LOCUS       NC_015953              10399 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. SirexAA-E, complete sequence.
ACCESSION   NC_015953
VERSION     NC_015953.1
KEYWORDS    GSC:MIGS:2.1; RefSeq.
SOURCE      Streptomyces sp. SirexAA-E
  ORGANISM  Streptomyces sp. SirexAA-E
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7414440)
  AUTHORS   Takasuka,T.E., Acheson,J.F., Bianchetti,C.M., Prom,B.M.,
            Bergeman,L.F., Book,A.J., Currie,C.R. and Fox,B.G.
  TITLE     Biochemical properties and atomic resolution structure of a
            proteolytically processed beta-mannanase from cellulolytic
            Streptomyces sp. SirexAA-E
  JOURNAL   PLoS One 9 (4), e94166 (2014)
   PUBMED   24710170
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414440)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Ovchinnikova,G., Davenport,K., Detter,J.C., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Adams,A., Raffa,K., Adams,S., Book,A., Currie,C. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Streptomyces sp. SirexAA-E
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7414440)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Ovchinnikova,G., Davenport,K., Detter,J.C., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Adams,A., Raffa,K., Adams,S., Book,A., Currie,C. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2011) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. SirexAA-E
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            environment                       :: Soil, Terrestrial
            num_replicons                     :: 1
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Free living
            trophic_level                     :: Missing
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: Missing
            sequencing_meth                   :: WGS
            assembly                          :: Newbler v. 2.3 (pre-release)
            finishing_strategy                :: Finished
            GOLD Stamp ID                     :: Gi05895
            Funding Program                   :: DOE-GLBRC 2008
            Source of Isolate :: Cameron Currie (currie@bact.wisc.edu)
            Cell Shape                        :: Filament-shaped
            Motility                          :: Nonmotile
            Sporulation                       :: Sporulating
            Temperature Range                 :: Mesophile
            Gram Staining                     :: Gram+
            Diseases                          :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Finished
            Current Finishing Status          :: Finished
            Assembly Method                   :: Newbler v. 2.3
            Genome Coverage                   :: 30x
            Sequencing Technology             :: 454/Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000177195.2-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 02:12:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,619
            CDSs (total)                      :: 6,532
            Genes (coding)                    :: 6,385
            CDSs (with protein)               :: 6,385
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 61 of 147
            Pseudo Genes (incomplete)         :: 120 of 147
            Pseudo Genes (internal stop)      :: 8 of 147
            Pseudo Genes (multiple problems)  :: 39 of 147
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:50:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1441157
            Orig. end                         :: 1451556
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP002993.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4086644
            Source DNA and organism available from Cameron Currie
            (currie@bact.wisc.edu)
            Contacts: Cameron Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="7"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(340..1440)
                     /locus_tag="SACTE_RS06305"
                     /old_locus_tag="SACTE_1282"
     CDS             complement(340..1440)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="SACTE_RS06305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1282"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_014045191.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 9.3e-19, bitscore:
                     66.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHEAARILRIKGRVLLGPDDVRDEIWAVDGRITHERPPGADGA
                     AALSGWALPGLVDAHCHVGLDGHGPVDAATSEKQALTDREAGTLLIRDAGSPSDTRWID
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEDALRDLVEAGIDCVEHATGLTEDTI
                     PLFAERGVAIVPTLVNIATFPDLAAGGEARFPRWSAHMRQLYERRYDTVRAAYDAGIPV
                     FVGTDAGGSLAHGLVAAEVAELVKAGIPPLDALSATAWGARRWLGRPVLEEGAPADLVV
                     YDEDPRADVRVLAGPRQVVLNGRVIG"
     gene            1553..2659
                     /locus_tag="SACTE_RS06310"
                     /old_locus_tag="SACTE_1283"
     CDS             1553..2659
                     /NRPS_PKS="Domain: Aminotran_5 (18-291). E-value: 1.1e-26.
                     Score: 85.5. Matches aSDomain:
                     nrpspksdomains_SACTE_RS06310_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045192.1"
                     /locus_tag="SACTE_RS06310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1283"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_014045192.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7e-20, bitscore:
                     69.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADHFAAIERRVAGLLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLVVPFHAAVTAEQVGQALAAH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERLADNPRAPRRSYLSLLDWKDRWIDAGRTALPHAP
                     AQLEMLALDACVERIEAEGLDTLTARHASAAAATRAGASALGGGLEPYVHEARDAAPVA
                     TTLRVPAGTDATALVRSALAADPSLPLVAGGGALAREMIRVNHYGVDATRGAVLSSLAA
                     LGSALTEAGRTTDLDAAREAVSETWAND"
     aSDomain        1607..2425
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SACTE_RS06310_Aminotran_5.1"
                     /evalue="1.10E-26"
                     /label="SACTE_RS06310_Aminotran_5.1"
                     /locus_tag="SACTE_RS06310"
                     /protein_end="291"
                     /protein_start="18"
                     /score="85.5"
                     /tool="antismash"
                     /translation="IERRVAGLLATEQDVVITQGEALLPLEGCIRGGARPGSTALNVVT
                     GPYGQTFGNWLRDCGAEVVDLVVPFHAAVTAEQVGQALAAHPEIDFVSLVHAEAATGNT
                     NPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVSV
                     SARAWERLADNPRAPRRSYLSLLDWKDRWIDAGRTALPHAPAQLEMLALDACVERIEAE
                     GLDTLTARHASAAAATRAGASALGGGLEPYVHEARDAAPVATTLRVPAGTD"
     misc_feature    2689..2702
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.39"
                     /tool="antismash"
     misc_feature    2819..2836
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    complement(2821..2836)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2831..2846
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2973..2982
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.08"
                     /tool="antismash"
     gene            3001..3531
                     /gene="ectA"
                     /locus_tag="SACTE_RS06315"
                     /old_locus_tag="SACTE_1284"
     CDS             3001..3531
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607778.1"
                     /locus_tag="SACTE_RS06315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1284"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_014045193.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLTIDTPRVEDGAALWRIARDSEVLDLNSSY
                     SYLLWCRDFAATSVVARDEKGEAVAFVTGYIRPDRPETLVVWQVAVDDAHRGKGLAGEL
                     LDALTARVSAEQGLASLETTITPDNTASDRLFTSYAQRHDVALEREVLFDGELFPEGTH
                     LPEVLYRIGPFHA"
     gene            3674..4936
                     /gene="ectB"
                     /locus_tag="SACTE_RS06320"
                     /old_locus_tag="SACTE_1285"
     CDS             3674..4936
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 1.1e-71.
                     Score: 233.5. Matches aSDomain:
                     nrpspksdomains_SACTE_RS06320_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="SACTE_RS06320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1285"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_014045194.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.2e-77, bitscore:
                     257.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFESLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFENVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQDLCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKGELDIWEPGEHNGTFRGNNPAFVTAAATLDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEATAQFRGRGLVWGLEFTDPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARCVRETA"
     aSDomain        3758..4762
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SACTE_RS06320_Aminotran_3.1"
                     /evalue="1.10E-71"
                     /label="SACTE_RS06320_Aminotran_3.1"
                     /locus_tag="SACTE_RS06320"
                     /protein_end="363"
                     /protein_start="28"
                     /score="233.5"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFENVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQDLCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKGELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDAYWADGQMEKQTLARGEQVEQTLLAICAEEATAQFRGRGLVWGLEFT"
     gene            5001..5399
                     /locus_tag="SACTE_RS06325"
                     /old_locus_tag="SACTE_1286"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="SACTE_RS06325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1286"
                     /product="ectoine synthase"
                     /protein_id="WP_014045195.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.5e-53, bitscore:
                     177.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDQHIKAASGTWESKRIVLAKEKVGFSLHETVMY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWITPGTMYLLDGHEHHTMRPKTDFR
                     CVCVFNPPVTGREEHDENGVYPLLTEEA"
     gene            5403..6296
                     /gene="thpD"
                     /locus_tag="SACTE_RS06330"
                     /old_locus_tag="SACTE_1287"
     CDS             5403..6296
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="SACTE_RS06330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1287"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_014045196.1"
                     /sec_met_domain="PhyH (E-value: 9.3e-38, bitscore: 129.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQVRTDLYPTRGAAEMTTPRQDPVIWSAPGAPGPIAAKDLQSF
                     EHDGFLAIDQLITPDEVAGYHDELDRLIADPAVRADERSIIEPKSQSVRSVFEVHKISE
                     VFARLVGDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKSFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENTAEEPFSAP
                     IRRPEFIGARDFTPVK"
     gene            6454..7467
                     /locus_tag="SACTE_RS06335"
                     /old_locus_tag="SACTE_1288"
     CDS             6454..7467
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104671.1"
                     /locus_tag="SACTE_RS06335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1288"
                     /product="VOC family protein"
                     /protein_id="WP_019992816.1"
                     /transl_table=11
                     /translation="MTSLVQHITVDCADAYELAVFWAKVLDSSVSDDDAPGDPEALVEA
                     PGVALLFVTVPEPKRAKNRVHLDVRPTDRTRDEEVERLLALGATLAADHRKPDGRGWAT
                     LADPEGNEFCVECGAAERAASAGVRLPVTADDVTLAVRLAADTLAKSPVDGWRAPAGTL
                     TWDCWETVEHLSDDLFAYAVQLGPRKPPLKHEVPYRWAPGREGGPWNAVFADPEAGTVG
                     LLETLEASGALLTAMVRTASPDLRSYHSHGVSDPEGFAAMGVVETLVHTHDVAAGLSLA
                     WTPPRDLCDRVLARLFPEAPDDADRWTVLLWCTGRADLPGRERVTSWKWHGAPLDR"
     gene            7580..8089
                     /locus_tag="SACTE_RS06340"
                     /old_locus_tag="SACTE_1289"
     CDS             7580..8089
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045198.1"
                     /locus_tag="SACTE_RS06340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1289"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_014045198.1"
                     /transl_table=11
                     /translation="MEQPPPPPLTDQATDQAPADPAATIDGMSATQPIGYWSGLVHAAV
                     TRRLRDAMAGIDVTQPQYWVLNRVRDGPAAPTREEVAAQLTPLADGPHDIARVVDQLLY
                     RGWLAAGAEGRLRLTDDGEAARVRLRELVTGVRAEVHRGIGDEEYVAALKVLRRMAANI
                     EGSGAL"
     gene            complement(8118..9680)
                     /locus_tag="SACTE_RS06345"
                     /old_locus_tag="SACTE_1290"
     CDS             complement(8118..9680)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576697.1"
                     /locus_tag="SACTE_RS06345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_1290"
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="WP_014045199.1"
                     /transl_table=11
                     /translation="MYGTTHHTRRRAAGAVAALTGLLLAALPAQAAHAATTPANPGFES
                     GATGWSTYSATGQDAASFTEAGGHGGSTRLSHWSASAYKVETYQHLTGLADGTYTLSAW
                     VRSGGGQNAAYIALRDCGSAEQRTDLPPTAGSDWVRIVTSVKVTGGACTISLDSDARAG
                     QWANFDDITFTPGSTGLSVRGGDLSTLPKNEAHGAVYRDAGGRAGDAMSILENSGMNYV
                     RLKVWVDPADGYNDKAHVLAMAKRAKALGMKTLVDFHYSDAWADPGKQNKPAAWAGHDY
                     GRLRTDVHDHTYDVLNALKAQGTTADMVQIGNETNAGMLWPEGSTSNWSQLAGLLTSGA
                     NAAKAVSSTTKVVLHLAEGGDNAGTRWWFDNAVAQKVPFDVIALSYYGYWHGSLTELQT
                     NLDDAASRYGKPVMVAETAYAHTLADADGLENNVATSDQLVAGYPATPAGQAASLRDVM
                     SVVEAVPNGRGLGAVYWEPAWTAVTGSGWDPADPSSGNAWENQAVFGYDGTLLPAAGWF
                     SHR"
ORIGIN
        1 gctgttcaag atggtggaac ccccacaaga gacatggagc caccggcacc gcccaatggg
       61 ggacatcgcg ggcgccggtg gcctcgactc cgtgaatctt tacgcacgga gggtgaactg
      121 acagacacct ggcgtcagtt cactccaaag gggggtttcc gcgtacgtat tcgaatcctt
      181 cggcacagga gttccccgga gtcacccgtt tcaccgagcg cgggcggcgc accggccgcc
      241 tcggcccgac gaggcacgaa agggtccaac gagccagggc ggcaccaggt caccgccggt
      301 caacatcgca cccacacccg cgtcggccgc cgcggcccgt cagccgatga cgcggccgtt
      361 cagcaccacc tgccggggac ccgccagcac gcgtacgtcg gcgcgcgggt cctcgtcgta
      421 caccaccagg tcggccgggg ccccttcctc cagcaccgga cggcccagcc agcgtcgcgc
      481 gccccaggcc gtcgcggaga gcgcgtccag gggcgggatg cccgccttga ccagctcggc
      541 gacctcggcg gccaccagac cgtgggccag cgagccgccc gcgtcggtcc cgacgaagac
      601 ggggatgccc gcgtcgtagg ccgcccgcac ggtgtcgtaa cggcgctcgt acagctgccg
      661 catatgggcc gaccagcggg ggaacctggc ctcgccgccc gccgcgaggt ccgggaaggt
      721 ggcgatgttg accagggtgg ggacgatcgc cacgccgcgc tcggcgaaga gcgggatggt
      781 gtcctcggtg agtcccgtcg cgtgctcgac gcagtcgatc ccggcctcca cgagatcgcg
      841 cagggcgtcc tcggcgaagc agtgcgcggt gacccgggcg ccgaggcggt gcgcctcggc
      901 gatggcggcc tcgacctcgc cgcgcggcca gcaggcggtc aggtccccgg cgtcccggtc
      961 gatccagtcg ccgaccagct tcacccagcc gtcgccgcgc cgcgcctccc gggcgacgta
     1021 ggcgaccagg tcaccgggtt cgatctcgtg cgcgtagttg cggatgtacc tgcgggtccg
     1081 ggcgatgtgc cggcccgccc ggatgatctt cggcaggtcc tcccggtcgt cgatccaccg
     1141 ggtgtcggag ggcgagcccg cgtcccggat gagcagggtg cccgcctccc ggtcggtgag
     1201 cgcctgcttc tcgctcgtcg ccgcgtcgac cggtccgtgc ccgtcgaggc cgacgtggca
     1261 gtgggcgtcg acgaggccgg gcagcgccca gccggagagg gccgccgcac cgtcggcgcc
     1321 cggcggccgc tcgtgggtga tccgcccgtc cacggcccag atctcgtccc tgacgtcgtc
     1381 ggggccgagc agcacccgcc ccttgatccg cagtatccgc gccgcctcgt gatcgctcat
     1441 gcacagcact gtacgaggca cggcaccggg ggccgccggg tgtgctgggt actctcggac
     1501 gcacgcgccc ccgagcgccg accgcgaacg acacacctga agagagcacc ccgtgacgca
     1561 ccccttcctc gacctcccgc cgctgaccgc cgaccacttc gcggcgatcg agcggcgggt
     1621 ggccggcctc ctcgccaccg agcaggacgt cgtcatcacc cagggcgagg cgctgctgcc
     1681 cctcgaaggc tgcatccggg gcggcgcccg gcccggctcg accgcgctga acgtcgtgac
     1741 gggtccgtac ggccagacct tcggcaactg gctgcgggac tgcggcgcgg aggtcgtcga
     1801 cctggtggtg cccttccacg cggcggtcac cgcggagcag gtcggacagg cgctggccgc
     1861 gcacccggag atcgacttcg tgtcgctggt gcacgcggag gcggcgaccg gcaacaccaa
     1921 cccggtcgcg gagatcggtg aggtggtccg ggcgcacggg gccctcttca tgctggacgc
     1981 cgtggcgtcg gtgggcgccg agcccctgct gccggacgcc tggggcgtgg acctgtgcgt
     2041 gatcggggcg cagaaggcga tgggcggccc ggccggtgtc tcggcggtgt cggtgagcgc
     2101 gcgggcgtgg gagcggctgg cggacaaccc gcgggcgccc cgccggtcct acctctcgct
     2161 gctggactgg aaggaccggt ggatcgacgc gggccgcacg gctcttccgc acgccccggc
     2221 ccagctggag atgctggcgc tggatgcctg tgtggagcgg atcgaggcgg agggcctgga
     2281 cacgctgacg gcccggcacg cctcggcggc ggccgccacc cgggcgggcg cgtccgccct
     2341 cggcggcggg ctggagccgt acgtccacga ggcccgggac gccgcgccgg tcgccacgac
     2401 gctgcgcgtg ccggccggta ccgacgccac cgcgctcgtc cggagcgcac tcgcggcaga
     2461 cccgtcgctg cccttggtgg cgggcggcgg ggcgctcgcc cgggagatga tccgggtcaa
     2521 ccactacggc gtggacgcga cacggggcgc cgtgctctcg tcgctggccg ccctgggctc
     2581 cgcgctcacc gaggcgggcc ggacgaccga cctggatgct gcccgcgaag ccgtatcgga
     2641 aacatgggcg aacgactgag gcgaatatgc cggacggaac cggcacacat atgaaaagaa
     2701 tagcggggag ctgctttatc gagagcagct ccccgcattc ttttgcccgc aatcaccgga
     2761 cctaaacgcc ccactttcga gggacggccg atctacgtaa tccggacacg tgtcaggaga
     2821 gttacaaccc tgtgaccgac tccacagaga cggcgttatg ccccttaaat tccggtcaaa
     2881 tcacgagaaa tcgccgcccg cttcggctcg actcgcgccc gcgtgatagc acagacacgt
     2941 gcgccaccca acccttcccg gcgatgcaat ttcgattttt gctgggtaaa ttcaattcgc
     3001 atgaccgccg caccagcaga ttttgcccgt gcccgaagcg aattccttac aatcgacacc
     3061 ccgcgagtgg aggacggagc cgcgctctgg cgtatcgccc gcgactccga ggtcctggac
     3121 ctcaactcct cgtacagcta cctgctgtgg tgccgtgact tcgcggcgac ctccgtggtc
     3181 gcccgcgacg agaagggtga agccgtcgcc ttcgtgacgg ggtacatccg gcccgaccgc
     3241 cccgagacgc tcgtcgtctg gcaggtggcc gtggacgacg cccaccgcgg gaaggggctg
     3301 gccggtgagc tgctggacgc cctgaccgcc cgtgtctcgg ccgagcaggg cctggcgtcc
     3361 ctggagacga ccatcacccc ggacaacacc gcgtccgacc ggctgttcac ctcctacgcg
     3421 cagcgccacg acgtggccct ggagcgcgag gtgctcttcg acggcgagct gttccccgag
     3481 ggaacgcacc tgccggaggt gctgtaccgc atcggcccgt tccacgcgta ggaccgtcgc
     3541 ccgccccgcc ggggcggcac gtactaccgg catcgggtcc cgcccccgcc cggggcgcgt
     3601 acccggcccg gccccgcccg gggcccgcac gcctcacccg ccttccgtca caccctcgat
     3661 caggagatcc gctgtgacca tcaccccgcc cgccctgagt gtcttcgaga gtctcgaatc
     3721 ggaagtacgc agctactgcc gcggctggcc cgccgtgttc gaccgcgcgc agggcgcccg
     3781 cctgaccgac gaggacggcc actcctacct cgacttcttc gccggggccg gatcgctcaa
     3841 ctacggccac aacaacccgg tgctgaaacg cgcgctgatc gactacatcg aacgcgacgg
     3901 catcacccac ggcctcgaca tggccacgac ggccaaacgc gcgttcctgg agacgttcga
     3961 gaacgtgatc ctgcgcccgc gcgacctgcc gtacaaggtg atgttccccg gcccgaccgg
     4021 caccaacgcg gtcgaggcgg cgctgaagct cgcccgcaag gtcaagggcc gcgagtccgt
     4081 cgtctccttc accaacgcct tccacggcat gtcgctgggg tcgctcgcgg tgacgggcaa
     4141 cgccttcaag cgcgccggtg cgggcatccc gctggtgcac ggcaccccga tgccgttcga
     4201 caactacttc gacggcacgg tccccgactt cctctggttc gagcggctcc tggaggacca
     4261 gggctccggc ctcaacaagc ccgccgcggt gatcgtggag acggtgcagg gcgagggcgg
     4321 catcaacgtg gcccgcgccg agtggctgcg tgccctccag gacctgtgcc accgccagga
     4381 catgctgctg atcgtcgacg acatccagat gggctgcggc cgtaccggcg gcttcttctc
     4441 cttcgaggag gccggcatcg tcccggacat cgtcacgctg tcgaagtcca tcagcgggta
     4501 cggtctgccg atgtcgctct gcctcttcaa gggcgagctg gacatctggg agccgggcga
     4561 gcacaacggc accttccgcg gcaacaaccc ggcgttcgtc accgccgccg ccacgctcga
     4621 cgcctactgg gcggacggcc agatggagaa gcagaccctg gcccgcggcg agcaggtgga
     4681 acagacgctg ctggcgatct gcgccgagga ggccaccgcg cagttccgtg gccgcggtct
     4741 cgtctggggc ctggagttca ccgacccggc acgtgcctcc gccgtctgcg cgcgcgcctt
     4801 cgagctgggc ctgctgctcg agacgtcggg cccgcagagc gaggtcgtga agctgctgcc
     4861 gccgctgacc atcacccccg aggagctgga cgagggcctg cgcacgctgg cccgctgcgt
     4921 ccgcgagacc gcctgacccg ggcagcgccc gggacaggca ccgattgagc aggacaagaa
     4981 gagaaaggca ccatcccacc gtgatcgtcc gatcgttcag tgacatcgag aacaccgacc
     5041 agcacatcaa ggcggcctca ggcacctggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtgggctt ctcgctccac gagaccgtga tgtacgcggg cacggagacc tccatgtggt
     5161 acgcgaacca catcgaggcg gtgctctgca ccgagggtga ggccgaactc accaacgacg
     5221 agaccggcga gaagcactgg atcacgccgg gaacgatgta cctgctggac gggcacgagc
     5281 accacaccat gcggccgaag accgacttcc gctgcgtctg cgtcttcaat ccgcccgtca
     5341 ccgggcggga ggagcacgac gagaacggtg tctacccgct gctgacagag gaggcctgaa
     5401 ccatgaccac ccaggtacga accgacctct accccacgcg cggcgccgcc gagatgacca
     5461 ctccccgcca ggacccggtc atctggtccg cgccgggcgc gcccggaccg atcgccgcga
     5521 aggacctcca gtccttcgag cacgacggct tcctcgccat cgaccagctc atcacaccgg
     5581 acgaggtggc cggctaccac gacgagctgg accggctgat cgccgatccg gcggtccgcg
     5641 ccgacgagcg ttcgatcatc gagccgaagt cgcagagcgt gcggtccgtc ttcgaggtcc
     5701 acaagatcag cgaggtcttc gcccgtctgg tcggcgacga gcgtgtggtg ggccgggccc
     5761 gccagatcct cggctccgac gtgtacgtcc accagtcccg gatcaacgtg aagccgggct
     5821 tcggcgcctc cggcttctac tggcactcgg acttcgagac ctggcacgcc gaggacggtc
     5881 tgccgaacat gcggaccgtg tcggtctcga tcgcgctgac ggagaacttc gacaccaacg
     5941 gcgggctgat gatcatgccc ggttcgcaca agtcgttcct cggctgcgcg ggcgagacgc
     6001 cgaaggacaa ctacaagaag tccctgcaga tgcaggacgc cggcaccccg tccgacgagg
     6061 cgctgacgaa gatggccgac cggcacggca tcaagctctt cacgggcaag gccggttcgg
     6121 cgacctggtt cgactgcaac gcgatgcacg gctcggggga caacatcacc ccgtacgccc
     6181 gcagcaacgt gttcatcgtg ttcaacagtg tggagaacac ggccgaggag cccttctcgg
     6241 ctccgatccg ccgccctgag ttcatcgggg cgcgggactt cacgccggtg aagtagccgt
     6301 agggcggttc gaaggggcgg gacgcggtag gcgtcccgcc ccttccgcgt gccgtcggcc
     6361 cggcgcgggc gcgcggtctt ccccggccct ggctcgcgga cgcgtccgta actcgctgga
     6421 ccccggcccg tgccggggga gacagtggcg tccatgactt cgctcgtaca gcacatcacc
     6481 gtcgactgcg ccgacgccta cgaactggcc gtcttctggg ccaaggtgct cgactcgtcc
     6541 gtgtccgacg acgacgcgcc cggtgatccg gaggcgctgg tcgaggcgcc gggggtggca
     6601 ctgctcttcg tcacggtgcc cgaacccaag cgcgccaaga accgcgtcca cctggacgtg
     6661 cggcccacgg accgcacccg cgacgaggag gtcgagcggc tgctcgccct cggagccacc
     6721 ctggccgccg accaccgcaa gccggacggc cggggctggg cgaccctggc cgaccccgag
     6781 ggcaacgagt tctgtgtgga gtgcggcgcc gcggagcggg ccgcgtcggc cggggtccgg
     6841 ctgcccgtca ccgcggacga cgtgacgctg gcggtgcggc tcgcggccga caccctcgcg
     6901 aagtccccgg tggacggctg gcgtgcccct gcggggacgt tgacgtggga ctgctgggag
     6961 acggtggagc acctgagcga cgacctcttc gcgtacgccg tccagctcgg tccgcgcaaa
     7021 cccccgttga agcacgaggt cccctaccgc tgggcccccg ggcgcgaggg cggcccctgg
     7081 aacgcggtct tcgcggaccc ggaggcgggg accgtcgggc tgctcgagac gctggaggcg
     7141 agtggcgccc tcctgaccgc gatggtgcgg acggcctctc ccgatctgcg ttcctaccac
     7201 tcgcacgggg tctccgaccc ggagggcttc gccgcgatgg gtgtggtgga gaccctcgtg
     7261 cacacccatg acgtcgccgc ggggctctcg ctcgcctgga caccgccccg ggacctctgc
     7321 gaccgggtcc tggcccggct cttccccgag gcgccggacg acgccgaccg gtggaccgta
     7381 ctgctgtggt gcaccggccg cgcggacctg cccggccgtg agcgggtcac ctcgtggaag
     7441 tggcacggag cgcccctgga ccgctgaggg cgcccgtatc cggccgtctt ccggtcgtct
     7501 tccggtcgtc tccggccgga agaggccggt cggggcggca cgcggccgtc ccgaccgggg
     7561 cggctgtacc gtcgcggcca tggaacagcc gccccctccc ccgctcacgg accaggcgac
     7621 ggaccaggcg cccgccgacc ccgccgccac catcgacggc atgtcggcca cccagcccat
     7681 cgggtactgg agcggtctgg tccacgccgc cgtcacccgg cgcctgcggg acgccatggc
     7741 cgggatcgat gtcacccagc cccagtactg ggtgctgaac cgggtgcgcg acgggcccgc
     7801 ggcgcccacc cgcgaggagg tcgcggccca gctcacgccc ctggccgacg gaccgcacga
     7861 catcgcccgg gtcgtcgacc aactgctcta ccgcggctgg ctcgccgccg gtgccgaggg
     7921 gcggctgcgc ctcaccgacg acggggaggc cgcccgggtg cggctgcgtg agctggtgac
     7981 cggtgtccgc gccgaggtgc accggggcat cggcgacgag gagtacgtgg ccgcgctgaa
     8041 ggtgctccgc aggatggcgg ccaacatcga gggttcgggc gccctctgac agcggctgcc
     8101 ggacggcgcc cgtcggtcta ccggtgcgag aaccagcccg ccgccggcag cagcgtcccg
     8161 tcgtagccga acaccgcctg gttctcccag gcgttgcccg acgacgggtc ggccgggtcc
     8221 cagccgctgc cggtcacggc ggtccaggcc ggttcccagt agacggcgcc gagtccacgg
     8281 ccgttcggaa cggcctcgac gacgctcatg acgtcgcgga ggctggccgc ctggccggcc
     8341 ggggtcgccg ggtagccggc gacgagctgg tcggaggtgg cgacgttgtt ctccaggccg
     8401 tcggcgtcgg ccagcgtgtg ggcgtacgcc gtctcggcga ccatcaccgg cttgccgtag
     8461 cgggacgcgg cgtcgtccag gttggtctgg agctcggtca gtgagccgtg ccagtagccg
     8521 tagtaggaga gggcgatgac gtcgaagggc accttctgcg ccaccgcgtt gtcgaaccac
     8581 cagcgggtgc ccgcgttgtc gccgccctcc gcgaggtgca ggacgacctt cgtggtggag
     8641 gagaccgcct tggcggcgtt cgcgcccgag gtgaggagtc cggccagctg ggaccagttc
     8701 gaggtggagc cttcgggcca gagcattccg gcgttcgtct cgttgccgat ctgcaccatg
     8761 tcggcggtgg tgccctgggc cttcagcgcg ttcagcacgt cgtaggtgtg gtcgtggacg
     8821 tcggtgcgca gccggccgta gtcgtgcccg gcccaggcgg cgggcttgtt ctgcttgccg
     8881 gggtcggccc aggcgtcgga gtagtggaag tcgacgagcg tcttcatgcc gagcgccttg
     8941 gcgcgcttgg ccatggccag gacgtgggcc ttgtcgttgt agccgtcggc cgggtccacc
     9001 cagaccttga ggcggacgta gttcatcccg gagttctcga ggatgctcat ggcgtccccg
     9061 gcccggccgc ccgcgtcgcg gtagaccgcg ccgtgcgcct cgttcttcgg gagggtggag
     9121 aggtcgccgc cgcgcacgct cagcccggtg gagcccgggg tgaacgtgat gtcgtcgaag
     9181 ttggcccact gcccggcccg cgcgtcggag tcgaggctga tcgtgcaggc cccgccggtc
     9241 accttcacgg aggtgacgat ccgcacccag tcgctcccgg cggtcggcgg caggtcggtg
     9301 cgctgctcgg cggaaccgca gtcgcgcagg gcgatgtagg ccgcgttctg gccgccgccg
     9361 gagcggaccc aggcgctgag ggtgtacgtg ccgtcggcga gcccggtcag gtgctggtag
     9421 gtctcgacct tgtaggccga ggccgaccag tggctgagcc gggtggagcc gccgtggccg
     9481 ccggcctcgg tgaaggaggc ggcgtcctgg ccggtggccg agtacgtcga ccagccggtg
     9541 gcccccgact cgaaaccggg gttcgcgggg gtggtggccg cgtgggcggc ctgggcgggc
     9601 agggcggcca gcagcaggcc ggtcagtgcg gccaccgcgc ctgcggctct cctgcgggtg
     9661 tggtgcgtcg ttccgtacat cgtcgatgtc ccttcgagcg ttgtgcgggg taacggactt
     9721 gggtgcgcct cagccgtcga gccgtacgac gcggaccgcg cccgccggca cggcgagcgt
     9781 ctccaccacg ccctcgccgg tgaggagttc ggtgccgggg ccgtcgaggt gcacgtcggc
     9841 cccgcggtcg gagtggttga ccgcgaagag gtaggtgccg ctctcccccg tgcggcgcac
     9901 gacctcgacg tcccgggcga gaccggtgcg cggcgcgaga cccgcgtcgg cgagcgcccg
     9961 gcccagcacg gtgtccaggt ccgggccgct cagccgggtg gagacgtacc aggcgctgcc
    10021 ctcgccgagc cggttgcggg tgatcgcggg acggccggcc gggacgccgt cggcgtacga
    10081 ccagaggggc tcggcgccgc gcgggatcac cacgtccgac cagacgtcac cggtcagctc
    10141 cgggccctcg ggcgcgccct cgggggtggt caggcggacc gtgccgccgt cgaggagggg
    10201 ggagaactcc tcgacggtca ggcccaggac gtcccgtagg gcgccggggt agggaccggg
    10261 gtgcacggcg tcgtcggcgt cgacgatccc ggagaagtac gagacgacga gggtgccgcc
    10321 gccctcgacg taacggcgca ggttgcggcc ggtctcctcg gtggcgaggt agagggcggg
    10381 gacgacgacc agggggtag
//
