LOCUS       NC_016109              10801 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Kitasatospora setae KM-6054, complete sequence.
ACCESSION   NC_016109
VERSION     NC_016109.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora setae KM-6054
  ORGANISM  Kitasatospora setae KM-6054
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1
  AUTHORS   Ichikawa,N., Oguchi,A., Ikeda,H., Ishikawa,J., Kitani,S.,
            Watanabe,Y., Nakamura,S., Katano,Y., Kishi,E., Sasagawa,M.,
            Ankai,A., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Nihira,T., Horinouchi,S., Ohnishi,Y., Hayakawa,M.,
            Kuzuyama,T., Arisawa,A., Nomoto,F., Miura,H., Takahashi,Y. and
            Fujita,N.
  TITLE     Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary
            snapshot of the family Streptomycetaceae
  JOURNAL   DNA Res 17 (6), 393-406 (2010)
   PUBMED   21059706
  REMARK    DOI:10.1093/dnares/dsq026
REFERENCE   2  (bases 1 to 8783278)
  AUTHORS   Ichikawa,N., Watanabe,Y., Nakamura,S., Katano,Y., Oguchi,A.,
            Ankai,A., Yashiro,I., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Ikeda,H., Ishikawa,J., Horinouchi,S., Ohnishi,Y.,
            Hayakawa,M., Nihira,T., Kitani,S., Kuzuyama,T., Arisawa,A.,
            Nomoto,F., Miura,H., Takahashi,Y. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2009) Contact:Director-General Department of
            Biotechnology National Institute of Technology and Evaluation, NITE
            Genome Analysis Center (NGAC), Department of Biotechnology; 2-49-10
            Nishihara, Shibuya-ku, Tokyo 151-0066, Japan URL
            :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000269985.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:32:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,713
            CDSs (total)                      :: 7,605
            Genes (coding)                    :: 7,487
            CDSs (with protein)               :: 7,487
            Genes (RNA)                       :: 108
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 25 of 118
            Pseudo Genes (incomplete)         :: 95 of 118
            Pseudo Genes (internal stop)      :: 9 of 118
            Pseudo Genes (multiple problems)  :: 11 of 118
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:33:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2127750
            Orig. end                         :: 2138551
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010968.1.
            'Otoguro, M. is at NITE Biological Resource Center (NBRC). Ikeda,
            H., Miura, H. and Takahashi Y. are at Kitasato Institute for Life
            Science. Ishikawa, J. is at National Institute of Infectious
            Diseases. Horinouchi, S., Ohnishi, Y. and Kuzuyama, T. are at The
            University of Tokyo. Hayakawa, M. is at University of Yamanashi.
            Nihira, T. and Kitani, S. are at Osaka University. Arisawa A. is at
            Mercian Corp. Nomoto F. is at Nagase & Co. Ltd. The other authors
            are at NITE Genome Analysis Center (NGAC).
            Please visit our web site. URL:http://www.bio.nite.go.jp/
            DOGAN ; Database of Genomes Analyzed at NITE. The database contains
            genome sequence data, physical maps and the genomic and proteomic
            data of the microorganisms analyzed.
            URL:http://www.bio.nite.go.jp/dogan/Top
            The microbial strain and microbial genomic DNA clones used for the
            sequencing project are available through the NBRC.
            URL:http://www.nbrc.nite.go.jp/e/index.html.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5801)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(416..429)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            646..1032
                     /locus_tag="KSE_RS09415"
                     /old_locus_tag="KSE_19170"
     CDS             646..1032
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135059.1"
                     /locus_tag="KSE_RS09415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19170"
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="WP_014135059.1"
                     /transl_table=11
                     /translation="MADTALDRLAAGNYLLITSYKKDGSAVPTPVWVVRDGDTLGAWTA
                     AGSWKVKRIRRRGDVLVGPCDVRGNPSGKSLAAHAEIADAATTERYRQLLARKYGLLGR
                     LTLLGSRLRRGRAGTVGITIRLDG"
     gene            complement(1050..1835)
                     /locus_tag="KSE_RS09420"
                     /old_locus_tag="KSE_19180"
     CDS             complement(1050..1835)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135060.1"
                     /locus_tag="KSE_RS09420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19180"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_014135060.1"
                     /transl_table=11
                     /translation="MRQYRTMVAVGDSFTEGMCDDVLPDGHYRGWADRVAAALAAEAAP
                     DGFRYANLAVRGKLIGQIRAEQVDLAAATDADLVTLAGGLNDVLRPGCDVAEVERLLGE
                     CVRALLAEGRTVVLFTSTDPTRRMAGSRRLLPAILRMKAYVESLVRDEPDRVVLVDLFS
                     APGFDDRRLWAEDRLHLSPEGHRRVALAVLEALGRPAAEDWRAPLADPDPRTRARKARD
                     DARWLGTHVGPWIGRRLRGRSSGDGRPAKRPELLPYDGS"
     gene            complement(1921..3171)
                     /locus_tag="KSE_RS09425"
                     /old_locus_tag="KSE_19190"
     CDS             complement(1921..3171)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135061.1"
                     /locus_tag="KSE_RS09425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19190"
                     /product="cytochrome P450"
                     /protein_id="WP_014135061.1"
                     /sec_met_domain="p450 (E-value: 8.1e-18, bitscore: 63.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTVPSEPFRGCPVLDPAAAALHVEAAGLRARGPAVRVELPGGVL
                     AWAVTRHAVIQALTTDPRVSRDYRRHWPGAGDVPPGWVLGSVAFQDSLVNHYGDAHREA
                     RGRIAPTFLPRRVERMRPQVQATADRLVDALAALPPGERADLRHALARPLTMTVICDLF
                     GVPAHLRDPLGELIDTVLDSSLDEADSLAALTRLYERLAELLAYKRAHPASDLTSDLLL
                     SDRPGARPLADHELLGTLLTMIAAGFETAVNLIVSAALELLDHPEQLAAVTAGQTGWSD
                     VIEETLRLEGPVMYLPLRYALADLDLGEGVLIPKGDPIIIGFAAAGRDPERHPDRPDEF
                     DPARADKGHLAFGHGPHFCVGAPLARLEAEIALRTLFERLPELAAAEPGRRPARLPSLI
                     VNGPAVLEVVPRPATAR"
     gene            complement(3168..3461)
                     /locus_tag="KSE_RS09430"
                     /old_locus_tag="KSE_19200"
     CDS             complement(3168..3461)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000101: Heterocyclization (61% identity)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.5"
                     /locus_tag="KSE_RS09430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19200"
                     /product="cytochrome P450"
                     /protein_id="WP_014135062.1"
                     /sec_met_domain="p450 (E-value: 1.1e-07, bitscore: 29.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPDAPAHPDRPDVFDPARADTSHLAFGYGPHFCVGAPLARLEAE
                     IALRTLFDRLPEPAAADPGRPPARIPSTVVNGPAALPVVPRPVVRDPKEAHA"
     gene            complement(3458..4696)
                     /locus_tag="KSE_RS09435"
                     /old_locus_tag="KSE_19210"
     CDS             complement(3458..4696)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135063.1"
                     /locus_tag="KSE_RS09435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19210"
                     /product="cytochrome P450"
                     /protein_id="WP_014135063.1"
                     /sec_met_domain="p450 (E-value: 1e-15, bitscore: 56.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSSVPRCPFLDPAGSALHAEAAGLRARGPAVLVELPGGVRAWS
                     VTRHAVIHALTTDPRVSRDFRLHWPAAAEVPQGWVLGPVAFQDSFVNQYGAEHRASRGR
                     LAPTFLPRRVERMRAQVQATADGLVAALGALAPGERADLRSTLARPLTLTVICDLFGVS
                     EEMRAALCAVIDTVLDSSLGEERALAAQIELNERLSALLAHKRARPAADLTSDLLLSDR
                     PGERPLSEQELIGTLFTMITGGFETAVNLISSAALELLADPAHLAALRAGAIGWADVIE
                     ETLRFEGPVMHVPLRYAVEEIDLGEGVLIAKGDPIIIGFAAAGRDPEAHPDRPDVFDPA
                     RADTSHLAFGYGPHFCVGAPLARLETEIALRTLFERLPDLALAEPGRAPERVPSMIING
                     PAALDVVPRPVSR"
     gene            complement(5001..5801)
                     /locus_tag="KSE_RS09440"
                     /old_locus_tag="KSE_19220"
     CDS             complement(5001..5801)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894062.1"
                     /locus_tag="KSE_RS09440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19220"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_014135064.1"
                     /sec_met_domain="Linocin_M18 (E-value: 7.7e-93, bitscore:
                     309.1, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRDLAPVSAAAWAEIEEEARRTFRLHVAGRRVVDLSGPDGP
                     ELAAVGTGHLADIAAPGSGVQARAREALQVVELRVPFTVTRAAVDDVERGSKDSDWDPV
                     KDAARTIALAEDRALFDGYQAAGITGIRDGSTNHPIALPADVREYPNAVSRALTALRLA
                     GVGGPYSLLLGADTFTAVNETSDHGYPVHNHLARLLDGEIIWAPAVEGAVLLSARGGDF
                     ELHLGEDLSIGYLSHDDTTVHLYLQESLTFQLHTPEAAVALTSS"
     gene            complement(5798..6853)
                     /locus_tag="KSE_RS09445"
                     /old_locus_tag="KSE_19230"
     CDS             complement(5798..6853)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135065.1"
                     /locus_tag="KSE_RS09445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19230"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_014135065.1"
                     /transl_table=11
                     /translation="MPQPQPVLATLTGAAVFLVLAIEDGGEETVRDLLPDLGGLARTVG
                     TRAPHERVSAVLGIGSDAWDRLFAGPRPARLHPFVELAGPRHLAPATPGDLLLHVRAER
                     MDLCFELATLVSRRLGDRVTVLDETHGFRYFDCRDLIGFVDGTENPVGPDAADAVLIGD
                     EDPAFAGGSYVIVQKYLHDMAGWNAIGTEQQERVIGRTKLDNVELDDAAKPADSHVALN
                     TVTGPDGEDRDIWRLNMPFGSFAEGGECGTYFIGYARDPGVTEEMLRNMFLGRGPAAHD
                     RLLDFSTPVTGTFFHVPSADFLNDPPPAPGAPAPPADTPPADTPSADAVPAPPVRDGSL
                     GIGSLKRSPAP"
     gene            complement(6970..7428)
                     /locus_tag="KSE_RS09450"
                     /old_locus_tag="KSE_19240"
     CDS             complement(6970..7428)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135066.1"
                     /locus_tag="KSE_RS09450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19240"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_231873151.1"
                     /transl_table=11
                     /translation="MEQREFGRLLQGLASEMNQLGHDFAAASGLHATDVQALLAVLRAP
                     DPPGLTPGGLRAQLGLTSGAVTAVLDRLERAGHVHRTRDEHDRRQVHVHYHPDAARIAG
                     EWFAPVADRTDRVRAEFTPEQVATVVRFLTRMTEELAGLREHRRAEPS"
     gene            complement(7508..8533)
                     /locus_tag="KSE_RS09455"
                     /old_locus_tag="KSE_19250"
     CDS             complement(7508..8533)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135067.1"
                     /locus_tag="KSE_RS09455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_19250"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_014135067.1"
                     /transl_table=11
                     /translation="MPVEPPEAGASTGEVARRLGVSPTTVRSWERRYGIGPAERAAGRH
                     RRWTARDVAVLEEMCRLTASGLPPAEAARLALGPAVLLPAPTDPAPPAAPASPPAAQPL
                     VPQPLVPSPAVPAPAGEVRPEVRGLARAARRLDSAEVAGLLRTGVERLGAVAAWTDVMA
                     PALRAAGRRWAADGGERYVEVEHLLSWHVSSALRRVAEPPVDAGGSPVLLAAMPGEQHT
                     LALEAVAAGLAERGLPFRIFGAALPAGALAEAVRRTGPAAVLLWSQTRATADPAPARAV
                     AATAWGTRGARVRPVLLAAGPGWQRATRPPGALAPRDLPGALDLLARAAGRGGEGAGRA
                     R"
     misc_feature    complement(8606..8620)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.1"
                     /tool="antismash"
     misc_feature    8607..8621
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: medium, score: 24.77"
                     /tool="antismash"
     misc_feature    complement(8668..8682)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 13.75"
                     /tool="antismash"
     misc_feature    complement(9381..9392)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: weak, score: 17.94"
                     /tool="antismash"
     misc_feature    9841..9855
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.56"
                     /tool="antismash"
ORIGIN
        1 cgcccagcgc ggccggggcg tggagatcga gtcgctgggc ggcagcgtgc tccaggtggc
       61 ccggcagttc ggctggtccg gccgggtcga gcagcactac ggctcgttcg agttcctcgg
      121 ccggtacgtg caccggatct cgcagctctc gctgctgctg accgtcgccg cgttcggctg
      181 gctgctgctc tggcgggtcc gggcccggcg ctggacggcc gccaccccgt acgacgcggc
      241 gctgaccgcg gtgctgctgt tcaccgtcac cagccgggtg atctccccgc agtacctggt
      301 ctggctgatc ggcctgggcg cggtctgcct gaccgtggag tgcaccaccc agcggccggt
      361 ggcgttcctg ctgctgcccg ccgtcggcgt caccaccgtc gactacccgc tgttcttcga
      421 ctcgatgcag gccggcggct ggtggccgac cgccgtgatc ctgctgcgca acgggctgct
      481 gctggccgcc tcgctctggt cggcggcccg gctctggcgg gccaccgtct cgcccgcgcc
      541 cgaaccccgc ccggccgacc gcgcgcacgg cgacccgcgc cccgacccgg acccggaccc
      601 cgtccccgcc gcgtgacgcg gggtcagcgg ccgtacactc ccccaatggc cgacaccgcg
      661 ctcgaccggc tcgccgccgg caactacctg ctgatcacca gctacaagaa ggacggcagc
      721 gccgtcccca ccccggtctg ggtggtccgg gacggcgaca ccctcggcgc gtggaccgcc
      781 gccggctcct ggaaggtcaa gcggatccgg cggcgcggcg acgtcctggt cggcccgtgc
      841 gacgtccgcg gcaacccgag cggcaagtcg ctggccgcgc acgccgagat cgcggacgcc
      901 gccaccaccg agcgctaccg gcagctgctg gcccgcaagt acggcctgct cggccggctc
      961 accctgctcg gcagccggct gcgtcgcggc agggccggca cggtcggcat cacgatccgg
     1021 ctggacggct agccgctgcc cgggcgctgt cagctgccgt cgtagggcag cagttcgggc
     1081 cgcttggccg ggcggccgtc gccggaggag cggccgcgca ggcggcggcc gatccagggg
     1141 ccgacgtggg tgccgagcca gcgggcgtcg tcgcgggcct tccgggcgcg ggtgcggggg
     1201 tcggggtcgg cgagcggggc gcgccagtcc tcggcggcgg ggcggccgag ggcctccagg
     1261 acggcgaggg cgacccggcg gtggccctcc ggggagaggt gcaggcggtc ctcggcccag
     1321 agccggcggt cgtcgaagcc gggcgcggag aacaggtcga ccagcacgac ccggtcgggt
     1381 tcgtcccgca ccaggctctc gacgtacgcc ttcatccgga ggatcgcggg cagcaggcgg
     1441 cggctgccgg ccatccgccg ggtcgggtcg gtggaggtga acagcaccac ggtgcggccc
     1501 tcggcgagca gcgcgcggac gcactcgccg agcagccgtt cgacctcggc gacgtcgcag
     1561 ccggggcgca gcacgtcgtt caggccgccc gcgagggtga ccaggtcggc gtcggtggcg
     1621 gcggccaggt cgacctgttc ggcccggatc tggccgatca gcttgccgcg gacggccagg
     1681 ttggcgtagc ggaagccgtc cggggcggcc tcggcggcga gcgcggcggc gacccggtcc
     1741 gcccagccgc ggtagtgccc gtcggggagg acgtcgtcgc acatcccctc ggtgaacgag
     1801 tcgcccaccg ccaccatcgt ccggtactgc cgcactccgc ctcctgggtc cgtcgctcgc
     1861 tgccgcccca ccctaccgag gcgtaacgac gcgttcgggc ggcggtgcca gggggcggcg
     1921 tcagcgggcg gtggccgggc ggggcaccac ctccagcacg gcggggccgt tgacgatcag
     1981 cgaggggagg cgggccgggc ggcggccggg ttcggcggcg gccagctcgg gcagccgctc
     2041 gaacagggtg cgcagcgcga tctcggcctc cagccgggcg agcggcgcgc cgacgcagaa
     2101 gtgcgggccg tggccgaagg cgaggtggcc cttgtcggcg cgggcggggt cgaactcgtc
     2161 ggggcggtcg gggtggcgtt ccgggtcccg gccggcggcg gcgaagccga tgatgatcgg
     2221 gtcgcccttg gggatcagca cgccctcgcc gaggtcgagg tcggccagcg cgtagcgcag
     2281 cggcaggtac atcaccgggc cctccagccg gagggtctcc tcgatgacgt ccgaccagcc
     2341 ggtctggccg gccgtgaccg ccgccaactg ctcggggtgg tcgaggagtt cgagggcggc
     2401 gctgacgatc aggttgacgg cggtctcgaa gccggccgcg atcatggtga gcagggtgcc
     2461 gagcagttcg tggtcggcca gcgggcgtgc gccggggcgg tcggagagca gcaggtcgct
     2521 ggtcaggtcg gacgccgggt gggcccgctt gtaggcgagc agttcggcga ggcgctcgta
     2581 cagccgggtc agcgcggcga gcgagtcggc ctcgtcgagc gaggagtcga ggacggtgtc
     2641 gatgagttcg ccgagcgggt cgcgcaggtg cgcggggacg ccgaacaggt cgcagatcac
     2701 cgtcatggtc agcggccggg ccagcgcgtg ccgcaggtcg gctcgctcgc cgggcgggag
     2761 ggcggcgagc gcgtcgacca gccggtcggc ggtggcctgc acctgcgggc gcatccgttc
     2821 gacccggcgc ggcaggaagg tcggggcgat ccggccgcgg gcctcgcggt gcgcgtcgcc
     2881 gtagtggttg accaggctgt cctggaaggc gaccgacccg agcacccagc cgggcgggac
     2941 gtccccggct cccggccagt gccggcggta gtcgcgggag acccgcgggt cggtggtcag
     3001 cgcctggatc accgcgtgcc gggtcaccgc ccaggcgagc accccgccgg gcagctccac
     3061 ccgcaccgcg ggtccccggg cgcgcagccc ggcggcctcg acgtgcagtg cggccgcggc
     3121 cggatccagc accggacagc cccggaaggg ctcggacggg acggtgttca cgcgtgcgcc
     3181 tccttgggat cccgcaccac cgggcggggg acgacgggca gggcggccgg gccgttgacc
     3241 accgtggacg ggatccgggc gggcgggcgg ccggggtcgg cggcggccgg ctcgggcagc
     3301 cggtcgaaca gggtgcgcag cgcgatctcg gcctccagcc gggccagtgg cgcgccgacg
     3361 cagaagtgcg ggccgtagcc gaacgcgagg tgcgacgtgt cggcgcgggc cgggtcgaag
     3421 acgtccggcc ggtcggggtg ggcgggcgcg tccgggctca tcggctcacc ggccggggca
     3481 ccacgtccag ggccgccggg ccgttgatga tcatcgacgg gacccgctcg ggcgcccggc
     3541 ccggctcggc cagcgcgagg tcgggcagcc gctcgaacag ggtgcgcagc gcgatctcgg
     3601 tctccagccg ggcgagcggc gcgccgacgc agaagtgcgg gccgtagccg aacgccaggt
     3661 gcgacgtgtc ggcgcgggcc gggtcgaaga cgtccggccg gtcggggtgc gcctccgggt
     3721 cccggccggc ggcggcgaag ccgatgatga tcgggtcgcc cttcgcgatc agcacgccct
     3781 cgccgaggtc gatctcctcg acggcgtacc gcagcgggac gtgcatcacc gggccctcga
     3841 accgcagcgt ctcctcgatc acgtccgccc agccgatcgc gccggcccgc agcgcggcca
     3901 ggtgcgcggg gtcggcgagc agttccagcg cggcgctgct gatcaggttg acggcggtct
     3961 cgaagccgcc ggtgatcatc gtgaacaggg tgccgatcag ctcctgctcg gacagcggcc
     4021 gctcgcccgg acggtcggag agcagcaggt cgctggtcag gtcggccgcc gggcgggccc
     4081 gcttgtgcgc cagcagcgcg ctcagccgct cgttcagctc gatctgggcg gccagcgcgc
     4141 gctcctcgcc gagcgaggag tccagcacgg tgtcgatcac cgcgcagagc gcggcccgca
     4201 tctcctccga aacgccgaac aggtcgcaga tcaccgtcag cgtcagcggc cgggccagcg
     4261 tgctccgcag gtcggcgcgc tcgcccgggg cgagcgcgcc gagcgccgcc accagcccgt
     4321 ccgccgtcgc ctgcacctgg gcccgcatcc gctccacccg gcgcggcagg aaagtcgggg
     4381 cgagccgccc gcgcgacgcg cggtgctcgg ccccgtactg gttgacgaag ctgtcctgga
     4441 acgcgaccgg ccccagcacc cacccctgcg gcacctccgc ggccgccggc cagtgcagcc
     4501 ggaagtcccg cgacacccgc gggtcggtgg tcagcgcgtg gatcaccgcg tgccgggtca
     4561 ccgaccaggc ccgcacgccc cccggcaact ccaccagcac cgcgggcccg cgcgcccgca
     4621 gccccgcggc ctccgcgtgc agcgcgctgc ccgccggatc gaggaacgga catctgggaa
     4681 cggaagaatc ggacacggtg tgctcccctc accggaaatc cccgcacgtc cccccatggt
     4741 ggggcccgca ccgcaccccc ggaagcagct gttcgacttt ggcccgaatc cgctcccgcc
     4801 acccccaact cccttatctc cagccacagt tgaggacagc agacccagca ggagccacga
     4861 ccgggcgacg gcggactccg cgcgcccccg acgcaccgcg cgcccgggcg ccgtccggcg
     4921 cgggagcttc ccccgcgggc acggagaacc ccgcccgctc cgggcggccg gagcgggcgg
     4981 gggaaggggg tcggggccgg tcaggaggag gtgagggcga ccgcggcttc cggggtgtgg
     5041 agctggaagg tgagggattc ctggaggtag aggtggacgg tggtgtcgtc gtgggagagg
     5101 tagccgatcg agaggtcctc gccgaggtgg agctcgaagt cgccgccgcg ggcggagagc
     5161 agcacggcgc cctcgacggc gggggcccag atgatctcgc cgtcgaggag gcgggcgagg
     5221 tggttgtgga cggggtagcc gtggtcggag gtctcgttga cggcggtgaa ggtgtcggcg
     5281 ccgagcagca gcgagtaggg gccgccgacg ccggccaggc gcagcgcggt gagggcgcgg
     5341 ctgacggcgt tggggtactc gcggacgtcg gcgggcagcg cgatcgggtg gttggtggag
     5401 ccgtcgcgga tgccggtgat gccggccgcc tggtagccgt cgaagagggc gcggtcctcg
     5461 gcgagggcga tggtgcgggc ggcgtccttg accgggtccc agtcggagtc cttggagccg
     5521 cgttcgacgt cgtcgacggc ggcccgggtg acggtgaagg gcacgcgcag ttcgaccacc
     5581 tggagggcct cccgggcccg ggcctggacg ccgctgccgg gggcggcgat gtcggcgagg
     5641 tggccggtgc cgacggcggc gagctcgggc ccgtccgggc cggagaggtc gacgacgcgg
     5701 cggccggcca cgtgcagccg gaaggtgcgc cgggcctcct cctcgatctc ggcccaggcg
     5761 gcggcggaga cgggcgccag gtcgcggtgc aggttgttca cggtgcgggg ctccttttca
     5821 ggctgccgat gccgaggctg ccgtcccgga ccggcggggc gggcacggcg tcggcggacg
     5881 gggtgtcggc gggcggggtg tcggcgggcg gggcgggtgc gccgggggcg ggtggcggat
     5941 cgttcaggaa gtccgcgctg gggacgtgga agaaggtgcc ggtgaccggg gtggagaagt
     6001 ccagcagccg gtcgtgcgcg gcggggccgc ggccgaggaa catgttgcgg agcatctcct
     6061 cggtgacccc cgggtcgcgg gcgtagccga tgaagtaggt gccgcactcg ccgccttcgg
     6121 cgaacgaccc gaacggcatg ttcagccgcc agatgtcgcg gtcctcgccg tccggcccgg
     6181 tgacggtgtt cagcgcgacg tgcgagtcgg cgggcttcgc ggcgtcgtcg agttcgacgt
     6241 tgtcgagctt ggtgcggccg atcacccgct cctgctgctc ggtgccgatc gcgttccaac
     6301 cggccatgtc gtgcaggtac ttctgcacga tgacgtagct gccgccggcg aaggccgggt
     6361 cctcgtcgcc gatcaggacg gcgtcggcgg cgtccggccc gaccgggttc tcggtgccgt
     6421 cgacgaagcc gatcaggtcg cggcagtcga agtagcggaa gccgtgcgtc tcgtccagca
     6481 cggtgacccg gtcgcccagc cggcggctga ccagcgtcgc caactcgaag cacaggtcca
     6541 tccgttcggc ccggacgtgc agcagcaggt cgccgggggt cgcgggggcg aggtgccggg
     6601 ggccggccag ctcgacgaag gggtgcagcc gggccgggcg ggggccggcg aacaggcggt
     6661 cccaggcgtc cgacccgatg ccgagcaccg cgctgacccg ctcgtgcggc gcccgggtgc
     6721 cgaccgtgcg ggccagcccg ccgaggtcgg gcagcaggtc gcggacggtc tcctcgccgc
     6781 cgtcctcgat cgcgagcacc aggaacacgg cggccccggt cagcgtggcg aggaccggtt
     6841 ggggctgcgg catggcgggt gctcccaggg ggctgccgga cattcggacg cccgccgacc
     6901 atacccggca aaaggttcaa taccctgcga tgacgcccga atcgggcgtg tcaggcgcgg
     6961 ccggtcgact caggacggct cggcgcggcg gtgctcgcgg aggccggcca gctcctcggt
     7021 catccgggtg aggaagcgga ccacggtggc gacctgctcc ggggtgaact cggcccggac
     7081 ccggtcggtg cggtcggcga cgggcgcgaa ccactcgccg gcgatccggg ccgcgtccgg
     7141 gtggtagtgc acgtggacct ggcggcggtc gtgctcgtcg cgggtgcggt ggacgtggcc
     7201 ggcccgctcc agccggtcca ggacggcggt caccgcgccg gaggtcaggc cgagttgggc
     7261 gcgcagcccg cccggggtga ggccgggcgg gtcgggcgcc cggagcaccg cgagcagggc
     7321 ctggacgtcg gtggcgtgca ggccggaggc ggcggcgaag tcgtgcccga gctggttcat
     7381 ctcggacgcc agcccctgca gcagccgccc gaactcacgc tgctccaccg gccgccgtcc
     7441 cgccggctcc tgctccacca cgcccgtccg cccgcctgcc cggtcacccg ccgggccggt
     7501 cacgacctta gcgggcccgg cccgcgccct cacccccgcg acctgccgcc cgcgccaaca
     7561 ggtcgagcgc gcccggcagg tcgcgcgggg cgagtgcccc gggcggccgg gtcgcccgct
     7621 gccagccggg accggccgcc agcaggacgg ggcggacccg ggcgccgcgg gtgccccagg
     7681 cggtggcggc gacggcccgg gccggggccg ggtcggcggt ggcccgggtc tgcgaccaga
     7741 gcaggacggc ggccgggccg gtgcgccgga ccgcctccgc cagcgccccg gcgggcagcg
     7801 cggcgccgaa gatccggaac ggcaggccgc gttcggcgag cccggcggcg accgcctcca
     7861 gcgccagggt gtgctgctcg ccgggcatcg cggccagcag caccggcgac ccgcccgcgt
     7921 cgaccggcgg ttcggcgacc cggcgcagcg cactggagac gtgccaggac agcaggtgct
     7981 cgacctcgac gtaccgctcg ccgccgtccg ccgcccaccg gcgcccggcg gcccgcagcg
     8041 cgggcgccat cacgtccgtc caggcggcga ccgcgccgag ccgctcgacg ccggtgcgca
     8101 gcagcccggc cacctcggcg ctgtccagcc gccgcgcggc ccgggccagg ccgcgcacct
     8161 cgggacggac ctccccggcc ggggccggca cggcgggcga cgggaccagc ggctgcggga
     8221 ccagcggctg cgcggcgggc ggcgaggcgg gagcagccgg cggggcgggg tccgtcggcg
     8281 ccgggaggag cacggccggg cccagggcga gccgggcggc ctcggcgggg ggcaggccgc
     8341 tcgcggtgag ccggcacatc tcctccagca ccgcgacgtc ccgcgcggtc cagcgccggt
     8401 gccgcccggc cgcccgctcg gccgggccga tcccgtaacg gcgttcccag gagcggacgg
     8461 tggtcggcga caccccgagc cgccgggcga cctcgccggt gctcgccccg gcctcgggcg
     8521 gttctactgg catcccgtca gcctaggcgc tgggggccgc cgcccggcgg gcttcggccg
     8581 gttcgcgcgg ttcggggccg gggcgcgggg agcggtccca tccgttcccg tggcggctcc
     8641 gaacagcggt gcggatccgc ccttgaaaag tctctcaatg attgagatat cctccggcca
     8701 tgccgacgcc cgcaccgccc gcatccccgc ccgccgcccc gtccgcgccg cccggcgcct
     8761 ccggacggct ccgcttcgtg ctgccgctcg tcctgctgct ggtctggctg gcgctcggcg
     8821 gcgcgctcgg gccgttcgcc ggacggctcg gcgaggtgtc gaccaacgac ggcgcggcgt
     8881 tcctgccgcg cagcgccgag tcgacccggg tcgcgcagct ggagcaggac ttccgcaccg
     8941 agtcgacgct gccggtggtg ctggtctggg agtcctccgc cgggcaggtc gacgacgcgc
     9001 tgcggaccgc cgccggccgg gcgctggcgg ccgccgccga ggtgcccggc gccaccgggc
     9061 cgacctcgcc gctgctgccg tccgaggacg gccgggcgct ggaggcggtg gtgcaactgc
     9121 gcgccgacct cggaccggag accaagaccg cggtcaaggc gatccgcgac ctggccgggc
     9181 aggtcccggg cgcggtcgcc ggagtggccg gaccggccgc ggtgctggcc gacctgtcgg
     9241 gcgcgttcgc cggcatcgac ggcctgctgc tgggcgtcgc gctcggcgtg gtgctgctga
     9301 tcctgctgct ggtctaccgg gcggtactgc tgccgctgct ggtgatcctg ggcgcggtgt
     9361 tcgccctcgc cacggcctgc gcgatcgtgt acgcgctggc ccggcacggc gtcgtccggg
     9421 tcgacgggca ggtgcagggg ctgctgtcga tcctggtgat cggcgccgcc accgactacg
     9481 ggctcctgta ctccgcccgc ttccgcgagg aactggccgc cggacgggac aggtacgcgg
     9541 ccgcccgcac cgcgctgcgc cgctcggtcg aaccggtcgc cgcctccggc ctgaccgtcg
     9601 gcctgggcct gctcgtcctg ctgctctccg acctgaccaa caaccgggcg ctcggcccgg
     9661 tcggcgcgat cggcatcgcc tgcgccgtgc tctccgcgct gaccttcctg cccgcggtgc
     9721 tcgcgctgtg cggccgggcc gcgttctggc ccgcccggcc caaggccgac ggcgccggcg
     9781 gcgcgcaccg gatctggcag cgggtcgccg acctggtcgg ccgccgcccg cgcgcgctgt
     9841 ggatcggctc ggccgccgtc ctggcggcgc tcgccgcctg ctccccgctg ctggacgccc
     9901 ggggcgtgcc gcagtccgaa ctgttcacca ccgcccagcc ctcggtcgcc gcgcagcgcc
     9961 tgctctccgc gcacttcccc ggcggctccg gcaacccggc cgtgatcgtc gccgacgccg
    10021 cgcacgcgga cgccgtcacc gccgccgccg cgcaggtgcc cggcgtcgcc tcggtccgcc
    10081 cgtacaccgg cggcgccccg accgccccgc cggtcgtcac cgccgccggc ctggtccggc
    10141 tcgacgccac gctcgccgac cccgccgaca gcgacgccgc cctcgccacc gtcgcccggc
    10201 tgcggaccgc cgtgcacgcc gtggacggcg ccgacgccct ggtcggcggc tactccgcgc
    10261 agcaggccga cacccggacc accgcccggc acgaccggac gctgatcatg ccggtcgtgc
    10321 tgctctgcat cctcgccgtg ctcgtcctgc tgctccgctc ggtgctggcg ccggtcctgc
    10381 tggccgcgac cgtcgcgctc tcctacctgg ccacgctggg cgtctccgcg ctggtcttcc
    10441 cgcacgtgtt cggcttctcg gcgaccgacc cgtccgtccc gctgtacggg ttcgtcttcc
    10501 tggtcgcgct gggcgtcgac tacaacatct tcctgatgac ccgcgtccgc gaggagaccg
    10561 cggccctcgg cacccgggcc ggcgtcctgc gcggcctcac cgcgaccggc ggcgtcatca
    10621 cctccgcggg cgtcgtcctc gccgcgacct tcgccgccct gggcgtcatc ccgctcgcct
    10681 tcctggccca gatcgccttc atcgtggcct tcggcgtcct gctcgacacc ctcctcgtcc
    10741 gctccctgct cgtccccgcc ctggtccacg acctcggcgg ccgcacctgg tggccggccc
    10801 g
//
