GCF_000159635.1 - 7 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_GG667198.1 (Corynebacterium lipophiloflavum DSM 44291)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	ectoine	7,835	18,209

NZ_GG667196.1 (Corynebacterium lipophiloflavum DSM 44291)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 14.1	terpene	35,398	58,363	Medium	carotenoid	terpene

NZ_GG667193.1 (Corynebacterium lipophiloflavum DSM 44291)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 17.1	T1PKS	49,287	94,059
Region 17.2	NAPAA	185,502	219,278

NZ_GG667192.1 (Corynebacterium lipophiloflavum DSM 44291)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 18.1	terpene-precursor	281,819	302,913

NZ_GG667191.1 (Corynebacterium lipophiloflavum DSM 44291)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 19.1	lanthipeptide-class-i	83,558	110,131	Low	geobacillin I	ribosomal:RiPP:Lanthipeptide
Region 19.2	terpene	372,533	393,549

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	ectoine	7,835	18,209
Region 14.1	terpene	35,398	58,363	Medium	carotenoid	terpene
Region 17.1	T1PKS	49,287	94,059
Region 17.2	NAPAA	185,502	219,278
Region 18.1	terpene-precursor	281,819	302,913
Region 19.1	lanthipeptide-class-i	83,558	110,131	Low	geobacillin I	ribosomal:RiPP:Lanthipeptide
Region 19.2	terpene	372,533	393,549

No secondary metabolite regions were found in these records:: NZ_GG667209.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667208.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667207.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667206.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667205.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667204.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667203.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667202.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667201.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667200.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667199.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667197.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667195.1 (Corynebacterium lipophiloflavum DSM 44291); NZ_GG667194.1 (Corynebacterium lipophiloflavum DSM 44291)

Compact view

NZ_GG667198.1 - Region 1 - ectoine

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Location: 7,835 - 18,209 nt. (total: 10,375 nt) Show pHMM detection rules used

Region on contig edge.

ectoine: ectoine_synt

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

HMPREF0298_RS10550: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

Transferases: phosphorylase

HMPREF0298_RS10575: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000855.3	0.49	other (other)	ectoine	Methylomicrobium kenyense
BGC0000859.3	0.49	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000857.3	0.45	other (other)	ectoine	Methylophaga alcalica
BGC0000854.3	0.42	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0001778.5	0.35	other (other)	showdomycin	Streptomyces showdoensis
BGC0000913.3	0.26	other (other)	MK-8	Enterobacter cloacae
BGC0002042.3	0.25	NRPS	anthelvencin A, anthelvencin B, anthelvencin C	Streptomyces venezuelae
BGC0002097.3	0.21	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0001073.5	0.21	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0000029.5	0.19	PKS	BE-14106	Streptomyces sp. DSM 21069

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000855.3	0.49	other (other)	ectoine	Methylomicrobium kenyense
BGC0000859.3	0.49	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000857.3	0.45	other (other)	ectoine	Methylophaga alcalica
BGC0000854.3	0.42	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0001778.5	0.35	other (other)	showdomycin	Streptomyces showdoensis
BGC0000913.3	0.26	other (other)	MK-8	Enterobacter cloacae
BGC0002042.3	0.25	NRPS	anthelvencin A, anthelvencin B, anthelvencin C	Streptomyces venezuelae
BGC0002097.3	0.21	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0001073.5	0.21	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0000029.5	0.19	PKS	BE-14106	Streptomyces sp. DSM 21069

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

Additional weak hits (2):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.42 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_GG667196.1 - Region 1 - terpene

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Location: 35,398 - 58,363 nt. (total: 22,966 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

HMPREF0298_RS09675: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

HMPREF0298_RS09690: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: acetyltransferase

HMPREF0298_RS09635: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000637.3	0.65	terpene	carotenoid	Corynebacterium glutamicum
BGC0000644.3	0.63	terpene	carotenoid	Dietzia sp. CQ4
BGC0000656.5	0.50	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000650.3	0.50	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000636.3	0.47	terpene	carotenoid	Brevibacterium linens
BGC0000648.3	0.46	terpene	carotenoid	Myxococcus xanthus
BGC0001862.4	0.44	ribosomal	pallidocin	Aeribacillus pallidus
BGC0000643.3	0.44	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.43	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000551.5	0.41	ribosomal	SapB	Streptomyces coelicolor A3(2)

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000637.3	0.65	terpene	carotenoid	Corynebacterium glutamicum
BGC0000644.3	0.63	terpene	carotenoid	Dietzia sp. CQ4
BGC0000656.5	0.50	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000650.3	0.50	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000636.3	0.47	terpene	carotenoid	Brevibacterium linens
BGC0000648.3	0.46	terpene	carotenoid	Myxococcus xanthus
BGC0001862.4	0.44	ribosomal	pallidocin	Aeribacillus pallidus
BGC0000643.3	0.44	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.43	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000551.5	0.41	ribosomal	SapB	Streptomyces coelicolor A3(2)

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

HMPREF0298_RS09685: PT_phytoene_like

Domain 1 (20 - 281): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → diapophytoene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

HMPREF0298_RS09695: Lycopene_cycl

Domain 1 (4 - 92): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

HMPREF0298_RS09700: Lycopene_cycl

Domain 1 (1 - 90): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.12 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.11 out of 31.73

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_GG667193.1 - Region 1 - T1PKS

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Location: 49,287 - 94,059 nt. (total: 44,773 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

HMPREF0298_RS06985: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase glycosyltransferase methyltransferase transferase

HMPREF0298_RS07015: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

HMPREF0298_RS07050: methyltransferase

smcogs: SMCOG1089: methyltransferase

HMPREF0298_RS07055: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

HMPREF0298_RS07090: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Hydrolases: esterase

HMPREF0298_RS06980: putative esterase

smcogs: SMCOG1104: putative esterase

HMPREF0298_RS06990: putative esterase

smcogs: SMCOG1104: putative esterase

Ligases: ligase synthase

HMPREF0298_RS07005: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

HMPREF0298_RS07010: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transport

HMPREF0298_RS07030: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000107.3	0.63	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001258.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000107.3	0.63	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001258.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.26 out of 19.26

Confidence: strong

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.08 out of 21.55

Confidence: medium

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 22.40 out of 22.40

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 17.80 out of 18.16

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

Additional weak hits (9):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.12 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.53 out of 20.97

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.35 out of 27.55

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.95 out of 25.88

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.24 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.06 out of 21.55

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.47 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 49286 - 94059:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

HMPREF0298_RS07005: Acetyl-CoA

CAL_domain (65..514): Acetyl-CoA

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 26.6
AHBA: 17.1
shikimic_acid: 15.6
NH2: 6.2
fatty_acid: 0.0

HMPREF0298_RS07010: pk

PKS_AT (635..929): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: fatty_acid

Prediction, score:

fatty_acid: 109.4
Methylmalonyl-CoA: 71.9
Methoxymalonyl-CoA: 70.3
Malonyl-CoA: 60.7
Propionyl-CoA: 49.0
Ethylmalonyl-CoA: 48.3
2-Methylbutyryl-CoA: 38.7
trans-1,2-CPDA: 33.4
Isobutyryl-CoA: 24.4
CHC-CoA: 24.4
Acetyl-CoA: 22.7
inactive: 20.4
Benzoyl-CoA: 11.7
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
ArcA	Repressor of carbon oxidation
CRP	Cyclic AMP receptor protein
HypR	L-hydroxyproline utilization repressor

NZ_GG667193.1 - Region 2 - NAPAA

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Location: 185,502 - 219,278 nt. (total: 33,777 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: deacetylase

HMPREF0298_RS07510: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Lyases: hydratase

HMPREF0298_RS07545: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase

HMPREF0298_RS07560: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.76	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.59	NRPS	epichloenin A	Epichloe festucae
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002171.2	0.45	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.45	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001873.3	0.44	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0000889.5	0.42	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.76	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.59	NRPS	epichloenin A	Epichloe festucae
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002171.2	0.45	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.45	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001873.3	0.44	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0000889.5	0.42	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 22.40 out of 22.40

Confidence: strong

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 22.40 out of 22.40

Confidence: strong

Additional weak hits (2):

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 21.84 out of 32.38

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.50 out of 25.75

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 185501 - 219278:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

HMPREF0298_RS07560: X

AMP-binding (21..383): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIVGGEWNTYGPTESTVVASA
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (68% 8Å match); Gln or Tyr DAESVGTVVK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
HypR	L-hydroxyproline utilization repressor

NZ_GG667192.1 - Region 1 - terpene-precursor

Download region GenBank file

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Location: 281,819 - 302,913 nt. (total: 21,095 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase glycosyltransferase

HMPREF0298_RS04505: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

HMPREF0298_RS04515: acyltransferase

smcogs: SMCOG1141: acyltransferase

Ligases: synthetase

HMPREF0298_RS04550: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000936.5	0.37	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0001456.3	0.37	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.36	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001521.3	0.36	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000664.5	0.36	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.35	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.35	terpene	isorenieratene	Streptomyces collinus Tu 365

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000936.5	0.37	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0001456.3	0.37	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.36	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001521.3	0.36	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000664.5	0.36	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.35	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.35	terpene	isorenieratene	Streptomyces collinus Tu 365

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

HMPREF0298_RS04550: PT_FPPS_like

Domain 1 (51 - 362): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.10 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.08 out of 23.79

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.84 out of 24.78

Confidence: strong

Additional weak hits (13):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.78 out of 39.62

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.89 out of 33.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.77 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.11 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.85 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.75 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.67 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.51 out of 24.78

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.04 out of 39.62

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.41 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.46 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.43 out of 33.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.61 out of 20.01

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
LexA variant 2	Repressor of DNA damage response

NZ_GG667191.1 - Region 1 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 83,558 - 110,131 nt. (total: 26,574 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

HMPREF0298_RS00475: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Lyases: cyclase dehydratase

HMPREF0298_RS00430: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

HMPREF0298_RS00435: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

HMPREF0298_RS00440: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.53	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.50	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002346.3	0.49	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000506.4	0.49	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000542.3	0.49	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000556.5	0.49	ribosomal	streptin	Streptococcus pyogenes
BGC0000559.4	0.49	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002316.2	0.48	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000536.3	0.48	ribosomal	nisin Q	Lactococcus lactis
BGC0001789.3	0.48	ribosomal	suicin 90-1330	Streptococcus suis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.53	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.50	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002346.3	0.49	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000506.4	0.49	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000542.3	0.49	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000556.5	0.49	ribosomal	streptin	Streptococcus pyogenes
BGC0000559.4	0.49	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002316.2	0.48	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000536.3	0.48	ribosomal	nisin Q	Lactococcus lactis
BGC0001789.3	0.48	ribosomal	suicin 90-1330	Streptococcus suis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for HMPREF0298_RS00435

HMPREF0298_RS11685

Leader:

MSDFTLDFAEGDAADTVSPQ

Core:

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

Core with dehydrations:

IDhbDhaKDhaLCysDhbPGCysIDhbGWMMCysNDhbVDhbKGCysDhaFDhbIGK

antiSMASH-DB 4.0 matches for HMPREF0298_RS11685

100.0%

NZ_GG667191.1and 1 other:

NZ_GG667191.1 Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

1

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

31

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

1

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

31

62.5%

NZ_AULX01000006.1and 1 other:

NZ_LT732548.1 Lanthipeptides: CZ379_RS19505_lanthipeptide

Lanthipeptides: H583_RS0100280_lanthipeptide

1

ITSKSLCTPGCITGWMMCNTVTKGCSFT

28

ITSKSLCTPGCITG +MC TKGC +

1

ITSKSLCTPGCITGVLMC--FTKGCEIS

26

60.0%

NZ_CP013655.1and 2 others:

NZ_CP013655.1 Lanthipeptides: ATZ35_RS03635_lanthipeptideNZ_CP013655.1 Lanthipeptides: ATZ35_RS03630_lanthipeptide

Lanthipeptides: ATZ35_RS03640_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSFTIGK

31

ITSKSLCTPGC+TG +M T+T CS IGK

1

ITSKSLCTPGCVTGVLMGCALKTITCNCSVGIGK

34

with 104 more sequence matches

Only the first 10 are displayed here.

60.0%

NZ_FNDP01000013.1:

Lanthipeptides: BLP54_RS11230_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIG

30

ITSKSLCTPGC+TG +M CN T T CS +G

3

ITSKSLCTPGCVTGVLMGCNNKTATCNCSVHVG

35

59.4%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSF

27

ITSKSLCTPGC TG +M T T GC F

1

ITSKSLCTPGCKTGALMTCPIKTATCGCHF

30

58.8%

NC_013656.1and 18 others:

NC_020450.1 Lanthipeptides: LILO_RS03015_lanthipeptideNZ_CP009472.1 Lanthipeptides: LG36_RS03420_lanthipeptideNZ_CP010050.1 Lanthipeptides: QI18_RS03300_lanthipeptideNZ_CP015903.1 Lanthipeptides: LLUC08_RS03255_lanthipeptideNZ_CP024954.1 Lanthipeptides: CV702_RS02990_lanthipeptideNZ_CP066300.1 Lanthipeptides: LacL0098_RS03205_lanthipeptideNZ_CP098790.1 Lanthipeptides: M5C73_RS03405_lanthipeptideNZ_JAALEQ010000006.1 Lanthipeptides: HFD15_RS10120_lanthipeptideNZ_JAJONJ010000005.1 Lanthipeptides: LSG16_RS07195_lanthipeptideNZ_JAQDWM010000007.1 Lanthipeptides: PGU01_RS08180_lanthipeptideNZ_JAQDXV010000018.1 Lanthipeptides: PGT80_RS11850_lanthipeptideNZ_JAQEIZ010000019.1 Lanthipeptides: PGX18_RS12025_lanthipeptideNZ_JAQEOU010000022.1 Lanthipeptides: PGV42_RS12330_lanthipeptideNZ_JAQEOV010000015.1 Lanthipeptides: PGW09_RS10765_lanthipeptideNZ_MTJP01000006.1 Lanthipeptides: BW151_RS03795_lanthipeptideNZ_MTJR01000005.1 Lanthipeptides: BW153_RS02980_lanthipeptideNZ_MTJS01000002.1 Lanthipeptides: BW154_RS09850_lanthipeptideNZ_RAGL01000022.1 Lanthipeptides: D6108_RS11395_lanthipeptide

Lanthipeptides: LLKF_RS13210_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T T CS + K

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

58.8%

NZ_CP065984.1and 2 others:

NZ_CP095737.1 Lanthipeptides: MXM90_RS03205_lanthipeptideNZ_JAQDXJ010000021.1 Lanthipeptides: PGU19_RS11740_lanthipeptide

Lanthipeptides: I6I21_RS02785_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T T CS + K

1

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK

34

58.1%

NC_015600.1and 14 others:

NZ_AP020310.1 Lanthipeptides: SAGCMC97051_RS07300_lanthipeptideNZ_ATVP01000001.1 Lanthipeptides: G574_RS11150_lanthipeptideNZ_BCFB01000042.1 Lanthipeptides: GOZ36_RS07980_lanthipeptideNZ_CP068708.1 Lanthipeptides: JM964_RS04040_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_FIKS01000005.1 Lanthipeptides: A0525_RS10015_lanthipeptideNZ_GL636070.1 Lanthipeptides: HMPREF9171_RS106200_lanthipeptideNZ_JADPLU010000018.1 Lanthipeptides: I4V82_RS09545_lanthipeptideNZ_JAKVIE010000017.1 Lanthipeptides: MK857_RS08340_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_LR594050.1 Lanthipeptides: FGL20_RS03485_lanthipeptideNZ_SSXN01000004.1 Lanthipeptides: FAJ36_RS04715_lanthipeptide

Lanthipeptides: SGPB_RS10680_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSF

27

+TSKSLCTPGC TG +M T T GC F

1

VTSKSLCTPGCKTGILMTCAIKTATCGCHF

30

57.6%

NZ_CP015405.2and 7 others:

NZ_CP015405.2 Lanthipeptides: A4V09_RS08295_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08290_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08285_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06240_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06235_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06230_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06225_lanthipeptide

Lanthipeptides: A4V09_RS08300_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSFT

28

ITSKSLCTPGC+TG +M T T GC T

1

ITSKSLCTPGCVTGILMTCPVQTATCGCQIT

31

57.6%

NZ_CP085954.1:

Lanthipeptides: LK411_RS13585_lanthipeptide

1

ITSKSLCTPGCITGWMMCNT----VTKGCSFT

28

ITSKSLCTPGC TG + C T T GC+ T

1

ITSKSLCTPGCPTGALGCFTQNCDPTNGCTIT

32

56.2%

NZ_JAMDIB010000004.1:

Lanthipeptides: M5P35_RS04030_lanthipeptide

1

ITSKSLCT-PGCITGWMM---CNTVTKGCSF

27

+TSKSLCT PGC TG +M T T GC F

1

VTSKSLCTPPGCKTGILMTCAIKTATCGCHF

31

55.9%

NZ_FBTD01000003.1:

Lanthipeptides: PB1E_RS03075_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T + GC + K

1

ITSVSLCTPGCKTGALMGCNMKTASCGCHVHVSK

34

54.8%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSF

27

+TS SLCTPGC TG +M T T GC F

1

VTSISLCTPGCKTGILMTCAIKTATCGCHF

30

MIBiG 4.0 matches for HMPREF0298_RS11685

58.8%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T T CS + K

1

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK

34

12.9%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

20

TVTKGCS

26

T+TK C

20

TLTKECQ

26

Lanthipeptide(s) for HMPREF0298_RS00440

HMPREF0298_RS11685

Leader:

MSDFTLDFAEGDAADTVSPQ

Core:

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

Core with dehydrations:

IDhbDhaKDhaLCysDhbPGCysIDhbGWMMCysNDhbVDhbKGCysDhaFDhbIGK

antiSMASH-DB 4.0 matches for HMPREF0298_RS11685

100.0%

NZ_GG667191.1and 1 other:

NZ_GG667191.1 Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

1

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

31

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

1

ITSKSLCTPGCITGWMMCNTVTKGCSFTIGK

31

62.5%

NZ_AULX01000006.1and 1 other:

NZ_LT732548.1 Lanthipeptides: CZ379_RS19505_lanthipeptide

Lanthipeptides: H583_RS0100280_lanthipeptide

1

ITSKSLCTPGCITGWMMCNTVTKGCSFT

28

ITSKSLCTPGCITG +MC TKGC +

1

ITSKSLCTPGCITGVLMC--FTKGCEIS

26

60.0%

NZ_CP013655.1and 2 others:

NZ_CP013655.1 Lanthipeptides: ATZ35_RS03635_lanthipeptideNZ_CP013655.1 Lanthipeptides: ATZ35_RS03630_lanthipeptide

Lanthipeptides: ATZ35_RS03640_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSFTIGK

31

ITSKSLCTPGC+TG +M T+T CS IGK

1

ITSKSLCTPGCVTGVLMGCALKTITCNCSVGIGK

34

with 104 more sequence matches

Only the first 10 are displayed here.

60.0%

NZ_FNDP01000013.1:

Lanthipeptides: BLP54_RS11230_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIG

30

ITSKSLCTPGC+TG +M CN T T CS +G

3

ITSKSLCTPGCVTGVLMGCNNKTATCNCSVHVG

35

59.4%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSF

27

ITSKSLCTPGC TG +M T T GC F

1

ITSKSLCTPGCKTGALMTCPIKTATCGCHF

30

58.8%

NC_013656.1and 18 others:

NC_020450.1 Lanthipeptides: LILO_RS03015_lanthipeptideNZ_CP009472.1 Lanthipeptides: LG36_RS03420_lanthipeptideNZ_CP010050.1 Lanthipeptides: QI18_RS03300_lanthipeptideNZ_CP015903.1 Lanthipeptides: LLUC08_RS03255_lanthipeptideNZ_CP024954.1 Lanthipeptides: CV702_RS02990_lanthipeptideNZ_CP066300.1 Lanthipeptides: LacL0098_RS03205_lanthipeptideNZ_CP098790.1 Lanthipeptides: M5C73_RS03405_lanthipeptideNZ_JAALEQ010000006.1 Lanthipeptides: HFD15_RS10120_lanthipeptideNZ_JAJONJ010000005.1 Lanthipeptides: LSG16_RS07195_lanthipeptideNZ_JAQDWM010000007.1 Lanthipeptides: PGU01_RS08180_lanthipeptideNZ_JAQDXV010000018.1 Lanthipeptides: PGT80_RS11850_lanthipeptideNZ_JAQEIZ010000019.1 Lanthipeptides: PGX18_RS12025_lanthipeptideNZ_JAQEOU010000022.1 Lanthipeptides: PGV42_RS12330_lanthipeptideNZ_JAQEOV010000015.1 Lanthipeptides: PGW09_RS10765_lanthipeptideNZ_MTJP01000006.1 Lanthipeptides: BW151_RS03795_lanthipeptideNZ_MTJR01000005.1 Lanthipeptides: BW153_RS02980_lanthipeptideNZ_MTJS01000002.1 Lanthipeptides: BW154_RS09850_lanthipeptideNZ_RAGL01000022.1 Lanthipeptides: D6108_RS11395_lanthipeptide

Lanthipeptides: LLKF_RS13210_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T T CS + K

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

58.8%

NZ_CP065984.1and 2 others:

NZ_CP095737.1 Lanthipeptides: MXM90_RS03205_lanthipeptideNZ_JAQDXJ010000021.1 Lanthipeptides: PGU19_RS11740_lanthipeptide

Lanthipeptides: I6I21_RS02785_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T T CS + K

1

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK

34

58.1%

NC_015600.1and 14 others:

NZ_AP020310.1 Lanthipeptides: SAGCMC97051_RS07300_lanthipeptideNZ_ATVP01000001.1 Lanthipeptides: G574_RS11150_lanthipeptideNZ_BCFB01000042.1 Lanthipeptides: GOZ36_RS07980_lanthipeptideNZ_CP068708.1 Lanthipeptides: JM964_RS04040_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_FIKS01000005.1 Lanthipeptides: A0525_RS10015_lanthipeptideNZ_GL636070.1 Lanthipeptides: HMPREF9171_RS106200_lanthipeptideNZ_JADPLU010000018.1 Lanthipeptides: I4V82_RS09545_lanthipeptideNZ_JAKVIE010000017.1 Lanthipeptides: MK857_RS08340_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_LR594050.1 Lanthipeptides: FGL20_RS03485_lanthipeptideNZ_SSXN01000004.1 Lanthipeptides: FAJ36_RS04715_lanthipeptide

Lanthipeptides: SGPB_RS10680_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSF

27

+TSKSLCTPGC TG +M T T GC F

1

VTSKSLCTPGCKTGILMTCAIKTATCGCHF

30

57.6%

NZ_CP015405.2and 7 others:

NZ_CP015405.2 Lanthipeptides: A4V09_RS08295_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08290_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08285_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06240_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06235_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06230_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06225_lanthipeptide

Lanthipeptides: A4V09_RS08300_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSFT

28

ITSKSLCTPGC+TG +M T T GC T

1

ITSKSLCTPGCVTGILMTCPVQTATCGCQIT

31

57.6%

NZ_CP085954.1:

Lanthipeptides: LK411_RS13585_lanthipeptide

1

ITSKSLCTPGCITGWMMCNT----VTKGCSFT

28

ITSKSLCTPGC TG + C T T GC+ T

1

ITSKSLCTPGCPTGALGCFTQNCDPTNGCTIT

32

56.2%

NZ_JAMDIB010000004.1:

Lanthipeptides: M5P35_RS04030_lanthipeptide

1

ITSKSLCT-PGCITGWMM---CNTVTKGCSF

27

+TSKSLCT PGC TG +M T T GC F

1

VTSKSLCTPPGCKTGILMTCAIKTATCGCHF

31

55.9%

NZ_FBTD01000003.1:

Lanthipeptides: PB1E_RS03075_lanthipeptide

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T + GC + K

1

ITSVSLCTPGCKTGALMGCNMKTASCGCHVHVSK

34

54.8%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

1

ITSKSLCTPGCITGWMM---CNTVTKGCSF

27

+TS SLCTPGC TG +M T T GC F

1

VTSISLCTPGCKTGILMTCAIKTATCGCHF

30

MIBiG 4.0 matches for HMPREF0298_RS11685

58.8%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

1

ITSKSLCTPGCITGWMM-CN--TVTKGCSFTIGK

31

ITS SLCTPGC TG +M CN T T CS + K

1

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK

34

12.9%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

20

TVTKGCS

26

T+TK C

20

TLTKECQ

26

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 21.85 out of 25.31

Confidence: strong

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 23.70 out of 29.25

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 21.40 out of 23.05

Confidence: strong

Additional weak hits (3):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.35 out of 26.33

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 19.74 out of 30.12

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 20.22 out of 30.12

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

HMPREF0298_RS11685 - Class I

Cleavage pHMM score: 4.80

RODEO score: 18

Monoisotopic mass: 3105.5 Da

Molecular weight: 3107.7 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3125.8 Da N = 1; 3143.8 Da N = 2; 3161.8 Da N = 3; 3179.8 Da N = 4; 3197.8 Da N = 5

HMPREF0298_RS11685 - Class I

Cleavage pHMM score: 4.80

RODEO score: 18

Monoisotopic mass: 3105.5 Da

Molecular weight: 3107.7 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3125.8 Da N = 1; 3143.8 Da N = 2; 3161.8 Da N = 3; 3179.8 Da N = 4; 3197.8 Da N = 5

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 133 - 214; Score: 37.2

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CatR	H2O2-responsive repressor
SypG	Regulator of biofilm formation and host colonization

NZ_GG667191.1 - Region 2 - terpene

Download region GenBank file

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Location: 372,533 - 393,549 nt. (total: 21,017 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

HMPREF0298_RS01895: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

Transferases: glycosyltransferase methyltransferase

HMPREF0298_RS01885: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

HMPREF0298_RS01890: methyltransferase

smcogs: SMCOG1089: methyltransferase

Isomerases: racemase

HMPREF0298_RS01860: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Ligases: synthetase

HMPREF0298_RS01900: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.53	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.49	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.43	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000913.3	0.43	other (other)	MK-8	Enterobacter cloacae
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002005.2	0.41	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0000906.3	0.40	other (other)	heme D1	Heliophilum fasciatum
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000905.5	0.40	other (other)	heme D1	Heliobacillus mobilis
BGC0001911.3	0.40	terpene	raimonol	Streptomyces anulatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.53	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.49	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.43	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000913.3	0.43	other (other)	MK-8	Enterobacter cloacae
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002005.2	0.41	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0000906.3	0.40	other (other)	heme D1	Heliophilum fasciatum
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000905.5	0.40	other (other)	heme D1	Heliobacillus mobilis
BGC0001911.3	0.40	terpene	raimonol	Streptomyces anulatus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

HMPREF0298_RS01900: PT_FPPS_like

Domain 1 (51 - 332): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (10):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.33 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.72 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.40 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.89 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.80 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.07 out of 27.78

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.12 out of 36.53

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.27 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.47 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.19 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description