LOCUS       NZ_CP023202            12844 bp    DNA     linear   CON 21-MAR-2024
DEFINITION  Streptomyces xinghaiensis S187 chromosome, complete genome.
ACCESSION   NZ_CP023202
VERSION     NZ_CP023202.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces xinghaiensis S187
  ORGANISM  Streptomyces xinghaiensis S187
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7137891)
  AUTHORS   Zhao,X. and Yang,T.
  TITLE     Draft genome sequence of the marine sediment-derived actinomycete
            Streptomyces xinghaiensis NRRL B24674T
  JOURNAL   J Bacteriol 193 (19), 5543 (2011)
   PUBMED   21914869
REFERENCE   2  (bases 1 to 7137891)
  AUTHORS   Chen,L., Zhao,X. and Lin,S.
  TITLE     Genome mining of a marine derived strain Streptomyces xinghaiensis
            reveals biosynthetic ability of xiamycin analogues
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7137891)
  AUTHORS   Chen,L., Zhao,X. and Lin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2017) School of Life Science and Biotechnology,
            Dalian University of Technology, Linggong Road 2nd, Dalian, Liaoning
            116023, China
REFERENCE   4  (bases 1 to 7137891)
  AUTHORS   Zhao,X., Yang,T. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2011) School of Life Science and Biotechnology,
            Dalian University of Technology, Linggong Road 2, Dalian, Liaoning
            116024, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2017
            Assembly Method                   :: Canu v. 1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: Streptomyces xinghaiensis S187 genome
            sequencing
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000220705.2-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 05:53:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,157
            CDSs (total)                      :: 6,072
            Genes (coding)                    :: 5,922
            CDSs (with protein)               :: 5,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 36 of 150
            Pseudo Genes (incomplete)         :: 134 of 150
            Pseudo Genes (internal stop)      :: 6 of 150
            Pseudo Genes (multiple problems)  :: 26 of 150
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:47:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 955178
            Orig. end                         :: 968022
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023202.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Shanghai Jiaotong
            University, School of Life Science and Biotechnology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12844
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="7"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12844
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12844
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7844](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7844
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(1049..1058)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     misc_feature    1509..1518
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.11"
                     /tool="antismash"
     gene            1669..2834
                     /locus_tag="SXIN_RS04075"
     CDS             join(1669..1941,1944..2834)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611786.1"
                     /locus_tag="SXIN_RS04075"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS3 family transposase"
                     /protein_id="WP_238153667.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MPAPYPEEFRDDVVRVARNRGKGVTLAQIAKDFGVHEMTLSKWLR
                     RAAVEDGEKPGVTRSESAENRELKKRVRLLEQENEVLRRAAAYLSQSSSGKRLYPLVRD
                     LAADGIPVAVACRVLGLARQHYYRWLRRPVSGAEVTEAYRANTLFDAHGDDPEFGYRFL
                     LGEAETAGERMCERTAWRICRDNAWWSVFGKKRSKNGKRPGPPVHDDLVRRDFTANAVN
                     ELWLTDITEHPTGEGKLYLCAVKDACSGRIVGYSIGSRMQARLAVTALDNAVARRGVVA
                     GCVVHSDRGSQFRSRRFVHALHRHGLVGSMGRVGAAGDNAAMESFFALLQNNVLDRRTW
                     TTRQDLRIAIVTWIERTYHRRRRQPRLGRLTPIEFETTMSPATTLAA"
     gene            complement(3302..4885)
                     /locus_tag="SXIN_RS04080"
     CDS             complement(3302..4885)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639390.1"
                     /locus_tag="SXIN_RS04080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_039823042.1"
                     /sec_met_domain="Aldedh (E-value: 4.4e-49, bitscore: 165.7,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLTGSSIVAGRDVPGTGETWRSIVAATAEPQGPRLRDASPDQVG
                     QAAQAASSAARDYRALPVHRRAAFLDACAEEIEALGDTLLRLAASESGLPLDRLAGERG
                     RTCGQLRMFADLVRSGDALGVRVDPALPERQPLPRADLRLRRIPLGPVAVFGASNFPLA
                     FSVAGGDTASALAAGCPVIAKAHPAHPGTCELVARAVTAAGERTGMPAGVFSLLVGRGT
                     QVGQALVRDPRITAVGFTGSRSGGLALVEAANARPVPIPVHAEMSAVNPVIVLDGALSD
                     PAPLATAYVSSLTNGSGQFCTNPGLLLLPSGTAGDAFCTAVAQAVAGTTGQVMITPGIA
                     EAYTRGTGRWAAVDGVSVVAQGVPGESPYAPSSAVLECDAATYASHEDLSGEVFGAAGL
                     VVRWSGADELLALLEQIEGQLTATLHGTDADLATAERVLPVLEERAGRILWGGWPTGVE
                     VCHAMVHGGPWPATSSPGTTSVGTLAIERWLRPVCYQSFPDALLPEELRAGNPLGVTRL
                     IDGVLEPRSR"
     misc_feature    4954..4974
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.85"
                     /tool="antismash"
     misc_feature    complement(4955..4975)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.71"
                     /tool="antismash"
     misc_feature    complement(4977..4987)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            5001..6167
                     /locus_tag="SXIN_RS04085"
     CDS             5001..6167
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972636.1"
                     /locus_tag="SXIN_RS04085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_019710708.1"
                     /sec_met_domain="DAO (E-value: 4.1e-69, bitscore: 232.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLKVLVVGAGVVGAACAFHASLAGLDVTIIDRGPVGAGTTSRGE
                     GNILLSDKEPGPELELAHLSRALWDEAGEELGADSLEFESKGGLVVASTDEGLPALRAF
                     AERQAAAGVLIEPVDRVHDLEPHLSPGIPGGVHYPQDAQVQPVLAAAALLRAAVARGAR
                     FHTGEVAAAVTDRDSRVTGIRTTAGDVLPADAVVNAAGTWGGEVGRRLGAPIEVLPRRG
                     FVLVTEPLPPMVRHKVYSADYVANVASSDAGLETSCVVEGTRGGTILIGASRERVGFDT
                     TMNPVVVPKLAAQACRLFPFLSEVHVIRAYRGFRPYCPDHLPVIGPDPRVPGVVHACGH
                     EGAGIGLAPATGALVTAHLLGRPWHGADPAAHAGLLPDRFFTSGGGSE"
     gene            6164..6418
                     /locus_tag="SXIN_RS04090"
     CDS             6164..6418
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972635.1"
                     /locus_tag="SXIN_RS04090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_019710709.1"
                     /sec_met_domain="Fer2_4 (E-value: 9.2e-25, bitscore: 85.0,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEIIVDGRPLAFIEGQTVAAALVAAGRVAWRTTRIGHRPRGVFC
                     GIGVCFDCLVTIDGAGGQRACLVPARPGMTVTTGEGDDE"
     gene            6411..7844
                     /locus_tag="SXIN_RS04095"
     CDS             6411..7844
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030798.1"
                     /locus_tag="SXIN_RS04095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_039823045.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.5e-22, bitscore:
                     76.8, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.1e-10, bitscore:
                     40.3, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSESADLIVVGAGPAGMAAAVTALAGGLRVILVDSGTAVGGQFWR
                     HPPEHARSAIRTEDLHHKLRDYHALSRALSAGRAKGRLGLRLAHHVWSAVREGDGFAVR
                     AVDRREVPEETAVVLRAPRLLVATGAYDRQLPFPGWDLPGVLTVGGLQALLKGGGVAAG
                     SRVALGGTGPFLLPVAAGLAKRGVEVVAVCEAAHPLAWLRHPGPLLGNPGKWVEGARYA
                     AALARHGIRVRPRTAIVGAEGGDRVTSVRIASLAHDGSPRPGTERRVEVDAVGVGWGFT
                     PQLDLLLPLGCELTGSEDGNAVVAVDDGQRTTVPGLYAAGEACGVGGAPLAVGEGRLAA
                     ESVLADSGAAPVLGKKRLARVRATVARQRAFARAMSLAHPIPPAWSSWLTDETVVCRCE
                     EVTAGAVRAACTGSGASDHRQVKQLTRAGMGWCQGRMCGPAVHCLASQERAYVPAERLV
                     ATPVTLGALGDFEKYSENR"
     gene            7855..8751
                     /locus_tag="SXIN_RS04100"
     CDS             7855..8751
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030799.1"
                     /locus_tag="SXIN_RS04100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_019710711.1"
                     /transl_table=11
                     /translation="MSETRKPWHGVIVATSLPFNDDLTVDFGAYGESVSWLAEQGLHGV
                     APSGSLGEYQTLTYEERDRVVEAAVANAPEGFTVMPGVGAYGGGEAQRHAQFAKDAGCQ
                     AVMCLPPNAYRADDRAVLEHFELVASVGLPVTAYNNPVDTKVDLRPELLAKLHAEGFIV
                     GVKEFSGDVRRCYEISELAPGLDLMIGTDDTVLEVGIAGAKGWVAGYPQVFPQACLALY
                     NASVTGDLETALPLYRQLHPVLRWDSKTEFIQAIKLGQEMIGRRGGACRPPRQALSPET
                     EAVVRGATQALIDAGVN"
     gene            8754..9755
                     /locus_tag="SXIN_RS04105"
     CDS             8754..9755
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378674.1"
                     /locus_tag="SXIN_RS04105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_039823048.1"
                     /transl_table=11
                     /translation="MRSTVCYHAVDSHTEGMPTRVITGGVGVIPGATMFERRQRFVAER
                     EDLRTLLMYEPRGHASMSGAILQPPTRPDADFGVLYIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVAEPETLVRLDTPAGLVTARVRVRDGHAESVTLENVASYCHALDQVVDVEGYGP
                     VRYDIAYGGNFYAIVRLEDLGIPFDRAEKQRLLDAGLSIMKAINTQNPVAHPENPQINV
                     CHHVYLEAPGSTAEHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARRQLLVGQEFVNES
                     FIGSRFVGQVLGETTVGGRPGILPSVTGRAWITGTAQYMLDPSDPYPAGFTL"
     misc_feature    9810..9824
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     misc_feature    9862..9872
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     misc_feature    9964..9974
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            10172..11323
                     /locus_tag="SXIN_RS04110"
     CDS             10172..11323
                     /GO_function="GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1282:
                     extracellular ligand-binding receptor"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003404.1"
                     /locus_tag="SXIN_RS04110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_019710713.1"
                     /transl_table=11
                     /translation="MTLIRRGTMAAAAMALGLTITACGGSLTQDNSGGDSSGTIKLGML
                     TPLTGSSAAIGPYMKDGAQLAVDEINAKGGVDGRKLSLTVEDEACDPKTAAAGAAKLVS
                     AGIDISVGGYCSSATLPTLSIFGKAHIPMLIPAANSADLVSQKQQNVFLLNGTGIQQAE
                     AASKFIKAAGSKGVALVDDNTSYSTDITKRTEEGLKQSGVEVVNHQSVTAGESDYSGAI
                     NAVLDSKADFIYWTGYYQEGGLLIKQLKAAGYKGEIMVADGSVDAKLIQIAGQKNAQGV
                     FATMTQTPQTIPGGEAWVAKYKEKFGSEPGPYSTQAYDAVRVAAEAVRKAGSTDGQAVT
                     SALEQIDGLEIFSGPLKFTAQHTLSAGGFDILVVRDDNFVLNK"
     gene            11400..12299
                     /locus_tag="SXIN_RS04115"
     CDS             11400..12299
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003403.1"
                     /locus_tag="SXIN_RS04115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="WP_019710714.1"
                     /transl_table=11
                     /translation="MDHFAQLVLSGLFVGSFYALVALGYSMVYGIIKLLNFAHGDIYML
                     GAFAGFTVLSVGAGALGGGFFALITIMIVAMIATGTFGMVLERTAYRPLRSAPRLSVLI
                     TAVGASFALEYGIAVAFGPTPEVYQIGIHGGTLDLAGARMTYAQLAMMAIAVVLMIALD
                     TLVRKTRSGRAMRAISQDPKACLLMGINVDRVISRTFFLGSALAGAAGVMAGAYYGKID
                     FLMGFIIGLKAFTAAVIGGIGNLRGAVLGAVVLGLLESFGVAYLGSEWRDVFAFGFLIL
                     FLTVRPTGLLGERVTERV"
     misc_feature    12407..12422
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.22"
                     /tool="antismash"
ORIGIN
        1 ctccggcgac cggaccgctg tccttggcga tgttgtggaa gaaggcgtcc tggccggcca
       61 ggctgctgca ggtgctactg agctggcccg ccgtcatttc ctgcgccttg atgcgcaggc
      121 tcgagctgca ggtgtggttg atggggagga cttctgcggc caggcgctgc tgcaggttgc
      181 aggtgccgta ggaggagcag ttggccttgt cgtagtagtc cgtcatctcc gcgacgccga
      241 cccacagcgg cgccgtcctg ccagtgatgg cgctctggtt gagcaggctc gtggcgtacg
      301 ggcggacctt ggagtggagt tccgtgtgct gcaggaagcg tccgagttcg cgtcccgcgt
      361 tggaggtgag gtagccctgg tcggtgccca gcagaccggt atgcgaggac ccgaaggtgt
      421 acaccgtgtc gatcacgctt gggtcggcct tcaccgcggt gacgaacgcg ggcacctggt
      481 ggccccggaa gagcaccgta tacacgttgt tgacggcgtt cagcatccac caggaggcgt
      541 tgtaggagct gttgtagtcg ttcagcagcc gcttgaccac atacaggtag cgagcgttct
      601 cctcggcgct gtcgatcagc gtgacggcct cggcgagggt ttccccgttg gcgtcggtga
      661 cgtccctgga gtgagtgctg gcgaagaagg cgtcgaggcc cgaccgtatc gctgtctgaa
      721 gattgggccc gtaggtgccc acggttgagg cgttgtacca gtgtacgtag tagcccgccc
      781 gcaggtacag caccagctgc ggcatcccgg tgctgctgtt gcccgggtac gcggatgagc
      841 cgtccctgac cgcataggcg acgctggcca tctgcgactc gcggaaggcg tagtaggcgt
      901 cgctgccggt cagattgaac agcgtattga tgcagtccgt ggtcgatgcc ttgatctgct
      961 ggacgagcgc ggcgccggaa cggctggtga agtcactggt gctgcacgcg gtcgcggcgg
     1021 agggtgagag cttcccctcc ttctcctgcg ccgccttcat cgacgggctg acccgcggag
     1081 ccggcgaggc cggatcgtcg tagtccctct tgagcgcatc cttcgttgcg ggcaggggcg
     1141 cgcggtccgc cggcttcagc ggcgccttcc ggacgtgttc ggcctcctca cggtcctgcg
     1201 tggccgaaga ggcctgcggc ggcgggtcct ttggcgcctt ctcggatgtg gtcgccgttg
     1261 ctgttgccgc ccggccgggc gcggagagca gcccgacccc caagcacagt gtcagcacca
     1321 ggacgagaat cctggctacg tcctgtcgca cctgtttcgt tctcacgtgc cgcctccagc
     1381 ggtgggtgag gccacgccgg gggcgtggcc tgagtgagag ttgtcctgcg cggttccggg
     1441 gcggtcgccc cgagaggttt ggcatgaaca cgcgcgtgcc atgcgacagt acaatggcac
     1501 acgtcacatg tttcttgaag acctcgtact gaggaaaacg cccccaactc acggtgccgc
     1561 aacgggacat gcggcggtct tccacgagga atcacgcaca cgggtctgct gcacggatag
     1621 gtgacatctg atctggcttg cctgtgggcg gcctggaagg atgttgctgt gcctgctccc
     1681 tatcccgaag agttccgtga tgacgtcgtg cgtgtggccc gcaaccgcgg gaagggtgtg
     1741 accctcgccc agatcgcgaa ggacttcggc gtccatgaga tgacgctgtc gaagtggctg
     1801 cgccgggccg ccgtggaaga cggcgagaag cccggtgtca ccaggtcgga gtcggcggag
     1861 aaccgtgaac tgaagaagcg ggtacgactg ctggagcagg agaacgaggt cctgcgccgg
     1921 gctgcggcgt atctgtcgca ggctcatctt ccgggaaaag gctctacccg ctcgtgagag
     1981 acctcgccgc cgacgggatc cctgtcgcgg ttgcgtgccg ggtcctgggg cttgcccgcc
     2041 agcactacta ccggtggctt cgccggccgg tgagcggggc cgaggtcacc gaggcctacc
     2101 gcgcgaacac gctgttcgac gcccacggtg acgacccgga gttcggctac cggttcctct
     2161 tgggcgaggc cgagacggcc ggcgagcgga tgtgtgaacg gaccgcgtgg cggatctgcc
     2221 gggacaacgc ctggtggtcg gtgttcggga agaagcggtc gaagaacggc aagcgccccg
     2281 gaccgccggt ccacgacgac ctcgtccgcc gggacttcac cgcgaacgcg gtgaacgagt
     2341 tgtggctgac cgacatcacc gagcacccga ccggtgaagg aaagctgtat ctgtgcgcgg
     2401 tcaaggacgc ctgctccggg aggatcgtgg gctactcgat cggcagccgg atgcaggcga
     2461 ggctcgcggt cactgccctg gacaacgccg ttgcccgccg aggtgtggtg gctggctgcg
     2521 tcgttcattc cgatcgcgga tcgcagttcc gctcacgccg gttcgtccac gctctgcacc
     2581 gtcatggcct ggtcggatcg atgggcagag tcggcgccgc cggcgacaac gcagccatgg
     2641 aatccttttt cgctctcctg cagaacaacg tcctcgaccg ccgcacctgg accacccgcc
     2701 aagacctgcg gatcgcgatc gtcacctgga tcgaacggac ctaccaccga cgccgccgac
     2761 agccacggct gggccgattg acccccatcg agttcgagac caccatgagc cccgccacga
     2821 ccctcgcggc atgaaacccc actgtcacca aaccgtgcag cagaccccac cgaccggcat
     2881 ccctgaccgc tcgggcccct gtggtgggcg ccctaccgcg cctgagcttc acccggcttg
     2941 gcgagagcag gtcggcaggg gtgggcgctg ccgtggaggt caccggcgag gctccatcac
     3001 aagccgggca tgcctgaccc gggccgggtg ccgcgtcgac gtgaggccga caagccgccg
     3061 ctgcacgagt tgccgcgcac cccggaagcc acggccgcta tgtccggaac cgccctaccg
     3121 gccgccccgg acaacaagcg gcccgtgact gcgaggccct ggcccgtgga aacgtccctc
     3181 tcactgccag gacgacgggc tgcaagccgg taaccgcctt gagtcccgtg gcgcgggcaa
     3241 cctcttcaaa ggctcgcatc cctgtatgtg tccggcgccg cggcaggagt tcggtgtgcc
     3301 gttacctgga ccgcggctcc agcacgccgt cgatgaggcg ggtgacgccc agcgggttgc
     3361 cggcacgcag ttcctcgggc aggagtgcat cggggaacga ctggtagcag acgggccgga
     3421 gccagcgttc gatcgccagg gtcccgacac tggtcgtacc gggcgaggac gtcgcgggcc
     3481 acggaccacc gtgcaccatg gcgtggcaga cctcgacccc cgtcggccaa ccgccccaca
     3541 ggatgcgccc ggctcgctct tccagcaccg gcaggacccg ctcggccgtc gccaggtcgg
     3601 cgtcggtccc gtgcagggtg gccgtcaact gcccctcgat ctgttccagc agagcaagga
     3661 gctcgtcggc cccggaccac cgcacgacga gtccggcggc accgaagacc tcacccgaga
     3721 ggtcctcgtg gctcgcgtac gtcgccgcgt cgcattccag gaccgcggag ctcggcgcgt
     3781 acgggctctc accgggcaca ccctgtgcca ccacggagac accgtcgacg gccgcccacc
     3841 gcccagtccc cctggtgtac gcctctgcga tccccggggt gatcatgacc tgtccggtcg
     3901 tgccggccac ggcctgggcc acggccgtgc agaaggcgtc acctgctgtg cccgacggca
     3961 gcagcaggag accggggttc gtgcagaact ggcctgaccc gttggtcagt gacgacacgt
     4021 acgcggtggc aagcggtgcg ggatcggaca gggcgccgtc aaggacgatc acagggttga
     4081 ccgccgacat ctcggcatgg accgggatgg gcacgggtcg ggcgttcgcg gcctcgacca
     4141 gcgcgagccc tccgctccgg gaaccggtga atccgacggc tgtaatccgc ggatcgcgca
     4201 ccagcgcctg gccgacctgg gtaccgcggc cgaccagcaa cgagaagaca ccggcgggca
     4261 tgccggtgcg ctcgccggca gcggtgacgg cacgggcgac gagttcgcat gtgcccggat
     4321 gagccgggtg cgccttggca atgaccgggc agccggcggc gagcgcggag gccgtgtcac
     4381 cgccggcgac ggagaaggcc aacgggaaat tgctcgcgcc gaacacggcg acgggaccga
     4441 gagggatcct gcgcaggcgc aggtccgctc gaggaagtgg ctgacgctcg gggagcgcgg
     4501 ggtcgacacg gactcccagc gcatccccgc ttcggacgag gtcggcgaac atgcgcaact
     4561 ggccgcaggt gcgtccgcgt tcgccggcca ggcggtccag cggcagcccg gactcgctgg
     4621 ccgcgagccg caggagggtg tcgccgagtg cctcgatctc ctccgcgcag gcgtcgagga
     4681 aggcagcccg ccgatgcacg ggcagggcac ggtagtcgcg cgcggccgag gaggccgcct
     4741 gggctgcctg cccaacctgg tccggcgagg cgtcccgcaa ccggggtccc tgcggctcag
     4801 ccgtggcggc cacaatgctc cgccacgttt ctcccgtccc gggaacgtcc cggccggcca
     4861 cgattgagct tcccgtcagg ttcacagttc tccatccctc gcccgtgcag gacaaaagtc
     4921 atatcggtgt ggtcgttgtc tcagctcgct ccgacgtata gcctaggcaa taacatgtga
     4981 cattgcacag aggagagccg atgaccctga aggtcctcgt cgtgggcgcg ggtgtcgtcg
     5041 gcgccgcctg cgccttccac gccagcttgg cgggcctcga tgtcacgatc atcgaccgcg
     5101 gccccgtcgg cgccgggacg acaagtcgcg gagagggcaa catcctcctc tccgacaagg
     5161 aaccggggcc cgaactcgag ctggcccacc tgagccgtgc gctctgggac gaggccggcg
     5221 aggaactcgg ggcggactct ctcgagttcg agtccaaggg cggcctggtc gtcgcgagca
     5281 cggacgaagg tctcccggcg ctccgcgcat tcgccgagcg gcaggcggcg gccggcgtcc
     5341 ttatcgagcc ggtcgatcgc gtacacgacc tcgaacccca cctctcacct gggatccccg
     5401 gcggggtcca ttacccgcag gatgcccaag tgcagcctgt tctcgcagcg gccgcactgc
     5461 tgcgcgccgc cgtggcgcgt ggcgcgcgct tccatacggg cgaggtcgcc gccgcagtga
     5521 ccgaccggga cagcagggtc accggcatcc ggacgacggc cggagacgtc ctgcccgccg
     5581 acgccgtcgt caatgctgct gggacgtggg gcggtgaggt cggccgccga ctgggtgcgc
     5641 ccatcgaggt gctgccacgc cggggcttcg tcctcgtgac cgaaccgctg ccaccgatgg
     5701 tccggcacaa ggtctactcg gccgactacg tcgccaacgt cgcctcgtcc gacgccggac
     5761 tggagacctc gtgcgtcgtc gagggcacgc ggggcggcac gatcctcatc ggtgccagcc
     5821 gggaacgcgt cggcttcgac acgacgatga atcccgtcgt ggtccccaaa cttgccgcgc
     5881 aggcgtgccg gttgttcccc ttcctgagtg aggtccacgt gatccgcgcc taccgcgggt
     5941 tccggccgta ctgcccggat cacctgccgg tcatcggtcc cgacccgcgg gtcccggggg
     6001 ttgtccatgc atgcggtcac gagggcgcgg gcatcggtct cgcccccgca acgggtgcgc
     6061 tcgtgaccgc ccacctgctg ggccgtccct ggcacggcgc cgatccggca gcgcacgccg
     6121 gccttctgcc cgaccgcttc ttcacctccg gaggtgggtc agagtgagcg agatcatcgt
     6181 cgatggaagg ccactggcgt tcatcgaggg acagaccgtt gccgcagccc tcgtggccgc
     6241 gggccgggtc gcctggcgca ccacccgcat cgggcaccgc ccgcgcggcg tgttctgcgg
     6301 gatcggcgtc tgcttcgact gcctggtgac gattgacgga gccggtgggc agcgtgcctg
     6361 tctggtaccg gcgcgtccgg gcatgaccgt cacgaccggg gagggtgacg atgagtgaat
     6421 cagcggatct cattgttgtg ggtgcgggtc cggccggtat ggccgcggcg gtcacggctc
     6481 tggccggcgg gctgcgcgtc atcctcgtcg actcgggcac cgccgtcgga ggtcagttct
     6541 ggcggcatcc gcccgagcac gcgcggtcgg cgatccggac agaagatctc caccacaaac
     6601 tgcgggatta ccatgcgctc tcccgtgcgc tgtcggcagg ccgggcgaag ggccggcttg
     6661 gactgcgcct ggcgcaccac gtgtggtccg ccgtccgcga gggcgacgga ttcgcggtcc
     6721 gcgcggtcga ccggcgcgag gtgcccgagg agaccgccgt ggttctgcgc gcaccgaggt
     6781 tgctcgtggc caccggggcc tacgaccggc aactgccgtt ccccgggtgg gacctgccgg
     6841 gtgtactcac cgtcggcggc ctccaggcgc tgctcaaggg cggcggagtc gcggccggga
     6901 gccgcgtggc gctgggcggc accggcccgt tcctgctgcc ggtggccgca ggcctcgcga
     6961 aacgcggagt cgaggtggtc gcggtgtgcg aggcggccca cccgctggcg tggctgcggc
     7021 acccgggtcc gcttctgggc aacccgggca agtgggttga gggtgcccgg tacgccgcgg
     7081 cccttgcgcg ccacggcatc cgggtccgac cgcgcacggc gattgtcggc gccgaagggg
     7141 gcgaccgggt gacctcggtc cggatcgcct cactggcgca cgacggcagt ccgcgcccgg
     7201 gcacggaacg acgggtcgag gtcgatgccg tcggcgtcgg ctggggtttc acacctcagc
     7261 tcgacctgct cctccccctc ggctgtgagc tcacgggttc cgaggacggc aacgcggtcg
     7321 tcgcggtcga cgacgggcag cgcaccaccg tccccggact ctatgccgcg ggtgaggcgt
     7381 gcggtgtggg cggcgccccc cttgcggtgg gtgaggggcg ccttgccgcc gagtcggttc
     7441 tcgccgattc cggagcggct cccgtgctgg ggaagaagcg tctggccagg gtgcgcgcca
     7501 cggtggccag gcaacgtgcc ttcgcgcggg cgatgagcct tgctcacccg atcccgccgg
     7561 catggtcctc gtggctgacc gacgagaccg tggtgtgccg gtgcgaggag gtgaccgcgg
     7621 gcgcggtccg ggccgcctgc accggcagcg gggcaagtga tcaccggcag gtcaagcagc
     7681 tgacccgggc cggtatgggc tggtgccagg ggcggatgtg cggaccggcg gtccactgcc
     7741 tcgcatcaca ggagcgggcc tacgtgcccg cggaacgact cgtcgcgacg cccgtcaccc
     7801 tcggtgcgct cggcgacttc gaaaaatata gtgaaaaccg ataaaggagc attcatgagc
     7861 gagactcgca agccctggca cggcgtcatc gtcgcgacga gcctgccgtt caacgacgac
     7921 ctcacggtgg acttcggcgc ctacggggag agcgtctcct ggctcgccga gcaggggctg
     7981 cacggtgtcg cgccgagcgg ttcgctgggc gagtaccaga ccctgacgta cgaggagcgc
     8041 gaccgcgtcg tcgaagcggc cgttgcgaac gcccccgagg ggttcaccgt gatgccgggc
     8101 gtcggcgcct atggcggtgg tgaggcccag cggcatgccc agttcgccaa ggacgcaggc
     8161 tgccaggccg tcatgtgcct gccgcccaac gcctaccgcg ccgacgaccg cgcggtcctc
     8221 gagcacttcg agttggtggc ctcggtcggc cttcccgtca ccgcgtacaa caaccccgtc
     8281 gacaccaagg tcgacctgcg cccggagctg ctggccaagt tgcacgccga gggtttcatc
     8341 gtcggcgtca aggagttctc cggtgatgtc cggcggtgct acgagatctc cgaactcgca
     8401 cccggcctcg acctcatgat cggcaccgac gacaccgtcc tggaggtcgg catagccggc
     8461 gccaagggct gggttgccgg ttaccctcag gtcttccccc aggcctgtct cgcgctgtac
     8521 aacgcctcgg tcaccggtga cctcgagacg gctctgccgc tctaccgtca gctgcacccg
     8581 gttctgcggt gggacagcaa gacggagttc atccaggcca tcaagctcgg ccaggagatg
     8641 atcggccgac gcggtggcgc gtgccgcccg ccgcggcagg cactcagccc ggagacggag
     8701 gccgtcgtgc gcggcgccac ccaggccctc atcgacgccg gagtcaactg acgatgcgct
     8761 cgactgtctg ttaccacgct gtcgactcac acactgaggg gatgccgacc cgtgtgatca
     8821 cgggtggcgt cggcgtcatc cccggggcga ccatgttcga gcggcggcag cggttcgtag
     8881 cagaacggga ggacctgcgc accctgctca tgtacgaacc gcgcgggcac gcgtcgatgt
     8941 ccggggccat cttgcagccc ccgacccggc cggacgccga cttcggcgtg ctctacatcg
     9001 aggtctccgg atgcctgccc atgtgcgggc acgggaccat cggcgtcgcg acggtcctcg
     9061 tcgagacggg gatggtcgag gtcgcagagc cggagaccct ggtccgtctc gacacaccgg
     9121 cggggctggt cacggcccgg gtgcgggtcc gtgacggcca cgccgagtcg gtgaccctgg
     9181 agaatgtcgc atcgtactgc cacgctctcg accaggtggt ggatgtcgag gggtacgggc
     9241 cggtgcggta cgacatcgcc tacggcggca acttctacgc catcgtccgg ctggaggatc
     9301 tcggcattcc cttcgaccgg gcggagaagc agcggctgct cgatgccgga ctgtccatca
     9361 tgaaggcgat caacacccag aacccggtcg cccacccgga gaatccccag atcaacgtgt
     9421 gccaccacgt ctatcttgaa gcccccggat cgactgctga gcactcgcgg cacgccatgg
     9481 cgattcaccc gggttggttc gaccggtctc cctgtggcac gggtaccagc gcccggatgg
     9541 cgcagctgca cgcacgaagg cagttgctgg tcggccagga gttcgtcaac gagtcgttca
     9601 tcggatcccg gttcgtcggc caggttctcg gcgagacgac ggtcgggggc cggccaggca
     9661 tcctgccatc cgttacggga cgcgcgtgga tcaccggaac cgcgcagtac atgctcgacc
     9721 cgtccgaccc ctacccggcc ggtttcacac tctgagtgaa ctcgtatgcc gcccacgcgg
     9781 catacgagac ggccaaaagt cacgcgtcta tcacaagtgt cgacaaagct cgcacctcag
     9841 ggcgtcgaca gctatcgttt gtgcaatgtc acacatcaca gcatgtgtgc atggcatcgg
     9901 cccaggcgga gttacgcgcc caccccagcg aaactctcag cccaaaacac ccgcctccat
     9961 ctgtgcaatg tcacacatta cagaggcgtg gcgttccgca gcagatccac gcgccaccac
    10021 aagtgccggc gccttccctt ccacggcccc aagacagcct ctgtgcaatg ccgcacatcg
    10081 catgcacctg cgtggcattg ccgcagccgg ggagcaacct cggccaacgg acggtccgcc
    10141 ccccggcgga tcacgcgaga tggagtaagg catgaccctc attcgtcgcg gcaccatggc
    10201 ggcggccgcc atggcactcg ggctcaccat cactgcatgt ggcggcagtc tcacccagga
    10261 taattccgga ggcgacagtt ccggcaccat caagctcggc atgctcacgc cgctgaccgg
    10321 cagctcggcc gccatcggcc cctacatgaa ggatggagcc cagctcgcag tcgacgagat
    10381 caacgccaag ggcggggtcg acggccgcaa gctctcgctc accgtcgagg acgaggcatg
    10441 cgacccgaag acggccgccg ccggcgccgc caaactcgtc agtgccggca tcgacatctc
    10501 ggtcggtggc tactgttcct cggcgactct gccgacgctg agcatcttcg gcaaggccca
    10561 catccccatg ctcatcccgg ccgccaactc cgcagacctg gtctcccaga agcagcagaa
    10621 cgtcttcctg ctcaacggca cgggcatcca gcaggcggaa gccgcgtcga agttcatcaa
    10681 ggcggcgggc agcaagggcg tcgccctggt agatgacaac acctcgtact ccaccgacat
    10741 caccaagcgg accgaagagg gtctgaagca gtccggcgtt gaggtcgtca accaccagtc
    10801 ggtcaccgcg ggcgagtcgg actactccgg tgccatcaac gcggtactgg actcaaaggc
    10861 ggacttcatc tactggacgg ggtactacca ggagggcggc ctgcttatca agcagctcaa
    10921 ggccgcgggt tacaagggcg agatcatggt tgcggacggt tcggtcgacg ccaaactgat
    10981 ccagatcgcc gggcagaaga acgcgcaggg cgtattcgcg acgatgactc agacgccgca
    11041 gaccattccc ggtggcgagg cctgggtcgc caagtacaag gaaaagttcg gctccgagcc
    11101 cggtccctac tcaacgcagg catacgacgc ggtgcgcgtc gccgcggagg cggtcaggaa
    11161 ggccggctcc accgacggcc aggccgtcac cagcgcgctc gaacagatcg acggccttga
    11221 gatcttctcc ggcccgctga agttcacggc ccagcacacg ctgagcgccg gcggcttcga
    11281 catcctcgtt gtcagagacg acaacttcgt cctgaataag tgacgtcggc cgggtggccg
    11341 gccaacaccg gccggccacc cgcgcgtcgc tgcttccctt cacgcaccgg gaatcttgca
    11401 tggaccactt cgcccaactc gtgctcagcg gcctgttcgt cggctcgttc tacgccctgg
    11461 tcgcactcgg ctactcgatg gtctacggga tcatcaaact gctcaacttc gctcacgggg
    11521 acatctacat gctcggagcg ttcgccggct tcaccgtgct cagcgtcggt gccggtgcgc
    11581 tcggcggtgg attcttcgcg ctcatcacca tcatgatcgt cgcgatgatc gctaccggta
    11641 ccttcggcat ggtcctggag cggacggcct accgcccgct gcggagcgcg ccccggctgt
    11701 ccgtcctcat caccgctgtc ggcgcgagtt tcgcgctcga gtacggcatc gcagttgcct
    11761 tcggcccgac cccggaggtg taccagatcg gcatccacgg tggcacgctg gacctcgcag
    11821 gcgcgcgcat gacctatgcg cagctggcga tgatggcgat cgcggtcgtg ctcatgatcg
    11881 ccctcgacac cctggtccgg aagacccgtt cgggccgtgc gatgcgggca atctcgcagg
    11941 accccaaggc ctgtctgctc atggggatca acgtcgaccg ggtcatctcg cggaccttct
    12001 ttctcgggtc ggcgctggcc ggcgccgcgg gcgtcatggc cggcgcgtac tacgggaaga
    12061 tcgacttcct gatgggtttc atcatcgggc tcaaggcatt caccgcggcg gtcatcggcg
    12121 gcatcgggaa cctgcgcgga gccgttctcg gcgcggtcgt actcggcctg ctggagtcgt
    12181 tcggggtcgc gtacctcggc agcgagtggc gtgacgtctt cgcgttcggt ttcctcatcc
    12241 tcttcctgac cgtgcgcccg acagggctgc tcggcgagcg tgtcacggag cgtgtgtgat
    12301 gaacgacgag tcgaccctgc gcgccggcga ggaggcctcg gcctacgtca acgccgtgga
    12361 ggagcgcggg cctgcgaaca tcgcgctcgc gggcaaggga gtcgggacgc cgaaggcgag
    12421 ccgtgcggcc atcgctcagg cggcgtcggg cgtgctggcg agcaagtggc tcggcctcgg
    12481 cgtggccgtt gccgcggtgg cactgccata cctcaactcc agcacctaca ccatccagct
    12541 cgccacggac gcgctcatct tcgtgatgct cgcggtcggg ctgaacatcg tggtcggcta
    12601 ctgcgggctg ctcgacctcg ggtacgccgc gttcttcgca gtcggcgcgt acacctcggg
    12661 cctcctggcg acaggcttcg gttggccggt gatcgcgacc atccccgtgg tcgtgctcgc
    12721 cgcgatcatc agcggactcc tcatcggcgg tccgacgctt cgactgcgca gcgactacct
    12781 ggccatcgtc accctcggtt tcggggagat catccgcatc acggcgaaca acctcgactt
    12841 cacc
//
