LOCUS       NC_016109              10939 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Kitasatospora setae KM-6054, complete sequence.
ACCESSION   NC_016109
VERSION     NC_016109.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora setae KM-6054
  ORGANISM  Kitasatospora setae KM-6054
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1
  AUTHORS   Ichikawa,N., Oguchi,A., Ikeda,H., Ishikawa,J., Kitani,S.,
            Watanabe,Y., Nakamura,S., Katano,Y., Kishi,E., Sasagawa,M.,
            Ankai,A., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Nihira,T., Horinouchi,S., Ohnishi,Y., Hayakawa,M.,
            Kuzuyama,T., Arisawa,A., Nomoto,F., Miura,H., Takahashi,Y. and
            Fujita,N.
  TITLE     Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary
            snapshot of the family Streptomycetaceae
  JOURNAL   DNA Res 17 (6), 393-406 (2010)
   PUBMED   21059706
  REMARK    DOI:10.1093/dnares/dsq026
REFERENCE   2  (bases 1 to 8783278)
  AUTHORS   Ichikawa,N., Watanabe,Y., Nakamura,S., Katano,Y., Oguchi,A.,
            Ankai,A., Yashiro,I., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Ikeda,H., Ishikawa,J., Horinouchi,S., Ohnishi,Y.,
            Hayakawa,M., Nihira,T., Kitani,S., Kuzuyama,T., Arisawa,A.,
            Nomoto,F., Miura,H., Takahashi,Y. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2009) Contact:Director-General Department of
            Biotechnology National Institute of Technology and Evaluation, NITE
            Genome Analysis Center (NGAC), Department of Biotechnology; 2-49-10
            Nishihara, Shibuya-ku, Tokyo 151-0066, Japan URL
            :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000269985.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:32:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,713
            CDSs (total)                      :: 7,605
            Genes (coding)                    :: 7,487
            CDSs (with protein)               :: 7,487
            Genes (RNA)                       :: 108
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 25 of 118
            Pseudo Genes (incomplete)         :: 95 of 118
            Pseudo Genes (internal stop)      :: 9 of 118
            Pseudo Genes (multiple problems)  :: 11 of 118
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:33:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7471511
            Orig. end                         :: 7482450
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010968.1.
            'Otoguro, M. is at NITE Biological Resource Center (NBRC). Ikeda,
            H., Miura, H. and Takahashi Y. are at Kitasato Institute for Life
            Science. Ishikawa, J. is at National Institute of Infectious
            Diseases. Horinouchi, S., Ohnishi, Y. and Kuzuyama, T. are at The
            University of Tokyo. Hayakawa, M. is at University of Yamanashi.
            Nihira, T. and Kitani, S. are at Osaka University. Arisawa A. is at
            Mercian Corp. Nomoto F. is at Nagase & Co. Ltd. The other authors
            are at NITE Genome Analysis Center (NGAC).
            Please visit our web site. URL:http://www.bio.nite.go.jp/
            DOGAN ; Database of Genomes Analyzed at NITE. The database contains
            genome sequence data, physical maps and the genomic and proteomic
            data of the microorganisms analyzed.
            URL:http://www.bio.nite.go.jp/dogan/Top
            The microbial strain and microbial genomic DNA clones used for the
            sequencing project are available through the NBRC.
            URL:http://www.nbrc.nite.go.jp/e/index.html.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10939
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="40"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10939
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10939
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5939](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5939
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(378..1355)
                     /locus_tag="KSE_RS32755"
                     /old_locus_tag="KSE_66250"
     CDS             complement(378..1355)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004836.1"
                     /locus_tag="KSE_RS32755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_66250"
                     /product="aldo/keto reductase"
                     /protein_id="WP_014139680.1"
                     /transl_table=11
                     /translation="MEQRLLGRTGLRVSRLALGTLTWGRETDEHEAAEQLKAFVDAGGS
                     LVDTADVYADGGAEYLLSRLTDGLVPRSELVIATKAGALPGSPRPDTSRGHLLDALDGS
                     LRRLNTDHVDLWQVHAHDPATPAEETLHALDLAVSSGRARYVGLCQYSGWQLAKAAARQ
                     HALPGATPLASVQLEYSLLQRGIEREALPAALDAGIGLLARSPLGRGVLTGKYRHGVPP
                     ESRGSSPHPAGPVQPYLGETSRRIVDALATAADGLASTPLKVALAWVRDRPGVSATLLG
                     ARTSAQLQSSLSVEALTLPGEIRAALDDVSAPVHRYPDQGWTEL"
     gene            1586..2632
                     /locus_tag="KSE_RS32760"
                     /old_locus_tag="KSE_66260"
     CDS             1586..2632
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002496.1"
                     /locus_tag="KSE_RS32760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_66260"
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="WP_014139681.1"
                     /transl_table=11
                     /translation="MRLGINLGYWGLGLDADNIAVAQEADRLGYAVCWAAEAYGSDAAT
                     VLSYVAAKTERIDVGSAIFQIPARTPAMTAMTAATLDTLSGGRFRLGLGVSGPQVSEGW
                     YGVRFDKPLARTREYVEIIRKAMSRERLVHEGANWTLPLPGGPGKPLKLTVHPVREHLP
                     LYIASMGPKNLELTGEIADGWLGLFFAPEHAALSTDALAAGRAKAGRTLDGFDLCPTVN
                     IAVGEDVRAAADTLRDYTALYLGGMGSKEKNFYSQLARRMGFEQAADQVQTHYLARDKA
                     AAAAAVPHEFIDATSLLGDTARIADRMQAYAAAGVTTLTLAPAGWTLDERIAGLRTGVE
                     ALERAGLA"
     gene            2823..3218
                     /locus_tag="KSE_RS32765"
                     /old_locus_tag="KSE_66270"
     CDS             2823..3218
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSE_RS32765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSE_66270"
                     /product="hypothetical protein"
                     /protein_id="WP_014139682.1"
                     /transl_table=11
                     /translation="MDPQLLNEIVPLLASVAGGAATEVGRGAAESSVARVRELVRRWRA
                     RGSAEPEQQAPPERQEQVRALLLAALDEDPALREELAGLLAPGPKNRTSVTSSSGVVIG
                     GDNHGPITLNGPGAGNFHQHLHRGDGG"
     gene            3272..3694
                     /locus_tag="KSE_RS43110"
                     /old_locus_tag="KSE_66280"
     CDS             3272..3694
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSE_RS43110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSE_66280"
                     /product="hypothetical protein"
                     /protein_id="WP_014139683.1"
                     /transl_table=11
                     /translation="MIGVRALPSQHPSRTPQGRADVRLGEGRWGGGGNRGVRPGDQRTF
                     GFDSTESRAYLVLTLGVLSERAGDAACRPGAELTVAATSGTIAPVVAKDGRPVVIALPA
                     TLPREFRLTLADYDNCLIDVFVNDAVLTDNDPSGTR"
     gene            3709..4725
                     /locus_tag="KSE_RS32775"
                     /old_locus_tag="KSE_66290"
     CDS             3709..4725
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012707.5"
                     /locus_tag="KSE_RS32775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_66290"
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="WP_014139684.1"
                     /transl_table=11
                     /translation="MPGAHRTLLAGALAGALLLLTGCTGDTPPPPLPSTPTVSASGPPS
                     IFLQKSVRIGTKNNQPGTSTQNKEHVWSGFDVLVGRSVVRNLGRSSADAAFSDVISGER
                     EAKLTTDAEDLVVATYSITPERTEKVFFAGPYAKTSQGFMVRADETWLGSLDALRGRTV
                     CSWGGTTSDTELRFQATVKGYTVASATDAQACLTELREKRVDAVSTDQLILYGLARNDP
                     GLKVVPEVKIGVPNFYGIGIKKERIEDCLRIRDYLKDYVLSSAWIQDFTAELPNAGPPD
                     EYLPKPTDFDEQSCRELPHQSPVSPSPSASPSASASASASPSPSPSADPEPTAAATP"
     gene            5001..5939
                     /locus_tag="KSE_RS32780"
                     /old_locus_tag="KSE_66300"
     CDS             5001..5939
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSE_RS32780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSE_66300"
                     /product="hypothetical protein"
                     /protein_id="WP_014139685.1"
                     /sec_met_domain="AfsA (E-value: 1.8e-07, bitscore: 29.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHTTPPSPLSPLPSRIPDVHGSRLISPLRRPEHGEGWLADLVVD
                     RRDPFFFDRPVDHVPGMLLVCAMADLVAAGDAVPAAGRVEAAIAFHTTAALGTPTELRA
                     DSFEDGRRRAVQVFQPATLIADGWAEFSPAATAPGRTASAPMPVSAPVPVPVPVPVRSA
                     HRGRPENVLIGDPSEPGGVVTAAVLSPPAGHPLGGRRPGTRRTEAIIESGRQLSAWLAH
                     RTTDWPPDARLLPHGLTIDLPYGLPDTLPLALRRRPAPISAHRTEYRYDLITRDGAGSR
                     PLGTLAYTARILRPRTRTGFRANRDVRRDAA"
     misc_feature    6101..6117
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.41"
                     /tool="antismash"
     gene            6109..7149
                     /locus_tag="KSE_RS32785"
                     /old_locus_tag="KSE_66310"
     CDS             6109..7149
                     /NRPS_PKS="Domain: PKS_ER (23-265). E-value: 1.4e-08.
                     Score: 26.1. Matches aSDomain:
                     nrpspksdomains_KSE_RS32785_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381456.1"
                     /locus_tag="KSE_RS32785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_66310"
                     /product="zinc-binding dehydrogenase"
                     /protein_id="WP_014139686.1"
                     /sec_met_domain="ADH_N (E-value: 2e-22, bitscore: 77.9,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 5.3e-25, bitscore:
                     86.7, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVVYERPGRVALRTVPDARIERPGDAVVRVAAAGVCGSDLIFV
                     GRGLYQAGQRCGHEFLGVVEETGRAVTALRVGDAVLAPFCWADGDCRHCLAGLLTSCAD
                     GGVWGLGEGSDGGQGEAVRVPHADATLVRLPAEALTDRRLAPAVLALADVLPTGHHAVV
                     AAGVRPGSTVAVVGDGAVGLCAVLAAARAGAERIIAVGSHPGRLRLALRFGATDTLAAR
                     GADADRRLAELTDGHGVDAAVEAVGTTPALETAIRATRPGGTVGFVGLPHHDTAPDLGP
                     AFFHNVALRGGIAPVRRYLPELLPEVCAGSLDAGALFDFTGTLADVPAAYDAMSGRTVL
                     KALIRP"
     aSDomain        6178..6903
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KSE_RS32785_PKS_ER.1"
                     /evalue="1.40E-08"
                     /label="KSE_RS32785_PKS_ER.1"
                     /locus_tag="KSE_RS32785"
                     /protein_end="265"
                     /protein_start="23"
                     /score="26.1"
                     /tool="antismash"
                     /translation="RPGDAVVRVAAAGVCGSDLIFVGRGLYQAGQRCGHEFLGVVEETG
                     RAVTALRVGDAVLAPFCWADGDCRHCLAGLLTSCADGGVWGLGEGSDGGQGEAVRVPHA
                     DATLVRLPAEALTDRRLAPAVLALADVLPTGHHAVVAAGVRPGSTVAVVGDGAVGLCAV
                     LAAARAGAERIIAVGSHPGRLRLALRFGATDTLAARGADADRRLAELTDGHGVDAAVEA
                     VGTTPALETAIRATRPGGTV"
     gene            7212..8810
                     /gene="mdlC"
                     /locus_tag="KSE_RS32790"
                     /old_locus_tag="KSE_66320"
     CDS             7212..8810
                     /EC_number="4.1.1.7"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mdlC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF005485.1"
                     /locus_tag="KSE_RS32790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_66320"
                     /product="benzoylformate decarboxylase"
                     /protein_id="WP_014139687.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.9e-36, bitscore:
                     122.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.2e-26, bitscore:
                     91.3, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAAARPPGTVRDAVVDVLRHHRAHVLFGNPGTTELPFLADLPPD
                     LRYVAGLNESSVTAIADGYAQATGRPVLVNLHTAPGLGNAMGSLVNAYAAGSPLVVLTG
                     QQRRDLIGADAILTNRNPVLLPLGAVKHAEEPAGAQDVPAALARAFHLAGLPPTGPVAV
                     SVPMDDWDRPLRPAPATTAITTRVTGGATAPADVLEELADRLAAARSPALVVGPGTDDR
                     RGYWAVAELADRLAAPVWVTPWAHRLGFPTDHPGFVGQLPLDSGPAAAALEPHDLVLVL
                     GGPVFRYMFAEQWPALAPGTELVALTDDPGQAARNLAGETVLCDVPGTVRALCRALADR
                     GSERPPGPPPARPPAEATPGYAQAALALHQVMPPEAVLVVEPPQSYEYLLRALPRTRPG
                     SLYFAAACGLGFALGAAVGIQLAEPERPVVALVGDGAVQYNLQALWTAVLERLPITVLV
                     VNNGGYGILKDFADRGRLSAVPALDLPHIDLTALARGYQVPALRVPADPARIAAALRTP
                     LPPGPRLIEVVVPAR"
     misc_feature    8898..8911
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.75"
                     /tool="antismash"
     misc_feature    complement(8900..8909)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.56"
                     /tool="antismash"
     misc_feature    8912..8929
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.51"
                     /tool="antismash"
     gene            9021..10292
                     /locus_tag="KSE_RS46035"
                     /old_locus_tag="KSE_66330"
     CDS             9021..10292
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015696.5"
                     /locus_tag="KSE_RS46035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_66330"
                     /product="ALF repeat-containing protein"
                     /protein_id="WP_014139688.1"
                     /transl_table=11
                     /translation="MSIRRSIAAAVFVAAVSAPTVFGTAQAMAETNPTGQSVPDAVLPD
                     TEPAPAPAPESPVAESPAPSPTAESPSPAAESPAPSPTAESPSPAAESPAPSPAAPAPA
                     EAAPAAPAAPAQPAAPAAPAQPSASDEAALLARLEALIAEPGHSRYFYEHAEEARGRDA
                     EGIRHFLDVEAPRIFLEDRRVEVAQAIYRAGQGSDIAKAGSKALDDGSPEAIEHFLNVT
                     LPALIVSDQRVQISRLIDEPGIGAKLREAALKALEDGRPAVVTAFMANLPQLRFADDLL
                     RASKAMSDGGPEVRKAAGAALDAGTPQALRAFITTGLAEARAKDKAAQEQQNQEQQNQN
                     QGQGQGQQGGTTVTPASDTRVATGSTGTTATTTTTATTGAGSTAAQLASTGTDAPVGSL
                     AFGGAAAIALGAGALVVTRRRRES"
     misc_feature    complement(10556..10571)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.13"
                     /tool="antismash"
     misc_feature    10661..10675
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 tccgaggcgg gctcttcgac cgccgcagcc ccttcagccg catcggacgg ctcggaccgc
       61 tcggacggct cgtcaggggt cgccaggtcg acctcccgtg gcgggcgggg cggggcgacg
      121 atgtcgttcg cgttgatcgc gccgatcgcc ttggcgagcg cggccaccgc ggcggcgcgc
      181 tgctcgccgg tgagcggcgg gtcagcgggc cgtccggcgc cgccgtccgg ggcggcggcc
      241 gggccctgcg gggcggcggg gccctccggg gtgcccgtcg gctccgccgg gcgctcggcg
      301 cggctctcgg cctggctctc ggccgggttc tcctcggggg tggtcatcgc gggaaggctc
      361 cttcgacggg cgggcggtca cagctccgtc cagccctggt ccgggtagcg gtgcaccggg
      421 gccgacacgt cgtccagtgc agcgcggatc tcacccggaa gcgtaagggc ctcgaccgac
      481 agcgatgact gcagttgggc ggaggtgcgg gcgcccagca gggtggccga cacgccgggg
      541 cggtcgcgga cccaggcgag cgcgaccttc agcggggtgg aggcgaggcc gtcggcggcg
      601 gtggcgagcg cgtccacgat ccgccgggag gtctcgccca ggtagggctg gaccggtccg
      661 gcggggtgcg gggaggagcc gcgcgactcg ggcggcacgc cgtgccggta cttgccggtc
      721 agcacgcccc ggccgagcgg tgagcgggcc agcaggccga tcccggcgtc cagcgcggcg
      781 ggcagtgcct cgcgttcgat gccgcgctgc agcagcgagt actccagctg gacggaggcc
      841 agcggggtgg cgccgggcag cgcgtgctgc cgggcggcgg ccttcgccag ctgccagccg
      901 ctgtactggc agagtccgac gtaccgggcc cggccggagg agaccgccag gtcgagggcg
      961 tgcagggtct cctcggcggg ggtggccggg tcgtgcgcgt gcacctgcca caggtcgacg
     1021 tggtcggtgt tcagccggcg cagcgagccg tccagcgcgt ccagcaggtg gccgcgggag
     1081 gtgtcggggc ggggcgagcc gggcagtgcg cccgccttgg tggcgatcac cagttcggat
     1141 cgcgggacca ggccgtccgt cagccgggac agcaggtact cggcgccgcc gtccgcgtac
     1201 acgtcggcgg tgtcgaccag ggagccgccg gcgtccacga acgccttcag ctgttcggcg
     1261 gcctcgtgct cgtcggtctc ccggccccag gtgagggtgc ccagggccag ccgggagacc
     1321 cgcaggccgg tccggccgag cagtcgctgt tccatcgcgc cctccgtcac gcccgctgtg
     1381 ccaaccgtga tggcgcaggt ggggggcggt ctccccccac gtgctgtgag ggtagcggcg
     1441 aaacccgccg gggcccggga tcacccgcgc gccgcgccgc cgcgcgcgtc ccggtgtgac
     1501 cggcaccacc cctaccgctc agtagcatga cctccggccg ggcggctacg ctcaccggca
     1561 gcacccgact ggaaggcttg ggcacatgcg actcgggatc aacctcggct actggggact
     1621 cggcctggac gccgacaaca tcgcggtcgc ccaggaggcc gaccgcctcg gctacgcggt
     1681 ctgctgggcc gccgaggcgt acggctcgga cgccgccacc gtcctctcct acgtcgccgc
     1741 gaagaccgag cggatcgacg tcggctccgc catcttccag atcccggccc gcaccccggc
     1801 gatgaccgcg atgaccgccg ccaccctgga caccctctcc ggcggccgct tccggctcgg
     1861 cctcggcgtc tccgggccgc aggtctccga gggctggtac ggcgtccgct tcgacaagcc
     1921 gctggcccgc acccgcgagt acgtggagat catccgcaag gcgatgtccc gcgagcgcct
     1981 ggtgcacgag ggcgccaact ggacgctccc gctgcccggc ggccccggca agccgctcaa
     2041 gctcaccgtc cacccggtcc gcgagcacct cccgctgtac atcgcctcga tgggcccgaa
     2101 gaacctcgaa ctcaccggcg agatcgccga cggctggctc ggcctcttct tcgcccccga
     2161 gcacgccgcc ctctccaccg acgccctcgc cgccggccgc gccaaggccg ggcgcaccct
     2221 cgacggcttc gacctctgcc ccaccgtcaa catcgccgtc ggcgaggacg tccgggccgc
     2281 cgccgacacc ctgcgcgact acaccgccct ctacctcggc ggcatgggca gcaaggagaa
     2341 gaacttctac agccaactcg cccgccggat gggcttcgag caggccgccg accaggtcca
     2401 gacccactac ctggcccgcg acaaggccgc cgccgcggcc gccgtcccgc acgagttcat
     2461 cgacgccacc tcgctcctcg gcgacaccgc ccggatcgcc gaccgcatgc aggcctacgc
     2521 cgccgccggc gtcaccaccc tgaccctcgc cccggccggc tggaccctcg acgagcgcat
     2581 cgccggcctg cgcaccggcg tcgaggcact ggaacgcgcc gggctggcgt agcggccggg
     2641 cccgtcgccg ggcccggcga cggacccgtt ggtgggcccc gctggtgcct cggtcgaggg
     2701 ccccgacggg cttccccccg gcggggtccg actgatcgcg gcggccgtga ttgcccgctg
     2761 gccgaaggtg gtaggtagtg gcttgaacac tcaagtgtga tgatcggtca ggggggaacc
     2821 cgatggatcc gcaactactg aacgagatcg tcccgctgct cgcctcggtc gccggcggcg
     2881 cggccaccga ggtcggccgg ggggccgccg agagctccgt cgcccgggtg cgcgagctgg
     2941 tgcgccgctg gcgggcgcgg gggagcgccg agccggagca gcaggccccg ccggagcggc
     3001 aggagcaggt gcgggcgctg ctgctggcgg cgctggacga ggacccggcg ctgcgggagg
     3061 agctggcggg actgctcgcg ccgggcccca agaaccggac gagcgtcacc agttccagcg
     3121 gtgtggtcat cggcggtgac aaccacgggc ccatcacgct gaacgggccg ggcgcgggaa
     3181 atttccacca gcacctccac cggggggacg gcggctagcc cgtcgggccg tcctgtccgt
     3241 cgcggtcctg ggggcgctcg gcctggtcgt cgtgatcggt gtccgggcgc tcccgtcgca
     3301 gcacccgtcc cggacgccgc agggccgcgc cgacgtccgg ctgggcgagg gcaggtgggg
     3361 cggcggcggc aacaggggcg tgcggcccgg cgaccagcgg accttcggct tcgacagcac
     3421 cgagtcgcgc gcgtacctgg tgctcaccct gggcgtgctc agcgagcggg ccggtgacgc
     3481 ggcctgccgg cccggcgcgg agctgaccgt cgccgcgacg tccgggacga tcgcgccggt
     3541 ggtcgccaag gacgggcggc cggtggtcat cgccctcccc gcgaccctgc cccgggagtt
     3601 ccggctgacc ctggccgact acgacaactg cctgatcgac gtcttcgtca acgacgcggt
     3661 cctcaccgac aacgacccca gcggcacccg ctgaacgaag gagcccccgt gccgggcgca
     3721 caccgaaccc tgctggccgg cgccctggcc ggggcgctcc tgctgctcac cggctgcacc
     3781 ggcgacaccc cgcccccgcc gctccccagc accccgacgg tctccgcgag cgggcccccg
     3841 tcgatcttcc tgcagaagtc ggtgcggatc ggcacgaaga acaaccagcc cggcaccagc
     3901 acgcagaaca aggagcacgt ctggtccggg ttcgacgtgc tggtcggccg gagcgtcgtc
     3961 cggaacctgg gcaggtccag cgccgacgcc gccttcagcg acgtgatctc cggcgagcgc
     4021 gaggcgaagc tcaccaccga cgcggaggac ctggtggtgg cgacctactc catcaccccc
     4081 gagcggaccg agaaggtgtt cttcgccggc ccgtacgcca agacctccca gggcttcatg
     4141 gtgcgcgccg acgagacctg gctgggcagc ctggacgccc tcaggggccg caccgtgtgc
     4201 agctggggcg gcaccacctc ggacaccgaa ctgcggttcc aggccacggt caagggctac
     4261 accgtggcct ccgcgaccga cgcgcaggcc tgcctcaccg agctcaggga gaagcgcgtc
     4321 gacgccgtct ccaccgacca gctgatcctc tacggcctgg cgaggaacga ccccggcctg
     4381 aaggtcgtgc ccgaggtgaa gatcggggtg cccaacttct acggcatcgg gatcaagaag
     4441 gagcggatcg aggactgcct ccggatccgc gactacctga aggactacgt gctcagcagc
     4501 gcctggatcc aggacttcac ggccgagctg ccgaacgcgg gtccccccga cgagtacctc
     4561 ccgaagccga ccgacttcga cgagcagtcg tgcagggaac tgccccacca gagcccggtg
     4621 tccccgtccc cgtccgcgag tccgtccgcc tccgcctccg cctccgcgag cccgagcccg
     4681 agcccgagcg cggacccgga gccgaccgcc gccgccaccc cgtagggccg agcgggcggc
     4741 tggccggatc ggtacggccg ggcacgcggg gtgcgctgac ggtgcgcccg ctctgcccct
     4801 aggagcagcc cttctgcccg cacgcgcaga ggggctcctg gtgcatacct cgtatgcggt
     4861 ggcgtggcgc acgatcggat cgtccgcccg tcccggcggg cccgccggtc gaatccgacg
     4921 cggcgtcagg agcacaccgc ggcagcggag tcggaccggc ccgccggccc ggaacgcgag
     4981 gaagtcgagg caccggaacc atgacgcaca ccaccccgcc gtcaccgctg tcaccgctgc
     5041 cgtcccggat cccggacgtg cacgggagcc ggctgatcag cccgctccgg cggccggagc
     5101 acggggaggg gtggctggcc gacctggtcg tcgaccggcg cgacccgttc ttcttcgacc
     5161 gcccggtcga ccacgtcccc ggcatgctgc tggtctgcgc gatggcggac ctggtggccg
     5221 cgggcgacgc ggtgcccgcc gcgggccggg tcgaggcggc gatcgcgttc cacacgacgg
     5281 ccgcactcgg gacgccgacg gaactgcgcg ccgactcgtt cgaggacggg cggcggcgcg
     5341 ccgtgcaggt cttccagccg gccacgctga tcgccgacgg ctgggccgag ttctccccgg
     5401 cggccaccgc cccgggccgg accgcctccg cgccgatgcc cgtgtccgcg cccgtgccgg
     5461 tgccggtgcc cgtgccggtc cggtcggcgc accggggacg tccggagaac gtgctgatcg
     5521 gcgacccgtc cgaaccgggc ggcgtggtca ccgccgccgt gctgtccccg cccgccggcc
     5581 acccgctcgg cggccggcgc cccggcacgc gccgcaccga ggcgatcatc gagtccggcc
     5641 ggcaactctc cgcctggctg gcccaccgga ccaccgactg gccgccggac gcccggctgc
     5701 tgccccacgg cctgacgatc gacctgccgt acgggctgcc cgacaccctc ccgctggcac
     5761 tgcgccgacg gccggcgccg atctccgcgc accgcaccga gtaccgctac gacctgatca
     5821 cccgcgacgg cgccggaagc cgaccgctcg gcaccctggc ctacaccgcc aggatcctcc
     5881 gcccccggac gcgcaccggc ttccgggcca accgcgacgt ccgccgagac gccgcctgaa
     5941 ccaccacccc gccaccccgc caccgggcgc ccgcgcccgg ttcggacggg ttccgttttc
     6001 agccggtcgg gagtttcaac tgatcaccgg cagaaggcac ttaacgccgc tccggcggag
     6061 cccgaagctg gagtccggag cacggcggac ggaagcgggg tgccggagat gcgggcagtg
     6121 gtgtacgagc ggccggggcg ggtggcgttg cggacggtgc ccgacgcccg gatcgaacgg
     6181 ccgggcgacg cggtggtccg ggtcgccgcg gccggggtct gcgggagcga cctgatcttc
     6241 gtcggccgcg gcctctacca ggcggggcag cggtgcgggc acgagttcct cggcgtggtc
     6301 gaggagaccg gccgggcggt caccgcgctg cgggtcggcg acgcggtgct cgcgccgttc
     6361 tgctgggcgg acggcgactg ccggcactgc ctggccggcc tgctcacctc ctgcgcggac
     6421 ggcggggtgt ggggtctcgg cgaggggtcc gacgggggcc agggcgaggc cgtccgggtg
     6481 ccgcacgccg acgcgaccct ggtccggctg ccggcggagg cgctcaccga ccggcggctc
     6541 gccccggccg tgctcgccct cgccgacgtg ctgcccaccg gccatcacgc ggtggtcgcg
     6601 gccggcgtcc ggcccggctc gaccgtcgcc gtggtcgggg acggcgcggt cggcctgtgc
     6661 gccgtcctcg ccgccgcccg ggccggtgcc gagcggatca tcgccgtcgg cagccacccc
     6721 ggccggctgc ggctggccct ccgcttcggc gccaccgaca ccctcgccgc ccgcggcgcc
     6781 gacgccgacc ggcggctcgc cgaactcacc gacggccacg gcgtggacgc cgccgtcgag
     6841 gcggtcggca ccacccccgc gctggagacc gcgatccggg ccacccgccc gggcggcacc
     6901 gtcggcttcg tcggcctccc gcaccacgac accgcgcccg acctcggccc cgcgttcttc
     6961 cacaacgtgg ccctgcgcgg cggcatcgcc ccggtccgcc gctacctgcc cgagctgctg
     7021 cccgaggtgt gcgccgggag cctggacgcc ggggcgctgt tcgacttcac cggcaccctg
     7081 gccgacgtgc ccgccgcgta cgacgcgatg tccggccgca ccgtgctcaa ggccctgatc
     7141 cgcccctgaa gccgtcccct gaagccgtcc gctgaacccg cccgctgaga aggcccgcct
     7201 ggaggtgccc catgcccgcc gccgcccgcc cgccgggaac cgtccgggac gccgtcgtcg
     7261 acgtcctgcg ccaccaccgc gcgcacgtcc tgttcggcaa ccccggtacc accgaactcc
     7321 cgttcctcgc cgacctgccg cccgacctgc gctacgtcgc cgggctgaac gagtcctccg
     7381 tcaccgccat cgccgacggc tacgcccagg ccaccgggcg ccccgtcctg gtcaacctgc
     7441 acaccgcgcc cggcctcggc aacgcgatgg gctcgctggt caacgcgtac gcggcgggca
     7501 gcccgctggt cgtgctgacc gggcagcagc ggcgcgacct gatcggcgcc gacgccatcc
     7561 tgaccaaccg gaacccggtg ctgctgccgc tcggcgcggt caagcacgcc gaggagccgg
     7621 ccggcgcgca ggacgtcccg gcggcgctcg cccgcgcctt ccacctggcc gggctgccgc
     7681 ccaccgggcc cgtcgcggtg tcggtgccga tggacgactg ggaccggccg ctgcggcccg
     7741 cccccgccac caccgcgatc accacccggg tcaccggcgg cgccaccgcc cccgccgacg
     7801 tgctggagga actggccgac cggctcgccg ccgcccgctc cccggccctg gtggtcgggc
     7861 ccggcaccga cgaccggcgc ggctactggg cggtcgccga actcgccgac cggctcgccg
     7921 ccccggtctg ggtcaccccc tgggcccacc gcctcggctt ccccaccgac caccccggct
     7981 tcgtcggcca gctcccgctc gactccggcc cggccgccgc cgcgctggaa ccgcacgacc
     8041 tggtgctggt gctcggcggc ccggtgttcc gctacatgtt cgccgagcag tggcccgcgc
     8101 tcgcccccgg caccgaactc gtcgcgctga ccgacgaccc cgggcaggcg gcccgcaacc
     8161 tggccggcga gaccgtgctc tgcgacgtgc ccggcaccgt ccgcgccctc tgccgggcgc
     8221 tggccgaccg cggctccgaa cgcccgcccg gcccgccgcc cgcccggccg cccgccgagg
     8281 ccacccccgg ctacgcgcag gccgcgctcg cgctgcacca ggtgatgccg cccgaggcgg
     8341 tgctcgtcgt cgaacccccg cagtcctacg agtacctgct gcgcgcgctg ccccgcaccc
     8401 gccccggctc gctctacttc gccgccgcct gcggactcgg cttcgccctc ggggccgccg
     8461 tcggcatcca actcgccgaa cccgaacggc cggtggtcgc cctggtcggc gacggcgcgg
     8521 tccagtacaa cctgcaggcg ctgtggacgg ccgtcctcga acgcctcccg atcaccgtcc
     8581 tggtggtcaa caacggcggc tacggcatcc tcaaggactt cgccgaccgc ggccggctgt
     8641 ccgccgtccc cgccctcgac ctcccgcaca tcgacctgac cgccctggcc cgcggctacc
     8701 aggtccccgc cctccgcgtc cccgccgacc ccgcccggat cgccgccgcc ctgcgcaccc
     8761 ccctcccgcc cggcccgcgg ctgatcgagg tcgtcgtccc cgcccgctga aacagttcga
     8821 acccgtgaca acccttcgtc ggggccgccg cgtcagggaa gcggcggggt gatcgggacg
     8881 gcctttcacg agtaaagagt cataaaagaa actgttatga cgaagtgaaa ggttccgata
     8941 cgatctcccc gcccgccgcc gccgtgatca ccacggccgc ggcggctgat cctgcgcacg
     9001 ccacgtcccc tgggggagtt ttgagcatcc gtcggtcgat agccgccgcc gtcttcgtcg
     9061 cggccgtctc cgcacccacc gtcttcggca ccgcacaggc gatggccgag accaacccga
     9121 ccggccagtc cgtcccggac gccgtcctcc ccgacaccga gccggccccg gccccggccc
     9181 cggagtcccc ggtcgcggag tccccggccc cgagcccgac cgccgagtcc ccgagcccgg
     9241 ccgcggagtc cccggccccg agcccgaccg ccgagtcccc gagcccggcc gcggagtccc
     9301 cggccccgag cccggccgcg cccgctccgg cggaggccgc cccggccgcc cccgccgcgc
     9361 cggcccagcc cgccgctccg gccgcgcccg cccagccgtc cgcgtccgac gaggccgcgc
     9421 tgctggcccg gctggaggcg ctgatcgccg agccggggca ctcccggtac ttctacgagc
     9481 acgccgagga ggccaggggc cgcgacgccg agggcatccg gcacttcctc gacgtcgagg
     9541 cgcccaggat cttcctggag gaccggcggg tcgaggtggc ccaggccatc taccgcgccg
     9601 ggcagggcag cgacatcgcg aaggcgggca gcaaggcgct cgacgacggc agcccggagg
     9661 cgatcgagca cttcctgaac gtcaccctcc cggcgttgat cgtctccgac cagcgggtcc
     9721 agatctcgcg gctgatcgac gagccgggca tcggcgccaa gctgcgcgag gccgccctga
     9781 aggcgctgga ggacggtcgg cccgcggtgg tcaccgcctt catggcgaac ctgccgcagc
     9841 tccgcttcgc cgacgacctg ctgcgcgcgt cgaaggcgat gtcggacggc ggcccggagg
     9901 tccgcaaggc ggcgggcgcc gcgctcgacg cgggcacccc ccaggcgctg cgcgccttca
     9961 tcaccaccgg tctcgccgag gcccgggcca aggacaaggc ggcccaggag cagcagaacc
    10021 aggagcagca gaaccagaac cagggtcagg gtcagggcca gcagggcggc accaccgtca
    10081 ccccggcctc cgacacccgg gtggccaccg gcagcaccgg caccacggcc acgaccacca
    10141 ccaccgccac gaccggcgcc ggcagcaccg ccgcgcagct cgcgtccacc ggcacggacg
    10201 ccccggtcgg ctcgctcgcg ttcggcggcg ccgccgcgat cgcgctcggc gccggcgccc
    10261 tggtggtcac ccgccgccgc cgggaatcct gacccaccgt cagttccgtt gacggcggaa
    10321 cgagtcgatc cggccgccct cgcgcacccg cgagggcggc cggatcgcgt tgcgcgccaa
    10381 cggaactgac gccggggcat cggcgtcacc gcgtcgacca gcgcttctcg gatcggcggg
    10441 ctggcgggaa atcatctccc tgtgcgaccc gggaaggttc cgatacgatc gcccggttcg
    10501 accccgtccg ggcccgtccc gtccgggtcg ttttcgtacg atttgctttc catgggggaa
    10561 tcttgagcat tcgtcgttcg atagccgccg ccgtcttcgt ggccgcggtc gccgcgccca
    10621 ccgtgctcgg tgcgggcacc gcgatggccg agaccggcgc cgacccggtc ggccagtccc
    10681 agccggacgc cgcacccgcc gaggccgcgc ccgcgcccgc gcccgccgag gccgcacccg
    10741 ccgaggccgc gcccgccgag gccgcacccg cgcctgccgc gcccgcgccc gccgccgaac
    10801 ccgccgcgcg cgccgccgac tcgccctcgt ccgccgcgtc cggctccggc tcgtcctcgc
    10861 ccgacccgtc cccctccccg gacccgacgc cgaccccgga cccgacgccc agcggcggca
    10921 ccgagacccc ggacccgga
//
