GCF_000220835.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_GL985606.1 (Actinomyces sp. oral taxon 448 str. F0400)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	lanthipeptide-class-ii	140,541	163,249
Region 1.2	azole-containing-RiPP	335,568	359,027
Region 1.3	azole-containing-RiPP	456,945	486,945	High	michiganin A	ribosomal:RiPP:Lanthipeptide
Region 1.4	terpene-precursor	2,042,473	2,063,630

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	lanthipeptide-class-ii	140,541	163,249
Region 1.2	azole-containing-RiPP	335,568	359,027
Region 1.3	azole-containing-RiPP	456,945	486,945	High	michiganin A	ribosomal:RiPP:Lanthipeptide
Region 1.4	terpene-precursor	2,042,473	2,063,630

No secondary metabolite regions were found in these records:: NZ_GL985607.1 (Actinomyces sp. oral taxon 448 str. F0400); NZ_GL985608.1 (Actinomyces sp. oral taxon 448 str. F0400); NZ_GL985609.1 (Actinomyces sp. oral taxon 448 str. F0400); NZ_GL985610.1 (Actinomyces sp. oral taxon 448 str. F0400); NZ_GL985611.1 (Actinomyces sp. oral taxon 448 str. F0400)

Compact view

NZ_GL985606.1 - Region 1 - lanthipeptide-class-ii

Download region GenBank file

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Location: 140,541 - 163,249 nt. (total: 22,709 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

HMPREF9062_RS00705: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.58	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000532.3	0.54	ribosomal	mutacin II	Streptococcus mutans
BGC0001210.5	0.52	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.51	ribosomal	thuricin	Bacillus thuringiensis
BGC0000539.5	0.51	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0001787.3	0.51	ribosomal	suicin 3908	Streptococcus suis
BGC0000547.5	0.49	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0000521.3	0.49	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0000527.5	0.49	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000498.5	0.48	ribosomal	bovicin HJ50	Streptococcus equinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.58	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000532.3	0.54	ribosomal	mutacin II	Streptococcus mutans
BGC0001210.5	0.52	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.51	ribosomal	thuricin	Bacillus thuringiensis
BGC0000539.5	0.51	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0001787.3	0.51	ribosomal	suicin 3908	Streptococcus suis
BGC0000547.5	0.49	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0000521.3	0.49	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0000527.5	0.49	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000498.5	0.48	ribosomal	bovicin HJ50	Streptococcus equinus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for HMPREF9062_RS00705

HMPREF9062_RS00700

Leader:

MTADILVEMTDAELDATLGA

Core:

GNGVFHTISHECYYNSWAFIFTCC

Core with dehydrations:

GNGVFHDhbIDhaHECysYYNDhaWAFIFDhbCysCys

antiSMASH-DB 4.0 matches for HMPREF9062_RS00700

100.0%

NZ_GL985606.1:

Lanthipeptides: HMPREF9062_RS00700_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

75.0%

NZ_QTVL01000002.1and 5 others:

NZ_QTVL01000002.1 Lanthipeptides: DWZ44_RS04150_lanthipeptideNZ_QTZB01000015.1 Lanthipeptides: DXC15_RS14340_lanthipeptideNZ_QTZB01000015.1 Lanthipeptides: DXC15_RS14335_lanthipeptideNZ_QUKU01000003.1 Lanthipeptides: DXC27_RS02940_lanthipeptideNZ_QUKU01000003.1 Lanthipeptides: DXC27_RS02935_lanthipeptide

Lanthipeptides: DWZ44_RS04155_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

G+GVF TISHEC N+W FIFTCC

GDGVFRTISHECNMNTWQFIFTCC

72.0%

NZ_CP018188.1and 17 others:

NZ_CP018188.1 Lanthipeptides: BSR19_RS11175_lanthipeptideNZ_CP066093.1 Lanthipeptides: I6I02_RS07300_lanthipeptideNZ_CP066093.1 Lanthipeptides: I6I02_RS07295_lanthipeptideNZ_JALDUD010000010.1 Lanthipeptides: M1G10_RS09705_lanthipeptideNZ_JALDUD010000010.1 Lanthipeptides: M1G10_RS09700_lanthipeptideNZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09300_lanthipeptideNZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09295_lanthipeptideNZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09300_lanthipeptideNZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09295_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06585_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06580_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06585_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06580_lanthipeptideNZ_KV832031.1 Lanthipeptides: HMPREF2637_RS05120_lanthipeptideNZ_KV832031.1 Lanthipeptides: HMPREF2637_RS05115_lanthipeptideNZ_PKIA01000004.1 Lanthipeptides: CYK22_RS04335_lanthipeptideNZ_PKIA01000004.1 Lanthipeptides: CYK22_RS04330_lanthipeptide

Lanthipeptides: BSR19_RS11180_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

GNGVF TISHEC+ N+WAF+ TCC

GNGVFKTISHECHLNTWAFLATCC

with 61 more sequence matches

Only the first 10 are displayed here.

72.0%

NZ_CP026729.1:

Lanthipeptides: BKKJ1_RS07010_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

GNGV TISHEC NSW F+FTCC

GNGVVTTISHECNMNSWQFLFTCC

72.0%

NZ_FOZB01000002.1:

Lanthipeptides: BM306_RS07850_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

G+GVF TISHEC+ N+W FIFTCC

GDGVFRTISHECHMNTWMFIFTCC

72.0%

NZ_GL882519.1:

Lanthipeptides: HMPREF9056_RS03330_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

GNGV++T+SHEC+ NSW FIFTCC

GNGVYYTLSHECHMNSWQFIFTCC

72.0%

NZ_JAEEGA010000010.1:

Lanthipeptides: I6N95_RS15660_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

GNGV TISHEC+ NSW +IFTCC

GNGVIKTISHECHMNSWQWIFTCC

72.0%

NZ_LT732526.1and 1 other:

NZ_LT732526.1 Lanthipeptides: BQ9856_RS23075_lanthipeptide

Lanthipeptides: BQ9856_RS23080_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

GNGV TISHEC N+W FIFTCC

GNGVIKTISHECAMNTWQFIFTCC

70.8%

NZ_AP025017.1:

Lanthipeptides: MANAM107_RS05920_lanthipeptide

NGVFHTISHECYYNSWAFIFTCC

NGV TISHEC+ N+WAF FTCC

NGVITTISHECHLNTWAFAFTCC

70.8%

NZ_CP084014.1and 6 others:

NZ_CP084014.1 Lanthipeptides: LCQ72_RS00100_lanthipeptideNZ_CP084014.1 Lanthipeptides: LCQ72_RS00095_lanthipeptideNZ_CP094679.1 Lanthipeptides: K5I24_RS13105_lanthipeptideNZ_JAQEYW010000015.1 Lanthipeptides: PG422_RS12415_lanthipeptideNZ_JAQEYW010000015.1 Lanthipeptides: PG422_RS12410_lanthipeptideNZ_JAQEYW010000015.1 Lanthipeptides: PG422_RS12405_lanthipeptide

Lanthipeptides: LCQ72_RS00105_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

GNGV TISHEC N+W F+FTCC

GNGVLKTISHECNMNTWQFLFTCC

70.8%

NZ_MSRJ01000005.1:

Lanthipeptides: BKH06_RS03755_lanthipeptide

GVFHTISHECYYNSWAFIFTCC

GV TISHEC NSW FIFTCC

GVVTTISHECNMNSWQFIFTCC

69.2%

NC_012470.1and 11 others:

NZ_CABY01000005.1 Lanthipeptides: SZBHS5_RS06485_lanthipeptideNZ_CP029398.1 Lanthipeptides: DF184_RS05185_lanthipeptideNZ_CP029398.1 Lanthipeptides: DF184_RS05180_lanthipeptideNZ_CP078012.1 Lanthipeptides: KV238_RS09835_lanthipeptideNZ_CP078012.1 Lanthipeptides: KV238_RS09830_lanthipeptideNZ_JAFLMI010000009.1 Lanthipeptides: J0638_RS05350_lanthipeptideNZ_JAFLMI010000009.1 Lanthipeptides: J0638_RS05345_lanthipeptideNZ_LR738724.1 Lanthipeptides: GPW69_RS05065_lanthipeptideNZ_LR738724.1 Lanthipeptides: GPW69_RS05060_lanthipeptideNZ_VZNS01000007.1 Lanthipeptides: F6P79_RS05580_lanthipeptideNZ_VZNS01000007.1 Lanthipeptides: F6P79_RS05575_lanthipeptide

Lanthipeptides: SZO_RS04070_lanthipeptide

NGVFHTISHECYYNSWAFIFTCC

NGVF TISHEC+ NSW F+FTCC

NGVFKTISHECHMNSWQFLFTCC

68.0%

NZ_JH792236.1and 1 other:

NZ_JH792236.1 Lanthipeptides: IIM_RS26240_lanthipeptide

Lanthipeptides: IIM_RS26245_lanthipeptide

GNGVFHTISHECYYNSWAFIFTCC

G+GV TISHEC+ NSW IFTCC

GSGVIKTISHECHMNSWQAIFTCC

MIBiG 4.0 matches for HMPREF9062_RS00700

20.0%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

NGVFHTISHECYYN

NG + T++ EC N

NGGWCTLTKECQPN

12.8%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

TISHECYYN

T+S EC N

TLSKECQPN

allorf_0144793_0144999

Leader:

MPTPTIDARASGRDSGRGMKRPARRVRAGG

Core:

REAVRSRPIEQTVVYCNETMPFPSVAAGRCRVWHTRTM

Core with dehydrations:

REAVRDhaRPIEQDhbVVYCysNEDhbMPFPDhaVAAGRCysRVWHDhbRDhbM

antiSMASH-DB 4.0 matches for allorf_0144793_0144999

100.0%

NZ_GL985606.1:

Lanthipeptides: allorf_0144793_0144999_lanthipeptide

REAVRSRPIEQTVVYCNETMPFPSVAAGRCRVWHTRTM

18.4%

NZ_CP006910.1:

Lanthipeptides: W903_RS05325_lanthipeptide

AAGRCRVWHTRT

AAGRC W T +

AAGRCGRWFTGS

17.1%

NZ_KB235922.1:

Lanthipeptides: PLEUR7319_RS41230_lanthipeptide

IEQTVVYCNETMPFPSVAAGR

I + C T+P PS+ R

IANLSLQCGVTLPIPSIWGHR

with 1 more sequence matches

13.2%

NZ_LT800494.1:

Lanthipeptides: allorf_0519127_0519222_lanthipeptide

IEQTVVYCNETMPFPS

I + +++C MP S

IPEFIIFCKNVMPLKS

allorf_0150104_0150241

Leader:

MPCGTGALVAEVVCHPGVTVVAELLSGA

Core:

VCVTEQYLCRTFCFTVI

Core with dehydrations:

VCysVDhbEQYLCysRDhbFCysFDhbVI

antiSMASH-DB 4.0 matches for allorf_0150104_0150241

100.0%

NZ_GL985606.1:

Lanthipeptides: allorf_0150104_0150241_lanthipeptide

VCVTEQYLCRTFCFTVI

14.0%

NZ_JACEIQ010000001.1and 1 other:

NZ_JACEIQ010000001.1 Lanthipeptides: H1191_RS00055_lanthipeptide

Lanthipeptides: H1191_RS00055_lanthipeptide

TEQYLCRTFC

TEQ L R +C

TEQELLRAYC

9.1%

NZ_EQ973330.1:

Lanthipeptides: allorf_437242_437442_lanthipeptide

LCRTFCFTV

LCR CF++

LCRGACFSI

MIBiG 4.0 matches for allorf_0150104_0150241

22.7%

BGC0000563.4 (AEA03263.1):

Lanthipeptide: venezuelin

CVTEQYLCRTFCFTV

CV L T C +

CVGLLTLLNTVCIGI

14.7%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

VTEQYLCRTFCFT

+T LC C T

ITSISLCTPGCKT

allorf_0159575_0159772

Leader:

MPFRDDSLCHG

Core:

NLGNWTALRPVATRTEAHEPLVPCLAESVIATTNRSKAARRIRPGSPPTASRSP

Core with dehydrations:

NLGNWDhbALRPVADhbRDhbEAHEPLVPCysLAEDhaVIADhbDhbNRDhaKAARRIRPGDhaPPDhbADhaRDhaP

antiSMASH-DB 4.0 matches for allorf_0159575_0159772

100.0%

NZ_GL985606.1:

Lanthipeptides: allorf_0159575_0159772_lanthipeptide

NLGNWTALRPVATRTEAHEPLVPCLAESVIATTNRSKAARRIRPGSPPTASRSP

16.7%

NZ_CP039292.1and 1 other:

NZ_CP039292.1 Lanthipeptides: allorf_0079649_0079816_lanthipeptide

Lanthipeptides: allorf_0079649_0079816_lanthipeptide

IATTNRSKAARRIRPGSPPTASR

+ +R K RR PG P+A R

VVVADRDKCVRR--PGCFPSAER

allorf_0159778_0159840

Leader:

MDVRVRRSQSIGS

Core:

ASGCSVL

Core with dehydrations:

ADhaGCysDhaVL

antiSMASH-DB 4.0 matches for allorf_0159778_0159840

100.0%

NZ_GL985606.1:

Lanthipeptides: allorf_0159778_0159840_lanthipeptide

ASGCSVL

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (5):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.54 out of 30.22

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.15 out of 25.75

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.88 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.08 out of 21.55

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

HMPREF9062_RS00700 - Class II

Cleavage pHMM score: 1.50

RODEO score: 0

Monoisotopic mass: 2726.2 Da

Molecular weight: 2728.0 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2746.0 Da N = 1

allorf_0144793_0144999 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 4325.2 Da

Molecular weight: 4328.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4346.0 Da N = 1; 4364.0 Da N = 2; 4382.0 Da N = 3; 4400.1 Da N = 4

allorf_0150104_0150241 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 1970.0 Da

Molecular weight: 1971.4 Da

Number of bridges: 3

allorf_0159575_0159772 - Class II

Cleavage pHMM score: -7.10

RODEO score: 0

Monoisotopic mass: 5575.1 Da

Molecular weight: 5578.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5596.3 Da N = 1; 5614.3 Da N = 2; 5632.4 Da N = 3; 5650.4 Da N = 4; 5668.4 Da N = 5; 5686.4 Da N = 6; 5704.4 Da N = 7; 5722.5 Da N = 8; 5740.5 Da N = 9; 5758.5 Da N = 10

allorf_0159778_0159840 - Class II

Cleavage pHMM score: -2.50

RODEO score: 0

Monoisotopic mass: 599.3 Da

Molecular weight: 599.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 617.7 Da N = 1

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein

NZ_GL985606.1 - Region 2 - azole-containing-RiPP

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Location: 335,568 - 359,027 nt. (total: 23,460 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

HMPREF9062_RS01530: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

HMPREF9062_RS01565: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002584.2	0.44	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000569.5	0.41	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002635.2	0.41	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0001173.5	0.41	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000565.5	0.41	ribosomal	goadsporin	Streptomyces sp. TP-A0584
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000483.5	0.40	ribosomal	aeruginosamide B, aeruginosamide C	Microcystis aeruginosa PCC 9432

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002584.2	0.44	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000569.5	0.41	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002635.2	0.41	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0001173.5	0.41	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000565.5	0.41	ribosomal	goadsporin	Streptomyces sp. TP-A0584
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000483.5	0.40	ribosomal	aeruginosamide B, aeruginosamide C	Microcystis aeruginosa PCC 9432

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.27 out of 24.78

Confidence: medium

Additional weak hits (2):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.11 out of 32.74

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.60 out of 28.19

Confidence: weak

(Thi)azol(in)e containing peptide predictions

(Thi)azol(in)e containing peptide(s)

HMPREF9062_RS01570

Leader:

MVDHESQGDVQFDEKALARVMGRAYADDAYREVLTSSPSETLEQAGALASGSIATAALDAELEDWDYAVHHDENGAHLRIGLPDLEEELDTSV

Core:

PEDGLTVACCCCCPC

Core with dehydrations:

PEDGLDhbVACysCysCysCysCysPCys

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
LexA variant 2	Repressor of DNA damage response
MexT	Global virulence regulator

Core peptide predictions

HMPREF9062_RS01570

Putative Type III

Cleavage pHMM score: -2.90

RODEO score: 20

Monoisotopic mass: 1515.5 Da

Molecular weight: 1516.8 Da

Predicted peptide features:
Central ring: pyridine trisubstituted

Alternative weights: (assuming N unmodified Ser/Thr residues); 1534.8 Da N = 1

NZ_GL985606.1 - Region 3 - azole-containing-RiPP

Download region GenBank file

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Location: 456,945 - 486,945 nt. (total: 30,001 nt) Show pHMM detection rules used

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

HMPREF9062_RS02145: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: lyase

HMPREF9062_RS02135: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.58	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002311.3	0.48	ribosomal	cutimycin	Cutibacterium acnes KPA171202
BGC0002584.2	0.45	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0002316.2	0.45	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001472.5	0.45	ribosomal	berninamycin A	Streptomyces bernensis
BGC0002346.3	0.42	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002363.3	0.42	ribosomal	berninamycin K, berninamycin J, berninamycin A, berninamycin B	Streptomyces sp.
BGC0000556.5	0.41	ribosomal	streptin	Streptococcus pyogenes
BGC0000569.5	0.41	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001174.4	0.41	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.58	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002311.3	0.48	ribosomal	cutimycin	Cutibacterium acnes KPA171202
BGC0002584.2	0.45	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0002316.2	0.45	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001472.5	0.45	ribosomal	berninamycin A	Streptomyces bernensis
BGC0002346.3	0.42	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002363.3	0.42	ribosomal	berninamycin K, berninamycin J, berninamycin A, berninamycin B	Streptomyces sp.
BGC0000556.5	0.41	ribosomal	streptin	Streptococcus pyogenes
BGC0000569.5	0.41	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001174.4	0.41	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 30.10 out of 32.38

Confidence: strong

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 26.07 out of 30.12

Confidence: medium

Additional weak hits (4):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.82 out of 20.01

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.26 out of 35.63

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 25.01 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.75 out of 28.19

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
CsoR	Copper-responsive repressor
OsdR	Development and stress management regulator

Core peptide predictions

No core peptides found.

NZ_GL985606.1 - Region 4 - terpene-precursor

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Location: 2,042,473 - 2,063,630 nt. (total: 21,158 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase phosphorylase

HMPREF9062_RS08710: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

HMPREF9062_RS08745: acyltransferase

smcogs: SMCOG1141: acyltransferase

Ligases: synthetase

HMPREF9062_RS08725: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0000936.5	0.37	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001456.3	0.36	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.36	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000664.5	0.36	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000643.3	0.35	terpene	carotenoid	Brevundimonas vesicularis
BGC0000633.3	0.35	terpene	carotenoid	Streptomyces avermitilis
BGC0000644.3	0.35	terpene	carotenoid	Dietzia sp. CQ4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0000936.5	0.37	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001456.3	0.36	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.36	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000664.5	0.36	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000643.3	0.35	terpene	carotenoid	Brevundimonas vesicularis
BGC0000633.3	0.35	terpene	carotenoid	Streptomyces avermitilis
BGC0000644.3	0.35	terpene	carotenoid	Dietzia sp. CQ4

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

HMPREF9062_RS08725: PT_FPPS_like

Domain 1 (49 - 363): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 24.07 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.38 out of 24.78

Confidence: medium

Additional weak hits (9):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.29 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.25 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.26 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.16 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.65 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.87 out of 33.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.99 out of 23.43

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.44 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response