LOCUS       NC_018681              10972 bp    DNA     circular CON 21-MAR-2024
DEFINITION  Nocardia brasiliensis ATCC 700358, complete sequence.
ACCESSION   NC_018681
VERSION     NC_018681.1
KEYWORDS    RefSeq.
SOURCE      Nocardia brasiliensis ATCC 700358
  ORGANISM  Nocardia brasiliensis ATCC 700358
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Complete genome sequence of Nocardia brasiliensis HUJEG-1
  JOURNAL   J Bacteriol 194 (10), 2761-2762 (2012)
   PUBMED   22535940
REFERENCE   2  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
REFERENCE   3  (bases 1 to 9436348)
  AUTHORS   Vera-Cabrera,L., Ortiz-Lopez,R., Elizondo-Gonzalez,R.,
            Perez-Maya,A.A. and Ocampo-Candiani,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2012) Servicio de Dermatologia,Hospital
            Universitario 'Dr. Jose E. Gonzalez' and Centro de Investigacion y
            Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo
            Leon, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, Nuevo Leon
            64460, Mexico
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000250675.2-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 01:53:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,551
            CDSs (total)                      :: 8,489
            Genes (coding)                    :: 8,417
            CDSs (with protein)               :: 8,417
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 13 of 72
            Pseudo Genes (incomplete)         :: 63 of 72
            Pseudo Genes (internal stop)      :: 3 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:09:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2071782
            Orig. end                         :: 2082754
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003876.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="10"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5972
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    655..666
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.25"
                     /tool="antismash"
     misc_feature    complement(766..779)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            2709..2978
                     /locus_tag="O3I_RS09205"
                     /old_locus_tag="O3I_009080"
     CDS             2709..2978
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_PP (5-58). E-value: 4.3e-08. Score:
                     25.4. Matches aSDomain:
                     nrpspksdomains_O3I_RS09205_PKS_PP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349689.1"
                     /locus_tag="O3I_RS09205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009080"
                     /product="acyl carrier protein"
                     /protein_id="WP_014982634.1"
                     /sec_met_domain="PP-binding (E-value: 9.8e-07, bitscore:
                     28.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVADAAVAERVRGLIQRMAPDAQATAEDSQRLIEDLGFDSLRL
                     MELTVVLERSFDLPRYRPEQLVGVRRVGEVVTLVTGTLAAEGRA"
     aSDomain        2724..2882
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O3I_RS09205_PKS_PP.1"
                     /evalue="4.30E-08"
                     /label="O3I_RS09205_PKS_PP.1"
                     /locus_tag="O3I_RS09205"
                     /protein_end="58"
                     /protein_start="5"
                     /score="25.4"
                     /tool="antismash"
                     /translation="DAAVAERVRGLIQRMAPDAQATAEDSQRLIEDLGFDSLRLMELTV
                     VLERSFDL"
     misc_feature    complement(2734..2748)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            2975..4066
                     /locus_tag="O3I_RS09210"
                     /old_locus_tag="O3I_009085"
     CDS             2975..4066
                     /NRPS_PKS="Domain: NAD_binding_4 (8-229). E-value: 1.5e-48.
                     Score: 157.0. Matches aSDomain:
                     nrpspksdomains_O3I_RS09210_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349690.1"
                     /locus_tag="O3I_RS09210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009085"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_014982635.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.3e-14, bitscore:
                     53.0, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 2.1e-46, bitscore:
                     157.0, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGRDTVLLTGASGLVGAEVLARLSAASRPVAAVLHSNSRIIRND
                     GTVLGAGRAVAGDIRAPGFGLSEPDRADLADRVGIIVHSAATTAFDASASDYDELNVQG
                     TANAIELARAWDVPYVHVSTAYVCGERSGTVGEHDLDVGQSFGNGYEESKFRAEQLVRA
                     AGEQGLRWAIVRPGIVTGASGTGVIREYKNLYTVVKLMVEGKLRSLPGRYDATLALAPV
                     DHVADVIAAAVLDFDSAHGRTFHAVGRDALSLREVSDVLAEYPSFEVATFVPAGTFDEN
                     DLDGIEREYYRRIGVLYTSYFRRKLRFATANAEVLLGKPAPASGKEYLRLLLDYCLESG
                     YLGVPLPSIEEVLAGNDFGGADR"
     aSDomain        2999..3661
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O3I_RS09210_NAD_binding_4.1"
                     /evalue="1.50E-48"
                     /label="O3I_RS09210_NAD_binding_4.1"
                     /locus_tag="O3I_RS09210"
                     /protein_end="229"
                     /protein_start="8"
                     /score="157.0"
                     /tool="antismash"
                     /translation="LTGASGLVGAEVLARLSAASRPVAAVLHSNSRIIRNDGTVLGAGR
                     AVAGDIRAPGFGLSEPDRADLADRVGIIVHSAATTAFDASASDYDELNVQGTANAIELA
                     RAWDVPYVHVSTAYVCGERSGTVGEHDLDVGQSFGNGYEESKFRAEQLVRAAGEQGLRW
                     AIVRPGIVTGASGTGVIREYKNLYTVVKLMVEGKLRSLPGRYDATLALAPVDHVADVI"
     gene            4063..4983
                     /locus_tag="O3I_RS09215"
                     /old_locus_tag="O3I_009090"
     CDS             4063..4983
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349691.1"
                     /locus_tag="O3I_RS09215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009090"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_014982636.1"
                     /transl_table=11
                     /translation="MSTSLEQLLAGLTPQPEKVTAYAADTYLLETVDQLDRLGVDAAKF
                     AREHSLLLLKPDAIVARAVGPTLKWLAGNDFQVVAAFRVAVDRHLARALWYFAWNIASP
                     ERRTLADLLVGISDVLVLVVRGEDGQLPVSVRLTEAKGPTDPRKREAGQLRYLLGRHNY
                     LLNLVHSPDDPADVLRELAIYLGEQRRAAVIAQAGTGEDRSAEALALTNGLYTQVPARS
                     FDRADATEQILRDLARAGAPAPDDFDPQADDECARLLYAAWADGRELDPWSVIVLGSYV
                     LPMRAGTQQQTLRPVSAEDWQEARP"
     gene            5001..5972
                     /locus_tag="O3I_RS09220"
                     /old_locus_tag="O3I_009095"
     CDS             5001..5972
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349692.1"
                     /locus_tag="O3I_RS09220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009095"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_237748274.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-18, bitscore: 66.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTATHLRTISRELAHRCAVSEVFVTSLDAVAEHEFVAGAQLPRM
                     HAYYSDHTGTLGLRHDPLLVMEAARQAAIALSHEFYGVPAEMGFIVRTFNGTGADTAAW
                     TVGTAPADLVMRVRVPRRHVRGDTLHGLDMVLEIDCGGESMMTVDGSFSWVTPRQWAAL
                     RSAFRTSLELGPYVGASALGDRAAAAVVGRENWRNVVIGPPQLDGNTARATLVPDLGHP
                     FLFDHELDHVPGSLLIEACRQTALAMVLHRAPRLECVGSSFERFVELDRPAECVAEITG
                     QQADRTVIHCEIRQAGAVAAEIDLEFADDELAFGAEQPGAGR"
     misc_feature    5124..5126
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    5748..5750
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5969..6580
                     /locus_tag="O3I_RS09225"
                     /old_locus_tag="O3I_009100"
     CDS             5969..6580
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349693.1"
                     /locus_tag="O3I_RS09225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009100"
                     /product="nitroreductase family protein"
                     /protein_id="WP_014982638.1"
                     /sec_met_domain="PF00881 (E-value: 2.3e-27, bitscore: 95.1,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTDLLTTTRSTRRRLDLSRPVDRADLLACLDIAVQAPSGSNRQ
                     PWRFLIVQDGETKRQIGEFYRKSFAANMSGRTPRPDQLGDLASGQYLADHLAEVPALVF
                     VCSLGRPPEPASPPRLASFYGSIYPAVWNFMLALRERGLGSCLTTAHLAYEREIAELLG
                     IPYDEVTQVAMVPVAHLLPGRDRPGRRTPAAELTSWDRWA"
     gene            6577..7491
                     /locus_tag="O3I_RS09230"
                     /old_locus_tag="O3I_009105"
     CDS             6577..7491
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349694.1"
                     /locus_tag="O3I_RS09230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009105"
                     /product="hypothetical protein"
                     /protein_id="WP_237748275.1"
                     /transl_table=11
                     /translation="MTNPLVSVARIARFTAVMYKPHYLLYGVLWVLALEGTASLVTQPD
                     SAWRPTGATLVRIVVVAFVLLYLRMVDEQKDLEYDRVHNPDRPLVTGAVSAVELRIGMG
                     VIAVGAIAGSLALSVGSAVMIAVAMAYGLALWGLERAFRPVRDAIMVNLLVTYPVQLLV
                     TGYVVVSAIDTGEVRAHWQAAAVAVIFAGAFLQFEFARKTSRERRPGEMLYSNALGANG
                     SVAAMLLCAVVAVLCDILLVRPWDFAAPGSLIACVPVVLLVLPGFGAWKFLRSARIDYP
                     FGPAVLFILTLYLTLVAQALILP"
     gene            7593..7937
                     /locus_tag="O3I_RS09235"
                     /old_locus_tag="O3I_009110"
     CDS             7593..7937
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS09235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O3I_009110"
                     /product="hypothetical protein"
                     /protein_id="WP_014982640.1"
                     /transl_table=11
                     /translation="MRTLLWRIGGGGCLVTLASGLLIAGSGSAQADPDACYVEPGITDA
                     VARCHDGAGASALEVECLGLMTPSTPDGPIFGAYSGTESVPSAVGQPMRASCISDDTLG
                     ITTRAFVTPR"
     gene            complement(7944..8708)
                     /locus_tag="O3I_RS09240"
                     /old_locus_tag="O3I_009115"
     CDS             complement(7944..8708)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF010309.0"
                     /locus_tag="O3I_RS09240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009115"
                     /product="aminoglycoside adenylyltransferase family
                     protein"
                     /protein_id="WP_041562504.1"
                     /transl_table=11
                     /translation="MTQEDGVAGLVRRVFGEQLVGVYRHGSAVLGGLRRYSDIDVLVVS
                     ADSLDGAQRRVLLEGLLELSGGVGGALRPVELTSVVQGAVRPWRYPPTSDFQYGEWLRA
                     EYEGGEIPSATVSPDLAVLITMVLDGDSTLVGPPPARVLDPVPRADLTHAMVAGVPELV
                     EELGTDTRNVLLTLARVWMTVATGVIAAKDKAADWALDRIAQEHRPVLAHARAVYLGAA
                     EENWTGQAARIERAALTLRAGIEQAATGNETS"
     gene            complement(8752..9189)
                     /locus_tag="O3I_RS09245"
                     /old_locus_tag="O3I_009120"
     CDS             complement(8752..9189)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O3I_RS09245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O3I_009120"
                     /product="hypothetical protein"
                     /protein_id="WP_014982642.1"
                     /transl_table=11
                     /translation="MLVLFADPLSDLWYRHLYAWLQSRETRDEIEEPMTTTITDEQIQQ
                     LAASAKPYSVAILHWGPQRHMDGAAAIELEHQRRMVTLRADGVIAILTPVVSDTVAGIA
                     IMTVTPEKAKEIMDGDPCVQAQMMHCEVHPCLSFPGDTLPA"
     misc_feature    complement(9127..9129)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9190..10011)
                     /locus_tag="O3I_RS09250"
                     /old_locus_tag="O3I_009125"
     CDS             complement(9190..10011)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072603.1"
                     /locus_tag="O3I_RS09250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009125"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_014982643.1"
                     /transl_table=11
                     /translation="MPTIPPGQSPAPQLESHRARAIAESFGNDAARYDRCRPHYPAELV
                     AAVIDAIPGRSVLDIGAGTGIVTRQFQAAGCTVLGVEPDTRMAEFARHRGAEVEVSTFE
                     TWDPAGRSFDAAVAGQTWHWVDPVAGAHKAAAALRPGGTVALFWNVQQPPPELEQALAE
                     VFRRVVPESPIAHLPNVSSVEIYTAMCDKAADGIRGTAAFDEPQQQRFEWTQRYTRDEW
                     LDYSATTGATTRLAPAVLDEVLAELGAVIDRTGGDFVCRYTTVMVTATRVA"
     gene            10099..10674
                     /locus_tag="O3I_RS09255"
                     /old_locus_tag="O3I_009130"
     CDS             10099..10674
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925917.1"
                     /locus_tag="O3I_RS09255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O3I_009130"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_237748276.1"
                     /transl_table=11
                     /translation="MRDVRAQLFDAAERIMRRDGPSALTSRAVTTEAGCAKGVLHRHFA
                     DFDDFLAELVLDRVGKLEQLSAALYVSAGTGSVVENLSDALTSIFESVVVAIVGLVTFR
                     DELRARLRHRWPTGLPLMSEATAMLAEYLAAERVHGRIAKNADIDALAPALIGTGHLLF
                     ADRTGPLPDTAAVIRVVTTVIGGALTAE"
ORIGIN
        1 cagcattccc cgtcgccttc gttcgcgctc ggctatgtgg cacatcgggt ttcgcccgcc
       61 gagatcgccg atctggacct gtccggttgg cgcacgctgg ccgtgggatc cgaaccagtc
      121 gaggtggccg atctccagtc cttcgccgaa ctcgccggtc cgcaaggctt ttcgatgagc
      181 gcctacacac tcgcgtacgg gttggcggag tcgacgctga tggtgacctc ctcggcgcgc
      241 catcgcccga tcaccgcgct gcggatcgat actccgacac tgcgtttcgg tgagccggtc
      301 accgtactgg aggagcgcct gctcgacgat acccaccgcg tcgagggcgc gggctggatc
      361 accgggctcg gcttctcgac cccggaatcg accgtcacgg tggtcgacga cgaaggccgt
      421 ccgctgccgg acggcacgct cggcgagatg gtggtgatcg gcgattcggt cgcgctgggc
      481 tactccgacg aatccgagtc cgcgtccacc cggatcctcg acggcgcgct gcattccggc
      541 gacgccgggt tcctgcaccg cggtgaggtt ttcgtgctcg gccggatggg gtcgagcctg
      601 aaggtgcgcg ggcgctcggt gttcatggag gacatcgaat cccgggtcgc ccaggaaacc
      661 ggtatcacca agggcaagct ggccgcggtc gcgatcaccg agcccggtgc ccagggcatc
      721 gcactgttcg ccgagtcgaa gccgggtgac tggatcgccg aggctcgcaa gatcatgcgc
      781 agcgagctcg gtccggcgca gacggtcacc gtcgtcaccg gcccgcgcgg catcatccgc
      841 cgcacctcca gtggtaaacc acgccgcagg tacatgtggg agcagttccg cggcggtgcg
      901 ctgaccgacg cggtcgcgca cgaagagcag gtcggcgccg ccgaatccgg ggcggcccgc
      961 gggacagccg gctcggacct gccggtgccg accatggccc tcgaccgggc ggtgcaactg
     1021 ctggacaagg cgctggacag cgttgccgtg ccccgggatt cgacggtgct gttcgagggc
     1081 tcgctggccg agggcttcgg caacgacggg tccgatgtgg acttcctggt gctcgcgccc
     1141 ggtgacgacg aactgcccac gctgccgacg gtgctgttca tcgacggccg ccgggtggag
     1201 gtgcggaccc gctcggccgc ccaactgcgc aggcagctcg aacaagtcgc gcacgccacc
     1261 agcgtgcccg acctggacga ggacctgctc aatcgatgtc agcggttcct gcgggccacc
     1321 gtggtccggg ccggcagcca ggacgtcggc gaattgcggg cgctgctccc gcatgccgcg
     1381 ttcacggcgc tgctcgccga atggtggcgc gaccgcgccc ggcacgcgct gcgatacgcc
     1441 gtcgcgttgc gggcgctgca cgcccgcgac gaagcggtcg agtgggcccg cgacggtttg
     1501 ctccaggcga tgaagggctg ggcggcgcgc cggggtgaaa gctacgtcga gaccaaatgg
     1561 ctgccgcgcc agttggatcg gatcggtgcc gacgaactcg tcgaccgcta ccgtgctctc
     1621 gccgatccgg ctgcctgggc gggcgagggc agcggccgca atgacgggga tgtgcgactc
     1681 gacgccccgg cactgtccga ggacgcgacc tgggcccagt tgtgcacgct ggctggagaa
     1741 ctcggcgtga ccggcgtcgt cgatgatccc gaccagatcc tgttcgcccg gatgcctgag
     1801 gtcaccacct ggcagatcgg cccgcgcgtg cacgtcatgc gggacagcag tgcggttttc
     1861 gtgctctcgg acgacgcggc gcgggcttgg cgttcggtag tgttccggca ttcgatcgcc
     1921 gcggtgctcg gtcgcgccca gcgcgacagc accgcggcct tggccgagtt cctgcgtctc
     1981 ggcttcgtcg gcctgcaatg gcgcggtggt gaagtgctca cgcccgcttt ggctctctgt
     2041 aaacgggcca aaccgtacac accggtgccg tccgcgatat cgcccgcgct cggcgtgacc
     2101 ggggcggtcc gcgagggggc gatcgcgacg ctctcgccgc tgcccgcgcg ccggttcacc
     2161 gagtgtgcgc tgaatctggt gtggtcgaac atcgtgctgg agaacgccag ggaggacctg
     2221 gcgggcgcga tcaagaacgc gcagggcgcg gtcgccgaga tcgccgcgca ccggatgatc
     2281 gcgatggccg tgcgagtggt gttgtccgcc tacggtattc acccgctgcc tgccgacgtc
     2341 gcgccgtcgt ccacgctggc ccggctgctg ccgccggctt cgccgcgccg ggacgatctg
     2401 ctcgcacgcc tggaatcggc ccggcgggta cggttcgcgc ggctcgtgga cggcggcgac
     2461 acgctggccg ggctcgccgt cgtggacgag ttcgtcgcgc tggtacgttc ggtggccgcg
     2521 gacccggccg ctaccggctt ccccgcctcg ttcgactcgc gggagcagtg gcgtcgcacg
     2581 ctggcgatcg gctacgactg gctgcgcatc gccggctatc tgaacaccga actgcccctg
     2641 gacgaggccc gcgacctgct ggcttccggt gggcagcaac cgcatctacg cgacgaggag
     2701 gtgcaggcat gaccgccgtg gccgacgccg ccgtggccga acgggtccgt gggctgatcc
     2761 agcggatggc cccggacgcg caggcaaccg ccgaggacag tcagcggctc atcgaggacc
     2821 tgggcttcga ctcgttgcgt ctgatggagc tgaccgtggt gctcgagcga tccttcgacc
     2881 tgccccgcta ccggcccgag cagctcgtcg gcgtacgtcg cgtcggcgag gtcgtcacgc
     2941 tggtcaccgg gacattggct gcggagggac gcgcgtgacc gggcgggaca cggtattgct
     3001 caccggtgcc tcgggactgg tcggtgcgga agtcctcgcc cggctgtccg cggcgagccg
     3061 tccggttgct gcggtgctgc acagcaattc gcggatcatc cgcaacgacg gcactgtgct
     3121 cggcgcgggc cgcgcggtag ccggtgacat ccgggcgccg ggattcggtc tgtccgagcc
     3181 ggatcgggcc gatctggccg accgcgtcgg catcatcgtg cactcggcgg cgacgaccgc
     3241 gttcgatgcc tcggcgagcg actacgacga gctgaacgtg caaggcaccg ccaacgcgat
     3301 cgagctcgcc cgcgcctggg acgtcccgta cgtccacgtc agcacggcct acgtgtgcgg
     3361 cgaacgcagc ggcaccgtcg gcgagcacga cctcgatgtc gggcagtcgt tcggaaacgg
     3421 ctacgaggag agcaagttcc gggccgaaca gctggtgcgg gccgcggggg agcaggggct
     3481 gcgctgggcg atcgtgcggc cgggcatcgt caccggcgcg agcggcaccg gcgtgatccg
     3541 cgaatacaag aacctctaca ccgtcgtcaa actcatggtc gaaggcaagc tgcgctcgct
     3601 gcccggccgg tacgacgcga cgctcgcgct ggcaccggtc gatcatgtcg ccgacgtgat
     3661 cgccgccgcc gtactggatt tcgattccgc gcacgggcgc accttccacg ccgtcggccg
     3721 ggacgcgctg tcactgcgtg aggtctccga cgtgctggcc gagtacccct ccttcgaggt
     3781 ggcgacgttc gtgcccgccg gcacgttcga cgagaacgat ctcgacggca tcgagcgcga
     3841 gtactaccgg cgcatcggtg tgctgtacac cagctatttc cgccgcaagt tgcgcttcgc
     3901 cacggcgaat gccgaagtgc tgctgggtaa acccgccccc gcgagtggta aggagtattt
     3961 gcgcttgctg ttggactact gcctggaatc cggttacctg ggcgtgccgc tgccttcgat
     4021 cgaagaagtg ctggcgggca acgacttcgg cggggcggac cgatgagcac gtcgctggaa
     4081 caactgctcg ccgggctgac gccgcagccg gagaaggtga cggcctacgc cgcagacacc
     4141 tacctgttgg agacggtcga tcagctggac cggctcggcg tggacgccgc gaagttcgcc
     4201 cgcgagcatt cgctgctgtt gctaaagccc gacgcgatcg tcgccagggc ggtcggcccc
     4261 accctgaaat ggctggccgg caacgacttc caggtcgtcg ccgcgttccg ggtcgcggtg
     4321 gaccggcatc tcgcccgcgc gctgtggtac ttcgcctgga atatcgcctc gcccgaacgc
     4381 cggacgctgg ccgacctgtt ggtcggcatc tcggacgtgc tggtgctcgt cgtccggggc
     4441 gaggacgggc agttgccggt ctcggtgcgc ctcaccgagg ccaaagggcc gacggatccg
     4501 cgtaaacgcg aggccggtca gctgcgatat ctcctcggta gacacaatta cctactcaac
     4561 ctggtgcatt cgcccgacga tcccgccgac gtgctgcggg agctcgccat ctacctgggc
     4621 gagcagcggc gggcggcggt gatcgcgcag gcggggacgg gcgaggatcg ctcggccgag
     4681 gcgctcgcgc tcaccaacgg gctctacacg caggtccccg cccgcagttt cgatcgcgcg
     4741 gacgcgaccg agcagatcct gcgtgacctc gcacgggccg gtgcgccggc ccccgacgac
     4801 ttcgacccac aggcggacga cgagtgtgcg cggctgctgt atgccgcgtg ggccgatggc
     4861 cgagaactcg atccgtggtc ggtgatcgtg ctcgggtcct atgtactccc gatgcgggcc
     4921 ggcacccagc agcagacgct gcgaccggta tccgccgaag actggcagga ggcacgaccg
     4981 tgacgccaaa tgtggaaacg gtgaaaacgg caacccacct acgcaccatc tcgcgcgagc
     5041 tcgcgcatcg gtgtgcggtg tccgaggtgt tcgtcacctc gctggacgcc gtcgccgagc
     5101 acgagttcgt ggccggtgcc caattaccgc ggatgcacgc gtattacagc gaccacaccg
     5161 ggacgctggg gttgcgccac gatccgctgc tggtgatgga ggcggccaga caggccgcga
     5221 tcgcgctcag ccacgagttc tacggcgtcc ccgccgaaat ggggttcatc gtgcgcacgt
     5281 tcaacggcac cggcgcggac accgcggcgt ggacggtggg caccgcgccc gccgacctcg
     5341 tcatgcgggt gcgggtgccg cgcaggcacg tgcgcggcga cacgttgcac ggcctggaca
     5401 tggtgctcga aatcgactgc ggcggtgagt cgatgatgac cgtggacgga tcgttctcct
     5461 gggtgacgcc gcggcagtgg gcggcgctgc gctccgcctt ccgcacgagc ctcgagctcg
     5521 gaccgtatgt cggtgcctcg gcgctgggtg atcgcgccgc cgccgcggtt gtcggccggg
     5581 agaattggcg caacgtcgtg atcggaccgc cgcagctgga cggcaatacc gctcgcgcga
     5641 cgctcgtccc ggacctgggc cacccgttcc tgttcgatca cgagttggac cacgtgccgg
     5701 gcagcctgct gatcgaggcg tgcaggcaga ccgcgttggc gatggtgtta caccgcgcgc
     5761 cgcgattgga gtgcgtcgga agctctttcg agcggttcgt cgagttggac cggcccgcgg
     5821 aatgcgtcgc ggagatcacc ggacagcagg ccgatcgcac cgtgatccat tgcgagatcc
     5881 ggcaggccgg tgccgtggcg gccgagatcg atctcgaatt cgccgacgac gagctggctt
     5941 tcggcgccga gcaaccgggg gcgggccgtt gaccggtacc gacctgctca cgacgactcg
     6001 gtccacccgc cgccgcctcg acctgtcccg tccggtcgat cgcgcagatc tgctggcgtg
     6061 cttggacatc gcggtgcagg cgccgagcgg cagtaatcgg caaccgtggc gattcctcat
     6121 cgtgcaggac ggcgagacca agcggcagat cggggagttc taccgcaaaa gcttcgccgc
     6181 caacatgtcc ggccggacac cgcggcccga ccagctcggc gatctcgcgt ccggtcagta
     6241 tctcgccgat catctggcgg aggtacccgc gctggtgttc gtctgctcgc tcggccgtcc
     6301 gcccgaaccc gcctcgccgc cgcggctggc gagcttctac ggatcgatct acccggcggt
     6361 gtggaacttc atgctcgcct tgcgggaacg cgggctcgga tcctgcctga cgacggcgca
     6421 cctggcgtac gagcgggaaa tcgcggagct gctggggatt ccgtacgacg aggtcaccca
     6481 ggtggccatg gtgccggtcg cgcacctgct gcccgggcgg gaccggccgg gccggcgcac
     6541 gcctgccgcc gagctgacca gctgggatcg gtgggcgtga ccaacccact ggtctcggtc
     6601 gcgcggatag cgcggttcac cgcggtgatg tacaagccgc actacctgct gtacggcgtg
     6661 ctgtgggtgc tggccttgga gggcacggct tcgctggtga cacagccgga ttcggcatgg
     6721 cggccgaccg gtgccaccct ggtccggatc gtcgtggtcg cgttcgtgct gctgtacctg
     6781 cgcatggtcg acgagcagaa ggacctcgag tacgaccggg tgcacaatcc ggaccggccg
     6841 ctggtgaccg gagcggtgag cgccgtggaa ctgcggatcg gcatgggcgt catcgcggtc
     6901 ggcgcgatcg cgggcagcct ggcgctgtcc gtcggctcgg ccgtgatgat cgcggtggca
     6961 atggcttacg ggctggcact gtgggggctg gaacgcgcgt tccgcccggt gcgcgacgcc
     7021 atcatggtga acctgctcgt gacctatccg gtgcaactgc tcgtcaccgg ttacgtggtg
     7081 gtctcggcca tcgacaccgg cgaggtgcgc gcacactggc aggccgcggc ggtcgcggtc
     7141 atcttcgcgg gcgcgttcct gcaattcgag ttcgccagga agacttcgcg ggaacgtcgt
     7201 ccgggcgaga tgctgtactc gaatgcgctg ggcgccaacg ggagtgtcgc ggcgatgctg
     7261 ttgtgcgcgg tcgtcgcggt gctgtgcgat atcttgctgg tccggccatg ggatttcgcg
     7321 gcacccgggt cgctgatcgc gtgtgtgccg gtggttttgc tggtgctgcc cgggttcggc
     7381 gcgtggaagt tcctgcgctc ggcgcggatc gactatccgt tcgggcccgc tgtgctgttc
     7441 atcctgacgc tgtatctcac cctggtcgcg caggcgttga tcctgccgta gcgaactcgt
     7501 gccggtgcgc ctcgtcgtgt cggccgccgt tccggtggaa caccagggac gatcggagat
     7561 tcgagcgata taacgcggaa gaaaggactg ctgtgaggac cctgctgtgg cgtatcggtg
     7621 gaggcggctg cctggtcacg ctcgcctccg ggctactgat cgcgggctcg gggtccgcgc
     7681 aggcagatcc cgacgcgtgc tacgtcgagc cggggatcac cgacgccgtc gcgcggtgcc
     7741 acgacggtgc gggtgcgtcc gcgctcgaag tcgaatgcct cgggttgatg acgcccagca
     7801 cgcccgacgg gccgatcttc ggcgcctact ccggcaccga atcggtgccg tcggcggtcg
     7861 gccagccgat gcgcgcgtcc tgcatctccg acgacaccct cggcatcacc acgcgggcgt
     7921 tcgtgacgcc gaggtaatcg gcctcaggac gtctcgtttc ccgtggcggc ctgctcgatc
     7981 ccggcacgca gcgtcagggc ggcccgctcg atccgtgcgg cctgtcctgt ccaattctct
     8041 tcggccgcac ccaggtacac ggctcgcgca tgagcgagca ccggtcggtg ctcctgcgcg
     8101 atccgatcca gcgcccagtc ggccgccttg tctttagcgg cgatcacacc ggtggcgacc
     8161 gtcatccaca cgcgggcgag agtgagaagc acattgcgcg tatcggttcc gagttcctcg
     8221 accagttccg gtacgcccgc caccatcgcg tgcgtcagat cggcccgggg gaccgggtcg
     8281 agcactcgcg cgggcggcgg ccccaccagc gtcgaatctc cgtccagcac catggttatc
     8341 agcaccgcca gatcaggact gactgtggcc gagggtattt ccccaccctc gtactccgcc
     8401 cgcaaccact ccccatactg aaaatcactc gtcggcggat accgccacgg ccgcaccgca
     8461 ccctgcacca ccgaggtcaa ttcgaccggc cgcagcgctc cccccacccc acccgacaac
     8521 tcgagcaacc cctccaacag cacccgccgc tgcgccccat ccaacgagtc cgcactcacc
     8581 accagcacat cgatatcgct gtaccgccgc aacccaccca acaccgccga cccatgccga
     8641 taaaccccga ccaactgctc cccgaacacc ctccgaacca gcccggcaac cccatcctcc
     8701 tgcgtcaccc caccacgcta accccactcc accaccgccc gaacgagccg gtcaggcggg
     8761 gagggtgtcg ccggggaagc tgaggcacgg gtggacttcg cagtgcatca tctgggcctg
     8821 gacgcagggg tctccgtcca tgatctcttt ggccttttcc ggtgtgacgg tcatgatggc
     8881 gatgcccgcc acggtgtcgg agacgacggg agtgaggatg gctatgacgc cgtcggcgcg
     8941 cagtgtcacc atgcgccgct ggtgttcgag ttcgattgcg gcggcgccgt ccatgtgtcg
     9001 ctgcgggccc cagtgcagga tggctacgct gtagggcttc gcggaggcgg ccagctgttg
     9061 gatctgctcg tctgtgatgg ttgtggtcat cggttcctcg atctcgtcgc gcgtttcccg
     9121 agactgtaac caggcgtaga ggtgtcggta ccagagatcg gatagcgggt cggcgaacaa
     9181 tacgagcact caagccactc tggtcgcggt gaccatgacg gtcgtgtagc ggcagacgaa
     9241 atcgcctccc gttcgatcga tgaccgcgcc gagttcggcg agtacttcgt ccagtacggc
     9301 cggtgccagg cgggtcgtgg cgccggtggt ggcggagtag tccaaccatt cgtcgcgggt
     9361 atagcgctgg gtccactcga aacgttgctg ttgcggctcg tcgaacgcgg ccgttccgcg
     9421 aatgccgtcg gcggctttgt cgcacatcgc ggtgtagatc tcgacgctgg acacgttggg
     9481 taggtgcgcg atgggggatt ccggaaccac gcggcggaac acctcggcga gggcctgttc
     9541 gagttcgggt ggcggctgtt gcacgttcca gaacagggcg acggtgccgc ctggacgtag
     9601 ggcggcggcg gccttgtgtg cgcccgcgac aggatcgacc cagtgccagg tctgcccggc
     9661 gacggcggcg tcgaaactgc gcccggctgg atcccaggtc tcgaaggtgg atacttcgac
     9721 ctcggcgccc cggtggcgtg cgaattcggc catgcgcgtg tcgggttcga cgccgaggac
     9781 ggtgcagccg gcggcctgga attgccgcgt gacgatgccg gtgccggctc cgatgtccag
     9841 gacgctgcgt cctggaatgg cgtcgatgac cgccgcgacg agttccgcgg gatagtgcgg
     9901 acggcatcgg tcgtagcgcg cggcatcgtt accgaatgat tccgcgatcg cgcgggctcg
     9961 atgggattcg agctgagggg cgggcgattg ccccggcggt atagtgggca tgtgcccact
    10021 gtaaatgggc aagtgcccac tcggcaaggg tggattcggc gacgaaaggg gatgtacacg
    10081 gtgccaacgg gagtcgccat gcgtgacgtg cgcgcgcagc tgttcgacgc cgccgaacgg
    10141 atcatgcgcc gggacgggcc gagcgcgctg accagtcggg cggtcaccac ggaggcgggg
    10201 tgcgcgaaag gcgtgctgca ccggcatttc gccgacttcg acgatttcct ggccgaactg
    10261 gtgctggatc gtgtcggcaa gctggaacag ctctcggccg cactgtatgt gtcggcgggc
    10321 accggctccg tggtcgaaaa cctctcggac gcactgacat cgatattcga gtcggtcgtg
    10381 gtggcgatcg tcggcctggt cacgttccgg gacgagttgc gcgccaggct acgtcaccgc
    10441 tggccgaccg ggttgccgct gatgtcggag gcgacggcga tgctggcgga gtacctcgcc
    10501 gccgaacgcg tgcacggccg gatcgcgaag aatgccgata tcgacgcgct cgcacccgct
    10561 ttgatcggca cggggcacct gctgttcgcc gaccgcacgg gaccgctgcc ggataccgcc
    10621 gccgtcatca gagtggtgac cactgtgatc ggtggtgcgt tgaccgccga gtagacgcct
    10681 ggcaggcata cgtggcgaac gagttcagct cggcacggct tcgagaatat tgtccggcga
    10741 caccgtcggg atcacccgtt gcaaggtgaa cccgtagcgc gccaagaact ctcgatactc
    10801 cgcggccgtg cgttcgcgcc cgccgacgtt caccagcatc tccagatcca cgaacttgcc
    10861 cgggtgcggg tgattgttct ccggcagcac cagctcgacg atcaccaggc gcgcaccggg
    10921 tttcatcgcc gcgcggactg cgctgaggat gcgtcccgcg tcctcttccg gc
//
