LOCUS       NC_018681              10879 bp    DNA     circular CON 21-MAR-2024
DEFINITION  Nocardia brasiliensis ATCC 700358, complete sequence.
ACCESSION   NC_018681
VERSION     NC_018681.1
KEYWORDS    RefSeq.
SOURCE      Nocardia brasiliensis ATCC 700358
  ORGANISM  Nocardia brasiliensis ATCC 700358
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Complete genome sequence of Nocardia brasiliensis HUJEG-1
  JOURNAL   J Bacteriol 194 (10), 2761-2762 (2012)
   PUBMED   22535940
REFERENCE   2  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
REFERENCE   3  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000250675.2-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 01:53:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,551
            CDSs (total)                      :: 8,489
            Genes (coding)                    :: 8,417
            CDSs (with protein)               :: 8,417
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 13 of 72
            Pseudo Genes (incomplete)         :: 63 of 72
            Pseudo Genes (internal stop)      :: 3 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:09:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9184796
            Orig. end                         :: 9195675
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003876.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10879
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="51"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10879
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10879
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5879](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5879)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            805..1503
                     /locus_tag="O3I_RS41145"
                     /old_locus_tag="O3I_041040"
     CDS             805..1503
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080112.1"
                     /locus_tag="O3I_RS41145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041040"
                     /product="hypothetical protein"
                     /protein_id="WP_014989000.1"
                     /transl_table=11
                     /translation="MTASRSDSRSSGVRPPGALPALSDIPAEVVPGVLRIENSDKDATT
                     PIIMDMLRSVYPHDQIFGEYCPVQAYIAAPPREVYEYLADTRSLEEWTYSLRGFAETDE
                     PGLWLAYDRLGDETQIFTRTVAHPAAMTVDYHCAWDQSRHLWMIYLLRVIDAQTVFDKP
                     GSVVLWVNCKHPFYDENPYPETAPPRRPVWVGDFWEMFSAGHQLEMDNLKAICEYRAAN
                     GLPVKPDWMN"
     gene            1511..2533
                     /locus_tag="O3I_RS41150"
                     /old_locus_tag="O3I_041045"
     CDS             1511..2533
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727696.1"
                     /locus_tag="O3I_RS41150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041045"
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="WP_014989001.1"
                     /sec_met_domain="fabH (E-value: 1.1e-24, bitscore: 86.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSFPTVSLVDVAGYLPGEPVGTEYFTQFSRSDRMAKNVMFRSPT
                     ARHHVDRDETAVDMVERAVAPLIERHGAQFLSQIDVLITHTQLPTNPVMGCGPEVARRL
                     GARPGFVYDVHNGGCAAFVHMMSMAQMVLQTTDARTALIAATQNCAGPVFTQPEIRKLA
                     QAPVPGDGCGVGLLVKDDSAPILDIECRTYPEFAGDMEFSTNGDRKYWEPGEGQGCVSF
                     TESKITKVFARGNRLVPEVALAVCDRIGVKGRDIDTFVTNQPNRLFLRNWHDALELPAE
                     RHPDTFDRCGNLFAAGIPVTLDVENRAGRLRNGSVVLLSAFAHAGDFAAAAAIRWGAAG
                     "
     gene            2530..3564
                     /locus_tag="O3I_RS41155"
                     /old_locus_tag="O3I_041050"
     CDS             2530..3564
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (24-305). E-value:
                     1.3e-45. Score: 148.0. Matches aSDomain:
                     nrpspksdomains_O3I_RS41155_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212054.1"
                     /locus_tag="O3I_RS41155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041050"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_041564974.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2e-43, bitscore:
                     147.9, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVRFVEETTPSRQRRGAATPPRFDLALSENPFPPLPSVLRAVSV
                     PLAQANRYPEFLPERLPRLIARHLGVRADQLVVGAGATGVAWQIIQSLTTPGAGMVYGS
                     PTFDGYPIMAEMAGLDPVPVPLDAGGDQDLRALAKAVDRRTGLVVVCRPHNPTGTVVPA
                     AELAAFLRAVPARVPVILDEAYVEFLDAADHVDPRVLLSRHANLLVLRTFSKAYGLAGL
                     RIGYAFGRRDLAARVRRLQLPFGMSSTAEAAVAASYAAAPELAARTLRITAAREELRTA
                     LRDRGIAVPRSQANFLFLPGPGVATALRRAGIGAKAYPDGSARISVGDPDADRAVLHAL
                     AGLR"
     aSDomain        2602..3444
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O3I_RS41155_Aminotran_1_2.1"
                     /evalue="1.30E-45"
                     /label="O3I_RS41155_Aminotran_1_2.1"
                     /locus_tag="O3I_RS41155"
                     /protein_end="305"
                     /protein_start="24"
                     /score="148.0"
                     /tool="antismash"
                     /translation="DLALSENPFPPLPSVLRAVSVPLAQANRYPEFLPERLPRLIARHL
                     GVRADQLVVGAGATGVAWQIIQSLTTPGAGMVYGSPTFDGYPIMAEMAGLDPVPVPLDA
                     GGDQDLRALAKAVDRRTGLVVVCRPHNPTGTVVPAAELAAFLRAVPARVPVILDEAYVE
                     FLDAADHVDPRVLLSRHANLLVLRTFSKAYGLAGLRIGYAFGRRDLAARVRRLQLPFGM
                     SSTAEAAVAASYAAAPELAARTLRITAAREELRTALRDRGIAVPRSQANFLFLPGPGVA
                     "
     gene            complement(3561..4565)
                     /locus_tag="O3I_RS41160"
                     /old_locus_tag="O3I_041055"
     CDS             complement(3561..4565)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926978.1"
                     /locus_tag="O3I_RS41160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041055"
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="WP_014989003.1"
                     /transl_table=11
                     /translation="MRFLSKFYIDGFILSILASVALASVFPARGTAADVVDVLTKIAIA
                     LLFLLYGTRLSPKEALAGLTHWRLHLVVLACTFVAFPLLGLAARVLVPSVLTDDLYTGL
                     LFLCLVPSTVQSSIAFTSIAKGNVPGAIVSATTSNLLGVFITPLLVILLMNTTGHASVD
                     FSSIGDIVLQLLVPFAIGQLIRPKVIGWLSRYAEPTKLVDRGSILLVVYAAFSAGMVEG
                     IWHMLSPWRILALAGVCCVLLALIIALTMLIGRFAGFSRADRIVIVFCGSKKSLATGLP
                     MATVLFASQPVGLIVLPLMMFHQIQLITCAALSQRWAKQADDPGETSELKPAA"
     misc_feature    complement(4519..4533)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    complement(4659..4672)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.84"
                     /tool="antismash"
     gene            complement(4690..5004)
                     /locus_tag="O3I_RS41165"
                     /old_locus_tag="O3I_041060"
     CDS             complement(4690..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014361043.1"
                     /locus_tag="O3I_RS41165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041060"
                     /product="hypothetical protein"
                     /protein_id="WP_014989004.1"
                     /transl_table=11
                     /translation="MSAELAAQQAALVRALVAGAPVPAGFDCADVAAAAHALLRKRADE
                     VARRFPMLAQACGPDFRERFLAWADGRSKTSTAADATAFAASQGLPAPVGTTTKRWPRR
                     "
     gene            complement(5001..5879)
                     /locus_tag="O3I_RS41170"
                     /old_locus_tag="O3I_041065"
     CDS             complement(5001..5879)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353594.1"
                     /locus_tag="O3I_RS41170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041065"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_014989005.1"
                     /sec_met_domain="DUF692 (E-value: 1.8e-67, bitscore: 226.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGLLPAAALGIGWRPEICGVIAELDGLGFAEVIAESLPLRRRSR
                     LRGGLTAAPPAELTALIDRGVAVVPHGISLSLGGAEPVGEQRIAHLATAAAALAAPLVS
                     EHIAFVRAGGVEAGHLLPVPRSAAALAVLERNIIRTQAGLGVPLAVENIAALFDWPDDE
                     YTESEFLTELVERTGVYLLLDIANVYANARNRDRDPLGELLRLPADRVAYCHVAGGHEA
                     GGRYHDTHTDPVPAEVLTLVTEFTARQPVPLMLERDGNYPPAAELLDELDAIAAAAHRP
                     SITGRARAVRA"
     gene            complement(5895..6815)
                     /locus_tag="O3I_RS41175"
                     /old_locus_tag="O3I_041070"
     CDS             complement(5895..6815)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353596.1"
                     /locus_tag="O3I_RS41175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041070"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_014989006.1"
                     /transl_table=11
                     /translation="MGTFDRSAGSDLVVAAGETWGISGSDFLAIYLPFALLAVALGVWM
                     RLRVTRAAPDPGTAHPGAELSVPEVGLLFDDRRAVLTALARLRSADLIDSSAAPTRPLE
                     QADEFRLDPFTLAVHRQIVAGTKPTVAALTVALSGTLAKLRDSMVRRGYFPDADFKRRI
                     WDAGLPTLVVMTVGVVRLVAGMLNNNPVAFLVLALLAQFLCYWFLVGKPRLTPLGKRAR
                     TDARTSSQHLRPANSPAYATYGAESAALAAAVFGGAALLTLDPGLAGAVEPPSSSGGDS
                     GGSGDSGGGGCSGGGGCGGGGGCGG"
     gene            complement(6965..7372)
                     /locus_tag="O3I_RS41180"
                     /old_locus_tag="O3I_041075"
     CDS             complement(6965..7372)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049939.1"
                     /locus_tag="O3I_RS41180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041075"
                     /product="SdpI family protein"
                     /protein_id="WP_014989007.1"
                     /transl_table=11
                     /translation="MFVVALVLFVLGLVALATGALGLTGRLPRNRFVGVHTEAALADDE
                     TFRVANRIAAPTSLAAGALLLAGGLVVLAAGGFAALFVAVGVAVVALFTLGAGANAAAA
                     KAAAIAPPAEVGGCGSACGACSLRDACQPAT"
     gene            7725..10571
                     /gene="leuS"
                     /locus_tag="O3I_RS41185"
                     /old_locus_tag="O3I_041080"
     CDS             7725..10571
                     /EC_number="6.1.1.4"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="leuS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212058.1"
                     /locus_tag="O3I_RS41185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_041080"
                     /product="leucine--tRNA ligase"
                     /protein_id="WP_014989008.1"
                     /transl_table=11
                     /translation="MQDTRATDSDVPEHRYNAALAARIEARWQRTWADRGTFHAPNPVG
                     PLAGDTPADKLFIQDMFPYPSGAGLHVGHPLGYIATDTFARYHRMHGRNVLHALGFDAF
                     GLPAEQYAVQTGAHPRVTTESNIANMQRQLSRLGLGHDSRRSFATTDPAYYRWTQWIFL
                     RIYNSWYDQELDRARPITELEAQFASGARPVPDGKTWDGLSATERGALLDSYRLVYLTD
                     SMVNWCPGLGTVLSNEEVTADGRSERGNFPVFRKRLWQWMMRITAYADRLIDDLDHLDW
                     SDQVKAMQRNWIGRSRGAQVKFDVAGEQIEVFTTRPDTLFGATYVVLAPEHELVDKLTA
                     AAWPDGTDPRWTNGGAANPADAVAAYRKSIVAKSDLERQENKEKTGVFLGVHAVNPANG
                     RAVPVFVADYVLSGYGTGAIMAVPGHDQRDWEFATVLGLPIVEVITGGDLAEAAYSGDG
                     ALVNSGYLDGLSVEEAKATIIARLEADGHGTGTIQYKLRDWLFARQRYWGEPFPIVFDE
                     DGRPHALPESMLPVELPELDDFAPVTFDPDDADSEPSPPLAKATEWVNVELDLGDGPKM
                     YRRDTNVMPNWAGSSWYQLRYADPTNPEAFCAKENEQYWLGPRPAEHGPDDPGGVDLYI
                     GGMEHAVLHLLYARFWQKVLFDLGDVSGREPYRKLFNQGYIQAYAYTDARGAYVPAAEI
                     VERDGKFFWTEPGGTEIEVFQEYGKIGKSLKNAVSPDEMYDLYGADTFRFYEMSMGPLD
                     SSRPWATKDVVGAHRFLQRVWRLVVDEETGAVRVAELDPTPETLRLLHKTIAGVDEDYA
                     ALRDNTAGAKLIELTNHLTKTYPDGAPRAVVEALVLMLAPLSPHIAEEMWERLGHNESL
                     AHGPFPVADPALLVQESVEYPIQVNGKVRSRIQVPADADNAAIEAAALTDEKIAALLNG
                     AAPRKLIVVPGRLINIVA"
     gene            10589..10807
                     /locus_tag="O3I_RS41190"
                     /old_locus_tag="O3I_041085"
     CDS             10589..10807
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS41190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O3I_041085"
                     /product="hypothetical protein"
                     /protein_id="WP_014989009.1"
                     /transl_table=11
                     /translation="MSKTEYRALHFSQANPRGAGQGDVPALLRAIAATLTELGAVSVQD
                     LVLHNEVTAEGDWPSVTVYYTRTDGDD"
ORIGIN
        1 gcaccggact ggacgacgcg ctggccggct gcaccgtggc gagcctcggc gtcgtccccc
       61 cgcatctgtc cgcgatccac gcgacgagca ccgatctggc ccgcaccctg gccgagctgg
      121 ccggcgaatt cgccaccacc gcaattcttt ccgagtcaca cacggccacc gcgctcacac
      181 cgctgcatgt gcccgccgcg accgggcccg ggctgcgcta tcgcgaactc gtcgaaacca
      241 tcgaggcggc actgcccgcg ggcaccgacg tcttcgcgga cgcgggcaat accggcgcgg
      301 cggtggtgca tcagctgcgg gtgccgcgcg gtggccggtt cgccgtggcg ctcggcatgg
      361 gcggcatggg ctacgcgttc ggcgccgcga tcggttccgc gttcgcccgg cgcgacggtg
      421 gccggcgcac cgtggtgatc gcgggtgacg gcgcgttctt catgcacggc atggagatac
      481 acaccgcggt cgagcacgcg ctgccgatca ccttcatcgt gctcaacaac aacgcgcacg
      541 ccatgtgcgt gacccgggag cagctgtact accgggatcg atacagctac aacaggttcc
      601 ggcccgcgca actcggcgac ggcgtcggcg cgatgttccc ggcgctgccc gccgtgtcgg
      661 tgcgcgcgcc cggcgaactt cccgcagcac tgcgcgcctg cctggaaaca cctgggccgt
      721 cgttcctttc gatcgacgtc gatcccgacg aggtcccgcc gttcctcccg ttcctgacga
      781 ccatcgaaca ctccaggagt agaaatgacc gcatcgcgta gcgattcccg cagcagcggc
      841 gtccggccgc ccggcgcgct gcccgcgctc agcgatatcc cggcggaggt cgtcccgggt
      901 gtgctgcgca tcgagaactc cgacaaggac gcgaccaccc cgatcatcat ggacatgctg
      961 cggtcggtct atccgcacga ccagatcttc ggcgaatact gcccggtgca ggcgtatatc
     1021 gccgcaccgc cgcgcgaggt ctacgaatac ctcgcggaca ccaggtcttt ggaggagtgg
     1081 acctacagcc tgcgcggctt cgccgagacc gacgagcccg gcctctggct ggcctacgac
     1141 cggctcggcg acgagaccca gatcttcacg cgcaccgtgg cgcatcccgc ggcgatgacc
     1201 gtggactacc actgcgcctg ggatcagtcc cggcatctgt ggatgatcta cctgctgcgc
     1261 gtgatagatg cccagacggt gttcgacaag ccgggatcgg tggtgctgtg ggtcaattgc
     1321 aaacacccct tctacgacga gaacccctac cccgagaccg cgccgccgag gcggccggtg
     1381 tgggtcggtg acttctggga gatgttctcc gcgggccatc agctggaaat ggacaacctc
     1441 aaagcgattt gcgagtaccg ggcggccaac ggcctgcccg tcaagcccga ctggatgaac
     1501 tgagcccggt atgaattcct ttcccacggt cagcctcgtc gacgtcgccg ggtatctgcc
     1561 cggcgaaccg gtcggcaccg aatacttcac ccagttctcc cgctcggacc ggatggcgaa
     1621 gaacgtgatg ttccggtccc ccacggcccg ccaccacgtg gatcgcgacg agaccgcggt
     1681 ggacatggtc gagcgcgcgg tcgccccgct gatcgaacgg catggggcac aattcctctc
     1741 gcagatcgac gtgttgatca cgcacaccca gctgccgacc aatccggtca tgggctgcgg
     1801 gcccgaggtc gcccggcggc tcggcgcgcg acccggcttc gtctacgacg tgcacaacgg
     1861 cggctgcgcg gccttcgtgc acatgatgtc gatggcgcag atggtgttgc agaccaccga
     1921 cgcgcgcacc gcgctgatcg cggccaccca gaactgcgcc ggcccggtct tcacccagcc
     1981 cgagatccgc aagctggccc aggcgccggt gcccggggac gggtgcggcg tcggcctgct
     2041 ggtcaaagac gacagcgcgc cgatcctgga catcgaatgc cgcacgtacc ccgagttcgc
     2101 cggtgacatg gagttctcca ccaacgggga ccgcaagtac tgggagccgg gcgaggggca
     2161 gggctgcgtg agcttcaccg aatcgaagat caccaaggtg ttcgcgcgcg gtaaccggct
     2221 ggtgccggaa gtggcgctgg ccgtgtgcga tcggatcggg gtgaagggtc gcgacatcga
     2281 caccttcgtc accaatcaac ccaaccgcct gttcctgcgg aactggcacg acgcactgga
     2341 actgcccgcc gaacgacacc cggacacctt cgaccggtgc ggaaacctgt tcgccgcggg
     2401 cattccggtg accctggatg tggagaaccg agcgggccgg ctgcgcaacg gctcggtggt
     2461 cctgctgtcg gccttcgcgc acgcgggtga tttcgcggcc gccgcggcca tccggtgggg
     2521 cgcggccgga tgaacgtccg gttcgtcgag gagaccacgc cgtcacgaca gcggcgcggc
     2581 gccgctacgc cgccgcggtt cgatctggcc ctgagcgaaa acccgttccc gccattgcct
     2641 tcggtgctgc gcgcggtatc cgtcccgctg gcgcaggcga atcggtatcc cgagttcctg
     2701 cccgaacggt tgccccggct gatcgcccgg cacctcggcg tccgagcgga tcagctcgtg
     2761 gtcggcgcgg gcgcgaccgg ggtcgcctgg cagatcatcc agtcgctgac cacgccgggt
     2821 gccggaatgg tctacgggtc accgactttc gacggctacc cgatcatggc ggagatggcc
     2881 gggctcgacc cggtgccggt gccgttggac gccggcggcg atcaggacct gagggcgctg
     2941 gccaaggccg tcgacaggcg caccgggctg gtggtggtgt gccgcccgca caacccgacc
     3001 ggcaccgtgg tcccggccgc agagctggcc gcgttcctgc gcgcggtgcc cgcgcgagta
     3061 ccggtgatcc tggacgaggc ctatgtcgaa ttcctcgacg ccgccgacca tgtcgacccg
     3121 cgcgtcttgc tgagcagaca tgcgaacctg cttgtgctgc gcaccttctc gaaggcgtac
     3181 ggtctggccg ggctgcgcat cggctacgcg ttcggcaggc gcgatctggc ggcccgggtg
     3241 cgcaggctgc aactgccgtt cgggatgagt tcgacggcgg aggcggcggt cgccgcctcc
     3301 tacgccgcgg caccggaact cgccgcacgc accctgcgca tcaccgccgc ccgcgaggaa
     3361 ctgcgcaccg cgttgcggga ccgcggtatc gcggtgccgc gcagccaggc caactttctg
     3421 ttcctgcccg gtcccggtgt ggccaccgcg ctgcgccggg ccggtatcgg tgcgaaggcg
     3481 tatcccgatg ggagcgcgcg catctcggtc ggcgaccccg acgccgaccg ggccgtgttg
     3541 cacgcgctgg ccggactacg ttaagcggcc ggtttcaatt cgctggtttc ccccggatcg
     3601 tcggcctgct tggcccagcg ctgcgacagt gcggcacagg tgatcagctg gatctgatgg
     3661 aacatcatca gcggcagcac gatcaggccc accggctggc tggcgaaaag caccgtcgcc
     3721 atgggcaatc cggtcgcgag gctcttcttg gagccgcaga acacgatcac gatgcggtcg
     3781 gcccgggaga agccggcgaa acggccgatc agcatggtca aggcgatgat gagcgcgagc
     3841 agcacacagc acacgccggc cagcgcgaga atccgccacg gcgagagcat gtgccagatg
     3901 ccctccacca tgcccgcgct gaacgcggcg tagacgacga gcaggatgga gccgcggtcc
     3961 accagcttcg tcggctcggc gtagcggctc aaccagccga tcaccttcgg ccggatcagc
     4021 tgcccgatcg cgaacgggac cagcagttgc agcacgatgt cgccgatcga cgagaagtcc
     4081 acgctggcat ggccggtggt gttcatcagc aggatcacca gcagcggggt gatgaacaca
     4141 ccgagcaggt tcgaggtcgt cgcgctcacg atcgcgcccg gcacattgcc tttcgcgatc
     4201 gaggtgaacg cgatggacga ctgcacagtg gacgggacca ggcagaggaa cagcagaccg
     4261 gtgtacaggt cgtcggtcag cacgctcggc accagcaccc gtgccgccag gcccagcaac
     4321 gggaaggcga cgaaggtgca ggcgagcacc accagatgca accgccagtg cgtgaggccg
     4381 gccagcgcct ccttcggcga gagacgcgtg ccgtacagca ggaagagcaa tgcgatggca
     4441 atcttcgtca gcacgtcgac cacgtccgcc gcggtacctc tggccgggaa aacgctggcc
     4501 agggcgacag aagcgaggat cgacaggatg aatccgtcga tatagaactt gctgaggaat
     4561 cgcacagccc aggctaacgc cgcatcgagc ccctagcgcg cccacaccgt gcgtactttc
     4621 tcacatccca tgaattgcga tatcactccg gacgaagccg aacgttcgtg cttatcgggg
     4681 tcgcggggat cagcggcgcg gccatctctt cgtagtggtg ccgacaggtg ccgggagacc
     4741 ctgagacgcc gcgaaggcgg tggcgtcggc cgcggtgctg gtcttcgatc tgccgtcggc
     4801 ccaggcgagg aaacgctcgc ggaagtcggg gccgcaggcc tgggccagca tcgggaagcg
     4861 gcgggctacc tcgtccgcgc gtttgcggag cagggcgtgc gcggcggccg cgacgtccgc
     4921 gcagtcgaat cccgcgggca ccggggcccc ggccaccagg gcacggacca gagccgcttg
     4981 ctgcgcagcc aattcggcac tcatgcgcgc accgcccggg cacggccggt gatcgacgga
     5041 cggtgcgccg cggcggcgat cgcgtcgagt tcgtccaaga gttcggcggc gggcgggtag
     5101 ttgccgtcgc gctcgagcat caggggcacc ggctgccggg cggtgaattc cgtgacgagg
     5161 gtgagcactt cagccggaac gggatcggtg tgcgtgtcgt gatagcggcc gccggcctcg
     5221 tgaccaccgg cgacgtgaca gtaggcgacc cggtcggcgg gcaggcgcag tagttcgccg
     5281 agcggatcgc ggtcccgatt gcgggcgttg gcatagacat tcgcgatatc gagcagtaga
     5341 tacaccccgg tccgctcgac caactcggtg aggaactccg actcggtgta ctcgtcgtcg
     5401 ggccaatcga acagcgcggc aatgttttcc acggccagcg gtaccccgag ccccgcctgg
     5461 gtccggatga tgttgcgttc gagcacagcc agcgccgcgg ccgaccgcgg caccggcagc
     5521 agatgccccg cctcgacgcc gcccgcgcgc acgaaggcga tgtgctcgct caccagcgga
     5581 gcggccagcg ccgccgcagc ggtggccagg tgcgcgatcc gctgctcgcc gaccggctcg
     5641 gccccgccca gcgacagcga aataccatgc ggcacaacag cgacaccgcg atcgatcagt
     5701 gcggtcagct cggcgggcgg cgccgccgtc agcccgccgc gcagccggga ccgccgccgc
     5761 aacggcagcg actccgcgat cacctcggcg aaaccgagcc cgtccagttc ggcgatgacg
     5821 ccgcagatct cgggccgcca gccgatgccg agtgccgccg ccggcaacag tccattcatc
     5881 gggcggccgg accgtcaccc accacagccg ccgcccccgc cacagccacc gccgccactg
     5941 cagccgccgc caccgctgtc cccgctcccg ccgctgtcac caccggacga gctcggcggt
     6001 tcgaccgcgc cggccagccc gggatcgagc gtcaacagtg cggccccacc gaagaccgcc
     6061 gccgcgagcg cggcgctttc cgccccgtag gtggcgtagg cgggcgagtt ggccggccgc
     6121 agatgctgac tgctggtcct cgcgtcggta cgggcccgct tgccgagcgg agtcagccgc
     6181 ggcttgccca ccaggaacca gtagcagagg aactgcgcca ggagagcgag gacgaggaac
     6241 gcgaccgggt tgttgttgag catgcccgcg acgagccgca ccacaccgac cgtcatgacg
     6301 accagcgtcg gcaggcccgc gtcccagatc cgccgcttga agtccgcgtc cggaaagtag
     6361 ccgcgccgca ccatggaatc gcgcagcttg gcgagcgtgc cggacaacgc gacggtgagc
     6421 gcggccaccg tcggcttggt cccggcgacg atctgccggt gcaccgccaa ggtgaacggg
     6481 tccaaccgga attcgtccgc ctgctccagc ggccgggtcg gggcggcgct ggaatcgatg
     6541 aggtcggcgc tgcgcagccg ggccagcgcg gtgagcaccg cgcgccggtc gtcgaacagc
     6601 aggccgacct cgggcacgga cagttcggcg ccggggtgcg cggtgcccgg gtccggcgcg
     6661 gcgcgcgtca cccgtagccg catccacacc ccgagcgcca ccgcgagcag cgcgaacggc
     6721 agatagatcg ccaagaaatc ggatccggag atgccccacg tctcgcctgc cgcgacgacg
     6781 agatcggagc ccgcgctccg gtcgaacgta cccatatcca ccccggagtc gtgccagtag
     6841 tttccccagt atgggaccgc cccacaccag tgccaaccgg attcggcgag gtgatcggat
     6901 cgaaacctgg gcgaataggg ttacgagcgg gccggtggaa tggttcgacc ggatgaaaca
     6961 ggccctaagt cgcgggctgg caggcgtcgc gcagcgagca ggcgccgcac gcgctgccgc
     7021 agccgccgac ctcggcgggc ggggcgatcg ccgcggcctt ggccgcggcc gcgttggcgc
     7081 ccgcccccag ggtgaacagc gcgacgacgg cgacacctac ggcgacgaac agcgccgcga
     7141 aaccacccgc ggcgagcacg acgagaccac ccgccagcag cagcgcaccc gcggccaggg
     7201 aggtgggggc ggcgatgcga ttcgcgacgc ggaaggtctc gtcgtcggcg agggcggcct
     7261 cggtgtgcac cccgacgaag cggttgcgcg gcagccggcc ggtcagcccg agcgctccgg
     7321 tcgcgagggc gacgaggccg agcacgaaca agacgagagc gacgacgaac acccatgcag
     7381 gctatcgcat cggtgtcgac ggaccgccga cagctcccgc gcaaccctgc agcacccggc
     7441 gcgtcaccac tgcggacaag cgctttccac actgcgccgg gacagctcgc ggacctagca
     7501 accaaacgca ctgttgggtc cgccgagcgg agaatgccaa ctctgcggta tcgcgaaacc
     7561 ggtgtgccgc agccgcatac cgacgggtgg gtaacccggg aacagagatc ggcacgggcg
     7621 cgcgaacgga gtcagcgcag cccgattttc agtactgcgg tgtgcacgtt ctaccctgat
     7681 agacgaagtt tgtcggggat cggcgataac aagaaggcag gaccgtgcag gacactcgtg
     7741 cgaccgatag cgatgtaccc gagcatcggt acaacgccgc gcttgccgcc cgtatcgaag
     7801 cgcgttggca gcggacgtgg gccgaccgcg ggaccttcca tgcgccgaac ccggtcggtc
     7861 cgctagcggg cgacaccccg gcggacaagc ttttcatcca ggacatgttc ccgtacccgt
     7921 cgggggcggg cctgcacgtc gggcatccgc tcggctacat cgccaccgac acgttcgcgc
     7981 ggtatcaccg gatgcacggc cgcaacgtgc tgcacgcgct cggcttcgat gccttcggcc
     8041 tgcccgccga gcagtacgcg gtacagaccg gcgcgcaccc gcgggtgacc accgagtcga
     8101 acatcgccaa catgcagcgc cagctgagca gactcggact cggccacgac agcaggcgct
     8161 cgttcgcgac caccgatccg gcgtactacc gctggaccca gtggatcttc ctgcgcatct
     8221 acaactcctg gtacgaccag gaactcgacc gcgcgcgccc gatcaccgag ctcgaagctc
     8281 aattcgccag tggcgcacgg ccggtcccgg acggcaagac gtgggacggg ctctcggcca
     8341 ccgagcgcgg cgcgctgctg gactcgtatc gcctggtcta cctgaccgat tcgatggtca
     8401 actggtgccc cggcctcggc acggtgctgt ccaacgagga ggtcaccgcc gacggccgca
     8461 gcgagcgcgg caacttcccc gtgttccgta agcgcctgtg gcagtggatg atgcggatca
     8521 ccgcctacgc cgaccggctg atcgacgacc tggaccacct ggactggtcg gatcaggtga
     8581 aggcgatgca gcgcaactgg atcggccgct cgcgcggcgc ccaggtgaag ttcgacgtcg
     8641 cgggcgagca gatcgaagtg ttcaccaccc ggcccgacac cctgttcggc gcgacctacg
     8701 tggtgctcgc accggaacac gaactggtgg acaagctgac cgcggcggcg tggccggacg
     8761 gcaccgatcc gcgctggacc aacggtggcg ccgcgaatcc cgcggatgcc gttgcggcgt
     8821 accgcaagtc gatcgtggcg aagtcggatc tggagcgcca ggagaacaag gagaagaccg
     8881 gcgtcttcct cggcgtccat gccgtgaacc cggccaacgg ccgagccgtg ccggtcttcg
     8941 tcgccgacta cgtgctgagc ggctacggca ccggcgcgat catggcggtg cccggccacg
     9001 accagcgcga ctgggaattc gccaccgtgc tcggcctgcc gatcgtggag gtcatcaccg
     9061 gcggcgacct cgccgaggcc gcgtactccg gtgacggcgc gctggtgaac tccggctatc
     9121 tcgacggact gtccgtcgag gaggccaagg cgaccatcat cgcccggctg gaagccgacg
     9181 gtcacggcac cggcaccatc cagtacaagc tgcgcgactg gctgttcgcc cgccagcgtt
     9241 actggggcga gcccttcccg atcgtcttcg acgaggacgg caggccgcac gcgctgccgg
     9301 aatcgatgct gcccgtggag cttccggagc tggacgattt cgccccggtc accttcgacc
     9361 cggacgacgc cgactccgag ccgtcaccgc cgctggccaa ggccaccgag tgggtcaacg
     9421 tcgaactgga cctgggcgac ggcccgaaga tgtaccggcg cgacaccaac gtcatgccga
     9481 actgggcggg cagctcgtgg tatcagctgc gctacgccga cccgaccaac ccggaagcgt
     9541 tctgcgccaa ggagaacgag cagtactggc tcggcccgcg tccggccgaa cacggcccgg
     9601 acgatccggg cggtgtcgat ctgtacatcg gcggcatgga gcacgcggtg ctgcacctgc
     9661 tgtacgcccg gttctggcag aaggtgctgt tcgatctggg tgatgtcagc ggccgggaac
     9721 cgtaccgaaa gctgttcaac cagggctaca tccaggccta cgcctacacc gacgcccgcg
     9781 gcgcgtacgt gcccgcggcc gagatcgtcg agcgggacgg caagttcttc tggaccgaac
     9841 cgggcggcac cgagatcgag gtgttccagg agtacggcaa gatcggcaag tcgctgaaga
     9901 acgcggtctc gccggacgag atgtacgacc tctacggcgc ggacaccttc cgcttctacg
     9961 agatgtcgat gggcccgctg gacagctcgc ggccgtgggc gaccaaggac gtcgtgggcg
    10021 cgcaccggtt cctgcagcgg gtgtggcggc tggtggtgga cgaggagacc ggcgcggtgc
    10081 gcgtcgccga actcgatccc acgccggaaa cgctgcgcct gctgcacaag accatcgccg
    10141 gtgtcgacga ggactacgcc gcgctgcgcg acaacaccgc gggcgccaag ctgatcgagc
    10201 tgacgaacca tctcaccaag acctatcccg acggcgcgcc gcgcgccgtg gtggaggcgc
    10261 tggtgctgat gctggcgccg ctgtccccgc atatcgccga ggagatgtgg gaacgcctgg
    10321 gacacaacga gtccctggcg cacggcccgt tcccggtggc cgatcccgcg ctgctggtgc
    10381 aggagtccgt cgagtacccg attcaggtga acggcaaggt ccgcagccga atccaggtcc
    10441 ccgccgacgc cgacaacgcc gcgatcgagg ccgccgccct caccgacgaa aagatcgcgg
    10501 ccctcctcaa cggcgccgcc ccccgcaaac tgatcgtcgt ccccggccgc ctgatcaaca
    10561 tcgtcgcctg acccacccga gtagccctgt gtcgaagacc gaataccgtg cgctgcattt
    10621 ctcgcaggcg aatccgcggg gggctgggca gggggacgtg cctgcgctgt tgcgggcgat
    10681 cgccgcgacg ctgacggaac tcggcgcggt cagcgtgcag gatctggtgc tgcacaacga
    10741 agttaccgcc gaaggtgact ggccgtcggt cacggtctac tacaccagga ccgacggcga
    10801 cgactgatcg acggcgacgg tgaccggccc gtgcgcgaac cggtttcggc cccttccttt
    10861 ccggcagacg gaacgggaa
//
