LOCUS       NZ_GG667198            10375 bp    DNA     linear   CON 01-MAY-2024
DEFINITION  Corynebacterium lipophiloflavum DSM 44291 SCAFFOLD8, whole genome
            shotgun sequence.
ACCESSION   NZ_GG667198
VERSION     NZ_GG667198.1
KEYWORDS    WGS; RefSeq.
SOURCE      Corynebacterium lipophiloflavum DSM 44291
  ORGANISM  Corynebacterium lipophiloflavum DSM 44291
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Corynebacteriaceae; Corynebacterium.
REFERENCE   1  (bases 1 to 41277)
  AUTHORS   Qin,X., Bachman,B., Battles,P., Bell,A., Bess,C., Bickham,C.,
            Chaboub,L., Chen,D., Coyle,M., Deiros,D.R., Dinh,H., Forbes,L.,
            Fowler,G., Francisco,L., Fu,Q., Gubbala,S., Hale,W., Han,Y.,
            Hemphill,L., Highlander,S.K., Hirani,K., Hogues,M., Jackson,L.,
            Jakkamsetti,A., Javaid,M., Jiang,H., Korchina,V., Kovar,C., Lara,F.,
            Lee,S., Mata,R., Mathew,T., Moen,C., Morales,K., Munidasa,M.,
            Nazareth,L., Ngo,R., Nguyen,L., Okwuonu,G., Ongeri,F., Patil,S.,
            Petrosino,J., Pham,C., Pham,P., Pu,L.-L., Puazo,M., Raj,R., Reid,J.,
            Rouhana,J., Saada,N., Shang,Y., Simmons,D., Thornton,R., Warren,J.,
            Weissenberger,G., Zhang,J., Zhang,L., Zhou,C., Zhu,D., Muzny,D.,
            Worley,K. and Gibbs,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2009) Human Genome Sequencing Center, Baylor
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   2  (bases 1 to 41277)
  AUTHORS   Qin,X., Bachman,B., Battles,P., Bell,A., Bess,C., Bickham,C.,
            Chaboub,L., Chen,D., Coyle,M., Deiros,D.R., Dinh,H., Forbes,L.,
            Fowler,G., Francisco,L., Fu,Q., Gubbala,S., Hale,W., Han,Y.,
            Hemphill,L., Highlander,S.K., Hirani,K., Hogues,M., Jackson,L.,
            Jakkamsetti,A., Javaid,M., Jiang,H., Korchina,V., Kovar,C., Lara,F.,
            Lee,S., Mata,R., Mathew,T., Moen,C., Morales,K., Munidasa,M.,
            Nazareth,L., Ngo,R., Nguyen,L., Okwuonu,G., Ongeri,F., Patil,S.,
            Petrosino,J., Pham,C., Pham,P., Pu,L.-L., Puazo,M., Raj,R., Reid,J.,
            Rouhana,J., Saada,N., Shang,Y., Simmons,D., Thornton,R., Warren,J.,
            Weissenberger,G., Zhang,J., Zhang,L., Zhou,C., Zhu,D., Muzny,D.,
            Worley,K. and Gibbs,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2009) Human Genome Sequencing Center, Baylor
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000159635.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 01:00:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,353
            CDSs (total)                      :: 2,297
            Genes (coding)                    :: 2,213
            CDSs (with protein)               :: 2,213
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 21 of 84
            Pseudo Genes (incomplete)         :: 64 of 84
            Pseudo Genes (internal stop)      :: 11 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:27:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7834
            Orig. end                         :: 18209
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GG667198.1.
            This sequence is generated as a part of the Human Microbiome
            Project (HMP), funded by the National Institutes of Health (NIH),
            National Human Genome Research Institute (NHGRI) Large-Scale
            Sequencing Research Network and the National Institute of Allergy
            and Infectious Diseases (NIAID) Microbial Genome Sequencing Centers
            Program.  The mission of the HMP is to generate resources to enable
            the characterization of the human microbiota, the microbial
            communities living within human bodies, and analyze the role of
            these organisms in human health and disease.  See
            http://www.hmpdacc.org/ for more information.
            The sequenced strain was obtained from DSMZ.  The genomic DNA was
            prepared from a single bacterial isolate.  The sequence generated
            included at least 10 fold coverage of Roche/454 Lifesciences FLX
            fragment data and at least 8 fold clone coverage of Roche/454
            Lifesciences paired-end data.
            The Roche/454 Lifesciences sequence was assembled using the
            Roche/454 Lifescience Newbler assembler.  The contigs from the
            Newbler assembly were aligned with mapping tools such as Mosaic and
            Crossmatch and these data were used for error correction for this
            version of the draft assembly.  This draft assembly meets the HMP
            draft quality standards (more than 90% of the genome is included in
            contigs, more than 90% of a core set of bacterial genes are found
            with > 30% identity and > 30% length; more than 90% of the bases in
            the assembly have more than 5 fold sequence coverage, the contig
            N50 length is greater than 5kb, the scaffold N50 length is greater
            than 20kb, and there is less than 1 gap per 5kb).
            This is a reference genome for the Human Microbiome Project.
            This project is co-owned with the Human Microbiome Project DACC.
            Genome Coverage: 32x
            Sequencing Technology: 454; ABI
            Annotation was added to the contigs in April 2009.
            Product names were updated in June 2013.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5375](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5375
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    637..650
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.92"
                     /tool="antismash"
     gene            998..2803
                     /locus_tag="HMPREF0298_RS10535"
                     /old_locus_tag="HMPREF0298_1671"
     CDS             998..2803
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003859411.1"
                     /locus_tag="HMPREF0298_RS10535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1671"
                     /product="BCCT family transporter"
                     /protein_id="WP_006841174.1"
                     /transl_table=11
                     /translation="MSTNTSIKKRGLQSDPLIFYTSVGFIALFVGLTVIFGDRAREAYS
                     SISTALMNNFSWFYIGGVSMVLVFLIVVFVSKYGNLRLGDDEDEPEYSLPVWFAMLFAA
                     GMGATLLFWGAAEPLHHAYNPPRGDYEPMSREAITQAFEFTYYHFGIHMWVIFTLPGLA
                     LGYFSYKRKMPARMSSLFSPLLGKRVYEWPGKLIDAAGIIGTVFGIGVSVGLGVLQISA
                     GMNIMWSVPLITPVQLGIILAITAAACLSVASGLDKGIKILSNLNIAATVVLMLFVLLA
                     GPTLRLIGQITESFGIYANSLPELMFWVDSYNDNPGWHATWTAFYWAWTICWAPFVGMF
                     FARISKGRTVRAFIGGTLLLPTTFDMIWFSIFGRAATEIETANPGVLTGPVVEQGDTPQ
                     ALFTLLSQYPLYGIVGSLALVVIILYFVTSMDSAAMVMDMFASGEENKTPTYYKVGWVI
                     AIGVVTAALLFINDTGIQALQEVVIIIALPFFILYFFIMFALLKAMNDDSAARRRVRTR
                     QWEKTDSAEKLEEAENRPAPGYDAEGNELARPELEYDPEEGGWRLSDSLIIEGDLAIGG
                     EVDQEWSEESEPTVKIVDEDSPKRH"
     gene            complement(2800..3579)
                     /locus_tag="HMPREF0298_RS10540"
                     /old_locus_tag="HMPREF0298_1672"
     CDS             complement(2800..3579)
                     /EC_number="2.8.1.10"
                     /GO_function="GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767935.1"
                     /locus_tag="HMPREF0298_RS10540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1672"
                     /product="thiazole synthase"
                     /protein_id="WP_006841175.1"
                     /transl_table=11
                     /translation="MLTIAGREFSSRLIMGTGGASSMDMLERCLIASRTELTTVAMRRH
                     AAGGSGESVFDMLRRLGIAPLPNTAGCRTARDAVLTAELAREALHTNWVKLEVIADDRT
                     LLPDVVETVDACEMLINAGFTVLAYTSDDPVAAKRLEDIGAAAVMPLGSPIGTGLGILN
                     PHNIELICSRAEVPVLIDAGVGTASDAVLAMELGCSGVLLASAINRSQDPEAMAHAMRL
                     AVEAGALAAGAGRIPKREHAVASSSFEGLASWADQVL"
     gene            complement(3585..3794)
                     /gene="thiS"
                     /locus_tag="HMPREF0298_RS10545"
                     /old_locus_tag="HMPREF0298_1673"
     CDS             complement(3585..3794)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883098.1"
                     /locus_tag="HMPREF0298_RS10545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1673"
                     /product="sulfur carrier protein ThiS"
                     /protein_id="WP_006841176.1"
                     /transl_table=11
                     /translation="MSTHILVNNTARDTDAATVSELVHEVLGYVPDAGTAVAIDSAVVP
                     RSKWESTHLADGARVDILTAVQGG"
     gene            complement(3791..4885)
                     /gene="thiO"
                     /locus_tag="HMPREF0298_RS10550"
                     /old_locus_tag="HMPREF0298_1674"
     CDS             complement(3791..4885)
                     /EC_number="1.4.3.19"
                     /GO_function="GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="thiO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005291547.1"
                     /locus_tag="HMPREF0298_RS10550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1674"
                     /product="glycine oxidase ThiO"
                     /protein_id="WP_006841177.1"
                     /sec_met_domain="DAO (E-value: 4.2e-56, bitscore: 182.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGTPTAVVGAGIIGLSTALTLADRGHSVTVYDPAPARGASHYAG
                     GMLAPASEVVYKQDPLLPLMQASASRYPELIALCRKYSDLPTGYRTEGTLVVARDRADS
                     TFLDDLLSYQHARGVHAQRLTVRQARALEPALSPALSSAVLSSSDHQVQPRLFTRALVD
                     ACTNAGVSFIRELVTDVSHLPQEQVVIAAGHGAKDISGWGEGDKNPLQLRPVYGDILHL
                     RAPAHLHPLATRVVRGVVEGRAIYVIPREDATLTVGATSREDGRASAQAQGVYQLLRDA
                     IEILPGIEDCDFVEAGAGARPATPDDLPYLGRVSERVVVSTGYFRHGILLAALAARCAA
                     ELVEKQKPCLDLSACDPMRHRRTQ"
     misc_feature    4993..5007
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            5001..5375
                     /locus_tag="HMPREF0298_RS10555"
                     /old_locus_tag="HMPREF0298_1675"
     CDS             5001..5375
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841178.1"
                     /locus_tag="HMPREF0298_RS10555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1675"
                     /product="ectoine synthase"
                     /protein_id="WP_006841178.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-34, bitscore:
                     110.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFTRSRNDVEKVEWGGGTSERVLTAKDNMGFAVAHTVVRAGTESK
                     LQYRNHLEACYCIGGSGEVEDTEGNIYPITPGTIYVLDEHDAHFLRGGKDEDLILVSIF
                     NPAITGDEKHTLSEDGFSSY"
     misc_feature    complement(5458..5473)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.39"
                     /tool="antismash"
     gene            5499..6734
                     /locus_tag="HMPREF0298_RS10560"
                     /old_locus_tag="HMPREF0298_1676"
     CDS             5499..6734
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005291533.1"
                     /locus_tag="HMPREF0298_RS10560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1676"
                     /product="Nramp family divalent metal transporter"
                     /protein_id="WP_040423176.1"
                     /transl_table=11
                     /translation="MSTVETTTAPQRKSGMFGPGLLIAASFIGPGTVTTATVTGASYGY
                     ALVWAIVFSIVATIILQEMSVRLGLAARLSTGEALRQTFGSQIIKWLMIILVVSAIGIG
                     GAAYAGGDTTGTALALASVTSLPHVLLSAGVAAVVLALLLSGSYAFLEKVMTVLVGILA
                     LTFVITAISVGPDLGAMLRSTFVPRVPDGAVLSAVALIGTTVVPYNVFLHSNLVQEKWG
                     DEPRDPALKKARIDNVVSISLGGVITLAIVATAAATMFAQGLQAESAADLAEPLRPMLG
                     DAAPWLLAIGLFAAGMTSAVAGPMGAAYAICGVMGWPTDMKSQRFRAIVVVVVLCGAAI
                     AISGFNPIQVIILAQAANGILLPVIAFFLLITMNNKRLLGEHANSLVGNIVGGLIFLIT
                     LVLGGLSLADLF"
     gene            complement(6738..7178)
                     /locus_tag="HMPREF0298_RS10565"
                     /old_locus_tag="HMPREF0298_1677"
     CDS             complement(6738..7178)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018581045.1"
                     /locus_tag="HMPREF0298_RS10565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1677"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_006841180.1"
                     /transl_table=11
                     /translation="MIAISPLAQLTALQTHALFKLRVDVFVVEQQCPFAEIDDIDAHPD
                     TRHLLAWSDEDPAELLGCARVFPTDSGSRFGRFVVAPAARGTGLGHEIVRTGIAYTQRF
                     SGDLVIEAQSGLVGYYTGFGFVAEGEEFLDAGIPHVMMRLRR"
     gene            complement(7189..8358)
                     /locus_tag="HMPREF0298_RS10570"
                     /old_locus_tag="HMPREF0298_1678"
     CDS             complement(7189..8358)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012360353.1"
                     /locus_tag="HMPREF0298_RS10570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1678"
                     /product="MFS transporter"
                     /protein_id="WP_006841181.1"
                     /transl_table=11
                     /translation="MSSRKVFYSVILFLVTAGWAANHFAAVLVALKERAHLEPLLVNGA
                     YGIYAAGLFPCLLAGGILADRFGGRPIVIAGTIVSALGNLGLAFSHSAAALLSGRFVVG
                     LGVGLVVSAGTAWAARLRGAQGATLAGIFLTSGFALGPIFSGIFAYAQPRIWELYAVTI
                     ALSLLAIVVSLVIGDTPHHAVTQGPDGPAPAPAAAPAQRSSTKALATALPVAVWVFASI
                     TTAVVGLAARVAHYFPTGVFMPGIAAGLGFGTALVLQALGRRFDWGPRAGVIGALCSAT
                     GLAIAGFAGTNPTLAAFAAATIALGAAYGLCLRDGLLDVDTYAPLHARGRVLGIYYVGT
                     YIGFALPPLLQWLEPLAGPTVPLLVLAALALTSAVVRFTQIKTGYLSRT"
     gene            complement(8364..9023)
                     /locus_tag="HMPREF0298_RS10575"
                     /old_locus_tag="HMPREF0298_1679"
     CDS             complement(8364..9023)
                     /EC_number="2.5.1.3"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1280: thiamine-phosphate pyrophosphorylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004600027.1"
                     /locus_tag="HMPREF0298_RS10575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1679"
                     /product="thiamine phosphate synthase"
                     /protein_id="WP_006841182.1"
                     /transl_table=11
                     /translation="MLSCQLDLRCYFVTGSGSERDIVTLARDAARAGAGVIQVRSKPIS
                     ARDLYSLGREVAHAVREANPATRVLIDDRVDVALALRDEGVHGVHLGQDDLDVRVARRL
                     LGPEAIIGLTTGTLQLVRAANELAGDIDYVGAGPFRATPTKDSGRPLLGVDGYRPLVAE
                     SLVPVVAIGDVTVDDAAELASTGVDGLALVRGIMNAEDTGAYVARVLSEFERGAVD"
     gene            complement(9016..10245)
                     /locus_tag="HMPREF0298_RS10580"
                     /old_locus_tag="HMPREF0298_1680"
     CDS             complement(9016..10245)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012714908.1"
                     /locus_tag="HMPREF0298_RS10580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF0298_1680"
                     /product="DUF418 domain-containing protein"
                     /protein_id="WP_006841183.1"
                     /transl_table=11
                     /translation="MDKTPAKQRLLLPDIVRGVSLLGIAAANSVQAWITTGSSGAGAPG
                     DTLGGVDPSSGVDAFFAVFSAMFVHVRGLPMFSTLLGFGFGLVVTSLYRKHYPLRDARR
                     VLLRRYGTLAALGLIHMLAIFYGDIMLTYGLIGVLMALLFTVSAKWLRLIAYILLGFFA
                     VTGTAGAFAVYFFEPGALPDMRAPTTELLTVGDYFSANFSAAMLMLTSIPFAVGSLAGL
                     SIIGYVWATEGYLVNVDKHRAILRRWVYVAVAIILLLGLPWGLAAIGVIDPGLEEFFWI
                     LNQSWGPFTGPGILAAFALATNGMQKRAAETQSAPRWAYPLIALGKRSMSGYLAQSLLF
                     IVLASPFGFGLGLDANVTGKLGVGLLVWLITLLLASILEATRTPGPFEWAHRRLAYGRT
                     GAIEPRPAHA"
ORIGIN
        1 tccgggtcgg cttcccacac gctggtgggc aggtggtggg ttgcgcccgg gatgtcggag
       61 atcgcctttt cgtggtcttc ccgcacgtcc agcccgaggg cctcgccgcg agcgagcctg
      121 tctagcagcg cgcgtgccga cgccccctcc acctcgcagg cgccggcacc tgccgcgtcg
      181 taggttccga aacccgtcac aaggtctcgc gccggatcgc gggcgacgtc gaaggtgcgg
      241 attgttgcac tcatcgcgtc gtacatccgt agcctaccca ccactgactc gccgacaccg
      301 gtgatgaatt tgatcacttc ggtcgccatc agcccaccga caacgctggt ggtcaccccg
      361 agtactcccg cggtggcgca gtcgggcacc gagtccgcgc cgggttgggt ggggtagagg
      421 tcgcgcagac ccgccccatc ggccggcgcg ccggggccgc tgaaccacaa ggcgacgtcg
      481 ccgcggtagc gcagcaccga gccccagacg aggggtacgc cggcaatttc ggcggcgtcg
      541 gcggcgagga acttggtggc aaacgtgtca gagccgtcga caagcacgtc gacacccgcg
      601 agcatatcca gcgcgttgga tgcagtgaac cggccgttga tgcagttcag ctcgatgccg
      661 ggttgcagtg cgcgaagccg ctcgcccgcg acgtccacct tcgcgcggcc cacgtcgtcc
      721 gcgccgaaga gaatctggcg gtggatgttg cttacgtcca cggtgtcgtc gtcaatcacg
      781 gtgattcgcc cgattccggt gccggccagg gtctgcatca gcgggcagcc gagcccgccg
      841 gcgccgatga caaagacgtg ggcgttgtgc agcgccgtct gctgctcgag gccgaacccg
      901 ggcaggttca tctggcgggc ggtgcggcga agttcgtggt ggggcaggtc aggcatatct
      961 tccaccctat tgaagtgagg cgctacactg cgcacttatg tcgacgaaca catccattaa
     1021 aaagcgtggg ttgcagtccg acccgctgat tttctacacc tccgtgggct tcatcgccct
     1081 gttcgtcggg ctcaccgtca tcttcggcga ccgcgcccgc gaagcgtact cgtcgatttc
     1141 cacggcgctg atgaacaatt tctcctggtt ctacatcggc ggcgtgtcca tggtcctggt
     1201 gttcctgatc gtggtgttcg tgtccaagta cggcaacctg cgcctcggtg atgacgagga
     1261 cgagcccgag tactccttgc ccgtttggtt cgcgatgctg ttcgccgcgg gcatgggtgc
     1321 cacgctcctg ttctggggcg ccgcggaacc gctgcaccac gcctacaacc cgccgcgcgg
     1381 tgattacgag ccaatgagcc gcgaggcgat cacccaagcg ttcgagttca cctactacca
     1441 cttcggcatc cacatgtggg tgatcttcac cctcccgggg ctggcgctag gttacttcag
     1501 ctacaagcgc aaaatgccgg cgcgcatgtc ctcgctgttc tccccgcttc taggcaagcg
     1561 cgtctacgag tggccgggca agcttatcga cgccgccggc atcatcggca ccgtcttcgg
     1621 catcggcgtc tccgtcggct tgggtgtgct gcagatttcc gccgggatga acatcatgtg
     1681 gtcggttccg ctgatcaccc cggtgcagct cggcatcatc ctcgcgatca cggccgccgc
     1741 gtgcctctcc gttgcctccg gtctggataa gggaatcaag atcctgtcca acctcaatat
     1801 cgcggcgacc gtcgtactga tgctgttcgt gctgctggcg ggcccgacgc tcaggctcat
     1861 cgggcagatc accgagtcct tcggcatcta cgccaactcg cttccggagc taatgttctg
     1921 ggtcgattcc tacaacgaca acccgggctg gcacgcgacg tggaccgcgt tctactgggc
     1981 gtggacgatt tgctgggcgc cgttcgtggg catgttcttc gcccgcatct ctaagggccg
     2041 cacggtgcgc gcgttcatcg gcggcaccct gcttctgccg acgaccttcg acatgatctg
     2101 gttctccatc ttcggccgcg ccgcgaccga gatcgagacg gccaacccgg gcgtgctcac
     2161 cggccccgtg gtggagcagg gcgacacccc gcaggcgctg ttcaccctcc tgtcgcagta
     2221 cccgctgtac ggcatcgtcg gctcgctggc cctggttgtc atcatcttgt acttcgtcac
     2281 ctcgatggat tccgccgcga tggtgatgga tatgttcgcc tcgggtgagg agaacaagac
     2341 cccgacgtac tacaaggtcg gctgggtcat cgccattggc gtggtcacgg cggccctgct
     2401 gtttatcaac gacaccggca tccaggcgct gcaggaagtc gtgatcatta tcgctctgcc
     2461 gttcttcatc ctctacttct tcatcatgtt cgcgctactc aaggcgatga acgacgactc
     2521 cgccgcgcgc cgccgcgtcc gcacccgcca gtgggagaag accgacagtg ccgagaagct
     2581 ggaggaggcg gaaaaccgcc ccgcgccggg ttacgacgcc gagggcaacg agctggcccg
     2641 ccccgagctg gagtacgacc cggaggaggg cggttggagg ctgtcggact cgctgatcat
     2701 cgagggtgac ctcgcgatcg gcggcgaggt cgaccaagag tggagcgagg aatcggaacc
     2761 cacggtgaaa atcgtcgacg aggatagccc caagcgccac tagagcacct ggtcggccca
     2821 gctcgccagg ccttcaaagc ttgacgacgc caccgcgtgt tcccgcttcg gaatccgccc
     2881 ggcgcccgcc gccagcgccc cggcttcaac agcgaggcgc atcgcgtggg ccatcgcctc
     2941 cgggtcctgg ctgcggttaa tggcgctggc caacaaaacc ccggagcagc cgagttccat
     3001 ggcgagcacg gcgtcggagg cggtgccgac gccagcgtcg ataagcacgg gcacttccgc
     3061 gcgcgaacag atcagctcga tgttgtgcgg gttgaggatg cccaacccgg tgccgatcgg
     3121 cgaacccagc ggcatcaccg ccgccgcgcc gatgtcctca aggcgcttcg ccgccacagg
     3181 gtcgtccgag gtgtaggcga gcaccgtaaa gccggcgttg atgagcatct cgcaggcgtc
     3241 gacggtctcg accacgtcgg gcagtagcgt gcggtcgtcg gcgatgacct cgagcttgac
     3301 ccagttggtg tgcagcgctt cgcgggcgag ctcggcggtg agcaccgcgt cgcgcgcggt
     3361 tctgcagccc gccgtgttag gcagtggcgc gataccgagc cgccgcagca tgtcgaagac
     3421 tgactcgccg ctgccaccgg cggcgtggcg gcgcatcgca acggtcgtca gctccgtgcg
     3481 ggaggcaatc aggcagcgct cgagcatgtc catcgagctc gccccgccgg tgcccatgat
     3541 gaggcgggag gaaaactccc ggccagcgat agtaagcacc tgtcctagcc cccctggacg
     3601 gcggtgagga tgtccacgcg cgcaccgtcg gcaagatgcg tcgactccca cttcgaacgc
     3661 gggacgacgg ccgagtcgat cgcgacggcc gtgccggcgt cgggcacgta cccgagcacc
     3721 tcgtgcacga gctcgctgac cgtcgccgca tcggtgtccc gcgcggtgtt gttgaccaaa
     3781 atgtgtgtgc tcattgtgtc ctcctgtggc gcatcgggtc gcacgcactc aagtctaggc
     3841 agggcttttg cttctcgacg agctccgctg cacaccgcgc cgccagcgcc gccagcaaaa
     3901 tcccgtggcg gaagtacccg gtgctgacaa ccacgcgctc gctgacgcgc ccgaggtagg
     3961 gcagatcgtc cggcgtggcg ggccgggcgc ccgcgccggc ctcgacgaaa tcgcagtcct
     4021 cgatgccggg aaggatctca atcgcgtcgc gcagaagctg gtacacgccc tgggcctgcg
     4081 cgctcgcgcg gccatcctcg cggctggtcg cgccgaccgt gagcgtcgcg tcctcgcgcg
     4141 ggatgacgta gatcgccctg ccctccacca caccgcggac caccctggtg gcgaggggat
     4201 gcaggtgcgc cggggcccgc aggtgcagga tgtcgccgta gaccgggcgc agctgcagcg
     4261 ggtttttgtc cccctcgccc cacccggaga tgtctttcgc gccgtggccg gcggcgatga
     4321 cgacttgctc ctgcggcagg tgggagacgt cggtgacgag ctcgcggatg aagctcaccc
     4381 cggcgttggt gcaggcgtcg acaagcgcgc gcgtgaacag ccgcggctgg acctgatggt
     4441 cgctgctgct gagcaccgcc gaggacagcg cgggagacag tgcgggttcc agcgcccggg
     4501 cctggcgcac cgtgaggcgc tgcgcgtgca ctccgcgggc gtgctggtag ctgagcagat
     4561 cgtccagaaa tgtgctgtcg gcgcggtcgc gagcaaccac gagggtgccc tcggtgcggt
     4621 agcccgtcgg caggtcggag tacttccggc acaacgcgat cagctccggg taacggctgg
     4681 ctgaggcctg catcagcggt agaagcgggt cttgcttgta caccacctct gaagccggcg
     4741 cgagcattcc gcccgcgtag tgggaggcgc cgcgggctgg tgcagggtcg tagacggtga
     4801 ccgaatggcc gcgatctgcc agggttaggg cggtgctcaa cccgatgatt cccgcgccga
     4861 cgacggcggt tggcgtgcca gacatggtcc cattgttcca cacgcgaagg tatctcaggc
     4921 tcgcgcccga gcgcgcggag tttacgtagg gttgaaaggt gtaacgcaat cgccaacacg
     4981 taaatcttga ggaggatttc atgttcacca ggtctcgcaa cgatgtggaa aaggttgagt
     5041 ggggcggagg gacctccgag cgcgtgctca ccgcgaagga caacatgggc ttcgcggtgg
     5101 cgcacaccgt ggtgcgcgcg ggcaccgagt ccaagctgca gtaccgcaac cacctggagg
     5161 cgtgctattg catcggcggc tccggtgagg ttgaagacac cgagggcaac atctacccga
     5221 tcacgccggg caccatctac gtactcgacg agcacgacgc ccacttcctg cgcggcggca
     5281 aggacgagga tctgatccta gtcagcatct tcaacccggc gatcaccggc gacgagaagc
     5341 acaccttaag cgaggacggg ttcagctcct actaaaggca tctctgtgag gcgctaggga
     5401 agacaggtag tgaaatgcac cccgcggggt agcgcgacag tactaagctg tcaccaacgt
     5461 gatgtaactc acattcatag acggattcga ggtacgcgat gtccactgtc gaaaccacca
     5521 cagcgccaca gcgtaagtcc ggcatgtttg ggccggggct gctcatcgcg gcgtcgttca
     5581 tcggcccggg tacggtcacc acagccacgg tgaccggcgc gagctacggc tacgcgctcg
     5641 tctgggcgat tgttttctcc atcgtggcaa cgatcatcct gcaggaaatg tcggtgcgcc
     5701 tcggactcgc ggctcgcctc agcaccggtg aagcgctgcg tcagacgttt ggttcgcaga
     5761 ttatcaagtg gctgatgatc atcttggtcg tctccgcaat cggcatcggc ggcgctgcgt
     5821 acgccggggg cgacacgacg ggcaccgcgc tcgcgctggc cagcgtcaca agcctgccgc
     5881 acgtgttgct ctccgccggg gtggccgcgg tggtgctggc cttgctactt tcaggaagtt
     5941 acgcgtttct agaaaaggtg atgacggtgc tggtgggcat cctggcgctg accttcgtga
     6001 tcacggccat cagcgtgggg cccgacctcg gggccatgct gcgctccacc ttcgtgccca
     6061 gggtgccgga cggcgctgtt ctctccgcgg tcgcgctcat cggtacgacg gtcgttccct
     6121 acaacgtctt cttgcactcc aatctggtgc aggaaaagtg gggagacgag ccgcgggacc
     6181 ccgcactgaa gaaggcgcgg atagacaacg ttgtttccat cagcctcggt ggtgtgatca
     6241 ctttggcgat cgtcgcaacg gccgctgcga cgatgtttgc gcaaggactt caggcggaat
     6301 ccgccgccga cctcgccgag ccgcttcgcc cgatgcttgg cgacgccgcc ccgtggctgc
     6361 tcgcaatcgg cctgttcgcc gcgggcatga cctccgctgt cgccggtcca atgggcgcgg
     6421 cgtacgcgat ctgcggcgtc atgggctggc ccacggacat gaagagccag cgcttccgcg
     6481 ccatcgtggt ggtcgtggtc ctgtgcggcg cggcaatcgc gatctccggg ttcaacccga
     6541 tccaggtcat catcctcgcc caggccgcga acggcatctt gttgcccgtc atcgcgtttt
     6601 tccttctgat cacgatgaac aacaagcgtc tgctgggcga gcacgcgaat tctctggtgg
     6661 gaaacatcgt cggcgggctg atcttcctga tcacactcgt cctcggcggt ctatcgctcg
     6721 ccgacctgtt ttagctttca gcgccgcagc cgcatcatca cgtgcgggat gccggcatcg
     6781 aggaattcct cgccctcggc gacgaagccg aagccggtgt agtaacccac cagcccggat
     6841 tgggcctcga taacaaggtc gccggagaac cgctgggtgt aggcgatgcc ggtgcgcacg
     6901 atctcatggc cgaggcccgt gccacgcgca gctggggcaa cgacgaagcg gccgaaccgg
     6961 ctgccggagt cggtgggaaa cacccgtgcg cacccgagca gctcggccgg atcctcatcc
     7021 gaccacgcaa gaaggtggcg ggtgtccggg tgcgcgtcaa tgtcgtcgat ctcagcaaac
     7081 gggcactgct gttcgacgac gaacacgtca acgcgcagct tgaacagggc atgggtctgc
     7141 agagcggtaa gctgcgccag cggcgagatc gcaatcatgg ccccaagctt aggtgcggga
     7201 gaggtagccg gtcttaatct gggtaaagcg cacgacggct gaggtcaggg ccaaggcggc
     7261 gagcacgagc aggggcaccg tcggcccggc cagcggctct agccactgta acagcggcgg
     7321 cagggcgaat ccgatgtaag tgcccacgta gtagatgccg aggacgcggc cgcgcgcgtg
     7381 gaggggagcg tatgtgtcga cgtcgagaag cccatcgcgc aggcacaaac cgtacgccgc
     7441 acccaacgcg atggtcgctg cggcgaaggc tgcgagcgtg gggttggttc cagcgaagcc
     7501 ggcaatcgcc agtcccgtcg cggagcacag cgcgccgatg acgccggcgc gcgggcccca
     7561 gtcgaagcgc ctgcccagag cctggagcac aagcgcggtg ccgaagccga gaccggcggc
     7621 gattcccggc atgaacaccc cggtagggaa gtagtgggcc acgcgagcgg cgagcccgac
     7681 gaccgcggta gtgatggagg cgaagaccca caccgccacc ggcaatgccg tcgccagcgc
     7741 cttggtggat gagcgctgcg ccggtgccgc ggcgggcgct ggtgcggggc cgtcggggcc
     7801 ctgggttacc gcgtggtggg gtgtgtcgcc gatgaccagg gagaccacga tggccagcag
     7861 cgacagcgcg atggtcaccg cgtagagctc ccagatccgc ggctgggcat aggcgaaaat
     7921 tccggagaag atcggcccga gggcgaagcc ggaggtcaaa aagatgccgg cgagggtcgc
     7981 accttgcgcg ccgcggagcc tcgccgccca cgccgttccc gcgctgacga caagtccgac
     8041 accgagcccg acgacgaagc ggcccgacag cagggcggcg gcgctgtgcg agaaggcaag
     8101 gcctaggttt ccgagcgcgg agacgatcgt gcccgcaatg acaatcgggc ggcctccgaa
     8161 gcggtcggcg aggatcccgc cggccagaag gcacggaaac agaccggctg cgtagattcc
     8221 gtatgcgccg ttgaccagca gcggctcgag gtgggcgcgc tctttaaggg ccacgagaac
     8281 ggccgcgaag tggttcgcgg cccatcctgc cgtgacgagg aagaggatga cgctgtagaa
     8341 aactttgcgg ctggacatgg ggactaatct actgcgccgc gttcgaactc ggagagtacg
     8401 cgcgccacgt aggcgccggt gtcctcggcg ttcatgatgc cgcgcaccag agcgagcccg
     8461 tcgacgccgg tggaggcgag ctcggcggcg tcgtcgaccg tgacatcgcc gatggctacg
     8521 acgggcacga gggactcggc gacgaggggg cggtagccgt cgacaccgag tagcggccgg
     8581 cccgagtcct tcgtcggggt ggcgcgaaac ggcccggccc cgacgtagtc gatgtctcca
     8641 gctagttcat tggccgcgcg cacgagctga agcgtccccg tggtcagtcc gatgatcgct
     8701 tccgggccga gtagccgccg ggcaacgcgg acatcgaggt cgtcttggcc gaggtggacg
     8761 ccgtggacac cctcgtcgcg caaggcgagc gcgacgtcga cgcggtcgtc gataagcacg
     8821 cgcgttgcgg ggttggcctc gcgcaccgcg tgggcaacct cgcggccgag gctgtagagg
     8881 tcgcgcgcgg agatgggttt gctgcgcacc tggatgacac cggcgccggc gcgcgcggcg
     8941 tctcgcgcga gggtgacgat gtcgcgctcg ctaccgctcc cggtgacaaa gtaacagcgc
     9001 aggtcgagct ggcaactaag catgtgcggg cctcggttcg atcgcgccgg tgcgcccgta
     9061 ggcgaggcgg cggtgcgccc actcgaatgg gccgggggtg cgcgtggcct cgaggatgga
     9121 ggcgagaagc agcgtgatca gccagacgag caggcccacc ccgagcttgc cggtgacgtt
     9181 cgcgtccagg ccgagcccga acccgaacgg cgaagcgagg acgatgaaca gcagcgattg
     9241 cgcgaggtag cccgacatgg agcgcttgcc tagcgcaatg agggggtagg cccagcgggg
     9301 cgccgactgc gtctcggctg cgcgcttttg catcccgttc gtcgccagcg cgaacgcggc
     9361 gaggataccg gggccggtga atggtcccca ggattggttg aggatccaga aaaactcctc
     9421 gaggcccggg tcgatcaccc cgatcgccgc gagaccccac ggaagcccca gcagcaggat
     9481 gatcgccacg gcgacataca cccatcggcg cagaatagcg cgatgcttat cgacgttcac
     9541 caggtagccc tccgtcgccc agacgtagcc gatgatgctc agcccggcga gagaaccgac
     9601 cgcgaagggg atcgaagtga gcatcagcat cgccgcggaa aagttggcgc tgaagtagtc
     9661 acccacggtt aacagctccg tggtgggggc gcgcatatcg ggcagggccc cgggctcgaa
     9721 gaagtacaca gcaaaagcgc cggcggtgcc ggtaacggcg aaaaatccca gcaagatgta
     9781 ggcgatcagg cgcagccact tcgcgctgac ggtgaacagt agagccatga gcacgccgat
     9841 gagcccatag gtgagcatga tgtcgccgta gaagatcgcg agcatgtgga ttagccccag
     9901 cgcagcgagg gtgccgtagc ggcgcagaag cacccggcgc gcgtcgcgca gcgggtagtg
     9961 cttgcggtac aggctggtca ccactagccc gaagccgaag ccgagcagcg tggaaaacat
    10021 cggcaggccg cgtacgtgga cgaacatcgc ggaaaacacg gcgaagaagg catccacccc
    10081 gctcgaaggg tcgacgccgc cgagggtgtc gcccggcgcg ccggcaccgg atgagccagt
    10141 ggtgatccag gcttggaccg agttggctgc ggcgatgccc agcagggaga cgccgcggac
    10201 gatgtcgggg aggagcagtc tttgcttcgc gggtgttttg tccatgccca caacctactc
    10261 gggtggctta ctcggggagg tagacctgcg agccgtgttc gcggaaaaca tgcgccatgt
    10321 gctgctcgcc gaggacggcg tcgacggtgg gcatgcccag ggattcgatg gtgtc
//
