctg cgagagaatt tgcattttgg ggtaaccttc atttaaataa
     6061 agggttttta aatggtaaac aaattgtgcc aaaagaagtt atagcacttg ctacgagctt
     6121 acaaagtcca gagtatacaa ataaagaact acctcaaaac gggttgtttt ggttcgttca
     6181 aaatgaacct gctttattta gcgaacttgg tgagcgagtt cctaaaggtt cttatcaaat
     6241 attaggaatt acaggaccaa caatattagt cattccagaa tataatgtcg tcgttgctaa
     6301 aatgtacaat aaaagataca actatggtgg ggataattat ttatattatt tacgtgaatt
     6361 tagcaattta gtcgctgata catttagtag cagtaaaaat actatggtta gggtataact
     6421 actcctataa taaaaaggag tgaactttaa tgaaaaatga agtgaataaa aataaaaagc
     6481 aaaatataaa ggatgtaaac acagaacaaa acgcgattta tagcgatccg aaagatgcag
     6541 ctaatatgca aactgtaccg cagccgaaag actttgagga aattgaatat taattttctc
     6601 tccgacttat ttatgaaaga aaaatacgtc ttaagtctca tcactttcag caaattattt
     6661 gataacatat atacataagc attcgaaaat gtcttatctt aacaaagctt tctttaaatg
     6721 ctgtatataa cctacaaaaa ggcacttctt gatgaagtgt ctttttgtgt aagctaactc
     6781 aatttccatt ttccgtacag tatcttgcaa tttatcatgg gtatactaac tgtgaaaatt
     6841 catgtcatga taaaagaagg ggacagttag catgaaacaa acatctataa atccattgct
     6901 aattgttcta ggtacaatca ttgttcaaat tggcctagga acaatttaca catggagttt
     6961 atttaatcaa ccccttgtaa gtaagtttgg atggaacctc aattcagtcg cgatcacgtt
     7021 ctcaattacg agtttttctt tatcattctc aactctattt gcaggaaagc tacaacaaaa
     7081 atttggactt cgtaaactta tcgctactgc aggaattgtt ttaggactcg gtttaatact
     7141 tagttcacaa gtttcctcat taccattact ttatttatta gccggtgttg tcgttggata
     7201 cgctgatgga acagcatata ttacatcact atctaattta attaaatggt ttccaaaccg
     7261 taaaggactt atttctggta tatctgtttc ggcatatgga atgggaagct taatctttag
     7321 atatataaac ggaaatctta tcaacagtct tggagtatca caagcatttt tatattgggg
     7381 tattatcgtc ttactattag tgttgatagg atcattcttc ttacgtgaag caattgtaag
     7441 taacactgta actgaaacat tacacaatga ttatacgccg cgtgaaatga tgaaaacaaa
     7501 acaagtatat ctcttatttt tcatgttatt cacatcatgt atgggcggac tgtatttaat
     7561 cggtatggta aaagacatcg gggtgcagct cgttggtctt agtgcagcga cggccgctaa
     7621 tgccgtcgca atgattgcaa tctttaatac aataggtaga attattcttg gaacgttatc
     7681 agataaaata ggtcgattaa aaatcgtctc ggctacattt attattattg gattatccgt
     7741 cttcacttta agttttattc cactaaatta cggaatttat tttgcttgtg tagcaagtgt
     7801 tgccttttgc tttggtggta acatcactat cttcccagca attgtgggag acttcttcgg
     7861 attaaaaaat catagtacaa actacgggat tgtttaccaa ggtttcggat ttggtgcact
     7921 tgcaggatca ttcattggag cactacttgg cggatttcag ccaaccttca ttacaatcgg
     7981 tgttctatgt gttatatctt tcgttatctc tatagtaatc cgccctccaa atgcagaaaa
     8041 gaagaaggaa atcaaatcgt taaatcgaaa aatagcttaa tgctaagaaa gagtccttat
     8101 actagggctc ttttgcttta caaaaaaatg tataaacaat gaatgacatt caaaaaatct
     8161 aattttttcc ctgtacttat ttcataatct aacattaaag aagataggaa attccgatta
     8221 ttaaattgaa taaataatgt aaaggggcgt gatagttaaa atgcaaatag taaagcaatg
     8281 ggtacaagaa gatagtgatt acataagaga aaaagtaatt gaatataacc aaaaacaact
     8341 ttcaaatgaa gaaaaaacac cttcagaaaa gattagtttt atagtgaaga atgaaaaaga
     8401 agaaatagta ggagggataa cggcaatcac attttggcac catgtatatg ttgacttcct
     8461 atgggtttct gaagaataca ggcatgaagg ttatggaagc aatttaatga atcttattga
     8521 agaatttgca ataaaaaaag aatgtaggct aatcaaatta gatactttta gtttccaagc
     8581 ccctaatttt tataagaagc atggatataa agtgataggg gtaagtgaag attatcctaa
     8641 agggcataat cattactatt tagaaaaaag gttataaatg aatcattaaa gagtgtatta
     8701 tgattaatct ttttcaaaat atgctctttt cttttgtgtg tctatgaaca cgatatcgtt
     8761 tcgtattagt tatttactca aattactttt tatgaatcaa aacaatttaa tgatagaata
     8821 aaataaagac agaaaattct caccattcaa gggggttaca tatgaaaaaa gtaaatgtta
     8881 catacgcact tctatatgat gaaactcatg aaaagctatt aatggttaaa aacaaagggg
     8941 aaaatggttc ttattacact ttaccaggtg gagcagttaa attcggggaa acgttagaag
     9001 aggcagcaat tcgtgaagtg aaagaagaaa cagggctaga catatccgta aaaggggttt
     9061 gttccattag tgaagccttt tttaaggaga gaggacatca tgcaatcttc tttaattttt
     9121 taggagaaat aaccggaggg gaaatctgta tatcacggcc aaaagagatt gaggagatta
     9181 cctggatgga attagattca gcagaacctt atttacgtat accagaacac ttaaaaagcc
     9241 ttttacaaaa gaaagaaaca gttccttata tttttaatgg aactattatt catcaatctt
     9301 cataaagata gtatttcatt taaggcgggg gaaaaatgga gtttatcgtt aatcacaaac
     9361 aatttacaca agctctttca gaagtaagta aagctatctc aacaaaaact ttaattccta
     9421 tattatccgg aataaagata acagcagatc aatctggaat tactttaatc gcaagtaatt
     9481 cgaatatttt cattgaaaaa tttataccta tttcaattga agacgaacaa attacaacta
     9541 ttttaaaagc aggaagtatt gttgtaccag caaagtattt cattgaaatt attaagaaaa
     9601 tgccaagtga gattttaata aaaagtatga atgagcagtt gattacaatt caatcagatg
     9661 aaattacttt aaacttaaat ggactctcgg caaatgaatt tcctaatgta ccatttttag
     9721 actcacatac agaaatacaa gtagaaacgg aacagctgat tgaggtattc aaacaaactg
     9781 tctttgcaac tgctaaaaat gaatctagac cagttcttac tggcgtacat ttcgttattg
     9841 atcataacaa attaatttgt gctgctactg attcgcacag actggcttta cgtgaaatac
     9901 ccatttcttc acatgcgaaa ttaaattgca ttgtaccaag ttccactatt agtgaactac
     9961 taaaattaat gagcagtaat tcaaatttag tatacatata tctttcagag agtcacatta
    10021 tttttaaatt cggtacaatt acgttgtatt caaggcttat tgaagggaaa tatcctaata
    10081 tatccggcct aattccaaat gaatcccaaa cgattattaa tatagataga aaaaagattt
    10141 tacaaggtgt agatcggtca agtttattgg ctagtgaatg ggcaaacaac aacgttaact
    10201 tagaaatcat cgacgaatgc acaatacaaa tttcttccaa cgcttctcaa attggtaaaa
    10261 tatccgagac acaaca
//
