LOCUS       NC_016109              10528 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Kitasatospora setae KM-6054, complete sequence.
ACCESSION   NC_016109
VERSION     NC_016109.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora setae KM-6054
  ORGANISM  Kitasatospora setae KM-6054
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1
  AUTHORS   Ichikawa,N., Oguchi,A., Ikeda,H., Ishikawa,J., Kitani,S.,
            Watanabe,Y., Nakamura,S., Katano,Y., Kishi,E., Sasagawa,M.,
            Ankai,A., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Nihira,T., Horinouchi,S., Ohnishi,Y., Hayakawa,M.,
            Kuzuyama,T., Arisawa,A., Nomoto,F., Miura,H., Takahashi,Y. and
            Fujita,N.
  TITLE     Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary
            snapshot of the family Streptomycetaceae
  JOURNAL   DNA Res 17 (6), 393-406 (2010)
   PUBMED   21059706
  REMARK    DOI:10.1093/dnares/dsq026
REFERENCE   2  (bases 1 to 8783278)
  AUTHORS   Ichikawa,N., Watanabe,Y., Nakamura,S., Katano,Y., Oguchi,A.,
            Ankai,A., Yashiro,I., Fukui,S., Hashimoto,Y., Kamata,S., Otoguro,M.,
            Tanikawa,S., Ikeda,H., Ishikawa,J., Horinouchi,S., Ohnishi,Y.,
            Hayakawa,M., Nihira,T., Kitani,S., Kuzuyama,T., Arisawa,A.,
            Nomoto,F., Miura,H., Takahashi,Y. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2009) Contact:Director-General Department of
            Biotechnology National Institute of Technology and Evaluation, NITE
            Genome Analysis Center (NGAC), Department of Biotechnology; 2-49-10
            Nishihara, Shibuya-ku, Tokyo 151-0066, Japan URL
            :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000269985.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:32:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,713
            CDSs (total)                      :: 7,605
            Genes (coding)                    :: 7,487
            CDSs (with protein)               :: 7,487
            Genes (RNA)                       :: 108
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 25 of 118
            Pseudo Genes (incomplete)         :: 95 of 118
            Pseudo Genes (internal stop)      :: 9 of 118
            Pseudo Genes (multiple problems)  :: 11 of 118
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:33:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 939549
            Orig. end                         :: 950077
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010968.1.
            'Otoguro, M. is at NITE Biological Resource Center (NBRC). Ikeda,
            H., Miura, H. and Takahashi Y. are at Kitasato Institute for Life
            Science. Ishikawa, J. is at National Institute of Infectious
            Diseases. Horinouchi, S., Ohnishi, Y. and Kuzuyama, T. are at The
            University of Tokyo. Hayakawa, M. is at University of Yamanashi.
            Nihira, T. and Kitani, S. are at Osaka University. Arisawa A. is at
            Mercian Corp. Nomoto F. is at Nagase & Co. Ltd. The other authors
            are at NITE Genome Analysis Center (NGAC).
            Please visit our web site. URL:http://www.bio.nite.go.jp/
            DOGAN ; Database of Genomes Analyzed at NITE. The database contains
            genome sequence data, physical maps and the genomic and proteomic
            data of the microorganisms analyzed.
            URL:http://www.bio.nite.go.jp/dogan/Top
            The microbial strain and microbial genomic DNA clones used for the
            sequencing project are available through the NBRC.
            URL:http://www.nbrc.nite.go.jp/e/index.html.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10528
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10528
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10528
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5528](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5528
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(324..337)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.32"
                     /tool="antismash"
     misc_feature    602..616
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            1055..2047
                     /locus_tag="KSE_RS04100"
                     /old_locus_tag="KSE_08500"
     CDS             1055..2047
                     /EC_number="1.1.1.28"
                     /GO_function="GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019327965.1"
                     /locus_tag="KSE_RS04100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_08500"
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="WP_014134008.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.5e-51, bitscore:
                     173.0, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEILAYGVLSDERPLLQDALEQHRLRMLDVFLTADTAPLAAGFPV
                     VSSSVNDTLDAGVLSLLAAGGTKLIAQRSTGFNNIDLTAAAELGLTVARVSYYSPYAVA
                     EHAWTLALAVNRRLTRATTRSREFDFRLDGLMGRDIHGMTVGVIGTGKIGECFARIAAG
                     FGTELLGWDVAENPACLELGMTYTELPELLSRADLVSLHVPLLPATHHLIDAAALARMK
                     DDAILVNSSRGGLVDSTALVETLRAGRLSGVGLDVYEEETGVFFTDQSIQGVTDDTLAR
                     LVTFPQVLVTSHQAYYTRTAVGQIIDATARNIDDFAAGRTSENTLVPKS"
     gene            2451..2774
                     /locus_tag="KSE_RS04110"
     CDS             2451..2774
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958287.1"
                     /locus_tag="KSE_RS04110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_051055097.1"
                     /transl_table=11
                     /translation="MNVPELLRLPQAEHDESAARADHLREQIDQLTSAPAEVEARLAGM
                     VTSGKVIDGLGLLDNEPATAETAPVHQRIVTAFNEDPRQVFRVGEVHELLDQPGRSRYW
                     KRT"
     gene            2990..4174
                     /locus_tag="KSE_RS38105"
                     /old_locus_tag="KSE_08540"
     CDS             2990..4174
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSE_RS38105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSE_08540"
                     /product="hypothetical protein"
                     /protein_id="WP_148283056.1"
                     /transl_table=11
                     /translation="MSKMTERIVLPLPELLPRGRAEFREAARLMAAAGLEVDLLSGPGQ
                     DLRRARGKITALYEGSLVELLLHPAGRGWSAPAREQDRFFGVVRATGDAGGILLGGVLL
                     SYVEAVEHSRATGCSLSEQDWRDLFRGPTEMIRFSSGRALSADGPTPEQPRTGTGNGEE
                     RPGIRWEVGHRLFFSLTQSITTALSCFAAATARRDEAESADSLELATSLMTAAAAALRF
                     TADFSPAHYENDVRPSMMPPHVEPGFSGVQGRDHQVLVEVLRALRPVFGSIDRARYPYD
                     SFKSAAERLFAAHEFVCAKFGGETKPSLLMEAHHKDGSSTSAAAAVVRQMSERRMALIH
                     PHRPSREQHSPGARGGADEPSEPISASGPDGGRSRRRVGDSEDRGGVGGGAPPT"
     misc_feature    3023..3025
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4349..4885
                     /locus_tag="KSE_RS04120"
                     /old_locus_tag="KSE_08550"
     CDS             4349..4885
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134011.1"
                     /locus_tag="KSE_RS04120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_08550"
                     /product="hypothetical protein"
                     /protein_id="WP_014134011.1"
                     /transl_table=11
                     /translation="MTGFRRLVPPLALSLVSLGALVTSGGSARAADPPAVVCTTTATQH
                     YSPGLTLVSRPTRITASATYACTGAGSVDTARLGFDYTVDAGCLPTSSPVTTGISVLDW
                     NEGPDSEITSTFVVTRPAGQTVGTSVGTVTAGQFNGYLVHLVGTYPGSDPLACATPQGV
                     TSSQGELVLTLTQPF"
     gene            5001..5528
                     /locus_tag="KSE_RS04125"
                     /old_locus_tag="KSE_08560"
     CDS             5001..5528
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.5"
                     /locus_tag="KSE_RS04125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_08560"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_014134012.1"
                     /sec_met_domain="AfsA (E-value: 6.8e-07, bitscore: 28.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIASCVLRAPDGRVVADGETTAGVLEGTLYRNWRGAGVVRAGQLP
                     TAPGEPRARAGRVRSRPENQVLARIGREDGGAHAVLRLSGPHPYLLPASADHVTGRLFA
                     EATRQLVLGACAPDGNGVRTLHLSFPRLCTLDAPVRVRAVPCPRSPASARWRVLFSQAG
                     ATVCTGRVETGV"
     misc_feature    complement(5002..5017)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.33"
                     /tool="antismash"
     misc_feature    5766..5784
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.71"
                     /tool="antismash"
     gene            6269..7096
                     /locus_tag="KSE_RS04130"
                     /old_locus_tag="KSE_08570"
     CDS             6269..7096
                     /NRPS_PKS="Domain: Abhydrolase_1 (21-254). E-value:
                     6.2e-38. Score: 122.7. Matches aSDomain:
                     nrpspksdomains_KSE_RS04130_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134013.1"
                     /locus_tag="KSE_RS04130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_08570"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_014134013.1"
                     /sec_met_domain="PF00561 (E-value: 4.3e-30, bitscore:
                     104.0, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.3e-21, bitscore:
                     76.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPHCTTRDGVEIFYKDWGSGRPVVFIHGWPLNGDAWQDQLKAVAD
                     AGYRGIAHDRRGHGRSTPVWDGYDFDTFADDLNDLLTSLDLRDVTLVAHSMGGGELARY
                     IGRHGTGRVRSAVLLSAIPPLMLQGPDNPEGVPESVFDGIKAGIVNERSQFWKDTAVGF
                     LSANRPGTKATEGNKDAFWYMAMAETIQGGVACVDAFAYTDFHEDLKKFDIPLLVVHGD
                     DDQVVPIDATGRKTAKLVPGAVLKVYEGGSHGIAVVPGDKERFNQDLLDFLKT"
     misc_feature    6296..6312
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.43"
                     /tool="antismash"
     misc_feature    6302..6315
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.72"
                     /tool="antismash"
     aSDomain        6332..7030
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KSE_RS04130_Abhydrolase_1.1"
                     /evalue="6.20E-38"
                     /label="KSE_RS04130_Abhydrolase_1.1"
                     /locus_tag="KSE_RS04130"
                     /protein_end="254"
                     /protein_start="21"
                     /score="122.7"
                     /tool="antismash"
                     /translation="PVVFIHGWPLNGDAWQDQLKAVADAGYRGIAHDRRGHGRSTPVWD
                     GYDFDTFADDLNDLLTSLDLRDVTLVAHSMGGGELARYIGRHGTGRVRSAVLLSAIPPL
                     MLQGPDNPEGVPESVFDGIKAGIVNERSQFWKDTAVGFLSANRPGTKATEGNKDAFWYM
                     AMAETIQGGVACVDAFAYTDFHEDLKKFDIPLLVVHGDDDQVVPIDATGRKTAKLVPGA
                     VLKVYEGGSHG"
     gene            <7109..8218
                     /locus_tag="KSE_RS04135"
                     /old_locus_tag="KSE_08580"
                     /pseudo=""
     CDS             <7109..8218
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133656.1"
                     /locus_tag="KSE_RS04135"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_08580"
                     /product="peroxidase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="PAMLTTDVAMRVDPDYARICHRFLQHPEEFADAFARAWYKLTHRD
                     MGPITRYLGPEVAAEHLIWQDPLPEPAEAPLDADAVGQLKSRVLAAGIPVADLVWLAWS
                     CASTFRRSDKRGGANGARIRLEPQNGWEVNEPHRLAGLLARLEEIRTAFASSRPAGHGV
                     SLADLIVLAGTAAVEKAAADAGHELRVPFTPGRVDATREHTDIDGFSHLEPTADGFRNH
                     LGKDDTLPAEYRLVDRADLLGLSARELTALVGGLRTLGATYQSSDLGVLTARPGVLTND
                     WYVNLLDQDTTWTPTAHDPALFEGRDARGSLRWTASRTDLVFGSNAELRALAEVYASAG
                     GEQQFATDFAAAWAKVMDLDRYDLPSHHS"
     gene            8273..8773
                     /locus_tag="KSE_RS04140"
                     /old_locus_tag="KSE_08590"
     CDS             8273..8773
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134015.1"
                     /locus_tag="KSE_RS04140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_08590"
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_014134015.1"
                     /transl_table=11
                     /translation="MTDPDAQDPLPAEDDGVLDPADSLETDDLDDDPLETGIVTADGYR
                     GATEYGTTAAEAATGESLDRLLAEEEPDTPAPDVDDRWSGGPGPRAGRLVEAGTDLFAE
                     DGGADGGAAGAEEAAVHLTDEDADEAAEFDDGDDEESLADALRFTAADDLRDADPDAGD
                     DYR"
     gene            8898..9071
                     /locus_tag="KSE_RS42675"
                     /old_locus_tag="KSE_08600"
     CDS             8898..9071
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSE_RS42675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSE_08600"
                     /product="hypothetical protein"
                     /protein_id="WP_014134016.1"
                     /transl_table=11
                     /translation="MDLSLRRPVQQVSDECAEDSQRCYHRAVVGRIIDATARNIDDYAA
                     GRTSGNTLVPKS"
     gene            9279..10235
                     /locus_tag="KSE_RS04145"
                     /old_locus_tag="KSE_08610"
     CDS             9279..10235
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134017.1"
                     /locus_tag="KSE_RS04145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSE_08610"
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="WP_014134017.1"
                     /transl_table=11
                     /translation="MHHHGGGPLPPFSFSRALEWSPDWPYLVFCALALGLYLAGAVRLW
                     RRGDKWPAGRVVAWTAGVGTILLVTNTGLNDYGMVLFSAHMTQHMVLSMLSPILLLLGA
                     PITLALRSLRPARKGSGKGPRELLVALLHSTYVRIVSHPAFTIPAFIASLYVLYFTPLF
                     DFLMQYRLGHLAMMLHFLAVGMLFFWPIMGVDPGPHRPGFVMRIIELFMGMPFHAFFGV
                     AVMMASGQLVSTFSAETAPLGTNLHDDQQLAGGITWAFGEIPTAIVLIALTLQWAKSEE
                     RQARRRDRAADRDGDAELVAYNQYLASLDKAANRTAG"
ORIGIN
        1 tcctgggctc ctcgcgggtc cagcccagcc acctgcgcga cggcgtggag cgcgccaagc
       61 agcactgcgc ggacctcggc atcgacgcgg tcatcccgat cggcggcgag ggcaccctga
      121 aggccgcgaa gctgatgagc gacgcgggcc tgccggtggt cggcgtgccg aagaccatcg
      181 acaacgacat cgcctgcacc gacgtcacct tcggcttcga caccgccgtc tcggtggcca
      241 ccgaggcgct ggaccggctg aagaccaccg ccgagtcgca ccagcgggtg atggtcgtcg
      301 aggtgatggg ccggcacacc ggctggatcg cgctcaacgc gggcatggcg gccggtgcgc
      361 acgccatcat cgtgccggag cggccgttcc acatcgacaa gctgaccgag gtcgtgcgcg
      421 agcgcttcga ccggcagaag aagttcgcga tcgtggtctg cgccgagggc gcgaagcccg
      481 agcccggcac catgccgtgg gaggagggca ccaaggacat gtacggccac gagcgtttca
      541 ccggcatcgc cacccagctc tcccgcgagc tggagcaccg cctcggcaag gaggcccgcc
      601 cggtcatcct cggccacacc cagcgcggcg gcaccccgac cgcgtacgac cgcgtcctgg
      661 ccacccgctt cggctggcac gcggtggagg ccgcccacaa gggcgccttc gggcacatca
      721 cggcgctgca gggcaccgag atcaagctgg tcccgctcgg cgaggccgtg gccgagctga
      781 agaccgttcc ggcggagcgc tacaaggagg ccgagaccgt catctgacgg gcgcggtcgg
      841 ccgagcggtc gcagtggccg cagtggccgc actgcttgca gcggtcgcag tgaatgatcc
      901 gatcatcaca agatgatgga acgatgtatc gcccgtcgat catcggaccg tcatcctgtg
      961 atgatcggat cttcgctttc ccggacctct tcgcctcccc cgcccggggc gcgtcgcgcc
     1021 cggcggcgca cgcccccggc gatagcctcg cggcatggag atcctcgcct acggcgtcct
     1081 gtccgacgaa cgcccgctgc tccaggacgc gctggaacag caccggctgc gcatgctgga
     1141 cgtcttcctc accgccgaca ccgccccgct ggccgccggc ttcccggtcg tctccagcag
     1201 cgtcaacgac accctggacg ccggggtgct gtcgctgctc gcggccggcg gcaccaagct
     1261 gatcgcccag cggtccaccg gtttcaacaa catcgacctg accgcggccg ccgaactcgg
     1321 cctgaccgtc gcccgggtct cctactacag cccgtacgcg gtcgccgagc acgcctggac
     1381 cctcgccctc gccgtcaacc gccggctcac ccgggccacc acccgctccc gcgagttcga
     1441 cttccggctc gacggcctga tgggccgcga catccacggc atgaccgtcg gcgtgatcgg
     1501 caccggtaag atcggcgagt gcttcgcccg gatcgcggcg ggcttcggca ccgaactcct
     1561 cggctgggac gtcgcggaga accccgcctg cctggaactc ggcatgacct acaccgaact
     1621 ccccgaactc ctctcccgcg ccgacctggt gagcctgcac gtccccctgc tgccggccac
     1681 ccaccacctg atcgacgccg ccgcgctggc ccggatgaag gacgacgcga tcctggtcaa
     1741 ctccagccgc ggcggcctgg tcgacagcac cgccctggtc gagaccctgc gcgccggccg
     1801 cctctccggc gtcggcctcg acgtctacga ggaggagacc ggcgtcttct tcaccgacca
     1861 gtcgatccag ggcgtcaccg acgacaccct cgcccgcctc gtcaccttcc cccaggtcct
     1921 ggtcaccagc caccaggcct actacacccg caccgccgtc ggccagatca tcgacgccac
     1981 cgcccgcaac atcgacgact tcgccgccgg ccgcaccagc gagaacaccc tggtgccgaa
     2041 gagctgaccc agcacccgtc cggccaacgg ccccacctcg ctccccgggg gtgtccagcg
     2101 gccggacccc ccgcccgtcg aaccggttca agcccaggga atggatgccg tactcgacgg
     2161 actcgtccga cgaacagcgg gcccttggtc gagccgttgg tcaccgcctg gaagcagaag
     2221 cgggtgttgt gctcggcgac cggggacccg ggctcgtgcg atctgcgcga ggtcgtgaac
     2281 gcgctgctgt accggagccg gacgggctgc cagcggcggc tcccaccgca tgactcaccg
     2341 gtactcaccg gtctggtcgg cggtgttcca ctcccatgct ccaccgccgg catgctccgc
     2401 cacctcacca cccccacccc cgcctggcgc gacgaggccg agccggccgc gtgaacgtcc
     2461 ccgaactgct gcggctgccg caggccgaac acgacgagag cgccgcacgc gccgatcacc
     2521 tgcgcgaaca gatcgaccaa ctcaccagtg cgccggccga ggtcgaggcc cgcctcgcgg
     2581 ggatggtcac cagcggcaag gtcatcgacg gcctcggcct gctcgacaac gaaccggcca
     2641 ctgcggagac cgcccccgtc caccagcgca tcgtgaccgc cttcaacgag gatccccggc
     2701 aggtcttccg agtcggtgaa gtacacgaac tcctcgacca gcccggacga agccgctact
     2761 ggaaacggac ttaacgccct ctgaaactta gcgagaatcc caccctggct tctggtcgtc
     2821 ggcccctcac ggggcaacca tgagaatgtc cttccggcgc ccgaggagcc atcccccgct
     2881 cccgtgcaca cggacagtcg gcaacgtgcc cagcagcgtc ttcaccgtca ccggacggac
     2941 cccgggcacg aaccggacag caacagcaag atgaagagtc ctcatgatca tgagcaagat
     3001 gaccgagcgg atcgtcctcc cattacccga actcctaccg cggggccggg ccgagttccg
     3061 agaggcagca cggctgatgg ccgcggccgg actggaggtc gacctgctga gcgggcccgg
     3121 ccaggaccta cgccgcgccc ggggaaagat cacggctctg tacgagggtt cactggtcga
     3181 gctcctgctc catccggccg gtcgaggatg gagtgcgccg gcccgtgaac aggaccgctt
     3241 cttcggcgtg gtgcgggcaa cgggagatgc tggcggcatc ctgctcggcg gcgtcctgct
     3301 ctcctacgtc gaggccgtcg agcactcccg ggcgaccggc tgctcgctgt cggaacagga
     3361 ctggcgggac ctgttccgag gcccgaccga aatgatccgc ttctcttccg ggcgggctct
     3421 ctccgctgac ggcccgacgc ccgagcagcc gcgaacggga acggggaacg gggaagaacg
     3481 ccccggaatc cgctgggagg tgggtcaccg tctcttcttc tccctcaccc agagcatcac
     3541 cacggccctg tcctgcttcg ccgcggcgac ggcacggagg gacgaggccg aatccgcgga
     3601 ctcactcgaa ctggccacct ccttgatgac cgcagccgct gccgcgttgc ggttcaccgc
     3661 cgacttctcc ccggcgcact acgagaacga cgtcagaccc agcatgatgc cgccgcacgt
     3721 ggaacccggg ttcagcggcg tacaaggtcg cgatcaccag gtgcttgtcg aggtgctccg
     3781 cgcgctgcgc ccggtcttcg gctcgatcga ccgcgcacgc tatccctacg acagcttcaa
     3841 atccgccgcc gagcggctct tcgcggcgca cgagttcgtc tgcgccaagt tcggcggtga
     3901 gaccaagccc agcctcctca tggaggcgca ccacaaggac gggagcagca ccagcgcggc
     3961 ggcggctgtc gtgcgccaga tgtcggaacg ccggatggcc ctgatccacc ctcaccggcc
     4021 gtcgcgggag cagcactcac ccggtgcccg gggtggcgcg gacgaaccct cggagccgat
     4081 cagcgcgagc ggtccggacg gcgggcgcag caggaggcgg gtcggcgatt cggaggaccg
     4141 cggaggcgtt ggcggcgggg caccaccgac gtgaactgac cacgcgtcac agctccccag
     4201 ggggctccgg atgccgatgg tgttccaccg gaggtggacc gggtctctgg ggcagcgctg
     4261 gtcggagccc cgggttcgac ggggcggtcg aggaggggag ggtgcgcccc cgcgcccggt
     4321 gtgctcccca ctccctcctg gagaagccgt gaccggtttc cgccgcctcg tcccgccgct
     4381 cgcgctgagc ctcgtgtcgc tcggggcgct cgtgacctcc ggcggttcgg cccgggccgc
     4441 cgacccgccg gccgtcgtct gcacgaccac cgccacgcag cactacagtc ccgggctcac
     4501 gctcgtctcc cgcccgacac gcatcacggc gagcgccacc tacgcctgca cgggcgccgg
     4561 ttcggtggac acggcccggc tcggcttcga ctacaccgtg gacgccggct gccttccgac
     4621 ctcgtccccg gtgacgaccg ggatcagcgt cctggactgg aacgaggggc ccgactccga
     4681 gatcacctcc accttcgtgg tcaccaggcc cgcgggccag accgtgggga cctccgtcgg
     4741 cacggtgacg gcgggccagt tcaacggcta cctcgtccat ctcgtcggca cgtaccccgg
     4801 atcggacccg ctcgcctgtg cgacccccca gggggtcacg tccagccagg gagagctggt
     4861 cctcacgctc acccagccct tctgagccgt cgcccgtccc gcgcgggacg ggccggcccc
     4921 tgcgccccct ccccggggga ctgctggcga cctgccttgt cgaacagctc cggacgagcc
     4981 ggacgggacg cacccggcgc gtgatcgcct cctgcgtcct ccgggcgccg gacggacggg
     5041 tggtggcgga cggcgagacg accgcggggg tcctggaggg caccctctac cggaactggc
     5101 ggggagccgg agtcgtccgg gccggacagc tgccgaccgc cccgggcgaa ccccgtgccc
     5161 gcgcggggcg ggtcaggtcc cggccggaga accaggtcct ggctcggatc gggcgggagg
     5221 acgggggcgc ccatgccgtg ctgcgcctgt ccggtccgca cccctacctg ctgcccgcgt
     5281 ccgccgacca cgtgaccggg cggctgttcg cggaggcgac ccggcagctc gtgctcggtg
     5341 cgtgcgcgcc ggacggcaac ggagtgcgaa cgctgcacct gtcgttcccc cggctgtgca
     5401 ccctggacgc ccccgtgcgg gtgcgcgccg ttccgtgccc gcggtcaccg gcgtccgccc
     5461 gatggcgcgt cctcttctcc caggcgggcg ccaccgtgtg caccgggcgc gtcgagacgg
     5521 gggtctgacc gacgcgcagg tgcggcgcga gcgttcccgt gcggccggaa cccgaagcgc
     5581 gcggcccgcg tcaccgtgtc ggtcggctgc ccgccccgga cgtggccctg cggggcccga
     5641 acacggcgtg gtgtgccgac cggagcgccg gcacatcacg gcggacccgc cgccggcccc
     5701 gcggccgtgg ccagccgagg accgtacgga gcttcgcgct caccggcatc gcggcgctca
     5761 cgctgctgga gaacgccccg caagcccgct cgccggccgg tacctcgttc ccgggctgat
     5821 cggggccggg gctgatcggg gccggggctg atcggggccg gcgtcctggt cctcacccgc
     5881 gtcgttccgc acatccgggg cccggccgac tgagcccggc cgggcctcca ggccctcgcg
     5941 ggtgggtctc cggggacccg gtgcgagggt tctggcgagt gggcgcaccg tacccgtggc
     6001 cgcctcccct cggaggcggt gcggtagtgg cgggggcgcg gggcgggaaa gccgccgagc
     6061 cgccccgtga acacgcaggg tcgtcgcccg gtcccccggg ggacaccctg tcggcgcagt
     6121 ggcgcggtgt gtcggatgca gggcgtagcg gtcgtggggt tggctgggtc ccgcgtcgac
     6181 ccccgttccg cctccgtact gcacgggggc cggtgcggct tccggtcggc acgtccggcc
     6241 gttcccgaga ccgaatccgg agtcactcat gccccactgc accacccgcg acggcgtgga
     6301 gattttctac aaggactggg ggagcggccg cccggtcgtc ttcatccacg gctggccgct
     6361 gaacggcgac gcctggcagg accagctgaa ggccgtcgcc gacgccggct accgcggcat
     6421 cgcccacgac cggcgcggcc acggccgctc caccccggtc tgggacggct acgacttcga
     6481 caccttcgcc gacgacctga acgacctgct gaccagcctc gacctgcgcg acgtcacgct
     6541 cgtcgcgcac tcgatgggcg gcggcgagct ggcccgctac atcggccggc acggcaccgg
     6601 ccgggtccgc tccgccgtgc tgctctccgc catcccgccg ctgatgctcc agggcccgga
     6661 caaccccgag ggcgtcccgg agtccgtctt cgacggcatc aaggccggga tcgtcaacga
     6721 gcgctcgcag ttctggaagg acaccgcggt cgggttcctg tccgcgaacc ggcccggcac
     6781 caaggccacc gagggcaaca aggacgcctt ctggtacatg gcgatggccg agaccatcca
     6841 gggcggcgtc gcctgcgtcg acgccttcgc gtacaccgac ttccacgagg acctgaagaa
     6901 gttcgacatc ccgctgctgg tcgtccacgg cgacgacgac caggtcgtcc cgatcgacgc
     6961 caccggccgc aagaccgcca agctcgtccc cggcgccgtc ctcaaggtct acgagggcgg
     7021 ctcgcacggc atcgccgtcg tccccggcga caaggagcgc ttcaaccagg acctgctcga
     7081 cttcctcaag acctgacccc gccggacccc ggcgatgctg accaccgacg tcgcgatgcg
     7141 cgtcgacccc gactacgccc gcatctgcca ccgcttcctc caacaccccg aggagttcgc
     7201 cgacgccttc gcccgcgcct ggtacaaact cacccaccgc gacatgggcc cgatcacccg
     7261 ctacctcggc cccgaggtcg ccgccgagca cctgatctgg caggaccccc tccccgagcc
     7321 cgccgaggcc ccgctcgacg ccgacgccgt cggacagctc aagtcccgcg tgctggccgc
     7381 cgggatcccg gtcgccgacc tggtgtggct cgcctggtcc tgcgcgtcga ccttccgccg
     7441 cagcgacaag cgcggcggcg ccaacggcgc ccggatccgg ctggagccgc agaacggctg
     7501 ggaggtcaac gagccccacc ggctggccgg cctgctcgcc cggctggagg agatccgcac
     7561 cgcgttcgcc tcctcccggc ccgccggcca cggcgtctca ctcgccgacc tgatcgtgct
     7621 ggcgggcacc gccgccgtgg agaaggccgc cgccgacgcc gggcacgagc tgcgggtgcc
     7681 gttcaccccc ggccgggtcg acgcgacccg ggagcacacc gacatcgacg gcttctccca
     7741 cctggagccg accgccgacg gcttccgcaa ccacctcggc aaggacgaca ccctgcccgc
     7801 cgagtaccgc ctggtcgacc gcgccgacct gctcgggctg agcgcccggg aactgaccgc
     7861 gctggtcggc ggcctgcgca cgctcggcgc gacctaccag agcagcgacc tgggcgtgct
     7921 caccgcccgc cccggcgtcc tgaccaacga ctggtacgtc aacctcctcg accaggacac
     7981 cacctggaca cccaccgccc acgaccccgc cctcttcgag ggccgcgacg cccggggctc
     8041 cctgcgctgg accgccagcc gcaccgacct cgtcttcggg tcgaacgccg aactgcgggc
     8101 cctcgccgag gtctacgcct ccgccggcgg cgagcagcag ttcgccaccg acttcgccgc
     8161 ggcctgggcc aaggtcatgg acctcgaccg ctacgacctg cccagccacc acagctgacc
     8221 gcggttgccc ctctccgggg cgcccacccg ttccgttccg aaagggagtg ccgtgaccga
     8281 tcccgacgcc caggacccgc tgcccgccga ggacgacggc gtcctcgacc cggcggactc
     8341 gctggagacc gacgatttgg acgacgaccc gttggagacc ggcatcgtca ccgccgacgg
     8401 ctaccgcggc gccaccgagt acggcaccac cgccgccgag gccgccaccg gggagtccct
     8461 ggaccggctg ctcgccgagg aggagcccga cacgcctgcc ccagacgtcg acgaccgctg
     8521 gtccggcggc ccgggccccc gggccggacg gctggtggag gcggggacgg acctgttcgc
     8581 cgaggacggc ggcgcggacg gcggcgcggc gggcgccgag gaggccgccg tccacctcac
     8641 cgacgaggac gccgacgagg ccgccgagtt cgacgacggc gacgacgagg agtccctggc
     8701 cgacgcgctg cggttcacgg cggcggacga cctgcgcgac gccgacccgg acgccgggga
     8761 cgactaccgc tgactcccgg tcctcgggtc cgccgggctg cggaccgacg tgcggcaccg
     8821 cgggacccgg cctttcacgg gagctgcggc gcaccgccac ggcggccacg tcgcaccccc
     8881 acccgatcgg gatcgcgatg gacctgtcgc tgcgtcgtcc cgtccagcag gtcagtgacg
     8941 agtgcgccga ggactcccag cgctgctacc accgggccgt ggtcggccgg atcatcgacg
     9001 ccaccgcccg caacatcgac gactacgccg ccggccgcac cagcgggaac accctggtgc
     9061 cgaagagctg accccgggcc gggcccgggc ggcgggggcg cgggcccggg cggcgggggc
     9121 gcccgcccgg gcgcggtaag cgcggcttag gctggggccc gaacgaaacc ggaccaaacc
     9181 ggaccgggcc acaccgggcc gccccgggcc gccgctcggg cggcgccggg cggcatggac
     9241 gaaagcgggg ccgcccgatg ggcgacggca tgacggggat gcaccaccac ggaggcggac
     9301 cactcccccc cttctccttc tcccgcgccc tggagtggtc tccggactgg ccctacctgg
     9361 tgttctgcgc gctcgccctc ggcctgtacc tggcgggggc ggtccggctg tggcggcgcg
     9421 gcgacaagtg gccggccggc cgggtggtcg cctggaccgc cggtgtcggc accatcctgc
     9481 tggtcaccaa caccgggctg aacgactacg gcatggtgct gttcagcgcg cacatgacgc
     9541 agcacatggt gctctccatg ctgtcgccga tcctgctgct gctgggcgcc ccgatcaccc
     9601 tcgcgctgcg ctccctgcgc ccggcccgga agggcagcgg gaaggggccg cgcgaactgc
     9661 tggtggcgct gctgcactcc acctacgtgc ggatcgtctc gcacccggcg ttcaccatcc
     9721 cggcgttcat cgccagcctg tacgtgctgt acttcacgcc gctgttcgac ttcctgatgc
     9781 agtaccggct cgggcacctg gcgatgatgc tgcacttcct cgccgtcggc atgctgttct
     9841 tctggccgat catgggcgtc gaccccggcc cgcaccgccc cggcttcgtg atgcggatca
     9901 tcgagctgtt catgggcatg ccgttccacg cgttcttcgg cgtcgcggtg atgatggcct
     9961 ccgggcagct ggtctccacc ttcagcgccg agacggcgcc gctcggcacc aacctgcacg
    10021 acgaccagca gttggccggc ggcatcacct gggcgttcgg cgagatcccg accgcgatcg
    10081 tgctgatcgc gctgaccctg cagtgggcca agtccgagga gcggcaggcc cgccgccgcg
    10141 accgggccgc ggaccgggac ggcgacgccg agctggtggc gtacaaccag tacctggcct
    10201 cgctcgacaa ggccgccaac cgcacggcgg gctgagcccc cgtcctcgtt cgtcccccgt
    10261 cctcctacga cagcgaaccg cccggaacgg cctcccgttc cgggcggttc gtcgtgcgcg
    10321 gggcctgccg gtcagccgat gccctggcgg tcgggggcga gggcgaaggc gcggttgtcg
    10381 tcgtcgcgga cggactcggc gacggcgcgg gtgagcaggt ggcgctggag cagcagcggg
    10441 gggcggcgcg gttcgggggt gatccggagc aggtcgtcga gggcggcgac cagccggcgg
    10501 gtgacctggg gctggccggc gccgcaga
//
