LOCUS       NZ_GG657754            11304 bp    DNA     linear   CON 01-MAY-2024
DEFINITION  Streptomyces himastatinicus ATCC 53653 supercont1.1, whole genome
            shotgun sequence.
ACCESSION   NZ_GG657754
VERSION     NZ_GG657754.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces himastatinicus ATCC 53653
  ORGANISM  Streptomyces himastatinicus ATCC 53653
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
REFERENCE   1  (bases 1 to 10901646)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Annotation of Streptomyces hygroscopicus strain ATCC 53653
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10901646)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q.,
            Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z.,
            Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A.,
            Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L.,
            Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S.,
            Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 10901646)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q.,
            Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z.,
            Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A.,
            Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L.,
            Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S.,
            Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Arachne v. April 2007
            Genome Coverage                   :: 6x
            Sequencing Technology             :: ABI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000158915.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 03:02:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,680
            CDSs (total)                      :: 9,608
            Genes (coding)                    :: 8,720
            CDSs (with protein)               :: 8,720
            Genes (RNA)                       :: 72
            rRNAs                             :: 4, 1 (5S, 16S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 888
            CDSs (without protein)            :: 888
            Pseudo Genes (ambiguous residues) :: 0 of 888
            Pseudo Genes (frameshifted)       :: 423 of 888
            Pseudo Genes (incomplete)         :: 530 of 888
            Pseudo Genes (internal stop)      :: 36 of 888
            Pseudo Genes (multiple problems)  :: 97 of 888
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:01:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2891464
            Orig. end                         :: 2902768
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GG657754.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation was added to the scaffolds in August 2010.
FEATURES             Location/Qualifiers
     region          1..11304
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11304
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11304
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6304](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6304
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(73..86)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.88"
                     /tool="antismash"
     misc_feature    315..328
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.04"
                     /tool="antismash"
     gene            1275..2525
                     /locus_tag="SSOG_RS11630"
                     /old_locus_tag="SSOG_02306"
     CDS             1275..2525
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175673.1"
                     /locus_tag="SSOG_RS11630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_02306"
                     /product="hypothetical protein"
                     /protein_id="WP_167542574.1"
                     /transl_table=11
                     /translation="MAAPLGALTAVAVVVALLIALTSPGDTSGAVDGSVQAAAPVPRAD
                     KVPRGTKKKNKYADWDGKVKVIGDGSTSYTGPQPKQQKIEKLKPGQKPPQFVVFSWDGA
                     LENGDHLFSRFRQVAKESDAQMTFFLSGMYLLPKYKSSLYRPPMHKQGAAAISFPTDGH
                     IRETLEQLRGAWLDGNEIGSHFNGHFCGSKGGGEWTTRQWKSEAEQAYSFVKNWKTNTG
                     FTDIEPLPFDYDEELVGGRAPCLEGQKNLIPAIKSMGWRYDASSPGDFQIWPSKIDGIW
                     NFPLQLVPYPESEKQVLSMDFNFLFNQSGDSTEGDPAKYAEWERLTREGYLNGFERVYN
                     GSRAPLFIGNHFETWNGGIYMQAIEDVMRSVCKRQGVRCVSFKQLADWLDAQDPKVLEK
                     LRMLDPAESPDWKSYTK"
     gene            complement(2604..4658)
                     /locus_tag="SSOG_RS11635"
                     /old_locus_tag="SSOG_02307"
     CDS             complement(2604..4658)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980489.1"
                     /locus_tag="SSOG_RS11635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_02307"
                     /product="transglycosylase domain-containing protein"
                     /protein_id="WP_245606278.1"
                     /transl_table=11
                     /translation="MLGSNNAMGKHRRNAHRKPQPSSAGRGMQLRRWAARHIRIDYPRA
                     GRRGARRWLPSWRQMGLLFVFCLGSLTGVLSFLYMQTEIPKNLNDFATQQDNVYYWADG
                     SEMARTGPVNRQEVALSKVPEKVQWAILAAENASFYSDSGVSPSGLMRAVTRMVTGGET
                     QGGSTITQQYVKNAYLNQRQTFSRKLTEMFMAIKLDDRMTKQEILERYLNTSWFGRGTY
                     GIQRASYAYYGKDVSRLNASEGAMLASLLKGAGTFDPTLSAKNHKRAVERWKWVLDRMV
                     EIGKLSKAERARYTTFPDPHPAPRPAGLTGQVGYLVETAKAYVSSHTEISDADFDLGGY
                     QIHTTFEKSKVKALAASVKKTRDGLDPKNRREDRYVRIGAASVTPGGRIEALYGGPGYL
                     EQGFNDANASVVPAGTAFTPFVYAAALRDGVLKERNSPRTPVAPTTLYDGNNKVAVKTP
                     EGPYWDRGGKIVRGENDGEQSYGQVSLRQAIVQSINTPMMQLGMDVGLDRVRQASIDAG
                     LLPSSFGARVPMFSLGTATPSSIRMASAYATFTANGTHVPPFSVSGLTRNGEKFALRKD
                     RAKRAFSAQVAAQVDDALHGAVQGGTGREAAVVGPDTAGKTGTAQDGTSSWFVGYTDQV
                     STAVSLSRVDPKTQELLPLKGMGGDATGKASDTYPLDIWTRYMAGAGKK"
     misc_feature    4780..4797
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.62"
                     /tool="antismash"
     gene            5001..6304
                     /locus_tag="SSOG_RS11640"
                     /old_locus_tag="SSOG_02308"
                     /pseudo=""
     CDS             5001..6304
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055542.1"
                     /locus_tag="SSOG_RS11640"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_02308"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 9.8e-94, bitscore: 312.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLGTGIGWRPEIAEDIARLPGIDWVEVVAENVCPGHVPDALLR
                     LRERGTTVVPHGVSLGLGGAERPDPERLSALAERAEALGSPLVTEHIAFVRAGGPLTGS
                     PAIEAGHLLPVPRTRDALDVLCENVAIAQAALPVPLALENIAALISWPGEEMTEGQFLA
                     ELVERTGVRLLIDVANLHTNHVNRDEDPAAALDELPTEAIAYVHVAGGVERDGVWHDSH
                     AHPVTQPVLDVLAELCARTTPPGVLLERDEDFPAAEELAGELDAIRAVTTAGVTATDAG
                     PAPKHRPNGNGIPDIDAARERLARAQSALLSALVAGGPVPEGFDRRRLGVQSRALTSKR
                     AGVVAKVAPELPEILGDGYRRAFEGYADGRPMTGATAATRWRSSSICWRPVTSGTRRRG
                     DGSRCGGRSAPDPPRWPRTRWRASRAGPGSC"
     gene            6310..7356
                     /locus_tag="SSOG_RS11645"
                     /old_locus_tag="SSOG_02309"
     CDS             6310..7356
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055541.1"
                     /locus_tag="SSOG_RS11645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_02309"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_106516622.1"
                     /transl_table=11
                     /translation="MNAIVILVYVLVAVASAVIITGVARTRRAAAGAPETGVDDLMEAA
                     FLAGGPGRVADTVISEMHTDGRLAVGRPGVVSIRQPVARTPVENALLDALTSSPNGALD
                     RLRRELMRAPEVQAIGDDLAQRGLLMRPAALRPWRNAARAQVVACWLGVPLGIWLTVRQ
                     NMDGNGGFPVIFLILPALFLGFGVGGICANLAKGRLTSAGRRALDAFRRDTEMGTAERP
                     GGLPSGHWSSAPAVSSANSAARAAPAVAVAVALGGATTIAADTVLRDQLLQAYRVKGAS
                     SGDGSGSSGISDYSGGSWCGGGSGGSGCGGSSCGGGSSCGGGGGGSGCGGGGSGCGGGG
                     GGGCGGGS"
     misc_feature    complement(7361..7374)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.65"
                     /tool="antismash"
     gene            7438..>7789
                     /locus_tag="SSOG_RS11650"
                     /old_locus_tag="SSOG_02310"
     CDS             7438..>7789
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049030.1"
                     /locus_tag="SSOG_RS11650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_02310"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_009714419.1"
                     /transl_table=11
                     /translation="MLWVLFLLVAWGSAVVSCTRLCLAAVAAAQPMEAAAGPRPEGRAL
                     SLYEAAFLAGGPRRVADLALVSMARERRLLLAHTGWVTVVDPDGRDDLERSVIAAIGPR
                     GQSPVPPVRTALA"
     gap             7790..8273
                     /estimated_length=484
     gene            8393..10108
                     /locus_tag="SSOG_RS11655"
                     /old_locus_tag="SSOG_02311"
     CDS             8393..10108
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (137-515). E-value:
                     1.3e-17. Score: 56.2. Matches aSDomain:
                     nrpspksdomains_SSOG_RS11655_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175678.1"
                     /locus_tag="SSOG_RS11655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_02311"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_106516386.1"
                     /sec_met_domain="PF00561 (E-value: 4.8e-22, bitscore: 77.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALLATPATGRPLAAPPTARPTAMAAAGPPAPTAVAAAAPAWSA
                     SFGSTASAVLTSPALAVPRGGAAEARGVIVAAREAGVRGIAFGRCPSSEELSSDVRCGT
                     VSVPLDYARPHGKHIRLTVSRVRASGPKARRQGALVYNPGGPGASGMTFPEYAAEDGFG
                     KTAAVYDFVGYAPRGVGGSAPVSCRRPEAFAKTPTSSPQHPSAAFKRAMVARARAYARG
                     CVRRTGAALRHITTLNNARDLDVLRAALGEKRLTFVGASYGTYYGAVYATLFPRHVRRM
                     VFDSVVNPGPAQIWYRNNLDQSLAFERRWADWRRWTARHHAVYHLGTTPARVLAAYRKA
                     AHRLARKPAGGRVGTGELQDAFVRVGYNDAYWDPSARALAAYLRGNPRPLIALAASESG
                     DAVADENGAAVYTAVECNDAPWPRRWASWNRENTALARRAPFETWDNVAMNLPCAYWPL
                     GHDTPMDVTARPGLPPVLLLAAERDAATPYAGAKELWRRLPGASLVTERKAGTHGLWGG
                     PNACVNRKVDTYLLTGKTPGRTASCAPRPAPEPRPAPVPESGKQPALPGGPGAP"
     aSDomain        8804..9937
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SSOG_RS11655_Abhydrolase_1.1"
                     /evalue="1.30E-17"
                     /label="SSOG_RS11655_Abhydrolase_1.1"
                     /locus_tag="SSOG_RS11655"
                     /protein_end="515"
                     /protein_start="137"
                     /score="56.2"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGMTFPEYAAEDGFGKTAAVYDFVGYAPRGVGGS
                     APVSCRRPEAFAKTPTSSPQHPSAAFKRAMVARARAYARGCVRRTGAALRHITTLNNAR
                     DLDVLRAALGEKRLTFVGASYGTYYGAVYATLFPRHVRRMVFDSVVNPGPAQIWYRNNL
                     DQSLAFERRWADWRRWTARHHAVYHLGTTPARVLAAYRKAAHRLARKPAGGRVGTGELQ
                     DAFVRVGYNDAYWDPSARALAAYLRGNPRPLIALAASESGDAVADENGAAVYTAVECND
                     APWPRRWASWNRENTALARRAPFETWDNVAMNLPCAYWPLGHDTPMDVTARPGLPPVLL
                     LAAERDAATPYAGAKELWRRLPGASLVTERKAGTHGLW"
     gene            complement(10157..10906)
                     /gene="hemQ"
                     /locus_tag="SSOG_RS11660"
                     /old_locus_tag="SSOG_02312"
     CDS             complement(10157..10906)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175679.1"
                     /locus_tag="SSOG_RS11660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_02312"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_009714421.1"
                     /transl_table=11
                     /translation="MTAAPETPDTPDTVTSSKAPNAGKKAKDLNEVIRYTLWSVFRLRD
                     VLPEDRTGYADEVEQLFAQLAAKDVTVRGTYDVSGLRADADVMIWWHSETSDALQEAYN
                     LFRRTRLGRALEPVWSNMALHRPAEFNKSHIPAFLADENARDYVSVYPFVRSYEWYLLP
                     DEERRTMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRASE
                     ARRHVREEVPFYTGRRKPVSELVAGLA"
ORIGIN
        1 gggcgaccgc gatggcctgg acctgggcgc tggcgctcgt cgacggctgg gggcggggcg
       61 tggccgggcg gctgacgcag cgtcatgagt atctgcgcgg catcgaccgc ttccacgaca
      121 tcggcgccgc gctccgcggc ttcaccgggc acatcctgat cacccagccc gaccactggc
      181 ccgcgcacat cgccgggcat ccgccgggcg ccgtgctcac cttcgtcggg ctggaccgcc
      241 tcgggctcgg cggcggcgga tgggcggggg cgttctgcat caccgccgga agctcggcgg
      301 tggccgccgt cctggtcacg ctgcgcgcac tgggccagga gcgcgcggcg cgcaccgccg
      361 cgcccttcct cgtactggcc ccggccgcgg tgtgggtggg ggcgtcggcg gacggctact
      421 tcgcggcggt cgcggcctgg gggctggcac tgctcgcgct ggcggcgacc cgtacgaccc
      481 gggcgccggg cgccgtcgcg ctggccgccg ggctgctgct cggcctcacc gcgtacgtgt
      541 cgtacgggct cacgctcatg gcgctgcccg ccgcggccgt gctgctcctc gcccgtacgg
      601 tgaggcccct gccgtacgcg ctggccgggg cggcggcggt ggcgggcgcg ttcaccctgg
      661 ccggtttcgc ctggtgggag gggtacgacc tgctggtcga gcgctactac caaggggtcg
      721 cggcggaccg cccctacggc tactgggtgt gggccaacct cgccaacgcg gcgatcgcgg
      781 ccgggctcgc ctccgtcgcc ggggcccgcc gggcagtggg ggccgcgccg ggtgcggtac
      841 ggcggctgcg ccgcggggat ctgggcggtg agggcggggc ggacgggctc gtggtgctcg
      901 tgctcgccgc actgctcgcg ctggccaccg ccaatctgtc cgggatgagc aaggccgaga
      961 cggagcgcat ctggctgccg ttcgtgctct ggctgcttcc ggccgccgcc ctgcttcccg
     1021 cacgtgacct gcgccgctgg ctggcggcgc aggcggctct cgcgctgctc gtgaaccatc
     1081 tgctgctcac cggttggtga gtgtccggca gtgagtagcc gatgtgaaga gcaggtgata
     1141 cttctgtgat actgagcggg gcacacaact gaaccgttgc cagggggcgg gacccgttga
     1201 aggcgttgaa gaccttgaag gcatcacagt ccttgaaggc gttgaaggca ttatcggcgc
     1261 ggaacgccta tggcatggcc gcgcccctgg gcgcgctgac ggccgtggcc gtggtcgtgg
     1321 cgctgctcat tgccctgacc tcaccgggcg atacgagcgg cgcggtggac ggcagcgtcc
     1381 aggccgccgc ccccgtgccg cgtgcggaca aggtccccag gggcaccaag aagaagaaca
     1441 agtacgccga ctgggacggc aaggtgaagg tgatcgggga cggctcgacg tcctacaccg
     1501 gcccccagcc gaagcagcag aagatcgaaa agctgaagcc ggggcagaag cccccgcagt
     1561 tcgtggtctt ctcctgggac ggcgcgctgg agaacggcga tcatctcttc tcccgcttcc
     1621 gtcaggtcgc caaggagagc gacgcccaga tgacgttctt cctgagcggc atgtatctgc
     1681 tgcccaagta caagagcagc ctctatcggc cgccgatgca caagcagggc gcggccgcca
     1741 tatccttccc caccgacggg cacatacgcg agacgctgga acagctgcgc ggggcgtggc
     1801 tggacggcaa tgagatcggc tcacacttca acgggcattt ctgcggctcc aagggcggcg
     1861 gtgagtggac cacccggcag tggaagagcg aggccgagca ggcgtactcc ttcgtgaaga
     1921 actggaagac caataccggt ttcaccgaca tcgagccgct gcccttcgac tacgacgagg
     1981 aactggtcgg tggccgcgcc ccctgcctcg aaggacagaa gaatctgatc ccggccatca
     2041 agtcgatggg ctggcgctac gacgcgagtt ctccgggcga cttccagata tggccctcca
     2101 aaatcgacgg catctggaac ttcccgctcc agctggtgcc gtacccggag agcgagaagc
     2161 aggtcctctc catggacttc aatttcctct tcaaccagtc cggtgacagc accgagggcg
     2221 atccggcgaa gtacgccgaa tgggagcggc tgacccgaga gggctatctg aacggtttcg
     2281 agcgcgtcta caacggcagc cgggcaccgc tgttcatcgg caaccacttc gagacctgga
     2341 acggcggcat ctatatgcag gccatcgaag acgtgatgcg cagcgtgtgc aagcgccagg
     2401 gtgtgcggtg cgtatcgttc aagcagctcg ccgactggct ggacgcgcag gatccgaagg
     2461 tcctggaaaa gctgcggatg ctcgacccgg cggaatcgcc ggactggaag tcgtacacca
     2521 agtagcgata cggcgagaag taaaaggccc ggcccggtgt gtcgaaggaa acgcaccggg
     2581 ccgggctttt gtctgtcttc cggttacttc ttcccggccc ccgccatata gcgggtccag
     2641 atgtccagcg gataggtgtc gctcgccttt ccggtggcgt ctccgcccat tcccttgagc
     2701 ggcagcagtt cctgggtctt ggggtccacc cgcgacagcg acacggccgt ggacacctgg
     2761 tcggtgtagc ccacgaacca ggacgaggtg ccgtcctgcg ccgtgcccgt cttaccggcc
     2821 gtatcgggcc cgaccacggc cgcctcccgg ccggtgccgc cctggaccgc gccgtgcagc
     2881 gcgtcgtcca cctgcgccgc cacctgggcg ctgaacgccc gcttggcgcg gtccttgcgc
     2941 agggcgaact tctcgccgtt gcgggtcagt ccgctcaccg agaacggcgg cacatgggtg
     3001 ccgttcgcgg tgaacgtcgc ataggcgctg gccatccgga tggagctggg cgtcgcggtg
     3061 ccgagcgaga acatgggcac gcgcgcgccg aagctgctgg gcagcagccc cgcgtcgatg
     3121 gaggcctggc ggacgcggtc gaggccgacg tccatgccga gctgcatcat cggggtgttg
     3181 atggactgga cgatcgcctg gcgcaggctg acctgcccat aggactgctc gccgtcgttc
     3241 tcgccgcgca cgatcttgcc gccgcggtcc cagtaggggc cctcgggggt cttcacggcg
     3301 accttgttgt tgccgtcgta gagcgtggtg ggggcgacgg gcgtgcgggg gctgttccgc
     3361 tccttgagca cgccgtcgcg cagcgccgcc gcgtagacga agggcgtgaa ggcggtaccg
     3421 gccgggacga cggaggcgtt cgcgtcgttg aagccctgtt ccagatagcc ggggccgccg
     3481 tacagcgcct cgatgcgccc gcccggcgtc acggaggcgg cgccgatgcg tacgtaccgg
     3541 tcctcccgcc ggttcttggg gtcgaggccg tcgcgggtct tcttcaccga ggcggcgagc
     3601 gccttgacct tcgacttctc gaaggtggtg tggatctgat agccgcccag gtcgaagtcg
     3661 gcgtcggaga tttcggtgtg ggaactcaca tacgccttcg cggtctccac cagatagccg
     3721 acctgtccgg tgagcccggc cggccggggc gccggatggg ggtcggggaa cgtggtgtac
     3781 ctcgcccgct cggccttgga gagctttccg atctcgacca tccggtccag cacccatttc
     3841 cagcgctcga ccgcccgttt gtggttcttg gcactgagcg tcgggtcgaa ggttccggcg
     3901 cccttgagca gtgaggcgag catcgcgccc tcgctcgcgt tgagccggga gacgtccttg
     3961 ccgtagtagg cgtaggaggc ccgctgaatg ccgtaggtgc cgcgcccgaa ccagctggtg
     4021 ttcagatagc gttcgagaat ctcctgcttg gtcatccggt cgtcgagctt gatggccatg
     4081 aacatctcgg tgagcttgcg ggagaacgtc tggcgctgat tcagataggc gttcttcaca
     4141 tactgctggg tgatggtcga gccgccctgg gtctcaccgc ccgtcaccat ccgggtcacg
     4201 gcgcgcatca gaccgctcgg ggagacgcca ctgtccgagt agaaacttgc gttctcggcg
     4261 gcgaggatcg cccactggac cttttccggc accttggaca acgccacctc ctgacggttg
     4321 accgggcccg tgcgggccat ctcggagccg tcggcccagt agtagacgtt gtcctgctgg
     4381 gtggcgaagt cgttgaggtt cttgggtatc tcggtctgca tgtagagaaa gctcagtaca
     4441 ccggtcagac tcccgaggca gaacacgaag aggagaccca tctgacgcca cgacggcagc
     4501 caccgccgcg cgccccgtct cccggcccgg gggtagtcga tccggatatg gcgggccgcc
     4561 cagcgacgca gctgcatccc gcgcccggcc gacgacggct gcggcttacg gtgtgcgtta
     4621 cggcggtgct tccccatggc attgttgctg cccagcacgt gtgttccccc acgctcgctc
     4681 cgcgcgcggg cgcggcacaa aggtgtgatc ttccatcccc ccagaccaca cgaccttatt
     4741 ctcccttccg aacatctgtc gccacagggc cccaaaattt tgtaacagtg ctgatattcc
     4801 gcaactcacc gccccgccac ggccgtcgga gaccgtgaag gcgggcgacc cggagggccc
     4861 gcggaggggc gtagcgtgac accccgaagc tcaaatgaac ctctgaacca caagcgccgt
     4921 ggattcgtcc tcccctccag gggtcattac cccgacaggt gagaacgccc ggcatgagac
     4981 cggcaggaga agggggagcc atgacgcgtc tcggtaccgg aatcggctgg cgaccggaaa
     5041 tcgccgagga catcgcgcgc ctgcccggca tcgactgggt tgaggtggtg gccgagaacg
     5101 tctgccccgg ccatgtgccg gacgcgctgc tgcggctgcg cgagcgcggt acgacggtgg
     5161 tcccgcacgg cgtctcgctc ggcctcggcg gcgccgagcg gccggatccc gagcggctga
     5221 gcgccctcgc ggagcgggcg gaggcgctgg gctcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtacgggc cggggggccg ctgaccgggt cgcccgccat cgaggccggg catctgctgc
     5341 cggtgccgcg cacccgggac gcgctggacg tgctgtgcga gaacgtggcc atagcccagg
     5401 cggcgctgcc ggtgccgctc gcgctggaga acatcgcggc gctgatctcc tggcccggcg
     5461 aggagatgac cgaggggcag ttcctggccg agctggtcga gcggaccggt gtgcggctgc
     5521 tcatcgacgt cgccaatctg cacaccaacc acgtcaaccg ggacgaggac ccggccgcgg
     5581 cgctggacga gctgccgacc gaggccatcg cgtacgtcca tgtggcgggc ggggtcgaac
     5641 gggacggggt gtggcacgac agccacgccc acccggtgac gcagccggtg ctcgacgtgc
     5701 tcgccgagct gtgcgcccgt acgacgccgc ccggggtgct gctggagcgg gacgaggact
     5761 tcccggcggc cgaggagctg gcgggcgagc tggacgcgat ccgcgcggtg acaacggccg
     5821 gggtgacggc caccgatgcc gggcccgcgc cgaagcaccg tccgaacgga aacggcatac
     5881 cggacatcga cgccgcacgg gagcggctgg cgcgcgccca gagcgcgctg ctgtcggcgc
     5941 tcgtcgcggg cggcccggtg ccggagggct tcgaccggcg gcggctgggg gtccagagcc
     6001 gggcgctgac gtccaagcgg gccggggtgg tcgccaaggt cgcccccgag ctgccggaga
     6061 tcctgggcga cggctaccgg cgggcgttcg aggggtacgc cgacggccgc ccgatgaccg
     6121 gcgctaccgc cgcgacgcga tggcgttcgt cgagcatctg ctggcggccg gtgacatcgg
     6181 ggacgcggcg acgcggcgac ggctcacgct gtggtggcag gagcgctccg gacccgcccc
     6241 gctggcctcg cacccgctgg cgcgcttcgc gcgccgggcc cggctcgtgc tgagcggggg
     6301 gtgacgggca tgaacgcgat cgtgatcctc gtgtacgtgc tggtggccgt ggcgtcggcc
     6361 gtgatcatca cgggcgtcgc acggacccgg cgggccgccg cgggagcgcc ggaaaccggc
     6421 gtcgacgacc tcatggaggc cgcgttcctg gcgggcgggc ccggccgggt ggccgacacc
     6481 gtgatcagcg agatgcacac cgacggccgg ctggcggtgg gcaggcccgg ggtggtctcg
     6541 atacggcagc cggtcgcccg caccccggtc gagaacgcgc ttctcgacgc tctcaccagc
     6601 tccccgaacg gcgccctcga ccggctgcgc cgggagctga tgcgcgcccc ggaggtgcag
     6661 gccatcgggg acgacctggc acagcgcggg ctgctgatgc gccccgccgc cctccgcccg
     6721 tggcggaacg cggcgcgggc gcaggtggtg gcctgctggc tgggcgtgcc cctgggcatc
     6781 tggctgaccg tccggcagaa catggacggg aacggcgggt tcccggtgat cttcctgatc
     6841 cttccggcgc tgttcctcgg cttcggggtc ggcggcatct gcgcgaacct ggccaagggc
     6901 cggctgacgt cggccgggag gcgggccctg gacgccttcc ggagggacac cgagatgggc
     6961 accgccgagc ggcccggagg gctgcccagc gggcactggt cgtcggcgcc cgccgtatcc
     7021 tccgcgaact ccgcggcgag ggcggcgccc gccgtcgcgg tcgccgtggc gctgggcggc
     7081 gcgaccacca tcgccgccga cacggtgctg cgggaccaac tgctccaggc gtatcgggtg
     7141 aagggcgcgt cctcgggcga cggttcgggc tcgtcgggca tctccgacta ctccggcggc
     7201 tcgtggtgcg gcgggggctc cgggggctcc ggctgcggcg gctcgtcgtg cggaggcggc
     7261 tcctcatgcg ggggcggggg cggcggctcc ggctgcggcg ggggcggttc gggctgcggc
     7321 ggcgggggcg gtggcggctg cggcggaggc agctgaccgc tcacgaatcg tggcaccgaa
     7381 tcgtgcatat cgcgaaatct gtggcgcaga agcggtcggg tccagtagaa ctcggccatg
     7441 ttgtgggtcc tgttcctgct cgtcgcatgg ggctcggcgg tcgtcagctg cacccgcctg
     7501 tgcctcgccg cggtcgcggc cgcccagccg atggaagccg ccgctggtcc gcggcccgag
     7561 gggcgggccc tgagcctgta tgaggcggcg ttcctggcgg gcgggccgcg gcgggtcgcc
     7621 gatctcgccc tggtgtcgat ggcccgtgag cgccggctgc tgctcgccca caccggctgg
     7681 gtcaccgtcg tggacccgga cggccgggac gatctcgaac gctcggtcat cgccgcgata
     7741 gggccgcggg gccagtcccc cgtaccgccg gtccgcaccg cgctggccgn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctttaca
     8281 tgacccagaa ccgcaccaaa tatccctttt attgcgtgat gtgcggaggg atggcttgtg
     8341 agagcagcag cgctgtacgg catcctcggg accgctgtcc ctgtccgtgg ccatgacggc
     8401 gctgctcgcc acccccgcca ccggcagacc gctcgccgcg ccaccgaccg cacgccccac
     8461 cgccatggcc gccgccgggc ccccggcgcc gaccgccgtc gcggcggcgg cgcccgcctg
     8521 gtccgcctcg ttcggctcca ccgcctccgc cgtgctgacc tcccccgcgc tggccgtgcc
     8581 ccggggcggc gccgccgagg cccgcggggt gatcgtggcg gcccgggagg cgggcgtccg
     8641 gggcatcgcc ttcggccgct gcccgagctc cgaggagctg tcgtccgatg tccgctgcgg
     8701 caccgtctcc gtaccgctgg actacgcgcg gccgcacggc aagcacatcc gtctcaccgt
     8761 cagccgcgtc cgcgcctccg ggcccaaggc ccggcggcag ggcgcgctgg tctacaaccc
     8821 gggcggaccg ggcgcgtccg gcatgacgtt ccccgagtac gccgccgagg acggcttcgg
     8881 caagacggcc gcggtctacg acttcgtcgg ctacgcgccc cgcggggtcg gcggctccgc
     8941 accggtgtcc tgccggcgcc cggaagcctt cgccaagacg ccgaccagct ccccgcagca
     9001 cccctccgcc gcgttcaagc gggcgatggt ggcgcgggcg cgcgcgtacg cccggggctg
     9061 tgtgcgccgt acgggggcgg ccctgcggca catcaccacg ctgaacaacg cccgcgacct
     9121 cgatgtgctg cgcgccgcgc tcggcgagaa gcggctcacc ttcgtgggtg cctcgtacgg
     9181 cacgtactac ggcgccgtct acgcgacgct cttcccccgc catgtgcgcc gcatggtgtt
     9241 cgacagcgtg gtcaaccccg gccccgcgca gatctggtac cgcaacaacc tggaccagtc
     9301 cctggccttc gagcggcgct gggcggactg gcgccgctgg accgcccggc accacgccgt
     9361 ctaccacctc ggcacgaccc ccgcccgggt actcgccgcg taccggaagg cggcccaccg
     9421 cctggcgcgg aaaccggcgg gcggccgcgt cggcaccggg gagctccagg acgcgttcgt
     9481 acgggtgggc tacaacgacg cctactggga ccccagcgca cgggccctcg ccgcctatct
     9541 gcggggcaat ccccggccgc tgatcgcgct ggccgcgtcc gaatccgggg acgcggtggc
     9601 cgacgagaac ggcgccgccg tctacaccgc cgtggagtgc aacgacgcgc cctggccgcg
     9661 ccgctgggcg agctggaacc gggagaacac cgccctcgcg cgccgcgcgc ccttcgagac
     9721 ctgggacaac gtggcgatga acctgccctg cgcctactgg ccgctcgggc acgacacgcc
     9781 catggacgtg accgcccggc cggggctgcc gccggtgctg ctgctggcgg ccgaacggga
     9841 cgcggccact ccgtacgcgg gcgccaagga gctgtggcgc agactccccg gcgcctccct
     9901 ggtgaccgag cggaaggccg gtacccacgg cctgtggggc ggcccgaacg cctgcgtcaa
     9961 ccgcaaggtc gatacgtatc tgctgaccgg gaagacgccg ggccggaccg cctcctgcgc
    10021 accgcgcccg gcaccggagc ctcgcccggc cccggtgccc gagtccggca agcaacccgc
    10081 gctccccgga ggcccggggg cgccgtgacg gtcagggccg tgacctcagg gcctcagggc
    10141 ctcagggccg tgaccgtcag gccagaccgg cgaccagttc ggagaccggc ttgcgccgcc
    10201 cggtgtagaa cggcacctcc tcgcgcacat gacggcgcgc ctcggaggcg cgcagatgac
    10261 gcatcaggtc gacgatccgg tgcagctcgt ccgcctcgaa ggccaggatc cactcgtagt
    10321 cgccgaggga gaacgacgcg acggtgttgg cgcgcacatc ggggaagccg cgcgccatct
    10381 tgccgtggtc cgcgagcatc gtgcggcgct cctcgtcggg cagcagatac cactcgtagg
    10441 accgtacgaa ggggtagacg ctgacgtagt cgcgcgcgtt ctcgtccgcg aggaaggcgg
    10501 ggatgtgcga cttgttgaac tccgccgggc ggtgcagcgc catgttcgac cacaccggct
    10561 ccagggcgcg gccgagccgg gtgcggcgga agaggttgta cgcctcctga agggcgtcgg
    10621 aggtctccga gtgccaccag atcatgacgt ccgcgtccgc ccgcagcccc gagacgtcat
    10681 aggtgccgcg cacggtgacg tccttggcgg cgagctgcgc gaagagctgc tcgacctcgt
    10741 cggcgtagcc ggtgcggtcc tccggcagca cgtcgcgcag ccggaacacc gaccacaggg
    10801 tgtagcggat gacctcgttg aggtccttgg ccttcttccc cgcgttgggc gccttggacg
    10861 acgtgacggt gtcgggggtg tcgggggtct cgggagcagc agtcatggtg cctattctcg
    10921 ctctccgccg acggcgcccg ccgccagggt cgccgtcagc agctcgtcgg cggccgtgcg
    10981 ggcggatccg atgcacgcgg ggatgccgac gccgtcgtag accgcgccgc acacccgcag
    11041 cccgggcagc ttggcgacct ccgcgcggat ccgggcgacc cgctcgagat ggccgaccgg
    11101 gtactgcggc agcccgccgt cccagcggct cacccgggtc gccaccggcc gggcgtcgag
    11161 tccggtggcc gcgccgagat cggcgagcga catccccacc agatcggcgt cgtcgcgctt
    11221 cagatgctcc tcgtcctcga accgcccgat cgaggtacgc agcacgaaga gatcgggggc
    11281 ggcgtcgcgg agccagcccc attt
//
