LOCUS       NC_014925              12151 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Staphylococcus pseudintermedius HKU10-03, complete sequence.
ACCESSION   NC_014925
VERSION     NC_014925.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius HKU10-03
  ORGANISM  Staphylococcus pseudintermedius HKU10-03
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2617381)
  AUTHORS   Tse,H., Tsoi,H.W., Leung,S.P., Urquhart,I.J., Lau,S.K., Woo,P.C. and
            Yuen,K.Y.
  TITLE     Complete genome sequence of the veterinary pathogen Staphylococcus
            pseudintermedius strain HKU10-03, isolated in a case of canine
            pyoderma
  JOURNAL   J Bacteriol 193 (7), 1783-1784 (2011)
   PUBMED   21278300
REFERENCE   2  (bases 1 to 2617381)
  AUTHORS   Tse,H., Tsoi,H.W., Leung,S.P., Urquhart,I.J., Lau,S.K.P., Woo,P.C.Y.
            and Yuen,K.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2010) Microbiology, The University of Hong Kong,
            Pokfulam Road, Hong Kong, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000185885.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 09:14:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,448
            Genes (coding)                    :: 2,410
            CDSs (with protein)               :: 2,410
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 14 of 38
            Pseudo Genes (incomplete)         :: 26 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 7 of 38
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:32:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 462900
            Orig. end                         :: 475051
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP002439.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA and bacteria are available from Prof. KY Yuen
            (hkumicro@hkucc.hku.hk) at the Department of Microbiology, The
            University of Hong Kong.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12151
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12151
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12151
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7151](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7151
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            2990..4246
                     /locus_tag="SPSINT_RS02245"
                     /old_locus_tag="SPSINT_0429"
     CDS             2990..4246
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /locus_tag="SPSINT_RS02245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPSINT_0429"
                     /product="glycosyltransferase"
                     /protein_id="WP_015728740.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2e-18, bitscore:
                     64.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRKNKAFLNISCETYNKIKELNKFIESKDWESKRVKNDTELIEK
                     INKLEIISEYTTISVVYIVKNERDYIIKSLLSILDLADEIIIVDTGSEDNTLDLIKKMC
                     DKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVRKNDNLKFYLTYLRIFDDF
                     ENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSLNNSVSYANLNVEVLGIRR
                     GSQKKTNYYNKLLLKTIIDFPKEGRWYYLYLRDNIENMNYNQMLKFCKKIIFKRDAISG
                     IEINNNYYSVRIIFLLMFKLLEESNHEGFIYYMELLEKDFNHTQDFIFLKYSYYIKKLN
                     IDATNLLKSFLENYDLLDEGDVIFSKECTHSLLGAYLFWGGYLKESKKVFEELEVNGKD
                     TVCFYNKKFIYDCLSTLSK"
     gene            4318..4626
                     /locus_tag="SPSINT_RS02250"
     CDS             4318..4626
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.4"
                     /locus_tag="SPSINT_RS02250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3884 family protein"
                     /protein_id="WP_049770161.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRVPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLKKVKFYLDYLPFSITNEIPF"
     misc_feature    complement(4809..4822)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: strong, score: 21.85"
                     /tool="antismash"
     gene            5001..7151
                     /locus_tag="SPSINT_RS02260"
                     /old_locus_tag="SPSINT_0430"
     CDS             5001..7151
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="SPSINT_RS02260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPSINT_0430"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_015728741.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2e-38, bitscore:
                     128.4, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 4.6e-271, bitscore:
                     898.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSLDFLRRKLRTT
                     VNGTTAYGLVQVADKLGFETVPIKADISLFEIDDVQYPFIVHVKKNNNHFHYYVVIRCD
                     KKYIYIADPDPVVNLTKMTYREFKEEWTGVTILLKPTSLYKPIKQDKVRLTSFIPLLLK
                     EKRLIIKIIIISILVNMINILGSYFMKSIIDFYIPQKLKTWVSMISMALIASYIIQQIL
                     SYLQKYFLILLVKTYSINILSPYIKHLFRLPITFFETRRAGDILSRLNDANSITDALAS
                     TMLSLFLDVTIIVGISIFLYYQSTILFVLILIIIPIYAVIVFGFMNSFERKRIKSMEAN
                     ATLSSYLMENITGMETIKALTCEKKCYKQIKYNFSNYYEKTFLLNKLNIKQNVLKKVVQ
                     LILNVSILWVGANLVIDNQISIGTLLVFNSLVIYFMNPIENIMNLQLKLQIAGVANNRL
                     NEIFTVKSEFEKNKLDIKLNQNSSKLIFSNISYGYGYEKEILTDINITIEHNSKVAFIG
                     ESGSGKTTLAKMMVSFFEPSKGKITLGGLDITNINKSVLRQQIIYIPQQTYIFNGTILE
                     NLLLGVVKEVTQKDIDSAIKMAELKSFIDSLPLGLDSVLSSDFSALSGGQRQKIALARA
                     LLTNSSILIMDEATSNIDILTERKIINNLMALNKTIIFISHNLNITQKVDKIFVLAKGK
                     IIEEGSHEFLLKAKGYYSNLIN"
     gene            complement(7408..8223)
                     /locus_tag="SPSINT_RS02265"
                     /old_locus_tag="SPSINT_0431"
     CDS             complement(7408..8223)
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="SPSINT_RS02265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPSINT_0431"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_015728742.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNS
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            8335..10212
                     /locus_tag="SPSINT_RS02270"
                     /old_locus_tag="SPSINT_0432"
     CDS             8335..10212
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166146.1"
                     /locus_tag="SPSINT_RS02270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPSINT_0432"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="WP_015728743.1"
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEVRQQTKQRALFKQELAWMRAGVKARTTK
                     QQARQDRFHDLKEQVQSHQVQGKGSLNLAYSRLGKQVFELENVTKRIDGRTLFENVSTL
                     IQKGVQIGIVGPNGVGKTTMLNILAGEDQHFEGVLKIGQTVKVAYFKQTDERLNRDVRV
                     IDFLREESELARQKDGTTVSITQLLERFLFPSVTHGKKVYKLSGGEQKRLYLLKLLVHQ
                     PNVLLLDEPTNDLDTETLTILEDYIQTFGGTVITVSHDRYFLDKVVDCYWYIHDGQIDM
                     ILGQFEDYLAYQKQLEQAATQVASVKQQPTLQPKKKKGLSYNEKREYDMLLVRIEETES
                     RLAEIDAEMIEANADYAKIKALNEEKETLEAQYDTDMTRWSELEEILEQ"
     gene            10222..12003
                     /gene="recQ"
                     /locus_tag="SPSINT_RS02275"
                     /old_locus_tag="SPSINT_0433"
     CDS             10222..12003
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="SPSINT_RS02275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPSINT_0433"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_015728744.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFEQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDEAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLVEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHRS"
ORIGIN
        1 atcttgtgaa atacttagga ccttacaact ttacagtata tgatggtgtc aaaacagtac
       61 agaacaatca acaaaaggca ctcgcttctg aagatgattt aacagaagta ttgaattata
      121 caaaacaaaa acaagctaca ccgaatatgg aatactatgg aaaagctaaa ggtaaaaatg
      181 tcattaaaat ccatttagaa agcttccaaa cattcctaat taataaaaaa attcatggtg
      241 aggaagtgac gccgttttta aataaattgt cgtcaggtaa tgaggacttt agatattacc
      301 cacatttcta tcaccaaact ggacaaggta aaacgtcaga cgctgagttc acaatggaca
      361 acagtatgta tggtttacct caaggttcgg ccttttcatt aaaaggtgac aatacttttc
      421 aatctttacc agctattatg catcaacaac aaggctatac aacaaatgtc atgcacggtg
      481 actataaaac attttggaac cgtgaccaag tatacagaca ttttggtatc gataagtttt
      541 atgatgcaac atattttgac atgtctccag aaaacttaga aaaccttgga ttaaaagata
      601 aagagttttt caaagaggcg gcggattact tatctaaaga aaaacaaccg ttctattcac
      661 atttaattac gttgacgaac cattatcctt ttacattaag tgatgaggac gcaacgattg
      721 caaaaggtga cacaggtaac tcaacggtcg atggttacat tcaaacagcg cgttacttag
      781 atgagtcgtt agaacagttc attaatgact tgaaagagcg tggcctgtac gatgattcag
      841 tcattatgat ttacggtgac cattatggta tctcagaaaa tcataacaaa gcgatgtcac
      901 agttattagg tgaagagatt acaccagcga agtttaatga cttgaatgca acgggtttct
      961 ggttaaaagc accaggggtt gagcctaaag tggacgacac gtatgcaggt caagttgatg
     1021 tgatgccaac attacttcac ttgttaggta ttgatacgaa aaattatctt atgtttggta
     1081 cagatatgtt atccaaggat cataaagaaa ttgtaccatt ccgtaatggt gatttcgtga
     1141 cgagaaatta taaatatgtc aacggtaaag cctacagtaa caaagacaat caaccgttag
     1201 aaacaccgcc agcagatctt gataaggaca aaaagcaggt acaacatgac ttagaaatga
     1261 gtgataaagt cttgaatgga gacttattcc gattctataa aaatcctgat ttcaacaaat
     1321 tgaacccagc agattataaa tatccgacag gtccaaaggc aaatcaaaaa taaaatgtta
     1381 tgacagggaa gtgtacaaat cagtgcattt ccctgttttt taggttgtat atatttggtt
     1441 gtgtattttt aacggttggt gagagattta ctgcccgttt tttaattttg aaagacctgt
     1501 ttatttaaat ttgaagaagc atcgttttta ctttaaagct tgtcaacaaa cctttataac
     1561 taaaacaccc tatattcaac cccgaagtct caaataaggt caaacgcttg atgacgcgga
     1621 agctaactaa attcacatct gaaaaagatg tcgctaaaag tctatgtgta tcaccttcaa
     1681 ctgttcattg ccatttaaaa gaggtgagta atttgtaaag acatacgcac atgatgcttt
     1741 acctgagcac ttagcttttg acgaattcaa atcaacgaaa gatatcgaag gaccaatgag
     1801 ctttatttac tgtgatagtg taacccatga tatcatcgac attttaccta atcgttgcaa
     1861 acattcatca aatatttgcc ctgaacctca aatactatgc atcatcctca tttaacaaat
     1921 ggcccgattg aaaggattaa taataaaaca aatgtgtgtc ttatagttat agaaatttct
     1981 ggaatcttaa tcatttctaa gatacttgta agtgcatata aaaaacggaa tgggtcttaa
     2041 aaatttttga tgctattgtt gcaatatttt tattaaactt gtccgcatta cgatttgtgg
     2101 ttttaattgt catcatggtt tcgctttcct agagccggcc cttcaactca attcggcttg
     2161 attgaagcca aattggattt taggagcagt acagaaacct catatgcaac aagatttcgt
     2221 tgtactgcct tgcatggctg attagacttc tcaaaagtga aaacactgag aagtcagccc
     2281 gctactgcga taaacgcctc ccaccattaa agtaaagagg cgactgtcaa cagtatgcac
     2341 gttccccctc aggcgtctca gccatctggg taatcctata tcaatacagc gaaactgagt
     2401 gcaagattat gaaatccaga aaacaatggc atcatttttt atacacattc accccttatt
     2461 ttcgaaggat caatcattat taatcgatat cagtctataa atagaagcag agattaggaa
     2521 accataatgt ctcagtgccg ttattgtctc accaaccgac gttgacaaag aacccgtctc
     2581 gatcgcctct atcgttggaa agtattatag attaaaacat ttcctttcat aaaaagaata
     2641 aacttgtcca tatttatctt ggcgtttata taaaaaaagc agcattttca tttcgccatt
     2701 ttcctacatt ttataaccgt catctacact gcatgtactt ctgtaattaa agtatatgtg
     2761 agaaattata cttcctaata taaataaata tggacactat atttaaaaag taatacaatt
     2821 atttataatt aaagcatagc aatttgtaag tgtgatagta gaattacgca tttatcataa
     2881 taattcgcct ctcagtcttg caaaactttt aaaaatattt tgttttatta tttttttttg
     2941 agggggtgtg attaactttg aaaaacatat accctatatt tgatgaagat tggttagaaa
     3001 aaataaggca tttcttaata tttcttgtga aacatacaac aaaataaaag aattaaataa
     3061 atttatagaa tcaaaagatt gggagtccaa aagagttaaa aacgatacag aattgattga
     3121 aaaaataaat aaattagaga ttataagtga atatactaca atttctgtag tgtatattgt
     3181 taaaaacgaa agagattata ttataaaaag ccttttgagt attttagatt tagccgatga
     3241 aatcattatt gttgacactg gttctgaaga caacacttta gatttaatta aaaaaatgtg
     3301 tgataaaaaa ataaaaatat ttacatttaa ttggtgtgat gatttttcaa aagcaagaaa
     3361 ctttgcaaat gctaaagcaa cttgtgactg gattatgatt ttagatgctg acgaaattgt
     3421 tagaaaaaat gataatttaa agttttactt aacgtattta agaattttcg acgactttga
     3481 aaatacggcc tttaatttta aagtaattag agatgaagaa gtatataaaa caagtaagtt
     3541 gataagaaat actaatactt tgacgtacaa aggtagagta cacgaaacat tcttttcttt
     3601 aaataattct gtatcttatg ctaatttaaa tgtagaagtt ttaggaatta gaagaggaag
     3661 tcaaaagaaa acaaattatt ataataaatt gcttcttaaa acaattatag atttcccaaa
     3721 agaaggtcgt tggtattatt tatatttgag agataatata gaaaatatga attataatca
     3781 aatgttaaaa ttttgtaaaa aaattatatt taaaagagat gcgatatctg gaatcgaaat
     3841 aaacaacaat tattactctg ttagaattat tttccttttg atgtttaagt tattagaaga
     3901 atccaatcat gaaggtttta tttactatat ggagttgtta gagaaagatt ttaatcatac
     3961 tcaagacttt atttttttga aatattcata ctatataaag aaacttaata tagatgctac
     4021 aaatttattg aaaagtttct tagagaatta tgatttgtta gatgagggag atgtaatttt
     4081 ttcgaaagaa tgcactcatt cgttattagg agcctattta ttttggggag gatatttaaa
     4141 ggaaagtaaa aaagtatttg aagaattaga agtaaatggt aaagatacag tttgttttta
     4201 taataaaaaa tttatttatg attgtctatc gactttaagc aaataaatat tgatattatt
     4261 atagctcagg aatctaccgc attaaggtaa caggtagtgt gaaggagtga gaaatctatg
     4321 attaaaatat ctttaaagaa aattttatgt caattatctc aaaaaaaact ttatgaaaaa
     4381 acatttcaaa gtatttatat agttgatttt agtcttttag atagggttcc tctatttaaa
     4441 gatgaattta aagtaatagg aacttggtat agctatagtg gtaagcgttg gatatgccat
     4501 acggaattat caaccgagca attcaagaaa atgataacaa aaaatataga ccataaagat
     4561 ttaaaaaaag tcaaatttta tttggattat ctcccatttt ctataactaa cgagattccg
     4621 ttttgaaaat aaatcataca tttaattgat ttttaatagt attattaaat tgttctattt
     4681 aggagggata gatataaaaa gtattgataa atttaaagtg atatcaaatg aggtactgaa
     4741 agacttaagt ggtgggatgt cccgttgtaa atgggctcag attgcatgta taaacgacag
     4801 tatgttggtt agattcggta catctgatcc agagagtgca tgtgattata tgaaaaaaat
     4861 atgtaaagat gaataaccat taaagctttt aataatagga ttgaattttg acatgatatt
     4921 aagtgggtgg gaattaccgc ccgctttttg aatatttaaa tgttgttcta aaaagtggtg
     4981 ggaaggagta gaagtgtcga atgaaatttt caaaacgatt ttatcgtgca caagtagatt
     5041 ctcaagattg tggcgcggca gcgctcgcta tgatattaga attttacggt tctcactatt
     5101 ctttggattt tctgagaaga aagttaagaa caacagtaaa tggcactaca gcatatggtt
     5161 tggttcaggt agcagataag ttaggttttg agacagttcc tattaaagca gatatatctt
     5221 tgtttgaaat tgatgatgta cagtatcctt ttatcgttca tgttaaaaaa aataacaatc
     5281 attttcacta ttatgttgta attagatgtg ataaaaagta tatctatata gctgaccctg
     5341 accctgttgt taatttaaca aagatgactt atagggaatt taaagaagag tggactggag
     5401 ttacaatctt attaaaacct acatctttat ataaacctat aaagcaagat aaagttcgtt
     5461 taacttcttt tataccttta ttattaaaag aaaaaaggtt gattataaaa ataataatta
     5521 tttccatttt agtcaatatg ataaatattt taggttcata ttttatgaag tcaattattg
     5581 atttttatat tccgcaaaag ttaaaaacat gggttagtat gatttccatg gctttaattg
     5641 cttcttacat tatacaacaa attcttagtt atttgcagaa atattttttg attttattag
     5701 taaagacata ttctataaat atactttcac catacattaa acatttgttt agattaccta
     5761 ttactttttt tgaaacgaga agagcaggag acatactatc aagacttaat gatgcaaatt
     5821 caattactga cgctttagct agtaccatgc tatcattgtt tttagatgtt acaataatag
     5881 ttggaatatc tatcttcctg tattatcaaa gtactatttt atttgtatta atattaatta
     5941 tcattcctat ttatgccgta attgtttttg gttttatgaa ctcttttgaa cggaaaagaa
     6001 taaaatctat ggaggcaaat gctactttgt cttcttattt gatggaaaat ataacaggta
     6061 tggagacgat aaaagcactg acatgtgaaa agaaatgcta taagcagata aaatataatt
     6121 tttcgaatta ttacgaaaaa acattcttgt taaacaaact aaatataaag caaaacgttt
     6181 tgaaaaaggt tgttcaatta atattaaatg tatcgatttt atgggtaggc gctaacctag
     6241 ttatagataa tcaaatctca attggaactt tgcttgtttt taattcatta gtgatttact
     6301 ttatgaatcc aatagaaaat attatgaatt tacagcttaa attacaaata gcaggtgtgg
     6361 ctaataatcg attgaatgaa atattcacag ttaaatcaga atttgaaaag aataaattgg
     6421 atatcaaatt gaaccaaaac tcaagtaaac ttatattttc aaatatttca tatggatatg
     6481 ggtacgaaaa agaaatcctt acagatatta atataacaat agaacataat agtaaagtag
     6541 cctttattgg agagtctgga tcaggtaaaa caactttggc gaaaatgatg gttagttttt
     6601 ttgaaccttc aaagggtaaa ataacactag gtggattgga cataacaaat atcaataaat
     6661 ctgttttacg acagcagatt atatacattc cacaacaaac ttatattttt aatggaacta
     6721 tacttgaaaa tcttttatta ggggtagtta aagaggttac gcaaaaggac atagatagtg
     6781 ctattaaaat ggctgaacta aaatccttta ttgatagctt accacttgga cttgatagtg
     6841 tattaagttc tgatttttct gctttgtcag gaggtcagcg gcagaagata gctttagcaa
     6901 gagcactatt aacaaattct tctatattga taatggatga agcaacgagt aatattgata
     6961 tactcacgga gagaaaaatt attaataatt tgatggcgtt aaataaaact attatattta
     7021 tctcccataa tctaaatata actcaaaaag tagataaaat atttgtttta gcaaaaggaa
     7081 aaattataga agagggctca catgaatttt tattaaaagc aaaaggttac tattctaact
     7141 taattaatta ataatggtat tttttgtatt aacacaacca atgtgaagaa cgaaatgaag
     7201 gtactgtttt ttcacacctt ttattttctg aattttacaa ttataactta agttaattat
     7261 tatattttgg tatattggtt gatattttat atagtcaaca gtgtgaaatc tttgttgtga
     7321 gttgttaaag agtattttta atgtatcgat atatgtacac atcaaaaaac cgctcagtgt
     7381 ttcatcacat tgaacggttt tttgaattta ccctaaagca acatctaata tcatcatgat
     7441 ggtaaagcca accatcaagc ttagtgtcgc aagatctgtg tttttaccgg cttgtgaatc
     7501 aggaattaat tcttcaacga cgacaaaaat catcgcccct gcagcaaaag cgagtgcgta
     7561 tggtagtatc ggagtaacga ccaacacagc agctgcgccc actgtagcaa aaacaggttc
     7621 tacaagtgct gaagcttgtc catagttgaa ggctttccat ttggactgtc ctgaagcatg
     7681 tatcggtaat gataatgcag ccccttcagg aatgttttga atcccaatac caattgcaag
     7741 tccgagtgcc cctaacaaag ttgcgtgttc atttcctgtc gcaataccac caaaggcaac
     7801 cccgattgcg agcccttcag gaatgttatg caatgtaatc gcaagtaata aaagtgtgtt
     7861 tttatttaga ctcgtctcaa caccttcttg atgttgtgat tcatcagttg cgtttcgatg
     7921 catgtgtgga ataacatagt ctaaagaacg gatgaatacc ccgccgagta aaaagccaat
     7981 tgcagcaggg agccacggta cagtactgcc cgaactgctt tcaatggccg gttggagtaa
     8041 tgaccaaaaa cttgcggcga tcataatacc agcagcaaag ccttgcatcg aattcaacac
     8101 tttttcgttg acacttttaa atacaaatac ggttgcagcc cctaatgcgg ttaataacca
     8161 cgtgatgaca cctgcaagta atgcttgtaa aaagggcggc atgtttgaaa tataatccaa
     8221 catagaaata gctccttgta tcatttgttc caaaaaaagt gtataatata tagaactgat
     8281 ttaaggttaa cagataaatg cgagtaaatc tataagaaaa agggtgtatg tgttgtggaa
     8341 gcgtataaaa ttgaacgcct caacaaatct tatgcggata aagtcatttt tgatgattta
     8401 cagttatcca tttccaatca agagcgaatc ggattagttg gtattaatgg aacggggaaa
     8461 agttcattgc tcaaagtcat cgcaggcatg gatgaagatt ttgatgccgt tcaatcgcat
     8521 ccgaaacaat atcgtattcg ttatgcggca caaaatcata atttggagcc aacgttaacg
     8581 gtttatgaag cggtttatag tgcagattcc aaagagttgc aagtcattaa acgttatgaa
     8641 gaagctactg cacaatacgc agagcaacag actgacaaag cgtttgatgc aatgatggca
     8701 gcacaagctg atatggatgc ttacagtgct tgggattata gtgctgaaat taaaacgatt
     8761 ttatcgaaac ttggcatcca cgatacgaca cgccgaatga gtgaactctc aggtggccaa
     8821 caaaagcgtg tagtgttagc gcgtacgttg attgaaaaac cagatttatt attattagac
     8881 gaaccgacga accatttaga tttcgaatcg attcattggt taattcaatt tgtaaagtct
     8941 tatccgaaaa cagtcgtttt tgtcacgcat gaccgttact ttttaaatga agtggcgacg
     9001 cgtattgtag aattgcgaaa tggcaagcta gatacatatc caggtaacta tgaagcgtat
     9061 attgcaatgc gagcagagcg ggaagaaatc gaagtgcgtc aacaaacgaa acaacgtgca
     9121 ctttttaaac aagagttagc gtggatgcgt gccggtgtca aagcgcgaac tacgaagcaa
     9181 caagcaaggc aagatcgttt tcatgacttg aaagaacaag tacaatcgca tcaagtgcaa
     9241 gggaaggggt cattgaatct ggcttattcg cgtttaggca aacaagtctt tgaacttgaa
     9301 aatgtgacga aacgtattga tggccgtaca ttatttgaaa acgtgtcgac attgattcaa
     9361 aaaggtgttc aaatcggtat tgtgggccca aacggtgtcg gcaaaacgac gatgctgaac
     9421 attttagcag gtgaagatca gcattttgaa ggtgtattaa agataggtca aacggtaaaa
     9481 gtggcatatt ttaaacaaac ggatgaacga ttgaatcgtg atgtccgtgt cattgatttc
     9541 ttgcgtgaag aaagtgagtt ggcgcgtcaa aaagatggga cgaccgtttc gattacgcaa
     9601 ttgttggaac gctttttatt ccccagtgtg acgcatggta agaaagtgta taaactctca
     9661 ggtggcgaac aaaagcgttt gtatttattg aagttgttag tgcatcagcc gaacgtgttg
     9721 ttgttagacg aaccgacaaa tgatttggat acagaaacgt taacgatttt agaagactac
     9781 attcaaacat ttggcggtac cgtcattact gtgagccatg accgttactt tttagataaa
     9841 gtcgtggatt gttattggta tattcacgac ggtcaaattg acatgatttt agggcagttt
     9901 gaagattatt tagcatacca aaaacaattg gaacaagcag caactcaagt agcgtccgtg
     9961 aaacaacaac cgacattaca accaaaaaag aaaaaaggat tatcatacaa cgaaaaacga
    10021 gaatatgaca tgttattagt gcgcatcgag gaaacagagt cgcgtctggc tgaaattgac
    10081 gccgaaatga tcgaagcgaa cgcagattat gccaaaatca aagcattaaa tgaagaaaaa
    10141 gaaacgctcg aagctcaata tgataccgac atgacgcggt ggagtgaact agaagaaata
    10201 ttagaacaat agagggatag catggagcaa acattgtcgc attattttgg atacaaatct
    10261 tttcgtcctg gtcaaaaaga aattattgaa cgcgtattga atcatcatca tacgttaggg
    10321 gtgttaccca caggtggggg taaatcgtta tgttatcaag taccgggatt gatgtttaaa
    10381 ggaacgacca ttgtcattag tcctttaatt tcgttaatga aagaccaagt ggaccaattg
    10441 accgcaatgg gaatatctgc ggcttatttg aatagtagtt taagtacgca agcacaaaaa
    10501 gagatagaaa cgcaattggt caatggtgag ttgaaatttt tatacatcgc accggaacgt
    10561 tttgagcaaa cttattttga aatgttgttg cgacgtgtcg atatttcact cgtcgcattt
    10621 gacgaagcac actgtatttc aaaatgggga catgattttc ggccgagtta tcaagcagtc
    10681 atacaacgcg tattgaccat gccgcaacag ttttcgattg tcgcattgac tgcaacagcg
    10741 acgactgaag tgcaacaaga tattatggaa cggttaatga tttcgcgaca acacttagtc
    10801 aaaactagta ttcgtcgacg taatttgaag ttccaggtga atgggacgta tcaacgtcaa
    10861 aagtttgtat tggattatat tcaaaaacat agtcagaaag cggggattgt ttattgttca
    10921 acgcgtaaac aagtagaagc cttgtatgaa gctttagaag acgcagatat ccctgtatcc
    10981 tactatcatg caggaatgac agcgaaacaa agagatgaag cccagaatga ctttttatat
    11041 gaccgtactc gtgtagtcgt tgcgaccaac gcattcggta tgggaattga taaatcgaat
    11101 gtccgttatg tcattcatta caatatgccg ggtgatttgg aatcgtatta tcaagaagca
    11161 ggacgtgcag gtcgagacgg attagatagt gattgtattt tattgtacag tgatcgagat
    11221 attgctttac atcagtattt tatcggtgcc tcgaaagcgg acgatgatta caaggaacgt
    11281 atgggcgaaa aattgacgaa aatgattcaa tatacgaaaa cgacgaaatg tctagaagca
    11341 acattagtcc attattttga gccgaatgaa aaacttgaag aatgtcacca atgtagtaat
    11401 tgtattgata aaaataaaac gtacaatatg acgaaagaag cgaaaatgat cattagcggt
    11461 gtcgcacgtt tacgtcaaca agaaggatat caaacggtca ttcaagtttt gcgtggcgag
    11521 gtttcggact acattcgtca tcaaggctat gaaaatcttt cgacgtatgg tttgatgaaa
    11581 gattatacga ctggcgagtt acatcattta atcgatgaac tccgctttaa aggctttctc
    11641 aacgaacatg acgaaatact catgtgtgat gcctcagtag aaaagttatt gagtgaggat
    11701 acgcaagtgt ttacaaggcc gttcaagcgt aagtcgaaag aaacagtgca tattaacact
    11761 gtagaagggg tcgaccgcac gttatttgaa aagcttgtcg aagtcagacg tgaaatgagc
    11821 gaacgattaa acatgccacc gacgaatatt tttacagact atacgttaga agcttttgcg
    11881 aaaagaaagc ctgtcacaaa gcaagaaatg attcagattg atggtgttgg tagttataag
    11941 ctgaagcact attgtcctga atttttagaa gcgattcaag aatataaaat gcatcgttcg
    12001 taaaacatca gatggagctg ggggaattcc tggctccttt tttacaaata aacgatttct
    12061 tattgagtgg taaagaatgg aaaagtgaga cgtgtcgata gtagataatt taaatttttt
    12121 agatgcaaat tgtgttattt ttacaatgaa t
//
