LOCUS       NC_018681              21557 bp    DNA     circular CON 21-MAR-2024
DEFINITION  Nocardia brasiliensis ATCC 700358, complete sequence.
ACCESSION   NC_018681
VERSION     NC_018681.1
KEYWORDS    RefSeq.
SOURCE      Nocardia brasiliensis ATCC 700358
  ORGANISM  Nocardia brasiliensis ATCC 700358
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Complete genome sequence of Nocardia brasiliensis HUJEG-1
  JOURNAL   J Bacteriol 194 (10), 2761-2762 (2012)
   PUBMED   22535940
REFERENCE   2  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
REFERENCE   3  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000250675.2-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 01:53:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,551
            CDSs (total)                      :: 8,489
            Genes (coding)                    :: 8,417
            CDSs (with protein)               :: 8,417
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 13 of 72
            Pseudo Genes (incomplete)         :: 63 of 72
            Pseudo Genes (internal stop)      :: 3 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:09:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8570826
            Orig. end                         :: 8592383
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003876.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21557
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ranthipeptide"
                     /region_number="50"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /tool="antismash"
     cand_cluster    1..21557
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /kind="single"
                     /product="ranthipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21557
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11557](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11557)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     gene            complement(113..1243)
                     /locus_tag="O3I_RS38210"
                     /old_locus_tag="O3I_038070"
     CDS             complement(113..1243)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049485.1"
                     /locus_tag="O3I_RS38210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038070"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_014988410.1"
                     /transl_table=11
                     /translation="MRFAPLVAAFAAAALLAGCSSESSPGPEQPPSDNRIREVQENIDK
                     IVAAGGVGAIATVTGDGRTAVLTSGESDTATHAKMPAEPLQRTRIGSITKTFTSAVILQ
                     LVAEGKVRLDEPIDTYLPGLLTGPGIDGRAITVRQVLRHQSGLPELTKDPRVDEYRAAL
                     ENRTMTAAEEIAIALTMPADFAPGARYEYSNTNYIVAGMLIEKVTGAKYVEELEWRILI
                     PLGLSDTYLPGPGERDIRGPHPQGYDEIDGVVTEVSRIEPSVPWAAGAMVSTGTDLNRF
                     FTALLAGKVVPAAELQQMRAGVPMGDETIDMSYGLGVGSIRLSCGAEYVGHSGGIYGFL
                     TLSGATPDGRAVTYTFNKAPKSEPDFIDLLSDALCP"
     misc_feature    1214..1223
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     gene            1387..2208
                     /locus_tag="O3I_RS38215"
                     /old_locus_tag="O3I_038075"
     CDS             1387..2208
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /locus_tag="O3I_RS38215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038075"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_014988411.1"
                     /transl_table=11
                     /translation="MTTIQQPTGDNDRFERGLALLKEIGGPDGPAVLESLGDIAPDLGR
                     ITVEFGYGDILSRPGLTLRERELTTVGALAALGHAAPQLRWHINGALNVGCTRTEIVEV
                     LIHASVYAGFPAALNGLTAAKEVFAARTDLPGEPAPEPAPAGARFERGTAALSEIDGEA
                     GEQVVAALQDIAPDFARLLVEFSFGDIYARPGLDLKTREMVTVAMCTALGVAPQLGVHI
                     HGLLNVGGSEQEVVEVLIQMAGYAGFPAALNGIAVARTVFAERRSHRNADA"
     gene            2260..2955
                     /locus_tag="O3I_RS38220"
                     /old_locus_tag="O3I_038080"
     CDS             2260..2955
                     /GO_function="GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662984.1"
                     /locus_tag="O3I_RS38220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038080"
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="WP_014988412.1"
                     /transl_table=11
                     /translation="MAKILFVLTGADHWTLADGTLHPTGFWAEEFAVPYEAATAAGHEI
                     VVATPGGLVPPVDKGSLAAEYNGGQDIADKRAETLANAVELQRPADLAQVDLADYDAVY
                     YPGGHGPMEDLAVNRESGELLTAALRSGKPLAVVCHGPAALLAATNADGTNAFTGYALT
                     GFTNAEERQGGLADKAKWLLQDRLVELGADFQEGEPWAPKVVVDRNLITGQNPASSAPV
                     AAELLKKLA"
     misc_feature    3001..3013
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     gene            3076..3948
                     /locus_tag="O3I_RS38225"
                     /old_locus_tag="O3I_038085"
     CDS             3076..3948
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011206856.1"
                     /locus_tag="O3I_RS38225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038085"
                     /product="aldo/keto reductase"
                     /protein_id="WP_014988413.1"
                     /transl_table=11
                     /translation="MSTDLNARPAAASGTFAIGADLPVHRLGFGAMQLTGPGVWGDPAD
                     PDEAVRVLRRVVELGVTFIDTADSYGPFVSEQLIRKALHPYPDDLVIATKGGFTRSGPG
                     DWRAVGRPEYLRQQTELSLRHLGLDRIDLYQLHRIDPQVPLADQLGELALLQQEGKIRH
                     IGLSQVSVAQLAQAREIATIVSVQNLYNLANRDDEDVLDYAERENIAFIPWYPIATGKL
                     AAPGGPLDAIAAEHGASPAQLALAWLLRRSPVLLPIPGTSSVAHAEENTEAARITLTDK
                     EFESLAAAV"
     gene            4343..5689
                     /locus_tag="O3I_RS38230"
                     /old_locus_tag="O3I_038090"
     CDS             4343..5689
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS38230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O3I_038090"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_014988414.1"
                     /transl_table=11
                     /translation="MVEIDWTGEDVRALRAAMRRSRYEFARLVGVTPRTVVLWENGRTN
                     RMHAASQRLLDKVVSDADPAVVARFERTVTAGRGSGFDLADSDPAPDPHTQLGRDIGLD
                     KEVEDDDVRRRELLACIGAGLTGRAAELIASEPEKMLATLDAGSMSERRLGLLEHSAEE
                     LGKRVVHLPPHELLHPTLEQFRTVRGALAQRQATHHQVRLVRTAGRLANVVGEILFNEG
                     HFGQARMWYGTAIHAAQDIGDRYSEDIALAGLAYLPTYSDKPKEVLNLLGGRLDDNPAH
                     GPAAAWLWGMAARAHASLGNADQFTRCIEKSARALEDIDQQELTVGIFSYRPEKLAFYQ
                     AIGNARLGRAGETAKAAARALTLYDPRETMEPALVRFEHATALLTAGEIDEACSIATQA
                     ITGNNTYIGLTVTKRAKQFDTALSGFRGRAVTGWRQHARSVLEASRTRA"
     misc_feature    4691..4693
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5707..5868)
                     /locus_tag="O3I_RS45840"
     CDS             complement(5707..5868)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS45840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167829202.1"
                     /transl_table=11
                     /translation="MTNQRITPTLPETLIAAARRWESGRPTPAQTQQISGWLTGVMHRA
                     RPQDATNS"
     gene            complement(5991..6209)
                     /locus_tag="O3I_RS38235"
                     /old_locus_tag="O3I_038095"
     CDS             complement(5991..6209)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1009: mbtH-like protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353044.1"
                     /locus_tag="O3I_RS38235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038095"
                     /product="MbtH family protein"
                     /protein_id="WP_014988415.1"
                     /transl_table=11
                     /translation="MNNPFDNDDAQFYVLINADGEHSLWPIFAAVPGGWTIAYGAAARK
                     ACLHYVETHWTDMRPKSLIEAGSNETS"
     misc_feature    complement(6138..6140)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6820..6833)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.04"
                     /tool="antismash"
     gene            complement(6879..8342)
                     /gene="mftG"
                     /locus_tag="O3I_RS38240"
                     /old_locus_tag="O3I_038100"
     CDS             complement(6879..8342)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene="mftG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353048.1"
                     /locus_tag="O3I_RS38240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038100"
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="WP_014988416.1"
                     /transl_table=11
                     /translation="MTDTLIVGGGTAGCVLAARLSEHPDHTVRLLEAGPVWRAPAEVPA
                     RLRDATRLPVEADAPWLWRYPIALAAADSAEQTGNIVRGRAIGGSSAVNGSYFVRAGPA
                     DFAAWSQVLNGANTWSFDAVLSAYRRSEHDLDFGDRRWHGRSGPIPVRRTADPIPLSEE
                     FANACVVAGFPLLSDLNAPGATDGVGRVPCNIADGVRTSTALAYLLPALNRPNLTVHGD
                     TSALRIVFRGTRAIGVDVLRAGRTETVWADRIVLSAGAIETAALLLRSGVGDAEQLRAS
                     GIPVVHPAPVGAWCTDHPEIGLEYRMPAPARAAVALEYALAVDDLEFRPYTVAFTPGTY
                     RLGVALMRPESAGTVRLRAPDPATPPDIRLGYLRAERDRARLRTAVDLAMDLLHGMSAQ
                     PRTDPMPRTPAQADAWLRANLATSQHLSGTCRMGPPDDERAVVDERCRVHGVTGLSIVD
                     LSVVPVPLSRGPQATVVMIAEHAATHLTP"
     gene            complement(8370..9770)
                     /gene="mftF"
                     /locus_tag="O3I_RS38245"
                     /old_locus_tag="O3I_038105"
     CDS             complement(8370..9770)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889869.1"
                     /locus_tag="O3I_RS38245"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038105"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_041563134.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.4e-29,
                     bitscore: 100.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHDRLPDGFGVRIDPRVRAYSGGRVLIGGSPARLLRLAPEAAEM
                     IGDGYLEVTGPKSATVARRLLDSGVANPRPRLLPSPEDVTVIVPLYNNPDGLARLLAAL
                     RGHNVIVVDDGSDQPVHIPRDRGTRCRVTVLRHDQRQGPAAARNAGLKAATTDFVAFLD
                     SDVVPRSGWLEVMLGHFSDPEVALVAPRIVALDPESNALARYEHTRSSLDLGRREAAVQ
                     SHGLVSYVPSAALLVRRRALLAQDGFDESMQVAEDVDLCWRLEQAGWRLRYEPAAHVAH
                     DHRVSFRAWFGRKMFYGTGAAPLGERHPGMVSPLSLPSWTMLAAFLFATKSKWGLLGGL
                     VTLATALVRLRRVFAELDNPTRIAAIYLSRGFFAGLWRLASAMCRHYWPVTLLAMLASR
                     RIRRIAITMAVADGLADWFTHRDAGGLDPVRYVAYKRLDDIAYGTGLWVGAFRARSPES
                     LKPTAPSH"
     misc_feature    9974..9990
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            complement(10001..11311)
                     /gene="mftC"
                     /locus_tag="O3I_RS38250"
                     /old_locus_tag="O3I_038110"
     CDS             complement(10001..11311)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553544.1"
                     /locus_tag="O3I_RS38250"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="O3I_038110"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_237748431.1"
                     /sec_met_domain="PTHR43273 (E-value: 4.9e-36, bitscore:
                     123.4, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 9.6e-25, bitscore: 86.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.2e-07, bitscore: 30.4,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 4.2e-13, bitscore:
                     48.4, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIIEAPAAATRAPAAAAAVSAAPSTAGVVPPSDAAVPLVDRFESG
                     LAAPICLTWELTYACNLSCVHCLSSSGRRDPRELSTEQCKALIDEFERMQVFYVNIGGG
                     EPTVRPDFWELVDYATAHHVGVKFSTNGVRITPEVAARLAASDYVDVQISLDGADAAVN
                     DAVRGPGSYAMAIRALENLAAAGFRDAKLSVVATRHNIGQLDEFRAIAEKYGATLRLTR
                     LRPSGRGADVWDDLHPTQEQQRVLYDWLLRNGEGVLTGDSFFHLSAFGQALPGLNLCGA
                     GRVVCLVDPLGDVYACPFAIHDRFHAGNVVADGGFRTVWQESELFAELRAPSGGGACAS
                     CSHYDSCRGGCMAAKFFTGLPADGPDPECVQGYGEAALADPARLIPRSSVDHSRRATPR
                     TGARKPAPVPLTLSVTRPSGFSPERRPARACDESPLA"
     gene            complement(<11312..11557)
                     /gene="mftB"
                     /locus_tag="O3I_RS46730"
                     /pseudo=""
     CDS             complement(<11312..11557)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.2"
                     /locus_tag="O3I_RS46730"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /pseudo=""
                     /sec_met_domain="Mycofactocin_RRE (E-value: 5.4e-28,
                     bitscore: 96.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDLDDRWQLHPRVALRPEPFGALLYHFGTRKLSFLKSPRIVEIV
                     RSLPAHQSARAALGAAGISAENSAQYVKALTQLVDSE"
     aSDomain        complement(11315..11545)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_O3I_RS46730_Mycofactocin_RRE.1"
                     /evalue="1.80E-30"
                     /identifier="RREFam016.1"
                     /label="O3I_RS46730"
                     /locus_tag="O3I_RS46730"
                     /protein_end="81"
                     /protein_start="4"
                     /score="96.0"
                     /tool="antismash"
                     /translation="DDRWQLHPRVALRPEPFGALLYHFGTRKLSFLKSPRIVEIVRSLP
                     AHQSARAALGAAGISAENSAQYVKALTQLVDS"
     gene            complement(11572..>11637)
                     /gene="mftA"
                     /locus_tag="O3I_RS46970"
                     /pseudo=""
     CDS             complement(11572..>11637)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019663502.1"
                     /locus_tag="O3I_RS46970"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis.;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="mycofactocin precursor MftA"
                     /pseudo=""
                     /transl_table=11
                     /translation="APLVEESLIDEVSIDGMCGVY"
     misc_feature    complement(11678..11693)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.07"
                     /tool="antismash"
     gene            11998..12636
                     /gene="mftR2"
                     /locus_tag="O3I_RS38255"
                     /old_locus_tag="O3I_038120"
     CDS             11998..12636
                     /codon_start=1
                     /gene="mftR2"
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211601.1"
                     /locus_tag="O3I_RS38255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038120"
                     /product="mycofactocin system transcriptional regulator
                     MftR2"
                     /protein_id="WP_228833046.1"
                     /transl_table=11
                     /translation="MGVGRMQASGGGNTLSESEIVEAALRVVREDGVEKLSMRRLSREL
                     GVSPMAPYYYVADKRELLDLVATAALTGVRTPPAESGPWQIRLRDLIDQIDEKLRKHPG
                     LGDVLIEQMLGKQLDLIAAIMEILFEAGFNDRNVLAAYATIHTYLFGRSRVNPRDRAPL
                     SDVALPNAVERATKHMADLRGKYAYDFGMEVLIAGLEAQLVRQPTSDVR"
     gene            12699..13808
                     /locus_tag="O3I_RS38260"
                     /old_locus_tag="O3I_038125"
     CDS             12699..13808
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1094: ferredoxin"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889848.1"
                     /locus_tag="O3I_RS38260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038125"
                     /product="ferredoxin--NADP reductase"
                     /protein_id="WP_051066840.1"
                     /transl_table=11
                     /translation="MDAASRSDPVGGGGRATGGPTVEVPHGSRSVVLRVAAVITETPDT
                     CSLVFDVPDELAERFRYHPGQFLTLRIPSDLTGSVARCYSLASSPFTDDHPKVTVKRTA
                     GGYGSNWVCDNVKAGDSLELLPPSGVFTPKDLDADLLLFGAGSGITPVMSILKSALARG
                     SGRIVLVYANRDHESVIFADELRDLADKHPQRLTVIHWLEHLQGLPTADALATLASPYQ
                     SFEAFMCGPKPFMDRVHDALAQLGVPRSRTHAEVFNSLAGDPFADQAPVEVSDAEAAEA
                     ATVEVELDGEVHELSWPRKQTLVDIMLAKGLDVPYSCQEGECGSCACTVLEGKVEMDNA
                     EILDPEDIENGYILGCQAHPVTDHLKIQF"
     gene            complement(13813..14553)
                     /locus_tag="O3I_RS38265"
                     /old_locus_tag="O3I_038130"
     CDS             complement(13813..14553)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728380.1"
                     /locus_tag="O3I_RS38265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038130"
                     /product="DNA alkylation repair protein"
                     /protein_id="WP_014988422.1"
                     /transl_table=11
                     /translation="MTEGAQTAAAVQAALRAIADPADAIHLQRFFKTGPGEYGEGDVFV
                     GVRVPATRGVAKRFAALPLDEIDLLLDSSVHEDRLAGLVVLNARFAKASKPRTFDDAAR
                     AEMVDRYLAAVRRGRVNNWDLVDVSAENIIGPWLLDKPRELLFELAAADSLWERRVALL
                     STFAFIKSGDATTTFALSERLLDDRRDLIQKALGWMLREVGKRIDQRLLTGFLDQHAAD
                     LGRTALSYATEHLDPAVRAHYRAR"
     gene            complement(14579..15340)
                     /locus_tag="O3I_RS38270"
                     /old_locus_tag="O3I_038135"
     CDS             complement(14579..15340)
                     /GO_function="GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA]"
                     /GO_process="GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049346.1"
                     /locus_tag="O3I_RS38270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038135"
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="WP_014988423.1"
                     /transl_table=11
                     /translation="MIVALIDSGLGLLPTSAWLRKLRPDVDLLLQLDPDGAPWGPKPEQ
                     WTVDRVVRAAGMSIELGAEVIVIPCNTASVTALEHVRAEVGPDVPVIGTVPAIKPAAAV
                     CRSVAVWATAATTASRYQADLIAKFGGNADVVGVACHGLADAIDRGDLVGARESIARAV
                     AETPDDVEGVVLGCTHYPLVIDAIVAALPDGVRLFDSAQAVAAQTIRRMDALGRPTPGN
                     GAVLVRNSGRPGELPASAAAFESGRILGAQG"
     gene            complement(15467..16678)
                     /locus_tag="O3I_RS38275"
                     /old_locus_tag="O3I_038140"
     CDS             complement(15467..16678)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1175: pyridine nucleotide-disulfide oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211605.1"
                     /locus_tag="O3I_RS38275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038140"
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="WP_141692078.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 8.5e-57, bitscore:
                     191.6, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPSATDTAPIVIVGAGLAGLRTAEELRRTGYEGALVLLGDEPRL
                     PYDRPPLSKQFVRGETDDTTLRPDEFYTEKQIDVRLGVEAVGLDTAARQVRLADGTALD
                     YAQLIIATGLRPRRLPGLPDLAGVHVLRGHADALALRSELATATTALVIGAGFIGCELA
                     ASFRARGVAVVLVEPQPAPLAGVLGERVGELVARMHRAEGVDLRCGVGLHSLLAAGDRV
                     RGAILSDGTEVAADLVVIGVGSRPVTEWLAESGIALADPAAGGGVLADEVGRTSAERVW
                     AVGDVAAWQQEAGHRKRIEHWTNAGEQARLVACALLGAAAPTAARVPYFWSDQYDMKIQ
                     ALGTPAATDDVDVAVDDGRKFLAYYSRDGRLTGVVGAGTTAQVMKTRAKIAAGAPVSEL
                     LTPS"
     gene            16833..17192
                     /locus_tag="O3I_RS38280"
                     /old_locus_tag="O3I_038145"
     CDS             16833..17192
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353056.1"
                     /locus_tag="O3I_RS38280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038145"
                     /product="SPW repeat protein"
                     /protein_id="WP_014988425.1"
                     /transl_table=11
                     /translation="MFTESRAQDFLAVVLGVFAALSPLWLETTDKAMWSLIVLGVLIAL
                     TGLAQMYRPALGANADYAMGLFGVLLFISPWAMDFSDYRGASWTAWVVGVVTAVVAVAA
                     LPTVSGRLHNMAPHH"
     gene            17198..17854
                     /locus_tag="O3I_RS38285"
                     /old_locus_tag="O3I_038150"
     CDS             17198..17854
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889844.1"
                     /locus_tag="O3I_RS38285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038150"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_086006317.1"
                     /transl_table=11
                     /translation="MPKPGRRATRRPGKEPAAGRRRSAKVDGDARQLILDSAEQLFAEQ
                     GFDATATAAIAGAAGVPKGLVFYYFPTKDSILSTLMAERVPAHPIEDIGTVVAPGDPAA
                     SLVALDAALNLRDHHSSVLRVIMWREADTHPDVRRQLRRLRDQLLDVTARVLQASAPTP
                     VRPGTLRACAAAWVSAMFAIASTDRLHALDGVPLPTADELLNVAQVVAAGMTQLG"
     gene            17956..18903
                     /locus_tag="O3I_RS38290"
                     /old_locus_tag="O3I_038155"
     CDS             17956..18903
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS38290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O3I_038155"
                     /product="hypothetical protein"
                     /protein_id="WP_014988427.1"
                     /transl_table=11
                     /translation="MDAPQLISRQQALAAGISDSELRRRCLDGSWWRVRAGRYVNAPEP
                     GLTPAGRHLVLALATCEAMPEDAVASHCSAVVLHGVPCWSIPLDRVHLTRNRINGGRVT
                     NQLVVHSAQLTPDEITIVNGLQVTTAPRTVVDIARSASFEQAVVIGDNALHQGLTTADE
                     LQECLSRARHRRGARRAAHTIDFLDGRSGSIGESRSRVAFREGGLPAPELQARVFTDDD
                     VCVGRVGFLFPELGVIGEFGGKMPCQSALRTPGQGVIAEKNRADRLRALGWMVVRWTWQ
                     DLTDPAHLARRIRAAGRAAAYGRRAGHWTPTPRT"
     misc_feature    18706..18708
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(19474..20289)
                     /locus_tag="O3I_RS38295"
                     /old_locus_tag="O3I_038160"
     CDS             complement(19474..20289)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211608.1"
                     /locus_tag="O3I_RS38295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038160"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_014988428.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.8e-09, bitscore:
                     37.5, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKSTLAAELDVTEGALRHGMPYLAMGAGRPLVFLRWFTPDHANP
                     RGWMRDSEIKMLAPLARHFRVCAVSRAPGMAAGTTMADIAAEHADALRAEFGEPVDVVG
                     VSSGGSVALQLAVDHPAAVRRLIVASSGYRLDPLAKESQIRYGEAAFAGRRALHHMAST
                     TVSSPILARVAGAAMWLIDPLARPKNPADTLAFLRAEDDFDLSDRLGEILVPTLVLGGE
                     RDAGYSTENFRRTADGIPDSRLIIYPGTSHTGVTRHPRFAADIVEFLSA"
     misc_feature    complement(20456..20469)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.88"
                     /tool="antismash"
     gene            20673..21443
                     /locus_tag="O3I_RS38300"
                     /old_locus_tag="O3I_038165"
     CDS             20673..21443
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083350.1"
                     /locus_tag="O3I_RS38300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_038165"
                     /product="oxidoreductase"
                     /protein_id="WP_014988429.1"
                     /sec_met_domain="adh_short_C2 (E-value: 3.6e-30, bitscore:
                     104.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVENGSGTKELAGKRALVTGGTRGIGAAVVRQLLDAGAEVLTT
                     ARSETSTVPEGAAFVAADVRTRAGAETLAAAAQGMFGGVDILVHNAGGASPYQGALAIP
                     DQVWQDALDLNFLASVRLDSLVAPGMRDRRSGTIVHISSAAVPTVAPPFLHYTTAKAAL
                     ENYSRGLAAELAPFGIRVNTVSPGKTATPGGEATREQWAHLDSGPGQNNTTPPLGRDGR
                     PADIAHAVLFLVSPRASWLTGSSIVVDGGEFPRG"
ORIGIN
        1 atggccgacg tgcggcaccg aatccacaag cacaagaacc tgatctgagc gtgacaccgt
       61 cgtccgggcg gcgcaccacc cggacgacgg agacccctcg atcagacagg actcacgggc
      121 agagcgcgtc gctgagcagg tcgatgaaat cgggttcgga cttcggcgct ttgttgaagg
      181 tgtaagtcac ggcccggccg tccggggtcg cgccggacag cgtcaggaag ccgtagatac
      241 caccgctgtg cccgacatat tctgcgccgc aagacaatcg gatcgatccg acgccgagcc
      301 cgtaggacat gtcgatggtc tcgtcgccca tcggcacacc ggcccgcatc tgctgcaatt
      361 cagcggcggg caccactttc ccggccagca atgcggtgaa gaagcggttc aggtcggtac
      421 cggtggacac cattgcgccg gccgcccacg gcaccgacgg ttcgatgcgg gacacctcgg
      481 tgaccacgcc gtcgatctcg tcgtaaccct gcgggtgcgg accgcgaata tcccgctcac
      541 cgggaccggg caggtaggtg tccgacagcc cgagcggaat caggatccgc cactcgagtt
      601 cctcgacata ctttgcgccc gtgacttttt cgatgagcat cccggcgacg atgtagttgg
      661 tgttgctgta ctcgtagcgg gcgccgggcg cgaaatcggc gggcatggtc agcgcgatcg
      721 cgatctcctc ggccgcggtc atggtgcggt tctccagcgc cgcccggtac tcgtcgacgc
      781 ggggatcctt ggtgagttcg ggcagcccgc tctgatgacg caagacctgg cgtaccgtga
      841 tcgcccgccc atcgatgccc ggtccggtca gcaggcccgg caggtaggtg tcgatcggct
      901 cgtccagccg gaccttgccc tccgcgacca gttgcagaat caccgcactg gtaaaggttt
      961 tcgtgatact gccgatccgg gtgcgctgca gcggctcggc gggcatcttc gcgtgcgtcg
     1021 cggtgtcgga ctcgccactg gtgagtaccg cggtcctacc gtcaccggtg acggtcgcga
     1081 tcgcgccgac gccacccgcc gcgacgattt tgtcgatgtt ctcctgcacc tcacgaatcc
     1141 tgttgtccga cggcggttgc tccgggcccg gcgacgattc cgacgagcac ccggcaagca
     1201 gtgcggcagc ggcgaaggcg gcgacgagcg gtgcgaagcg catacccgca acggtaggta
     1261 agtcatcggt ggacgcacat ccgggacgaa cccggagatc gcccctgagg cgctgtcccc
     1321 ttcgaggatg agaaagcgcc cgcgagggct tgcgttggag tgcactccac cttttacagt
     1381 cctcccatga ccacgatcca acagcccacc ggggacaacg accgtttcga gcgcggcttg
     1441 gcactgctca aagagatcgg cggacccgac ggtcccgcgg tgctggaaag tctgggcgat
     1501 atcgcacccg acctgggccg gatcaccgtg gagttcggct acggcgatat cctgtcccgc
     1561 cccggcctga ccctgcgcga acgcgagctg accacggtcg gcgccctggc cgcactcggg
     1621 cacgccgcgc cgcaactgcg ctggcatatc aacggcgcgc tcaacgtcgg ctgcacccgc
     1681 accgagatcg tcgaggtgct gatccacgcc agcgtctacg ccggattccc ggccgcgctg
     1741 aacggactca cggctgcgaa agaggtcttc gccgcccgta ccgatctgcc cggcgagccc
     1801 gcgccggaac cggctccggc gggtgcgcgg ttcgagcggg gcacggcggc gctgtccgag
     1861 atcgacggcg aagccggcga gcaggtcgtc gccgcattgc aggacatcgc cccggatttc
     1921 gcgcggctgc tcgtcgagtt ctccttcggc gatatctatg cccggcccgg gctcgacctg
     1981 aagacccgcg aaatggtgac ggtggcgatg tgcaccgccc tcggcgtcgc accccaactc
     2041 ggcgtgcata tccacggcct gctcaacgtc ggcggcagcg agcaggaagt ggtcgaggtc
     2101 ttgatccaga tggcgggcta cgccggcttc ccggccgcgc tgaacggaat cgcggtggcg
     2161 cgcacggtct tcgccgagcg ccgcagccat cgaaatgccg atgcctgata tcggcgatcc
     2221 cattggccac cttccgtgcc cgtacctagg gtcggagaca tggcgaaaat actgttcgta
     2281 ctgaccggtg ccgaccactg gaccctggcc gacggcacgc tgcatcccac cggcttctgg
     2341 gccgaggagt tcgcggtgcc ctacgaggcg gccacagcgg ctggccacga gatcgtggtc
     2401 gccacgcccg gcggtctcgt gccaccggtg gacaagggca gcctggccgc cgagtacaac
     2461 ggcggtcagg acatcgccga caaacgggcc gaaacactcg cgaacgccgt cgaattgcag
     2521 cggcccgccg atctcgccca ggtcgacctc gccgactacg acgcggtcta ctacccgggc
     2581 gggcacgggc cgatggaaga cctcgcggtg aaccgcgaat ccggcgaact cctcaccgcc
     2641 gcactgcgat ccggcaaacc gctcgcggtc gtctgccacg gtccggccgc cctgctcgcc
     2701 gccacgaacg ccgacggaac caacgccttc accggctacg ccctcaccgg tttcaccaat
     2761 gccgaggaac ggcagggcgg cctcgcggac aaggccaagt ggctgcttca ggaccggctc
     2821 gtcgaactag gcgcggattt ccaggagggc gagccgtggg cgccgaaggt ggtggtggac
     2881 cgcaacctga tcaccggcca gaatccggcg tcgtccgcac cggtcgccgc ggagctgctg
     2941 aagaagcttg cctgagcggc cacgggaatc accggcgggg tcgagacgtt ggtcgcgata
     3001 gtgacgtcgt cacgtctgac ccgcagtaag gctcgtccgc tttcgcgtaa ttcccctcgg
     3061 aaggaaccgc atcatatgag caccgacctc aacgcccggc ccgccgccgc gtccgggacc
     3121 ttcgcgatcg gcgcagacct gcccgtccat cggctcggtt tcggcgccat gcaactgacc
     3181 ggtccgggcg tctggggtga tccggccgat cccgacgagg cggtgcgagt gctgcgccgg
     3241 gtcgtcgaac tcggcgtgac cttcatcgac accgccgact cctacggccc gttcgtcagc
     3301 gagcagctca tccggaaggc cttgcacccg taccccgacg acctcgtgat cgccaccaag
     3361 ggcggcttca cccgcagcgg ccccggcgat tggcgggcgg tcggccggcc cgaatacctg
     3421 cgtcagcaaa ccgaactcag cctgcgccac ctcgggctgg accggatcga cctctaccag
     3481 ctgcatcgga tcgatccgca ggtgccgctg gccgaccagc tcggcgagct ggcgctgctc
     3541 cagcaggagg gcaagatccg gcacatcggc ctgtcccagg tgagcgtcgc gcagctggcg
     3601 caggcgcgcg agatcgccac catcgtgtcg gtgcagaacc tctacaacct ggccaaccgc
     3661 gacgacgagg acgtgctgga ctacgccgaa cgcgagaaca tcgcgttcat cccgtggtac
     3721 ccgatcgcca ccggcaagtt ggccgcgccc ggcggtccgc tcgatgccat cgccgcggag
     3781 cacggtgctt cgcccgcaca gctggcactg gcctggctgc tgcggcgctc gccggtgctg
     3841 ttgccgatcc cggggacgtc cagtgtcgcg cacgccgagg agaacaccga ggccgcgcgg
     3901 atcacactga ccgacaaaga attcgaaagt ctcgccgcgg cggtgtgagt caccgtcgcg
     3961 cgcgttcccc gaaactggac agtctcgacg gagactgtcc agttttgttt ttcgcggctc
     4021 gatgattgcc gggaccgctt tgcgcgcacc acaattcccc gcgataccgg gggaaatgcg
     4081 gcattgttca cctccatgca atgtcggtgc acccggcacc gaaggcgcca accggaatca
     4141 cgctcgacac ggatggatca ggccgtgccg ggaccgcgcg ctggattccg gaatgccggc
     4201 gccctcgtga gggtttgggc agaaccccgg cgccgggtgc gtgattcagc gtgcacgttg
     4261 cgattcggtg gataacagtc ccgaatgcgg taattgcggg tagtttccgt ttgtatcagc
     4321 ctggtgcgag gaagcgggac ctatggttga gatcgactgg acgggcgagg atgtccgtgc
     4381 gcttcgcgcc gcgatgcggc gaagccggta cgaattcgcg cgtctggtgg gcgtcacccc
     4441 gcgcaccgtt gtcctgtggg aaaacggccg gacgaatcgg atgcatgccg ccagccagcg
     4501 tctgctggac aaggtcgtgt ccgatgccga tccggccgtc gtcgcgcgct tcgagcggac
     4561 tgtcacggca ggtcggggct ccggcttcga cctcgcggac tccgacccgg cgcccgatcc
     4621 gcacacccag ctcggtaggg acatcgggct cgacaaggaa gtcgaggatg acgacgtgag
     4681 aaggcgagag ttattggcct gcatcggcgc gggcctgacc ggccgggccg ccgagctgat
     4741 cgcgagcgag ccggagaaga tgctggccac cctggatgcc gggtcgatga gcgagcggcg
     4801 gctcggcttg ctcgagcact cggccgaaga actcggcaaa cgggtggtgc atctaccgcc
     4861 gcacgaactg ctgcacccca cgctggagca gttccgcacg gtccgtggcg cgctcgccca
     4921 gcggcaggcg acccatcacc aggtccgcct ggtccgcacg gcggggcgac tggccaacgt
     4981 ggtcggcgaa atcctgttca acgaaggaca tttcggacaa gcccggatgt ggtacggcac
     5041 ggcgatccat gccgcgcagg acatcggcga ccggtactcg gaggatatcg cgctggccgg
     5101 tctcgcgtat ctgccgacct acagcgacaa accgaaggaa gtgctgaacc tgctcggcgg
     5161 gcggctcgac gacaatccgg cacacggacc cgcggcggcc tggctgtggg gaatggcggc
     5221 ccgcgcgcac gccagcctcg gaaatgccga ccagttcacg cggtgcatcg agaaatccgc
     5281 gcgcgcattg gaagatattg accagcaaga acttacggtt ggcatttttt cttatcgccc
     5341 ggagaaactc gccttctatc aggcgatcgg taacgcgcga ctcggccgcg ccggcgaaac
     5401 cgcgaaagcc gcggcccgcg ccctcaccct gtacgacccg cgagaaacaa tggagcccgc
     5461 gctcgttcgc ttcgagcacg ccaccgcgtt gctgaccgcg ggtgagatcg acgaagcgtg
     5521 ttccattgcg acacaagcga tcaccgggaa caacacctat atcggactga ctgtcaccaa
     5581 gcgcgccaag cagttcgaca ccgcgctgtc cggctttcgc gggcgcgcgg tcaccggctg
     5641 gcgccagcac gcccggtcgg tgctcgaggc gtcccggacc agggcctgac gaaccgtcca
     5701 gggccgttat gaattcgttg catcctgcgg gcgggcgcgg tgcatgacac cggtgagcca
     5761 accgctgatc tgttgtgtct gcgcgggtgt cgggcggccg gactcccagc gacgggcggc
     5821 tgcgatcaac gtctccggca gcgtgggggt gatccgttga ttcgtcatac gtacatgatg
     5881 cactggggcg cagcagagta cgagtagggg gcgaggtgcg tcgaccggca ggggggatgg
     5941 gagccgacgc accccgccta tatgggtggt ttttcgggtt cggcgctacc tcaggaggtt
     6001 tcgttcgacc cggcttcgat gagtgacttg ggacgcatat cggtccaatg ggtttcgacg
     6061 tagtgcaggc acgctttgcg ggccgcggcc ccgtaggcga tcgtccagcc gcccggtacg
     6121 gcggcgaaaa taggccataa cgagtgctcg ccgtcggcat tgatcaagac gtagaactgg
     6181 gcgtcgtcgt tgtcgaaggg gttgttcatc cggtttccta gcttcctggt cgggtcaggg
     6241 ttttcggggg tcgggtgcca cggcgcgctc ccgcagggtg ctgcgggcgc gatcgcgcca
     6301 aaacgtgaaa ggagcgccac gatccgcggg ggctccgcgt cgcgcgcggg tgtgcgcggc
     6361 agacgagcgt cgcccggacg ggcggaactg tggcgagtgc gacgaagtat gcgacggcca
     6421 tcgcggccgg aatccccgcc ggtgccgaac cttccctgag tccggcaccg gcgaagcctg
     6481 acgcagctgc ccgccttcgg cgtcgtggaa ccgtcgatcc ggttcggccg ctgcttggtt
     6541 cagcctactg ggggcgaccg acatttccgg cggttcggga cacaagccgg tgcggatcga
     6601 gccgggtgac gaagtcggga gcggcggccg cgccgaacgg cgctgggccg gaccggttgt
     6661 ggtgagctgt acccccgacg gatacagctc accgggcatg ccgcagtgcc actccgtatg
     6721 tagcaccaga gcctcctcga gcggcaaaac aacagctggc cgatgcccct gcgggcaggc
     6781 atggcgaaca ttaggcgctt gccccaataa atgggggcgt tttcctttaa acaccgggac
     6841 ggtcgaccag ttcgctacgt cgcgggccgg atctcgtctc agggggtgag atgagtcgcg
     6901 gcgtgttcgg cgatcatcac caccgtcgcc tgcggcccac gactcagcgg caccgggacg
     6961 acggacaggt cgacgatcga aaggccggtg acgccgtgca cccggcagcg ttcgtccacg
     7021 accgctcgct catcgtccgg tgggcccatg cggcaggtgc ccgacaggtg ttgcgaggtc
     7081 gccaggttcg cgcgcagcca ggcatcggcc tgtgcgggcg ttcgtggcat cggatccgta
     7141 cgcggttgcg ccgacatgcc gtgcagcagg tccatcgcga ggtcgaccgc ggtgcgcagc
     7201 cgggcgcgat cgcgctcggc gcgtagataa ccgagccgga tatccggcgg ggtcgccggg
     7261 tcgggcgcac gcagccgcac cgtgcccgcg gactccggtc gcatcagcgc caccccgagc
     7321 cgataggtgc ccggcgtgaa agcgaccgta taaggccgga attcgaggtc gtccactgcc
     7381 agtgcgtatt ccaacgcgac ggccgcccgc gccggtgcgg gcatgcgata ttcgagaccg
     7441 atttctggat ggtcggtgca ccaggcgccg accggggccg gatgcaccac cggaattccc
     7501 gacgctcgga gctgttcggc atcgccgaca cccgaacgca gcagcagcgc cgccgtttcg
     7561 atcgcacccg cactgagcac gatccgatcg gcccacaccg tttcggtgcg accggcgcgc
     7621 agcacgtcga cgccgatcgc acgggtgccc cggaaaacaa tgcgaagcgc cgaagtatca
     7681 ccgtgcacgg tgagattcgg ccgattcagc gcgggcaaca ggtaagcgag cgcggtactg
     7741 gtgcgcaccc cgtcggcgat attgcacggc acccggccca caccgtcggt ggcgccgggc
     7801 gcgttcaaat cggaaagcag cggaaatccc gcgaccacac aggcgttggc gaattcctcg
     7861 ctcagcggta tcggatcggc ggtgcgccgc accggaatcg ggccggagcg gccgtgccag
     7921 cgccggtcac cgaaatccag atcgtgttcc gagcggcgat acgcggacag caccgcatca
     7981 aaggaccaag tgttcgcgcc attcagtacc tggctccacg ccgcgaaatc cgcgggcccg
     8041 gcgcgcacga aatagctgcc gttcaccgcg ctcgaaccgc cgatcgccct gccgcgcacg
     8101 atgtttccgg tctgctcggc actgtccgcc gcagccagcg caatcggata gcgccacaac
     8161 cacggggcgt ccgcctcgac cggcagccgc gtggcatcgc gcagtcgcgc cggaacctcg
     8221 gcgggtgcgc gccacaccgg gcctgcctcg agcagccgca cggtgtggtc cgggtgttca
     8281 ctcagccgcg cggcgagcac acagcccgcc gtaccgccgc cgacgatcag cgtgtcggtc
     8341 atccgccgtc cgatcgagcc gtgggcgaat cagtgcgacg gcgcagtggg tttcagcgat
     8401 tccgggctgc gggcacggaa ggcaccgacc cagagcccgg tgccgtaggc gatatcgtcg
     8461 agccgcttgt aggccacata gcgcaccgga tcgaggccgc ccgcgtcgcg atgggtgaac
     8521 cagtcggcga ggccgtccgc gaccgccatg gtgatcgcga tgcgccgaat tcgcctggag
     8581 gccagcatcg cgagcagcgt caccggccag tagtgccggc acatcgccga ggccagccgc
     8641 cacagcccgg cgaagaagcc gcgcgagagg tagattgcgg cgatccgcgt cgggttgtcc
     8701 agctcggcga acacccggcg tagtcgcacc agcgcggtcg ccagcgtgac cagcccgccg
     8761 agcaggcccc acttggactt ggtggcgaag aggaaggccg ccagcatggt ccacgacggc
     8821 agcgacagcg gcgagaccat ccccgggtgc cgctcgccga gcggcgcggc accggtgccg
     8881 tagaacatct ttcggccgaa ccaggcgcgg aaggacacgc ggtggtcgtg cgcgacatgg
     8941 gccgcgggct cataacgcaa ccgccagccc gcctgctcca gccgccagca cagatcgaca
     9001 tcctcggcga cctgcatcga ctcgtcgaag ccgtcctgcg cgagcaaggc gcggcgacgc
     9061 accagcagcg cggcgctcgg cacgtaggac accagcccgt gcgactgcac ggccgcctca
     9121 cgcctgccca gatccaacga ggagcgggtg tgctcgtagc gggccagtgc gttggactcc
     9181 gggtccagcg cgacgatccg cggggccacc agcgccacct ccggatcgct gaagtgcccg
     9241 agcatcacct cgagccagcc gctgcgcggc accacgtcgg aatccaggaa ggcgacgaag
     9301 tcggttgtcg cggccttcaa tccggcattg cgcgcggccg ccggaccctg ccgctgatcg
     9361 tggcgcagca cggtgacccg gcagcgggtg ccccgatcgc gcgggatgtg caccggctgg
     9421 tccgagccgt cgtcgaccac gatgacgttg tggccgcgca gcgccgcgag cagccgggcc
     9481 agcccgtccg ggttgttgta cagcggcaca atgacggtga cgtcctcggg cgagggcagc
     9541 aggcgcggac gcgggttcgc gacaccggag tcgagcagcc gtctggcaac ggtcgcggac
     9601 ttcggcccgg tcacctcgag gtacccgtcg ccgatcatct cggcggcctc cggggcaagt
     9661 cgcagcaacc gggcggggga accgccgatc agcactcgtc ctccggagta tgcgcgtacc
     9721 cgtggatcaa tccgcacccc gaatccgtcg ggcaggcgat catggcgcat tcctggcatg
     9781 ctaggcggag attcgcgaac aagcatcatc agtccggtta aaaggacaga attccgcacg
     9841 aatcgggacc gccggcgcgc cgcgacggat atcgccgaca aagttggcgc ggcgcatttc
     9901 acacgattcc agcattcggc aacgcgacgg caatcggccg gtcgaaaccg gaactcgact
     9961 gccgcgcgtt cgtcagcaca cgactggcaa ttgccatcgc ttaagccagc ggactctcgt
    10021 cacaggcgcg cgccgggcga cgttcaggtg aaaatccgct cgggcgcgtc accgacaggg
    10081 tcaacggcac gggcgcgggt ttgcgtgcgc cggtgcgcgg tgtcgcccgc cgcgaatgat
    10141 cgaccgagga gcgcgggatc agccgcgccg gatcggccag cgccgcctca ccgtacccct
    10201 ggacgcattc cgggtccggt ccgtcggcgg gcaagccggt gaagaacttg gccgccatgc
    10261 agccgccgcg gcacgagtcg tagtggctgc acgacgcgca ggcgccgcca ccgctgggcg
    10321 cacgcagctc cgcgaacagc tcggactcct gccacacggt gcgaaaaccg ccgtccgcca
    10381 ccacgtttcc agcgtggaac cggtcatgga tggcgaacgg gcaggcgtac acgtcgccga
    10441 gcgggtcgac caggcagacc acccgtcccg ccccgcacag gttcagcccg ggcagcgcct
    10501 gaccgaacgc ggacaggtgg aagaacgagt cgccggtgag cacgccctcg ccgttgcgca
    10561 gcagccagtc gtagagcacc cgctgctgct cctgggtcgg atgcaggtcg tcccagacgt
    10621 ccgcgccgcg gccggacggc cgcagcctgg tcaatcgcaa agtcgcgccg tatttttcgg
    10681 cgatcgcccg gaactcgtcg agctgaccga tgttgtgcct ggtcgccacg acggacagtt
    10741 tcgcgtcgcg gaaacccgcc gcggcgaggt tctccagcgc gcggatggcc atcgcgtacg
    10801 aacccggccc gcgcaccgcg tcgttcactg ccgcgtccgc gccgtccagc gaaatctgta
    10861 cgtcgacata gtcactcgcg gccagccggg cggccacctc gggcgtgatc cgaactccgt
    10921 tggtggagaa cttcacaccg acgtggtgcg cggtggcgta gtcgaccagc tcccagaagt
    10981 ccgggcgcac cgtgggttcg ccgccgccga tgttcacata gaacacctgc atgcgctcga
    11041 actcgtcgat cagcgccttg cactgttcgg tgctgagttc gcgcggatcc cgccggccgg
    11101 acgaggacag gcagtgcaca caggacaggt tgcaggcgta ggtcagttcc caggtgaggc
    11161 aaatcggcgc cgccagaccg gattcgaacc gatcgaccag cggtaccgcc gcgtcggacg
    11221 gcgggaccac ccccgccgtc gacggtgccg cgctcacagc agcggcggcg gcgggcgccc
    11281 gcgtagcagc ggcgggcgcc tcgatgatca tctccgaatc gaccagctgt gtcagcgctt
    11341 tcacatactg cgcggaattc tccgccgaga tgcccgcggc ccccagggcc gcccgcgcgg
    11401 actggtgtgc gggcagtgac cgcacgatct cgacgatccg cggactcttc aggaaggaga
    11461 gtttgcgggt gccgaaatgg tagagcaaag caccgaacgg ttccgggcgc agcgccaccc
    11521 gggggtgtag ctgccatcgg tcgtcgagat cgaacatggt cgttccgcct gtcagtagac
    11581 gccgcacata ccgtcgatgg aaacctcatc gatgaggctc tcctcgacca gcggcgcggt
    11641 gtcctcgcgc ggttcggtgg tgccgaccgt gcccgagtgg tcggtctcgg cgtgtccgtt
    11701 ggctccgtat tccggcatgt ctacctccga gcaagcgtcc ggtggctacc cgatgtagcc
    11761 ccggtcacac gataacgtca ttcgctgaca aaatggaaca cgttcttgtg tgtgacttcg
    11821 ccgccgcagg cagaacggcc tgcccgcacc cacatcgatc ggccggagtc ggtcgtcacc
    11881 attgcgacgg agtcacccgc acagcgaccc gggcgcggcc gcgccctcta gacttcggag
    11941 agaaagcagc tgaacaggac gggacgggcg acaggcgccc gcggtggtag aggtggcatg
    12001 ggagtcggac gaatgcaggc gagcgggggc ggcaacactc tgtcggagtc cgagatcgtc
    12061 gaggccgcgc tgcgagtggt ccgcgaagac ggcgtggaga aactgtcgat gcgacggctc
    12121 tcccgcgaac tcggagtttc cccgatggcg ccgtattact acgtcgcgga caaacgcgag
    12181 ctgctcgacc tggtcgccac cgccgcgctc accggagtgc gcacgccacc agccgaatcc
    12241 ggcccgtggc agatccggct gcgcgatctc atcgaccaga tcgacgagaa gctgcgcaag
    12301 caccccggcc tcggcgacgt actgatcgag cagatgctcg gcaaacaact cgacctgatc
    12361 gcggccatca tggagatcct gttcgaggcg ggcttcaacg accgcaatgt gctcgccgcc
    12421 tatgccacca tccacaccta cctgttcgga cgcagccggg tgaacccgcg cgaccgcgcg
    12481 ccgctgtcgg atgtcgcact cccgaatgcc gtcgaacgtg ccaccaagca catggccgac
    12541 ctgcgcggga agtacgccta cgacttcggc atggaggtgc tgatcgccgg actggaggcc
    12601 cagcttgtcc ggcagccgac gtccgacgta cgataaaact gaaacacgtt ctagttttgg
    12661 cgccgagaag cgcctgtgcc aggttcgaga ggacgaatat ggatgcagca tcgcggagcg
    12721 atcctgtggg gggcggcggt cgggcgacgg gtgggccaac cgtcgaggtt ccacacggct
    12781 cacgttcggt cgtgctgcgg gtagcagcgg tcatcacgga aacgcccgat acctgttcgc
    12841 tggtcttcga cgtgcccgat gaactcgccg agcggttccg ctaccacccc ggccagttcc
    12901 tgaccctgcg cattcccagc gatctgacgg gctcggtggc gcggtgctat tcactggcca
    12961 gctcgccgtt caccgacgac cacccgaagg tcaccgtcaa gcggaccgcc ggcggctacg
    13021 gctcgaactg ggtgtgcgac aacgtgaaag cgggcgactc gctcgaactg ctgccgccct
    13081 caggtgtttt cacgccgaag gatctcgacg cggacctgct gctgttcggc gcgggcagcg
    13141 gcatcacgcc ggtgatgtcg atcctgaaat cggcgctcgc gcggggcagc ggccgaatcg
    13201 tgctggtgta cgccaaccgt gaccacgaat cggtgatctt cgccgacgaa ctgcgtgacc
    13261 tggccgacaa acatccgcag cgcctgaccg tgatccactg gctcgagcac ctgcagggcc
    13321 tgccgaccgc cgacgcgctg gccaccctgg cctcgcccta ccagagcttc gaagcgttca
    13381 tgtgcgggcc gaaaccgttc atggaccggg tgcacgacgc gctcgcccag ctcggcgtgc
    13441 cgcgctcgcg cacgcacgcc gaggtgttca attcccttgc gggcgacccg ttcgcggacc
    13501 aggcgccggt cgaggtgtcc gacgcggaag cggccgaggc cgccacggtc gaggtggaac
    13561 tcgacggcga ggtgcacgag ctgtcctggc cgcgcaagca gaccctggtg gacatcatgc
    13621 tggccaaggg tctggacgtg ccgtactcct gccaggaggg cgaatgcggg tcgtgcgcgt
    13681 gcacggtgct cgagggcaag gtcgagatgg acaacgccga gatcctcgac cccgaggaca
    13741 tcgagaacgg ttatatcctc ggctgccagg cacatccggt gaccgatcac ctcaagatcc
    13801 agttctgatc gatcagcgcg cgcgatagtg tgcgcgcacc gcgggatcga gatgttcggt
    13861 cgcatagctg agcgcggtgc gaccgagatc ggcggcatgc tggtccagga agccggtgag
    13921 caggcgctga tcgatgcgct tgcccacctc gcgcagcatc cagccgaggg ccttctgaat
    13981 gagatcgcgg cgatcgtcga gcagccgctc ggacagcgcg aatgtggttg tggcgtcccc
    14041 ggatttgatg aaggcgaagg tcgagagcag cgcgacccgc cgctcccaca gcgagtccgc
    14101 ggcggccagc tcgaacagca gctcgcgtgg cttgtccagc agccacgggc cgatgatgtt
    14161 ctcggcggac acgtcgacca gatcccagtt gttcacccgg ccacggcgca cggcggccag
    14221 atagcggtcc accatttccg cgcgggccgc gtcgtcgaag gtgcgcggct tggacgcctt
    14281 ggcgaaccgg gcattgagca cgaccaaccc ggcgaggcgg tcctcgtgca cggagctgtc
    14341 cagcagtagg tcgatctcgt cgaggggcag tgccgcaaag cgtttcgcga caccgcgggt
    14401 cgccgggacg cgcacgccga cgaacacgtc gccctcgccg tactcaccgg ggccggtctt
    14461 gaagaaccgt tgcagatgga tcgcgtcggc gggatcggcg atcgcccgca gcgcggcctg
    14521 caccgcggcc gcggtctgcg cgccttcggt catcgcggac acctcccggt gggtcaactc
    14581 agccctgggc gcccaggatg cggccggatt cgaaagccgc ggcgctggcg ggcaactcgc
    14641 cgggcctgcc gctgttgcgg accagcaccg cgccgttgcc cggcgtcggg cggccgagtg
    14701 cgtccatgcg ccggatggtc tgcgcggcaa cggcttgcgc gctgtcgaag agccggacgc
    14761 cgtcgggcag cgcggcgacg atggcatcga tcaccagcgg atagtgcgta cagccgagca
    14821 cgacgccttc gacgtcgtcg ggggtctccg cgaccgcccg tgcgatggac tcgcgcgcgc
    14881 cgaccaggtc accgcggtcg atcgcatcgg ccagaccgtg acaggccacc ccgaccacgt
    14941 cggcgttgcc gccgaacttc gcgatcagat ccgcctggta ccggctggcg gtggtggccg
    15001 cggtggccca caccgccacc gaccggcaga cggccgcggc cggtttgatc gcgggcacgg
    15061 tgccgatcac cggcacatcg gggccgacct ccgcgcgcac atgctccagg gcggtgacgc
    15121 tggccgtgtt gcacgggatg acgatgactt cggcgcccag ctcgatcgac atccccgcgg
    15181 cccggaccac gcggtcgacc gtccactgct ccggtttcgg cccccacggc gcaccgtccg
    15241 ggtccaattg cagcagcaga tccacgtccg gccgcagctt gcgcagccaa gcggatgtgg
    15301 gcagcaagcc gagacccgaa tcgatgagcg cgacgatcac atcgacaacc gtattacagg
    15361 gcccggacgc ggtgcccacg acggcggccg gagtggcagg cgggcgtcgg ccccggcctc
    15421 cggcgcgggc actcggctgc accgcgcgcc cgtacccgga cggcgctcag ctcggcgtga
    15481 gcaactccga aaccggtgcg cccgcggcga tcttcgccct ggtcttcatg acctgtgcgg
    15541 tcgtgcccgc gccgaccacg ccggtcagcc tgccgtcccg ggagtagtag gcgaggaact
    15601 tgcggccgtc gtcgacggcc acatcgacat cgtcggtcgc ggccggcgtg ccgagcgcct
    15661 ggatcttcat gtcgtactgg tcgctccaga agtacggcac ccgggccgcg gtcggcgcgg
    15721 cggcgccgag caacgcgcag gcgaccagcc gcgcctgctc gcccgcgttg gtccagtgtt
    15781 cgatccgctt gcggtgcccg gcctcctgtt gccaggcggc gacatcgccg accgcccaga
    15841 cccgctcggc cgaggtgcgc cccacctcgt cggccagcac cccgccgccc gccgccggat
    15901 cggcgagcgc gatgccggat tcggccagcc actccgtcac cggacgcgag ccgaccccga
    15961 tcaccaccag atcggcggcc acctccgtgc cgtcggacaa gatcgcgccg cgcacccggt
    16021 cgcctgccgc gagcagcgag tgcagcccca ctccgcagcg caggtcgacg ccttcggcgc
    16081 ggtgcatcct ggccaccaac tcgccgaccc gttcgccgag cacaccggcc agcggcgccg
    16141 gctgcggctc gaccagcacc accgcgacgc cgcgggcccg gaaactggcc gcgagctcgc
    16201 agccgatgaa acctgcgccg atcacgagtg ccgttgtcgc cgtggccaat tcgctacgga
    16261 gggcgagggc gtcggcgtgg ccgcgcagca cgtgcacgcc cgccaggtcg ggcagaccgg
    16321 gcaggcggcg cgggcgcagg ccggtggcga tgatcagctg tgcgtagtcg agggcagtgc
    16381 cgtcggcgag gcgtacctgc cgcgccgcgg tatccaggcc gaccgcctcg acgccgagcc
    16441 gcacatcgat ctgcttctcg gtgtagaact cgtcggggcg cagtgtggtg tcgtcggttt
    16501 cgccgcggac gaactgcttg gacagcggcg gccggtcgta gggcaggcgc ggttcgtcgc
    16561 ccagcaggac gagcgcaccc tcgtatccgg tacggcgcaa ttcctcggcg gtgcgcaggc
    16621 cggcgaggcc ggcgcccacg atcacgatcg gcgcggtgtc tgttgctgaa ggtgccacgc
    16681 agctcaccct aacggttgct tggtgccctg ccggagtgag cccgctcact cggcaatcgc
    16741 ggcgtaatcg tagccgttct gatattgatc gggcggtcaa tcaggtctat acccagaggt
    16801 aggacataat cctgagccct aggaggcaca ccatgttcac cgaaagccgc gcacaggact
    16861 tcctggccgt agtgctgggc gtcttcgccg cactctcacc cctgtggctg gaaaccaccg
    16921 acaaagcgat gtggtcgctg atcgtgctcg gcgtactcat cgcactcacc gggctggcgc
    16981 agatgtaccg gcccgcgctc ggcgcgaacg ccgactacgc gatgggcctg ttcggcgtgc
    17041 tgctgttcat ttccccgtgg gccatggact tcagcgatta ccgcggcgca tcctggaccg
    17101 catgggtagt cggggtagtg acggcggtgg tcgcggtagc ggcgctgccg acggtgtcgg
    17161 gacgtctgca caacatggcg ccacaccact gatgcagatg ccgaaacccg gtcggcgggc
    17221 cacccgccga ccgggcaagg aacccgccgc cgggcgtcgc cgcagcgcga aagtggacgg
    17281 cgacgcccgg caactcatcc tggactcggc cgagcagctc ttcgccgaac agggcttcga
    17341 cgccaccgcg acggcggcca tcgccggtgc cgcgggcgtg ccgaaaggcc ttgtcttcta
    17401 ctacttcccg accaaggact cgatcctgtc gaccctgatg gccgagcggg tccccgccca
    17461 cccgatcgaa gatatcggca ccgtggtggc gcccggcgat cccgcggcca gtctggtcgc
    17521 gctggacgcg gcgctgaatc tgcgcgacca ccactcctcg gtgctgcggg tgatcatgtg
    17581 gcgcgaggcc gacacccacc ccgacgtccg gcgccaattg cgccgtctgc gtgatcaatt
    17641 gctcgatgtc accgcacgag tgctccaggc cagcgcacct accccggttc ggcccggcac
    17701 cctgcgcgcc tgcgccgccg cgtgggtctc ggccatgttc gccatcgcca gcaccgaccg
    17761 gctgcatgcc ctcgacggtg tgcccctccc caccgccgac gagttgctca acgtcgccca
    17821 ggtcgtcgcc gccgggatga cccagctcgg ctgaattcgc gaagtgggga tcgaaagtta
    17881 tgcacagggt gcgtgttatc cccaattggg gagcggcggg tggtttccgg gagccgggtg
    17941 gttcacgatc gcggtatgga tgcaccgcaa ctgatctcgc ggcagcaggc gctggcggcg
    18001 gggatctccg atagtgaact gcgcaggcgt tgcctggacg gatcgtggtg gcgggtgcgg
    18061 gccgggcgct acgtcaacgc gcccgagccg gggctcacgc cggccggccg ccatctggtg
    18121 ctcgcactcg cgacctgcga ggcgatgccg gaagacgctg tcgccagcca ctgttcggcg
    18181 gtcgtgctgc acggcgtccc ctgctggtcg atcccgctgg accgcgtgca cctgacccgc
    18241 aaccggatca acggcgggcg ggtcaccaac cagttggtgg tccactcggc acagctgacg
    18301 ccggacgaga tcaccatcgt caacgggctc caggtgacca ccgcaccgcg gacggtcgtc
    18361 gacatcgccc gttcggcgag tttcgagcag gccgtcgtca tcggggacaa cgcgctgcac
    18421 caaggactca ccacagcgga tgaactgcag gagtgccttt cccgcgcgcg acatcgccgg
    18481 ggtgctcgac gggccgcaca caccatcgac ttcctcgacg gtcgcagtgg aagtatcggg
    18541 gagtcgcgca gccgcgtggc cttccgggaa ggcggactgc ctgcgccgga gttgcaggcc
    18601 cgggttttca ccgacgacga cgtttgcgtc ggccgggtcg gcttcctgtt ccccgaactg
    18661 ggggtgatcg gggaattcgg cggaaagatg ccgtgccaga gcgcattacg aacgcctgga
    18721 cagggggtga tcgccgaaaa gaaccgcgcc gaccgactcc gggcgctcgg ctggatggtg
    18781 gtgcggtgga cttggcaaga cctcaccgac ccggcgcacc tggcgcgccg catccgggcc
    18841 gcgggccggg cagctgccta cggccgcagg gctggccact ggacgcctac accgcggacc
    18901 tgagcgcggg gtgggcgagg gctcccgcac tcttttcgtg ttcccgcacc ccgacttgcc
    18961 gagggacacg ccgtgatcag gatcggaaag ggtgcgggaa ctggcgcgac ctcgcagacc
    19021 ctggcgcgcc gcgttcgggc agcggagagg gcagctgcct acggccgcag ggctggccac
    19081 tggacgccta cgccgcgtgg cgtggcgtgg cgtggcgtcg cgggagcggg gcagggcggg
    19141 gcgggcgcgg ttcccgcacc ccttccgtgt tcccgcaccc cgacttgccg agggacacac
    19201 cgtgagcagg atcggaaagg gtgcgggaaa tgacgacccc acagacccgg gcgcgcctgg
    19261 cgccgccgca tccggggcgc tgcctacggc cgccgggcag gccactggac gcctacgccg
    19321 cggggcgggg cggggcgccg cggggcgggg cggggcgggg cgccgcgggg cggggcgggg
    19381 gcggttcccg caccccttct gtgttcccgc accccgactt gccgagggac acacggtgat
    19441 caggatcgga aagggtgcgg gaagcagaac cactcaggcg ctgaggaact cgacgatatc
    19501 ggccgcgaag cgggggtgcc gagtgacgcc ggtgtggctg gtgccggggt agatgatcag
    19561 gcgagaatcc gggatgccgt cggcggttcg ccggaagttc tcggtgctgt agccggcgtc
    19621 ccgctcaccg ccgagaacga gggtggggac gaggatttcg ccgaggcggt ccgagagatc
    19681 gaaatcgtcc tcggcacgga ggaaagcgag agtgtctgcc gggttcttgg ggcgcgccag
    19741 cgggtcgatc aaccacatgg ccgcgcccgc gacccgcgcc aggatgggcg aggagacagt
    19801 cgtcgaagcc atgtggtgta gggcgcgccg gcccgcgaaa gccgcctcgc cgtaacggat
    19861 ctggctttcc ttcgcgagcg gatccaaccg gtaacccgaa gacgcgacga tcaggcggcg
    19921 caccgcggcc ggatgatcca ccgccaattg cagcgccacc gagccaccgg acgaaacacc
    19981 gaccacgtcg accggctcac cgaactcggc gcgcaacgcg tccgcatgct cggcggcgat
    20041 atcggccatc gtcgtgcccg ccgccatgcc gggcgcccgg ctgacggcac agacccggaa
    20101 gtgcctggcc agcggtgcca acatcttgat ttccgaatcc cgcatccacc cccgcggatt
    20161 ggcatgatcc ggcgtgaacc accgcaaaaa caccagcgga cgtccggccc ccatcgccag
    20221 ataaggcatg ccgtgccgca acgcgccctc ggtgacatcg agttccgcgg cgagggtgga
    20281 cttgctcatc gtttcctccg gatctactga tcaacgctcc ccgtcgacgc taaccaaaac
    20341 accgacaccc cgcgataacc cccaaggtca tcgatccccc tcttattccg cccctacgga
    20401 atccataccg ccacaactcg cgcgtacagc cacagaaggc cgcctgtccg ggctgtgcaa
    20461 ggtcatccag tgagtccgca aaggttcggg gaggcatagg gatttcctat ggcgcctgct
    20521 gaatattcgt ctttgcctgt cgttctttcg ccgacatacc gtgaaacaac tcgaccaggt
    20581 gcgcaatcgc gattctccga agatccgcgc ccggggcaca tcgcgccctt ttccgtcgag
    20641 ccgtcaaatg cgcaatcgag aggacagctg acatgaccac ggtcgagaac ggttcaggaa
    20701 caaaggaact cgcgggaaag cgggccctgg tgaccggcgg tacgcgcgga atcggcgccg
    20761 ccgttgtgcg ccaactcctg gacgcgggcg ccgaggtgct gacgaccgcc cggtcggaaa
    20821 cgagcacggt accggagggg gccgccttcg tggcggccga cgtgcgaact cgggctggtg
    20881 cggagacgct cgccgcagcc gcacagggga tgttcggcgg cgtggacatc ctggtccaca
    20941 acgcgggcgg ggcgagtcct taccaaggcg cactggcgat cccggaccaa gtgtggcagg
    21001 acgcactgga cttgaacttc ctggcgtcgg tgcggctgga ctcgctggtg gcaccgggga
    21061 tgcgggaccg gcgttcgggg acgatcgtgc acatctcctc ggccgcagtc cccaccgtgg
    21121 cgccgccgtt cctgcactac acgacagcga aggcggcgct ggagaactac agccgcggat
    21181 tggctgcgga attggccccg ttcggaatcc gggtcaacac cgtatctccc ggcaaaaccg
    21241 ccacccctgg cggcgaggcg acgcgcgagc agtgggcaca cctggactcg ggaccgggcc
    21301 agaacaacac caccccaccg ctgggccgcg atggccggcc cgccgacatc gcccacgcag
    21361 tgctcttcct cgtctcgccg cgggcaagct ggctgaccgg aagcagcatc gtggtcgacg
    21421 gcggcgaatt ccccaggggc tgaacaaaag cgacgatcac agcactcgcg tagcacgcga
    21481 ctacttggtg gcgacaaccc gccgtaagcg cagcaccgtc tccgtcgtgg acccgcgcct
    21541 ttcagctcgg aaatcgt
//
