LOCUS       NC_018681              10939 bp    DNA     circular CON 21-MAR-2024
DEFINITION  Nocardia brasiliensis ATCC 700358, complete sequence.
ACCESSION   NC_018681
VERSION     NC_018681.1
KEYWORDS    RefSeq.
SOURCE      Nocardia brasiliensis ATCC 700358
  ORGANISM  Nocardia brasiliensis ATCC 700358
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Complete genome sequence of Nocardia brasiliensis HUJEG-1
  JOURNAL   J Bacteriol 194 (10), 2761-2762 (2012)
   PUBMED   22535940
REFERENCE   2  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
REFERENCE   3  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000250675.2-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 01:53:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,551
            CDSs (total)                      :: 8,489
            Genes (coding)                    :: 8,417
            CDSs (with protein)               :: 8,417
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 13 of 72
            Pseudo Genes (incomplete)         :: 63 of 72
            Pseudo Genes (internal stop)      :: 3 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:09:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5963388
            Orig. end                         :: 5974327
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003876.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10939
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="35"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10939
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10939
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5939](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5939
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    335..350
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     gene            complement(1037..1264)
                     /locus_tag="O3I_RS26275"
                     /old_locus_tag="O3I_026140"
     CDS             complement(1037..1264)
                     /NRPS_PKS="Domain: PP-binding (4-63). E-value: 6.5e-11.
                     Score: 34.4. Matches aSDomain:
                     nrpspksdomains_O3I_RS26275_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /locus_tag="O3I_RS26275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026140"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_337587894.1"
                     /sec_met_domain="PP-binding (E-value: 2.9e-07, bitscore:
                     29.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVETDLGMVVPQDEPTQIAVGDLGFDSVAVAIGLVAIEERLGVRL
                     TERELLSCWTIADMVELIDTALRSAAGSAE"
     aSDomain        complement(1076..1252)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O3I_RS26275_PP-binding.1"
                     /evalue="6.50E-11"
                     /label="O3I_RS26275_PP-binding.1"
                     /locus_tag="O3I_RS26275"
                     /protein_end="63"
                     /protein_start="4"
                     /score="34.4"
                     /tool="antismash"
                     /translation="DLGMVVPQDEPTQIAVGDLGFDSVAVAIGLVAIEERLGVRLTERE
                     LLSCWTIADMVELI"
     gene            1459..2574
                     /locus_tag="O3I_RS26280"
                     /old_locus_tag="O3I_026145"
     CDS             1459..2574
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="O3I_RS26280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026145"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_014986033.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2e-06, bitscore:
                     26.2, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPRILVVGGGIAGLTLAAALRRTGIEPTVVERVTRYGDVGYVIT
                     LWPMGRQVVRHLGLGARFDALTVPINTYATHVDGGGPLRVFDFGGRLGDGVPRALRRAE
                     LIELLGSYGGGTQVRMARTVTALDQNERIVLAHFDDGTQAEFDVVVGADGIDSAVRGLV
                     AGPVHMRRSGLRLWTWWTPGHAVAADESHEFWGVGRYFGYNPTTGSVGCAAALPAVGAP
                     ATAAQLRDRLRGVAGPMEWMIDTLGADDRVGCFDLDDLRLPQWRFGRVVLLGDAGAAFL
                     PTAGVGASMAMESALVLAEELSQVHAAQVPAALRRYVQRRRARVDAVQNRSRWLIPVMQ
                     RRTRFGSTMRNAVLRALPQRMVIAEITRWNP"
     gene            complement(2586..3830)
                     /locus_tag="O3I_RS45665"
                     /old_locus_tag="O3I_026150"
     CDS             complement(2586..3830)
                     /GO_function="GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /locus_tag="O3I_RS45665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026150"
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="WP_014986034.1"
                     /transl_table=11
                     /translation="MTGYRAGRRLTELDCVHLRLDSAGHPMHWLLALRLAPGASIAVDR
                     LRARVAERAGAHALYQLCLPERPRRRPVFVAAADPAVRVRHAAVPDHSGLLRVIEQRMA
                     TALPRSVPPWDITLVDEADRGGQTVLLRVHHCLSDGLAAPGFAALVVDTDTAHDYERFV
                     TAQRFPFKRALTWRTCTEVPRQYRAARPLRRAGRVLPFPPADCARRVAEFSIPIRTLRQ
                     HAADALGSSTEYLLAAAAAAYRQVIPGTDRGGLRVMVPVTLDAAARHTGNATVSAFLAV
                     DPAGPGMADQVRAVRERLAAVDPVDQGRALASAGVDLQYLPWRAQTRLMARMVRDTCRL
                     SVSVTPGFAMRRTVFGHDVDATVPFSPLMADELMVTALILRDRLTVGVVSDPRVLPGAV
                     SDFAESLRALLLSPV"
     gene            complement(3827..4762)
                     /locus_tag="O3I_RS26290"
                     /old_locus_tag="O3I_026155"
     CDS             complement(3827..4762)
                     /EC_number="2.3.1.39"
                     /GO_function="GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_AT (14-292). E-value: 9.4e-49.
                     Score: 158.3. Matches aSDomain:
                     nrpspksdomains_O3I_RS26290_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="O3I_RS26290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026155"
                     /product="ACP S-malonyltransferase"
                     /protein_id="WP_141692007.1"
                     /sec_met_domain="PKS_AT (E-value: 1.3e-46, bitscore: 158.3,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDTRPTGSARCALIFPGQGAQRPAMGDRWRDTAQWRIVDEISAW
                     TGRDVAELILRTGEPGLRRTDSAQLAIFTMSLLALDYLTEHHSIGPIHACAGHSLGEYT
                     ALVAAGALTPETGAKLVAARGAAMRIATLRCPGTMAAILGAGSEVVEGFAAAARADGAR
                     VWVANVNGPRQVVVSGTAEGVDAVAQDARLARLKVVRIPVGGAFHTPLMRPAVPLLRRA
                     LAAAEFERTGVAVVANADAGLYTATRWRALAERQLTAPVRWTAVLATLSRLGCTRLVEV
                     GPSRILAKAIGRGDHAPTVRYFGYPAEFEP"
     aSDomain        complement(3887..4720)
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O3I_RS26290_PKS_AT.1"
                     /evalue="9.40E-49"
                     /label="O3I_RS26290_PKS_AT.1"
                     /locus_tag="O3I_RS26290"
                     /protein_end="292"
                     /protein_start="14"
                     /score="158.3"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="IFPGQGAQRPAMGDRWRDTAQWRIVDEISAWTGRDVAELILRTGE
                     PGLRRTDSAQLAIFTMSLLALDYLTEHHSIGPIHACAGHSLGEYTALVAAGALTPETGA
                     KLVAARGAAMRIATLRCPGTMAAILGAGSEVVEGFAAAARADGARVWVANVNGPRQVVV
                     SGTAEGVDAVAQDARLARLKVVRIPVGGAFHTPLMRPAVPLLRRALAAAEFERTGVAVV
                     ANADAGLYTATRWRALAERQLTAPVRWTAVLATLSRLGCTRLVEVGPSRILAKAIG"
     CDS_motif       complement(4226..4264)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O3I_RS26290_0004"
                     /evalue="2.90E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="O3I_RS26290"
                     /protein_end="179"
                     /protein_start="166"
                     /score="14.0"
                     /tool="antismash"
                     /translation="ANVNGPRQVVVSG"
     CDS_motif       complement(4307..4342)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O3I_RS26290_0003"
                     /evalue="9.90E+01"
                     /label="PKSI-AT-mM_m5"
                     /locus_tag="O3I_RS26290"
                     /protein_end="152"
                     /protein_start="140"
                     /score="-2.6"
                     /tool="antismash"
                     /translation="AILGAGSEVVEG"
     CDS_motif       complement(4379..4483)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O3I_RS26290_0002"
                     /evalue="2.70E-15"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="O3I_RS26290"
                     /protein_end="128"
                     /protein_start="93"
                     /score="48.4"
                     /tool="antismash"
                     /translation="ACAGHSLGEYTALVAAGALTPETGAKLVAARGAAM"
     CDS_motif       complement(4679..4723)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O3I_RS26290_0001"
                     /evalue="3.60E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="O3I_RS26290"
                     /protein_end="28"
                     /protein_start="13"
                     /score="16.3"
                     /tool="antismash"
                     /translation="LIFPGQGAQRPAMGD"
     gene            5001..5939
                     /locus_tag="O3I_RS26295"
                     /old_locus_tag="O3I_026160"
     CDS             5001..5939
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="O3I_RS26295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026160"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_014986036.1"
                     /sec_met_domain="AfsA (E-value: 5.4e-22, bitscore: 76.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRSTVHRSAVNEVFLTDAAVLGKREWAVAAMLPRSHVLFNDGGN
                     PDYLDPIAIMEVTRQVTEYVAHAFVQVPDNQFLVFRDMRIEVFAPEFLRAGPAPARVVT
                     IVNARTSERLAQHPVTIIVDGNECARAGGRWLPLPQHYYDYIREKARKKTLARGPQPAG
                     PVDPAALLAPQRVGREHPKNVLLAHGATTTDGGMSFRLVFDPAHPFFFDHPMDHVPGML
                     LLEAARQAGLATLAEHRGVPPQRCLLVGCDVDFGNFCEPDLVTHCTAAIDETCSGAAGS
                     PRQQRVRVGFQQAGAALGTVTLDLLDETGSG"
     gene            5952..7265
                     /locus_tag="O3I_RS26300"
                     /old_locus_tag="O3I_026165"
     CDS             5952..7265
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /locus_tag="O3I_RS26300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026165"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_051066763.1"
                     /transl_table=11
                     /translation="MTTPERVAVVGSGVSGVSAALALHRAGCAVEIFESDDRVGGRVGA
                     ADLDGRAVLLGGKNIGHRYTRLRAMLELLGGTGYEPFGINTARLVAGKLVPLDSARRTA
                     TMSAVARMCDWRDLGKLDGLARKVRATDSARFLGSELAAKLARRDDRPLSAHFGRDTTA
                     NFLRPITVRMNGAEPEESYLGTFGVNMGMLLDRFDQPTTGLATLLARVETCAEIHYRTR
                     VTRVSRAESRIYVEAIDARGRSSRTRYDAVAVCTPAYAAAPLLATLSETAAGLLRSVRY
                     FPATVAVVGYAHDVFAADVRAIALDDQPCSNVGSYGKETRNLVRYTFSGRHGRLSEPTE
                     ADVLELVTETEQRLRTHLGTSPQPRTAAAIRHWPRAYCAYLPHYGTFLAALAAAVAPLT
                     GVALAGDYLLGTSLEACCRSGEDAAAALLAALPQGAGR"
     gene            7262..8383
                     /locus_tag="O3I_RS26305"
                     /old_locus_tag="O3I_026170"
     CDS             7262..8383
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="O3I_RS26305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026170"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_014986038.1"
                     /transl_table=11
                     /translation="MRILIVGGGIAGLTLAARLRQRGVRPTVIDTISSYSRHGSAITLW
                     PAGSRVLRDLGLWNSFIHTSAPLRRYVVHDRDGAVLRTFDLERLGARAGLPRATPRAEL
                     LAILESANGGTPVDLETEVARMRQIGSTVRVRFADGRERDFDLVVGADGIRSRTRQQAR
                     LHTAERRFGWRIWCWWARRGSTPPGELHETWGLRRIVAAYPTQDRVACFVAISGAFDGT
                     VPQHWPPSDRRRIDDCVILRTLRDADRVECLDLVEVTVPHWVRGRVVLLGDAAAGLVPT
                     TGIGASLAMESAAVLDDELVRVDAAHIDIALTRFARRRMPRVDAEQHRARVVGDVMMLE
                     SPIARHFRDLGLRYFPTPVLCRELDRWNRGRTP"
     misc_feature    7526..7528
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8380..9000
                     /locus_tag="O3I_RS26310"
                     /old_locus_tag="O3I_026175"
     CDS             8380..9000
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS26310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O3I_026175"
                     /product="hypothetical protein"
                     /protein_id="WP_014986039.1"
                     /transl_table=11
                     /translation="MTTPIDDIPGLFRCETVSRADATGELLALTKRLRNGPLDQDVYTV
                     HQHVDCPPEVLYDYLLDIRSVEEFTYSMRDFRPEGGTGRYVGEDATDPGTKIHLRISGN
                     REVGTVDYESAFDQGDDLWMVFCGRVLSARRVQQRSGALVSWTIWRHPYYDRNPYPHLS
                     PRRGRVWPGSMWPLFHACDVLELRNMRQILEYRLPSAVRGAAR"
     gene            8997..9773
                     /locus_tag="O3I_RS26315"
                     /old_locus_tag="O3I_026180"
     CDS             8997..9773
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /locus_tag="O3I_RS26315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_026180"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_014986040.1"
                     /transl_table=11
                     /translation="MTDIYGAAAEFYDIAGRPYWRPRTAALTAAMSPAAHVDSPIVDVG
                     AGTGLVIEILGAAVPGVPILAIEPSPAMRAALASRVLADPALARRVTIAATTLENTSLP
                     ERFGGLVACGVLGYFPPADRRRLWRLLADRLAPGCRAVVDAAPFPADRTVRPIRVGVAR
                     IGRCRHELWLGSRPAGESGRIELTTLCRVTQDGRTVRESASTQQWWAVDMRTIVAEAAA
                     AGLECRRAGWDLATLRALPAPALDGLRSTAADSIER"
     misc_feature    complement(10229..10243)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.21"
                     /tool="antismash"
     misc_feature    10580..10595
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.9"
                     /tool="antismash"
ORIGIN
        1 atgcgcgcgg tgatcgagga cgatctcgga ccgctggcac gggttttcgc cgatttcgac
       61 gaaaccgcga tcgccgcggc ctcgatcggc caggtgtatc gggctcggtt gcacgacgga
      121 cgccaggtcg cggtcaaggt caaatatccc ggggtggacg ccgccgtcga ggcggacatg
      181 cgcaatctgg cgatgttcag caagctgtgg aagtccatgc tgcccagcgc cgccgacgcc
      241 gaggtgctcg acgagatcgc gcgcaatatc ggcagcgaac tcgactacgt gcgtgaggcg
      301 cggacccagc accgagtggc cacgcgctac cgtggacacc cgttcgtcac ggtgcccgac
      361 agcgtcgaag agtgctgtgg tccacaggtt ttggtgaccg agtacctcga agggcagccc
      421 tgccaagaga ttcggcggct gccgacggcg gatcgggacc ggatcggcga gctgatctat
      481 cgcttctacg tcggatccct gttcaccgat ctcgaattct gcggtgaccc gcatcccggc
      541 aacatcctgc tcgcggccga cggcaggctc ggtttcgtgg atttcggcct ctaccaccgc
      601 atggacccgg agcacgtcga ggtggagcgc gcctgtttgc aggcggcggc tgaatgccgc
      661 tccgacgatc tgttcgcgat gtgggtcgcg cgcggcatca tcgatcccga ctccggtgtc
      721 gacgccgagg aggtgctcga ttacgtgtgg gccgccgccg gttggcacct ggtcgacgag
      781 gagatcacca tcacccccga actcgccacg ggggctttga ttctcgccgt ggaccccagg
      841 gcggccgagt tccgcggcat gcgtaagcag cgactgcccg ccgaacacgt cttctcccgg
      901 cgcgccgacc tgttcacctt cgccgcgctg ggacagctgg agaccaccaa caattggcat
      961 cggatcgccc gcgagtggct ctacgacgag ccgccggtca ccgagatcgg gcgagccacc
     1021 cggcagttcc gctgacctac tcggccgatc cggcggccga gcgcagcgcc gtgtcgatga
     1081 gctcgaccat atcggcgatc gtccagcagg agagcaactc acgttcggtc agccggacgc
     1141 cgaggcgttc ttcgatggcg acgagaccga tcgctaccgc gaccgagtcg aagcccagat
     1201 cgccgacggc gatctgcgtg ggctcgtcct gcggcaccac catcccgaga tcggtctcga
     1261 cgatgtcgag cagcacgtgc agggtggcgt cggacaccgc tccaacctat ccagcggccg
     1321 ggcggcagtc gtggatcgaa tgatccggcc gcgctcaacg aacggcccgg tgcggtgcgg
     1381 agatcaactg ccggacgatg atcgagctcc ggtcgggaaa tagactcgaa gcgtgcacaa
     1441 agcacgaatg gaccggccat gacgccgcga atcctggtgg tgggcggcgg tatcgcgggc
     1501 ctgaccctgg ccgcggcgtt gcggcgcacc ggcatcgagc ccacggttgt cgaacgggtc
     1561 acccggtacg gggatgtcgg gtatgtcatc acgctgtggc cgatgggccg ccaggtggtg
     1621 cgacacctcg gtctcggcgc ccgattcgac gcgctgaccg tgccgatcaa cacctacgcc
     1681 acccacgtcg atggcggcgg gccactacgc gtgttcgact tcggcggccg gctcggcgac
     1741 ggtgtgcccc gggcgctgcg gcgtgccgaa ctcatcgaac tgctcgggtc ctacggcggc
     1801 ggcacccagg tgcggatggc gcgcaccgtc accgccctcg accagaacga acgaatcgtg
     1861 ctggcgcact tcgacgatgg cacgcaggcc gaattcgatg tggtggtcgg tgccgacgga
     1921 atcgattcgg cggtacgcgg gctggtcgcg ggacccgtgc acatgcgccg cagcggcctg
     1981 cggctgtgga cctggtggac gccgggtcac gcggtggcgg ccgacgagtc gcacgaattc
     2041 tggggagtcg gacggtattt cggctacaac cccaccaccg gctcggtagg ctgcgctgcc
     2101 gcgctgccgg ccgtgggcgc gcccgccact gcggcccagc tgcgcgatcg actgcgcggg
     2161 gtggccggac cgatggagtg gatgatcgac acgctcggcg ccgacgatcg cgtcggctgt
     2221 ttcgacctgg acgatctccg gttgccgcag tggcggttcg ggcgtgtggt gttgctcggc
     2281 gacgcgggcg cggcgtttct gccgaccgcg ggcgtcggag cgtcgatggc gatggaatcc
     2341 gcgctggtgc tcgccgagga attgtcgcag gtgcatgccg cccaggtccc ggcggcgttg
     2401 cggcggtacg tgcagcgtcg ccgggccaga gtcgacgcgg tgcagaaccg ctcacgctgg
     2461 ttgatcccgg tgatgcagcg ccgcaccagg ttcggcagca cgatgcgcaa tgccgtgctg
     2521 cgcgcgctgc cgcaacggat ggtgatcgcc gagatcaccc gctggaatcc gtagcccgag
     2581 cgtcgctaca ccgggctgag caacagcgca cgcagacttt cggcgaaatc cgagacggca
     2641 ccgggcaata ctcgaggatc ggagacgacg ccgacggtca gccggtcgcg caggatcaac
     2701 gcggtcacca tcagctcgtc ggccatcagc ggcgagaacg gcaccgtcgc atcgacgtca
     2761 tgcccgaata cggtgcgccg catcgcgaat ccgggcgtga cactgacgct gaggcggcag
     2821 gtgtcgcgca ccatccgtgc catcaatcgg gtctgcgccc gccacggcag atattgcaga
     2881 tccaccccgg ccgacgccag cgcgcggccc tggtccaccg ggtcgacggc ggccaaccgc
     2941 tcccgcaccg ccctgacctg atccgccatc ccaggacccg ccggatcgac cgccaaaaac
     3001 gcggagacag tggcatttcc ggtgtgccgc gccgccgcgt cgagggtcac cggcaccatg
     3061 acgcgcagcc cgccgcgatc ggtaccgggg atcacctggc gataggccgc cgcggccgcc
     3121 gccagcaggt attcggtgct cgagccgagc gcgtccgccg cgtgctgccg caatgtccgg
     3181 atcgggatcg agaattcggc gacccgccgg gcgcagtccg cgggcgggaa cggcagcacc
     3241 cggcccgcgc ggcgcagcgg acgcgcggca cgatactgcc gtggcacctc ggtgcaggtg
     3301 cgccaggtga gtgcgcgctt gaagggaaat cgttgcgcgg taacgaatct ctcgtagtcg
     3361 tgcgcggtgt cggtgtcgac gacgagggcc gcgaagcccg gtgcggccag tccatcgctc
     3421 aggcagtggt gtacgcggag caacaccgtc tggccgccac ggtcggcctc gtcgaccagg
     3481 gtgatgtccc acggcggcac gctgcgcggc agcgcggtgg ccatgcgctg ctcgatcact
     3541 cggagcagac cgctgtgatc gggcacggcg gcgtgccgca cccgcacagc cggatccgcg
     3601 gccgcgacga aaaccggtcg gcggcgcggc cgctcgggca ggcacagctg atacagcgcg
     3661 tgtgccccgg cgcgctcggc cacccgcgcc cgcaaccggt cgaccgcgat cgaagcaccc
     3721 ggcgccagcc gcagcgcgag cagccagtgc atcggatgcc cggcgctgtc gagacgtaga
     3781 tggacgcagt ccaattcggt cagtcgccga ccggcgcgat acccggtcac ggctcgaact
     3841 ccgccgggta gccgaagtac cgcaccgtcg gcgcgtggtc gccgcggccg atggctttcg
     3901 ccagtatccg gctcggcccc acctccacca gccgcgtgca gcccaggcgc gacagcgtcg
     3961 cgagcacagc ggtccagcgc accggcgccg tcagctgccg ttcggccagt gcccgccagc
     4021 gcgtggcggt gtacagcccg gcatcggcgt tcgcgacgac cgccacgccg gtccgctcga
     4081 attcggccgc tgccaaggcc cgccgcagca gcggcaccgc gggccgcatg agcggagtgt
     4141 ggaacgcacc accgaccggt atccgcacca ctttcagccg cgccagcctc gcgtcctgag
     4201 ccaccgcgtc gacaccttcg gcggtgccgg acaccacgac ctggcgcggc ccgttcacgt
     4261 tggccaccca gactcgcgcg ccatcggccc gcgccgcggc cgcgaacccc tcgaccactt
     4321 cgctgcccgc gccgaggatc gcggccatcg tgccgggaca ccgcagcgtc gcgatgcgca
     4381 tcgccgcacc ccgcgcggcc acgagtttcg caccggtctc gggtgtcagt gcccccgccg
     4441 ccaccagtgc ggtgtactcc cccaggctgt gtccggcaca cgcgtggatc gggccgatgg
     4501 agtggtgctc ggtcaggtag tccagcgcca gcaacgacat ggtgaagatc gccaattgcg
     4561 ccgaatccgt tctgcgcaaa ccgggttcgc cggtgcgcaa gatcaactcg gcgacatcac
     4621 gccccgtcca ggcggagatc tcatcgacga tccgccactg cgcagtgtcg cgccaccggt
     4681 cacccatggc cggacgttgc gcgccctggc cgggaaaaat cagggcgcat cgcgccgacc
     4741 cggtgggccg tgtatcgagc acgggacgag cgtaacgccc gctgcggcac cggtattcgg
     4801 tcagacgttg agcacggttc accgaacgca cgatgattcg cgccgctcgg cgtcgatacg
     4861 ctcccgatag ccggaacccg cggccgcgat cccggcccgc tactcgacga gaggcaaccg
     4921 tggacacgac cgcaacgacg actcgtactt ccgccgatag cagccgaaac acactgtcgg
     4981 agttacgctt cgagcagacc gtgccgcgtt cgacggtgca ccgcagcgcc gtcaacgagg
     5041 tgttcctcac cgacgccgcg gtgctcggca agcgcgaatg ggcggtggcc gcgatgctgc
     5101 cgcgcagcca cgtgttgttc aacgacggcg gcaatccgga ctacctggat ccgatcgcga
     5161 tcatggaggt cacccgtcag gtgaccgagt acgtggcgca cgcgttcgtc caggttccgg
     5221 acaatcagtt cctggtcttc cgcgatatgc ggatcgaggt cttcgccccc gagttcctgc
     5281 gggccgggcc cgcgcccgcg cgggtggtga ccatcgtcaa cgcccgtacc tccgagcggc
     5341 tcgcgcagca cccggtgacg atcatcgtcg acggcaacga atgcgcccgg gccggcggcc
     5401 ggtggctgcc gctaccccag cactactacg actacatccg ggagaaggcc cgcaagaaga
     5461 cgctcgcacg cgggccccaa cccgcggggc cggtcgaccc cgccgcgctg ctcgcaccgc
     5521 agcgggtcgg ccgggagcac ccgaagaacg tcctgctcgc gcacggcgcg acgaccacag
     5581 acggcggcat gtccttccgg ctggtcttcg atccggcgca cccgttcttc ttcgaccatc
     5641 cgatggacca cgtgcccggt atgttgctgc tggaggccgc ccgccaggcg ggtctggcaa
     5701 ccttggccga acaccggggc gtgccgccgc agcgctgtct gctcgtcggg tgcgacgtcg
     5761 atttcggcaa cttctgcgag cccgacctgg tcacgcactg cacggccgcg atcgacgaaa
     5821 catgttccgg cgctgctggt tccccccggc aacagcgcgt gcgcgtcggc ttccagcagg
     5881 cgggcgcggc gctcggcacc gtcaccctcg acctgctcga cgaaaccggt tcgggctgaa
     5941 ggggtactaa cgtgacgaca ccggaacgag tcgcggtggt ggggtccggt gtctccgggg
     6001 tctccgccgc attggcgctg caccgggccg gctgcgcggt ggagatcttc gaatccgacg
     6061 accgggtcgg cggccgcgtc ggcgcggccg acctcgacgg acgcgcagtg ctgctgggcg
     6121 gcaagaacat cgggcaccgc tacacccggc tgcgggcgat gctcgaactg ctgggcggga
     6181 ccggatacga gccgttcggc atcaacacgg ctcgcctcgt agcgggcaaa ctggtcccgc
     6241 tcgacagcgc gcgccggacc gcgaccatga gtgccgttgc gcgcatgtgt gattggcgcg
     6301 atctgggcaa gctcgacggg ctggccagga aggtccgcgc gacggactcg gcccgcttcc
     6361 tcggctccga gctggccgcg aagctggcac gccgggacga ccggccactg tcggcgcatt
     6421 tcggccgcga taccaccgcc aacttcctcc ggccgatcac ggtgcggatg aacggcgccg
     6481 aacccgagga gagctacctc ggcactttcg gcgtcaacat gggcatgctg ctcgaccgct
     6541 tcgaccagcc gaccaccggc ctggccacac tgctggcacg ggtcgagacg tgcgccgaga
     6601 tccattaccg cactagggtc acccgtgtct cgcgagccga gagccggata tacgtcgagg
     6661 cgatcgatgc ccgcggccgg tccagccgga cgcgatatga cgccgtcgcg gtgtgcacgc
     6721 ccgcctacgc ggcggcgccg ctgctggcca cgctctcgga aacggcggcg ggcctgctgc
     6781 gttcggtccg gtactttccg gccacggtcg cggtggtggg ctacgcgcac gacgtcttcg
     6841 ccgccgatgt gcgcgcgatc gcactggacg accagccgtg cagcaatgtc ggcagctacg
     6901 gcaaagagac gagaaacctt gtccggtaca ccttctccgg taggcacggc cgcctgagcg
     6961 aacccaccga ggccgacgtg ctcgagctgg tcaccgaaac cgaacagcgc ctgcgcaccc
     7021 acctcggcac gtcgccgcag cccagaaccg cagcggcgat ccggcattgg ccgcgcgcgt
     7081 attgcgccta cctgccgcac tacggcacct tcttggccgc cctcgccgcc gcggtcgcgc
     7141 cgctcaccgg cgtcgccctg gccggtgact atctgctcgg cacctcgctc gaagcctgct
     7201 gccgctccgg cgaagacgcg gccgcggcac tgcttgcggc gctgccgcag ggagcgggca
     7261 ggtgagaatc ctgatcgtcg gcggcggcat cgccgggctg accctggccg cgcggctgcg
     7321 ccagcgcggc gtgcggccca ccgtgatcga cacgatcagc agttacagcc gccacggttc
     7381 cgcgatcacc ctgtggccgg cgggcagtcg ggtgctgcgt gacctcggcc tgtggaactc
     7441 gttcatccac accagcgcgc cattgcggcg ctatgtcgtg cacgatcgcg acggcgccgt
     7501 gctgcgcacc ttcgatctcg aacgattagg cgcgcgagcg ggtttgccgc gggccacccc
     7561 gcgcgccgag ttgctcgcca tcctcgagtc cgccaacggc ggcacgccgg tcgatctgga
     7621 gaccgaggtg gccaggatgc gccagatcgg ttcgaccgtg cgggtgcgct tcgccgacgg
     7681 gcgcgagcgc gatttcgacc tcgtcgtcgg cgccgacggc atccgctcgc gcaccaggca
     7741 gcaggcgcgg ttgcataccg ccgaacgccg cttcggctgg cgcatctggt gctggtgggc
     7801 ccgccgcggc agcacgccgc cgggcgagtt gcacgagacc tggggcctgc ggcgcatcgt
     7861 ggccgcgtac ccgacccagg atcgggtggc ctgtttcgtg gcgatctcgg gtgcgttcga
     7921 cggcacggtt ccccagcact ggccaccgag cgaccgccgc cgcatcgacg actgcgtgat
     7981 cctgcggacg ttgcgcgacg ccgaccgggt cgaatgcctc gacctggtgg aagtcaccgt
     8041 cccccactgg gttcggggac gcgtcgtgtt gctcggagac gccgcggccg ggctggtgcc
     8101 gaccaccggc atcggggcgt cattggcgat ggaatcggcg gcggtgctcg acgacgaact
     8161 ggtgcgcgtc gacgccgcgc atatcgatat cgccctcacc agattcgcgc gccgccgcat
     8221 gccccgggta gacgccgaac agcaccgcgc ccgggtggtc ggcgacgtca tgatgctcga
     8281 atcccctatc gcgcggcact tccgggacct cgggttgcgc tatttcccga cgcccgtgct
     8341 gtgccgcgag ctcgaccgct ggaaccgagg gagaacacca tgaccacccc gatcgatgac
     8401 attcccgggc tgttccgatg cgagacggtc tcgcgcgccg acgccaccgg cgaattgctg
     8461 gcgttgacca aacgactgcg caacggtccg ctcgaccagg acgtgtacac cgtgcatcag
     8521 cacgtcgatt gcccgcccga agtgctctac gactatctcc tcgatattcg cagcgtggag
     8581 gaattcacct acagcatgcg cgatttccgc cccgagggcg gcaccgggcg ctatgtcggc
     8641 gaggacgcca ccgatcccgg cacgaagatc cacctgcgca tcagcggtaa ccgcgaggtc
     8701 ggcaccgtcg actacgaatc ggcgttcgat cagggcgacg atctgtggat ggtgttctgc
     8761 ggtcgcgtgt tgtccgcacg ccgggtgcag cagcgcagcg gcgcactggt gtcgtggacg
     8821 atctggcggc atccgtatta cgaccgcaat ccgtatccgc acctgtcccc gcgccgtggc
     8881 cgggtctggc ccgggtcgat gtggcccctg ttccacgcct gcgatgtgct cgaactgcgc
     8941 aatatgcgac agattctcga atatcgcctg ccctccgccg tgcggggtgc ggcgcgatga
     9001 ccgatatcta cggtgccgcg gccgagttct acgatatcgc cggtcgcccg tactggaggc
     9061 cgcgcaccgc ggccctgacg gccgcgatgt cccctgccgc acacgttgat tcgcccattg
     9121 tggacgtcgg cgcgggcacc gggctggtca tcgagatcct cggcgctgcg gtgcccggcg
     9181 tcccgatcct cgcgatcgag ccctcacccg ccatgcgcgc ggcgctcgcg agccgcgtgc
     9241 tcgcggatcc ggcactggcc cggcgggtga ccatcgccgc gaccacactc gaaaacacct
     9301 cgctaccaga gcgattcggc ggattggtcg cctgcggcgt actcggctac tttccgcccg
     9361 cggaccggcg gcggttgtgg cggctgctcg ccgaccgact cgccccgggc tgtcgcgcgg
     9421 tcgtggacgc cgcgccgttt cccgccgatc gcacggtgcg gccgatccgg gtcggtgtcg
     9481 cgcgcatcgg ccggtgccgt cacgaactgt ggctcggatc gcgccccgcg ggtgaatcgg
     9541 gacggatcga actgaccacg ctctgccggg tgacgcagga cggccgcacg gtgcgggagt
     9601 cggcgagcac gcagcagtgg tgggcggtcg acatgcgcac gatcgtcgcc gaggcggccg
     9661 cggccggact cgaatgtcgc cgcgcgggtt gggatctcgc gaccttgcgg gcactgccgg
     9721 caccggccct cgatggtctg cggagcactg cggcggactc catcgagcgt tgagtcaggt
     9781 gtgatcgatc ggccgaggaa acacagccgt acccaaggct atcgtcaatg cactcggacg
     9841 gatcgcaccc agcacacaag gagtgtcatg ccgaatcaga ttgcttcgga accggattcg
     9901 acattctggc acggattcac caatatgcgc ggcttccgca ggcgcggcat cagcatcgcc
     9961 gccgcggacg gcgtgtgggt ggtggacacc gagggcaagc gatatctcaa cgcgttcggc
    10021 ggtctgtgca atctgagtct cggtggcgcg catccggaaa tcatcgacgc catccgcgag
    10081 caggcgggca agctgctcta cttcccgatc gaacgcgcca cccatcaggc ggccgacgaa
    10141 tacgctcgtc gcctggccgc ggtgacgccg gagggactga acaccgtctt ctacgcctcc
    10201 accgggtccg aggccaacga gaccgtgctg aaaatgatgc gccagtatca gcggctccgc
    10261 ttcggcgacg acaccaccaa actcggtgtg atcgccctgg atcgggcata tcacggcgtc
    10321 acctacggcg cgttgagcgc caccggctcc gcgttccgcc gcttccgcaa acaattcgag
    10381 ccgttgactc ccggcttcca tcacgcgccc tcgccctacg cttaccgctg cgaatccggt
    10441 tgcgccggaa cgtgcacgct cgcgtgtgcg gacacgatcg agctgatgat cgagcggctg
    10501 ggcgcacaca ccatcgcggg cgtgctcgtc gagccggcgc tgggcgtcgg cggcgcgatc
    10561 gtgccgaccc gcgaatacca cgaacggctg cgcgagatct gcgaccgcaa cgacctgctg
    10621 ctcgcctacg acgaggtgat caccggcttc ggcaggctcg gtgagtggtt cggcgccgac
    10681 tacttcgggg tgcggccgga cttcatggcg ctgtccaagg gcatcaacag cggctacctg
    10741 ccgttcggcg cggtcgttgt gcgcgacgtt gtctaccgtg aattcctctc cgccgtagac
    10801 ggtttcatcg cgaccgggag caccacgaac ggcaatccgc tgtgctgtgc gtccgcgctg
    10861 gcgacgctgg aggtcatgca gcgcgatggc atcgtcgatc aggtgcgcca gaccggtaag
    10921 tacctgttcg acaagttcg
//
