LOCUS NZ_GG657754 10405 bp DNA linear CON 01-MAY-2024 DEFINITION Streptomyces himastatinicus ATCC 53653 supercont1.1, whole genome shotgun sequence. ACCESSION NZ_GG657754 VERSION NZ_GG657754.1 KEYWORDS WGS; RefSeq. SOURCE Streptomyces himastatinicus ATCC 53653 ORGANISM Streptomyces himastatinicus ATCC 53653 Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group. REFERENCE 1 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D., Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Annotation of Streptomyces hygroscopicus strain ATCC 53653 JOURNAL Unpublished REFERENCE 2 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Direct Submission JOURNAL Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA REFERENCE 3 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Direct Submission JOURNAL Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: Arachne v. April 2007 Genome Coverage :: 6x Sequencing Technology :: ABI ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_000158915.1-RS_2024_05_01 Annotation Date :: 05/01/2024 03:02:38 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 9,680 CDSs (total) :: 9,608 Genes (coding) :: 8,720 CDSs (with protein) :: 8,720 Genes (RNA) :: 72 rRNAs :: 4, 1 (5S, 16S) complete rRNAs :: 4, 1 (5S, 16S) tRNAs :: 64 ncRNAs :: 3 Pseudo Genes (total) :: 888 CDSs (without protein) :: 888 Pseudo Genes (ambiguous residues) :: 0 of 888 Pseudo Genes (frameshifted) :: 423 of 888 Pseudo Genes (incomplete) :: 530 of 888 Pseudo Genes (internal stop) :: 36 of 888 Pseudo Genes (multiple problems) :: 97 of 888 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 20:01:42 NOTE :: This is a single region extracted from a larger record! Orig. start :: 8099141 Orig. end :: 8109546 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to GG657754.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation was added to the scaffolds in August 2010. FEATURES Location/Qualifiers region 1..10405 /candidate_cluster_numbers="1" /contig_edge="False" /product="ectoine" /region_number="28" /rules="ectoine_synt" /tool="antismash" cand_cluster 1..10405 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="ectoine_synt" /kind="single" /product="ectoine" /protoclusters="1" /tool="antismash" protocluster 1..10405 /aStool="rule-based-clusters" /category="other" /contig_edge="False" /core_location="[5000:5405](-)" /cutoff="20000" /detection_rule="ectoine_synt" /neighbourhood="5000" /product="ectoine" /protocluster_number="1" /tool="antismash" proto_core complement(5001..5405) /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="ectoine_synt" /neighbourhood="5000" /product="ectoine" /protocluster_number="1" gene complement(162..932) /locus_tag="SSOG_RS34540" /old_locus_tag="SSOG_06823" CDS complement(162..932) /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1195: IclR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007381154.1" /locus_tag="SSOG_RS34540" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06823" /product="IclR family transcriptional regulator" /protein_id="WP_009718909.1" /transl_table=11 /translation="MTAAETGGAQVKSAVRTVELLEFFAGRPGMHSLASVQEAVGYPKS SLYMLLRTLVELGWVETDATGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTT ETIHLARLDGTNVVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYTDEQVRKLL PETLSPLTEHTITDREKLIEELHLIREQGYAVDREENTLGLRCFGIAIPYRTPSRDAIS CSVPVARLTGAHEQMIKDALFDARDRLTLATRRL" gene 1019..2599 /locus_tag="SSOG_RS34545" /old_locus_tag="SSOG_06824" CDS 1019..2599 /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Aldedh" /gene_functions="biosynthetic-additional (smcogs) SMCOG1017: aldehyde dehydrogenase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014059810.1" /locus_tag="SSOG_RS34545" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06824" /product="aldehyde dehydrogenase (NADP(+))" /protein_id="WP_078510655.1" /sec_met_domain="Aldedh (E-value: 7.2e-42, bitscore: 142.6, seeds: 89, tool: rule-based-clusters)" /transl_table=11 /translation="MCETQAAVTQQEESAVSAPVWSVDPRTGKQREQVAVEATAEEVDK VVRAADAARGALTDRTVRAAFLRTAADLLDEARDTLVEAADAETALGPARLTGELARTS YQLRSFATIVDEGAFLDVHVDHADATQTPPWPDLRRYKLPLGVVAVYAASNFPLAFSVP GGDTASALAAGCPVVVKAHPDHPATSEVAASALRRAAEQVGLPADVISVVHGFDAGLEL IKHPLIAAAGFTGSIRGGRALYDAAAARPVPIPFHGELGSLNPVVVTEAAAAERGEQIG SGLSGSMTLGVGQFCTKPGFVLAPAGAAGDALVKSLTEAVSNTDAGVLLDHRMRDNFVA GVKERAELADVEAPVTPGSGGEHSVSPGFLTVPARRLAEQGPHDVLLEECFGPVTVVAR YESDAEISAVLGRLQGNLTATVQISAAEAEGTEGRAGELIAELTPLAGRVLVNGWPTGV AVAPAQHHGGPYPATTSTSTSVGGTAVERWLRPVSYQDTPPALLPPELRDDNPLGLPRR VNGIREQQG" misc_feature 2785..2798 /note="TFBS match to ArgR, Regulator of arginine biosynthesis genes, confidence: weak, score: 16.85" /tool="antismash" gene 2794..3846 /locus_tag="SSOG_RS34550" /old_locus_tag="SSOG_06825" CDS 2794..3846 /NRPS_PKS="Domain: Aminotran_5 (13-342). E-value: 3.3e-23. Score: 74.1. Matches aSDomain: nrpspksdomains_SSOG_RS34550_Aminotran_5.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_5" /gene_functions="biosynthetic-additional (smcogs) SMCOG1139: aminotransferase class V" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007448151.1" /locus_tag="SSOG_RS34550" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06825" /product="aminotransferase class V-fold PLP-dependent enzyme" /protein_id="WP_106516700.1" /sec_met_domain="Aminotran_5 (E-value: 7.3e-17, bitscore: 60.4, seeds: 34, tool: rule-based-clusters)" /transl_table=11 /translation="MESLAGAEFAPQTTYLNTASSGLIPARSTAAMKAALDDAAVGVRY TDAAFEAVEETRAAYARLVGVPARRVATGGSVVVYAGLIATSLPSGAEVLTAEGDFSSL VNPFAVRRDLKLRTVPLEELAEAVRPETALIAVSAVQSADGRIADLDAIRDAARAHGAR TLVDLSQAVGWLPVSAGEDDFTVAVGYKWLLCPHGTAFLTVPEDLGGLTPVFAGWRSGE QPWDSCYGPVAEPARSARRYDESPALLAYVAARHSLALLHELGTEAVRAHDRALADRFR AGITGLGRSCVPAPGSVIVSVPGLGEAAPRLAKADVQVSARAGNLRAAFHLYNTPADVD RLLEVLGDGA" aSDomain 2833..3819 /aSDomain="Aminotran_5" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_SSOG_RS34550_Aminotran_5.1" /evalue="3.30E-23" /label="SSOG_RS34550_Aminotran_5.1" /locus_tag="SSOG_RS34550" /protein_end="342" /protein_start="13" /score="74.1" /tool="antismash" /translation="TYLNTASSGLIPARSTAAMKAALDDAAVGVRYTDAAFEAVEETRA AYARLVGVPARRVATGGSVVVYAGLIATSLPSGAEVLTAEGDFSSLVNPFAVRRDLKLR TVPLEELAEAVRPETALIAVSAVQSADGRIADLDAIRDAARAHGARTLVDLSQAVGWLP VSAGEDDFTVAVGYKWLLCPHGTAFLTVPEDLGGLTPVFAGWRSGEQPWDSCYGPVAEP ARSARRYDESPALLAYVAARHSLALLHELGTEAVRAHDRALADRFRAGITGLGRSCVPA PGSVIVSVPGLGEAAPRLAKADVQVSARAGNLRAAFHLYNTPADVDRL" gap 3871..3970 /estimated_length=unknown gene complement(4109..4996) /gene="thpD" /locus_tag="SSOG_RS34555" /old_locus_tag="SSOG_06826" CDS complement(4109..4996) /EC_number="1.14.11.55" /codon_start=1 /gene="thpD" /gene_functions="biosynthetic-additional (rule-based-clusters) PhyH" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009718912.1" /locus_tag="SSOG_RS34555" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06826" /product="ectoine hydroxylase" /protein_id="WP_009718912.1" /sec_met_domain="PhyH (E-value: 9.5e-38, bitscore: 129.7, seeds: 39, tool: rule-based-clusters)" /transl_table=11 /translation="MTTVTDLYPTRGPLEVATPRVDPVVWSDPGAEGPMQPSELSDFDR DGFLAIEQLITPDEVAVYRAELDRLIVDPDVRADERSIIEPKSQDVRSVFEVHRISEVF ANLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT VSVSVALTENFDTNGGLMIMPGSHKTFLGCAGQTPKDNYKKSLQMQDAGIPSDEALSGF ADKHGIKLFTGKAGSATWFDCNCMHGSGDNITPYSRSNVFIVFNSVENTAVEPFAAPVR RPEHIGARDFTPVR" gene complement(5001..5405) /locus_tag="SSOG_RS34560" /old_locus_tag="SSOG_06827" CDS complement(5001..5405) /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) ectoine: ectoine_synt" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009718913.1" /locus_tag="SSOG_RS34560" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06827" /product="ectoine synthase" /protein_id="WP_009718913.1" /sec_met_domain="ectoine_synt (E-value: 5.4e-53, bitscore: 177.3, seeds: 10, tool: rule-based-clusters)" /transl_table=11 /translation="MIVRSFKDIEGTERHVKAKSGTWESKRIVLAKEKVGFSLHETTLY AGTETSMWYANHIEAVLCVEGEAELTNDENGEKHIITPGTMYLLDGHEKHTMRIKKDFR CVCVFNPPITGREDHDENGVYPLITETEEA" misc_feature complement(5423..5439) /note="TFBS match to HexR, Glucose-responsive regulator, confidence: weak, score: 16.6" /tool="antismash" gene complement(5447..6709) /gene="ectB" /locus_tag="SSOG_RS34565" /old_locus_tag="SSOG_06828" CDS complement(5447..6709) /EC_number="2.6.1.76" /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate transaminase activity [Evidence IEA]" /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 3e-73. Score: 238.7. Matches aSDomain: nrpspksdomains_SSOG_RS34565_Aminotran_3.1" /codon_start=1 /gene="ectB" /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_3" /gene_functions="biosynthetic-additional (smcogs) SMCOG1013: aminotransferase class-III" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007448159.1" /locus_tag="SSOG_RS34565" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06828" /product="diaminobutyrate--2-oxoglutarate transaminase" /protein_id="WP_009718914.1" /sec_met_domain="Aminotran_3 (E-value: 3.5e-77, bitscore: 258.9, seeds: 13, tool: rule-based-clusters)" /transl_table=11 /translation="MTITPPALSVFEALESEVRSYCRGWPAVFDRAQGSHMYDEDGHTY LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNNILRPRD LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA GIPLVHGTPMPFDNYFDGAVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA EWLRALSELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGMPLA LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEQVEQGLR AICDENSGISHRGRGLVWGMEFEDKPRASAVCKRAFELGLLVETSGPESEVVKLLPALT MTPEELDEGLRILARAVRETA" aSDomain complement(5621..6625) /aSDomain="Aminotran_3" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_SSOG_RS34565_Aminotran_3.1" /evalue="3.00E-73" /label="SSOG_RS34565_Aminotran_3.1" /locus_tag="SSOG_RS34565" /protein_end="363" /protein_start="28" /score="238.7" /tool="antismash" /translation="FDRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE RDGVTHGLDMSTTAKRAFLESFQNNILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGAVEDFLWFER LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALSELCERQDMLLIVDDIQMGCGR TGAFFSFEEAGITPDIVTVSKSISGYGMPLALTLFKPELDIWEPGEHNGTFRGNNPAFV TAAATLDTYWADGQMEKQTLARGEQVEQGLRAICDENSGISHRGRGLVWGMEFE" gene complement(6841..7389) /gene="ectA" /locus_tag="SSOG_RS34570" /old_locus_tag="SSOG_06829" CDS complement(6841..7389) /EC_number="2.3.1.178" /GO_function="GO:0033816 - diaminobutyrate acetyltransferase activity [Evidence IEA]" /GO_process="GO:0019491 - ectoine biosynthetic process [Evidence IEA]" /codon_start=1 /gene="ectA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014059805.1" /locus_tag="SSOG_RS34570" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06829" /product="diaminobutyrate acetyltransferase" /protein_id="WP_009718915.1" /transl_table=11 /translation="MTAAQADRVGARSEIDLPEGLSLDTPRVEDGAAIWRIARDSKTLD LNSSYSYLLWCRDFAATSVVARDAEGGPVGFITGYIRPDRPETLVVWQVAVDQAWRGRG LAATLLDGLTARVAATGIRGVETTITPDNTASNRLFTSFAERHGAPVEHEVLFDGGLFP DGGHEPEVLYRIGPLADRG" misc_feature complement(7547..7562) /note="TFBS match to CRP, Cyclic AMP receptor protein, confidence: weak, score: 16.03" /tool="antismash" misc_feature 7557..7572 /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2, activator of antibiotic production, confidence: medium, score: 17.62" /tool="antismash" gene complement(7760..8539) /locus_tag="SSOG_RS34575" /old_locus_tag="SSOG_06830" CDS complement(7760..8539) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014059804.1" /locus_tag="SSOG_RS34575" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06830" /product="hypothetical protein" /protein_id="WP_039939356.1" /transl_table=11 /translation="MIDHRDDRRDVAAELREAAEAHQPDRARMLARVTAGMAAGERRSG RRVPARQWTRVVGPAAGLAAAVAAAGIAVVTTDTADGPQTVRTSSEPAGPPAGEGGGTA RRPGGGAGDGAGRHTPSAYPTSVGPNHLADPAVRSRGAINPNSNPYWAQSDITVTTSKP LTSLTVELRVAENGGVHTTGSWSTLPVDDLAVSVRSEGGVLVYRWTLRKGATVPAGRHV FAGQYNHAEGSRDAGRDRYSASGNGPSGAFAVRGDFP" gene complement(8539..9234) /locus_tag="SSOG_RS34580" /old_locus_tag="SSOG_06831" CDS complement(8539..9234) /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /GO_function="GO:0016987 - sigma factor activity [Evidence IEA]" /GO_process="GO:0006352 - DNA-templated transcription initiation [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1032: RNA polymerase, sigma-24 subunit, ECF subfamily" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019433895.1" /locus_tag="SSOG_RS34580" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_06831" /product="SigE family RNA polymerase sigma factor" /protein_id="WP_078510465.1" /transl_table=11 /translation="MSAPTDGPSAAATTPTSGLPADGLPAADAQAEFREFFERHHAELA RLAHLLLGSSDGADDLAADALVAVWHRWDRVRVAEHPAAYARGVVANLARSRIRSLVRE RRRVALFSSERLDRVDDPDVSAMVDVQAALVRLPFRKRACVVLRHAFDLSERDTARTLG ISVGTVKSQTSRGIAELERLLGPPDDDTERPAAGTQAQAPPPAPSAQETVRGLRPGRVG HAGHPGGNG" misc_feature complement(9163..9165) /note="tta leucine codon, possible target for bldA regulation" /tool="antismash" misc_feature complement(9178..9180) /note="tta leucine codon, possible target for bldA regulation" /tool="antismash" misc_feature complement(9221..9235) /note="TFBS match to CelR, Cellobiose uptake repressor, confidence: weak, score: 22.04" /tool="antismash" misc_feature 9222..9236 /note="TFBS match to CelR, Cellobiose uptake repressor, confidence: weak, score: 22.04" /tool="antismash" ORIGIN 1 cgacggcgcg cagcgcccgg cggggagcgg tggtggcgtg gggctgtgtc atgtacacca 61 gccttgcgga gcgtccggct ccgcacctcg ccctgtccga cgatccgcct ccgccgtgcg 121 gcggaggccg tcgtcctccc cgcgacccgc ggccttcgga ttcagagacg gcgcgtggcg 181 agcgtgaggc ggtcgcgggc gtcgaacagc gcgtccttga tcatctgctc atgggccccg 241 gtcagccggg ccaccggcac cgagcagctg atggcgtccc gggagggggt gcggtagggg 301 atggcgatgc cgaagcagcg cagccccagg gtgttctcct cgcggtccac cgcgtacccc 361 tgctcgcgga tcaggtgcag ttcctcgatc agcttctcgc ggtcggtgat cgtgtgctcg 421 gtgagcgggc tcagcgtctc cggcagcagc ttgcgcacct gctcgtcggt gtacgtggcc 481 agcagcgcct tgcccagcga ggtggagtgg gccgggaggc ggcggccgac gcgggtgaag 541 gggcgcagat agtgctgcga ctggcgggtg gcgaggtaga ccacgttggt gccgtcgagc 601 cgggcgaggt ggatggtctc ggtggtgtcg tccgagagcc ggtccagcgt gggccgggcc 661 gccgccacga cctcgtcgcc gtcgatgtag gacgtgccga cgagcagcgc ccgcaccccg 721 atgccgtacc gggtgccggt cgcgtccgtc tccacccagc ccagttcgac cagggtgcgc 781 agcagcatgt acaggctcga cttggggtaa cctacggcct cctgcacgga ggccaggctg 841 tgcatcccgg gccggccggc gaagaactcc agcagttcga ccgtgcggac cgccgacttg 901 acctgggccc cgcccgtctc ggcagctgtc atcgccttga cccctctgtt cgaccagcca 961 tagagtcccg atggaattca tcaaccggga cagcgttcag catatcgaac acccgtcgat 1021 gtgcgagacg caagcagcgg taacacagca ggaggaatcc gcggtgtcag caccagtgtg 1081 gagcgtcgac ccccgaaccg gaaagcagcg cgagcaggta gcggtcgaag ccacagcgga 1141 ggaggtcgac aaggtggtgc gggcggccga cgccgcgcgc ggcgccctta ccgaccgtac 1201 ggtgcgcgcc gccttcctgc gcaccgccgc cgatctgctc gacgaggccc gtgacacgct 1261 cgtcgaggcc gccgacgccg agaccgcgct cggcccggcc cggctcaccg gcgagctggc 1321 ccgcaccagc taccagctcc ggtccttcgc gacgatcgtg gacgagggtg ccttcctcga 1381 cgtccacgtc gaccacgccg acgccaccca gaccccgccg tggcccgatc tgcgccgcta 1441 caagctgccg ctcggcgtcg tcgccgtcta cgccgccagc aacttcccgc tggccttctc 1501 cgtgcccggt ggcgacaccg ccagcgccct cgccgcgggc tgcccggtcg tcgtcaaggc 1561 ccaccccgac caccccgcga cctccgaggt ggccgcctcc gcgctgcgcc gggccgccga 1621 gcaggtcggg ctgcccgccg acgtgatctc cgtggtgcac ggcttcgacg cgggcctcga 1681 actcatcaag cacccgctga tcgccgccgc gggcttcacc ggctcgatcc gcggcggccg 1741 cgccctgtac gacgccgcgg ccgcccggcc ggtgccgatc cccttccacg gcgaactggg 1801 cagcctcaac ccggtcgtgg tcaccgaggc cgccgccgcc gagcgcggcg agcagatcgg 1861 ctccgggctc agcggctcga tgacgctggg cgtgggccag ttctgcacca agcccggctt 1921 cgtgctggcc cccgcgggcg ccgctgggga cgcgctggtc aagtcgctca ccgaggccgt 1981 cagcaacacc gacgccgggg tgctgctcga ccaccgcatg cgcgacaact tcgtggcggg 2041 cgtcaaggag cgtgccgagc tggcggacgt cgaggcgccg gtgacccccg gttccggtgg 2101 cgagcacagc gtcagccccg gcttcctcac cgtcccggcc cggcgcctcg ccgagcaggg 2161 cccgcacgat gtgctgctgg aggagtgctt cggcccggtg accgtcgtcg cccggtacga 2221 gagcgacgcg gagatctccg ccgtcctcgg ccgcctccag ggcaacctga ccgccaccgt 2281 ccagatctcc gccgccgagg ccgagggcac cgagggccgg gccggtgagc tgatcgccga 2341 gctgaccccg ctcgcgggcc gggtcctggt caacggctgg ccgaccggcg tcgccgtcgc 2401 ccccgcccag caccacggcg gcccgtaccc ggcgacgacg tccacctcca cctcggtggg 2461 cggcaccgcc gtcgagcgct ggctgcggcc cgtcagctac caggacaccc cgcccgcact 2521 gctcccgccg gagctgcgcg acgacaaccc gctcgggctg ccccgccggg tgaacggcat 2581 ccgggaacag cagggctgac tccccggtga cgacacggcc gtcgggcgcg cggacctgac 2641 ccgcccgccc ggcggccgtt tcgcgttccg gcgttccgca ttccggtagc gggaatcagt 2701 gcaggtcaga ggggatacgt aagcatccgt ggcgggcacg cttcagagat gtgcgatgga 2761 cgccggggcg ggcccgtccc atagtggatg gacatggaga gcctggcggg cgccgagttc 2821 gcgccacaga cgacgtatct caacaccgct tccagcggtc tgatccccgc acgctccacc 2881 gccgccatga aggcggccct cgacgacgcg gccgtcggcg tccgctatac cgatgcggcc 2941 ttcgaggcgg tcgaggagac ccgcgccgcc tacgcccggc tggtcggcgt accggcacgg 3001 cgggtcgcga cgggcggctc ggtcgtcgtg tacgccggac tgatcgccac ctctctgccg 3061 tccggcgccg aagtcctcac cgccgagggc gacttcagct ccctggtcaa ccccttcgcc 3121 gtacgccgcg acctcaagct gcgcaccgta ccgctggagg agctggcgga ggcggtacgt 3181 ccggaaaccg cgctgatcgc ggtgagcgcc gtccagtcgg cggacggacg gatcgccgac 3241 ctcgacgcga tccgggacgc cgcccgggcg cacggggccc gcaccctggt ggatctcagc 3301 caggccgtcg gctggctgcc ggtgagcgcg ggggaggacg acttcaccgt ggccgtcggc 3361 tacaaatggc tgctgtgccc gcacggcacc gccttcctca ccgtgcccga ggacctgggc 3421 ggcctcaccc cggtcttcgc gggctggcgc tcgggggagc agccgtggga cagctgctac 3481 gggccggtgg ccgagccggc ccgttcggcg cggcgctacg acgagagccc ggccctgctc 3541 gcgtacgtcg cggcccggca ctcgctcgcc ctgctccatg agctgggcac cgaggccgtc 3601 cgcgcccacg accgcgcgct cgccgaccgc ttccgcgccg ggatcaccgg cctggggcgc 3661 tcctgcgtgc ccgcccccgg ctcggtgatc gtctccgtac cgggcctcgg tgaggccgcg 3721 ccccggctgg ccaaggcgga cgtacaggtc tcggcgcggg ccgggaacct gcgcgccgcc 3781 ttccacctct acaacacccc cgccgacgtg gaccgtctgc tggaagtgct gggggacggt 3841 gcgtagcggg aaaacacaga tggccagggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3961 nnnnnnnnnn gccgtcatga tcgcctcgac actggccacc ggcgttctga caccacgctt 4021 cggcccgaaa ccgatcgtcc cgggcgtgta ccgccctccc cctggccacc cacggtcacg 4081 ggaccggtgg ttcacctcac cggggtgttc acctcaccgg ggtgaagtcc cgggccccga 4141 tgtgctccgg ccgccgcacc ggcgccgcga acggctccac cgccgtgttc tccacgctgt 4201 tgaacacgat gaagacgttg ctgcgcgagt agggcgtgat gttgtcaccc gagccgtgca 4261 tgcagttgca gtcgaaccag gtcgccgaac cggccttgcc ggtgaacagc ttgatgccgt 4321 gcttgtcggc gaagccggag agcgcctcgt ccgacggaat cccggcgtcc tgcatctgga 4381 gcgacttctt gtagttgtcc ttcggggtct ggcccgcgca gccgaggaag gtcttgtgcg 4441 aacccggcat gatcatcagg ccgccgttgg tgtcgaagtt ctcggtcagg gcgaccgaga 4501 ccgacacggt ccgcatgttc ggcagaccgt cctcggcgtg ccaggtctcg aagtccgagt 4561 gccagtagaa gcccgaggcc ccgaaacccg gcttgacgtt gatccggctc tggtggacgt 4621 agacgtccga gccgaggatc tgccgggcgc gacccactac ccgcgggtcg cgcaccaggt 4681 tcgcgaacac ctcgctgatc cggtgcacct cgaacaccga ccgtacgtcc tgcgacttcg 4741 gctcgatgat cgagcgctcg tcggcgcgca cgtccgggtc gacgatcagc cggtccagct 4801 cggcgcggta gaccgcgacc tcgtccggag tgatgagctg ctcgatcgcc agaaagccgt 4861 cgcgatcgaa atcgctcagc tcggacggtt gcatcggacc ctcggccccg gggtcggacc 4921 acacgacggg gtccacgcgg ggcgtggcca cctcgagggg accgcgggtc gggtacaggt 4981 cagtgaccgt ggtcatcgga tcatgcctcc tcggtctcgg tgatcagggg gtataccccg 5041 ttctcatcat ggtcctcccg tccggtgatc ggagggttga agacgcaaac gcatcgaaag 5101 tcctttttga tgcgcatggt gtgcttctcg tgcccgtcca gcaggtacat ggtgccgggc 5161 gtgatgatgt gcttctcacc gttctcgtcg ttggtgagct cggcctcgcc ctcgacgcag 5221 aggaccgcct cgatgtggtt ggcgtaccac atggacgtct cggtgcccgc gtacagcgtg 5281 gtctcgtgaa gcgagaagcc gaccttctcc ttcgcgagca cgatgcgctt gctctcccac 5341 gtacccgact tggccttgac gtgcctttcg gttccctcga tgtccttgaa ggatcggacg 5401 atcacggtga gtcgctacct ttctgcagtt acgttactga gggtgttcag gcggtctctc 5461 ggaccgcccg ggcgaggatc cgcagaccct cgtccagctc ttcgggcgtc atcgtcagcg 5521 ccggcagcag cttgacgacc tcgctctccg gaccggaggt ctcgaccagc aggccgagct 5581 cgaaggcgcg cttgcagacg gccgaggcgc gcggcttgtc ctcgaactcc atgccccaca 5641 ccaggccgcg gccgcggtgg ctgatgccgg agttctcgtc gcagatcgcg cgcagcccct 5701 gctcgacctg ctcaccgcgg gccagggtct gcttctccat ctggccgtcg gcccagtagg 5761 tgtccagggt cgcggcggcg gtcacgaacg cggggttgtt gccacggaac gtgccgttgt 5821 gctcaccagg ctcccagatg tccagctccg gcttgaagag ggtgagcgcc agcggcatgc 5881 cgtaaccgct gatcgacttg gagaccgtga cgatgtccgg ggtgatgccc gcctcctcga 5941 aggagaagaa ggcaccggtg cggccgcagc ccatctggat gtcgtcgacg atcagcagca 6001 tgtcctggcg ctcgcacagc tcggacaggg cgcgcagcca ctcggcacgg gcgacgttga 6061 tgccgccctc gccctgcacc gtttcgacga tcacggcggc gggcttgttg aggccggagc 6121 cctggtcctc gagcagccgc tcgaaccaca ggaagtcctc gaccgcgccg tcgaagtagt 6181 tgtcgaacgg catcggggtg ccgtggacca gcgggatacc cgcaccggcc cgcttgaagg 6241 cgttgccggt gacggcgagc gagccaagcg acatgccgtg gaaggcgttg gtgaacgaga 6301 cgatcgactc gcgccccttg accttacggg cgagcttcag cgcggcctcg accgcgttgg 6361 tgcccgtcgg gccggggaac atgaccttgt agggcaggtc acgcgggcgc aggatgttgt 6421 tctggaacga ctccaggaac gcgcgcttgg ccgtggtgga catatcgaga ccgtgcgtga 6481 cgccgtcacg ctcgatgtag tcgatcagcg cccgtttcag gaccgggttg ttgtggccgt 6541 agttgagcga cccggctccg gcgaagaagt cgaggtacgt gtggccgtcc tcgtcgtaca 6601 tgtggctgcc ctgcgcgcgg tcgaagacgg caggccaacc gcggcagtag ctgcgcacct 6661 ccgactccag ggcctcgaag acgctcaggg cgggcggggt gatggtcaca gcattctcct 6721 gggagatgag tgcgaggggt gagggggtga gggcgtgcgc cctcggggtg ttcaggggct 6781 cgctgtacgg gggatcgggg gcagaaatcg gggatgacct cgggatgccg ttaccggggc 6841 tcagccgcgg tccgcgagcg gaccgatgcg gtagagcacc tcgggctcgt gccctccgtc 6901 ggggaacagg ccgccgtcga agagcacctc gtgctcgacg ggcgcgccgt gccgctcggc 6961 gaaggacgtg aacagccggt tcgacgcggt gttgtccggg gtgatcgtgg tctcgacgcc 7021 ccggatgccc gtcgcggcga cgcgcgcggt cagcccgtcc agcagggtgg cggccagtcc 7081 gcgaccgcgc cacgcctgat cgacggcgac ctgccagacc accagggtct cgggccggtc 7141 ggggcggatg tagccggtga tgaagccgac cggtccgccc tcggcgtcgc gcgccaccac 7201 ggaggtggcg gcgaagtcgc ggcaccacaa caggtagctg taggaggagt tgaggtccag 7261 agtcttggag tcgcgggcga ttcgccagat cgcggctccg tcctcgacgc gtggtgtgtc 7321 gagggacagc ccctctggca aatcaatttc gctacgggcg cctactcggt ctgcttgtgc 7381 ggcggtcatg cgaattcaat ttacccagca gaatctaaaa atgcatcgag gagaggggtt 7441 acgtagacgg gggtcgatgt ggtaacacgc gagtgcgcgc gcacgcgcgg cagatcgttg 7501 aaaagcgcta ttttgtgggg gagttagcgg gtcgaaacgg tcggcgtgtg gagtgtgtca 7561 catgtgcgta acgggtgcgt gatgacaccg aattaccgtg tcctggacca ctaaaaaaag 7621 tgcgtttaga gatcggaaga gcgggaagac gagataggaa gatgtgcatg aaaagcgccc 7681 cgaatttgtc ttccggtctt ccggaattca ttccaccggg cttggcgcat cgcttctcgc 7741 acggaacata cccccgaact cacggaaagt cgccacggac ggcgaatgcg ccggagggcc 7801 cgttgccgga ggcggagtag cggtcccgcc cggcgtcccg gctgccctcg gcgtggttgt 7861 actgccccgc gaagacatgg cgcccggccg gcaccgtggc ccccttccgg agcgtccagc 7921 ggtagaccag caccccgccc tcggagcgca ccgagacggc caggtcgtcc acgggcaggg 7981 tgctccagga ccccgtggtg tgcaccccgc cgttctcggc gacccgcagc tccaccgtca 8041 gcgacgtcag cggcttgctc gtggtgaccg tgatgtcact ctgcgcccag tacgggttgg 8101 aattggggtt gatcgccccc cgcgaacgca ccgcggggtc ggcgagatgg tttggaccga 8161 ccgaggtggg ataggcgctc ggcgtgtgcc gaccagcccc gtcccccgcg ccgccgcccg 8221 gccgtcgcgc cgtgccgccg ccctcgcccg cgggcggccc ggccggttcg ctcgacgtcc 8281 gtaccgtctg cggtccgtcg gcggtgtcgg tggtcaccac cgcgatcccg gcggccgcga 8341 ccgccgcggc cagccccgcg gccgggccca cgacccgggt ccactgccgg gcggggaccc 8401 ggcggccgct ccgccgctcg cccgccgcca tgcccgcggt gacccgggcc agcatccggg 8461 cccggtccgg ctggtgcgcc tcggcggcct cgcgcagctc ggcggcgaca tcgcgccggt 8521 cgtcgcgatg gtcgatcatc aaccgttccc tcctgggtgc ccggcatgac ccacacggcc 8581 ggggcggagc ccccgtaccg tctcctgcgc cgacggcgcc ggcggcgggg cctgcgcctg 8641 ggtgcccgcc gcgggccgct cggtgtcgtc gtcgggcggg ccgagcagcc gctccagctc 8701 ggcgatgccg cgggaggtct ggctcttgac cgtgcccacc gagatgccga gcgtacgggc 8761 cgtgtcccgc tccgagaggt cgaacgcgtg ccgcagcacc acgcacgccc gcttccggaa 8821 cggcagcctc accagcgcgg cctgcacatc caccatcgcg gacacatccg gatcgtccac 8881 ccggtccaag cgctccgacg agaagagcgc cacccgccgc cgctcgcgca ccaggctgcg 8941 gatcctggac cgggccaggt tggcgaccac gccccgggcg tacgcggccg gatgctcggc 9001 gacacgcacc cggtcccacc ggtgccagac cgcgaccagc gcgtcggccg ccaggtcgtc 9061 cgccccgtcg gaactgccca gcagcaagtg ggcgaggcgg gccagttcgg catggtgccg 9121 ctcgaagaac tcccggaact ccgcctgtgc gtcggccgcg ggtaagccgt ccgccggtaa 9181 gccggacgtc ggtgtggtgg ccgcggcgga cgggccatcg gtaggagcgc tcacacgggc 9241 ctcctcactg tccctgctgt ccctcgtccg gtgggttgcg tgagcgtagc agcgctcccc 9301 gtgttgtacg agtggagccc ttccgcttcc ccggaattgg ccggtcagcg gccgcccgcc 9361 gtctcgtaca gtgcgtgaca tgagggagag cgcggtgctg cacatcaagg ggcggatcct 9421 ggtcggcccg gaggacgggg acgccggtgg cgtccgggac gagttgtggg tggtcgacgg 9481 gaagatcacc ttcgaccggc cgaccggggc ccgggatgtc cggcagctgg acggctgggt 9541 gctgccgggg ctcgtcgacg cccattgcca cgtcggcctg gacgcccacg gcgcggtgga 9601 cgacgcgacg agcgagaagc aggcactgac cgaacgcgac gccggggcgc tgctgctgcg 9661 cgacgccgga tcaccgtccg acacccgctg ggtcgacgac cgcgaggacc tgccccgcat 9721 catccgggcc ggtcgccaca tcgcccgcac caagcgctac atccgcaact acccgcacga 9781 gatcgaaccg gaggacctgg tcgcctacgt ggcggccgag gcgcgccgcg gcgacggctg 9841 ggtcaagctc gtcggcgact ggatcgaccg ggacagcggc gacctgtccg cctgctggcc 9901 gcgcgggtcg tacgaggcgg ccatcgccga ggcgcaccgg ctcggggcgc gggtcacggc 9961 gcactgcttc gccgaggaga ccctggctcc gctggtcgag gccgggatcg actgcatcga 10021 gcacgcgacc gggctgaccg aggacaccat cccgctgttc gccgagcggg gcgtcgccat 10081 cgtcccgacc ctggtcaaca tcgccacctt cccgcggctc gcggagggcg gcgaggccaa 10141 gttcccgctc tgggccgacc acatgcgacg gctgcacgcg cggcgctacg acacggtgcg 10201 cgccgcctgg gacgcgggca tccccgtcta caccggcacc gacgcgggcg gttcgctcgc 10261 ccacgggctg gtcggccagg aggtcgccga gctggtcaag gcgggcatcc cggtgcggga 10321 cgcgctgtcg gcggccacct ggggcgcccg gacctggctg ggacggccgg ggatcaccga 10381 gggcgccccc gccgacctcg tggtg //