LOCUS       NZ_GG657757            10399 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Streptomyces viridochromogenes DSM 40736 supercont1.1, whole genome
            shotgun sequence.
ACCESSION   NZ_GG657757
VERSION     NZ_GG657757.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces viridochromogenes DSM 40736
  ORGANISM  Streptomyces viridochromogenes DSM 40736
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8653262)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Annotation of Streptomyces viridochromogenes strain DSM 40736
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8653262)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q.,
            Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z.,
            Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A.,
            Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L.,
            Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S.,
            Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 8653262)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q.,
            Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z.,
            Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A.,
            Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L.,
            Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S.,
            Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Arachne v. April 2007
            Genome Coverage                   :: 8x
            Sequencing Technology             :: ABI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000158955.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 10:17:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,837
            CDSs (total)                      :: 7,762
            Genes (coding)                    :: 7,256
            CDSs (with protein)               :: 7,256
            Genes (RNA)                       :: 75
            rRNAs                             :: 6 (5S)
            complete rRNAs                    :: 6 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 506
            CDSs (without protein)            :: 506
            Pseudo Genes (ambiguous residues) :: 0 of 506
            Pseudo Genes (frameshifted)       :: 293 of 506
            Pseudo Genes (incomplete)         :: 267 of 506
            Pseudo Genes (internal stop)      :: 21 of 506
            Pseudo Genes (multiple problems)  :: 72 of 506
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:49:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6692890
            Orig. end                         :: 6703289
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GG657757.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation was added to the scaffolds in August 2010.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(989..1003)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(4105..4983)
                     /gene="thpD"
                     /locus_tag="SSQG_RS29450"
                     /old_locus_tag="SSQG_05961"
     CDS             complement(4105..4983)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037841.1"
                     /locus_tag="SSQG_RS29450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSQG_05961"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_003993571.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-34, bitscore: 119.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATTTAVQDLYPTRGPAEVITPRRDPVVWGPTSPGLESFERDGF
                     LTDGHLLTDDETETYRTELDRLLHDPGLRTDERRIVEPRTQEVRSVFEVHKISEVFANL
                     VRDERVAGTARRILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPRMRTVSV
                     SIALTENFATNGSLMIMPGSHRTFVGCAGRTPRDNYKKSLQMQDAGTPSDEALTRFADE
                     HGIRLFTGAAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAVEPFAAPVRRPE
                     FIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="SSQG_RS29455"
                     /old_locus_tag="SSQG_05962"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958948.1"
                     /locus_tag="SSQG_RS29455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSQG_05962"
                     /product="ectoine synthase"
                     /protein_id="WP_003993572.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.4e-50, bitscore:
                     169.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSEIENTERHVRAASGTWESKRLVLARDKAGFSLHETVLY
                     AGTETSMRYAHHVEAVLCVAGEAELTDEETGETHWIKPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGWEDHDENGSYPPPEPNA"
     gene            complement(5396..6664)
                     /gene="ectB"
                     /locus_tag="SSQG_RS29460"
                     /old_locus_tag="SSQG_05963"
     CDS             complement(5396..6664)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 4.1e-72.
                     Score: 235.0. Matches aSDomain:
                     nrpspksdomains_SSQG_RS29460_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835756.1"
                     /locus_tag="SSQG_RS29460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSQG_05963"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_003993573.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.6e-74, bitscore:
                     249.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKRQPDLSVFESLESEVRSYCRGWPTVFVHARGSRLRDEHDRTY
                     VDFFAGAGSLNYGHNDPALKRALLDYIEADGITHGLDMATTAKRRFLETFRNLVLLPRE
                     LPYKVMFPGPAGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGALAVTGNVVKRAGA
                     GVPLVHGTPMPFDHYLDDRVPDFLWFERLLDDQGSGLNKPAAVIVETVQGEGGVNVARG
                     EWLRELADLCHRHDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSISGYGLPMS
                     LCLFGPGLDVWEPGEHNGTFRGNNHAFVTATAALETYWTDNRMEKQTITRGEQVEQALH
                     TIRDEHPRLGAAVRGRGLVWGIEFTEKHRASAVARRAFELGLLVETSGPEDEVVKLLPA
                     LTIPPEELDEGLRILARSVRETT"
     aSDomain        complement(5570..6577)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SSQG_RS29460_Aminotran_3.1"
                     /evalue="4.10E-72"
                     /label="SSQG_RS29460_Aminotran_3.1"
                     /locus_tag="SSQG_RS29460"
                     /protein_end="365"
                     /protein_start="29"
                     /score="235.0"
                     /tool="antismash"
                     /translation="VHARGSRLRDEHDRTYVDFFAGAGSLNYGHNDPALKRALLDYIEA
                     DGITHGLDMATTAKRRFLETFRNLVLLPRELPYKVMFPGPAGTNAVEAALKLARKVKGR
                     EAIVSFTNAFHGMSLGALAVTGNVVKRAGAGVPLVHGTPMPFDHYLDDRVPDFLWFERL
                     LDDQGSGLNKPAAVIVETVQGEGGVNVARGEWLRELADLCHRHDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTLSKSISGYGLPMSLCLFGPGLDVWEPGEHNGTFRGNNHAFVT
                     ATAALETYWTDNRMEKQTITRGEQVEQALHTIRDEHPRLGAAVRGRGLVWGIEFT"
     gene            complement(6723..7217)
                     /gene="ectA"
                     /locus_tag="SSQG_RS29465"
                     /old_locus_tag="SSQG_05964"
     CDS             complement(6723..7217)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157665.1"
                     /locus_tag="SSQG_RS29465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSQG_05964"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_003993574.1"
                     /transl_table=11
                     /translation="MTTLTEYIGEPTIADGPAVWRIARESGELDVNSPYSYLLWFRDFA
                     HTSAVARDGNGAPMGFTTGYRRPDRPDVLVIWQIAVTGADRGRGLAGAMLDHLTGRLCA
                     QSVIDRVETTISPDNTASQQLFLSFAERHRAPLKRELLFPAELFPEAHEPEYLYRIGPL
                     R"
     misc_feature    complement(7222..7235)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.8"
                     /tool="antismash"
     misc_feature    7282..7299
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.45"
                     /tool="antismash"
     gene            7440..8756
                     /locus_tag="SSQG_RS29470"
                     /old_locus_tag="SSQG_05965"
     CDS             7440..8756
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039008.1"
                     /locus_tag="SSQG_RS29470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSQG_05965"
                     /product="bifunctional cobalt-precorrin-7
                     (C(5))-methyltransferase/cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="WP_039933874.1"
                     /sec_met_domain="TP_methylase (E-value: 1.9e-19, bitscore:
                     69.3, seeds: 87, tool: rule-based-clusters)"
                     /sec_met_domain="PCMT (E-value: 7.5e-07, bitscore: 27.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPAPPAPTATVTVVGIGADGWAGLPGVSRAALSEAEVLIGGARQ
                     LDLLPPRCTGQRVAWPSPLRPAVPGLLAAHAGRRIAVLASGDPMFYGIGRALAEETDAL
                     RVLPHPSSVSYAAARLGWPLEDVEVVTLVGRPAARLAAALHHGRRLLVLSADATTPGEV
                     AALLRDRGFGPSRMRVLEQLGGEKEHATAESTADDWQHPPGDPLNIVAVDCRRAPDTLR
                     LGAVPGLPDEAYEHDGQLTKRHVRAATLGVLAPAPGELLWDIGGGSGSIAVEWMRTHPS
                     CRAVTVERDPARAERITRNADRLGVPGLRVVTGAAPAVLAELPPPDAMFVGGGLTAPGL
                     LDACWEALPDGGRLVANTVTLESEALLADAHRRHGGELVRLAVSHAVPVGGFTGWRQAM
                     PVTQWAVRKTLGTAGLGAPGLGTAGPATPGLDTLSGADR"
     gene            8753..9502
                     /gene="cobM"
                     /locus_tag="SSQG_RS29475"
                     /old_locus_tag="SSQG_05966"
     CDS             8753..9502
                     /EC_number="2.1.1.133"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cobM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409267.1"
                     /locus_tag="SSQG_RS29475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSQG_05966"
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="WP_003993576.1"
                     /sec_met_domain="TP_methylase (E-value: 6.6e-43, bitscore:
                     145.9, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVYFIGAGPGAADLITVRGARTLAACRVCLYAGSLVPRELLAEC
                     PPDARLVDTAQLDLDQITAELLRAHEEGHDVARLHSGDPSVFSAVAEQMRRLDAAGVPY
                     EVVPGVPAFAAAAASLKRELTVPTVGQSVILTRVANRATAMPEGEDLATLGRSGALIVL
                     HLAARYADRVVEELLPHYGADCPAAVVAYASRPDEIVIRGTLEEIAGKVKEAGVLRTAV
                     IMVGRTLGAEQFRDSHLYSPERERHVC"
     misc_feature    9559..9572
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
ORIGIN
        1 aggaagatca cctacggagc gcgcttcagc aaggcgaccg cctcggtgat cgaccgcatc
       61 gcccgccgcg cgctggacgt tcctcccgac ctggaacacg ttcccgtgac cctgctggcg
      121 gaggagatcg acgccgaact ggagcgcatc gacgccgccg agggcatgcc gaagcaggac
      181 gtggtgtgca ccaagcacga cgaggcctcc cagccaccgg cgccgttcct ccgcaacccg
      241 caccacgccg tcgacgccgc ggagcagttc cgggccgaag cggtcgccgc tctgcggcgc
      301 tacgccgaaa aactggatcc ccggctggac ctgcaccact tcgccgcccg ggccgccggc
      361 accccggacg cggaggcgtt cttcttcagc gggcgccgta ccgagctcaa gcgcatcgag
      421 gagtggctcg gcgattgcga cccccggcgg aaccgcctcc tggccgtcat cggcagaccc
      481 ggagcgggca agtcggccct cctcggggtg acggtgtgca ccgtccaccc ccagctgcgt
      541 cccctgcggg accggctcga cctgcgtgat ttccgccccc cgcccgatcc ccggctgctg
      601 gccgtgcacg cgcaccacct gtccacccag gacgtcgtgg actcgctgca ccggcaggtg
      661 agggcactgc gcacgcccag gcaactgggc ggacgtccgc ctgcgcgggc ggaggcgacg
      721 aacgcgaccc ccgaggaact ggccgcggct ctgcgcgcgg tcggcgacgt caccgtcgta
      781 ctcgacgcgc tcgacgaggc cgacgacccc gacgggctgc tgcggcacgt gctgctgccg
      841 ctcgcgggcg agggcggcga tccggtgccc ggctgccggg tgctggtcgg tacccggctg
      901 tggccggaca gcctgcccga cctgcatgcg gccgtcgccg gccaccccga tcgactcctc
      961 gacctcgacg cggaacgacc cgcgtcactg gccgagcacc tcgccaccta cctgggccgg
     1021 ctgctggaca ccgattaccc cgccgacgtc acggagatga tcgcgcgccg cctgtcgggc
     1081 cccgccgagc acggggcgtt cctgaccgcg gccgtgtacg gcgcccacct gcgcagactc
     1141 tcccgggcgg gcacactcct ctcccgggac gagatcgccc gggacctgcc gtgcgatctg
     1201 ccggggatgc tcgaactgaa cctgcgcacg ctcaccgccg aactgccctg gaccggcgcg
     1261 gtgctggcgg cgctcgcccg ggcggccggg cagggcatgc cgtccggcct cgtgcaccag
     1321 gtggccctcg cgctgcgccc cgacgcccgt gacggggcgc tgccgccgcg gctgagggac
     1381 acggccgagg ctctcaccga ggcgcggttc tacctccggt ccacccacga ccccggtgcc
     1441 gatccggccc tggacggcgg gaagctctac cgcttcttcc accgggcgcc ggccgagtac
     1501 ctggcggagc gcacggatcc cggagccgta ctgcgggccc tgctgtcgac cgtgccggcc
     1561 gagcacggtg tccgcgactg gtccagggcg gaccgttatc tgcggcgaca cgcggccgac
     1621 cacgcggtgg acgccggccc ggaccaactg gacgcgctgc tggaggacgt gtcgtacctg
     1681 ctgcacgcgg acccggagcg gctcgtacgg cacctgcacc gcgcccgcgg tcaccgggcc
     1741 cggctgcacg ccgacgtcta ccggcagacc acggcacacc atccgctccg gcacatcacg
     1801 gccgtacgac gggacctgct gtgcctcgac gcgaccgtgt ggcgggacac cgggctgtcc
     1861 gacacgctgg cctcccagcc gggcctgccc ccggcaccga cccggccggt ctgggcggcg
     1921 agccggacgg cgaccacggc acggctgcag accctcggcc cgcatgccgg gccggtctcc
     1981 cgggtgatgc tgactcaggt gccggacgac gagggggccg gggtatcgga gtgcggcggg
     2041 acgtacgcgg tcgtcagcac cggggacctc gaccggacac ggttgtcgga cccgttcagc
     2101 ggcaggaccc tgcgcaccct cggcggtctg tcggggccgg tccgggcgat cgcgccggcc
     2161 cggctcgcgg acggccgccc cgtcctcgtc tccggaggtg aggacggtca cttgtacgtc
     2221 cacgacctgc gcaccggcga ccgggtccgt cggcgggcgg ccggcatcgg gccgatccgg
     2281 acgctcgtca ccgtcaccct cccggagggc ggcacccgcg cggtggccgc cgggcagggc
     2341 aagacgctgc tcctgtggaa cccggacgac ggccgcctga cccggctgga cagctctctg
     2401 gtgctgatct ccggactggc cgccacgcga ctgccggacg gcaccccggt cgccgtcgcc
     2461 gtcggtgagg agcaggcggc ccgggtctgg gacgtgcgga cgggacagcc gctgggcacc
     2521 ctcggaccgc acgccgactt cgtccgcgcg gtggccacga ccacgctggg cgacggcacg
     2581 gtcgtggccg tcaccggctg ctacgacggc cgtgtgctgg tgtggaacgc ggcggacggg
     2641 cgcaggccgc acgtgctgac ggggcacgcg gaccgggtga cccgcgtggc cgcgggtacc
     2701 gccggcgacc ggcacgtcgc ggtgagcggt gaccgcgcgg ggcgtgtcct ggtgtgggac
     2761 ctcgccgcgg gccggcggct gcacgagctg gtgaccgcgg gccacggggt gatcacctcc
     2821 ctcgcgctgg tcgaggacgg cgggggcgga cgggtcgtca cgggctgcgc cgacggacga
     2881 gtcgccgtgt gggaactcgg caccggacgg cggacccacg tcttcgccgc gcatcccggt
     2941 ggcgtgcaca gcgtcgacgt ggcgctgatc gacgagcggc tggtggcggt gagcggcggc
     3001 gccgacggca acgccgtcgt ctgggacgtc gtggacgccc gccccgacgc cccggagacc
     3061 gggcacgagg aggtgatgga ggcggtggcc atcgccgcgc cggaggagcg gccggccctg
     3121 tgcgtcgccg ggggcggcga gcggggtgcg gaggtgcggg acctggcgac gggcgaccac
     3181 cgacacgtgc tggacgactc ggggcgccgg ttgctggtgc gggcgctggc gacggccgac
     3241 gtgccggacc tggggccggt cgtcgtggcg ggcggccggg acggttcact gcggatgtgg
     3301 gagctgacga ccggaaagct ggtccacagg tttcccggcg ggccgggacg gatcagggcg
     3361 gtggccgtgg gccgcaccgc ggacggccgg caggtcctgg tcgccggcgg agacacggcc
     3421 gtcgcgaccg tgtgggagct gcggacgggg acgcggttgc cgaccttcac cgggcacggc
     3481 ggccgggtga cggcgatatc cctggccgac ggtggccgca cccccggcct cgtggtcagc
     3541 ggtgaccgca ggggctccct gctggtgtgg gacgtgtcga ccgggtccga agtggcggcg
     3601 ctgacccgcg gcgaccggcg catcgaggcg gtggccaccg ccgtgctgcc cggggaacgg
     3661 acggtggccg tgtccggtgg ggacgggggc gcggtccgcg tgtgggacct ggaccgtccg
     3721 ggggtgtccg aacctctcac cggcctgcgg cagcgggtga ccgccctggc cacggtgcgg
     3781 ctgcccggag gccgagtcct cgccctcggc gggggcgagg accggcgtgt cgtcctctgg
     3841 gatctcgtca cccggcagca gctggcggcg ccttaccacc tgcccaccgc cgtctcctcg
     3901 atcgccgcct gtgccacggg attcgtcgtc gcgcacgaag cggggacggc gagcttcgcc
     3961 tggtgcgcgg aactcctcac cccggtgtcc ggaggagagg ggtccccggc cccgtcgccc
     4021 tgatctcacc gcaccggcgt gaagtcccgc gccccgatgc tgtgttctcc gggggcgacc
     4081 cccgggcccc cggcaggggg ttggtcaccg caccggcgtg aagtcccgcg ccccgatgaa
     4141 ctccggccgc cgcaccggcg ccgcgaacgg ttccaccgcc gtgttctcca cgctgttgaa
     4201 gacgatgaag acgttgctgc gcggatacgg cgtgatgttg tcgccggaac cgtgcatgca
     4261 gttgcagtcg aaccaggtcg ccgagcccgc cgcgccggtg aacaggcgga tgccgtgctc
     4321 gtcggcgaag cgggtcagcg cctcgtcgga cggtgtgccc gcgtcctgca tctggaggga
     4381 cttcttgtag ttgtccctcg gcgtccggcc ggcgcagccg acgaacgttc ggtgcgagcc
     4441 cggcatgatc atgaggctgc cattggtggc gaagttctcg gtcagcgcga tcgagaccga
     4501 caccgtgcgc atccgcggca ggccgtcctc ggcgtgccag gtctcgaagt ccgagtgcca
     4561 gtagaacccc gaagcgccga agcccggctt gacattgatc cgggactggt ggacgtacac
     4621 gtcggagccg aggatgcggc gggcggtgcc cgccacgcgt tcgtcgcgta ccaggttcgc
     4681 gaagacctca ctgatcttgt gcacctcgaa caccgaccgg acttcctggg tcctgggttc
     4741 gacgatgcgg cgttcgtcgg tgcggagccc cgggtcgtgg agcagccggt cgagttcggt
     4801 gcggtaggtc tcggtctcgt cgtcggtcag caggtggccg tcggtcagga agccgtcgcg
     4861 ttcgaacgac tccagaccgg gggaggtcgg cccccagacg accggatccc tgcggggtgt
     4921 gatgacctcc gcggggcccc gggtggggta caggtcctgg acggccgtgg tggtcgcggt
     4981 cattggccgg tccccttccg tcaggcgttg ggttcggggg gcgggtagct gccgttctcg
     5041 tcgtggtcct cccacccggt gaccgggggg ttgaagacgc acacgcagcg gaagtccgtc
     5101 ttggggcgga gcgtgtgccg ctcgtggccg tcgagcaggt acatcgtgcc gggcttgatc
     5161 cagtgggtct cgcccgtctc ctcgtcggtg agttcggcct cgcccgcgac gcacagcacg
     5221 gcctcgacgt ggtgcgcgta ccgcatcgac gtctccgtgc ccgcgtacag cacggtctcg
     5281 tgcagcgaga agccggcctt gtccctggcg agcacgaggc gtttgctctc ccaggtaccg
     5341 gaagcggccc tcacatggcg ctcggtgttc tcgatctcgc tcagtgaccg gactatcacg
     5401 tcgtctcccg taccgaccgg gcgaggatcc gcagcccctc gtcgagttcc tccggaggga
     5461 tggtgagtgc cggcagcagc ttgacgacct cgtcctcggg cccggacgtc tcgaccagca
     5521 gcccgagttc gaaggcgcgg cgggccacgg ccgacgcgcg gtgcttctcg gtgaactcga
     5581 tcccccacac cagtccgcgt ccgcggacgg cggcgccgag ccgggggtgc tcgtcgcgga
     5641 tcgtgtgcag cgcctgctcg acctgttccc cgcgcgtgat ggtctgcttc tccatccggt
     5701 tgtcggtcca gtacgtctcg agcgcggcgg tcgcggtgac gaaggcgtgg ttgttgccgc
     5761 ggaaggtgcc gttgtgctca cccggttccc acacgtccag gccgggcccg aacaggcaca
     5821 gcgacatcgg caggccgtag ccgctgatgg acttcgacag cgtgacgatg tccggcgtga
     5881 tgccggcctc ctcgaaggag aagaaggccc cggtgcgtcc gcagcccatc tggatgtcgt
     5941 ccacgatgag cagcatgtcg tgccggtgac agagatcggc cagctcccgc agccactcgc
     6001 cccgggccac gttgacgccg ccctcgccct gcaccgtctc gacgatcacc gcggcgggct
     6061 tgttcagacc cgagccctgg tcgtcgagca gccgctcgaa ccacaggaag tccggcaccc
     6121 ggtcgtcgag gtagtggtcg aacggcatgg gggtgccgtg caccagcggg acgccggcgc
     6181 cggcccgctt gacgacgttg ccggtgacgg ccagcgcgcc cagggacatg ccgtggaacg
     6241 cgttggtgaa ggagacgatc gcctcgcgcc ccttcacctt gcgggccagc ttcagcgcgg
     6301 cctccacggc gttggttccg gccgggcccg ggaacatcac cttgtacggc agttcgcgcg
     6361 ggagcagcac cagattccgg aaggtctcca ggaacctgcg cttcgcggtg gtggccatgt
     6421 ccaggccgtg ggtgattccg tcggcctcga tgtagtcgag gagggcccgc ttgagagcgg
     6481 ggtcgttgtg gccgtagttg agcgatccgg cacccgcgaa gaagtcgacg taggtgcggt
     6541 cgtgctcgtc gcgaaggcgg cttccccgcg cgtggacgaa gacggtgggc caccctcggc
     6601 agtaactgcg tacctcggat tcgaggcttt cgaatacgct cagatccggc tgccttttcg
     6661 tcatgacact cctaggtgcg ggaagagcct gagcaccggc acttctgcgg cgccgccgaa
     6721 tttcagcgca gtggtccgat gcggtacagg tactcgggct cgtgggcctc gggaaacagc
     6781 tccgcgggaa agaggagttc gcgtttcaac ggtgccctgt gtcgttcggc gaaggagagg
     6841 aacagctgct gggaggcggt gttgtccggg ctgatggtcg tctccacccg gtcgatgacg
     6901 ctctgcgcac acagtcggcc ggtgaggtgg tcgagcatgg cgccggcgag gccccgtccg
     6961 cgatcggccc cggtgacggc gatctgccag atgacgagca cgtcgggccg gtcggggcgg
     7021 cggtagccgg tggtgaaacc catcggtgcg ccgtttccgt cgcgagcgac cgcggaagtg
     7081 tgcgcgaaat cgcggaacca cagcaggtaa ctgtacggag agttgacgtc gagttctccg
     7141 gattcgcggg cgatacgcca gacagcggga ccgtccgcga ttgttggttc cccgatgtat
     7201 tccgtcagtg tcgtcatacg gagcaccttt gttccacggt caccgggtgt taccgcattc
     7261 ccggggcgtg tgcgactact catgttacgt gggtggtccc gactcggcca cgggaccaat
     7321 acttgaaacc ccaaccgtga tcggggagag ggcggcgacg gcccacccga gcgaggatgc
     7381 gctggtcacc gcccagtaga gtgcgcctct gtcgtccccg aaagccgagg taccgcgcag
     7441 tgacgcccgc accccccgca ccgaccgcca ccgtgaccgt cgtcgggatc ggggccgacg
     7501 gctgggcggg gctgcccggc gtctcccgtg ccgcgctgag cgaggccgag gtcctgatcg
     7561 ggggcgcgcg ccagctggat ctgctgccgc cgcggtgcac cggacagcgc gtcgcctggc
     7621 cgtcgccgct gcgccccgcc gtccccggcc tgctcgccgc gcacgccggg cgccggatcg
     7681 ccgtgctggc cagcggagac cccatgttct acggcatcgg acgcgccctc gccgaggaga
     7741 cggacgccct gcgcgtcctg ccgcacccct cctcggtctc ctacgccgcg gcccggctcg
     7801 gctggcccct ggaggacgtg gaggtcgtca cgctcgtcgg ccggcccgcc gcccgcctcg
     7861 ccgccgccct gcaccacggc cggcgcctcc tcgtcctcag cgcggacgcc accacccccg
     7921 gcgaggtcgc cgccctgctg cgggaccggg gcttcggtcc cagccggatg cgggtcctgg
     7981 agcaactggg cggcgagaag gaacacgcca ccgccgagtc caccgccgac gactggcagc
     8041 acccgcccgg cgaccccctc aacatcgtcg ccgtcgactg ccgccgcgcc cccgacaccc
     8101 tgcggctcgg cgccgtgccc gggctgccgg acgaggcgta cgagcacgac gggcagctca
     8161 ccaagcggca cgtccgcgcc gccaccctcg gggttctcgc gcccgcgccc ggtgaactgc
     8221 tgtgggacat cggcggtggt tccggatcca tcgccgtcga gtggatgcgc acccacccct
     8281 cgtgccgcgc ggtcaccgtg gagcgcgatc cggcgcgcgc cgagcggatc acgcgcaacg
     8341 cggaccggct cggtgtgccc ggcctgcggg tcgtcaccgg tgccgccccc gccgtcctcg
     8401 ccgagctccc gccccccgac gcgatgttcg tcggcggcgg actcaccgcg cccggcctcc
     8461 tcgacgcgtg ctgggaggcg ctgcccgacg gcgggcggct ggtcgccaac accgtgacgc
     8521 tggagtccga ggcgctgctc gccgacgccc accggcgaca cggcggcgag ctggtgcggc
     8581 tcgcggtttc gcacgccgtg ccggtggggg gcttcaccgg gtggcggcag gcgatgccgg
     8641 tgacccagtg ggccgtgcgg aaaaccctcg gcaccgcggg cctcggcgcc ccaggactcg
     8701 gcaccgcagg accagccacc ccaggactcg acaccctttc gggagcagac agatgaccgt
     8761 gtacttcatc ggtgcgggcc ccggtgccgc cgacctgatc acggtgcgcg gcgcccgcac
     8821 gctcgccgcc tgccgggtct gcctgtacgc gggcagcctg gtcccgcgtg aactgctcgc
     8881 cgaatgcccg ccggacgccc ggctggtgga caccgcgcag ctcgacctgg accagatcac
     8941 cgccgaactg ctgcgcgccc acgaggaggg gcacgacgtg gcccggctgc actccgggga
     9001 cccgtccgtg ttcagcgcgg tggccgagca gatgcggcgg ctggacgcgg ccggggtgcc
     9061 ctacgaggtc gtcccgggcg tcccggcctt cgccgccgcg gcggcctccc tgaagcggga
     9121 gctgaccgtg ccgaccgtag gccagagcgt gatcctcacc cgtgtcgcca accgggccac
     9181 cgccatgccc gagggcgagg acctcgccac gctcggccgc agcggcgcgc tgatcgtgct
     9241 gcacctggcc gccaggtacg cggaccgcgt ggtcgaggag ctcctcccgc attacggcgc
     9301 ggactgcccc gccgcggtcg tcgcctacgc ctcccgcccc gacgagatcg tcatccgcgg
     9361 cacgctggag gagatcgccg ggaaggtgaa ggaggccggt gtgctgcgca ccgccgtgat
     9421 catggtgggg cggacgctgg gagcggagca gttccgggac agccacctgt actcgccgga
     9481 gcgggaacgc cacgtgtgct gagcacggtg agcggccgtc aggccaccgc gctgcgcccc
     9541 accaccgtcc ccgcccggtc gatgcagatc acgtcgaccg ccaccggagc cccccgcagc
     9601 acggccaggg cctcgtcgcg ggcggtggtg gcgaccaggt cgccgagcgg caccccggcc
     9661 gcgatgcagg actggagcgc ggcgaggccg gtgttggccc cggccacctc ggcggccagg
     9721 gcctcgtccg cgccgccgcg ccgggccagt tcggcgagga agcccttgtc gacctgggag
     9781 cgggcggagt gcaggtcgag gtggccggcg gcgagcttgg agagcttggc gaagccgccg
     9841 cagacggtca gccggtcgac ggggtggcgg cggatgtact tcagcaccgc gcccgcgaag
     9901 tcgcccatgt cgagcagggc gtcctcgggc aggccgtact cggcgacgac cgtcttctcc
     9961 gaggtcgacc cggtgcagcc cgcgacatgc gtacggcccg ccgcccgggc cacgtccaca
    10021 ccccgccgaa tggagtcgat ccacgccgag caggagtacg gcacgacgat gccggtcgtg
    10081 cccaggatgg agaggccgcc caggatgccg agccgggggt tccaggtgga gcgggcgatc
    10141 tcctcgccgt ggtccacgga gacggtgatc tcgacgtccc cggtgccgcc gtgccgggcg
    10201 gcgacctcgg cgacgtggtc gcccatcatg cggcggggga cggggttgac ggccgcctcg
    10261 ccgacgggca ggggcaggcc ggggcgggtg atggtgccga ccccgggccc cgccctgaac
    10321 acgaccccgg ccccggcggg cagcggccgt accgtggccc ggaccagggc cccgtgggtg
    10381 acgtccggat cgtcgcccg
//
