LOCUS       NZ_CP023202            10405 bp    DNA     linear   CON 21-MAR-2024
DEFINITION  Streptomyces xinghaiensis S187 chromosome, complete genome.
ACCESSION   NZ_CP023202
VERSION     NZ_CP023202.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces xinghaiensis S187
  ORGANISM  Streptomyces xinghaiensis S187
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7137891)
  AUTHORS   Zhao,X. and Yang,T.
  TITLE     Draft genome sequence of the marine sediment-derived actinomycete
            Streptomyces xinghaiensis NRRL B24674T
  JOURNAL   J Bacteriol 193 (19), 5543 (2011)
   PUBMED   21914869
REFERENCE   2  (bases 1 to 7137891)
  AUTHORS   Chen,L., Zhao,X. and Lin,S.
  TITLE     Genome mining of a marine derived strain Streptomyces xinghaiensis
            reveals biosynthetic ability of xiamycin analogues
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7137891)
  AUTHORS   Chen,L., Zhao,X. and Lin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2017) School of Life Science and Biotechnology,
            Dalian University of Technology, Linggong Road 2nd, Dalian, Liaoning
            116023, China
REFERENCE   4  (bases 1 to 7137891)
  AUTHORS   Zhao,X., Yang,T. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2011) School of Life Science and Biotechnology,
            Dalian University of Technology, Linggong Road 2, Dalian, Liaoning
            116024, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2017
            Assembly Method                   :: Canu v. 1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: Streptomyces xinghaiensis S187 genome
            sequencing
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000220705.2-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 05:53:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,157
            CDSs (total)                      :: 6,072
            Genes (coding)                    :: 5,922
            CDSs (with protein)               :: 5,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 36 of 150
            Pseudo Genes (incomplete)         :: 134 of 150
            Pseudo Genes (internal stop)      :: 6 of 150
            Pseudo Genes (multiple problems)  :: 26 of 150
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:47:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5876342
            Orig. end                         :: 5886747
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023202.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Shanghai Jiaotong
            University, School of Life Science and Biotechnology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    241..256
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.17"
                     /tool="antismash"
     gene            316..1848
                     /locus_tag="SXIN_RS25060"
     CDS             316..1848
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477745.1"
                     /locus_tag="SXIN_RS25060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_039821870.1"
                     /sec_met_domain="Aldedh (E-value: 2.4e-41, bitscore: 140.2,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATAVWSVDPRTGKQREEVAVEATPAEVDEAVRAAHAVRGSLADR
                     AVRTAFLRTAADLLDEAGDHVVEAADAETALGPGRLTGELARTTYQLRAFADLVDEGSF
                     LGVMIDHADPDLKPVPRPDLRRYKVPLGVVAVYSASNFPLAFSVPGGDTASALAAGCPV
                     VVKAHPDHPATSELCASVLRRAAVQAGLPADVVRLVHGFEAGIELIRHPLVSAAGFTGS
                     VRGGRALFDAAAARPVPIPFHGELGSLNPVVVTEAAADERAEEIGTGLAGSMTLGTGQF
                     CVKPGLVLAPSGDAGDRLLSALTDAVSRTEAGVLLDTRMRDNFVRGVRERLDLPGVASP
                     VTPGESGELAVGAGYLTVPAARLAGGDAHELLLEECFGPVTVVVRYENAAEVSAVLERL
                     PGNLTATVHIGEREAAEENGPGARLLAELTPLAGRLLVNGWPTGVAVAPAQHHGGPYPA
                     TTSTSTSVGSTAIERWLRPVAYQNTPQALLPPELRDGNPLGLPRRVDGRPEL"
     gene            2044..2703
                     /locus_tag="SXIN_RS25065"
     CDS             2044..2703
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /locus_tag="SXIN_RS25065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_095757569.1"
                     /transl_table=11
                     /translation="MPEGNAAVHEAYDAVRTRMTDGRYAQGQILTPEAVATETSLPLPA
                     IKAAFGRLAEENALRFHPPQGALILPLDLEEARDVMEARLLLEMFAIDSVAARGREHAR
                     RLGRALEPLLHEKDTSEATLLQARQFHGELVGAAGNAVVALMHETLWDRSLHVAAASTA
                     GPDNRRGNVAEHRAIAEALCRGEGARARRLVYEHIAAILRRVGVGDELALPRPADA"
     gene            2810..3874
                     /locus_tag="SXIN_RS25070"
     CDS             2810..3874
                     /NRPS_PKS="Domain: Aminotran_5 (118-343). E-value: 7.8e-24.
                     Score: 76.2. Matches aSDomain:
                     nrpspksdomains_SXIN_RS25070_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180697.1"
                     /locus_tag="SXIN_RS25070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_019709611.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.9e-17, bitscore:
                     60.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTLGGAHFAPKSTYLNTASMGLLPREAAAVLREGVDGTADGRP
                     SPDLGFPSVDRARAAFARLAGVDPGRVATSGAVAVHTGLIAGSLPEGAEVLIADGDFSS
                     LVNPFAVRPDLKLRSVPLTALADEVRPGTALVAVSAVQSADGRIADLAAIRAAARAHGA
                     RTLVDATQALGWFPVDAGDFDYTVTGAYKWLLCPRGVSFLTVPEDGGGLRPVFSGWTAG
                     EDPWESCYGPVRRLARTARRFDESHALLAYLAAPEALAVIEETGPEAIRAHNLALAERC
                     RAGLAGLGYEPVSEPGSAIVAVPGLGHAEETLAAADVRVAIRAGNLRAAFHLYNTAADV
                     DRLLDVLSGLPARP"
     aSDomain        3164..3838
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SXIN_RS25070_Aminotran_5.1"
                     /evalue="7.80E-24"
                     /label="SXIN_RS25070_Aminotran_5.1"
                     /locus_tag="SXIN_RS25070"
                     /protein_end="343"
                     /protein_start="118"
                     /score="76.2"
                     /tool="antismash"
                     /translation="SVPLTALADEVRPGTALVAVSAVQSADGRIADLAAIRAAARAHGA
                     RTLVDATQALGWFPVDAGDFDYTVTGAYKWLLCPRGVSFLTVPEDGGGLRPVFSGWTAG
                     EDPWESCYGPVRRLARTARRFDESHALLAYLAAPEALAVIEETGPEAIRAHNLALAERC
                     RAGLAGLGYEPVSEPGSAIVAVPGLGHAEETLAAADVRVAIRAGNLRAAFHLYNTAADV
                     DRL"
     gene            complement(4037..4936)
                     /gene="thpD"
                     /locus_tag="SXIN_RS25075"
     CDS             complement(4037..4936)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848585.1"
                     /locus_tag="SXIN_RS25075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_019709612.1"
                     /sec_met_domain="PhyH (E-value: 4.9e-39, bitscore: 133.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIAPERTTDLYPTRGATEVATPRVDPVVWSAPGTPGPISENEIS
                     DFDRDGFMAVDQLITPDEVAVYRDELNRLVTDPEVKADERSIVEPKSQKVRSVFEVHKI
                     SEVFAGLVRDPRVLDRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHKTFLGCAGETPKDNYKRSLQMQDAGIPSDEA
                     LSGFAEKHGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENPAVEPFA
                     APIRRPEFLGARDFTPVK"
     misc_feature    complement(4993..5007)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            complement(5001..5405)
                     /locus_tag="SXIN_RS25080"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="SXIN_RS25080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_019709613.1"
                     /sec_met_domain="ectoine_synt (E-value: 6e-54, bitscore:
                     179.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLRDIEGTDRHIKSQSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETKMWYANHVEAVLCVEGEAELTNEETGEKHWIEPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPEEG"
     gene            complement(5492..6760)
                     /gene="ectB"
                     /locus_tag="SXIN_RS25085"
     CDS             complement(5492..6760)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 6.2e-75.
                     Score: 244.2. Matches aSDomain:
                     nrpspksdomains_SXIN_RS25085_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848583.1"
                     /locus_tag="SXIN_RS25085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_019709614.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.6e-78, bitscore:
                     261.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFESLESEVRSYCRGWPAVFDRAKGSYMYDEDGHTY
                     LDFFSGAGALNYGHNNEVLKRALLDYLERDGVTHGLDMGTTAKRAFLESFQNSILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYFDGKVPDFLWFERLLEDQGSGLNQPAAVIVETVQGEGGINVARA
                     DWLRALADLCRRRDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSISGYGMPMS
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTISRGEQVEQALT
                     AITEEHPRLGGVVRGRGLVWGMEFTDKSRASAVCKRAFELGLLVETSGPQSEVVKLLPA
                     LTTTPEELDEGLRILARAVRETV"
     aSDomain        complement(5666..6676)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SXIN_RS25085_Aminotran_3.1"
                     /evalue="6.20E-75"
                     /label="SXIN_RS25085_Aminotran_3.1"
                     /locus_tag="SXIN_RS25085"
                     /protein_end="365"
                     /protein_start="28"
                     /score="244.2"
                     /tool="antismash"
                     /translation="FDRAKGSYMYDEDGHTYLDFFSGAGALNYGHNNEVLKRALLDYLE
                     RDGVTHGLDMGTTAKRAFLESFQNSILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNSMKRAGAGIPLVHGTPMPFDNYFDGKVPDFLWFER
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARADWLRALADLCRRRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSISGYGMPMSLTLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTISRGEQVEQALTAITEEHPRLGGVVRGRGLVWGMEFT"
     gene            complement(6861..7424)
                     /gene="ectA"
                     /locus_tag="SXIN_RS25090"
     CDS             complement(6861..7424)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577188.1"
                     /locus_tag="SXIN_RS25090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_176743615.1"
                     /transl_table=11
                     /translation="MTAAQADHAGPRTEFLEMPEGLKLDTPRVDDGAAIWRIARDSEVL
                     DLNSSYSYLLWCRDFAATSVVARGADGEPVGFVTGYLRPEAPRTLVVWQVAVDESQRGR
                     GLAGALLDGLTERVAHEKDIDRVETTITPDNTASNRLFTSFAERHGAAVEREVLFDPGL
                     FPEEGHLPEVLYRIGPLPWTAPPR"
     misc_feature    complement(7433..7447)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.34"
                     /tool="antismash"
     misc_feature    complement(7582..7597)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7592..7607
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7592..7609)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.82"
                     /tool="antismash"
     misc_feature    7681..7695
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.13"
                     /tool="antismash"
     misc_feature    7701..7715
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    7752..7764
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    7759..7774
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.49"
                     /tool="antismash"
     gene            7872..8963
                     /locus_tag="SXIN_RS25095"
     CDS             7872..8963
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="SXIN_RS25095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_095757570.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 6.4e-19, bitscore:
                     66.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGTVLHIKGRVLRGPEDVRDELWVRDGRVTYEPPAAARKVTTL
                     TGWVLPGLVDAHCHVGLDARGPVDESTGEKQALTDRDAGTLLIRDAGSPSDTRWIDDRE
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRSTGD
                     LAPCWPRGEVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPRLADGGQEKFPRWSDHMRRLHERRYETVAAAHDAGIPVFAG
                     TDAGGSLPHGLIAQEVAELVKAGLPAATALSAATWGAREWLGRPGLSEGAPADLVVYDT
                     DPREDVRVLAAPRHVVLRGRVTG"
     gene            9187..10242
                     /locus_tag="SXIN_RS25100"
     CDS             9187..10242
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828649.1"
                     /locus_tag="SXIN_RS25100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_192883631.1"
                     /transl_table=11
                     /translation="MNSNAFVPPVRRTAAALTAAALAAAPVLLAGAAPAYASGGDGKAG
                     AVVLRADLGVTLLDQTRVPLNVTLNEVQAPESASETLLTARVEGAHRGRPVEVLKAAVA
                     TAEAKTEAGKAEGRANLVNARVFVPGLKKHPLIELDQVTSTAVCPAGGQPTAEAKIPGH
                     VTVFGQRVELSAEGTARADVEGIGSVELALAKTTTTATTAAATALELQVSVTPELTAPK
                     KLNVAAVEGKVTLVEATCESPAGGSGDGGPGTGSGGATEGATGGSGGSGDGSGDGGSGD
                     GSGDGSGDGSGDGSGDGVSTQTGSGSGEDNLAATGGNSTTPYLAAGAGALLAAGGVSVF
                     VARRRAAARRG"
ORIGIN
        1 ccgtacctgg tggcccggca cgcgggcgcc ggccggcgct gagccgggcg cacggtcccg
       61 gccccgggcg gggcgccggg gccgtccggg ccggccggtc cctccggtgc ttccgggagt
      121 ccggccggcc cgggcacgcc gcacgcccgc gcccgcgggt agtacgggcc cgggcccggg
      181 cgctccgcgg agcaccgggc gcccagcctt gaccctctcc gcgccccgga aatagggtcc
      241 aagggtattc agcatcagga acagcgttca gtatctcgaa cgccgcctac gtggtatgag
      301 ctggaggaac cgcaagtggc cacagcagtg tggagcgtcg atccccggac cgggaagcag
      361 cgcgaggaag tggccgtcga ggccacgccc gcggaggtcg acgaggccgt gcgcgccgcg
      421 cacgcggtcc gcggcagcct cgccgaccgc gccgtgcgca ccgccttcct gcgcacggcc
      481 gccgacctcc tggacgaggc cggtgaccat gtcgtcgagg ccgccgacgc ggagaccgcg
      541 ctcggccccg gccggctcac cggcgagctg gcccggacga cgtaccagct cagggcgttc
      601 gccgacctcg tcgacgaggg ctccttcctc ggcgtgatga tcgaccacgc cgacccggac
      661 ctgaagcccg tcccgcgccc cgacctgcgc cgctacaagg tcccgctcgg cgtcgtcgcc
      721 gtctactccg cgagcaactt cccgctcgcc ttctccgtcc ccggcgggga cacggccagc
      781 gcgctcgccg ccggctgccc cgtcgtcgtc aaggcgcacc ccgaccaccc ggcgacctcc
      841 gaactctgcg cctccgtgct gcgccgcgcc gccgtacagg cggggctccc ggccgatgtc
      901 gtccgcctgg tgcacggctt cgaggcgggc atcgagctga tcaggcaccc gctggtctcg
      961 gccgccgggt tcaccggctc ggtccgcggc ggccgggcgc tcttcgacgc ggccgccgcc
     1021 cgccccgtcc ccatcccgtt ccacggcgag ctgggcagcc tcaacccggt cgtcgtcacc
     1081 gaggccgcgg cggacgagcg cgccgaggag atcggcaccg gcctcgccgg gtccatgacc
     1141 ctcggcaccg ggcagttctg cgtcaagccg ggcctggtgc tggccccctc cggcgacgcc
     1201 ggggaccggc tgctgtccgc cctcaccgac gcggtcagca ggaccgaggc gggggtgctg
     1261 ctcgacaccc ggatgcgcga caacttcgtc cgcggggtgc gcgagcggct ggacctcccc
     1321 ggggtcgcca gcccggtcac ccccggcgag agcggcgagc tggccgtggg cgccggatac
     1381 ctcaccgtcc cggcggcacg gctcgccggg ggcgacgccc acgagctgct gctggaggag
     1441 tgcttcggcc cggtcaccgt cgtcgtccgc tacgagaacg ctgccgaggt ctccgcggtc
     1501 ctggagcggc tgcccggcaa cctcaccgcc accgtccaca tcggcgagcg ggaggcggcc
     1561 gaggagaacg gcccgggcgc gcggctgctc gccgagctga ccccgctggc cggccggctg
     1621 ctggtgaacg gctggcccac gggcgtcgcc gtggccccgg cacagcacca cggcggtccc
     1681 tacccggcca ccacctccac ctccacctcg gtgggctcca ccgcgatcga gcgctggctc
     1741 cgcccggtgg cgtaccagaa caccccgcag gcgctgctgc cccccgagct cagggacggc
     1801 aacccgctgg gcctcccgcg ccgggtcgac ggccggccgg agctctgacc ggaaccacgt
     1861 cccccggtgc ccgcgggcac cgggggacct gttcggcgta acggaccggc gccggaggga
     1921 gacgcccgcc cgcggccgga gagcggccgg agagcggccg gagagagggg cgcggccgcg
     1981 ccccgcgccg cctccccgcc ggacacggag tgccgcccgc cgcggaaacg ggacaatggg
     2041 ggcatgccgg aagggaatgc cgcggtgcac gaggcgtacg acgccgtccg gacccgtatg
     2101 acggacggcc gctacgccca ggggcagatc ctcaccccgg aagcggtcgc cacggagacg
     2161 agtctcccgc tcccggcgat caaggcggcc ttcgggcgtc tggccgagga gaacgccctg
     2221 cggttccatc cgccccaggg cgcgctgatc ctgccgctgg acctggagga ggcccgcgac
     2281 gtcatggagg cccggctgct cctggagatg ttcgcgatcg actcggtggc cgcccgcggc
     2341 cgggagcacg cacgccggct gggccgggcc ctcgaaccgc tgctgcacga gaaggacacc
     2401 tccgaggcga ccctgctcca ggcccggcag ttccacgggg agctggtcgg ggcggcgggc
     2461 aacgccgtgg tcgccctgat gcacgagacg ctctgggacc gcagcctgca cgtggccgcc
     2521 gcctccaccg ccgggccgga caaccggcgc ggcaacgtcg cggagcaccg ggccatcgcc
     2581 gaggcgctgt gccgcggcga gggcgcccgg gcccgccggc tggtgtacga gcacatcgcc
     2641 gccatcctgc gccgcgtcgg cgtcggcgac gagctggccc tgccgcgccc ggcggacgcc
     2701 tgacgcctga cgcccgacgc ccgacgcacc cccgcgcccc gtcgggcatc cgccgcgcgg
     2761 ccacaacccc gtggacggga ggacgccgtg ccgcgcatag tggctggcta tggagaccac
     2821 tctgggaggg gcgcacttcg cgccgaagtc gacctatctc aacaccgctt cgatggggct
     2881 gctgccccgg gaggccgcgg ccgtgctccg ggagggggtg gacggcaccg ccgacggccg
     2941 tccctcgccc gacctcggct tcccctcggt ggaccgggcc cgtgcggcct tcgcccggct
     3001 ggccggagtg gaccccgggc gcgtggccac ctcgggcgcc gtcgccgtcc acaccggcct
     3061 catcgccggc tcactgccgg aaggggccga agtcctcatc gccgacgggg acttcagctc
     3121 gctggtcaac cccttcgccg tccgtcccga tctcaagctg cgcagtgtgc cgctcaccgc
     3181 cctggcggac gaggtccggc ccggtacggc gctcgtcgcc gtcagcgccg tccagtccgc
     3241 cgacggccgg atcgccgacc tcgccgcgat ccgtgcggcc gcccgcgccc acggcgcccg
     3301 tacgctcgtc gacgccaccc aggccctcgg ctggttcccg gtggacgccg gcgacttcga
     3361 ctacaccgtc accggcgcct acaagtggct gctctgcccg cgcggtgtct ccttcctcac
     3421 cgtcccggag gacggcgggg gcctgcgccc ggtcttctcc ggctggacgg cgggggagga
     3481 cccctgggag agctgttacg gcccggtgcg ccggctggcc cgcaccgcac gccggttcga
     3541 cgagagccac gcgctgctgg cgtatctcgc ggccccggag gcgctggcgg tcatcgagga
     3601 gaccggcccc gaagccatcc gcgcccacaa cctcgcgctc gccgagcgct gccgggccgg
     3661 gctggccggg ctgggctacg agccggtgtc cgagccgggt tccgcgatcg tcgccgtccc
     3721 cgggctgggc cacgccgagg agacgctggc tgcggcggac gtccgcgtgg ccatccgggc
     3781 cggcaatctg cgggcggcct tccacctcta caacaccgcc gccgacgtgg accggctgct
     3841 cgacgtcctc tcgggcctcc cggcccgccc ctgacccggc cccgggtacg cgacggcggc
     3901 cgtcaccggg aatgcctccc ggcgacggcc gccgtcactc gcccagtggg agcccgatcc
     3961 ggacgtgccc ctcccccttc caagaggagg ccgcggcccg gacccaggtc acaccctgcg
     4021 agcctgggtc atctcgctac ttcaccggtg tgaagtcccg ggcaccgagg aactcgggcc
     4081 ggcggatggg ggccgcgaag ggctccaccg cggggttctc cacgctgttg aagacgatga
     4141 agacattgct ccgcgggaac ggggtgatgt tgtccccgga gccgtgcatg cagttgcagt
     4201 cgaaccaggt cgccgaaccg gccttgccgg tgaacagctt gatgccgtgc ttctcggcga
     4261 agccggacag cgcctcgtcg gacgggatgc ccgcgtcctg catctgcagc gaccgcttgt
     4321 agttgtcctt cggggtctcg ccggcgcagc cgaggaacgt cttgtgcgaa cccggcatga
     4381 tcatgagacc gccgttggtg tcgtagttct cggtcagcgc gatcgagacg gacaccgtgc
     4441 gcatgttggg caggccgtcc tcggcgtgcc aggtctcgaa gtccgagtgc cagtagaacc
     4501 cggaggcacc gaaccccggc ttgacgttga tccggctctg gtggacgtag acgtccgagc
     4561 cgaggatctg gcgggcgcgg tccagcacgc gcgggtcgcg gaccagcccg gcgaagacct
     4621 cgctgatctt gtgcacctcg aagaccgacc ggaccttctg cgacttgggc tcgacgatcg
     4681 aacgctcgtc ggccttcacc tcgggatcgg tgaccagccg gttcagctcg tcgcggtaga
     4741 ccgcgacctc gtcaggggtg atcagctggt cgaccgccat gaagccgtca cggtcgaagt
     4801 cgctgatttc gttctcggag atcgggccgg gggttccggg agccgaccac acgaccgggt
     4861 ccacccgcgg ggtggccacc tccgtggcgc cgcgggtcgg gtagagatcg gtcgtccgct
     4921 cgggtgcgat ggtcatggtg ctgcaccttc tcctctcgtt acgtccctgc ttctgtccgt
     4981 ttccctcgtc ggggggccgg tcagccctct tcgggctcgg tgagcagcgg gtagacaccg
     5041 ttctcgtcgt ggtcctcacg gccggtgacc ggcgggttga agacgcagac gcaacggaaa
     5101 tccgtcttcg gccgcatcgt gtggcgctcg tgcccgtcca gcagatacat cgtgccgggc
     5161 tcgatccagt gcttctcgcc cgtttcctcg ttggtgagtt cggcctcacc ctccacgcac
     5221 agcaccgcct cgacgtggtt ggcgtaccac atcttggtct cggtccccgc gtagagcgtg
     5281 gtctcgtgga gggagaagcc cacccgctcc ttggcgagga cgatgcgctt gctctcccag
     5341 gtgcccgact gcgacttgat gtgccggtcg gtcccctcga tgtccctgag ggatcggacg
     5401 atcacggtgt tctgcctttc ttttctacgg tcggcgatac gcgggcccgg ggcaacccgg
     5461 caaacaagcc ggattgcccc ggggagaggg ctcagaccgt ctcgcgtacc gcccgggcga
     5521 ggatccgcag accctcgtcc agctcttcgg gggtggtggt cagcgcgggc agcagcttga
     5581 cgacctcgct ctgcggcccg gacgtctcga ccagcagccc cagctcgaag gcgcgcttgc
     5641 acacggcgct cgcgcgggac ttgtcggtga actccatgcc ccacaccaga ccgcggccgc
     5701 gcacgacgcc gccgagccgc gggtgctcct cggtgatcgc cgtgagcgcc tgctcgacct
     5761 gctcgccgcg gctgatggtc tgcttctcca tctggccgtc ggcccagtag gtgtccaggg
     5821 ccgcggcggc cgtcacgaag gccgggttgt tgccgcggaa ggtgccgttg tgctcacccg
     5881 gctcccacac gtccagctcg ggcttgaaca gcgtcagcga catgggcatc ccgtagccgc
     5941 tgatggactt cgacagcgtc acgatgtccg gcgtgatgcc cgcctcctcg aaggagaaga
     6001 aggcacccgt acggccgcag cccatctgga tgtcgtcgac gatcagcagc atgtcccggc
     6061 gccggcacag gtcggccagg gcgcgcagcc agtcggcgcg ggcgacattg atgccgccct
     6121 cgccctggac cgtctcgacg atcacggcgg cgggctggtt gagcccggaa ccctggtcct
     6181 ccagcagccg ctcgaaccac aggaagtccg ggaccttgcc gtcgaagtag ttgtcgaacg
     6241 gcatcggggt gccgtgcacc agcgggatgc cggcgccggc gcgcttcatg gagttgccgg
     6301 tgacggccag cgagccgagg gacatgccgt ggaaggcgtt ggtgaaggag acgatcgcct
     6361 cgcggccctt caccttccgg gccagcttga gcgcggcctc gaccgcgttg gtgcccgtcg
     6421 ggccggggaa catgaccttg tacggcaggt cgcgcggccg caggatcgag ttctggaagg
     6481 actccaggaa ggcccgcttg gcggtggtgc ccatgtccag gccgtgcgtg acgccgtcgc
     6541 gctccaggta gtccagcagc gcgcgtttca gcacctcgtt gttgtggccg tagttgaggg
     6601 ctccggcgcc ggagaagaag tccaggtagg tgtggccgtc ctcgtcgtac atgtagctgc
     6661 ccttggcccg gtcgaacacg gccggccagc cgcggcagta gctgcgaacc tcggattcga
     6721 ggctctcgaa gacactcagg gcgggcgggg tgatggtcac agcgaactcc agggagtggt
     6781 ctggggagtg aggtaagtcg gtgggggcgg gcagggctcg gagtcggggt ttccgggggc
     6841 ccggggtgag ggggcccggg tcagcgcggg ggcgccgtcc acggcagggg tcctatccgg
     6901 tacagcacct cgggcaggtg cccctcctcg gggaacaggc ccgggtcgaa caggacctcg
     6961 cgctccacgg ccgcgccgtg ccgctcggcg aacgaggtga acaggcggtt cgacgccgtg
     7021 ttgtccgggg tgatcgtggt ctcgacccgg tcgatgtcct tctcgtgggc gacccgctcg
     7081 gtcagcccgt cgagcagggc gcccgccagg ccgcgcccgc gctggctctc gtcgacggcg
     7141 acctgccaga cgacgagggt gcggggggcc tcggggcgga ggtacccggt cacgaagccg
     7201 accggctcgc cgtcggcgcc gcgggccacc acggaggtgg cggcgaagtc acggcaccac
     7261 aggaggtagc tgtacgacga gttgaggtcg agcacctcgg agtcgcgggc gatgcgccag
     7321 atcgcggctc cgtcgtcgac gcgtggcgtg tcgagcttga gtccttccgg catttccaag
     7381 aattccgtac ggggacctgc gtggtctgct tgcgcggcgg tcatgcgtat tcaatttacc
     7441 gagcaaaagt taaaaatgca tcggggcaag gggttacgcg gacgggggtt cctgtgttat
     7501 tcgggcgcgg ccgtgcgggc gcgcgaaacc ccgcctgatt gcccgtatat atgggctgat
     7561 tcccggatga accgggcggc ctgtggatcg cgtcacatat ccgtaacaac ccgggccgcg
     7621 gccacgggag aggctcctga ggtctgtggc gaatccgtgt gtttgagaca cgggaaagcg
     7681 ggaagaagag ttccggactc tacgcatttg gagaatacgg aattccgttg aggagaacat
     7741 agcgcgaatg cgcgaaactg tcaccggaat cacggaaccc gaccgccccg gactccgtcc
     7801 gccccctcgc ggacccggcg cgggccgggc ccgcccctcc cgcgtgccac gccgctcgta
     7861 gagtgcctgc catgagcgaa ggcacggtgc tgcacatcaa gggacgggtg ctccgcggcc
     7921 cggaggacgt ccgggacgag ctgtgggtgc gggacggccg cgtcacctac gagccccccg
     7981 ccgccgcgcg gaaggtgacc accctcaccg gctgggtcct ccccggactc gtcgacgccc
     8041 actgccacgt cggcctggac gcccggggcc cggtcgacga gtccaccggc gagaagcagg
     8101 ccctgaccga ccgcgacgcc ggaacgctgc tgatccgcga cgccgggtcg ccctccgaca
     8161 cccgctggat cgacgaccgc gaggacctgc cccggatcat ccgcgccggc cggcacatcg
     8221 cccgcacccg ccgctacatc cgcaactacg cccatgagat cgagccgggc gacctcgtcg
     8281 cgtacgtggc gcgggaggcg cgccggggtg acggctgggt caagctcgtc ggcgactgga
     8341 tagaccgctc caccggcgac ctcgccccct gctggccgcg cggcgaggtg gaggccgcga
     8401 tcgccgaggc ccaccggctc ggtgcccgcg tcaccgccca ctgcttcgcc gaggactcgc
     8461 tgcgcgacct ggtggaggcg ggcatcgact gcgtcgagca cgccaccggg ctgaccgagg
     8521 agaccattcc gctcttcgcc gagcgcggtg tggccatcgt ccccaccctc gtcaacatcg
     8581 ccaccttccc ccggctcgcc gacggcggcc aggagaagtt cccccgctgg tcggaccata
     8641 tgcggcggct ccacgagcgg cgctacgaga ccgtggccgc ggcccacgac gccggcatcc
     8701 cggtcttcgc cggcaccgac gccggcggct ccctcccgca cggcctgatc gcgcaggagg
     8761 tcgcggagct ggtgaaggcc gggctcccgg cggcgaccgc gctgtcggcg gccacctggg
     8821 gcgcgcggga atggctcggg cgccccggcc tgagcgaggg cgcccccgcg gacctggtgg
     8881 tgtacgacac cgacccgcgc gaggacgtcc gcgtcctggc ggcgccccgc cacgtggtgc
     8941 tgcgcggccg cgtcaccggc tgagccgccc tacgccgccg tacgccgccg tacggggacc
     9001 cgccgccgcg ggtccccggg ccggggagtc gaacgcacgc ccagaaacca cccgatggtg
     9061 tgaactcacg ccatgttgct gcctgttcac cgacagtacg taagtatgcg ttgcgtacga
     9121 ggtcgccggg cggacgtctc ccggaccgcg ccggtgactc catttctctt gtgggggttc
     9181 cacaccttga acagcaatgc cttcgtcccg cccgtgcgcc gtaccgccgc cgcgctgacg
     9241 gccgccgctc tggccgccgc cccggtgctc ctcgccggtg ccgcgcccgc gtacgcctcc
     9301 ggaggggacg gcaaggccgg agcggtcgtg ctccgcgcgg acctcggggt caccctgctc
     9361 gaccagaccc gggtgccgct gaacgtcacg ctcaacgagg tccaggcccc ggagtcggcc
     9421 agtgagacgc tgctgaccgc gcgggtggag ggggcccacc ggggccgtcc cgtcgaggtg
     9481 ctgaaggcgg ccgtcgcgac cgcggaggcg aagacggagg ccgggaaggc cgaggggcgg
     9541 gccaacctcg tgaacgcccg ggtgttcgtg cccggtctga agaagcaccc gctgatcgag
     9601 ctcgaccagg tcacgtccac cgccgtgtgc ccggccggcg ggcagccgac ggccgaggcg
     9661 aagattcccg ggcatgtgac ggtcttcggc cagcgggtcg agctgagcgc cgagggcacc
     9721 gcgcgggcgg acgtcgaggg catcggatcg gtcgagctgg ccctggcgaa gaccaccacc
     9781 acggcgacga ccgccgcggc cacggccctg gagctccagg tgtccgtcac cccggaactg
     9841 accgcgccga agaagctcaa cgtcgcggcc gtggagggca aggtcaccct ggtggaggcc
     9901 acctgtgaga gccccgccgg cggctccggc gacggcgggc cgggcaccgg cagcggcgga
     9961 gcgaccgagg gcgccaccgg cggctcgggc ggctcgggcg acggttcggg cgacggcggc
    10021 tcgggtgacg gttccggcga cggctcgggc gacggctcgg gcgacggctc gggcgacggc
    10081 gtcagcaccc agaccggctc cggcagcggc gaggacaacc tcgccgccac cggcggcaac
    10141 tccaccactc cgtacctggc ggccggtgcc ggggccctgc tcgcggccgg cggagtctcg
    10201 gtcttcgtcg cacggcgccg ggcggccgcc cgccgcggct gaccgcgacg ggtccggggc
    10261 cgccggtccc gggcccggac cggaaaggtg acggcgcggg ccgccccggg ggtggggcgg
    10321 cccgcgccgt cggccttccg ccgccgtccg tacctcccgg cccggccgcg cgggaccgcc
    10381 cgtacggcgg ggtgcccgcg aggcc
//
