LOCUS       NC_015953              10531 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. SirexAA-E, complete sequence.
ACCESSION   NC_015953
VERSION     NC_015953.1
KEYWORDS    GSC:MIGS:2.1; RefSeq.
SOURCE      Streptomyces sp. SirexAA-E
  ORGANISM  Streptomyces sp. SirexAA-E
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7414440)
  AUTHORS   Takasuka,T.E., Acheson,J.F., Bianchetti,C.M., Prom,B.M.,
            Bergeman,L.F., Book,A.J., Currie,C.R. and Fox,B.G.
  TITLE     Biochemical properties and atomic resolution structure of a
            proteolytically processed beta-mannanase from cellulolytic
            Streptomyces sp. SirexAA-E
  JOURNAL   PLoS One 9 (4), e94166 (2014)
   PUBMED   24710170
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414440)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Ovchinnikova,G., Davenport,K., Detter,J.C., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Adams,A., Raffa,K., Adams,S., Book,A., Currie,C. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Streptomyces sp. SirexAA-E
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7414440)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Ovchinnikova,G., Davenport,K., Detter,J.C., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Adams,A., Raffa,K., Adams,S., Book,A., Currie,C. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2011) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Streptomyces sp. SirexAA-E
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            environment                       :: Soil, Terrestrial
            num_replicons                     :: 1
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Free living
            trophic_level                     :: Missing
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: Missing
            sequencing_meth                   :: WGS
            assembly                          :: Newbler v. 2.3 (pre-release)
            finishing_strategy                :: Finished
            GOLD Stamp ID                     :: Gi05895
            Funding Program                   :: DOE-GLBRC 2008
            Source of Isolate :: Cameron Currie (currie@bact.wisc.edu)
            Cell Shape                        :: Filament-shaped
            Motility                          :: Nonmotile
            Sporulation                       :: Sporulating
            Temperature Range                 :: Mesophile
            Gram Staining                     :: Gram+
            Diseases                          :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Finished
            Current Finishing Status          :: Finished
            Assembly Method                   :: Newbler v. 2.3
            Genome Coverage                   :: 30x
            Sequencing Technology             :: 454/Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000177195.2-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 02:12:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,619
            CDSs (total)                      :: 6,532
            Genes (coding)                    :: 6,385
            CDSs (with protein)               :: 6,385
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 61 of 147
            Pseudo Genes (incomplete)         :: 120 of 147
            Pseudo Genes (internal stop)      :: 8 of 147
            Pseudo Genes (multiple problems)  :: 39 of 147
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:50:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 333102
            Orig. end                         :: 343633
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP002993.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4086644
            Source DNA and organism available from Cameron Currie
            (currie@bact.wisc.edu)
            Contacts: Cameron Currie (currie@bact.wisc.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10531
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="3"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10531
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10531
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5531](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5531)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            640..1803
                     /locus_tag="SACTE_RS01430"
                     /old_locus_tag="SACTE_0304"
     CDS             640..1803
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044241.1"
                     /locus_tag="SACTE_RS01430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0304"
                     /product="glycosyltransferase"
                     /protein_id="WP_014044241.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1e-18, bitscore:
                     66.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLHVITGLGVGGAEQQLRLMLRHLPTRCDVVTLTNPGAVAEGL
                     RSDGVRVRHLGMRGNRDLAALPRLARLVRQGGYDLVHTHLYRACVYGRIAASLAGTASV
                     ATEHSLGRDEIEGRPLTRGTRALYLRTERMGSATVAVSDTVAGRLRDWGVPAARIHTVP
                     NGIDAAAFRFDGRRRRAARALLGVPEDAFVVGGVGRLVPGKRFDVTLRAVAALPGAWLL
                     LAGDGPQAGPLRALAGRLGIADRVRFAGECGVDGAPDVPAALAAMDVFVSASREESFGL
                     AVVEALSAGLPVVHGPCPALDDLPAGQVPQGATRLRSPADPAAELTDVLRRHRAGGPHR
                     LPVPRAVDRYDIRLSGRRLMDVYAHVLGSPAPAPTHSTTPSTRRSHR"
     gene            1800..2483
                     /locus_tag="SACTE_RS01435"
                     /old_locus_tag="SACTE_0305"
     CDS             1800..2483
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044242.1"
                     /locus_tag="SACTE_RS01435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0305"
                     /product="LPS biosynthesis protein"
                     /protein_id="WP_014044242.1"
                     /transl_table=11
                     /translation="MTDSPDQQPSPGRLRGLRPSLPSFVRPSWWPLPACVLLGAACGLG
                     YGLLATPQYAATGYAVVTADKGVDPAAALGYAQSYGRLATSDATLAYAHGAAGESVRDL
                     RAHVRSETSPDSPMIAVTGTSSDPDRAADIANAVTEAVVAGAGHVAEDTGVKLVEFSHA
                     LAPADPVSPSAALGTAVGASAGGLVGGLVLLVRPRPGRRGVTAQVPGPAQAGGALHTGE
                     RELVG"
     gene            2480..3646
                     /locus_tag="SACTE_RS01440"
                     /old_locus_tag="SACTE_0306"
     CDS             2480..3646
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044243.1"
                     /locus_tag="SACTE_RS01440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0306"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_014044243.1"
                     /transl_table=11
                     /translation="MTPERHGRVRVTLCRDPREFSALAGEWDALHRRCPTATPFQSHAW
                     LHSWWLSYGVPGRLRVVLARRDGRLVGAAPLMLVHRPMPLLVPLGGAISDFFDVLVDAE
                     RTGEAVAALERGLHRAAAHSVVDLREVRPGASAQSLFAAWSGARSRLTDSTCMELPAEP
                     VGALIGRMPGSRAQRARAKLRRIDALGIECRALPEDRVADGVRTLLRLHELQWRGRGVN
                     PEHLRPRFRAHLVRSVGRMVRDGEAAVREFLLDGEVVAADLALGSAGLTGGYLYGVDPR
                     LRERKVDVSTMLLRELARQAADSGGGVLSLLRGSEQYKGHWGPEPVVNQRLLLARSSLE
                     PLLRLRESQVVVRDRAARTVKTRFPAARDWRERLGALPSAKPSATVRR"
     misc_feature    3746..3761
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.09"
                     /tool="antismash"
     gene            3757..4038
                     /locus_tag="SACTE_RS01445"
                     /old_locus_tag="SACTE_0307"
     CDS             3757..4038
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575906.1"
                     /locus_tag="SACTE_RS01445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0307"
                     /product="chaplin"
                     /protein_id="WP_014044244.1"
                     /transl_table=11
                     /translation="MSRISKAAVVMAGTGALIAGGAGFASADAGAEAVAAHSPGVLSGN
                     AVQVPVHVPVNVCGNTVNVIALLNPVFGNSCANVSDDHEDEGDYGYGY"
     gene            complement(4122..4982)
                     /locus_tag="SACTE_RS01450"
                     /old_locus_tag="SACTE_0308"
     CDS             complement(4122..4982)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157951.1"
                     /locus_tag="SACTE_RS01450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0308"
                     /product="tyrosinase family protein"
                     /protein_id="WP_014044245.1"
                     /transl_table=11
                     /translation="MYSRKNQRNLTRTEKKRLVGAILELKRSGRYDEFVSLHRQYYVTD
                     AESRPRPAHMTPSFFPWHRRYLLEFEKALQTVDSGVTVPYWDWTQDNTPAASLWAEDFL
                     GGNGRAGDRQVVTGPFAYAEGNWRIRAGVTDDRFLRRDFGRPSAPVTLPTKAEVDRALG
                     DPVYDAAPWNSLSTTGFRNRIEGWGIRGVRGVGNHNRVHRWIGGSMAGAASPDDPVFWL
                     HHAYIDLLWTRWQKAHPRARYLPDRAPAAGDPQRGRIFALDDPMPPWDAKPSQLLDHSR
                     LYRYV"
     gene            complement(5001..5531)
                     /locus_tag="SACTE_RS01455"
                     /old_locus_tag="SACTE_0309"
     CDS             complement(5001..5531)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044246.1"
                     /locus_tag="SACTE_RS01455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0309"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_014044246.1"
                     /sec_met_domain="melC (E-value: 1.7e-49, bitscore: 166.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPTTATDGTTATAVPPADTPHSPDATPAGAPGRHRPARRAVLRT
                     AFTAAVVAGTGGVLAPVLFKDPGTPGRGERRSQAPERFSERYRGRDIRGGVSGLVPAGG
                     QARPGEAAAASTATVAEVTIDGRPLHVMRRADGSYLSEVNHYESFPTLLETARAAVDEL
                     GTAQLSATTPHTI"
     misc_feature    5571..5587
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            5725..7023
                     /locus_tag="SACTE_RS01460"
                     /old_locus_tag="SACTE_0310"
     CDS             5725..7023
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044247.1"
                     /locus_tag="SACTE_RS01460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0310"
                     /product="cytochrome P450"
                     /protein_id="WP_014044247.1"
                     /sec_met_domain="p450 (E-value: 9.7e-17, bitscore: 59.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAPPQVSTDRVRGAGPASLPDLADPSFWRLPRADRLAGFARLRE
                     HGEPVLFTPRTGAHQPFRALVGHADVRAASRQPRVFASAPGVTTPEPARWAKAVFGNSM
                     VHMDGPEHAGLRRTISRAFTPRLLTAAEENIAGTARRLVDEVVAGRPEDFMRSAAARLP
                     FEVICDLMGVPVRYRPEIAAQIDHASEYVGVQRRGRARLRVPGRGLRSLARMQLVMARL
                     AAERRRRPAEDLVSALVRADVDGRSLSARELGAFFSLLLVAGVETTRNALAHGLHLLTV
                     HPEQRALLESDFERYADGAVDEIVRHSTPIIQFRRTVREAHELGGRVFVPGEKVMLLYA
                     SANRDASVFTAPDVFDITRSPNPHLGYGGGGPHHCLGAHLARLEMKALYRELLGRPVPV
                     RAAGDPVLVASNFDHRVRSLPLSYGLFHPNEPV"
     misc_feature    complement(7100..7114)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.37"
                     /tool="antismash"
     gene            7133..8335
                     /locus_tag="SACTE_RS01465"
                     /old_locus_tag="SACTE_0311"
     CDS             7133..8335
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044248.1"
                     /locus_tag="SACTE_RS01465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0311"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_014044248.1"
                     /transl_table=11
                     /translation="MPAQRRLINFGIGTAVLGLLVGGVLVADRNGADSAAGKGADASLP
                     PVAQGPTAVGAYLDYGPQGVRRMAELSRWLGGTELRVGHSYLPGDVWENIEGRPGFLGS
                     WAAWRKAAPDRMFVLNTPMLERNEDHVPDDEVRSLLRRGAGGEFDAHFRKLAERLVLLG
                     VPDTVLVLGWEMNGTTYTHRCGPDPAAWKAYWTRIVTAMRSVPGQEFRFDFAPSRGRDA
                     VPWTECYPGDDVVDIIGMDSYDQPPARTFEEQVNEPYGLRKQVDFAAEHGKPISFPEWG
                     LFRNGDNPRYMRGMLEWMERHKPVYQTITDYCPHGVWQCAENPESSRVYREMLSAGPDD
                     GGPSAPAPTPTPETTRTSEAPVPDEPEPETGTEAPGWCVSLPLSDLFGPWARDHEFCAG
                     S"
     gene            complement(8475..10292)
                     /locus_tag="SACTE_RS01470"
                     /old_locus_tag="SACTE_0312"
     CDS             complement(8475..10292)
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044249.1"
                     /locus_tag="SACTE_RS01470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SACTE_0312"
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="WP_014044249.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 6.7e-45, bitscore:
                     151.3, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.3e-27, bitscore:
                     95.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVSDYVLQRLREWEVDHVFSYAGDGINGLLAAWGRAENKPKFVQ
                     ARHEEMAAFEAVGYAKFSGKVGVCAATSGPGAIHLLNGLYDAKLDHVPVVAIVGQTNRS
                     AMGGSYQQEVDLLSLYKDVASDFCEMVTVPEQLPNVIDRAIRTAYGRRTVTAVIIPADV
                     QELDYSPPTHAFKMVPSSLGLSRSVPVPADDELDRAAEVLNAGEKVAVLIGQGARGARA
                     EVEAVADLLGAGVAKALLGKDVLPDDLPYVTGSIGLLGTRPSYELMTDCDTLLVVGSNF
                     PYAQFLPEFDQARAVQIDIDPSMLGLRYPFEVNLAGDAQATLAALLPRLKRKKHGAWRK
                     KIEKDTARWWDVMERRAAVEADPINPEHVVHSLDSLLPDDVILSADSGSAANWYARHLR
                     IRGSMRGSLSGTLATMGPGVPYAIGAKFAHPGRPALAIVGDGAMQMNGMAELITAAKYW
                     QEWEDPRLIVAVLNNQDLNQVTWEMRAMSGAPQFLPSQAIPDVPYADFARSVGLGGVRV
                     EKPDQVEAAWRQALAADRPFVIDFRTDPAVPPIPPHATREQAEAVASAIVKGDSDRGGM
                     VKQGIKAKMQEFLPGHRHSDKESGPEQDR"
ORIGIN
        1 cgaccgcctg gacgagcagc tgacgtggct gcgccgccgg ggcctgaccg gggtgggcat
       61 cgcgtcgctg ctgcgcgcgg acgcgtccga gcggcgcggg ctggtcgggc tgacgttcga
      121 cgacgggtac gccgacttcg tccaggaggc gcttccggtg ctcctcggac acggctgcac
      181 ggcgaccgtg ttcgtcctgc ccggccggct cggcgggtcc aacgactggg atccgctggg
      241 cccgcgccgg ccgctgctca ccgaggaggg catccggacc gtcgcggcgg ccggtatgga
      301 gatcggctcg cacggcatgg tccacctgga cctgacggcc ctgtccgacg ccgcgctgcg
      361 ccgcgagacg cacggcagcc gggaggccct cggccgcgtc accggggtcc cgccggccgg
      421 tttctgctat ccgtacggca gggccgacgc acgggtgctc gcctcggtgc gggacgccgg
      481 gtacgcgttc ggctgcgccc tggaccccgg cccgttgcgc gggccgctcg ccctgccgcg
      541 cacccacgtc agccacgccg accggtccac ccgcctccgc gcgaaggcgc tgcgccaccg
      601 gctgcggcac cggtcggcgg acgtatccgg ggggcaccgg tgaaggtgct gcacgtcatc
      661 accggtctcg gcgtcggcgg ggccgagcag cagctgcggc tgatgctgcg tcacctgccc
      721 acccgttgcg acgtcgtcac gctcaccaac ccgggggcgg tcgccgaggg actgcgctcg
      781 gacggggtgc gggtgcggca cctgggcatg cggggcaacc gggacctggc cgctctgccg
      841 aggctcgccc ggctcgtgcg gcagggcggg tacgacctcg tacacacgca tctgtaccgg
      901 gcgtgcgtct acggacggat cgcggcctcg ctcgccggta ccgcctcggt ggccacggag
      961 cactcgctgg gccgcgacga gatcgaggga cgcccgctca cccgggggac ccgcgcgctg
     1021 tatctgcgga ccgagcggat gggctcggcg acggtcgccg tctccgacac ggtcgccgga
     1081 cggctgcggg actggggggt gccggccgcc cggatccaca cggtgcccaa cggcatcgac
     1141 gcggcggcgt tccgcttcga cggccggcgg cggcgggcgg cccgggcact gctcggcgtt
     1201 cccgaggacg ccttcgtggt gggcggtgtg gggcgtctgg tgcccggcaa acgcttcgac
     1261 gtgacgctgc gggccgtcgc ggcgctgccc ggcgcctggc tcctgctggc cggcgacggc
     1321 ccgcaggccg gaccgctgcg cgccctcgcc ggccggctgg ggatcgccga ccgggtccgg
     1381 ttcgccgggg agtgcggcgt ggacggcgcg ccggacgtcc cggcggcgct cgcggccatg
     1441 gacgtcttcg tctcggcgtc ccgggaggag tccttcgggc tcgccgtcgt cgaggcgctg
     1501 tccgcggggc tgcccgtcgt gcacggcccc tgtcccgccc tcgacgacct gccggccggt
     1561 caggtccccc agggcgccac ccgtctgcgg tccccggccg accccgccgc cgagctgacg
     1621 gacgtgctgc ggcgccacag ggcgggcggg ccgcacaggc tgccggtgcc ccgggcggtg
     1681 gaccgctacg acatccggct cagcggccgc cggctcatgg acgtgtacgc gcacgtcctc
     1741 ggctcccccg cacccgcccc gacccactcg accaccccct ctacccggag gtcacaccga
     1801 tgaccgactc ccccgaccag cagccctccc ccggacggct gcgcgggctc cgcccctcct
     1861 tgccctcctt cgtacggccc agctggtggc cgctgcccgc ctgtgtcctg ctcggggcgg
     1921 cctgcgggct cgggtacggg ctgctcgcca ccccgcagta cgccgcgacc gggtacgccg
     1981 tggtgacggc cgacaagggc gtcgaccccg cggcggcgct cgggtacgcc cagtcgtacg
     2041 ggcggctggc gaccagcgac gcgacgctcg cctacgccca cggcgccgcc ggcgagtccg
     2101 tgcgtgacct ccgcgcccac gtccggtccg agacctcccc ggactcgccg atgatcgcgg
     2161 tcaccggcac gtcgtccgac ccggaccggg ccgcggacat cgccaacgcg gtcaccgagg
     2221 ccgtggtggc cggtgcgggt cacgtcgccg aggacaccgg cgtgaagctg gtcgagttct
     2281 cgcacgccct cgcgccggcc gatccggtct ccccctccgc ggcactcggc acggcggtgg
     2341 gcgcgagcgc cggggggctg gtcggcgggc tggtcctgct cgtacggccg cgtccggggc
     2401 gccggggcgt caccgcccag gtcccggggc cggcccaggc gggcggcgcg ctgcacaccg
     2461 gggaacggga gctcgtggga tgaccccgga gcgtcacggc cgcgtccgtg tgaccctgtg
     2521 ccgggatccg cgggagttct ccgcgctcgc cggtgagtgg gacgcgctgc accgccggtg
     2581 cccgaccgca acgccgttcc agagccacgc ctggctccac tcctggtggc tgtcgtacgg
     2641 ggttccggga cggctcaggg tcgtcctcgc gcgccgggac ggccgtctcg tcggcgccgc
     2701 cccgctgatg ctcgtccacc ggccaatgcc gttgctcgtg ccgctggggg gtgcgatctc
     2761 cgacttcttc gacgtcctcg tcgacgccga gcggaccggg gaggcggtcg ccgccctgga
     2821 gcgcgggctg caccgggcgg ccgcgcacag cgtggtggac ctgagggagg tacggcccgg
     2881 cgcctcggcc cagtcgctgt tcgcggcatg gtccggtgcc cgcagccggc tgaccgactc
     2941 cacctgcatg gagctgcccg ccgagccggt cggcgcgctc atcggccgga tgccgggctc
     3001 acgcgcccag cgggcccggg ccaagctgcg gcggatcgac gccctgggca tcgagtgccg
     3061 cgccctgccc gaggaccggg tggccgacgg ggtgcgcacc ctgctgcggc tgcacgagct
     3121 ccagtggcgg ggccggggcg tcaaccccga gcacctgcgc ccccggttcc gcgcccatct
     3181 ggtgcgctcc gtggggcgga tggtacgcga cggcgaggcg gcggtccggg agttcctgct
     3241 cgacggggag gtggtggcgg ccgatctggc cctcggttcc gccggtctca ccggcggcta
     3301 tctctacggg gtggacccgc gcctgaggga acgcaaggtc gatgtgtcga cgatgctgct
     3361 gcgggaactg gcccggcagg cggcggactc gggcggcggc gtgctgagcc tgctgcgcgg
     3421 ctccgagcag tacaagggcc attggggccc cgagccggtg gtcaaccagc gtctgctgct
     3481 ggcgcgttcc tccctggagc cgttgctgag gctgcgcgag tcccaggtgg tggtgcggga
     3541 ccgggcggcg cggaccgtga agacgcgttt cccggccgcg cgtgactggc gcgagcggct
     3601 gggcgccctc ccgtcggcga agccgtcggc caccgtccgg agatgatcct ggattacgac
     3661 ttttgctccg atccgtgacc gtctgtcgac ttccgcgttg tcagggcatg ccgggcacgt
     3721 tctgtggccg gttccgattc cttccaagga gaattgatgt cccgcatctc gaaggcagcc
     3781 gttgtcatgg cgggtaccgg cgcgctgatc gccggtggcg ccggcttcgc ctccgccgac
     3841 gccggcgccg aggccgtcgc cgcccactcc cccggtgtcc tctcgggcaa cgccgtccag
     3901 gtgccggtgc acgtcccggt caacgtctgc ggcaacaccg tcaacgtcat cgcgctgctg
     3961 aacccggtct tcggcaactc ctgcgcgaac gtctccgacg accacgagga cgagggcgac
     4021 tacggctacg gctactgagc cccgcagctg acgcaccccg ggccttccct ccccgctcag
     4081 gcggggcggg aaggcccggt cccgttccgg agccaggtgg ttcagacgta ccggtagagg
     4141 cggctgtggt ccagcagctg cgagggcttc gcgtcccagg gcggcatcgg gtcgtccagg
     4201 gcgaagatcc ggccccgttg cgggtcgcct gccgcgggag cgcggtcggg caggtagcgg
     4261 gctcgggggt gcgccttctg ccagcgggtc cagagcaggt cgatgtaggc gtggtgcagc
     4321 cagaagaccg ggtcgtcggg cgacgcggca ccggccatcg aaccgccgat ccagcggtgg
     4381 acccggttgt ggttgccgac gccccgtacg ccccggatgc cccagccctc gatcctgttg
     4441 cggaacccgg tggtgctgag gctgttccag ggcgcggcgt cgtagacggg gtcgccgagg
     4501 gcccggtcca cctcggcctt cgtgggcagg gtgaccggtg ccgaggggcg gccgaagtcg
     4561 cgccgcagga agcggtcgtc ggtgacgccc gcgcggatcc tccagttgcc ctccgcgtac
     4621 gcgaacggcc cggtcacgac ctggcggtcg cccgcccggc cgttgccgcc gaggaagtcc
     4681 tccgcccaga gggaggctgc cggggtgttg tcctgggtcc agtcccagta cgggacggtc
     4741 accccggagt cgacggtctg cagggccttc tcgaactcca ggaggtatct gcggtgccac
     4801 gggaagaagg acggggtcat gtgagcgggc ctcggccggc tctccgcgtc ggtgacgtag
     4861 tactgccggt gcagcgagac gaactcgtcg taccggcccg accgcttgag ttccaggatg
     4921 gcgccgacga ggcgcttctt ctcggtgcgt gtgaggttgc gctgattctt gcggctgtac
     4981 acccgtgctc gtccttcgcc tcagatggtg tggggggtgg tggcggacag ctgtgcggtg
     5041 ccgagctcgt cgaccgcggc gcgggccgtt tcgaggagcg tcgggaagga ctcgtagtgg
     5101 ttgacctcgc tgaggtagct gccgtcggcg cgccgcatca cgtgcagcgg ccggccgtcg
     5161 atcgtgacct cggcgaccgt cgcggtggac gccgccgcgg cctcgccggg ccgtgcctgc
     5221 cctccggcgg gcaccaggcc ggagacgccg ccccggatgt cccggccccg gtacctctcg
     5281 gagaagcgtt cgggggcctg ggatctgcgc tccccgcgcc cgggggtgcc ggggtccttg
     5341 aagaggaccg gagccagtac cccgccggta ccggcgacga cggccgcggt gaaggcggtc
     5401 cgcagtacgg cgcggcgggc ggggcggtgc cttccggggg cgcccgccgg ggtggcgtca
     5461 gggctgtggg gggtgtccgc cggcgggacg gccgtggcgg tcgtgccgtc cgtcgctgtg
     5521 gtcgggtcca tgaagattcc tgtctgtcgt ccactctcgt gtgtggtgcg tgctctcgag
     5581 gtgggcaacg agacggcgga cgaacggttc tcgtcctgtg cgcgtcacgc gaccgtggga
     5641 attccgcgcc gacggcggcc acgtcggccg tccggcggaa cgcttcgggc cagcatggcg
     5701 gtccgtacgt ccgggaggct gcccatgaac gcaccgccgc aggtgtccac cgaccgtgtg
     5761 cggggagccg gcccggccag tctgcccgac ctggccgatc cgtcgttctg gcggttgccg
     5821 agggccgatc ggctggcggg cttcgcccgg ctgcgggagc acggcgagcc ggtgctcttc
     5881 accccgcgca cgggcgcgca ccaaccgttc cgggccctgg tcgggcacgc cgacgtgagg
     5941 gcggcgagcc ggcagccgcg cgtcttcgcc agtgcgccgg gggtcaccac gcccgaaccg
     6001 gcgcgctggg cgaaggcggt gttcggcaac tccatggtgc acatggacgg ccccgaacac
     6061 gccggtctgc gcaggaccat ctcgcgggcc ttcacgcccc ggctgctcac ggcggccgag
     6121 gagaacatcg ccgggaccgc gcggcggctg gtggacgagg tggtcgcggg acggcctgag
     6181 gatttcatgc gttcggccgc cgcccgtctg ccgttcgagg tgatctgcga cctgatgggc
     6241 gtcccggtcc ggtaccgccc cgagatcgcc gcgcagatcg accacgcgtc cgagtacgtc
     6301 ggggtgcagc gccggggccg ggcccggctg cgggtgccgg ggcgcgggct gcggtcgctg
     6361 gcccggatgc agctcgtgat ggcccggctg gcagcagagc ggcgccgacg cccggccgag
     6421 gacctggtct cggcgctggt gcgggcggac gtcgacgggc ggtcgctgtc cgcccgcgaa
     6481 ctgggggcgt tcttctcgct gctgctcgtg gccggtgtgg agaccacccg taacgcgctg
     6541 gcgcacgggc tccacctgct gaccgtccat ccggagcagc gagccctgct ggagtcggac
     6601 ttcgagcggt acgcggacgg cgcggtggac gagatcgtgc gccattcgac gccgatcatc
     6661 cagttccgcc ggacggtgcg tgaggcacac gagctcggtg gccgcgtctt cgtccccggc
     6721 gagaaggtga tgctcctcta cgcctcggcg aaccgggacg cgtccgtctt caccgccccc
     6781 gacgtcttcg acatcacccg gtccccgaat ccgcacctgg ggtacggcgg cggcggcccg
     6841 caccactgtc tgggcgcgca tctggcgcgt ctggagatga aggccctcta ccgcgaactg
     6901 ctcggccgcc cggtaccggt gcgggcggcc ggcgacccgg tgctggtcgc ctcgaacttc
     6961 gaccaccgcg tccgttcgct cccgctgtcc tacggcctct ttcacccgaa cgagccggtc
     7021 tgatcctccg cacgccacga atgccgtgcc cggcggacgt cgtgcagcac atcaccgggc
     7081 ctttctccat agggtcggca gggccggggg gcccacgttt cgaggaggag ccgtgcccgc
     7141 acaacgccgt ctcatcaact tcggtatcgg gacggctgtg ctcggccttc tcgtcggtgg
     7201 cgtgctggtc gccgaccgga acggcgcgga ctccgcggcg ggcaagggcg ccgacgcgtc
     7261 cttgccaccg gtcgcccagg gcccgaccgc cgtcggcgcc tatctggact acgggcccca
     7321 gggcgtacgg cggatggcgg agctctcgcg ctggctgggc gggacggagc tgcgcgtggg
     7381 gcacagctac ctgccgggag acgtgtggga gaacatcgag ggccgccccg gtttcctcgg
     7441 ttcgtgggcg gcctggcgca aggccgcgcc agaccggatg ttcgtcctca acacgccgat
     7501 gctggagcgg aacgaggacc acgtcccgga cgacgaggtg cgctccctgc tccggcgggg
     7561 tgccggcggg gagttcgacg cgcacttccg caagctcgcc gagcggctgg tcctgctggg
     7621 tgtcccggac acggtgctcg tcctcggctg ggagatgaac ggcaccacgt acacccaccg
     7681 ttgcggtccc gacccggccg cgtggaaggc gtactggaca cgcatcgtca ccgcgatgcg
     7741 ctcggtcccc ggccaggagt tccgcttcga cttcgccccg agccgcggcc gggacgccgt
     7801 gccgtggacg gagtgctatc ccggtgacga cgtcgtggac atcatcggca tggactccta
     7861 cgaccagccg ccggcccgta ccttcgagga gcaggtgaac gagccgtacg ggctgcggaa
     7921 gcaggtggac ttcgcggcgg agcacggcaa gccgatctcg ttcccggagt ggggcctgtt
     7981 ccgcaacggc gacaacccgc ggtacatgcg cggcatgctg gagtggatgg agcggcacaa
     8041 gccggtgtac cagaccatca cggactactg tccgcacggt gtgtggcagt gcgcggagaa
     8101 ccccgagtcc tcccgcgtct accgcgagat gctctccgcc ggtccggacg acggcgggcc
     8161 gtccgccccc gccccgaccc cgaccccgga gaccacacgg acgtccgagg cccccgttcc
     8221 ggacgagccg gaaccggaga cggggaccga ggcccccggg tggtgcgtca gtctcccgtt
     8281 gagtgatctg ttcgggccgt gggcgcggga ccacgagttc tgcgcgggct cctgagaggc
     8341 gtggcgcccc ctcgtcaccg cccggtgcgg cggccggcgg ccatggggtg atgccgccgc
     8401 accgggtggg gcgtccgcct ccgcgagcga ggtacgcgcc gagccggacg gcctccacca
     8461 gggggacgga caggtcaccg gtcctgttcg ggaccggatt ccttgtcgga gtgccggtgg
     8521 ccgggaagga actcctgcat cttcgccttg atgccctgtt tgaccatgcc tccgcggtcg
     8581 ctgtcgccct tcacgatggc ggacgccacc gcttcggcct gctcccgggt ggcgtgcggc
     8641 gggatgggcg ggacggccgg gtcggtgcgg aagtcgatca cgaaggggcg gtcggcggcg
     8701 agcgcctggc gccatgccgc ttccacctgg tccggcttct cgacccgtac gccgccgagc
     8761 ccgacggacc gcgcgaagtc ggcgtacggg acgtccggaa tggcctggga cggcaggaac
     8821 tgcggggcgc ccgacatggc acgcatctcc caggtgacct ggttgaggtc ctggttgttc
     8881 agcacggcga cgatgagccg cgggtcctcc cactcctgcc agtacttggc ggcggtgatc
     8941 agttcggcca tgccgttcat ctgcatggcg ccgtccccga cgatcgcgag ggccgggcgg
     9001 ccggggtggg cgaacttggc gccgatggcg tacggcacgc cggggcccat cgtggcgagt
     9061 gtgccggaca gggagccgcg catcgagccg cggatgcgca ggtggcgggc gtaccagttg
     9121 gcggcggagc cggagtcggc ggacaggatg acgtcgtccg gcaggagcga gtcgaggctg
     9181 tgcacgacgt gctccgggtt gatgggatcg gcctcgacgg cggcgcggcg ttccatgacg
     9241 tcccaccagc gggcggtgtc cttctcgatc ttcttgcgcc aggccccgtg cttcttccgc
     9301 ttcagccgtg gcagcagggc cgccagcgtc gcctgcgcgt cgccggccag gttgacctcg
     9361 aacgggtagc gcagcccgag catggacggg tcgatgtcga tctggaccgc gcgcgcctgg
     9421 tcgaactccg gcaggaactg cgcgtagggg aagttggatc ccaccaccag cagcgtgtcg
     9481 cagtccgtca tgagttcgta ggaggggcgg gtgcccagca ggccgatcga tccggtgacg
     9541 tacggcaggt cgtcggggag gacgtccttg ccgagcagtg ccttggcgac gccggcgccc
     9601 agcaggtcgg ccaccgcctc gacctcggcc cgggcgccgc gcgcgccctg cccgatcagg
     9661 acggcgacct tctcgcccgc gttcagcacc tcggcggccc ggtccagctc gtcgtccgcc
     9721 ggtacgggta cggagcggga caggccgagg ctggagggca ccatcttgaa ggcgtgcgtg
     9781 ggcggcgagt agtcgagttc ctgcacgtcc gcggggatga tgacggccgt caccgtccgc
     9841 cttccgtagg cggtgcggat ggcccggtcg atgacgttgg gcagctgttc ggggacggtg
     9901 accatctcgc agaagtcgga ggccacgtcc ttgtacaggc tcagcaggtc gacctcctgc
     9961 tggtaggagc cgcccatggc gctgcgattg gtctgcccga cgatggccac gacggggacg
    10021 tggtccagct tcgcgtcgta gaggccgttg agcaggtgga tggcgcccgg gccggacgtc
    10081 gccgcgcaga ccccgacctt gccggagaac ttggcgtagc ccacggcctc gaaggcggcc
    10141 atctcctcgt gccgggcctg gacgaacttc ggcttgttct cggcccgtcc ccaggcggcc
    10201 agcaggccgt tgatgccgtc acccgcgtag gagaagacgt ggtccacctc ccactcgcgc
    10261 agccgctgca gtacgtagtc ggacaccttc atcgacacag ttctccgctt tctctctcag
    10321 gtaggggcgg gcgccccgca ccgtcggtcg gttgctcggc gccgagaccg atcggcacct
    10381 gccggtagct cgccccgtcg gtggagcggg cgccccggcg gccggcccca cggctcaccc
    10441 cgcttccgcg gcggatacgc agtcggactt catacaggta caaaacccca tggcagtccc
    10501 acgggcatcc tgaccgccgc ctgacccgtg a
//
