LOCUS       NC_016109              10894 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Kitasatospora setae KM-6054, complete sequence.
ACCESSION   NC_016109
VERSION     NC_016109.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora setae KM-6054
  ORGANISM  Kitasatospora setae KM-6054
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1
  AUTHORS   Ichikawa,N., Oguchi,A., Ikeda,H., Ishikawa,J., Kitani,S.,
            Watanabe,Y., Nakamura,S., Katano,Y., Kishi,E., Sasagawa,M.,
            Ankai,A., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Nihira,T., Horinouchi,S., Ohnishi,Y., Hayakawa,M.,
            Kuzuyama,T., Arisawa,A., Nomoto,F., Miura,H., Takahashi,Y. and
            Fujita,N.
  TITLE     Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary
            snapshot of the family Streptomycetaceae
  JOURNAL   DNA Res 17 (6), 393-406 (2010)
   PUBMED   21059706
  REMARK    DOI:10.1093/dnares/dsq026
REFERENCE   2  (bases 1 to 8783278)
  AUTHORS   Ichikawa,N., Watanabe,Y., Nakamura,S., Katano,Y., Oguchi,A.,
            Ankai,A., Yashiro,I., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Ikeda,H., Ishikawa,J., Horinouchi,S., Ohnishi,Y.,
            Hayakawa,M., Nihira,T., Kitani,S., Kuzuyama,T., Arisawa,A.,
            Nomoto,F., Miura,H., Takahashi,Y. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2009) Contact:Director-General Department of
            Biotechnology National Institute of Technology and Evaluation, NITE
            Genome Analysis Center (NGAC), Department of Biotechnology; 2-49-10
            Nishihara, Shibuya-ku, Tokyo 151-0066, Japan URL
            :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000269985.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:32:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,713
            CDSs (total)                      :: 7,605
            Genes (coding)                    :: 7,487
            CDSs (with protein)               :: 7,487
            Genes (RNA)                       :: 108
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 25 of 118
            Pseudo Genes (incomplete)         :: 95 of 118
            Pseudo Genes (internal stop)      :: 9 of 118
            Pseudo Genes (multiple problems)  :: 11 of 118
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:33:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8651212
            Orig. end                         :: 8662106
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010968.1.
            'Otoguro, M. is at NITE Biological Resource Center (NBRC). Ikeda,
            H., Miura, H. and Takahashi Y. are at Kitasato Institute for Life
            Science. Ishikawa, J. is at National Institute of Infectious
            Diseases. Horinouchi, S., Ohnishi, Y. and Kuzuyama, T. are at The
            University of Tokyo. Hayakawa, M. is at University of Yamanashi.
            Nihira, T. and Kitani, S. are at Osaka University. Arisawa A. is at
            Mercian Corp. Nomoto F. is at Nagase & Co. Ltd. The other authors
            are at NITE Genome Analysis Center (NGAC).
            Please visit our web site. URL:http://www.bio.nite.go.jp/
            DOGAN ; Database of Genomes Analyzed at NITE. The database contains
            genome sequence data, physical maps and the genomic and proteomic
            data of the microorganisms analyzed.
            URL:http://www.bio.nite.go.jp/dogan/Top
            The microbial strain and microbial genomic DNA clones used for the
            sequencing project are available through the NBRC.
            URL:http://www.nbrc.nite.go.jp/e/index.html.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10894
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="47"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10894
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10894
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5894](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5894)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    901..910
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(1915..2832)
                     /locus_tag="KSE_RS37400"
                     /old_locus_tag="KSE_75630"
     CDS             complement(1915..2832)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013232.5"
                     /locus_tag="KSE_RS37400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_75630"
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="WP_051055596.1"
                     /transl_table=11
                     /translation="MVVLTALGLEYEAVRAHLSGLRRVVHGGTVFETGVLGTTGRAVAL
                     AEVGPGNRPTAVVTERARQHLDPWAVLFVGVAGALKRDVALGDVVVASRIHAYESAKHT
                     PAGTLPRPQSWPASHPMLQAARHALRGREWHAGIPSDARGRWEGAGPAVHFKPIAAGEA
                     VLNTSQGEVRRLLAEAFEDAAAIEMEGAGLAEAASIGGLEALVLRGLSDYADGAKATAD
                     ASGSQPLAAAHAAAAAAAVIAALPAGPGEPPDSRGTGKGPGAGEPGAVGGPVQVNTAYT
                     QGTVNAVQKGSLTIRGDRRLPPGV"
     misc_feature    2838..2851
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.74"
                     /tool="antismash"
     misc_feature    complement(2838..2853)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.15"
                     /tool="antismash"
     gene            3002..3529
                     /locus_tag="KSE_RS37405"
                     /old_locus_tag="KSE_75640"
     CDS             3002..3529
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSE_RS37405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSE_75640"
                     /product="hypothetical protein"
                     /protein_id="WP_014140608.1"
                     /transl_table=11
                     /translation="MEEEATSIKALFLEYQQTKEEQKSRIAFRDNLPYVTLGLVAAVLV
                     AGSLSTPLLLLISAATPALGWLYLANDKKISDMGRYIRTSLDPRLGAISQEQVTMFAWE
                     HFHRSDAGRTQRRTIQCAVDLALFTGIPTVALAVCWSGGRNSALSLAVSAIEVAAVITL
                     AIHIVKHAKSSH"
     gene            complement(3589..4287)
                     /locus_tag="KSE_RS45470"
                     /old_locus_tag="KSE_75650"
     CDS             complement(3589..4287)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF025063.5"
                     /locus_tag="KSE_RS45470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_75650"
                     /product="integrase core domain-containing protein"
                     /protein_id="WP_014140609.1"
                     /transl_table=11
                     /translation="MLRLLRDEGLLLEANYQRERRQLAARRKAAFATEPTGPNQVWQLD
                     FSEFETAGSGIWRLAGCRDYWSKYELDWHISPTANQHDAIAAIELAVKEAESLAGKPLA
                     EFATRDADGTVKPLVTIVTDNGGPFRSFRIEAFMATRPELRHIRTRVRTPGQHGSRERG
                     FGSLKYEKPFLEEIPDALDLVRHAEDYRRDYNTLRPHEALAWNQPHDVHTGAADPLVPN
                     FPEHENLPIA"
     gene            complement(4330..4515)
                     /locus_tag="KSE_RS43930"
                     /old_locus_tag="KSE_75660"
     CDS             complement(4330..4515)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSE_RS43930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSE_75660"
                     /product="hypothetical protein"
                     /protein_id="WP_014140610.1"
                     /transl_table=11
                     /translation="MPTARFCRLIGVPERTWRRHQARARQGAPTRGPWPRPARESVRET
                     ARRHALAHPAWGHRKV"
     gene            complement(4488..4847)
                     /locus_tag="KSE_RS37415"
                     /old_locus_tag="KSE_75670"
     CDS             complement(4488..4847)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024908.5"
                     /locus_tag="KSE_RS37415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_75670"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_041293924.1"
                     /transl_table=11
                     /translation="MVRPPALPPEEKVRIVLSILAGETTVAEAARRAKVSEQSIGTRKR
                     QFLESGRAGLAGKSGPSTREQQLEAQVADLTQAPGEAPVEPRVWKKSAEGRLGPSRTSR
                     RPAPRRACRPRGSAG"
     gene            complement(5001..5894)
                     /locus_tag="KSE_RS37420"
                     /old_locus_tag="KSE_75680t"
     CDS             complement(5001..5894)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133278.1"
                     /locus_tag="KSE_RS37420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_75680t"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_158413044.1"
                     /sec_met_domain="AfsA (E-value: 2.2e-21, bitscore: 74.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKEYVHRASLSEVFLTGWHQTAPDTFTVTAQWPRSHSFYTTDHH
                     THDPLLLSETIRQTFPLLTHAAYHLPFGHQLSWSHFQIRLDPRAMRIEPTPAEIELHVT
                     ATNIRYHRGLPASMTMHYTARRDGQPLATATTHFHCHTPTVYQRLRAGRATTAHPFHTA
                     PPPPHPLTPTTAGRTHHHDIVLSPTPHPHHWQLRVDTHHPVLFDHPVDHVPGMLLLEAT
                     RQATNTLTTPRKPHIITHLDITFNRYVELDTPCYIHAHTTHHNPTNHHNPTHHITATQA
                     DTTAFTAHTHTTPLPT"
     misc_feature    complement(6075..6089)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.37"
                     /tool="antismash"
     misc_feature    complement(6076..6089)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.5"
                     /tool="antismash"
     gene            6134..6775
                     /locus_tag="KSE_RS37425"
                     /old_locus_tag="KSE_75690t"
     CDS             6134..6775
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133277.1"
                     /locus_tag="KSE_RS37425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_75690t"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_014133277.1"
                     /transl_table=11
                     /translation="MAEPKQERAVRTREVILYAAAEVFDECGYSGASISKIMQRSGATQ
                     GGMYFHFKSKEGLAHAVMASQQEFIRLPPGRDGLQRLIDITFHIAGELQRNVLFRASVR
                     LAVEQGELGLEDDTAYQEWVQRFHAQLVAARECGELLEGVDEWEFATVLVGAYTGTQIF
                     SNVATGRADLPERIASLWRYLLPAVATPQAAVLLRVEPSVGPPSGRVSAG"
     gene            7855..8811
                     /locus_tag="KSE_RS37430"
                     /old_locus_tag="KSE_75700t"
     CDS             7855..8811
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.5"
                     /locus_tag="KSE_RS37430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_75700t"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_269450280.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.4e-05, bitscore:
                     21.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSGATGFVGSVVLEGLVRGWAGAAGGGGVELRVVSRRPPVGGGG
                     VVEWVWGDLAEPASLRGVCEGADVLVHLASYIGADEGMCEAVNVAGSAALVGEAVRAGV
                     GRIVGLSTAAVYGVGPHSGIGVDEVEPVPVSVVSRTRLAGEGPVLAAGGIVLRPGLVLG
                     EGDRWVVPALAELVERVPARWEGGRGLLSAVAVGDLGRLIVRLACASRAVGGGLYHASH
                     PVPVRVGDLLAVLAAHGVLPSVAQREELSWEACLERLRARPGRVSARQLELLTRDHWYR
                     SERVWRAAGCPAGPGVLARTAQAARWYRSHLDPRPTA"
     gene            complement(9637..10836)
                     /locus_tag="KSE_RS37435"
                     /old_locus_tag="KSE_75710t"
     CDS             complement(9637..10836)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133275.1"
                     /locus_tag="KSE_RS37435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_75710t"
                     /product="transposase"
                     /protein_id="WP_014133275.1"
                     /transl_table=11
                     /translation="MAEDMSRAAAALAQSRLSAFTEELFGHLPRADQRRWAQAYLRGLL
                     VTPGKKSVRRLASAVSDSPTASQSLQQFINASPWDWRPARRELTRVVEQHAPTEAWSVA
                     AAVLPKRGDHSCGVHRRFVPSAGRTVNCQVGVGVFLSSGGQDVPVDWRLLLPDQWDDDA
                     VLRERTRIPPAVRHRPLWAHTLDLVDTLASRTSQAPAPVVADMSEYPDAGRLVEGLSRR
                     GRDFVIAVPGTLRVVPGGLRAAAPDLHAPLSARQLLYPSGTRHPHTATLPAPDRRLRSV
                     RILSGLARLHLPRQDAPAARHTYRLFTQWRPGGGQQPARMWITNLVDRRIDRLIALSQL
                     HRGTTTAMRSLEQEYGMLDFEGRSYPGWHHHMTLVSAAYTYNRLVHPAQQPGEPVHQSA
                     "
ORIGIN
        1 tccccgcgtc gtgcgggtcc gtgcagtagt acgccgccgc gagccgcagc agcaggtccg
       61 gatcacgggc cgccagagcg cgtgcgggat cgaagcggtc caccaccgga gccagttcgc
      121 cccggtcggc caaggccacc gcccgcagcg cccgcgccac gtgttcgtcc aggtcggcgc
      181 cgagcagccc caaggcctcc aaccgcgcct ggcccctgct tccacggccc ccgccgctca
      241 gcagacgctc gcgcaccaga acccgcgcgg cacggtaggg cgacacgtcg gatcccgccg
      301 tctccaaccg cgcgaccccc cgcagccagc cgagcaccac ctcctcgccc cgggacggct
      361 catggtcggc accggaggcg aacagggcag tgttcgcagc cagccacgcc acgaccggcg
      421 ctgcgaccac cacatcgcac gccccctcgc cggcgaaccg cagctgcacg catgacagca
      481 gactgtccag caatagcggg tcgccgccca gcgaagcgga cagcgcttcc agcgcctgct
      541 cgcaccaacc cgccgccagc accgcctccc acggcacctc cgcccaccgc ggcccgtgga
      601 ctccggccag cgccgcgaac ctggccgaca cctcctccca gcacgcaagg aaggcaccgg
      661 tcgcgctgcc gcgctgccgc agcctcacct ggcacacgat ccgcgccgcc cgcaccgccc
      721 accggtgccc cgacgcctcc agcagcggca ggccgtcctc cagcaggagc cgccgggcca
      781 cggcgtagtc ccgcagcacg tcatgcgcga actgctgctc ctcgcccccc agcgccgacc
      841 cctcgcccac cggcgccagg atccccgccg aacgcagcga cgccagcgtc cggccggtgg
      901 gaaggcggcc ccggcgtgcc gtcagccgcc cttccgccaa cgacaccagc gcgtcggccc
      961 ggtcatcccc cgacccctcg tcatcggcac cagggcgccc gccctgcagg atcagtgcct
     1021 gccacaccac ggagtagacg tcggcctcgc tggacagcac ctcgggcaga cgggcgccgg
     1081 tgcgtgtggc gcgcagcagc aggtcgacgg tggtcagccg gcccagcaac cagcgcgacc
     1141 tggcgtcgcg ggcgatccgg gcgagttcgg gggcggcggc cagcacctgg gcactctcgc
     1201 cctcgtccaa cgagcggaga gtgaactccc gaaccgggcg ccctgccagc tccagaatct
     1261 gccgcagggt gtccaccgca tcgtcgcggc tgaccaggac gagagtcgcg cccaggcccc
     1321 aggccgcttc ggcggcctcg gccaccaggt cctccaagcc ctgctgcgcc gcctccgcgc
     1381 cgtccaggag cacggcacgg cccggtggcc tctgctcccc gccatcggcg tgcccgtccg
     1441 cgccgatccc tgcggccagc gccctctcca gcaggtcctc cagcccgtcc cggtggccgg
     1501 ccagggcggg caagggcgcg gcgagcacgg tgaactggcg ccgcctcagc tcctcggcca
     1561 cacccagtgc cagggccgac ttcccggtgc ccggctcccc gcgcaccagg acgaccgcct
     1621 gtgctcccgc atcggtgaca cagcccgaaa ggtcgcgcac ggccgcgccg cggtcgatcc
     1681 gcaactcctc accgccgccg gtgcggatga agccgggcgt gagcctgcgc agcctgcgct
     1741 cccactgggc gaacaggacc ggggcatcca cagtggcgcc gtagtggatg acgtcgccgt
     1801 gctgggaggc gtagacgctc ccgtgggcgg aggcggtgtt gacctgcacc ccatcctgct
     1861 gtgattcctg acgggggagt gccccgcccg cgtcctcagg cggccgggtc atcctcacac
     1921 ccccggcggc agccgacggt ccccacggat cgtcagggac cctttctgga ccgcgttgac
     1981 cgtgccctgg gtgtacgcgg tgttcacctg gaccggaccg ccgaccgcgc ccggctcgcc
     2041 cgccccgggc cccttgccgg tgccgcggga atccggtggc tcacccggcc ccgcagggag
     2101 ggcggcgatg acggcggcgg ccgcagcggc cgcgtgggcg gcggccaggg gctgcgaccc
     2161 ggatgcgtcc gccgtggcct tcgccccgtc cgcgtagtcg ctcaagcccc ggagcacgag
     2221 cgcctccaaa ccgccgatgc tggcggcctc ggcaaggccg gccccctcca tttcgatcgc
     2281 cgccgcgtcc tcgaaggcct ctgccagcag cctgcgcacc tcgccctgcg acgtgttgag
     2341 gacagcctcg ccagccgcga tcggcttgaa gtgcactgcc ggccccgccc cctcccaccg
     2401 gccgcgcgca tcgctgggaa tccctgcgtg ccactcccgg ccgcgcaggg cgtgccgggc
     2461 cgcctgcagc atcgggtggg aggcgggcca ggattgcggg cgggggagtg tgccagccgg
     2521 cgtgtgcttg gcgctctcgt aggcgtggat ccggctcgcc accaccacgt cccccagcgc
     2581 cacgtcccgc ttcagggccc ccgcgacgcc gacgaacagc actgcccacg ggtccaagtg
     2641 ctgcctggcc cgctcggtca ccacggcggt cggtcggtta cccgggccga cctcggccaa
     2701 ggcaaccgcg cgccccgtcg tgccgagcac ccccgtctcg aagacggtcc cgccatggac
     2761 aacgcgcctc aggccggaca gatgggcccg aaccgcctcg tactccagcc ccagtgcggt
     2821 cagcaccacc acaggccgag aatcactacc actcatcaga cccccaaaga aagacaacac
     2881 tcctgcgaga acggactttc cctcgttcga aggtaccggc cccatgccac ggcgggccgc
     2941 atcgcatccc ctcgatccac tggcaacaat agctccaccc tccgacagtg aaggagccct
     3001 agtggaagaa gaagcgacct cgatcaaggc cctcttcctc gaataccagc agaccaaaga
     3061 ggaacagaaa tccaggatcg ccttccgcga caacctcccc tacgtcaccc tcggcctcgt
     3121 cgccgcagtc ctcgtcgccg gctccctctc cacgcctctg ctcctcctga tctccgccgc
     3181 gacccccgct ctggggtggc tctacctggc caacgacaag aagatcagcg acatggggcg
     3241 ctacatccgc accagcctcg acccccggct cggcgccatc agccaggagc aggtcacgat
     3301 gttcgcctgg gagcacttcc accgcagcga cgccgggcgg acccagcgcc ggaccatcca
     3361 gtgcgccgtc gacctcgcgc tcttcaccgg aatccccacc gtggcactgg cagtttgctg
     3421 gtccgggggc cggaactccg ctctcagcct ggcagtttcc gccatcgaag tagcagctgt
     3481 gatcacattg gcaatccata tcgtcaagca cgccaaatcc agccactgat cgaagccgta
     3541 cctgcagtga ggcggtcacg atccgccaaa atacgaatct ttcccgcgtc aagcaattgg
     3601 caggttttcg tgttcgggaa agttgggcac gagggggtcc gcggcgccgg tgtggacgtc
     3661 gtggggttgg ttccaggcca gggcctcgtg cggtcggagg gtgttgtagt cacgccggta
     3721 gtcctcggcg tgccggacga ggtcgagggc gtcggggatc tcctcgagga acggcttctc
     3781 gtacttgagc gagccgaagc cgcgctcgcg ggagccgtgc tggccggggg tgcggacccg
     3841 ggtgcggatg tgccgcaact ccggtcgggt ggccatgaac gcctcgatgc ggaacgagcg
     3901 gaatggtccg ccgttgtcgg tgacgatggt gaccagcggc ttcacggtcc cgtccgcgtc
     3961 gcgcgtggcg aactcggcca gtggctttcc ggccagcgac tccgcttcct tgacggcgag
     4021 ttcgatcgcg gcgatcgcgt cgtgctggtt cgcggtgggc gagatgtgcc agtccagctc
     4081 gtacttcgac cagtaatcgc gacagccggc cagcctccag atcccgctgc cggccgtctc
     4141 gaactcggag aagtcgagct gccagacctg gttcgggccg gtcggctccg tggcgaaggc
     4201 ggccttgcgg cgggcggcga gctggcgccg ttcgcgctgg tagttcgcct ccagcagcag
     4261 gccctcgtcc cgcagcagtc gcaacacggt cgcttgtgag acgcggtggc cgtcccagcg
     4321 gcacgtcgct tacaccttgc ggtggcccca ggccgggtgc gcgagcgcgt gccggcgggc
     4381 ggtctcccgg acgctctcgc gggccggacg cggccatggt cctctggtgg gcgcgccctg
     4441 gcgggcgcgg gcctggtggc ggcgccaggt ccgctcgggc acgccgatca gccggcagaa
     4501 cctcgcggtc ggcatgcccg cctcggcgcg ggtcgcctcg aggtcctcga agggcccagt
     4561 cggccctccg cggacttctt ccacacccgc ggctcgaccg gggcctcacc cggtgcctgg
     4621 gtcaagtcgg cgacttgggc ttcgagttgc tgttcccggg tgctggggcc ggacttgccc
     4681 gccagccccg cgcggccgga ctccaggaac tggcgtttcc gggttccgat cgattgctcg
     4741 gacaccttcg cccgtcgcgc ggcctcggcg acggtcgtct ccccggccag gatcgacagt
     4801 acgatccgga ccttctcctc cggcggcagt gccggcggtc tcaccatgat cgatcggact
     4861 ccttcacgca gcaggacccg cgacaaacga gccctgccac aaagtctgac gcgcgacagg
     4921 ggtgtgggga acggccgcct tggttgtttg ggggtggttt tcgggggtgg gtgggtgggg
     4981 tggttttcgg gggttggttg tcaggtgggg aggggggtgg tgtgggtgtg ggcggtgaag
     5041 gcggtggtgt cggcctgggt ggcggtgatg tggtgggtgg ggttgtggtg gttggtgggg
     5101 ttgtggtggg tggtgtgggc gtggatgtag cagggggtgt cgagttcgac gtagcggttg
     5161 aaggtgatgt cgaggtgggt gatgatgtgg ggttttcggg gggtggtgag ggtgttggtg
     5221 gcttggcggg tggcttcgag gaggagcatg ccggggacgt ggtcgacggg gtggtcgaag
     5281 aggacggggt ggtgggtgtc gacgcgtagt tgccagtggt gggggtgggg ggtgggggag
     5341 aggacgatgt cgtggtggtg ggtgcgtccg gcggtggtgg gggtgagggg gtgggggggt
     5401 gggggggcgg tgtggaacgg gtgggcggtg gtggcgcggc cggcgcgtag gcgttggtag
     5461 acggtggggg tgtggcagtg gaagtgggtg gtggcggtgg cgaggggttg gccgtcgcgt
     5521 cgggcggtgt agtgcatggt catggaggcg ggcaggccgc ggtggtacct gatgttggtg
     5581 gcggtgacgt ggagttcgat ctcggcgggt gtgggttcga tcctcatggc gcgggggtcg
     5641 agccgtatct ggaagtgcga ccagctgagt tggtggccga aggggaggtg gtaggcggcg
     5701 tgggtgagga gggggaaggt ctggcggatg gtttcggaca gcaggagggg gtcgtgggtg
     5761 tggtggtcgg tggtgtagaa gctgtggctg cggggccatt gggcggtgac ggtgaaggtg
     5821 tcgggagcgg tttggtgcca gccggtgagg aagacttcgg acagtgacgc gcggtggacg
     5881 tactctttgg ccactgtggt ggtgagtgcg ggccgtgggc cggtgtgagg ttgcgtggtg
     5941 acgagcatgc cgttcccctt ttttggcgtg cgaggtgggg ccggtggcct gtgggccgcc
     6001 cggtcggacg tgctgtgctc ggggtgtgtc actcccgtgg cgtacatggt gcaacaccct
     6061 gaccctagac cgaggttcgt ctgtaaaata gagggcgttc gttttgtttg ccgagtggaa
     6121 gcggggcctg gctgtggctg aaccgaagca ggagcgtgcc gtgcggacgc gtgaggtgat
     6181 cctttacgcg gcggccgagg tcttcgacga gtgcggttac agcggtgcca gcatcagtaa
     6241 gatcatgcag cggtcggggg cgacgcaggg cgggatgtac tttcatttca agtcgaagga
     6301 ggggttggcg cacgcggtca tggcgagtca gcaggagttc atccggctgc cgccggggcg
     6361 ggacggtctg cagcggctga tcgacatcac gttccatatc gcgggggagc tgcagcgcaa
     6421 tgtgctgttc cgcgcgagtg tgcgtctggc ggtggagcag ggggagctgg ggctcgagga
     6481 cgacacggcg taccaggagt gggtccagcg gttccacgcg cagcttgtcg cggcgcggga
     6541 gtgcggtgag ctgctggagg gcgtcgacga gtgggagttc gcgacggtgc tggtgggggc
     6601 ttacacgggg acgcagatct tctccaacgt ggcgaccggg cgggcggacc tgcccgagcg
     6661 gatcgcttcg ctgtggcggt atctgcttcc ggcggtcgcc accccccagg cggcggtgct
     6721 gttgcgggtg gagccgtcgg tcgggccgcc gtctggtcgg gtctcggcgg ggtgagcggg
     6781 ggccgggtgg agccgtcggt cgggccgccg tctggtcggg tctcggcggg gtgagcgggg
     6841 tgagcggggt cgggtggccg gtggtcgggg gcgctgtccg tttcggctcg gggtggtgcg
     6901 ccgtctggtc gggtctcggc ggggtgagcg gggtcgggtg gccggtggcc gggggcgctg
     6961 tccgtttcgg ttcggggtgg tgcgccggcg gggccggccg gcggggtcgc cgttggtccc
     7021 gtctccgtct ccgtctgcgg ggggtgctgt ccggttctcg cggctctggt cgtcggggcc
     7081 gggtcctggc tgcggggggt ggtgcgctgt tggtccgggg ccggcgctgt tcggtccggg
     7141 tgctgtccgc tgtccgctgt gcgggtcgtg gctggtcccg gggtgctgtc ggccggtggg
     7201 gtcggtgttc gggcgttgcc tggttccgtg gtcgggggtg gcgggggccg tcgtccggtg
     7261 tccggtgtcg ggtggcggcg gtgttgcggg tggtgcccgc cggccgggct cggcgggtgg
     7321 ttgcggggtg tctgtccggt ggtggcgggt gggccgggcg ggtccgcggg gtgcctttgt
     7381 gtccttgcgc cgtgcgtccg tcgcggcttc ccgcggcggg ggtggcgggg tggttcggtg
     7441 gccggccccg gccccggccc cggccccggg ctcggccccg cacccgcacc cggccccggg
     7501 tgcggccccg cacccgcacc cggccccggg tgcggccccg cacccgcacc cggccccggg
     7561 tgcggccccg ggtgtgggtt cgggtggtgg ggtggtgcgg attgccgggt gggctctggt
     7621 ggtgggtggt cgcggccggt tgaatggagg gtgttcgttt tgttcggggg gaggtcggcg
     7681 cgagtgtggt gggggagcgc gggtgggggt ggtgtggcgt ggtctgccgg gggtgccgtg
     7741 tcgccggggc cggggccggg gccggggccg gggccggggc cggggccggg gccggggttg
     7801 gggccgggtg ggtggcgttc gttgcgtggt cggcgggtgg ggggtcgcag gatcgtgctg
     7861 agcggggcga cggggttcgt cggttcggtg gtcctggagg ggttggtgcg cgggtgggcg
     7921 ggtgccgcgg gtggtggggg ggtggagttg cgggtggtgt cgcgtcggcc tccggtgggg
     7981 ggtggcgggg tggtggagtg ggtgtggggg gatctggcgg agccggcgtc gttgcgcggg
     8041 gtgtgtgagg gggcggatgt gctggtgcat ctcgccagtt acatcggtgc ggacgagggg
     8101 atgtgcgagg cggtcaatgt cgcggggagc gcggcgttgg tgggggaggc cgttcgggcg
     8161 ggggtggggc ggatcgtggg tctctcgacg gcggcggtgt acggggtggg gccgcacagc
     8221 gggatcggtg tggacgaggt cgagccggtg cccgtttcgg tggtgagccg tacgcggttg
     8281 gcgggggagg ggccggtcct ggcggcggga gggatcgtgt tgcggccggg cctggtgctg
     8341 ggggaggggg accggtgggt cgttcccgcg ctggcggagc tggtcgagcg ggtgccggcc
     8401 cggtgggagg gcggccgcgg gttgttgtcg gcggtggcgg tcggtgatct gggccggctg
     8461 atcgtccgtc tggcgtgcgc gtcccgggcg gtgggcggtg gtctgtacca cgcgagccat
     8521 cccgtgccgg tcagggtggg ggacctgctg gcggtgctcg ccgcccacgg ggtgctgccg
     8581 tcggtcgcgc agcgggagga gctgtcctgg gaggcctgcc tggagcggct gcgggcgcgg
     8641 ccgggacggg tgagcgcccg gcagctggag ctgctcaccc gcgaccactg gtaccgcagc
     8701 gagcgggtgt ggcgggcggc gggctgcccg gccggccccg gtgtgctggc ccggacggca
     8761 caggcggcac gctggtaccg ctcgcacctc gacccgcgcc cgaccgcctg accgcctgac
     8821 cgcctgaccg cccccgcccc cgcccccgcg gccccggcct cgcaccgtcc tgccgtggcc
     8881 gtggccgtgg ccgtgcccgt gcgggccgga cgccgccccg gcggccgcca ccgccgcccc
     8941 gccgtcaagg tgtctcgacg ggggcggtgc ggatgtccgc cgtccccggt gcggtgggcc
     9001 cggtgggtgc ggtgggtgcg ggtgttttcg gtgcggtggg tgcgggatgc cggggtccgt
     9061 gcgggggtgg ggggttggtt cgcggggggt gtgcggggcc cgctgtttcg gtgcgggacg
     9121 cccggacgcc cgccgcccgg acgcccggac gcccgggcac ccgccgtccg gacgcggcgg
     9181 ctcgggggaa ggggtggcgg ggcggagggt gtcggccgtc cccggtgcgg tgggtgcggg
     9241 tgttttcggt gcggtgggtg cgggatgccg gggtccgtgc gggggtgggg ggttggttcg
     9301 cggggggtgt gcggggcccg ctgtttcggt gcgggacgcc cggacgcccg ccgcccgccg
     9361 cccggacgcc cgggcacccg ccgtccggac gcggcggctc ggggggaggg gtggcgggtg
     9421 tggggtgttc cggggttcgc gcggggtggc gggctggtgc gtgccggcgg tgcgggcgtc
     9481 tttgggtgtg gcggggcgcg cggtgtccgg ctcccgtgcg gggggctgtc gggggagccc
     9541 ccgcgcgggc ggggggccgc tttcggggtg tccccggggg cggggcgctc tgatgtccgg
     9601 gcgtccgggc gtccgggcgt tcaggcgttc tggtgttcag gcgctctggt gtaccggctc
     9661 gcccggttgc tgggccgggt ggacgaggcg gttgtaggtg taggcggcgg agaccagtgt
     9721 catgtggtgg tgccagccgg ggtaggagcg gccctcgaag tcgagcattc cgtactcctg
     9781 ctccagggag cgcatcgccg tcgtcgtgcc gcggtgcagc tgggagaggg cgatcagccg
     9841 gtcgatgcgc cggtccacga ggttggtgat ccacatccgg gcgggctgct gcccgccgcc
     9901 ggggcgccac tgggtgaaca gccggtaggt gtgccgcgcg gcgggggcgt cctggcgcgg
     9961 gaggtgcagg cgggccagcc ccgacaggat gcggacactg cgcagccgcc ggtcgggggc
    10021 ggggagcgtc gcggtgtgcg ggtggcgggt gccggagggg tacagcagct ggcgggcgct
    10081 gaggggggcg tggaggtcgg gggcggcggc gcgcagtccg ccgggcacca cccgcagggt
    10141 gcccggcacg gcgatgacga agtcgcgtcc gcgccggctg aggccctcga ccagccggcc
    10201 ggcgtccggg tactcgctca tgtccgccac gaccggcgcg ggcgcctgcg aggtgcggga
    10261 ggcgagggtg tccaccaggt cgagggtgtg ggcccacagg gggcggtggc ggacggcggg
    10321 cgggatgcgt gtgcgctcgc gcagcaccgc gtcgtcgtcc cactggtcgg gcagcagcag
    10381 ccgccagtcc acgggcacgt cctgcccgcc cgaggacagg aacaccccca cccccacctg
    10441 gcagttgacg gtacgcccgg ccgacggcac gaaacggcgg tggacgccgc acgagtggtc
    10501 cccgcgcttg ggcagcacgg ccgcggcgac ggaccaggcc tcggtcgggg cgtgctgctc
    10561 gaccacccgc gtcagctccc ggcgcgcggg gcgccagtcc cacgggctcg cgttgatgaa
    10621 ctgctgcagc gactgggagg ccgtcgggga gtcggagacc gccgacgcca gccggcgcac
    10681 cgacttcttg cccggcgtga ccagcagccc ccgcaggtac gcctgggccc agcggcgctg
    10741 gtcggcgcgc ggcagatggc cgaacagctc ctcggtgaag gcgctgaggc gcgactgggc
    10801 gagcgcggcc gccgcgcggg acatgtcttc ggccatcctc tgtttcctcc ccggctgggc
    10861 tcctcccaag ataatagaga acgttcgctt tgtt
//
