LOCUS       NC_016109              10924 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Kitasatospora setae KM-6054, complete sequence.
ACCESSION   NC_016109
VERSION     NC_016109.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora setae KM-6054
  ORGANISM  Kitasatospora setae KM-6054
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1
  AUTHORS   Ichikawa,N., Oguchi,A., Ikeda,H., Ishikawa,J., Kitani,S.,
            Watanabe,Y., Nakamura,S., Katano,Y., Kishi,E., Sasagawa,M.,
            Ankai,A., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Nihira,T., Horinouchi,S., Ohnishi,Y., Hayakawa,M.,
            Kuzuyama,T., Arisawa,A., Nomoto,F., Miura,H., Takahashi,Y. and
            Fujita,N.
  TITLE     Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary
            snapshot of the family Streptomycetaceae
  JOURNAL   DNA Res 17 (6), 393-406 (2010)
   PUBMED   21059706
  REMARK    DOI:10.1093/dnares/dsq026
REFERENCE   2  (bases 1 to 8783278)
  AUTHORS   Ichikawa,N., Watanabe,Y., Nakamura,S., Katano,Y., Oguchi,A.,
            Ankai,A., Yashiro,I., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Ikeda,H., Ishikawa,J., Horinouchi,S., Ohnishi,Y.,
            Hayakawa,M., Nihira,T., Kitani,S., Kuzuyama,T., Arisawa,A.,
            Nomoto,F., Miura,H., Takahashi,Y. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2009) Contact:Director-General Department of
            Biotechnology National Institute of Technology and Evaluation, NITE
            Genome Analysis Center (NGAC), Department of Biotechnology; 2-49-10
            Nishihara, Shibuya-ku, Tokyo 151-0066, Japan URL
            :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000269985.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:32:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,713
            CDSs (total)                      :: 7,605
            Genes (coding)                    :: 7,487
            CDSs (with protein)               :: 7,487
            Genes (RNA)                       :: 108
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 25 of 118
            Pseudo Genes (incomplete)         :: 95 of 118
            Pseudo Genes (internal stop)      :: 9 of 118
            Pseudo Genes (multiple problems)  :: 11 of 118
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:33:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4923886
            Orig. end                         :: 4934810
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010968.1.
            'Otoguro, M. is at NITE Biological Resource Center (NBRC). Ikeda,
            H., Miura, H. and Takahashi Y. are at Kitasato Institute for Life
            Science. Ishikawa, J. is at National Institute of Infectious
            Diseases. Horinouchi, S., Ohnishi, Y. and Kuzuyama, T. are at The
            University of Tokyo. Hayakawa, M. is at University of Yamanashi.
            Nihira, T. and Kitani, S. are at Osaka University. Arisawa A. is at
            Mercian Corp. Nomoto F. is at Nagase & Co. Ltd. The other authors
            are at NITE Genome Analysis Center (NGAC).
            Please visit our web site. URL:http://www.bio.nite.go.jp/
            DOGAN ; Database of Genomes Analyzed at NITE. The database contains
            genome sequence data, physical maps and the genomic and proteomic
            data of the microorganisms analyzed.
            URL:http://www.bio.nite.go.jp/dogan/Top
            The microbial strain and microbial genomic DNA clones used for the
            sequencing project are available through the NBRC.
            URL:http://www.nbrc.nite.go.jp/e/index.html.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10924
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="25"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10924
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10924
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5924](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5924
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(1883..1892)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.04"
                     /tool="antismash"
     misc_feature    complement(1928..1942)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.67"
                     /tool="antismash"
     misc_feature    1929..1943
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.76"
                     /tool="antismash"
     misc_feature    complement(1932..1947)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    complement(2006..2020)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.71"
                     /tool="antismash"
     misc_feature    2007..2020
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.59"
                     /tool="antismash"
     misc_feature    complement(2007..2023)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.77"
                     /tool="antismash"
     misc_feature    complement(2008..2021)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.66"
                     /tool="antismash"
     misc_feature    complement(2009..2022)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.6"
                     /tool="antismash"
     misc_feature    2010..2023
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.23"
                     /tool="antismash"
     misc_feature    2010..2026
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.99"
                     /tool="antismash"
     misc_feature    2010..2028
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.83"
                     /tool="antismash"
     misc_feature    complement(2083..2096)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.19"
                     /tool="antismash"
     gene            2119..2709
                     /locus_tag="KSE_RS21970"
                     /old_locus_tag="KSE_44570"
     CDS             2119..2709
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137540.1"
                     /locus_tag="KSE_RS21970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_44570"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_014137540.1"
                     /transl_table=11
                     /translation="MQPRSSATRQALVRASVELIANGGLAEAGLVNICKTAGVSRGALY
                     HHFQSIAELVAEVHAQARERVLAMIEHSFAERDEAAPVRFSVALGQALAADQLVRAGML
                     VSVDGTDGPPRLREEMLEQLHGRVAAADPEAADQLLLADLAVVVTAGLESLGRTDAAWW
                     APERAERIWGRALAGSAALAGSAAVPEGAVQQP"
     gene            complement(2980..3636)
                     /locus_tag="KSE_RS21975"
                     /old_locus_tag="KSE_44580"
     CDS             complement(2980..3636)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137541.1"
                     /locus_tag="KSE_RS21975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_44580"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_014137541.1"
                     /transl_table=11
                     /translation="MAKQERGTRSRRAILEAAAEVFDLRGFDAASTNEILSRSGLTRGA
                     LYHHFPSKEAIAVALFEAHREGLTLPPHTSKLQAIVDLSFAFAARLQTDPVLRASVRLA
                     VEQTSFTRPANSPYDQSNAVIRQLLGEAATQGETLPGLDLDEATEVIVGAFTGMQVMSQ
                     VHSNRADLQQRLGSLWRLLLPGLANPGMICNLRLTPLPEAAAPEPEPAPEAPAVD"
     misc_feature    complement(3685..3694)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 15.81"
                     /tool="antismash"
     gene            complement(3827..4594)
                     /locus_tag="KSE_RS21980"
                     /old_locus_tag="KSE_44590"
     CDS             complement(3827..4594)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137542.1"
                     /locus_tag="KSE_RS21980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_44590"
                     /product="response regulator transcription factor"
                     /protein_id="WP_014137542.1"
                     /transl_table=11
                     /translation="MNLQTTGLPEPLAARHRTAPHTHRPAPGVHVPVEHRALRILVVQN
                     EERAAGALVQDLARRGYRAESVATGGQALRVHHRADLILLDLDLTDLDGLEVCGGIRAV
                     SDTPIIAVTERGSELDRVLGLQAGADDYMVKPYGFHELLARIGAVMRRFRPVRTSRTID
                     HGPLRIDASTRRVTLHGEWVELTRKEFDLLHALAAQPGAVVSRRQLMTQVWDDAWSPKG
                     RTLDTHVSSLRGKLGSSDWIVTVRGVGFRLGCP"
     misc_feature    complement(4606..4624)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.91"
                     /tool="antismash"
     misc_feature    complement(4752..4768)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     misc_feature    4865..4876
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.36"
                     /tool="antismash"
     misc_feature    4880..4891
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.06"
                     /tool="antismash"
     misc_feature    complement(4888..4903)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.33"
                     /tool="antismash"
     misc_feature    4888..4904
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.81"
                     /tool="antismash"
     gene            5001..5924
                     /locus_tag="KSE_RS21985"
                     /old_locus_tag="KSE_44600"
     CDS             5001..5924
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137543.1"
                     /locus_tag="KSE_RS21985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_44600"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_014137543.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-22, bitscore: 78.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDLLDTVRTGLTTIVPRQYVHRASVSEVLLTDWRAAPEPDAFVV
                     RAQWPRGHALFAQSGGYQDPLLLMESVRQAGMLLAHAEYGVQLGTRFLLQSIRFSAAAE
                     LLVATPAPTEVELRVVAHGITRRGFRVTGMRFEMTVLVDGVPLATAGAAVAFTTPAVYA
                     RLRGDLPTWIPRPAGPAVDPATVGRRQPENVVLGAHPEGGPGRWRLRVDCEHPIFFDHP
                     VDHVPGMLLIEGARQAAHAVTGRPDGLVVDLDSSFHSYAELDEFCGIEAERTGVDLAGD
                     AHVHVRGVQDGRTVFTTRLTLRDRTR"
     gene            5921..6883
                     /locus_tag="KSE_RS21990"
                     /old_locus_tag="KSE_44610"
     CDS             5921..6883
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (60-168). E-value:
                     1.8e-09. Score: 29.0. Matches aSDomain:
                     nrpspksdomains_KSE_RS21990_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137544.1"
                     /locus_tag="KSE_RS21990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_44610"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_014137544.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.8e-12, bitscore:
                     43.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAPGPRPPGRTLITGAAGFLGSHVARAAARHGGALVLAAHHRRP
                     APPSAAGVVVRADLGDPASLRGLCDGVQVLLHCASAVGGSPERNRAVNALGTAALLAEA
                     ARAGVERVVYVSTASVYGRGRYLGERPEQLTRRPGSPTSRTRAEAEEAVLAYGGTVLRP
                     HLVYGPGDRWVVPGLRRLLARLAVTPTGWRTRHSVIAADELGTLVAAVGHAPAGRLRSP
                     VYHLAHPEPVPAAALLAAAARCGPRPPSAPLTLEQARARVAADPAAAHALAMLADDHWF
                     DSTPLWTDLGRRPGRAPGTDLTQFKEWYPWSTPSSDAAA"
     CDS_motif       5957..6034
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KSE_RS21990_0001"
                     /evalue="3.40E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="KSE_RS21990"
                     /protein_end="38"
                     /protein_start="12"
                     /score="10.1"
                     /tool="antismash"
                     /translation="LITGAAGFLGSHVARAAARHGGALVL"
     aSDomain        6101..6424
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KSE_RS21990_NAD_binding_4.1"
                     /evalue="1.80E-09"
                     /label="KSE_RS21990_NAD_binding_4.1"
                     /locus_tag="KSE_RS21990"
                     /protein_end="168"
                     /protein_start="60"
                     /score="29.0"
                     /tool="antismash"
                     /translation="DPASLRGLCDGVQVLLHCASAVGGSPERNRAVNALGTAALLAEAA
                     RAGVERVVYVSTASVYGRGRYLGERPEQLTRRPGSPTSRTRAEAEEAVLAYGGTVLRPH
                     LVYG"
     gene            6850..7842
                     /locus_tag="KSE_RS21995"
                     /old_locus_tag="KSE_44620"
     CDS             6850..7842
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150657.1"
                     /locus_tag="KSE_RS21995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_44620"
                     /product="aldo/keto reductase"
                     /protein_id="WP_014137545.1"
                     /transl_table=11
                     /translation="MEYAKLGRSGLTVSRLVLGTMNFGTETDEAESHAIMQRAHEQGVN
                     FFDTANVYGTRPGETSTEEIIGRWFAGDPERRDRTVLATKVYIPTGSRPNEGRLSALNI
                     RRACEASLKRLRTDHIDLYQMHHIDRDTPWDEIWEALGVLRQQGKVLYFGSSNFAGWHL
                     AQAQEAARSRHFLGLVSEQTRYNLMTRWAELEVLPAARNYGIGLIPWGPLNGGVLGGVL
                     RKQREGGAGRGVSGHSAAALAQHRDTVEAYEKLCADLGADPAHVGLAWLLAQPGVTGPI
                     IGPRTVDQVDSSLRALDVRLDGSVLHRLDQLFPAPGPNGAKPAPEAYAW"
     misc_feature    8138..8153
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.66"
                     /tool="antismash"
     gene            8214..9637
                     /locus_tag="KSE_RS22000"
                     /pseudo=""
     CDS             8214..9637
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511390.1"
                     /locus_tag="KSE_RS22000"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDASVVVVGAGPAGLMLAGELRLAGADVTVLERHRARTGEPRGIG
                     LTTGTMEVFDQRGLLRRFEPHDTADTGHFGGVPFDPGVLAPHLRPARTIPQSATEQVLE
                     DWARELGADLRRGHDYLGHRDEGDTVTLTVRRDDGTEYELATRYLVGCDGGRSAVREDA
                     GFDFPGTAATTEMLLADLRGVDLEPRTTGEHAGGGFVTVAELPGGIHRIIVGEHGIPPR
                     RRTGPPAFEEVADLWKRLTGIDVSHAEPVRVSAFGNASRQVSAYRRGRVLLAGDAAHVH
                     LPAGGQGMDTSLQDSHNLGWKLASVLRGDAPEDLLDTYHAERHPVGRALLADTRAQSRP
                     VPGGEEAVPLREVLAELVGLPGATRHLAAEVSDKDIRYEVGGGANPLLGRRLPHLPLTT
                     ADGTGTTSTALLRGGRGVLLDLEDNPVLRRRARPTAPPRCWSARTATWPGPPPAATTTC
                     PARSPAGSAPPSGGAR"
     gene            9634..10599
                     /locus_tag="KSE_RS22005"
                     /old_locus_tag="KSE_44650"
     CDS             9634..10599
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (74-241). E-value: 9.1e-09.
                     Score: 27.4. Matches aSDomain:
                     nrpspksdomains_KSE_RS22005_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137548.1"
                     /locus_tag="KSE_RS22005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_44650"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_014137548.1"
                     /transl_table=11
                     /translation="MTRVDTEVPAVGAGPVGLLPAAEPRAGGARTISGPDTPAPPAPTA
                     PPAPGAPAAPRPYPTPQPGRSTVDRLTDKTALVTGSSRGIGRATAIRLAREGALVAVHW
                     TADRQAAEDTVALIEKEGGRAFAVRAELGVPGDVHTLFLGLERGLEERTGEARLNILVN
                     NAAVTPTGSAPEDLTPEEFDRYFAVNAKAPYFLTQRAAALMPDGGRIVNVSSAVTRTAA
                     PDQLAYAMTKGAIEQLTLHLALILAPRGITVNSVAPGITDNGGAIFANPDAVAAMSQLS
                     AFKRVGGAGDVADVITFLTTDEARWITGAFIDATGGTLLT"
     aSDomain        9856..10356
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KSE_RS22005_PKS_KR.1"
                     /evalue="9.10E-09"
                     /label="KSE_RS22005_PKS_KR.1"
                     /locus_tag="KSE_RS22005"
                     /protein_end="241"
                     /protein_start="74"
                     /score="27.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRATAIRLAREGALVAVHWTADRQAAEDTVALIE
                     KEGGRAFAVRAELGVPGDVHTLFLGLERGLEERTGEARLNILVNNAAVTPTGSAPEDLT
                     PEEFDRYFAVNAKAPYFLTQRAAALMPDGGRIVNVSSAVTRTAAPDQLAYAMTKGAIEQ
                     LTLH"
     CDS_motif       9862..9927
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KSE_RS22005_0001"
                     /evalue="4.90E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="KSE_RS22005"
                     /protein_end="98"
                     /protein_start="76"
                     /score="12.8"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRATAIRLAREGA"
ORIGIN
        1 ggtgccgcgg gccagcgtct cgggcgccgg ggcgcccggg ccccaggcgg ccgcgggcag
       61 cgggtcgagg gcgagcggtt cggcccgcca gccgtgcagg tactgccgtt cgtgccggac
      121 gccggtgacg cccttggtct cgccgagcag cggcccgtag ttgacccggc cgagcgattc
      181 gaccagcagg ctgatccggg ccccgccgcc ggccgcgacc ggttcggcgg cgaccccgcg
      241 ctccagcgag gcgacgtgcc tcccgtcgac gcgcaggccg gcccggtcgc gcaacccctc
      301 gacggtgagc ggcagttcgg ggctgagcgc gggcaccgag gcggtgtagc ggaccaggcc
      361 gtgctccagc ccgagctcct cgaacagcgg cggcacgggc gactccacgg cgggcccgaa
      421 cggcagcgcg gcgtactcgt cgaggagcac ctcgcgggcc gccagcaccg gcgcggggga
      481 ggggagttcg ggagtgcgcc cgtccaggtg gcgtgcctcg tccgggtgtt cggcggcgaa
      541 ctcggcgagc acctgccgga acgcctcgaa cttggcggtg ggcaggccgc gttcgtccag
      601 cggggcgtcg tagtcgtagg aggtgacggt cggctggtac ggggagtgcg tccagtcggt
      661 ggagttgaac ggcgggtcgg cgtggttggc gcccgcggag gtgccgaagc tggtgccgcc
      721 gtgggccatg tacaggttga ccgagccgcc cgccgcgagg atccggcgca ggctgtcggc
      781 cgcgtcgtcg gcgccccggg tgtggtgcgg gcggccccac tggtcgaacc agccgttcca
      841 gaactccatg cagaacggcg ggtcctcggg gcggtggcgg cgcagcgcgg cgaagccgcg
      901 ctccgggtcg gagccgaagt tgacggtcgc cagcaccccg gggacggtgc cggccgccag
      961 catgtggtcc tccggcccgt ccgaggtgaa cagcggcacg ctcacgccgc gctcgcgcag
     1021 cccccgggcc agcgcggcca ggtagccggc gtcgctgccg aacgcgccgt actcgttctc
     1081 cacctgcacc atcagcacgc tgccgtccgg ccgcccccac tgccgctcca ccacctgcgg
     1141 cagcagcacg tcgaagaacg cccccaccgc cgccaggaac tccgggtcgc cggtgcgcac
     1201 ccgccgcccc agccgggcgg tgagccagcc gggcagcccg ccgttgtccc actcggcgca
     1261 gatgtacggg ccggggcgga cgatcgtcca caggccctgc cgccgggcct cgtccaggaa
     1321 cgcgcccagc tcgccgaccc gggcgaaccg gcccggcgcg ggctcgtgca ggttccacgg
     1381 cacgtaggtc tcgaccgtgt tcagccccat cgcccgcacc gccgcgagcc ggtcggccca
     1441 ctgctcgggg cggctgcgga agtagtgcac cgcaccggac aggacgcgca gcgggcggcc
     1501 gtccaggcgg aaaccggtcg agtcgtaggt gagcatgccc ctagcctgcc caaaccggcg
     1561 gccggtagcc gccaggacat gcgcggggcc ggtcggcggg cgcggtcggg tgcggtcggc
     1621 ggacgcggtc ggcgagggcg tgcggcgggg gtggtcggcg gggacctgat ttcggggccg
     1681 ggcgtgcggc ggattagtct cggggggtgg acccgaagac ccgtatgcgg atcgtcaccg
     1741 gcgcgttcgt cctgctgctc gccgtggtga tcgtcagcgc gctcctgcag aagtagccgc
     1801 agcggcccag ccgctccgcc ccaccggccc cgcctgccga cggcggcctc ctcccgcccc
     1861 tcgacgcggg agttctcccc tcgcagaaaa catacagaca tgtcggtcta tgtcagagga
     1921 atgtcgacac tgtcaagaaa atgtccgggg acatacaggt caagcgtggt atttgccgcc
     1981 ctgttgtgcc ctgagtacgc tctgggatgt tctgtaaaaa caggcagggt tatatggtta
     2041 gttggggttg agcaccgggg atccagggtg ctcgtctccg ttcgaccctt cgttcgttcc
     2101 gacgaggagg caggcaggat gcagccgcgc tccagcgcca cccggcaggc actcgtacgt
     2161 gcctcggtgg aactcatcgc gaacggaggg ctggccgagg ccggcctcgt gaacatctgc
     2221 aagaccgccg gcgtgagccg cggcgcgctg taccaccact tccagtcgat cgccgagctg
     2281 gtcgccgagg tgcacgcgca ggcgcgggaa cgggtgctgg cgatgatcga gcacagcttc
     2341 gccgagcggg acgaggcggc gccggtccgg ttcagcgtcg cgctcggcca ggccctggcg
     2401 gcggaccagt tggtgcgcgc cggcatgctc gtctcggtcg acggcaccga cgggccgccc
     2461 cggctgcgcg aggagatgct ggaacagctc cacggccggg tcgccgccgc cgacccggag
     2521 gccgccgacc aactgctgct cgcggacctc gcggtggtcg tcaccgccgg cctggagtcg
     2581 ctcggccgca ccgacgccgc ctggtgggcg cccgagcgcg ccgagcggat ctggggccgg
     2641 gcgctggccg gttccgccgc gctggccggt tcggccgcgg tgccggaggg cgcggtgcag
     2701 cagccgtagc gcggcggggc cggtgctgcc gggccggcgg tgccggggcg gcgaacggcg
     2761 gtgccccgta cgggcgttgg cgactcgggg ccggcgcggt acggggcacg gggttctccc
     2821 ggccgggcgg ttcggcgggt gccgggccgc cggggcgggg cgctgcgggc ggggcgctgc
     2881 ggggtggtgt gcgcggtgcg cggtgctgcg atgcggtggc gtccggtccg gggtcggggc
     2941 ccggggtccg gcggtgtcgt cccggcggtg cggtgcgggt cagtcgacgg cgggggcttc
     3001 gggggcgggt tcgggctcgg gggcggcggc ttcgggcagc ggggtcagcc gcaggttgca
     3061 gatcatgccg gggttggcca ggccgggcag cagcagccgc cacagcgagc cgaggcgctg
     3121 ctgcaggtcg gcccggttgc tgtggacctg ggacatcacc tgcatgccgg tgaacgcgcc
     3181 gacgatcacc tcggtggcct cgtccaggtc gagtccgggg agcgtctcgc cctgggtggc
     3241 ggcctcgccg agcagttggc ggatgaccgc gttggactgg tcgtagggcg agttggccgg
     3301 gcgggtgaag gaggtctgct cgaccgcgag ccgcacgctg gcgcgcagca ccgggtcggt
     3361 ctggagccgg gcggcgaagg cgaaggagag gtcgacgatc gcctgcagct tgctggtgtg
     3421 cggggggagc gtcaggccct cgcggtgcgc ctcgaagagg gccacggcga tggcctcctt
     3481 ggaggggaag tggtggtaga gagcgccgcg ggtcagtccg ctccggctga ggatctcgtt
     3541 ggtgctggcg gcgtcgaagc cgcgcaggtc gaatacctcg gccgcggctt ccaggatcgc
     3601 ccgccgggat ctcgtgccgc gttcctgctt cgccatacct gtgtccttgt gctcgctgtg
     3661 ctgtggatgc ttccggtgtc gagggaaaaa aatatgccga cctgtctgca tcctagtggc
     3721 acgcaggcag gtcggcggag tccggtgttc cgggcggcgg tgcggccggg ggcgtcgtcg
     3781 gcccggcggg ggagggggcg gggcaccggg gtggcggtgc gccggctcac ggacagccca
     3841 gccggaaccc cacgccgcgg acggtcacga tccagtcgct ggaccccaac ttgcctcgca
     3901 ggctgctgac atgggtgtcg agggtgcgtc ccttgggcga ccaggcgtcg tcccagacct
     3961 gggtcatcag ctgccgccgg gagaccaccg cgccgggctg cgccgcgagc gcgtgcagca
     4021 ggtcgaactc cttgcgggtg agctccaccc actcgccgtg cagggtcacc cggcgggtcg
     4081 aggcgtcgat ccgcagcggg ccgtggtcga tggtgcggga ggtccgcacc gggcggaatc
     4141 ggcgcatcac ggccccgatc cgggccagca gttcgtggaa gccgtacggc ttgaccatgt
     4201 agtcgtcggc gccggcctgg aggccgagga cccggtcgag ctcgctgccg cgctcggtga
     4261 cggcgatgat cggggtgtcg ctgaccgccc ggatgccgcc gcagacttcg aggccgtcca
     4321 ggtcggtgag gtccaggtcg agcaggatca ggtcggcccg gtggtggacg cgcagggcct
     4381 ggccgccggt ggcgacgctc tcggcccggt agccgcgccg ggccaggtcc tggaccaggg
     4441 cgccggcggc gcgttcctcg ttctgcacca ccaggatgcg cagcgcccgg tgctcgaccg
     4501 ggacgtgcac tcccggggcc gggcggtggg tgtgcggtgc ggtgcggtgc cgtgcggcga
     4561 gcggctcggg caggcccgtc gtctgcaggt tcatctgtcc ccctgtctgc caggtgttcg
     4621 catggaaccc gggcgtgctt cgacgttacc aaacagactt gtctggatgt caatcgcaca
     4681 tcgggtcgtc gcaggtggcg gccgagtccg ccgggacgcc ccggcctgcc gggggcgaga
     4741 tgtggatgct atgcaccgat ttggtgcgga ttggttgatc atgagcgtgt tcgagccgtc
     4801 gccgggcgtg tcgagagggc cgctgggctg cgcgggaggc gggatggcga ggtggcgggc
     4861 cgaggaaaaa cattacggcg aaactgtttt tacttgtgtt acccgacagg tcggtctgta
     4921 tcttatggct gagcgggggc tggctgcgga cacgcagccg atgggtcggc cctgcccaga
     4981 atcagacagg agcgctgagg atgaccgatc ttctcgacac cgtccggacc gggctcacca
     5041 cgatcgttcc gcgtcagtac gtgcaccgcg cctccgtatc ggaagtgctg ctcacggact
     5101 ggcgcgccgc accggagccc gacgcgttcg tggtgcgggc ccagtggccg cgcgggcacg
     5161 ccctgttcgc gcagtccggc ggctaccagg acccgttgct gctgatggag tcggtccggc
     5221 aggccgggat gctgctcgcg cacgccgagt acggggtcca gctcggcacc cggttcctcc
     5281 tgcagtccat ccgcttctcg gccgccgccg agctgctcgt cgccaccccc gcccccaccg
     5341 aggtcgaact gcgggtggtc gcccacggga tcacccgccg cggcttccgg gtcaccggca
     5401 tgcgcttcga gatgaccgtg ctggtggacg gcgtgccgct ggccaccgcg ggcgcggccg
     5461 tcgccttcac caccccggcg gtgtacgccc ggctccgcgg cgacctcccc acctggatcc
     5521 cgcggcccgc cgggccggcg gtggacccgg cgacggtcgg ccgccgccag cccgagaacg
     5581 tggtgctcgg cgcgcacccg gagggcggcc ccggccgctg gcggctgcgg gtcgactgcg
     5641 agcacccgat cttcttcgac cacccggtcg accacgtccc cggcatgctg ctgatcgagg
     5701 gggcccggca ggccgcgcac gccgtcaccg gccggcccga cggcctggtg gtcgacctgg
     5761 acagctcctt ccactcctac gccgagctcg acgagttctg cggcatcgag gccgagcgga
     5821 cgggcgtcga cctggccggc gacgcccacg tccacgtgcg cggcgtccag gacggccgga
     5881 cggtgttcac cacccgcctg accctgcgcg accggacccg gtgaacgccc ccggaccccg
     5941 gccgccgggc cgcaccctga tcaccggcgc ggccggcttc ctcggctccc acgtcgcccg
     6001 cgcggcggcc cgccacggcg gcgccctggt cctcgccgcc caccaccgcc ggcccgcgcc
     6061 gccgtccgcc gccggggtgg tggtgcgggc cgacctcggc gacccggcgt cgctgcgcgg
     6121 cctgtgcgac ggcgtccagg tgctgctgca ctgcgcctcg gcggtcggcg gcagccccga
     6181 gcggaaccgg gccgtcaacg ccctcggcac cgccgcgctg ctcgccgagg ccgcccgggc
     6241 cggcgtcgag cgcgtcgtgt acgtcagcac cgcctccgtc tacggccgcg gccgctacct
     6301 cggcgagcgg cccgaacagc tcacccgccg ccccggctcg ccgacctcgc gcacccgcgc
     6361 ggaggccgag gaggcggtcc tggcgtacgg cggaacggtg ctgcgcccgc acctggtgta
     6421 cgggccgggc gaccgctggg tggtccccgg actgcggcgg ctgctggccc ggctggccgt
     6481 caccccgacc ggctggcgga cccggcactc ggtgatcgcc gcggacgaac tcggcaccct
     6541 ggtggccgcg gtggggcacg ccccggccgg acggctgcgc tcgcccgtct accacctggc
     6601 ccacccggag ccggtgccgg ccgccgcgct gctggccgcc gccgcccgct gcggaccccg
     6661 cccgccgtcc gccccgctca ccctcgaaca ggcccgcgcc cgggtggccg ccgacccggc
     6721 ggccgcccac gcgctggcca tgctcgccga cgaccactgg ttcgacagca cgccgctctg
     6781 gacggacctg ggccgacgcc cgggccgcgc ccccggaacg gacctcaccc agttcaagga
     6841 gtggtaccca tggagtacgc caagctcgga cgcagcggcc tgacggtctc ccggctggtg
     6901 ctcggcacga tgaacttcgg caccgagacc gacgaggccg agagccacgc gatcatgcag
     6961 cgcgcccacg aacagggcgt caacttcttc gacaccgcca acgtctacgg cacccgcccg
     7021 ggcgagacca gcaccgagga gatcatcggc cgctggttcg ccggcgaccc ggagcgccgc
     7081 gaccggaccg tgctggccac caaggtctac atcccgaccg gcagccggcc caacgagggc
     7141 cggctgtccg cgctgaacat ccggcgcgcc tgcgaagcct cgctgaagcg cctgcggacc
     7201 gaccacatcg acctctacca gatgcaccac atcgaccggg acacgccctg ggacgagatc
     7261 tgggaggcgc tcggcgtcct gcgccagcag ggcaaggtgc tgtacttcgg ctcctccaac
     7321 ttcgccggct ggcacctggc gcaggcccag gaggcggccc gctcccggca cttcctcggc
     7381 ctggtcagcg agcagacccg gtacaacctg atgacccgct gggccgaact cgaggtgctg
     7441 cccgccgccc ggaactacgg catcggcctg atcccgtggg gcccgctgaa cggcggcgtg
     7501 ctcggcgggg tgctgcgcaa gcagcgcgag ggcggcgccg gacgcggcgt cagcggccac
     7561 tccgccgccg cgctcgccca gcaccgcgac accgtcgagg cgtacgagaa gctctgcgcc
     7621 gacctgggcg ccgacccggc ccacgtcgga ctggcctggc tgctggccca gcccggcgtc
     7681 accggcccga tcatcggccc gcgcaccgtc gaccaggtgg acagctcgct gcgcgcgctc
     7741 gacgtccggc tcgacgggtc ggtgctgcac cggctcgacc agctgttccc ggcgcccggc
     7801 ccgaacggcg ccaagcccgc gcccgaggcg tacgcctggt aaccccgcca ccgccaccgc
     7861 ccaccccggg accggaggcg gtcgaggtgt gcgggcccgc cccgcacccg gccgagcggc
     7921 gcgagccgcc ggccccccga ggcccgccgg ccggtcgcct tcggccggtc gcggacccgc
     7981 agggaggccc acctgaaggg caccggcacc ggcaccggcg ccccgcccct cccgtccggc
     8041 tggaccgtcc gcgacgtgct cgtcggaccc cggcactccg ccgccaccgc cctggcgggc
     8101 gtcgcggagc cgctcatcac gctgcgctcg ctcccggtgg cgtgcctgtt cgacggcgcg
     8161 gcgggactgc cgctgtcggc ggccccgccg ccggccctga gaaggagaga ttcatggacg
     8221 cttcggtggt cgttgtcgga gcaggaccag ccgggctgat gctcgccggg gaactccgcc
     8281 tggcgggcgc cgacgtgacg gtgctggaac gccaccgcgc ccgcaccggc gagccccgcg
     8341 ggatcggcct gaccaccggc accatggagg tgttcgacca gcgcggcctg ctgcgccggt
     8401 tcgagcccca cgacaccgcc gacaccggcc acttcggcgg cgtcccgttc gacccgggcg
     8461 tgctcgcccc acacctgcgg ccggcccgca ccatcccgca gtcggcgacc gagcaggtcc
     8521 tggaggactg ggcccgcgag ctcggcgccg acctgcggcg cggccacgac tacctcggcc
     8581 accgcgacga gggcgacacc gtcaccctga cggtccgccg ggacgacggc accgagtacg
     8641 aactcgccac ccgctacctg gtcggctgcg acggcggccg cagcgcggtg cgcgaggacg
     8701 ccgggttcga cttccccggc accgccgcca ccaccgagat gctgctggcc gacctgcgcg
     8761 gcgtcgacct cgaaccccgg acgaccggcg aacacgccgg cggcggcttc gtgacggtcg
     8821 ccgagctgcc cggcgggatc caccggatca tcgtcggcga gcacggcatc ccgccccgcc
     8881 gccgcaccgg cccgcccgcc ttcgaggagg tcgccgacct gtggaagcgg ctcaccggca
     8941 tcgacgtctc gcacgccgaa ccggtccggg tcagcgcctt cggcaacgcc tcccggcagg
     9001 tctccgcgta ccggcgcggc cgggtcctgc tggccggcga cgccgcccac gtgcacctgc
     9061 cggccggcgg ccagggcatg gacaccagcc tccaggactc ccacaacctc ggctggaaac
     9121 tggcctcggt cctgcgcggc gacgcccccg aggacctgct cgacacctac cacgccgaac
     9181 ggcacccggt cggccgggcg ctgctcgccg acacccgcgc gcagagccgg ccggtgcccg
     9241 gcggcgagga ggccgttccg ctgcgcgagg tgctcgccga actggtcggc ctccccggcg
     9301 ccacccgcca cctggccgcc gaggtcagcg acaaggacat ccgctacgag gtcggcggcg
     9361 gcgccaaccc gctgctcggc cgccgcctgc cgcacctgcc gctcaccacc gccgacggca
     9421 ccgggaccac cagcaccgcg ctgctgcgcg gcggccgcgg cgtcctgctc gacctggagg
     9481 acaacccggt gctgcgccgc cgggcccggc cgacggcacc gccgcgctgc tggtccgccc
     9541 ggacggccac gtggcctggg ccgcccccgg cagccaccac gacctgcccg gcgcgctcgc
     9601 ccgctggttc ggccccgccc tcgggcggcg cccgtgaccc gcgtcgacac cgaggtgccg
     9661 gcggtcggcg ccggaccggt cgggctcctc cccgccgccg aaccgcgggc cggcggcgcc
     9721 cgcaccatca gcggccccga caccccggcg ccgcccgcgc ccaccgcccc gccggcgccc
     9781 ggcgcgcccg ccgcgccccg cccgtacccc accccgcaac ccggaaggtc gaccgtggac
     9841 aggctcaccg acaagacggc actcgtgacc ggctcctccc ggggcatcgg ccgggccacc
     9901 gcgatccggc tcgcccgcga gggcgccctg gtcgcggtgc actggaccgc cgaccggcag
     9961 gccgccgagg acaccgtggc cctgatcgag aaggagggcg gccgggcctt cgcggtccgc
    10021 gccgaactgg gcgtccccgg cgacgtccac accctcttcc tcggcctcga acgcggcctc
    10081 gaggagcgca ccggcgaggc ccggctgaac atcctggtca acaacgcggc cgtcaccccg
    10141 accggctccg cccccgagga cctcaccccc gaggagttcg accgctactt cgcggtcaac
    10201 gccaaggccc cgtacttcct cacccagcgc gccgccgccc tgatgcccga cggcggacgg
    10261 atcgtcaacg tctcctccgc ggtgacccgc accgccgccc ccgaccagct cgcctacgcc
    10321 atgaccaagg gcgcgatcga gcagctcacc ctgcacctgg cgctgatcct cgccccgcgc
    10381 gggatcaccg tgaacagcgt cgcccccggc atcaccgaca acggcggcgc gatcttcgcc
    10441 aacccggacg ccgtcgccgc gatgtcccag ctgtccgcct tcaagcgggt cggcggcgcc
    10501 ggcgacgtcg ccgacgtcat caccttcctc accaccgacg aggcccgctg gatcaccggc
    10561 gccttcatcg acgccaccgg cggcaccctg ctcacctgag gaggcccccg cacgtcgaac
    10621 cgcgtccagt cggcggtggg cctcgcccgc gccgagccgg acctgaccgc ggcctgaccg
    10681 cgtcccgggc ccggcccgtt ccggggcggg gcggggcggg gcgggtcgtt caggggcggg
    10741 ccggcccgct ctcgggctcg acgaagacca ccggcacgtt gttgtccagc accacgcggc
    10801 cgagcagttc ggcgagggat tcctgctccc gggggtcgag cccgtccgcc agccgctcga
    10861 tccggccgca cgcctcggtg tagaagcggt cggcgagccg ggcgcccttc ggggtgagcg
    10921 cgac
//
