LOCUS       NC_016109              11041 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Kitasatospora setae KM-6054, complete sequence.
ACCESSION   NC_016109
VERSION     NC_016109.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora setae KM-6054
  ORGANISM  Kitasatospora setae KM-6054
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1
  AUTHORS   Ichikawa,N., Oguchi,A., Ikeda,H., Ishikawa,J., Kitani,S.,
            Watanabe,Y., Nakamura,S., Katano,Y., Kishi,E., Sasagawa,M.,
            Ankai,A., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Nihira,T., Horinouchi,S., Ohnishi,Y., Hayakawa,M.,
            Kuzuyama,T., Arisawa,A., Nomoto,F., Miura,H., Takahashi,Y. and
            Fujita,N.
  TITLE     Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary
            snapshot of the family Streptomycetaceae
  JOURNAL   DNA Res 17 (6), 393-406 (2010)
   PUBMED   21059706
  REMARK    DOI:10.1093/dnares/dsq026
REFERENCE   2  (bases 1 to 8783278)
  AUTHORS   Ichikawa,N., Watanabe,Y., Nakamura,S., Katano,Y., Oguchi,A.,
            Ankai,A., Yashiro,I., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Ikeda,H., Ishikawa,J., Horinouchi,S., Ohnishi,Y.,
            Hayakawa,M., Nihira,T., Kitani,S., Kuzuyama,T., Arisawa,A.,
            Nomoto,F., Miura,H., Takahashi,Y. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2009) Contact:Director-General Department of
            Biotechnology National Institute of Technology and Evaluation, NITE
            Genome Analysis Center (NGAC), Department of Biotechnology; 2-49-10
            Nishihara, Shibuya-ku, Tokyo 151-0066, Japan URL
            :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000269985.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:32:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,713
            CDSs (total)                      :: 7,605
            Genes (coding)                    :: 7,487
            CDSs (with protein)               :: 7,487
            Genes (RNA)                       :: 108
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 25 of 118
            Pseudo Genes (incomplete)         :: 95 of 118
            Pseudo Genes (internal stop)      :: 9 of 118
            Pseudo Genes (multiple problems)  :: 11 of 118
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:33:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2543130
            Orig. end                         :: 2554171
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010968.1.
            'Otoguro, M. is at NITE Biological Resource Center (NBRC). Ikeda,
            H., Miura, H. and Takahashi Y. are at Kitasato Institute for Life
            Science. Ishikawa, J. is at National Institute of Infectious
            Diseases. Horinouchi, S., Ohnishi, Y. and Kuzuyama, T. are at The
            University of Tokyo. Hayakawa, M. is at University of Yamanashi.
            Nihira, T. and Kitani, S. are at Osaka University. Arisawa A. is at
            Mercian Corp. Nomoto F. is at Nagase & Co. Ltd. The other authors
            are at NITE Genome Analysis Center (NGAC).
            Please visit our web site. URL:http://www.bio.nite.go.jp/
            DOGAN ; Database of Genomes Analyzed at NITE. The database contains
            genome sequence data, physical maps and the genomic and proteomic
            data of the microorganisms analyzed.
            URL:http://www.bio.nite.go.jp/dogan/Top
            The microbial strain and microbial genomic DNA clones used for the
            sequencing project are available through the NBRC.
            URL:http://www.nbrc.nite.go.jp/e/index.html.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11041
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="17"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11041
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11041
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6041](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6041)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(360..2696)
                     /locus_tag="KSE_RS11265"
                     /old_locus_tag="KSE_22940"
     CDS             complement(360..2696)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135430.1"
                     /locus_tag="KSE_RS11265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_22940"
                     /product="MMPL family transporter"
                     /protein_id="WP_014135430.1"
                     /transl_table=11
                     /translation="MLHRLSRTVTLRPRTVLLFALVFLINALVVGAGVTDRLHSGGTTD
                     PSSESAHAATLLEQSFPGGSPNLVLEVTAKDGGSVDRPATARAGARLGERLAFEPGIAG
                     VGSYWQTGAVELRSADHGKALIVARIEGGEDRARAVFDRIAPHYRGSPADLPELDVRLG
                     GTVAVREQMQGTIAEDLARAELIALPVTLLILVLVFRGLTAALLPLVVGICAILGTNAA
                     LRVITSFAEVSVFAQNLTTALGLGLAIDYALLVVRRFREERDRGSEVREAVATTLRTAG
                     RAVLFSALTVAVSLSAMLVFPLYFLRSFAYAGISVVLIAAAAALIVLPALLTLLGHRID
                     SLRIPLPRRPRRPAAPDGGPGAGWYRVATAVMRRAPLFALAVTALLLAAGAPFLRVQFA
                     AADDRQLPAGTEARVVQQAVRDEFTAGATGAVDVIVRVTATATGTVTGPDRAALADYAA
                     KLSALPGVTEVATPLGPYRAGAPAGAAGPADSVRTADGYGHLTVTPADRRGDVSQANQD
                     LVGEIRRAGQGFPALRPAVGGQAAGLVDAKHAIAVRLLPAGALIAVATLVLVFLLTGSL
                     LIPLQAVLLNALSLTAMFGVVVWVFQEGHLSGVLGFTPTGSVETTLPVLMFCVAFGLSM
                     DYGVFLLARVKEERDRGADPHGAVAAGIRHTGGLITAAAVILSVVLIAIGTSRITNTKM
                     LGLGVALAVLVDATVVRCVLVPAVMALGGRATWWAPAPLRRFQERFGLREEARPAPAGP
                     APAADPAPAEPAPAATPAAAGKETR"
     gene            complement(2705..4336)
                     /locus_tag="KSE_RS11270"
                     /old_locus_tag="KSE_22950"
     CDS             complement(2705..4336)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135431.1"
                     /locus_tag="KSE_RS11270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_22950"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_014135431.1"
                     /transl_table=11
                     /translation="MSAAPEVLVAGAGPTGLTAACTLLQHGVPVRLVDRRACFTPEPKA
                     LVMWSGALEALHRLGVADALAERALPLNGASYWSGGRRLAGVRFGGLSGTRFPRPLCVP
                     QPVTEELLYERLLDLGGRVEWGTAVTAVATADGRAEVALTRADGTAETVSVPWLIGADG
                     TRSTVRESIGVPYPGSSHDREFVLGDGRIDGDLPPDEAQYHLSPDGVLVLVALPDGGHR
                     IFFDLPPGTRTGPPDDAELQQLLDARGHGRWTVRETWWTSRFRVHTKVAERFRHGPVLL
                     AGDAAHAHSPAGGQGLNTGVQDGFDAGWKLAAVLRGADPALLDSYDPERRPVSARAVRN
                     SERQTRLWLLRPRPARALRDLLLGTLARTGALERRIVPELAQLDLDLTASPAVRPTTVP
                     GAPVPGRRLGDAPLRPLRGTSATSLHDYLATGRHTVLIAGDGPAAADAAERATAELTLR
                     GLPDLTDVLLLLPPGTAADLPAGPANPDIAAGTLPGHPPTPGPALACLLRPDGVVAGAV
                     ALDRPDGVDALLSALPTGQRSAAPAP"
     gene            complement(4333..5010)
                     /locus_tag="KSE_RS11275"
                     /old_locus_tag="KSE_22960"
     CDS             complement(4333..5010)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135432.1"
                     /locus_tag="KSE_RS11275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_22960"
                     /product="HAD family phosphatase"
                     /protein_id="WP_014135432.1"
                     /sec_met_domain="HAD_2 (E-value: 7.5e-11, bitscore: 41.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTPGHPPVGPVDTVVFDYGGVLTNPLPETFAAFGHRTGIGTAD
                     IAKAFAAATERHGISPMAELEVAAITEREFTERLLAELPPGSEAVLGGRPFGELWFLGR
                     RANDRLVDYARKLRADGHRIALLTNNVVEWGPRWRATLPVDELFDAVVDSSAERVRKPD
                     PELYRRLLDRLGTTAERCLLVDDTAENCVAAERLGFKAVLFQDDAQAVRDVDALLAAGA
                     GER"
     gene            complement(5001..6041)
                     /locus_tag="KSE_RS42080"
                     /old_locus_tag="KSE_22970"
     CDS             complement(5001..6041)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003886.1"
                     /locus_tag="KSE_RS42080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_22970"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_014135433.1"
                     /sec_met_domain="AfsA (E-value: 5.7e-17, bitscore: 60.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAIPDVLPRDPQEHGRERPRPQADLRFDATVPRALVHRAAVAEV
                     FVTDSARITEAETADDRHTFEVAAQLPRGHVVGEHGDSYDFLLLVEVLRQSGVLVAHRY
                     LDVPLDRAFIFSALNLDVVDLEALRIGRRPAHAVLSTTVSPQRNGGGRVLGFDFTGTLW
                     LDGEPAIVGSGALTFVSSRAFQMLRAGNRKKLDGRSPRHPQLLRSEPALVGRRDEANTV
                     LTEPSVSDSGTASALLVVDTGHPHLFDHQLDHVPGNLQLEAARQLSVAAVAKLHGLPAE
                     SLVVTAARAAFTDFAELDLVTRAVARVGEAGFDGGLGAVTVPVEITVSQEGLPVATLSI
                     EVTQWS"
     misc_feature    complement(6034..6045)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     misc_feature    complement(6047..6064)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.44"
                     /tool="antismash"
     misc_feature    complement(6204..6219)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            6277..9483
                     /locus_tag="KSE_RS38310"
                     /old_locus_tag="KSE_22980"
     CDS             6277..9483
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF037660.5"
                     /locus_tag="KSE_RS38310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_22980"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_158413054.1"
                     /transl_table=11
                     /translation="MSTDDNEAGAMAGGTWFEAIGPVRVRRGPAELDLGPPRQRAVLTV
                     LILNHRRPVSTSRIVELVWGADAPDRGVNLVQKYVSGLRRVLQGVAELSWTEAGYVLVP
                     DGTVDVERFDALLTAGRTARAGGDPAAAAAALAEAVALWRGPLAQGMGGPGIEPLRLRL
                     TESWLAAKEDLFDAELELGRLPDRLPELRRLTAEHPLRERFRAQLVLALAQAGRPAEAL
                     AEHDRARRRLADEHGLDPGPHLRAAQEQVLRMASDRAAPADPATPPALSSPAALSSPAA
                     LPPAQLPPAPRVFVGRTALTARLAELLAPTGPPPLVCLEGTAGVGKSALALRAAHRAAG
                     LFPDGQLFADLRGFGRGLPADPSDVLGGFLRALGVPAERLPQDLGERIGLYRSILAGRA
                     VLVVLDDARDADQIRPLLPSGNRCAVLVTGRRRMISLTVREGAHRLIVPVLTAEESRGL
                     VNSLLGPGQTRDTAAVDRIIALCSGLPLALRIVTANAAHRADLPLARIARELARDRVAA
                     LSVPDEAQTALDCSIGLSYDLLPPGHRELFCLLGLVPGPDFTLRAATAMAGAGAAATER
                     GMRDLEVANLLERTADRYRFPHELLQLFAQERAHREVPAARRDEALHRLLGHYLAAVLR
                     AEGRDRRAAGADLPALPASSEPSDPSDPSDLSDLSDLSDPDFPDADLEEDDLESEFDNL
                     MATVLHAATAGPYPPVWQLADALRPFCKQHARTAQWSRLAGAGLAAARRAGDRRAEAAM
                     LLNLSDAEHNSGLVEEGAEHARQALALGRGLGLGRIEASALDQLGRAAWIRGALETART
                     LLTAAVDAHTRTGDRLGLAVSLSALGRTEFDAGHTEQAALHCSQVLRLARTIGAAAVET
                     MALVEMGVLHAALGRPENAARSLRAALALARDGGHRRAEALALARLSLLRAGGDRPREA
                     LAEARNALHVAEAHGDRWIEAECLNTTARTALATGDTDTAAARHRAALTLATRLGYRRA
                     ELQALLGLAETALRRGDTQRAADYAGRARAAAAGLGHLPLAAEALRIEGRLRATPDAAP
                     DPEPATAPEPASADRAQLGC"
     misc_feature    9555..9568
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.62"
                     /tool="antismash"
     gene            9598..10254
                     /locus_tag="KSE_RS11290"
                     /old_locus_tag="KSE_22990"
     CDS             9598..10254
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140546.1"
                     /locus_tag="KSE_RS11290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_22990"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_014135435.1"
                     /transl_table=11
                     /translation="MTEPPSPGASSPPPPPASPPAPPPSQADLSRERIVAAATELFAEH
                     GYDGTTTRTIAAAAGLNVATVAYHVGGKADLYREVMRRAHEAERAAVAAALGEFAGRAG
                     ADPAGAAAGFADRYLDFCLERPDVPALWMRRWLADAAEFAGLEEEYARPLLESVRDALA
                     AALPGVPPERAELAVWTVLWTVHGFCRSMIRAQVPRFRVHLRGLVLRELGLGAGS"
ORIGIN
        1 gacggtgggc acgcccgcgt ccacggcttc gcgcagcagc gcggtgaccg actcgacgtt
       61 gacggcgtgc atccggccgt ggtcggcgcc gggctggtag tactcgcgga agtacgcggc
      121 ggtgtgcacg acggcgtcgg cgccgcgcag ggcgtggcgg taggcggccg ggtcggtgat
      181 gtcggcggtc gcgacggtga tccgggtgtc ggccgggagc agccgggcag ccttctccgg
      241 gtcgcggacc agcgcggtca cctggtggcc cgcggcgagc agggcgcgga ccacggcgcc
      301 gccgagcagg ccggtggcgc cggtgaccac cacccggtcg ggtgcggggc ggacgtcgtt
      361 catcgggtct ccttccccgc ggcggcgggt gtggcggcgg gcgcgggctc ggcgggcgcg
      421 gggtctgcgg cgggcgcggg cccggcgggc gccgggcggg cctcctcgcg caggccgaag
      481 cgctcctgga agcgccgcag cggggcgggc gcccaccagg tggcgcggcc gccgagcgcc
      541 atcacggcgg gaacgaggac gcagcgcacc acggtggcgt cgaccaggac ggcgagcgcg
      601 acgccgaggc cgagcatctt ggtgttggtg atccgggagg tcccgatcgc gatcagcacc
      661 accgagagga tcaccgccgc ggcggtgatc agtccgccgg tgtgccggat gccggcggcg
      721 acggccccgt gcgggtcggc gccgcggtcg cgctcctcct tgacccgggc gagcaggaac
      781 accccgtagt ccatggagag gccgaaggcg acgcagaaca tcagcaccgg cagggtggtc
      841 tcgacgctgc cggtcggggt gaagccgagc accccggaca ggtggccctc ctggaagacc
      901 cagaccacca cgccgaacat cgcggtgagg ctgagcgcgt tcagcagcac ggcctgcagc
      961 gggatcagca gactgccggt gagcaggaac accagcacca gggtggcgac ggcgatcagc
     1021 gccccggcgg gcagcagccg cacggcgatg gcgtgcttgg cgtcgaccag gccggcggcc
     1081 tggccgccga ccgccggccg cagcgcgggg aagccctgcc cggcccggcg gatctcgccg
     1141 accaggtcct ggttggcctg ggagacgtcg ccgcgccggt cggcgggggt caccgtcagg
     1201 tggccgtacc cgtcggcggt gcggacgctg tcggcgggcc cggcggcgcc ggccggggcg
     1261 ccggcccggt aggggccgag cggggtggcg acctcggtga cgcccggcag cgcggagagc
     1321 ttcgccgcgt agtcggcgag cgcggcccgg tcgggcccgg tgacggtgcc ggtggcggtg
     1381 gcggtgacgc ggacgatcac gtcgacggcg ccggtggcgc cggccgtgaa ctcgtcccgg
     1441 acggcttgct ggacgacccg ggcctcggtc ccggccggca gctgccggtc gtcggcggcg
     1501 gcgaactgga cccgcaggaa cggggcgccg gcagccagca gcagggcggt gacggcgagc
     1561 gcgaacagcg gggcgcggcg catcaccgcg gtcgcgaccc ggtaccaacc ggcgccgggc
     1621 ccgccgtcgg gggcggccgg gcggcgcggg cggcggggca gcgggatgcg cagcgagtcg
     1681 atccggtggc cgagcagggt gagcagcgcg ggcagcacga tcagcgcggc cgcggcggcg
     1741 atcagcacca cgctgatgcc cgcgtaggcg aaggagcgca ggaagtacag cgggaagacc
     1801 agcatcgcgg agagcgagac ggcgacggtc agcgcggaga acagcaccgc ccggccggcg
     1861 gtccgcaggg tggtggcgac cgcctcgcgg acctccgagc cgcggtcgcg ctcctcgcgg
     1921 aagcggcgga ccacgagcag cgcgtagtcg atggccaggc cgaggccgag cgcggtggtg
     1981 aggttctgcg cgaacaccga cacctcggcg aaggaggtga tcacccgcag cgccgcgttg
     2041 gtgccgagga tcgcgcagat cccgacgacc agcggcagca gggcggccgt gaggccgcgg
     2101 aacaccagca ccaggatgag cagggtgacg ggcagcgcga tcagttcggc gcgggccagg
     2161 tcctcggcga tggtgccctg catctgctcg cggacggcga ccgtgccgcc gagcctgacg
     2221 tccagctcgg gcaggtccgc ggggctgccc cggtagtgcg gggcgatccg gtcgaagacg
     2281 gcccgggcgc ggtcctcgcc gccctcgatc cgggcgacga tcagcgcctt gccgtggtcg
     2341 gcggagcgca gttcgacggc gccggtctgc cagtaggagc cgacgccggc gatgccgggt
     2401 tcgaaggcca gccgttcgcc gagccgggcg ccggcccggg cggtggcggg gcggtcgacg
     2461 ctgccgccgt ccttggcggt gacctccagg accaggttgg gcgagccgcc ggggaaggac
     2521 tgttcgagca gggtcgcggc gtgcgccgac tcggaggacg ggtcggtggt gccgccgctg
     2581 tgcaggcggt cggtgacccc ggccccgacg accagcgcgt tgatcaggaa gaccagcgcg
     2641 aacagcagga cggtgcgggg ccggagggtg accgtccggc tcagtcggtg gagcatcggg
     2701 atcctcacgg ggcgggggcg gcggagcgct gcccggtcgg cagggcggac agcagcgcgt
     2761 ccaccccgtc cggccggtcg agggcgacgg caccggccac cacgccgtcc gggcgcagca
     2821 ggcaggccag cgcggggccg ggggtcggcg ggtggccggg cagggtgccg gccgcgatgt
     2881 cggggttcgc cgggcccgcg gggaggtccg cggcggtgcc gggcggcagc agcagcagga
     2941 cgtcggtgag gtcgggcagg ccgcgcaggg tgagttcggc ggtggcccgt tcggccgcgt
     3001 cggcggcggc cgggccgtcg cccgcgatca gcacggtgtg ccggccggtc gccaggtagt
     3061 cgtgcaggct ggtggccgag gtgccgcgga gcggccgcag cggggcgtcg ccgagccggc
     3121 ggccggggac gggggcgccg gggacggtgg tcggccggac ggccgggctg gccgtcaggt
     3181 cgaggtcgag ctgggcgagt tcggggacga tccggcgttc cagggcgccg gtgcgggcga
     3241 gcgtgccgag cagcaggtcg cgcagcgcgc gggccgggcg gggccggagc agccagagcc
     3301 tggtctgccg ttcggagttg cgcacggccc tggcggagac gggccggcgc tccgggtcgt
     3361 agctgtcgag cagggccggg tcggcgccgc ggagcaccgc ggcgagtttc cagccggcgt
     3421 cgaagccgtc ctggacgccg gtgttgaggc cctgcccgcc ggccgggctg tgcgcgtgcg
     3481 cggcgtcgcc ggccagcagg acggggccgt gccggaagcg ttcggcgacc ttggtgtgga
     3541 cccggaagcg gctggtccac caggtctccc ggacggtcca gcggccgtgg ccgcgggcgt
     3601 ccaggagctg ctggagctcg gcgtcgtcgg gcgggccggt gcgggtgccg ggcggcaggt
     3661 cgaagaagat ccggtgtccg ccgtcgggca gcgcgaccag caccaggacg ccgtccgggc
     3721 tgaggtggta ctgggcctcg tcgggcggca ggtcgccgtc gatccggccg tcgccgagca
     3781 cgaactcgcg gtcgtggctg ctgcccgggt acgggacgcc gatgctctcg cggacggtgc
     3841 tgcgggtgcc gtcggcgccg atcagccagg gcaccgacac ggtctcggca gtgccgtcgg
     3901 ccctggtcag ggccacctcg gcgcggccgt cggcggtggc gaccgcggtg acggcggtgc
     3961 cccactcgac ccggccgccg aggtcgagca gccgctcgta gagcagttcc tcggtgaccg
     4021 gctggggcac gcacagcggc cgggggaagc gggtgccgga caggccgccg aagcggacgc
     4081 cggccagtcg gcggccgccc gaccagtagg aggcaccgtt gagcggcagg gcgcgctcgg
     4141 ccagcgcgtc ggcgacgccg agccggtgca gcgcctccag ggcgccgctc cacatcacca
     4201 gggccttcgg ctcgggggtg aagcaggccc ggcggtcgac cagccggacc gggacgccgt
     4261 gctggagcag ggtgcaggcc gcggtcaggc cggtcggccc ggcgcccgcg accagtacct
     4321 cgggggccgc gctcaccgct cccccgcccc ggcggccagc agcgcgtcga cgtcccggac
     4381 ggcctgcgcg tcgtcctgga acagcacggc cttgaagccg agccgttcgg cggccacgca
     4441 gttctccgcg gtgtcgtcga ccagcaggca gcgttcggcg gtggtgccga gccggtccag
     4501 cagccgccgg tacagctccg ggtcgggctt gcggacccgc tccgcggagg agtcgacgac
     4561 cgcgtcgaac agctcgtcga ccgggagggt agcgcgccag cgcgggcccc actccacgac
     4621 gttgttggtg agcagcgcga tccggtggcc gtcggccctg agcttgcgcg cgtagtcgac
     4681 gagccggtcg ttggcccggc ggccgaggaa ccacagctcg ccgaacgggc ggccgccgag
     4741 gacggcctcc gagcccggcg ggagctcggc gagcagccgc tcggtgaact cccgctcggt
     4801 gatcgcggcg acctccagct cggccatcgg gctgatcccg tggcgctcgg tggcggcggc
     4861 gaacgccttg gcgatgtcgg cggtgccgat gccggtgcgg tggccgaacg cggcgaaggt
     4921 ctccggcagc gggttggtca ggacgccgcc gtagtcgaac accacggtgt cgacgggccc
     4981 gaccggcggg tgtccggggg tcagctccac tgggtcacct cgatcgagag ggtggcgacc
     5041 ggcaggccct cctgggacac cgtgatctcc accgggacgg tgacggcgcc gaggccgccg
     5101 tcgaagccgg cctcgccgac ccgggcgacc gccctggtga ccaggtcgag ttcggcgaag
     5161 tcggtgaacg cggcccgggc ggcggtgacc accaggctct ccgcgggcag cccgtgcagc
     5221 ttggcgaccg ccgcgacgga cagctgccgg gcggcctcca gctggaggtt gccgggcacg
     5281 tggtccaact ggtggtcgaa caggtgcggg tgcccggtgt cgaccaccag cagcgccgag
     5341 gcggtgccgg agtcgctgac cgagggctcg gtcagcaccg tgttggcctc gtcgcgccgg
     5401 ccgaccaggg cgggctcgga gcgcagcagc tgcgggtggc gcggcgagcg gccgtccagc
     5461 ttcttgcggt tcccggcccg gagcatctgg aaggcccggc tgctgacgaa ggtcagcgcg
     5521 ccggagccga cgatggcggg ctcgccgtcc agccagaggg tgccggtgaa gtcgaagccg
     5581 agcacccggc cgccgccgtt gcgctgcggg ctgacggtgg tggagagcac ggcgtgggcc
     5641 ggtcggcggc cgatccgcag cgcctccagg tcgaccacgt ccaggttgag ggcgctgaag
     5701 atgaaggccc ggtcgagcgg cacgtccagg tagcggtggg cgaccagcac gccggactgc
     5761 cgcagcacct cgaccagcag gaggaagtcg tagctgtcgc cgtgctcgcc gaccacgtgg
     5821 ccgcgcggca gttgggcggc cacctcgaag gtgtgccggt cgtcggcggt ctccgcctcg
     5881 gtgatccggg ccgagtcggt gacgaacacc tcggcgaccg cggcgcggtg taccagggcg
     5941 cgcgggaccg tcgcgtcgaa gcggaggtcg gcctgcggcc ggggccgttc ccgcccgtgc
     6001 tcctgcggat cgcgcggcag gacgtcgggt atcgcgttca tttctgttat cccccgtatg
     6061 acatctggaa tcgcgtgggc gggacaccgc cgtgtgcccg gggtccgctg cgttcccccg
     6121 ccgcgccccg cctcggccac cactgtgctg gtcggcgctt cccatcggct gacccgggcg
     6181 ggaagccgtc gggaagcggc cccgtgacgt ccccgtgacg gcggcggaaa agcgcggacc
     6241 cggccggaac gcggtggtag cgtcgcgccc ggtcggttgt ccacggatga caacgaggcg
     6301 ggggcgatgg cgggcgggac gtggttcgag gcgatcggac cggtgcgggt gcggcggggg
     6361 ccggcggaac tggacctcgg accacccaga cagcgggcgg tgctgaccgt cctgatcctc
     6421 aaccaccggc gcccggtgtc gacctcgcgc atcgtcgaac tggtctgggg cgccgacgcc
     6481 cccgaccgcg gcgtcaacct ggtgcagaag tacgtctcgg ggctgcgccg cgtcctccag
     6541 ggcgtcgccg aactctcctg gaccgaggcc gggtacgtgc tggtgccgga cggcacggtg
     6601 gacgtcgagc ggttcgacgc gctgctcacc gcgggccgca ccgcccgggc cggcggcgac
     6661 ccggccgccg cggcggcggc gctcgccgag gcggtggcgc tgtggcgcgg cccgctggcc
     6721 cagggcatgg gcgggcccgg catcgaaccg ctgcggctgc ggctcaccga atcctggctc
     6781 gccgccaagg aggacctgtt cgacgccgag ctggagctgg gccgactgcc cgaccggctc
     6841 cccgaactgc gccggctcac cgccgaacac ccgctgcgcg aacggttccg ggcccagctc
     6901 gtgctggccc tggcccaggc cggccggccc gccgaggcgc tcgccgagca cgaccgggcc
     6961 cggcgccggc tcgccgacga gcacggcctc gaccccggcc cgcacctgcg ggccgcccag
     7021 gagcaggtcc tgcggatggc ctcggaccgg gcggccccgg ccgaccccgc caccccgccc
     7081 gccctctcct cccccgccgc cctctcctcc cccgccgccc tgccaccagc ccaactcccg
     7141 cccgctccaa gggtgttcgt cggccgcacg gcactcaccg cccggctcgc cgaactcctg
     7201 gcgcccaccg ggccgccacc cctggtctgc ctggagggca ccgccggcgt cggcaagagc
     7261 gcgctggccc tgcgcgccgc ccaccgggcc gccgggctct tcccggacgg gcagctcttc
     7321 gccgacctgc gcggcttcgg ccgcggcctg cccgccgacc cgtccgacgt gctcggcggc
     7381 ttcctgcgcg ccctcggggt gcccgccgaa cgcctgccgc aggacctcgg cgagcggatc
     7441 ggcctctacc ggtcgatcct ggccggccgg gccgtcctgg tggtgctcga cgacgcgcgg
     7501 gacgccgacc agatccggcc gctgctgccc agcggcaacc ggtgcgccgt cctggtgacc
     7561 ggccggcgcc gcatgatcag cctgaccgtg cgggagggcg cgcaccggct gatcgtcccg
     7621 gtgctgaccg ccgaggagtc ccgcggactg gtcaactcgc tgctcggccc cgggcagacc
     7681 cgggacaccg cggcggtcga ccggatcatc gcgctctgct ccggactccc gctcgccctg
     7741 cggatcgtca ccgccaacgc cgcccaccgg gccgacctcc cgctcgcccg gatcgcccgc
     7801 gaactggccc gcgaccgggt ggccgcgctc tccgtcccgg acgaggcgca gaccgcgctg
     7861 gactgctcga tcggcctctc ctacgacctg ctcccgcccg gccaccggga actcttctgc
     7921 ctgctcggac tcgtccccgg accggacttc accctccggg ccgccaccgc gatggccggc
     7981 gccggagccg ccgcgaccga acgcgggatg cgcgacctgg aagtcgccaa cctgctggaa
     8041 cgcaccgccg accgctaccg cttcccgcac gagctgctcc agctgttcgc ccaggaacgc
     8101 gcccaccggg aggtgcccgc cgcccggcgg gacgaggccc tgcaccggct gctgggccac
     8161 tacctggccg ccgtgctccg cgccgagggg cgcgaccgca gggccgccgg cgcggacctc
     8221 ccggcgctcc cggcctcctc ggagccctcc gacccctcgg acccctcgga cctctccgac
     8281 ctctccgacc tctccgaccc cgacttcccg gacgccgacc tcgaagagga cgacctggaa
     8341 agcgagttcg acaacctgat ggcgaccgtc ctgcacgccg ccaccgccgg accgtaccca
     8401 cccgtctggc aactcgccga cgcactgcgg ccgttctgca agcagcacgc ccgcaccgcg
     8461 cagtggtccc ggctggccgg ggccggactg gccgccgccc ggcgggccgg cgaccggcgg
     8521 gccgaggccg ccatgctgct caacctcagc gacgccgagc acaactccgg cctggtggag
     8581 gagggcgccg aacacgcccg gcaggcgctg gcgctcggcc gcggactcgg cctcggccgg
     8641 atcgaggcgt ccgcgctgga ccagctcggc cgggccgcct ggatccgcgg cgccctggag
     8701 accgcccgga cgctgctgac cgccgccgtc gacgcgcaca cccgcaccgg cgaccgactc
     8761 ggcctggccg tctcgctgtc ggcgctgggc cggaccgagt tcgacgcggg ccacaccgaa
     8821 caggccgccc tgcactgctc ccaggtgctg cggctggccc ggaccatcgg cgcggccgcc
     8881 gtcgagacca tggccctggt cgagatgggc gtcctgcacg ccgccctggg tcggccggag
     8941 aacgccgccc gctccctgcg cgccgccctc gcgctcgccc gcgacggcgg ccaccgccgc
     9001 gccgaggcgc tcgccctcgc ccggctcagc ctgctgcggg ccggcggcga ccggccgcgg
     9061 gaggccctgg ccgaggcccg caacgcgctg cacgtcgcgg aggcgcacgg cgaccgctgg
     9121 atcgaggccg agtgcctgaa cacgacggcc cgcaccgcgc tggccaccgg cgacaccgac
     9181 accgccgccg cccgccaccg ggccgcgctg accctggcca cccgcctcgg ctaccgccgg
     9241 gccgaactcc aggccctgct cggcctcgcc gagaccgccc tgcgccgggg cgacacccag
     9301 cgcgccgcgg actacgcggg ccgggcccgc gccgccgccg ccggcctcgg gcacctcccg
     9361 ctggccgccg aagccctccg catcgagggc cgcctccgcg ccaccccgga cgctgctccc
     9421 gaccccgagc ccgccaccgc gcccgagccc gcgtccgccg accgggcgca gctgggctgc
     9481 tgacgacgcc cgggaagtgg cgggcgcccc ggccgcgttc cttcggctgc ggatttaatg
     9541 cacccgtcat ccatcaaacg gttgattgac cgaggtgccg cgcgtacgct ccccgccatg
     9601 accgagccac cgtccccggg cgcgtcgtca cccccgcccc cgcccgcgtc cccgcccgcg
     9661 ccgccgccca gccaggccga cctgagccgg gagcggatcg tcgcggccgc caccgagctg
     9721 ttcgcggagc acggctacga cggcaccacc acccggacga tcgcggccgc cgccgggctg
     9781 aacgtcgcga ccgtggcgta ccacgtgggc ggcaaggccg acttgtaccg cgaggtgatg
     9841 cggcgggcgc acgaggccga gcgggcggcg gtggcggcgg cgctgggcga gttcgcgggg
     9901 cgggccgggg cggatccggc gggggcggcg gcggggttcg ccgaccggta cctggacttc
     9961 tgcctggagc ggccggacgt cccggcgctg tggatgcggc gctggctggc ggacgccgcc
    10021 gagttcgccg gcctggagga ggagtacgcc cggccgctgc tggagtcggt gcgcgacgcg
    10081 ctggccgccg cgctgcccgg ggtgccgccg gagcgggccg aactggcggt ctggacggtg
    10141 ctgtggacgg tgcacggctt ctgccgctcg atgatccgcg cgcaggtccc ccggttccgc
    10201 gtccacctgc gcggcctggt gctgcgcgag ctgggtctgg gggcggggtc gtgagcgcgg
    10261 cggcggacgg cgcgacaggc cgccggcagc tcgccgggta cgcgctgggc tcggtcggga
    10321 cgggcatcca cgcgaccgtc cccggcctgc tgctgctgta cttcatgacc gacgcgctgg
    10381 gcgtgccggc cggggtcgcg ggcctggtgg tggcgctgcc caaggcgtgg gacgcgctgt
    10441 tcaacccgac cgtcggcgcc gccagcgacc gggaggcgct gcgcaccggc cgccggaccc
    10501 ggatcctgct ggcgggggcg ctggcgctgc cagcggcgtt cgccgcgatg ttcctctccc
    10561 cgctgaccgg ctccggcgcg gccgcctggg tggcggtgac cttcgtgctg gcgtcgagcg
    10621 ccttcgcgct gttccaggtg ccgtacgtgg cgctgcccgc cgagatgtcg gaccggcccg
    10681 aggtccgcac ccggatcatg gtgtggcgga tcgtctgcct gaccgtcggc atcctggtcg
    10741 cgggcggcct ggccccggcc gtggtgtcgc tggccgggga cgggcggcgc ggctacgcgg
    10801 tgatgggcgc ggtggtcgcc gtcctgctgc tggtgacgct gctggtgccc gcgctgggct
    10861 cgcgccgggt gcccgcccgg ccggggccgg agccgctcgg gctgctcgcg gcgctgcgca
    10921 cggcgcgcgg caaccgggcg ttcttcgcgc tgctgggcgg gttcgtgctg caggcggcgg
    10981 cggtggcgat catgctggcg gccgcgccgt acgccgcgac gtactggctg ggcggctacg
    11041 g
//
