LOCUS       NC_015953              11311 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. SirexAA-E, complete sequence.
ACCESSION   NC_015953
VERSION     NC_015953.1
KEYWORDS    GSC:MIGS:2.1; RefSeq.
SOURCE      Streptomyces sp. SirexAA-E
  ORGANISM  Streptomyces sp. SirexAA-E
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7414440)
  AUTHORS   Takasuka,T.E., Acheson,J.F., Bianchetti,C.M., Prom,B.M.,
            Bergeman,L.F., Book,A.J., Currie,C.R. and Fox,B.G.
  TITLE     Biochemical properties and atomic resolution structure of a
            proteolytically processed beta-mannanase from cellulolytic
            Streptomyces sp. SirexAA-E
  JOURNAL   PLoS One 9 (4), e94166 (2014)
   PUBMED   24710170
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414440)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Ovchinnikova,G., Davenport,K., Detter,J.C., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Adams,A., Raffa,K., Adams,S., Book,A., Currie,C. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Streptomyces sp. SirexAA-E
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7414440)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Ovchinnikova,G., Davenport,K., Detter,J.C., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Adams,A., Raffa,K., Adams,S., Book,A., Currie,C. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2011) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. SirexAA-E
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            environment                       :: Soil, Terrestrial
            num_replicons                     :: 1
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Free living
            trophic_level                     :: Missing
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: Missing
            sequencing_meth                   :: WGS
            assembly                          :: Newbler v. 2.3 (pre-release)
            finishing_strategy                :: Finished
            GOLD Stamp ID                     :: Gi05895
            Funding Program                   :: DOE-GLBRC 2008
            Source of Isolate :: Cameron Currie (currie@bact.wisc.edu)
            Cell Shape                        :: Filament-shaped
            Motility                          :: Nonmotile
            Sporulation                       :: Sporulating
            Temperature Range                 :: Mesophile
            Gram Staining                     :: Gram+
            Diseases                          :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Finished
            Current Finishing Status          :: Finished
            Assembly Method                   :: Newbler v. 2.3
            Genome Coverage                   :: 30x
            Sequencing Technology             :: 454/Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000177195.2-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 02:12:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,619
            CDSs (total)                      :: 6,532
            Genes (coding)                    :: 6,385
            CDSs (with protein)               :: 6,385
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 61 of 147
            Pseudo Genes (incomplete)         :: 120 of 147
            Pseudo Genes (internal stop)      :: 8 of 147
            Pseudo Genes (multiple problems)  :: 39 of 147
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:50:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5953055
            Orig. end                         :: 5964366
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP002993.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4086644
            Source DNA and organism available from Cameron Currie
            (currie@bact.wisc.edu)
            Contacts: Cameron Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11311
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11311
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11311
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6311](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6311)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            663..2126
                     /gene="hemG"
                     /locus_tag="SACTE_RS26160"
                     /old_locus_tag="SACTE_5262"
     CDS             663..2126
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579252.1"
                     /locus_tag="SACTE_RS26160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5262"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_014049028.1"
                     /sec_met_domain="DAO (E-value: 8e-07, bitscore: 27.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRSLQHDPARTGHVVVIGGGIAGLAAAHRLLATGLRVTVLEATE
                     RLGGKLMTGEVAGVRVDLGAESMLARRPEAVELATAAGLGDRLQPPATASASVWTGNAL
                     RPMPKGHVMGVPGDPAALSGLLSSEGLARIAEERDLTPTAVGEDVGVGAYVADRLGREV
                     VDRLVEPLLGGVYAGDAYRISMRAAVPQLFEVAREGGSLLDGVRRIQERAAVRQPTGPV
                     FQGISGGLGTLPGAVADAVRAGGTEIRTATPVLGLTRTGQGWEVRTDSGVIAADGVVMA
                     APAWSASTLLAAECPAASVELAGVEYASMALVTLAFRRSDVEGNEALAGRSGFLVPPVD
                     GRTIKASTFSSNKWRWVADAAPDLFVLRTSVGRYGEEDHLHREDSELVAVSLKDLADAT
                     GLTARPVDTVVTRWIGGLPQYPVGHLGRVARIREEVAKLPGLRVCGAVYDGVGIPACVA
                     GAHRAADEIAEEIIATSTRVQGTPSEAGQ"
     gene            2131..2844
                     /gene="hemQ"
                     /locus_tag="SACTE_RS26165"
                     /old_locus_tag="SACTE_5263"
     CDS             2131..2844
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888541.1"
                     /locus_tag="SACTE_RS26165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5263"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_014049029.1"
                     /transl_table=11
                     /translation="MSAPEKLPNAGKKAKDLNEVIRYTLWSVFKLRDVLPADRAGYADE
                     VQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETSDALQEAYNLFRRTTLGRALD
                     PVWSNMALHRPAEFNKSHVPAFLADEEPRAYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGFPDVRANTVPSFSLGDYEWILAFEADELYRIVDLMRHLRGSEARMHVREEVPFY
                     TGRRKAVADLVAGLA"
     gene            complement(3089..3895)
                     /locus_tag="SACTE_RS26170"
                     /old_locus_tag="SACTE_5264"
     CDS             complement(3089..3895)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049030.1"
                     /locus_tag="SACTE_RS26170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5264"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_014049030.1"
                     /transl_table=11
                     /translation="MLWVFFLLAAWGVAAVSCIRLCLLTAGAALLPADAPGKPLSRELS
                     LYETAFLAGGPHRVADLALVSMHLRRRLLLAHTGWATVVDPEGRDEVERTVIRAIGPEG
                     QSRIAPIRASAAAADTVRALADRLVAAGLAVPHGTRTALESAVRGVRGAAVLVVVLAAV
                     TALMPGQDTGDAGPVLAWFGLPLVLTLGCLAIARVENQPYSPWASPSGQRWLDSLPAPV
                     HGPDRDVLAAVAVRGVAAVGDPRLRAALLDGGRTVRPLGRASGAEI"
     misc_feature    3885..3905
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.26"
                     /tool="antismash"
     misc_feature    complement(3886..3906)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    3928..3943
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.2"
                     /tool="antismash"
     gene            complement(3997..5004)
                     /locus_tag="SACTE_RS26175"
                     /old_locus_tag="SACTE_5265"
     CDS             complement(3997..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153333.1"
                     /locus_tag="SACTE_RS26175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5265"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_014049031.1"
                     /transl_table=11
                     /translation="MNALALLLTLAVAVSSVLLLTVPAAGRRRAPGEPEPSVHDLSEAA
                     FLGGGPARVVDTALTALYEDGRLVVGGPGIVAVRRDEARDPVERAVLQELAAAPSGALH
                     VLRKAVMRHPAVQEIGDGLAARGLLVPPAENRTRRRWALVQGIGCVVAAPFAVVLTVLQ
                     YALREGYADVPIPFVVKVLPALLAGAVVGLVTAKSAKARLTEAGRRAATRYRWTRTHVP
                     GAAHLVATQGLGALPHTELRDQLLAAARHPSPGRSVPAGAGATADLYAGDAAWCAGAGP
                     GGGGCGGSGDSGTGSSCGSGGGGSSCGGGSSCSSGSSCGGGSSCGGGSSCGSSS"
     gene            complement(5001..6311)
                     /locus_tag="SACTE_RS26180"
                     /old_locus_tag="SACTE_5266"
     CDS             complement(5001..6311)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049032.1"
                     /locus_tag="SACTE_RS26180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5266"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_014049032.1"
                     /sec_met_domain="DUF692 (E-value: 3.7e-90, bitscore: 300.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLGIGIGWRPEIADALEGLPGIDWVEAVAENICEDHLPGPLARL
                     RERGVTVVPHGVSLGLGGADRPDPDRLAGLAARATLLGAPLVTEHVAFVRAGGPLTASP
                     GLEAGHLLPVPRTWDALDVLCENVRIAQDSLPVPLALENIAALIAWPDEEMTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNRGEDPAAALDELPVEAIAYVHVAGGIEKDGVWHDTHA
                     HPVTRPVLDVLTELRSRVDPPGVLLERDDAFPPAGELAGELTAIRATLAAAAAAPAPDR
                     TPAGATARPAPAPGARERVAMAQTTLLSALVAGSPAPEGFDHARLAVQSRALAAKRADV
                     VAEVAPELPRILGDGFRKAFLAYARTRPLSGGYRRDALDFAEHLLIAGRPADEAARRRL
                     TRWWQDRAAPRPPRRGARVARAARTVLAGARPGRGSR"
     misc_feature    6573..6586
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: strong, score: 24.25"
                     /tool="antismash"
     gene            6913..8265
                     /locus_tag="SACTE_RS26185"
                     /old_locus_tag="SACTE_5267"
     CDS             6913..8265
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049033.1"
                     /locus_tag="SACTE_RS26185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5267"
                     /product="family 43 glycosylhydrolase"
                     /protein_id="WP_014049033.1"
                     /transl_table=11
                     /translation="MTGTENRSHLDRRSLLTRAAGVGAAAALPTAAAGATPASASPASP
                     ASPARRHPSNWPDPEPYGIADIRPDLWPREDNSFVLPLELRPRDKERGLVWMRDTYVNR
                     FVVDGRPLYVATGTTRVPGLEAAGPWNDGIFVWLSRSLKGPWKLADTTRIRPGAEKGKV
                     WSPEFVGENRPGRTVVAPWQEYWYDEQFGKRGQAWAPELHYFRGKWYMVACMGDHSKKV
                     GSFMLVSEGGVEGPYRLVEGNVDKPFGDSFIGGPAFIEPGAYHHIDGSLYSEGDRAWLV
                     LHNNLYARFRDDMEDIVTTTDLPLFKQTPYAPEPYLEGAYVFKHGGKYYLLHAAWDRTS
                     INADGSTRQAYDTAGTGRVQYQYDAVVAVSDRFEGPYSRRWTAGVGAGHNNFFTDSDGT
                     VWATFFRNPAFGHWSDPSRVADAAVPGVVRVEWTGPQGNRLYVRRRDGGRG"
     gene            complement(8270..9043)
                     /locus_tag="SACTE_RS26190"
                     /old_locus_tag="SACTE_5268"
     CDS             complement(8270..9043)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514518.1"
                     /locus_tag="SACTE_RS26190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5268"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_019993147.1"
                     /transl_table=11
                     /translation="MSSDDVSRPLSAPPADSPFRGEPTARDEAPQYVLPLVVHLEKTDP
                     PGRTDAVRTAARAVLTMLSDERSLGDGEWAQAVRDWEDARIRKVVRRARGAEWRKASAL
                     PGITVTGESAEVRVFPPIPLDGWPKELAKLQVSGTELDDPVPPPAPDGTGPVLWLNPGP
                     DMSAGKTMAQAGHGAQLAWWELSDAEREEWREAGFPLSVAVATPESWRELTASGLPVVV
                     DAGFTEIAPGATVVTEGGSRFCPLPRGRRPRGASA"
     gene            9115..9792
                     /locus_tag="SACTE_RS26195"
                     /old_locus_tag="SACTE_5269"
     CDS             9115..9792
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="SACTE_RS26195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5269"
                     /product="AIM24 family protein"
                     /protein_id="WP_014049035.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQQATAPGMTLQNAKSIKYAVDGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLPLMAVRGQGEAWFAHEAANCFIVDMEQGDVLT
                     INGRNVLCFDATLAYEIKTVKGAGMTGGGLFNSVFTGQGKLGLMCDGHPIVIPVSARQP
                     VYVDTDAVVGWSAQLSTSLHRSQSFGSMVRGGSGEAVQLMLQGEGFVIVRPSEVKQEKA
                     SAN"
     gene            10013..10192
                     /locus_tag="SACTE_RS26200"
                     /old_locus_tag="SACTE_5270"
     CDS             10013..10192
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="SACTE_RS26200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5270"
                     /product="hypothetical protein"
                     /protein_id="WP_014049036.1"
                     /transl_table=11
                     /translation="MDDAYCETPAPAPVPEDTGGPYAECVLCREPTEYPESTKGATLCP
                     VCAWQEAGRTACSG"
     gene            complement(10208..11074)
                     /locus_tag="SACTE_RS26205"
                     /old_locus_tag="SACTE_5271"
     CDS             complement(10208..11074)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153326.1"
                     /locus_tag="SACTE_RS26205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_5271"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_014049037.1"
                     /transl_table=11
                     /translation="MTALVILGGMALTGCGGPGAARTETLVPPASPSPVAKAAPERVEK
                     PPTMAPGPAGLTPVFERRAPGGSGSATGSPGRDEKVVALTFDADMTADQGPRAEAGEHF
                     DNPELIALLRRLKVPATVFMTGRWAEEYPSQARSIGTDPLFEIANHSYSHYAFSSPCYG
                     LPTVEEGAMRGEVEQAFDAIRKTGARQVVPYFRFPGGCYDDASRKALAPTGVTAVQWDV
                     VSGDAFATDADAVAQQVLDGVGPGSLVVMHCTRSAAPVTDEAVRQVVPALRERGYRFVK
                     VSELMRG"
ORIGIN
        1 ctctcgacgt cgacgacctg ccccgtgacg tccttggcgt ccgacgaacg cgacagaagc
       61 ctgcccgacc ccgccagctc ctccagcacg ccctcgtagc ggtcctgcgg cacgcgcagc
      121 accaggtgcg agacctcgtg ggtgtcgtcc agccgctccg tggtctcctc ggcgaccagt
      181 ccgccggcgc cttccgcggc cctgcgggcg gcggcgaccg ccttgggcac actcgccacc
      241 tcgacgtcga gcgtggccgt acggatgacg tgggcggcga ccgcgggggc ctgcttcgcc
      301 ttcgacgcgg ccccgtcggc ggaccgctca cccgccccct ccgcgcccgg tgccgccgcc
      361 ttcccgtccg cggacgtggc ccccttgtcg gcggcggagc cgcctccgcc cccggccgag
      421 cacccgccgg cgccgagcag cgccacgagc agggcggcgg ccggcacggc gcgtgccagg
      481 cgcctgccgg accgtgcggg tggatgggtg tccgtgcggt gtccgtcgtg catgtcgcgt
      541 cccccccaag ggccgttcgc cgatgatgcc ggttcgacgt acgggccggt gcgcgggttg
      601 ccggacggcg gtgtcgaagc ggtcacgttc gggactcgtt cggggtttga gagagtggac
      661 ccatgcagcg ttctcttcag cacgaccccg cgcgtacggg gcatgtcgtc gtcatcggcg
      721 gcggcatcgc cggactcgcc gccgcccacc ggctgctcgc caccggactc cgggtcaccg
      781 tcctggaggc gaccgagcgg ctcggcggca agctgatgac cggcgaggtc gccggcgtac
      841 gggtggacct gggggccgag tcgatgctcg cgcggcgccc cgaggcggtc gagctggcca
      901 ccgccgccgg tctcggcgac cggctccagc cgcccgccac cgcctccgcc tcggtctgga
      961 ccgggaacgc cctgcgcccc atgcccaagg gccacgtcat gggcgtcccg ggggatccgg
     1021 cggcgctcag cgggctgctc tcctccgagg ggctcgccag gatcgccgag gaacgcgacc
     1081 tcacccccac cgccgtcggt gaggacgtcg gggtcggtgc ctatgtcgcc gaccggctcg
     1141 gccgcgaggt cgtcgaccgg ctcgtggagc cgttgctcgg cggggtgtac gcgggcgacg
     1201 cctaccggat ctcgatgcgg gccgccgttc cgcagctctt cgaggtcgcc cgggagggag
     1261 gctcgctcct cgacggcgtc cggcggatcc aggagcgggc cgccgtcagg cagccgaccg
     1321 gacccgtctt ccagggcatc tccggggggc tcggtacgct gccgggcgcc gtcgccgacg
     1381 ccgtgcgggc gggcgggacc gagatccgta cggccacccc cgtcctcggc ctgacccgga
     1441 ccgggcaggg ctgggaggtg cgcaccgaca gcggggtgat cgccgccgac ggtgtcgtga
     1501 tggccgcgcc ggcctggtcc gcgtccaccc tgctcgccgc cgagtgcccg gcggcctccg
     1561 tcgaactggc cggcgtggag tacgcctcga tggccctggt caccctcgcc ttccggcgct
     1621 cggacgtcga gggcaacgag gcgctggcgg ggcgctccgg ctttctcgta ccgcccgtcg
     1681 acggacgcac gatcaaggcg tccaccttct ccagcaacaa gtggcggtgg gtcgccgacg
     1741 cggcccccga cctcttcgtg ctgcggacct cggtcgggcg ctacggcgag gaggaccacc
     1801 tgcaccgaga ggactccgag ctcgtcgcgg tctcgctgaa ggacctggcc gacgcgaccg
     1861 ggctgacggc gcggcccgtg gacaccgtcg tcacccggtg gatcggcggg ctgccgcagt
     1921 accccgtcgg tcatctgggc cgggtcgccc ggatccgcga ggaggtggcg aagctgccgg
     1981 gactgcgggt gtgcggcgcg gtctacgacg gggtgggcat ccccgcctgc gtggccggcg
     2041 cccaccgggc cgcggacgag atcgcggaag agatcatcgc cacgtcgacc cgggttcagg
     2101 gcactccgag cgaggcggga caatagccgt atgagtgcgc cagaaaagct tccgaacgca
     2161 ggcaagaagg ccaaggacct caacgaggtc atccgctaca cgctgtggtc cgtcttcaag
     2221 ctgcgagatg tgcttcccgc cgaccgggcc ggctacgccg acgaggtcca ggagctgttc
     2281 gaccagctcg cggccaagga cgtcaccgtc cgcggcacct acgacgtctc cggtctgcgg
     2341 gccgacgccg atctgatgat ctggtggcac gccgagacct ccgacgcgct ccaggaggcg
     2401 tacaacctct tccggcgtac cacgctcggc cgcgcgctgg atccggtctg gtcgaacatg
     2461 gccctgcacc ggcccgccga gttcaacaag tcgcacgtgc cggcgttcct cgccgacgag
     2521 gagccgcgcg cctacgtcag cgtgtacccc ttcgtgcgca gctacgactg gtacctgctg
     2581 ccggacgagg accgtcgccg tatgctcgcg gaccacggca agatggcccg tggcttcccg
     2641 gacgtgcgcg ccaacacggt cccctcgttc tcgctcggcg actacgagtg gatcctggcc
     2701 ttcgaggcgg acgagctgta ccgcatcgtc gacctcatgc gtcatctgcg aggctccgag
     2761 gcgcggatgc acgtgcgcga agaggtcccg ttctacacgg gccgcaggaa ggccgtcgca
     2821 gacttggtgg cggggctcgc atagccagcg tttcccggcc accccgaccc ggaagatcag
     2881 ttggcgggca acggcgcgtg tgacgccgcc cgtcgttcca gcgacaccgg gtccggttcc
     2941 gggagcacat cggccctgac cggcgaagtg cggtggcccg cgggggagtc agccattcct
     3001 ccccgcgggt cgccgcctcg tgccggacgt ggccgatgtg tcccgggccg cacaaccgac
     3061 gggtacgtcc cggggcgcct cgcagatctc agatctcggc gccggacgcc cggccgagcg
     3121 ggcggaccgt acggccgccg tcgagcagcg ccgcccgcag ccgcgggtca cccaccgccg
     3181 ccaccccccg cacggccacc gccgccagca cgtcgcggtc cgggccgtgg accggggccg
     3241 gcagcgagtc cagccacctc tgcccggacg gcgaggccca cggactgtac ggctggttct
     3301 ccacccgcgc gatggccagg cagcccagcg tgagcaccag cggcagcccg aaccacgcca
     3361 ggaccgggcc cgcgtcgccc gtgtcctgac cgggcatcag cgcggtcacc gccgccagca
     3421 ccaccaccag caccgccgcg ccgcgcaccc cccgcaccgc cgactccagg gcggtccgcg
     3481 tgccgtgcgg tacggcgagc cccgccgcca ccaggcggtc ggcgagagcg cgtacggtgt
     3541 cggcggcggc agcggacgcc cgtatcggcg ctatccggga ctgcccctcc gggccgatgg
     3601 cccggatcac ggtgcgctcg acctcgtcgc ggccctccgg gtcgacgacc gtggcccagc
     3661 ctgtgtgggc cagcagcaga cggcgccgca gatgcatcga gaccagggcc agatcggcga
     3721 cccggtgcgg accgcctgcc aggaacgccg tctcgtacag gctgagttcg cgcgacagcg
     3781 gcttaccagg ggcgtcggcc gggagcagcg cggcgcccgc ggtgaggagg cagagccgga
     3841 tacaggagac ggccgccacg ccccacgcgg ccaggaggaa gaagacccag agcatgggcg
     3901 atgtctatgc gaggcgggca cgcgagcgcc acggtctgtt cacgattcgg acagaagtcg
     3961 gtcacagctg gtcacggctg gctgaaatcg tttggttcag gagctgctgc cacagctgga
     4021 cccgccgccg cagctcgacc cgccgccgca gctggaccca ctgctgcagc tggagccgcc
     4081 gccgcagctc gaaccgccac ctcccgaacc gcacgaggac cccgttcccg agtcgccgct
     4141 cccgccgcag ccacccccgc cgggccccgc cccggcgcac cacgcggcgt cgcccgcgta
     4201 caggtccgcg gtcgcgcccg cgcccgccgg gacggagcgc ccgggggaag ggtgccgggc
     4261 ggcggcgagc agctggtccc gcagctccgt gtgcggcagt gcgcccagcc cctgggtcgc
     4321 caccaggtgg gcggcaccgg gcacatgggt gcgggtccac cggtagcggg tggcggcccg
     4381 gcgtcccgcc tcggtgagcc gggccttcgc ggatttggcg gtcaccagac cgacgacggc
     4441 accggcgagg agcgccggca gcaccttgac gacgaacggg atcgggacgt ccgcgtaccc
     4501 ctcccgaagg gcgtactgga gcacggtcag gacgacggcg aacggcgcgg ccacgacgca
     4561 cccgatgccc tggacgagcg cccaccggcg gcgtgtgcgg ttctcggccg gtggcaccag
     4621 cagtccccgg gcggccagcc cgtcaccgat ctcctgcacc gcagggtgcc gcatcacggc
     4681 cttccgcagg acgtgcaggg cgccgctcgg cgcggcggcc agctcctgga gcacggcgcg
     4741 ctccaccggg tcacgggcct cgtcccgtcg tacggcgacg atgccggggc cgccgaccac
     4801 caggcggccg tcctcgtaca gggcggtgag cgcggtgtcg accaccctgg ccgggccgcc
     4861 gccgaggaag gccgcctccg acaggtcgtg gaccgacggt tcgggttcgc cgggggcacg
     4921 gcgccttccg gccgccggca cggtgagcag cagcacggag gagaccgcca cggcgagggt
     4981 cagcagcagg gcgagggcgt tcaccggctt ccccttcccg ggcgggctcc ggcgaggacg
     5041 gtgcgcgccg cccgggcgac ccgtgcgccg cggcgcggtg ggcgcggtgc cgcccggtcc
     5101 tgccaccacc gggtcagcct ccgccgggcc gcctcgtcgg ccgggcgccc ggcgatgagc
     5161 aggtgctccg cgaagtccag ggcgtcgcgg cggtaacccc cggacagggg gcgggtcctg
     5221 gcgtacgcga ggaacgcctt ccggaagccg tcaccgagga tccggggaag ctccggggcc
     5281 acctcggcga cgacgtcggc ccgcttcgcg gccagggcgc gactctgcac cgccagccgc
     5341 gcgtggtcga agccctccgg cgcgggcgag cccgccacca gggcagacag cagcgtggtc
     5401 tgcgccatcg ccacccgctc ccgggcgccc ggcgcgggtg ccggacgggc ggtggccccg
     5461 gccggcgtcc ggtcaggggc cggggcggcc gccgccgcgg ccaaggtggc ccgtatcgcg
     5521 gtcagttcgc ccgccagctc cccggccggc gggaaggcgt cgtcgcgttc cagcagcacc
     5581 cccggcgggt ccacccgcga acgcagttcc gtcagcacgt ccagcacggg gcgcgtcacc
     5641 gggtgggcgt gggtgtcgtg ccagacgccg tccttctcga tgccgcccgc cacatggacg
     5701 tacgcgatgg cctccaccgg cagctcgtcc agtgcggccg cggggtcctc gccccggttg
     5761 acgtggttgg tgtgcaggtt ggccacgtcg atcaggaggc gtacgccggt gcgctcgacc
     5821 agctccgcca ggaactgccc ctcggtcatc tcctcgtccg gccaggcgat cagcgcggcg
     5881 atgttctcca gggcgagcgg cacgggcagc gagtcctggg cgatgcggac gttctcgcac
     5941 agcacgtcca gcgcgtccca ggtccgcggg acgggcagca gatggcccgc ctccaggccc
     6001 ggcgacgcgg tgagcggccc cccggcccgg acgaaggcga cgtgctcggt caccagcggt
     6061 gcgcccagca gtgtggcacg cgcggccagg ccggcgaggc ggtccgggtc gggccggtcc
     6121 gccccgccca ggcccagcga gacgccgtgc gggacgacgg tgaccccgcg ttcccggagc
     6181 cgtgcgaggg ggcccgggag gtggtcctcg cagatgttct ccgcgacggc ctcgacccag
     6241 tcgatccccg gcagcccctc gagggcgtcc gcgatctccg gccgccagcc gatgccgatt
     6301 cccagcttca tggtgtcccc tccccgcctc gtgcaggggt catggccccg cggcgcggcg
     6361 ccgaatccga gaagggggac gttcagagct ggatctgagg ttcccggtgc cggggccttc
     6421 cgcgcgcagg accgtccgcg acctggacga aaacctcggc gttatctgat cgtgatgttc
     6481 cggccggtcg gtcatcacct cgcccacctg caaaaacacc gcgcacacgg cctcccggag
     6541 ggccgggagc gccggattaa atcggcggtc tgttgacgga gatcaacagg cgttcgcatg
     6601 ctgatcgaca tgtcaaccag ggacggctcc acggcacatt ccccgcgggg cgagacgtgc
     6661 gcgcccgccg accgccgctc gcggaaggcg tgtccgggcg gggccgaggc ggccccggcg
     6721 gccgtccctg tcccccgctg acaggcggac cgacgccccg gcccggccgc gacgcggtcg
     6781 cgggcagacg tacggccccg ccgggcgcgg ggcatcccgt ggagagccgg cccgactcga
     6841 tccgacccgg gcccgggcgg gcggtcctgc ccacgcaccg gcaacccgga cgaacgaagg
     6901 agaaactgac tgatgaccgg caccgagaac aggtcgcacc tcgaccggcg gtccctgctg
     6961 acccgagccg ccggtgtggg agcggcggcg gcactgccca cggccgccgc cggggcgacc
     7021 ccggcgtcgg cctcgcccgc gtccccggcc tcgcccgccc gccgtcaccc ctccaactgg
     7081 cccgaccccg agccctacgg catcgcggac atccggccgg acctgtggcc acgcgaggac
     7141 aactccttcg tcctgccgct ggagttgcgc ccccgagaca aggaacgcgg cctggtgtgg
     7201 atgcgggaca cctacgtcaa ccgcttcgtc gtcgacggcc gtccgctcta cgtcgcgacc
     7261 ggaaccaccc gcgtacccgg gctggaggcg gccggaccgt ggaacgacgg catcttcgtc
     7321 tggctgtccc gctccctcaa gggcccctgg aagctggccg acacgacccg catccggccc
     7381 ggcgcggaga agggcaaggt gtggtcgccc gagttcgtcg gcgagaaccg gcccggccgc
     7441 acggtcgtcg ccccctggca ggagtactgg tacgacgagc agttcggcaa gcgcggccag
     7501 gcgtgggccc cggagctgca ctacttccgg ggcaagtggt acatggtcgc gtgcatgggt
     7561 gaccactcga agaaggtcgg ctccttcatg ctcgtgagcg agggcggggt cgagggcccc
     7621 taccggctcg tcgaggggaa cgtcgacaag cccttcggcg actcgttcat cgggggtccg
     7681 gccttcatcg agcccggcgc ctaccaccac atcgacggga gcctctactc cgagggcgac
     7741 cgcgcctggc tcgtgctcca caacaacctg tacgcccggt tccgcgacga catggaggac
     7801 atcgtcacga cgacggacct cccgctgttc aagcagacgc cctacgcacc cgagccgtac
     7861 ctcgaaggcg cctacgtctt caagcacggc ggcaagtact acctcctgca cgccgcgtgg
     7921 gaccgtacgt cgatcaacgc cgacggcagc acccgccagg cctacgacac ggccggaacc
     7981 ggccgcgtgc agtaccagta cgacgcggtg gtcgccgtct cggaccgctt cgagggcccg
     8041 tactcacggc gatggaccgc cggggtcggc gccggccaca acaacttctt caccgactcc
     8101 gacggcaccg tgtgggccac cttcttccgc aacccggcgt tcggccactg gtccgacccg
     8161 tcgcgcgtcg ccgacgccgc cgtgcccggt gtcgtacggg tggagtggac cggcccgcag
     8221 ggcaaccgcc tgtacgtccg gcggcgggac ggcgggcgcg gctgacgggt caggcggagg
     8281 cgccccgggg ccgccgtccg cgcgggagcg ggcagaagcg actgcccccc tcggtcacga
     8341 cggtcgcccc gggggcgatc tcggtgaaac cggcgtcgac caccaccggc aacccgctcg
     8401 cggtgagttc ccgccagctc tcgggcgtcg cgacggcgac cgagagcggg aagccggcct
     8461 cgcgccactc ctcgcgctcc gcgtcggaca gctcccacca ggcgagctgc gcgccgtgtc
     8521 cggcctgcgc catcgtcttg cccgccgaca tgtccggccc ggggttgagc cagagcaccg
     8581 ggccggtccc gtcgggcgcg ggcggcggga cggggtcgtc cagctccgta ccggacacct
     8641 ggagcttggc cagctccttg ggccagccgt ccagagggat cggcgggaag acccgtacct
     8701 cggcgctctc gcccgtgacc gtgatcccgg gcagtgccga ggccttgcgc cactccgcgc
     8761 cgcgcgcccg gcgcaccacc ttgcggatcc gggcgtcctc ccagtcccgt acggcctggg
     8821 cccactcccc gtcgcccagc gaacgctcgt ccgagagcat cgtgagcacc gcgcgggcgg
     8881 cggtccgcac cgcgtcggtg cgccccgggg gatcggtctt ctccaggtgc accaccagcg
     8941 gaagcacgta ctgcggtgcc tcgtcgcggg cggtcggctc accgcggaac gggctgtccg
     9001 cgggcggggc ggaaaggggc cgggaaacgt cgtcactgct cacggaccca gtctgccagg
     9061 cctccggaca gcacttcttg gcggaacaca cggctccggg tgaggatgca cgccatgaag
     9121 agcgacctct tttccagcga gcacatggcc cagcaggcca ccgcccccgg tatgaccctg
     9181 cagaacgcca aatccatcaa gtacgccgtc gacggcgaga tgcacgcgcg ccagggatcg
     9241 atgatcgcct tccgcgggaa cctccagttc gagcgcaagg gccagggcat cggcggcatg
     9301 ctcaagcgcg cggtcaccgg cgagggcctg ccgctcatgg cggtgcgcgg ccagggcgag
     9361 gcctggttcg cccacgaggc ggccaactgc ttcatcgtgg acatggagca gggtgacgtc
     9421 ctgaccatca acggccgcaa cgtgctgtgc ttcgacgcca ccctcgccta cgagatcaag
     9481 accgtgaagg gcgcagggat gaccggcggc ggcctcttca acagcgtctt caccggccag
     9541 ggcaagctcg gcctcatgtg cgacggccac cccatcgtga tccccgtcag cgcccggcag
     9601 ccggtctacg tcgacacgga cgcggtcgtc ggctggagcg cccagctctc cacctcgctg
     9661 caccggtccc agagcttcgg ctcgatggtg cgaggcgggt ccggcgaggc ggtccagctg
     9721 atgctccagg gcgaggggtt cgtgatcgta cggcccagtg aggtcaagca ggagaaggcg
     9781 tcggcgaact gaacccgcac ggggccggcc gccgtcacgg ccttcgcggg gagcgggtgc
     9841 tgcgcggggt cggcctcgtg ctgcccccgg cacgccggtg cgcgtcgagg gcgccaacgg
     9901 cacgggcagg tcggccgctc ccggcgtccg ccagggccgt gctcgccgcc gccgcggtgg
     9961 ccgccggcac cgcccggctc gcctcgcggc gggagtgagt acgctcgacg gcatggacga
    10021 cgcgtactgc gagacgcccg ctccggcgcc cgtacccgag gacacgggcg ggccgtatgc
    10081 cgagtgcgtg ctgtgccggg agcccaccga gtatccggag tcgacgaagg gcgccaccct
    10141 ctgcccggtc tgcgcctggc aggaggcggg ccgcacggcc tgctccggct gacggaggcc
    10201 cacttcctca gccgcgcatc agctcggaga ccttcacgaa gcggtacccc cgctcgcgca
    10261 gcgccgggac cacctggcgt acggcctcgt cggtgacggg cgccgcgctg cgcgtgcagt
    10321 gcatgaccac cagcgaaccc ggcccgacgc cgtccagcac ctgctgggcc accgcgtccg
    10381 cgtccgtggc gaaggcgtcg ccgctgacga cgtcccactg caccgccgtc acacccgtcg
    10441 gcgccagggc cttgcgcgag gcgtcgtcgt agcagccgcc cgggaagcgg aagtacggca
    10501 ccacctggcg tgcccccgtc ttccggatcg cgtcgaacgc ctgctccacc tcgccccgca
    10561 tggcgccctc ctcgacggtc ggcagcccgt agcaggggga ggagaaggcg tagtggctgt
    10621 aggagtggtt ggcgatctcg aacagcggat cggtgccgat cgagcgggcc tgggacgggt
    10681 actcctcggc ccaccggccg gtcatgaaga ccgtcgccgg caccttcagc cggcgcagca
    10741 gcgcgatcag ctccgggttg tcgaagtgct cgcccgcctc ggcgcgcggc ccctgatccg
    10801 ccgtcatgtc ggcgtcgaag gtgagcgcca cgaccttctc gtcccgcccc ggggaaccgg
    10861 tggccgagcc ggagccgccc ggcgcccggc gttcgaacac cggggtgaga ccggccggcc
    10921 cgggggccat cgtgggcggc ttctccaccc gctccggggc cgccttcgcc accggactcg
    10981 gactcgccgg gggcaccagg gtctcggtac gggcggcccc tgggccgccg cagcccgtga
    11041 gtgccatccc gcccagaatt accagtgctg tcatcctact aaccgatttg atcactggag
    11101 gacgttagct gacggcccgt cggatctggt cgcggcgctc cgggggagcg gtgctccggg
    11161 ggagcggtga acgccgctca gtgccagggc cccgtcaccg cgaacgtggt ccccggggtg
    11221 tagcagttca cgaacatcgt ggcgccgtcc ggcgcgaacg tgaccccggc gaactcgccc
    11281 cactcgggct catcggcggt cccgatgtcc t
//
