LOCUS       NC_018681              10399 bp    DNA     circular CON 21-MAR-2024
DEFINITION  Nocardia brasiliensis ATCC 700358, complete sequence.
ACCESSION   NC_018681
VERSION     NC_018681.1
KEYWORDS    RefSeq.
SOURCE      Nocardia brasiliensis ATCC 700358
  ORGANISM  Nocardia brasiliensis ATCC 700358
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Complete genome sequence of Nocardia brasiliensis HUJEG-1
  JOURNAL   J Bacteriol 194 (10), 2761-2762 (2012)
   PUBMED   22535940
REFERENCE   2  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
REFERENCE   3  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000250675.2-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 01:53:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,551
            CDSs (total)                      :: 8,489
            Genes (coding)                    :: 8,417
            CDSs (with protein)               :: 8,417
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 13 of 72
            Pseudo Genes (incomplete)         :: 63 of 72
            Pseudo Genes (internal stop)      :: 3 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:09:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5877680
            Orig. end                         :: 5888079
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003876.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="34"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            229..1395
                     /locus_tag="O3I_RS25855"
                     /old_locus_tag="O3I_025715"
     CDS             229..1395
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881057.1"
                     /locus_tag="O3I_RS25855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025715"
                     /product="lactate 2-monooxygenase"
                     /protein_id="WP_014985949.1"
                     /transl_table=11
                     /translation="MTSNFGDYQFEIYLQGLAGVQPSFPMTHSELEARAQAALPPGIWS
                     YVAGGAGDELTQRTNVTAFEKYGIVPRMLRATKVRDLSIELFGMRLPAPVFMSPIGVVG
                     LCAQDGHGDIATAKAAARTGVPMVASTLTVDPLEEVAGHFGDTPGMFQLYTPTNRDLAE
                     SLIRRAEQAGFKAIVVTLDTWVTGWRPRDLSTSNFPQLRGHCLANYFTDPVFRGLLAKS
                     PEEDPKTAILTWVALFGGAPTWDDLPWLRSLTDLPLILKGICHPEDARRAKDAGIDGIY
                     CSNHGGRQANGGIPAIEHLPEIVEAVDGLPVLFDSGVRSGADIIKALALGATAVGIGRP
                     YSYGLAIGGVDGIVHVLRTLLAEADLIMAVDGYPTIADLADDAIRRLH"
     gene            complement(1448..3436)
                     /locus_tag="O3I_RS25860"
                     /old_locus_tag="O3I_025720"
     CDS             complement(1448..3436)
                     /NRPS_PKS="Domain: AMP-binding (178-568). E-value: 1.6e-68.
                     Score: 223.1. Matches aSDomain:
                     nrpspksdomains_O3I_RS25860_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046696.1"
                     /locus_tag="O3I_RS25860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025720"
                     /product="AMP-binding protein"
                     /protein_id="WP_014985950.1"
                     /sec_met_domain="AMP-binding (E-value: 3e-71, bitscore:
                     239.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPQTYARMFQGIGDCERAETSDTHPVLLIRARHADTELTVPALR
                     LVVGKELESFELQCPGLGSFAAVRLRGEAEAEPTRVTITYFGAGRIHPWIAEQDNADVI
                     AWTTAGLSRIVDAVLGTPTSVLVNGEESPTKQQVGTLKQMVTTGVVRTYRPDRALKQVG
                     GLARWGFTLAGGYAAAAGHSPNRLAVVDGVSARTFGQMHDRTHKLASALAMLGIGARDK
                     VGLLSRNRVTMVECMVATGKLGVDTVLLNTGLSARQIEDVADRHGLSAVFLDDEYEPLT
                     KYVAASVPRFATGHLTRYDRNTVDDLIALDAPTFARPSHPGRLIVLTSGTSGTPKSAQR
                     PQPKGFGTVAALLSRIPMRMDETMLIPAPLFHTWGLAALQISTPIRASVVLPERFDAED
                     CLRLIEEHRVTALIVVPVMVNRILDLPAHVRDRYDTSSLRVVASCGAPLAGPTVVKFLD
                     AFGDVLYNVYGSTEVSWATIADPADLRAAPTTAGRPPLGTKLAVLDKELRPVPRGVTGR
                     IFVLNHMLFDGYTDAKPPTEWGGMLDTGDLGYLDADGLLFVAGRDDEMIISGGENVFPR
                     PVEEALSHLPQVSEVAVVGVPDQEYGQRLAAFVVTREGFGLDRDMVCNYIRHRLSRFSV
                     PRDVTFLDALPRNATGKILKRTLIQPS"
     aSDomain        complement(1733..2902)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O3I_RS25860_AMP-binding.1"
                     /evalue="1.60E-68"
                     /label="O3I_RS25860_AMP-binding.1"
                     /locus_tag="O3I_RS25860"
                     /protein_end="568"
                     /protein_start="178"
                     /score="223.1"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="AAGHSPNRLAVVDGVSARTFGQMHDRTHKLASALAMLGIGARDKV
                     GLLSRNRVTMVECMVATGKLGVDTVLLNTGLSARQIEDVADRHGLSAVFLDDEYEPLTK
                     YVAASVPRFATGHLTRYDRNTVDDLIALDAPTFARPSHPGRLIVLTSGTSGTPKSAQRP
                     QPKGFGTVAALLSRIPMRMDETMLIPAPLFHTWGLAALQISTPIRASVVLPERFDAEDC
                     LRLIEEHRVTALIVVPVMVNRILDLPAHVRDRYDTSSLRVVASCGAPLAGPTVVKFLDA
                     FGDVLYNVYGSTEVSWATIADPADLRAAPTTAGRPPLGTKLAVLDKELRPVPRGVTGRI
                     FVLNHMLFDGYTDAKPPTEWGGMLDTGDLGYLDADGLLFVAGRDDEMIIS"
     gene            complement(4089..4994)
                     /gene="thpD"
                     /locus_tag="O3I_RS25865"
                     /old_locus_tag="O3I_025725"
     CDS             complement(4089..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006897109.1"
                     /locus_tag="O3I_RS25865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025725"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_014985951.1"
                     /sec_met_domain="PhyH (E-value: 2.6e-32, bitscore: 111.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLQHTETRPSTDRIDRYPTRIDAPATHLDRTDPTVWGRVESPAL
                     AAFDTDGFASIDELLTPTEVADFSAEIGRLAGDPALRDDERVIVEKASNRVRSVFEVHR
                     LSAAIADLVRESRVAGLARQVLGSEVYIHQSRVNYMPGFRGTGFYWHSDFETWHAEDGM
                     PAPRAVSLSIALTDNYPINGSLMVMPGSHRTFVPCLGSTPAEHYRESLQEQEIGVPTQE
                     DITTLANQYGISQFTGRAGSALLFDSNLMHGSSNNITPFPRSNIFLVFNSVENTLVEPF
                     GAPAPRPGYIGSRDFTPLSA"
     gene            complement(5001..5399)
                     /locus_tag="O3I_RS25870"
                     /old_locus_tag="O3I_025730"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930019.1"
                     /locus_tag="O3I_RS25870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025730"
                     /product="ectoine synthase"
                     /protein_id="WP_014985952.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-47, bitscore:
                     159.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTTAEITDTERDVAGEGWRSKRIILGGDGVGFSFHETTIDAG
                     STHEFHYRYHVEAVWLIEGEGTLTDLDNGVTYELGPGSMYLLDGNERHQLSARTRMRMM
                     CVFNPPVTGQEVHDSTGAYPLVAATIGG"
     gene            complement(5396..6673)
                     /gene="ectB"
                     /locus_tag="O3I_RS25875"
                     /old_locus_tag="O3I_025735"
     CDS             complement(5396..6673)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (26-361). E-value: 4.2e-75.
                     Score: 244.8. Matches aSDomain:
                     nrpspksdomains_O3I_RS25875_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351409.1"
                     /locus_tag="O3I_RS25875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025735"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_014985953.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.7e-75, bitscore:
                     252.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITADTSIFESLESNVRGYCRAWPAVFSKAKGSWLRDEDGKDYLD
                     FFAGAGALNYGHNNDVLKQPLLDYIASDGITHGLDMSTSAKRSLLETLRDTVFTPRGLD
                     YKVQFPGPTGANAVEAALKLARKVTGRETIVSFTNAFHGMTLGALSVTGNAAKRAGAGV
                     PLVHAAHMPYDGYFDGTTADFQWMEKVLDDTSSGFDRPAAVIVETVQGEGGVNVARAEW
                     LRHLAGLCEARDILLIVDDVQMGCGRTGPFFSFELAGITPDIVTLSKSIGGYGLPMALV
                     LFKSELDQWSPGEHNGTFRGNNPAFVTADVALRHYWSDDTLQNATLAKGERIASTLTEL
                     AGNVPGVSTRGRGLVHGVVFDDPSQASKVCQIAFERGLLVETSGSTDEVVKLLPALTIT
                     DQELDHGLSILTGAVDTVCNGMGAIA"
     aSDomain        complement(5591..6595)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O3I_RS25875_Aminotran_3.1"
                     /evalue="4.20E-75"
                     /label="O3I_RS25875_Aminotran_3.1"
                     /locus_tag="O3I_RS25875"
                     /protein_end="361"
                     /protein_start="26"
                     /score="244.8"
                     /tool="antismash"
                     /translation="FSKAKGSWLRDEDGKDYLDFFAGAGALNYGHNNDVLKQPLLDYIA
                     SDGITHGLDMSTSAKRSLLETLRDTVFTPRGLDYKVQFPGPTGANAVEAALKLARKVTG
                     RETIVSFTNAFHGMTLGALSVTGNAAKRAGAGVPLVHAAHMPYDGYFDGTTADFQWMEK
                     VLDDTSSGFDRPAAVIVETVQGEGGVNVARAEWLRHLAGLCEARDILLIVDDVQMGCGR
                     TGPFFSFELAGITPDIVTLSKSIGGYGLPMALVLFKSELDQWSPGEHNGTFRGNNPAFV
                     TADVALRHYWSDDTLQNATLAKGERIASTLTELAGNVPGVSTRGRGLVHGVVFD"
     misc_feature    complement(6569..6571)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6670..7266)
                     /gene="ectA"
                     /locus_tag="O3I_RS25880"
                     /old_locus_tag="O3I_025740"
     CDS             complement(6670..7266)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209248.1"
                     /locus_tag="O3I_RS25880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025740"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_081594139.1"
                     /transl_table=11
                     /translation="MSVPTLNTAEIERSRVEQAAAALGPDPGRHRTELVLRKPRVEDGA
                     QLWRIAKNSEVLDVNSSYAYLLWCRDFAETTVVAELQGRVVGFVIGYLRPQAPDTVFVW
                     QVAVDRAQRGRGTGAQLLHALLNTVAPQGVTTLETTISPDNAASIALFGSVARQRDARM
                     TKRPLFDKGVFPDSHESEDLYLIAPIARTTQEDHR"
     gene            complement(7351..8430)
                     /locus_tag="O3I_RS25885"
                     /old_locus_tag="O3I_025745"
     CDS             complement(7351..8430)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005572918.1"
                     /locus_tag="O3I_RS25885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025745"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_014985955.1"
                     /transl_table=11
                     /translation="MSSAERWLDPTRWSLRGRVTAVFTTISALMSLVLVVSVFTISRSY
                     LERAQAGTTDLQANLDLLRNVLVACAVAVTAIGAAVGWWASRRVLTPLHQLAATAARIA
                     SGDLDLRLSATHDQDLATTVESFNSMVDSLQRRIEREHRLFGDVSHELRTPLTTLMASV
                     DVLNRHRDDLPERSQRALALVTEEVDHLRRLLDDLLALARVEAGMHRGDQEPLSVRDLL
                     RHTLAERRYPPELLTVHEDVVVLGRKLELERAVVNLLQNADLHGGGVVAVAADRVGDAA
                     VIVVDDAGPGVPTADRVRIFERFATARSGRRSATGTGIGLALVAETVAKHGGQVECVSR
                     PGGGARFVATLPGVGAIEP"
     gene            complement(8417..9130)
                     /locus_tag="O3I_RS25890"
                     /old_locus_tag="O3I_025750"
     CDS             complement(8417..9130)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351413.1"
                     /locus_tag="O3I_RS25890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_025750"
                     /product="response regulator transcription factor"
                     /protein_id="WP_014985956.1"
                     /transl_table=11
                     /translation="MTANLMIVEDDDRVRGALRLAMEDEGYDVAEAEEAEDALTHLRSN
                     GVPDVMIVDLMLGDMDGFDCIREIRREHDVPIIVVSARDDTHDVVAALEAGADDFVAKP
                     FEIKEITARMRAVRRRARLTAEREAPPQEVVLDADPEAPLVLAQDAGVVRRGEEEIRLT
                     LTEFRLLCELAETPGRVLSRTVLLERVWDQGFFGDERIVDVHMRRLRTKIERDASDPRV
                     VVTVRGLGYRLDVQR"
     misc_feature    complement(9301..9318)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    complement(9325..9340)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.9"
                     /tool="antismash"
     misc_feature    9465..9482
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.71"
                     /tool="antismash"
     gene            complement(9534..9941)
                     /locus_tag="O3I_RS25895"
                     /old_locus_tag="O3I_025755"
     CDS             complement(9534..9941)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS25895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O3I_025755"
                     /product="hypothetical protein"
                     /protein_id="WP_237748133.1"
                     /transl_table=11
                     /translation="MNDHRLGTRNPYAGPGSSRSTKYQQDKPAPERRERTRAGNVERTR
                     VTRDHDTIRQWAERRGATPATMPGIAYGGPMGSLRLDFPGYGGAVLRPVDWDEWFATFE
                     ERHLHFRYQEQLPDGTPSNFNRLERSTRDER"
     misc_feature    complement(10083..10098)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
ORIGIN
        1 gctgggtttc tccggtgtcg gcggttcggt ggcgagttcg ctgcggaccc gcttccccgg
       61 ccggaccgtg cgcacggcct gtcgcgccgc gggcatcgcg cccgacgagc cggtcgggcc
      121 gatcgacccg cagcgctggc tcaccctcta tcgcggattg cgcggcaact ccgtccggga
      181 cagaaacgta tcggatacat ttgtgtatca gacaaggaag ggtggccggt gaccagcaac
      241 ttcggtgact accagttcga gatctacctg caggggctcg ccggggtgca gccgagcttc
      301 ccgatgaccc acagcgaact ggaagcccgc gcgcaggccg cgctgccgcc ggggatctgg
      361 tcctacgtgg cgggcggcgc gggcgacgaa ctgacccagc gcaccaacgt gacggccttc
      421 gagaagtacg gcatcgtgcc ccgcatgctg cgcgccacca aggtccgtga cctctcgatc
      481 gaactcttcg gcatgcggct gcccgcgccg gtcttcatgt ccccgatcgg cgtggtcggg
      541 ctctgcgccc aggacgggca cggcgatatc gcgaccgcga aagcggcggc gcgcaccggc
      601 gtgccgatgg tggcatccac cctgacagtc gatccgctcg aggaggtggc cggtcacttc
      661 ggcgacaccc ccggcatgtt ccagctctat acgcccacca atcgcgacct ggccgagagc
      721 ctgatccggc gcgcggaaca ggccgggttc aaagccattg tggtgacgct ggatacgtgg
      781 gtgaccggtt ggcggccacg cgatctgagc acctcgaact tcccgcagct gcgcgggcac
      841 tgcctggcga actacttcac cgatccggtg ttccgcggct tgctggcgaa gagtcccgaa
      901 gaggacccga agaccgcgat tctcacctgg gtcgcactgt tcggcggcgc gcccacctgg
      961 gacgacctgc cctggctgcg gtcgctcacg gatctgccgt tgatcctcaa gggcatctgt
     1021 catcccgagg acgcgcggcg cgccaaggac gcgggcatcg acggcatcta ttgttcgaat
     1081 cacggtggca ggcaagccaa tggcgggatc ccggcgatcg agcatctgcc cgagatcgtc
     1141 gaggccgtgg acggtttgcc ggtgctgttc gactccgggg tccgctccgg cgccgacatc
     1201 atcaaggcac tcgcgctcgg cgcgaccgcc gtcggcatcg gccgcccgta ctcgtacggt
     1261 ctcgcgatcg gcggggtgga cggcatcgtg cacgtgctgc gcacgctgct cgcggaggcg
     1321 gatctgatca tggcggtgga cggctacccg accatcgccg acctggccga cgacgcgatc
     1381 aggcgactgc actgatcggc ggcggccgag ttgtcctgcc gggcagggca actcggccgt
     1441 gccgcggtca gctcggctga atcagcgtgc gcttgaggat ctttccggtc gcgttgcgtg
     1501 ggagcgcgtc caggaaggtc acatcgcgcg gtaccgagaa acggctcaac cgatgccgga
     1561 tgtagttgca caccatgtcg cgatccagcc cgaaaccctc cctggtcacc acgaacgcgg
     1621 cgaggcgctg cccgtactcc tggtccggca cgccgaccac cgccacctcg ctgacctgcg
     1681 gcagatgcga gagcgcctct tcgaccggac gcgggaagac gttctcgccg ccggagatga
     1741 tcatctcgtc gtcgcggccc gcgacgaaga gcagcccgtc ggcgtcgagg tagccgaggt
     1801 caccggtatc gagcatgccg ccccattcgg tgggcggctt cgcgtcggtg tagccgtcga
     1861 acagcatgtg gttgagcacg aagatgcggc cggtcacccc gcgcggcacc ggacgcaatt
     1921 ctttatccag cacagcgagt ttcgtgccca gcggcgggcg tcccgcggtg gtcggcgcgg
     1981 cccgcagatc ggccggatcg gcgatggtcg cccaggacac ctcggtcgag ccgtacacgt
     2041 tgtagagcac gtcgccgaag gcgtcgagaa acttcaccac cgtcggaccg gcaaggggcg
     2101 caccgcaact ggcgaccacg cgcaggctcg acgtgtcgta gcgatcgcgc acgtgcgcgg
     2161 gcagatccag gatccggttc accatcaccg gcaccacgat caacgcggtg acccggtgct
     2221 cctcgatcaa ccgcaggcag tcctcggcgt cgaaccgctc cggcagcacc acactcgccc
     2281 ggatcggcgt gctgatctgc aacgcggcca gtccccaggt gtggaacagc ggcgcgggga
     2341 tgagcatcgt ctcgtccatc cgcatcggga tgcgcgagag cagcgccgcc acggtgccga
     2401 aacccttggg ctgcggtcgc tgtgcgctct tgggcgtgcc gctggtgccc gaggtcagca
     2461 cgatcagccg gccgggatgg ctgggccggg cgaaggtcgg cgcgtcgagc gcgatgaggt
     2521 cgtcgacggt gttgcggtcg taccgggtca ggtgtccggt cgcgaagcga ggtacggacg
     2581 cggccacata cttggtcagc ggctcgtact cgtcgtcgag gaagacggcg gacagtccgt
     2641 gccggtcggc gacgtcctcg atctgccggg ccgagaggcc ggtgttcagc agcaccgtgt
     2701 cgacgccgag tttgccggtg gcgaccatgc attcgaccat cgtgacccgg ttgcgcgaga
     2761 gtagccccac tttgtcccgc gcgccgatgc cgagcatcgc cagcgctgaa gcgagtttgt
     2821 gggtgcgatc gtgcatttga ccgaacgtgc gcgcggaaac gccgtccact accgcgagcc
     2881 gattaggcga atgccctgcg gcggccgcgt aaccacccgc gagcgtgaat ccccagcggg
     2941 cgagcccgcc cacctgtttg agtgcgcggt cgggccggta ggtgcgcacg acaccggtcg
     3001 tcaccatctg cttcagcgtg ccgacctgct gtttcgtcgg cgattcctcg ccgttgacga
     3061 gcaccgaggt cggcgtgccg aggaccgcgt cgacgatgcg gctcaggccg gccgtggtcc
     3121 aggcgatcac gtccgcgttg tcctgttcgg cgatccaggg atggatgcgt ccggcgccga
     3181 agtaggtgat ggtgacccgc gtcggctccg cctcggcttc cccgcgcagc cgcaccgcgg
     3241 cgaaactgcc caggccaggg cattgcagct cgaaactttc cagctccttg ccgaccacca
     3301 gccgcagcgc gggcacggtg agttcggtgt ccgcgtgccg cgcgcgaatc agcagcaccg
     3361 ggtgggtgtc cgaggtttcc gcgcgctcac agtcgccgat tccctggaac atgcgggcgt
     3421 aggtctgcgg gtccatgaaa aagtcccaca gtgcttttcg tgcggctgcg aactccgcgt
     3481 ctattgtgat gacatccgta accaactgcg tgcttttctg catgggccga ccgagcggtc
     3541 tgcgtcggca tggtcacgta gttgtaccca tcggtaacaa ttaggtcaag tacgcaacgc
     3601 attgccatcg attagccaat tagctggtca aatgcccatt ttcgtccgaa tccactatcg
     3661 gccacagcac agtgcacaat gttcgcgaaa tgcgcgcaca gttgcgaaaa atccgctatg
     3721 cgaccgagaa cgtcacgcgc gcaatgccgg tcgacctggg gttcggcatg cggcagccaa
     3781 gggtgcgcac gacagcatcc ccgccgacct tgtccggcgc accaagacag cgcagttggc
     3841 cgagattttg ccggggtcag aagttctttc ggcgcgtccg ggccggacac gagagccctc
     3901 ggcagtgcgg actacccccg cgatcacctg agtaaccact ggcccccgcg tcgaaacacg
     3961 ctcgcgcagc ggatcgtacc gccgatccgg cggccgcgcg cggcgagcgg aacccggccg
     4021 aaccgaaagg tgcccctcgc cccgcgggaa cgcggcgaag ggcaccttcg gtcgacgatc
     4081 ggcccaggtc aggcggacag cggcgtgaag tcgcggctgc cgatgtagcc gggccgcggc
     4141 gcgggcgcgc cgaacggctc gaccagggtg ttctccacgc tgttgaacac caggaagatg
     4201 ttcgagcgcg ggaacggagt gatgttgttc gacgaaccgt gcatcaggtt ggagtcgaac
     4261 agcagcgcgg agccggcccg tccggtgaac tggctgatgc cgtactggtt cgccagcgtc
     4321 gtgatgtcct cctgggtcgg cacgccgatc tcctgctcct gcaaggactc ccggtagtgc
     4381 tccgcgggcg tgctgcccaa gcacggtacg aacgtgcgat gcgagccggg catcaccatc
     4441 aggctgccgt tgatcgggta gttgtccgtc agcgcgatgg acaggctcac cgctcggggc
     4501 gccggcatgc cgtcctcggc gtgccaggtc tcgaaatcgg agtgccagta gaaacccgtg
     4561 ccgcggaagc ccggcatgta gttgacccgg ctctggtgga tgtacacctc ggaaccgagc
     4621 acctgccggg ccagcccggc gacccgggac tcgcggacca ggtccgcgat cgccgcgctg
     4681 agccggtgca cctcgaagac cgaccgcacc cggttcgacg ccttctccac gatcacgcgt
     4741 tcgtcgtcgc gcaacgcggg atcgcctgcc agcctgccga tctcggcgct gaaatcagcg
     4801 acctcggtgg gcgtgagcag ttcgtcgatg ctggcgaagc cgtcggtgtc gaaggccgcg
     4861 agcgccgggc tctcgacccg gccccacacc gtcggatcgg tacgatccag gtgcgtggcc
     4921 ggtgcatcga tccgggtcgg gtaacgatcg atccggtctg tcgagggtcg agtttcagtg
     4981 tgctgcaaaa ccattaccgc ctatcccccg atcgtcgcgg caacgagcgg gtacgcgccg
     5041 gtgctgtcgt ggacctcctg cccggtgacc ggaggattga acacgcacat catccgcatc
     5101 cgggtgcggg ccgagagctg gtggcgctcg ttcccgtcca gcagatacat cgagccggga
     5161 ccgagctcgt aggtcacgcc gttgtcgaga tcggtgagcg tgccctcacc ctcgatcaac
     5221 cataccgctt ccacatggta gcgatagtgg aactcgtgtg tgctgcccgc gtcgatggtg
     5281 gtctcgtgga aggagaagcc gaccccgtcg ccgccgagga tgatgcgctt gctgcgccaa
     5341 ccctccccgg ccacatcgcg ttcggtatcg gtgatctcgg cggtggtacg tacgatcatg
     5401 cgatcgcccc cattccattg caaacggtgt ccacggcgcc ggtgaggatg ctcaggccgt
     5461 ggtccagttc ctggtcggta atcgtcaacg cgggcagcaa cttcaccacc tcgtcggtgg
     5521 aacccgaggt ctccaccagc aagccgcgtt cgaacgcgat ctggcacacc ttgctcgcct
     5581 gcgacgggtc gtcgaacacc acgccgtgca ccaggccgcg gccgcgggtc gagacgccgg
     5641 ggacgttgcc cgccagttcc gtcagcgtac tcgcgatgcg ttcgcccttg gcgagcgtcg
     5701 cgttctgcag ggtgtcgtcg gaccagtagt gccgcaacgc gacatcggcg gtgacgaagg
     5761 ccgggttgtt accgcggaaa gtgccgttgt gctcgcccgg cgaccactga tcgagctcgg
     5821 acttgaacag caccagcgcc atcggcagcc cgtagccgcc gatcgacttc gacagcgtga
     5881 cgatatcggg ggtgatgccg gcgagctcga acgagaagaa cggcccggtg cggccgcagc
     5941 ccatctgcac gtcgtcgacg atcagcagga tgtcgcgcgc ctcgcagagc ccggcgaggt
     6001 gccgaagcca ttccgcgcga gcgacattca cgccgccctc gccctgcacc gtctccacga
     6061 tcacggccgc cgggcggtcg aagcccgagg aggtgtcgtc gagcaccttc tccatccact
     6121 ggaaatcggc ggtggtgccg tcgaagtagc cgtcgtaggg catgtgcgcc gcgtgcacca
     6181 gcggcacacc ggcgccagcg cgcttggccg cgttgccggt gaccgagagc gcgccgaggg
     6241 tcatgccgtg gaaggcgttg gtgaagctga cgatcgtctc ccggccggtc accttgcggg
     6301 ccagcttgag cgcggcctcg accgcgttgg caccggtcgg accggggaac tgcaccttgt
     6361 agtccagccc gcgcggcgtg aacacggtgt cgcgcaaggt ctccagcagg ctgcgcttgg
     6421 ccgaggtgga catgtcgagc ccgtgcgtga tgccgtcgct ggcgatgtag tccagcagcg
     6481 gctgcttgag cacgtcgttg ttgtggccat agttcagcgc gcccgcgcct gcgaagaagt
     6541 cgaggtagtc cttaccgtcc tcgtctcgta accagctgcc cttggccttc gagaagaccg
     6601 caggccaggc gcggcagtag ccgcgcacat tcgattccag actttcgaaa atgctcgtgt
     6661 cggcggtgat catcggtggt cctcctgcgt tgtgcgagcg atcggtgcga tgagatacag
     6721 gtcctcggac tcatggctgt ccgggaagac gcccttgtcg aacaaggggc gcttggtcat
     6781 tcgggcgtca cgctgccggg cgaccgagcc gaacagcgcg atggacgcgg cgttgtcggg
     6841 tgagatcgtc gtctccagcg tcgtaacacc ctggggtgca acagtgttca gcaaagcgtg
     6901 cagcaattgg gcgccggtgc cccggccgcg ctgcgctcgg tccaccgcca cctgccagac
     6961 gaacacggtg tccggcgcct gcgggcggag gtagccgatc acgaaaccga ctacacggcc
     7021 ctgtagttcg gctaccaccg tggtctccgc gaagtcgcgg caccacagca ggtaggcgta
     7081 gctcgaattg acgtcgagga cctcggagtt cttcgcgatc cgccacagct gggcgccgtc
     7141 ctcgacgcgt ggcttgcgca gaaccaactc ggtccggtgg cggccggggt ccggacccag
     7201 cgcggccgcg gcctgctcga cacgggatcg ttcgatctcc gcggtgttca acgttggcac
     7261 tgacatacaa ctccctggtc gggcctgtag tacttatcca ggcttacgaa tcaactttga
     7321 gctgacctgg tcgagttcat tacggttgtg ttacggctcg attgcgccga cgcccggcaa
     7381 ggtggcgacg aaccgggcgc cgccgcccgg gcggctcaca cattcgacct ggccaccgtg
     7441 cttggcgacg gtctcggcga ccagcgcgag gccgatcccg gtcccggtcg ccgaccgccg
     7501 gccgctgcgc gcggtcgcga accgttcgaa gatgcgcacc cggtctgccg tcggcacccc
     7561 ggggcccgca tcgtccacga cgatcaccgc cgcgtcgccc acccgatccg cggcgaccgc
     7621 gaccacgccg ccgccgtgca gatccgcgtt ctgcagcagg ttcaccaccg cccgctccaa
     7681 ttcgagcttg cggccgagca cgacgacatc ctcgtgcacg gtcagcagct cgggcgggta
     7741 gcggcgctcg gcgagcgtgt gccgcaacag atctcgcacc gacagcggtt cctggtcgcc
     7801 gcggtgcatg cccgcctcga cccgcgcgag cgcgagcagg tcgtcgagca accgccgcag
     7861 atgatcgacc tcctcggtga ccagcgcgag cgcccgctgg gaacgctccg gcagatcgtc
     7921 gcgatgccgg ttgagcacgt ccacactggc catcaaagtg gtgagcggcg tgcgcaattc
     7981 gtgactgaca tcgccgaaca gccggtgctc gcgctcgatt cgccgttgca gcgagtcgac
     8041 catggagttg aacgactcca cggtggtcgc cagatcctga tcgtgggtgg cggacaggcg
     8101 cagatccaga tcgcccgagg cgatccgggc ggcggtcgcg gccagctggt gcagcggagt
     8161 gagcacgcgc ctgctcgccc accagcccac ggccgcgccg atcgcggtga ccgcgaccgc
     8221 gcaggcgacc agcacattgc gcagcagatc caggttcgcc tgcaggtcgg tggtaccggc
     8281 ctgcgcgcgt tccagatagc tgcgactgat ggtgaacacc gagaccacca gcaccagcga
     8341 catcagcgcg ctgatcgtgg tgaacacggc ggtcacccgg ccgcgcagac tccagcgtgt
     8401 cggatcgagc cagcgttcag cgctggacat cgagtcgata gccgagcccg cgcaccgtca
     8461 ccacgacgcg cgggtcggaa gcgtctcgtt cgatcttcgt gcgcagcctg cgcatgtgca
     8521 cgtcgacgat ccgctcgtca ccgaaaaagc cttggtccca tactcgttcg agcaggacgg
     8581 tgcggctgag cacccggccc ggcgtctccg cgagttcgca cagcaggcgg aactcggtga
     8641 gcgtcagccg gatctcctcc tcgccgcggc gcaccacgcc cgcgtcctgc gcgagcacca
     8701 gcggcgcctc ggggtcggcg tcgagcacga cttcctgcgg cggcgcctca cgttcggcgg
     8761 taagcctggc ccggcgccgc accgcccgca tccgcgcggt gatctccttg atctcaaacg
     8821 gcttcgccac gaaatcgtcg gcgcccgctt ccagcgcggc gaccacgtcg tgggtgtcgt
     8881 cgcgcgcgct gaccacgatg atcggcacgt cgtgttcgcg gcggatctcc cggatgcagt
     8941 cgaagccgtc catatcgccg agcatcagat cgacgatcat cacatcgggc acgccgttgc
     9001 tgcgcagatg ggtgagcgcg tcctcggcct cctcggcctc ggcgacgtcg tagccttcgt
     9061 cctccatggc gagccgcagc gcaccccgga cccggtcgtc gtcctcgacg atcatcaggt
     9121 tcgccgtcat cgccgcacct cgccgacgct cggccgcgct aaggccgcga aggcggctgt
     9181 gcctctggtt cgctcgctac gctcgctcaa atacctatcc tttgcagaca cgcaggacgt
     9241 ctgccggtgc ggagaatcgc atccccatac cgacgcgaca taccgcttgt tcgagccacc
     9301 ttgccgatct atcggatacc cgaacagacc attcggtaaa cgccgtgtgg acgcttgatc
     9361 agcccttgca ccaccccgca ccggccgcaa cgggtgcggt gtgcgggtcg gacgcgggcg
     9421 gctcacgcct gtctggttat ccatccaccc tggccgcatg agcgtcgtta tgcaaccgtt
     9481 acgtgatgcc cgacacacgg gttgccccgc gttttcgcgt ctgcccggcc ggttcagcgt
     9541 tcgtcgcgag tcgaacgttc cagccggttg aagttgctcg gtgtgccgtc cgggagctgc
     9601 tcctggtagc gaaagtgcag gtgccgttcc tcgaaggtgg cgaaccactc gtcccagtcc
     9661 accggccgca gcaccgctcc gccgtagccg gggaaatcca ggcgcagcga acccatgggt
     9721 ccgccgtacg cgatgccggg catcgtcgcg ggcgtggcgc cgcgccgctc cgcccactgc
     9781 cggatggtgt cgtgatccct ggtgacccgg gtgcgctcca cattccccgc gcgggtgcgc
     9841 tcccgccgct cgggtgcggg cttgtcctgc tgatacttcg tcgacctcga gctccccggt
     9901 ccggcgtacg gattccgggt gcctagtcgg tggtcgttca tgacgatctc ctgagccatc
     9961 ggcatcgtaa gtccggttcc ctttcacgta cccgacgcca acttcgcgca aaccggcact
    10021 acgtcacaaa gcccccgcca acgctgctga aacgcaccaa aaacgctgac cgacagccac
    10081 tctgtgagcg gagtcaccgg tagccacgcg gtggttgggt ccggccacgg tgggtaccgc
    10141 tgctgcgagg aggcaatccc atgaccactc cagtaccgca cgaaccgagt ccgattcccc
    10201 cgatcccgga gcccgatccg gtgccgccgg tgcccggtcc ggacccggcg cccgcgccgc
    10261 cgcagcccgg gccggatccg ctgccaccgg tacccgaacc acgcccggcg cccgacccgg
    10321 gccgcccgct gccgccggta cccgaaccgg gcgggccgct gtccccgccg cacgatccga
    10381 cgccgccgaa tccgctctg
//
