LOCUS NZ_GG657754 32122 bp DNA linear CON 01-MAY-2024 DEFINITION Streptomyces himastatinicus ATCC 53653 supercont1.1, whole genome shotgun sequence. ACCESSION NZ_GG657754 VERSION NZ_GG657754.1 KEYWORDS WGS; RefSeq. SOURCE Streptomyces himastatinicus ATCC 53653 ORGANISM Streptomyces himastatinicus ATCC 53653 Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group. REFERENCE 1 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D., Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Annotation of Streptomyces hygroscopicus strain ATCC 53653 JOURNAL Unpublished REFERENCE 2 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Direct Submission JOURNAL Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA REFERENCE 3 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Direct Submission JOURNAL Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: Arachne v. April 2007 Genome Coverage :: 6x Sequencing Technology :: ABI ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_000158915.1-RS_2024_05_01 Annotation Date :: 05/01/2024 03:02:38 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 9,680 CDSs (total) :: 9,608 Genes (coding) :: 8,720 CDSs (with protein) :: 8,720 Genes (RNA) :: 72 rRNAs :: 4, 1 (5S, 16S) complete rRNAs :: 4, 1 (5S, 16S) tRNAs :: 64 ncRNAs :: 3 Pseudo Genes (total) :: 888 CDSs (without protein) :: 888 Pseudo Genes (ambiguous residues) :: 0 of 888 Pseudo Genes (frameshifted) :: 423 of 888 Pseudo Genes (incomplete) :: 530 of 888 Pseudo Genes (internal stop) :: 36 of 888 Pseudo Genes (multiple problems) :: 97 of 888 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 20:01:42 NOTE :: This is a single region extracted from a larger record! Orig. start :: 1037600 Orig. end :: 1069722 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to GG657754.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation was added to the scaffolds in August 2010. FEATURES Location/Qualifiers region 1..32122 /candidate_cluster_numbers="1" /candidate_cluster_numbers="2" /contig_edge="False" /product="pyrrolidine" /product="butyrolactone" /region_number="7" /rules="(Glycos_transf_1 and Alpha-amylase and Aminotran_3 and DXP_synthase_N)" /rules="AfsA" /tool="antismash" cand_cluster 1..32122 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(Glycos_transf_1 and Alpha-amylase and Aminotran_3 and DXP_synthase_N)" /detection_rules="AfsA" /kind="neighbouring" /product="pyrrolidine" /product="butyrolactone" /protoclusters="1" /protoclusters="2" /tool="antismash" protocluster 1..32122 /aStool="rule-based-clusters" /category="other" /contig_edge="False" /core_location="[10000:22122](+)" /cutoff="10000" /detection_rule="(Glycos_transf_1 and Alpha-amylase and Aminotran_3 and DXP_synthase_N)" /neighbourhood="10000" /product="pyrrolidine" /protocluster_number="1" /tool="antismash" proto_core 10001..22122 /aStool="rule-based-clusters" /tool="antismash" /cutoff="10000" /detection_rule="(Glycos_transf_1 and Alpha-amylase and Aminotran_3 and DXP_synthase_N)" /neighbourhood="10000" /product="pyrrolidine" /protocluster_number="1" misc_feature 526..544 /note="TFBS match to LexA_variant_3, Repressor of DNA damage response, confidence: weak, score: 20.9" /tool="antismash" misc_feature complement(685..703) /note="TFBS match to LexA_variant_3, Repressor of DNA damage response, confidence: weak, score: 19.31" /tool="antismash" gene 847..1512 /locus_tag="SSOG_RS03790" /old_locus_tag="SSOG_00772" CDS 847..1512 /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1057: TetR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018555250.1" /locus_tag="SSOG_RS03790" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00772" /product="TetR family transcriptional regulator" /protein_id="WP_009712882.1" /transl_table=11 /translation="METGTSQREDHQQEAAARRRKELLEAADRVVLRDGPEASMNAIAA EAGITKPILYRHFGDKGGLYRALAVRHTDALLASLRNALDAPAPDRRGRVAATLDTYLA AIEARPQVYRFLMHPADQGQQQEQEQGFDVGHHSAPLLRRMGDELAKVIADRVELGPGG EEVARIWGHGIVGMMHAAGDWWLRERPCSRERLVHHLTDLLWGRLALVDDRIGAPGLF" gene complement(1531..2079) /gene="def" /locus_tag="SSOG_RS03795" /old_locus_tag="SSOG_00773" CDS complement(1531..2079) /EC_number="3.5.1.88" /GO_function="GO:0042586 - peptide deformylase activity [Evidence IEA]" /GO_process="GO:0036211 - protein modification process [Evidence IEA]" /codon_start=1 /gene="def" /gene_functions="biosynthetic-additional (smcogs) SMCOG1275: peptide deformylase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_010988521.1" /locus_tag="SSOG_RS03795" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00773" /product="peptide deformylase" /protein_id="WP_208867323.1" /transl_table=11 /translation="MRNGAIPGSRGTVRPLRLLGDQALRAPCEEVGDFDAALARLVEDL FATMYAAGGVGLAANQIGVGLRVFVYDCPDDEDRRHLGHVVNPRLAEADGVTVRGTEGC LSLPGIESGTPRFDRAVVEGVTATGEPVRVEGSGFFARCLQHECDHLDGGLFLDRLDGL RRRRVLWAARRAPWARGRA" gene 2256..3494 /locus_tag="SSOG_RS03800" /old_locus_tag="SSOG_00774" CDS 2256..3494 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_010062348.1" /locus_tag="SSOG_RS03800" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00774" /product="MurT ligase domain-containing protein" /protein_id="WP_009712884.1" /transl_table=11 /translation="MAGNSEPLSPRAKLAVTAGKAAAAVSKAAGRGSGSVIGGKVALRL DPDLLSRLAHHLDVILVSATNGKTTTTRLIAEALRAAGPVVSNALGANMPAGITSALAG GSDARYGVIEVDEKYLAGVARDTEPKAIALLNLSRDQLDRAAETRMLAEKWREGLAGTK ATVIANADDPLIVWAASSSPNVVWVAAGQEWKDDAWSCPACGGVLQRPGDDWVCGECGF RRPVPSWALNGEYVLDPHGAAWPIHLQLPGRANKANAATSAAVAATFGVPPQVALERMY QVQAVAGRYDVVTFQERELRLLLAKNPAGWLETFSLIDPPPTPVILSVNARGADGTDTS WLWDVDYTRLAGHPICVIGDRKLDLAVRLEVAGLDFRVCADADEAVRISPPGRIEVIAN YTAFQDLRRRVGN" gene 3518..4258 /locus_tag="SSOG_RS03805" /old_locus_tag="SSOG_00775" CDS 3518..4258 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056725.1" /locus_tag="SSOG_RS03805" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00775" /product="glutamine amidotransferase" /protein_id="WP_009712885.1" /transl_table=11 /translation="MRSTSQNGLRLVWVYPDLLSTYGDQGNVLVVERRARQRGLDVSRL DVRSDQQIPTSGDIYLIGGGEDRPQRLAAERLMRDGGLNRAVANGAIVFSVCAGYQIIG HEFVNDLGQRQQGLGLLDVISTRGEGARCVGDVLADIDEPLGLPPLTGFENHQGVTHVG PTARPFARVRFGNGNGTGDGYEGAYKDTVFGTYMHGPVMARNPHIADHLLKLALDVNGL PPVDDQWYEALRNERIAAATAQPA" gene 4428..5453 /locus_tag="SSOG_RS03810" /old_locus_tag="SSOG_00776" CDS 4428..5453 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004934584.1" /locus_tag="SSOG_RS03810" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00776" /product="6-phosphofructokinase" /protein_id="WP_009712886.1" /transl_table=11 /translation="MRIGVLTSGGDCPGLNAVIRSVVHRAVVDHGDEVIGFQDGWKGLL ECDYRKLDLDAVSGILALGGTVLGSTRVQPAHLRDGVERAKGHVEELGLDAVIPIGGEG TLKAARMLSDAGLPIVGVPKTIDNDIASTDVTFGFDTAVGVATEALDRLKTTAESHQRV LIVEVMGRHTGWIALHSGMAAGAHAIVVPERPFDIEELVRTVGARFEAGKKFAIVVAAE GAKPREGTMEYAAGERDVYGHERFAGIARQLSVELEQRLGKEARAVILGHVQRGGTPTA YDRVLATRFGWHAVEAAHRGQFGKITALRGTDIVMVPLADAVERLKTVPAERYTEAECV L" gene 5513..6463 /locus_tag="SSOG_RS03815" /old_locus_tag="SSOG_00777" CDS 5513..6463 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056723.1" /locus_tag="SSOG_RS03815" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00777" /product="cytochrome c oxidase assembly protein" /protein_id="WP_009712887.1" /transl_table=11 /translation="MDHGGHGMMMDLPPFTLSRGLAFGGDPFFLVGCVLALALYGWAAV RLWRRGDRWPVGRLVAFVAGVLTVVVAMCTGLNDYGMVMFSVHMVQHMIISMVSPILLL LGAPVTLALRALPAAGRGRKGPRELLVALLHSRYMRIITHPVFTIPLFIASLYGLYFTP LFDFLMGSKVGHIAMMVHFLAVGLVFFWPIMGVDPGPHRPGYVMRMLELFAGMPFHAFF GIALMMASAPMIGTFEHPPGSLGIDALSDQNAAGGIAWAFSEVPSVIVLVALVYQWYKS DQRQARRDDRTADRDGDQELAAYNAYLASLQARGR" gene complement(6522..8570) /locus_tag="SSOG_RS03820" /old_locus_tag="SSOG_00778" CDS complement(6522..8570) /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1041: transcriptional regulator, SARP family" /gene_kind="regulatory" /inference="COORDINATES: protein motif:HMM:NF015649.4" /locus_tag="SSOG_RS03820" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00778" /product="BTAD domain-containing putative transcriptional regulator" /protein_id="WP_050799756.1" /transl_table=11 /translation="MFFQVLGPVQMLMQHGAALPLRGARLSTLLALLLVQAPAVVKRDF LIEELWAGRPVKDPVNALHLHIAKLRVLLGRNSGLLQTRKGLGYRMGLSADMLDATRFA RLAAEGNDHLDQGRPADAAAVLQEALRLWRGPALEGAGDSVAIAGERARLHELRLVASE HLAEARLAQGQHVALVPELKALVAEHPLRESLRGLLMRALYGAGHQAEALEVFDATRGV LRDALGVSPSPPLRALHAALLAHDTHALNVPRRGPAATTPPPGPRNAPSRVVPGSGSRT TGEAGRTGGLVPPLDAFVGRRADLGAITEAVRQNRLVTVVGPGGVGKTRIALQTADNLR EEFPDGICLVELASLPAGAGPDRLIAAFAEALGIPDSPDRIPQLARLKAALAGKRMLLV VDTCEHVRQGAAHTVDTLMRGCSALTVLATSREALAVPGETAFPLRPLPLGEAVELFRA RMTAATPDLGWDDVTRASAAAVCRRLDCLPLAIELAVGRTRMLTVPDIEARIADGLHIL AQAPVPAPERHRNLYATLDWSHELLGADEKRVFHDLSVLVGTWDLDAAEHFCADTTLSG AEVVDTVGRLVGKSLLAPLRGAGSSRFRMLETVRVYAQQKLAASGREEEVRNRLAAWTS TQPESAARSGPKLHGGRPPARPIAPALRDAMWTARSQGMCRSCSLTS" misc_feature complement(7845..7847) /note="tta leucine codon, possible target for bldA regulation" /tool="antismash" misc_feature complement(8421..8423) /note="tta leucine codon, possible target for bldA regulation" /tool="antismash" misc_feature 8651..8666 /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: weak, score: 16.42" /tool="antismash" gene 8942..9964 /locus_tag="SSOG_RS03825" /old_locus_tag="SSOG_00779" CDS 8942..9964 /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) APH" /gene_kind="biosynthetic-additional" /inference="COORDINATES: protein motif:HMM:NF013775.4" /locus_tag="SSOG_RS03825" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00779" /product="phosphotransferase" /protein_id="WP_009712889.1" /sec_met_domain="APH (E-value: 5.7e-18, bitscore: 64.8, seeds: 156, tool: rule-based-clusters)" /transl_table=11 /translation="MSPCRPLSAGGTAPLSHTAGLWYVERPEGGYVLKVQLNPDAAREP RFYPLKEEVLAHCRSHGVPAPAAVAASDGATSVWHDGLVCELAPLAPGAASGELTPAQA AAVVRTALGLRRALDRPPDGVADRLAPIRLPRLVDEEHWPSALRDADERLLPLAERRGD SWGRAAASALRGVIASGRLLREAGPAAAGEQPARPAVVHGDLHYHHFLFSPTGDGEGEG DGPDVVAVLDFDNLHVGDRLLDLAWLAEMAGRVAGETSRRRSLAAFREAATAAGLLAPG EERLLMPLLMAHSLPVIVDIAKDILLRDILSPAWTEYFDLLAPPRRLRIHQSLVSPLPR " gene 10001..11320 /locus_tag="SSOG_RS03830" /old_locus_tag="SSOG_00780" CDS 10001..11320 /GO_function="GO:0008483 - transaminase activity [Evidence IEA]" /GO_function="GO:0030170 - pyridoxal phosphate binding [Evidence IEA]" /NRPS_PKS="Domain: Aminotran_3 (31-376). E-value: 3.5e-77. Score: 251.6. Matches aSDomain: nrpspksdomains_SSOG_RS03830_Aminotran_3.1" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) pyrrolidine: Aminotran_3" /gene_functions="biosynthetic-additional (smcogs) SMCOG1013: aminotransferase class-III" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016576029.1" /locus_tag="SSOG_RS03830" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00780" /product="aminotransferase class III-fold pyridoxal phosphate-dependent enzyme" /protein_id="WP_009712890.1" /sec_met_domain="Aminotran_3 (E-value: 7.4e-77, bitscore: 257.8, seeds: 13, tool: rule-based-clusters)" /transl_table=11 /translation="MSVQESTLSSWPTDLSAYCFEVPHSEDAVLFTSGKGIRLQDAGGR SYLDGLSGVFVTCFGYDCEPIKRAVEDQLRTLPFNPPLHGTNHAALELARGLVDLAPPG ITAAKLVNGGSESVEAAVRLARLYHRTHGNPDKIKVLSHYHGYHGATFGSLSLTGRPDV GRFGPGLGGFLHVWPPDCLESFWGVPPEESGALAASIIERTIEAEGPDSVAALVIEPVI HLRGMAIPSADYLTRLREICDRHNVLLVFDEIVTGFGRTGKPFAAQTFGVTPDIMCVGK GISGGYAPLAAVLMAERIAAILGDSGGPLAFAPSHTYAANPMASAAGLAAVNLFRDGDF PVRIGKLSEYIEPRLRELIGGRGTVRSIGLLYGVKLLEDGGERPIGERFARACLQRGLI IRGQDDWFVMAPAFVTTETEADEMLRVLAAALDEVCGSGR" aSDomain 10094..11128 /aSDomain="Aminotran_3" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_SSOG_RS03830_Aminotran_3.1" /evalue="3.50E-77" /label="SSOG_RS03830_Aminotran_3.1" /locus_tag="SSOG_RS03830" /protein_end="376" /protein_start="31" /score="251.6" /tool="antismash" /translation="TSGKGIRLQDAGGRSYLDGLSGVFVTCFGYDCEPIKRAVEDQLRT LPFNPPLHGTNHAALELARGLVDLAPPGITAAKLVNGGSESVEAAVRLARLYHRTHGNP DKIKVLSHYHGYHGATFGSLSLTGRPDVGRFGPGLGGFLHVWPPDCLESFWGVPPEESG ALAASIIERTIEAEGPDSVAALVIEPVIHLRGMAIPSADYLTRLREICDRHNVLLVFDE IVTGFGRTGKPFAAQTFGVTPDIMCVGKGISGGYAPLAAVLMAERIAAILGDSGGPLAF APSHTYAANPMASAAGLAAVNLFRDGDFPVRIGKLSEYIEPRLRELIGGRGTVRSIGLL YGVKL" gene 11317..13131 /locus_tag="SSOG_RS03835" /old_locus_tag="SSOG_00781" CDS 11317..13131 /GO_function="GO:0008661 - 1-deoxy-D-xylulose-5-phosphate synthase activity [Evidence IEA]" /GO_process="GO:0016114 - terpenoid biosynthetic process [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) pyrrolidine: DXP_synthase_N" /gene_functions="biosynthetic-additional (smcogs) SMCOG1204: transketolase" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016576030.1" /locus_tag="SSOG_RS03835" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00781" /product="1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein" /protein_id="WP_009712891.1" /sec_met_domain="DXP_synthase_N (E-value: 1.9e-06, bitscore: 26.4, seeds: 174, tool: rule-based-clusters)" /transl_table=11 /translation="MTGLSSLFAEPGEAALPRLTPEENKVVRRELAELEAGDYSGPLLD SLRKLAAAVPERVHDDSARVLLRMHEIAGSGNYQSCLSSLPLVRACFELGLVSVDSEEG QGPAELIVGRGHIAPSFYAEHYVRGTFPFTPLATLHQGGLTGVIHRDLGFRNTMRYSLG VGVAQAVSLAGAMAERGDNRKVVCLAGDGELQEGVVFECLRFAHDAGLTNLVLVVDAND MGIEPLPKPLSRDYLAGYLHRVEEVDGLDAKAVSDSLGELLAAPGSAALVCRTRKGDHS FKPKPAEGSAAPAKPAKVSFAATTGRMLADRQERTGRELAVFTADMANRFGLRGKVPYT NTGLAETLSLGLTLALPERTLKVVATDAMYYMDSLSMLTEATTSVRNLLVLAGRSWGAW GGAHNAANLLGLVMNTRVYEPVTEAEFDACLDRLDHNPDITHVLSTVDAKFDPPGADCS ADVDGGVWITPPAAGDLGTALVTFGYATVLVAEANRDLGLPHLHCAALDPDLDAETVAR LRGSERLLAIEYNGAHGGFGERLRTKYLLPAEVHGVQRDIANRVHDKQLRLHRMSPAQL RELLDGRPSDTDGEDRPGEEARRAAARA" gene 13112..14491 /locus_tag="SSOG_RS03840" /old_locus_tag="SSOG_00782" CDS 13112..14491 /EC_number="2.4.-.-" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) pyrrolidine: Glycos_transf_1" /gene_functions="biosynthetic-additional (smcogs) SMCOG1045: glycosyl transferase group 1" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016576031.1" /locus_tag="SSOG_RS03840" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00782" /product="glycosyltransferase" /protein_id="WP_009712892.1" /sec_met_domain="Glycos_transf_1 (E-value: 8.5e-13, bitscore: 47.4, seeds: 63, tool: rule-based-clusters)" /transl_table=11 /translation="MRLHAHDYRAPDTSGIVDGAILMHREVAELLAGHRSVAWHDLTRG AESVASASPGEVVYAGAGPYAFLHHLWRERTKGRFRIVREVHTALWSGYWAQEELCAPL LRPGDLVLFPTEYTRRLFIRHLASVTEDNSAVAYPMLDRLPRLPRARIPAPDEPLRIGY LGALSLAKNFDQVLSVFARCHRASPGRVSLLCAGKPNHPRWETDAVLAELGRQGVEPDA VRMLGVLGQTELAGFFSQIDVLLFPSTASRETLGRVVLEALAHGIPVLGADIGPAVELL PARNLVPTVLNTGTAFSMSRVEALGWVDEDALVDKLRARDFEPADVIAESPYQDRTFLR ALSGEAVRQEIAHDARLTDRLTVERRKGVDLDRALARSEQIFTDFFRHRDDGLLSAVDA LPEEPAQGRRQLRRLITEGSRNLADYRAFPRLLDALLLPPLDYGLTPEPVRTTHRKDMT A" gene 14488..15345 /locus_tag="SSOG_RS03845" /old_locus_tag="SSOG_00783" CDS 14488..15345 /NRPS_PKS="Domain: PKS_KR (21-190). E-value: 1e-16. Score: 53.3. Matches aSDomain: nrpspksdomains_SSOG_RS03845_PKS_KR.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) adh_short" /gene_functions="biosynthetic-additional (smcogs) SMCOG1001: short-chain dehydrogenase/reductase SDR" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016576032.1" /locus_tag="SSOG_RS03845" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00783" /product="SDR family NAD(P)-dependent oxidoreductase" /protein_id="WP_050799757.1" /sec_met_domain="adh_short (E-value: 1.7e-32, bitscore: 112.0, seeds: 230, tool: rule-based-clusters)" /transl_table=11 /translation="MTTTERGTGTDTGTGTGLAGKVALVIGGGRGIGAAAVEALARQDA TVVVADTDRLPSAFNHYRSTRVSGFADAQKLAARLSEAGLRVTAAEVDATDEAAVRELY GSLAGGPGRLDVVVNAFGVTHVSPVEQMELTEFQQVVSGNLDGVFLSSKHAVPLLRDSG GGSIINFSSVSGRSGFAKVAHYCAAKFGVIGFSSSLALEVARDGIRVNAICPGMIRTNM WSYLLSAFVRPGETEDECWERMRTMIPQREFQTPEDIAELVVFLATAKKVTGQAISVDG GMTA" aSDomain 14551..15057 /aSDomain="PKS_KR" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_SSOG_RS03845_PKS_KR.1" /evalue="1.00E-16" /label="SSOG_RS03845_PKS_KR.1" /locus_tag="SSOG_RS03845" /protein_end="190" /protein_start="21" /score="53.3" /specificity="KR activity: inactive" /specificity="KR stereochemistry: C2" /tool="antismash" /translation="VALVIGGGRGIGAAAVEALARQDATVVVADTDRLPSAFNHYRSTR VSGFADAQKLAARLSEAGLRVTAAEVDATDEAAVRELYGSLAGGPGRLDVVVNAFGVTH VSPVEQMELTEFQQVVSGNLDGVFLSSKHAVPLLRDSGGGSIINFSSVSGRSGFAKVAH YCAAKF" CDS_motif 14557..14631 /aSTool="nrps_pks_domains" /database="abmotifs" /detection="hmmscan" /domain_id="nrpspksmotif_SSOG_RS03845_0001" /evalue="1.30E-03" /label="PKSI-KR_m1" /locus_tag="SSOG_RS03845" /protein_end="48" /protein_start="23" /score="11.5" /tool="antismash" /translation="LVIGGGRGIGAAAVEALARQDATVV" CDS_motif 14989..15051 /aSTool="nrps_pks_domains" /database="abmotifs" /detection="hmmscan" /domain_id="nrpspksmotif_SSOG_RS03845_0002" /evalue="1.70E-04" /label="PKSI-KR_m4" /locus_tag="SSOG_RS03845" /protein_end="188" /protein_start="167" /score="14.1" /tool="antismash" /translation="INFSSVSGRSGFAKVAHYCAA" gene 15342..15956 /locus_tag="SSOG_RS03850" /old_locus_tag="SSOG_00784" CDS 15342..15956 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016576033.1" /locus_tag="SSOG_RS03850" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00784" /product="DUF1349 domain-containing protein" /protein_id="WP_009712894.1" /transl_table=11 /translation="MTATQPTGAAQAEVVFGFDEGWSWSAEPLSCSRTGGSLEITAPGG ADLFAMPGAHEASGLPLLQRTVRGDFTAWTRISVDGAAFGDAGGIALHGPEGWFKVCVE RVKASGWAVVTVLSRPVSDEALGPALAGPEAELLVTREGPRQAVFFREAPEDDWRFVRT FFGFSEPELRLGLFVQAPFTPSASAVFAPVRLRPTALRDRR" gene 15959..17206 /locus_tag="SSOG_RS03855" /old_locus_tag="SSOG_00785" CDS 15959..17206 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016576034.1" /locus_tag="SSOG_RS03855" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00785" /product="MFS transporter" /protein_id="WP_154685886.1" /transl_table=11 /translation="MPVPPAAPARVRVVAPLVLSLLLGVVTAALGAALPLLRSAYGLPE GSGAELVGLYNLGALAGVAACGLGGRTPAGGSTTGRPVLPLLAAFAGGCAGMALAPTWW LLNAFATATGFGFGGLLLYLNAFFGRFPGRRGLLLMNLLHACFGAGATVGPLLVGRFGE VPWLLLVTAGLTAPLLWPTRHLGTAGPGAPPTPDTGTDAPAGRRSLPLLAVFAAVAFLY AGVEAGTGALESTHLEAAGRSAESAAGLTALFWAGLTAGRVVLPPVTHRLGPPRLVLLC LLAATGLLLTTLDDRWAPAGYGLAGFALGTVFPTVLVWATAALPLPPERVSAVLLLTNL IGSAALPPLLGLFAAPARAAVSIPLSIATLTLLCGLAVTAAVALARRADPTAARRHPDR PDRPDRPDRPDQKVRT" gene 17203..18198 /locus_tag="SSOG_RS03860" /old_locus_tag="SSOG_00786" CDS 17203..18198 /GO_function="GO:0008171 - O-methyltransferase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (smcogs) SMCOG1042: O-methyltransferase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016576035.1" /locus_tag="SSOG_RS03860" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00786" /product="methyltransferase" /protein_id="WP_009712896.1" /transl_table=11 /translation="MTSPQPVTELFWAYWKTKTMLSAIELGVFTELAKEPLRGDELAGR LGLGPGAVGDFLDALVALDLLDRTGDVYANTPAADEYLDAGKPATYLGDSYLTQCAGLA DDLTGVLRTGEALRGARTGRDFYADTYATSNSVRAFQRDMTALSIGSARVIADSFPWDD YRSLADIGTSEGALPGRILLRHPHLTAVGFDLPQAREGFEEYTGALDLADRMSFQPGDF FRDELPSADVLVFGHILHNWGLEEKRLLLDKAYRALPEGGAVLIYETLIDGDRRSNAVG LILSLMMHLEVPGGFDYTGPECQEWLREAGFRETRVEHLDGPESMIIGFK" cand_cluster 18039..28815 /candidate_cluster_number="2" /contig_edge="False" /detection_rules="AfsA" /kind="single" /product="butyrolactone" /protoclusters="2" /tool="antismash" protocluster 18039..28815 /aStool="rule-based-clusters" /category="other" /contig_edge="False" /core_location="[23038:23815](+)" /cutoff="20000" /detection_rule="AfsA" /neighbourhood="5000" /product="butyrolactone" /protocluster_number="2" /tool="antismash" proto_core 23039..23815 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="AfsA" /neighbourhood="5000" /product="butyrolactone" /protocluster_number="2" gene 18246..19067 /locus_tag="SSOG_RS03865" /old_locus_tag="SSOG_00787" CDS 18246..19067 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003954424.1" /locus_tag="SSOG_RS03865" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00787" /product="endonuclease/exonuclease/phosphatase family protein" /protein_id="WP_039940608.1" /transl_table=11 /translation="MFWNLYEAGIDRLEGRDERWLLQRDIVAEHRPRVLMTTEGWGWDR DGGRFFEDAKAGFGMDGALFTAKTGCHLAIFWQRDIEPVETERTPHDLAAWHGHGSATL RLPGWSHELRFVVAHLDPFSPTNRRIESDRLRRYANPGAPAPTVMAMDANSLPPDDPEP DWTTVPRHRRDDHRWPGEKHADRAPLRRLLGEAEDPLFVDAGAHARDRSPTFGHHPPGQ APRRIDVFLLSPALTGALASYQAVDDPRLLPHDGRPGASDHRPITLRLRRR" gene 19092..19700 /locus_tag="SSOG_RS03870" /old_locus_tag="SSOG_00788" CDS 19092..19700 /EC_number="2.3.1.-" /GO_function="GO:0016407 - acetyltransferase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Mac" /gene_functions="biosynthetic-additional (smcogs) SMCOG1156: Chloramphenicol acetyltransferase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003954426.1" /locus_tag="SSOG_RS03870" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00788" /product="sugar O-acetyltransferase" /protein_id="WP_009712898.1" /sec_met_domain="Mac (E-value: 5e-11, bitscore: 41.8, seeds: 568, tool: rule-based-clusters)" /transl_table=11 /translation="MDDDREATDERGHLDALKARMAGGLPYVSTEPALMPYRERCARLL DAFNATALTDDEARQPILHELLGRVGPGGWVMPRFLCEFGFFIELGAEIRINFDAVLLD CAPIVLGSRVWLAPRCQLYTADHDLDPGRRSAMWERARPITIGDDVWLGGGVIVLPGVT IGERTVVGAGSVVTKDLPADVIAAGNPARVIREVPRPQP" gene 19739..20434 /locus_tag="SSOG_RS03875" /old_locus_tag="SSOG_00789" CDS 19739..20434 /GO_function="GO:0016787 - hydrolase activity [Evidence IEA]" /GO_function="GO:0046872 - metal ion binding [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) HAD_2" /gene_functions="biosynthetic-additional (smcogs) SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016578440.1" /locus_tag="SSOG_RS03875" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00789" /product="HAD family phosphatase" /protein_id="WP_009712899.1" /sec_met_domain="HAD_2 (E-value: 1.1e-26, bitscore: 93.2, seeds: 50, tool: rule-based-clusters)" /transl_table=11 /translation="MDPAIQAIVFDHDGVLVDSIRPHFLACSALFREHGAELPQGWWAR EVCGSPDAHPLLFGMLRNSAGAATGTDAQLEDRLEALWAEHFTPENVRLMPGVRELLAA LRTAGLPLAVASSADEGWVRRWLRHYELDGSFATVVTGDQVPRRKPDPAVYLETAARLS VAPRRCVVFEDSVSGVAAARAAGMTVLAVPTPLTRACDYSLAHRVLPDLTVVDAAELFL ADPARAGQP" gene 20473..22122 /locus_tag="SSOG_RS03880" /old_locus_tag="SSOG_00790" CDS 20473..22122 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) pyrrolidine: Alpha-amylase" /gene_functions="biosynthetic-additional (smcogs) SMCOG1257: Alpha-glucosidase" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014044294.1" /locus_tag="SSOG_RS03880" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00790" /product="glycoside hydrolase family 13 protein" /protein_id="WP_039936500.1" /sec_met_domain="Alpha-amylase (E-value: 1.9e-59, bitscore: 201.0, seeds: 31, tool: rule-based-clusters)" /transl_table=11 /translation="MPQVPAWWRSAVIYEIYLRSFADGNGDGTGDLAGVRARLPYLARL GVDALWFTPWYPSPMADGGYDVVDYHGVDPLFGTLDEAQRLIAEALELGIRTVVDIVPN HVSSAHPWFRAALAAPPGSAERALFHFRPGRGPSGQEPPNNWRSEFGGPAWTRTAGPDG SPGEWFLHLFSPDQPDLNWGHPRVRDAHEEVLRFWLNRGVAGLRVDSATLLAKDPALPD LADPGLAPEGPSHPYADREELHGLYRSWRRITDSYPGDRALIGEMWLHEPGRFARYLRP DELHTAFNFDFLRSPWDAGQLRRCVDATLAAHGGAPACWVLCNHDVTRTVTRYGRARTG FDFAAKAHGTPTDLALGTRRARAAALLTLALPGSVYLYQGEELGLPEVEDIPADRLRDP MHFRSGGEDPGRDGCRVPLPWSGAAPPYGFTDEDAEPWLPQPKGWAELTVEAQEGAAGS MLELYREALRLRRALPALHGAPLEWLPGGPGVLAFRRGDVVCVVNLSDRAVPLPEGHTV LLTSEPGPPGGAALAPDAAAWTVQDGKDGGHA" gene 22115..23002 /locus_tag="SSOG_RS03885" /old_locus_tag="SSOG_00791" CDS 22115..23002 /codon_start=1 /inference="COORDINATES: protein motif:HMM:NF005112.0" /locus_tag="SSOG_RS03885" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00791" /product="prephenate dehydrogenase" /protein_id="WP_009712901.1" /transl_table=11 /translation="MRSAAVIGTGLIGTSVALALTGHGVTTYLLDRDPEAARTAAALGA GLAATPRAPVDLAVVAVPPAHTASVLKEYQQRMVARFFTDVASVKTRPLMDAASAGCDL TAYVGGHPMAGRELSGPLAARADLFQGRTWVLTPSAFSRAEALHHATELIELCGGRPVI MGHAEHDRAVALTSHAPHMMASLLAGRLLGAETAQLRLVGKGLRDATRIAAGDAALWAD IIGSNAGPVARVLAQLAEELGAVVAALRELDDSAARPGGKGAADLLAALARGEEGRSRI VAAERPADAARAAR" gene 23039..23815 /locus_tag="SSOG_RS03890" /old_locus_tag="SSOG_00792" CDS 23039..23815 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) butyrolactone: AfsA" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018093172.1" /locus_tag="SSOG_RS03890" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00792" /product="AfsA-related hotdog domain-containing protein" /protein_id="WP_106516348.1" /sec_met_domain="AfsA (E-value: 2.2e-15, bitscore: 55.6, seeds: 34, tool: rule-based-clusters)" /transl_table=11 /translation="MNDEIVFIVGDRLAGGPPRAGLMPLSRLIADLRAGRLRDPLPALV PGQGIERHEMDLIRAELKRCRLPGTALWDAVPPELAGRGEVHKHRQENVLIAGLRRIGD THYQAALRLSNTHEMVLDHTTGTHVTGMVVTEAVRQMSLAVAERYLLKPGQPVPRFLIN SLQVSFSRFLLPLDAEIDYHLDEAVWKRPDVLRYHGQCDVLQAGVTTASGSMSLSVHEE GAAERIEETQSDAVVRRLADRHQAGRSPEPSEHRVP" misc_feature 23046..23060 /note="TFBS match to ZuR_variant_1, Zinc-responsive repressor, confidence: weak, score: 17.13" /tool="antismash" misc_feature complement(23048..23059) /note="TFBS match to CcpA, Catabolite control protein A, confidence: weak, score: 16.41" /tool="antismash" gene 23835..24482 /locus_tag="SSOG_RS03895" /old_locus_tag="SSOG_00793" CDS 23835..24482 /EC_number="2.1.1.100" /EC_number="2.1.1.334" /GO_function="GO:0008168 - methyltransferase activity [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003953130.1" /locus_tag="SSOG_RS03895" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00793" /product="isoprenylcysteine carboxylmethyltransferase family protein" /protein_id="WP_009712903.1" /transl_table=11 /translation="MTWLLRRFPLFVLTVSVMATALATVREAAGWSDPAHRVGCALAAS YLLWLLLESWTTVRSSRATEATTDRGTVYAYGAARAVTVATACLVPTDWSGYRPWMPVL PVAFVCAVALRLAAVRALGRFYSHRVRALGDHRIVDTGPYRLVRHPAYVGMAAAHIAFV GYFLNPASAAALVVLLLPAIVVRVLVEERMLFALPGYSDFAASRRRLIPWVW" gene 24490..27234 /locus_tag="SSOG_RS03900" /old_locus_tag="SSOG_00794" CDS 24490..27234 /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_function="GO:0016301 - kinase activity [Evidence IEA]" /GO_process="GO:0016310 - phosphorylation [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) PPDK_N" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003953129.1" /locus_tag="SSOG_RS03900" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00794" /product="PEP/pyruvate-binding domain-containing protein" /protein_id="WP_009712904.1" /sec_met_domain="PPDK_N (E-value: 5.5e-54, bitscore: 182.7, seeds: 105, tool: rule-based-clusters)" /transl_table=11 /translation="MTIENVLRAVRPAGRRGPVLRGRALLDEAEVGPKFARQARMASAG LPVPHFFCLPASLFTRTLAPVRPRLLAILRRLDHTDPDRLAEQSDALRRLVLDVPVPPS VARAVHRAYDEVFGPHAVVSVRSSMVGPRGRAAEDSAEDAFAGIGESFLYVTRDQVLER VRACWASGFGTRALLYRAARSTAATEDFAVCVGVQRMVFGRRSFVLFTCDPLTGSRDRV LAAGLGIGEGVVQEKVPVDHHFIDRRTGAVRSVVAHKSERMEEDPARPGSGPRLLPVHG RERDEPALTDGQIRHIRDVGDRLERLFGCPQDIEGTLTEDGALHILQTRPVVLDLGGQR LWSNANITESYPGVTTALTYSFAQRFYREDFRDFYRRLGVSPAVLARHENDLRSMLGLL NGRVYYSLSAWYTLHRLSPTFPLWRASFERMMGMSPSVTDVRPDRLATSPGRLPETAAA LLRLAWLRALHVPAARSFDRWWQQTIARHREAARTADPLQLARLMRSLWQEAGERWGLT LVNDVLLQTTETAIMRLFSRWLPGADAGLHSDLLCGGEENRSVTILMSLVDLAERIRAT PGLLDALADEDPGKPPEELLRRIEDGAFGTDLADRMRAHAAAHGDRGLQELKLEVPVPR EQPSQLLRLAGEYARGGLTRECLRGRETATRRAAEERLRTLLAGHPVRRRVLRTLLAQQ RRYINIREDTRYSRSELFGFGRQIYRRLGTELAARGALDAAEDVVHLTEEEILGHFDGG AVTDDLRGLARLRRAEYLSRATLDAELPPDFATMGPVRDGLPTPGGDARPGSRLRGLGS SGGVVRGTARVVIDPHQRVDPTDDLILVARETDPGWLFLMLSARGIVVERGTLLSHTAI SGRKFGIPTVVALPGATRRIPDGARVEMDGATGTVTILDEVAE" gene 27231..>27927 /locus_tag="SSOG_RS03905" /old_locus_tag="SSOG_00795" CDS 27231..>27927 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003963309.1" /locus_tag="SSOG_RS03905" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00795" /product="hypothetical protein" /protein_id="WP_009712905.1" /transl_table=11 /translation="MSGTVRRLYAFFAERAPARVYVTYAVLWVLALEGALALVEPGDAR WMLGVPTLAEIGTVWLALLFIRVVDEQKDLGYDREHNPGRPLPRGSITVRELRLAMAVI AAAGIALTVWRSPLLTGWLLLDLAYICFLVGLERWSRSVRDGIFRNLAVSYPVQALLSG HVWLSYRDATGAGPHWSAAVPVLLCACVFLHFEFARKTSHRVTPGATLYSNAIGTRGSV GLTVGCALAA" gap 27928..28107 /estimated_length=180 gene 28201..29223 /locus_tag="SSOG_RS03910" /old_locus_tag="SSOG_00796" CDS 28201..29223 /NRPS_PKS="Domain: PKS_ER (105-338). E-value: 1.3e-17. Score: 55.8. Matches aSDomain: nrpspksdomains_SSOG_RS03910_PKS_ER.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) ADH_zinc_N" /gene_functions="biosynthetic-additional (smcogs) SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019071732.1" /locus_tag="SSOG_RS03910" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00796" /product="NADP-dependent oxidoreductase" /protein_id="WP_009712906.1" /sec_met_domain="ADH_zinc_N (E-value: 4.3e-17, bitscore: 61.5, seeds: 86, tool: rule-based-clusters)" /transl_table=11 /translation="MAAPVPVPSTHREVRLTARPGGGPRPDRFEVAEVPVPVPRHGQVL VRNRLTRVGAVTRTLMEDAAGVLPMSPYAPGRALWAPALGEVVTAPGTRLGPGDLVRHQ LGWREYAVIEADDARRVDPALWPDPAACLSQGFAGWLAVVRAAAVQPGDTVFVTGAAGG AGSIAGQFARLRGAGRVIGSTGSRRKADYLVGTLGFDATVVRGEAPIEEQLRAAAPEGI DVLVDTVGGEQLRAALALARRGARCVVIGALSAQARGGTTAEVPVDTLALFSGGITLRG LTTADHRDAVPRWEEQFSRGLRAGVLTFPHTRLRGIERAPRALGELLDGHHIGTVLVES " aSDomain 28516..29214 /aSDomain="PKS_ER" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_SSOG_RS03910_PKS_ER.1" /evalue="1.30E-17" /label="SSOG_RS03910_PKS_ER.1" /locus_tag="SSOG_RS03910" /protein_end="338" /protein_start="105" /score="55.8" /tool="antismash" /translation="GWREYAVIEADDARRVDPALWPDPAACLSQGFAGWLAVVRAAAVQ PGDTVFVTGAAGGAGSIAGQFARLRGAGRVIGSTGSRRKADYLVGTLGFDATVVRGEAP IEEQLRAAAPEGIDVLVDTVGGEQLRAALALARRGARCVVIGALSAQARGGTTAEVPVD TLALFSGGITLRGLTTADHRDAVPRWEEQFSRGLRAGVLTFPHTRLRGIERAPRALGEL LDGHHIGTVLV" gene 29348..29500 /locus_tag="SSOG_RS53225" /old_locus_tag="SSOG_00797" CDS 29348..29500 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019070924.1" /locus_tag="SSOG_RS53225" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00797" /product="hypothetical protein" /protein_id="WP_039936502.1" /transl_table=11 /translation="MTGSAKGMTACAVGGLVAVTAYTVALGSNGWLWFAWVVLALLTVG TLLTR" gene complement(29513..30850) /locus_tag="SSOG_RS03920" /old_locus_tag="SSOG_00798" CDS complement(29513..30850) /GO_function="GO:0004497 - monooxygenase activity [Evidence IEA]" /GO_function="GO:0005506 - iron ion binding [Evidence IEA]" /GO_function="GO:0016705 - oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen [Evidence IEA]" /GO_function="GO:0020037 - heme binding [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (smcogs) SMCOG1007: cytochrome P450" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056721.1" /locus_tag="SSOG_RS03920" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00798" /product="cytochrome P450" /protein_id="WP_234365217.1" /transl_table=11 /translation="MGLYERLRSEHGPVAPVRLGDDVPAWLLLGYRENLEVARTPSRFS RDSRYWSAWGEGRIPDDSPLLPVVGWQPMCTFADGDEHERLRAALTDSMNRVDRRGIRR HITRFTHQLVNDFCADGQAELVTGFAQRLPMLVLTQLLGMPDEYGPRLVEATRDLMKGS ETALRSDQYVTDTLRQLVERKRTQPGSDLASWLLSHPSELTEEEVVQHLRLVLLTGNET TTNLMANTLRMVLTDPRFRASLAGGHMTLPDAIEHVLWNEPPLTVIPGRWATGDTELGG RQIKAGDMLLLGLAAGNVDPAIRPDLGAPMYGNRSHLSFSGGPHECPGQDIGRAIADTA IDTLLLRLPDLRLDVPETELNWIASWISRHLVALPVAFMPRRAETDPVDTSGMLLAPKP RPTRWVPADEDEALSAGEDATDGTAAPRARVPWWTSLWRWLRGTAA" misc_feature complement(29612..29614) /note="tta leucine codon, possible target for bldA regulation" /tool="antismash" gene complement(30940..31563) /locus_tag="SSOG_RS03925" /old_locus_tag="SSOG_00799" CDS complement(30940..31563) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056720.1" /locus_tag="SSOG_RS03925" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00799" /product="ATP/GTP-binding protein" /protein_id="WP_009712909.1" /transl_table=11 /translation="MDYRTSETLVGPRSEDALPASAAAAVKIVIVGGFGVGKTTMVGSV SEIRPLTTEETMTQAGVGVDDIAGVERKTETTVAMDFGRISLNEQLVLYLFGTPGQERF WFLWNGLFEGALGAVVLIDTRRLEVSFDVLGRLEERGVPFVVAINAFPGAPVHPVDELR AALDLPDAVPIIDCDARDRGSSRDTLLTLVSYLQHLTTVTPETP" gene complement(31544..31903) /locus_tag="SSOG_RS03930" /old_locus_tag="SSOG_00800" CDS complement(31544..31903) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018846603.1" /locus_tag="SSOG_RS03930" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00800" /product="DUF742 domain-containing protein" /protein_id="WP_009712910.1" /transl_table=11 /translation="MSRTGKGTDEGGPERLYLVDGGPGRSQRADTIELVSLIVARAEPE PGMLPEAAAVLRMCHFPLSVAEISAYLTLPVSTVTVLLADLLAHRQVEARAPVPAAVLP DTELLQAVMHGLQNL" ORIGIN 1 ttgtcgagga ccacctcggc ggtgtgcgag gcgcggatgc cgtgcttctt gaacttctgg 61 ccctgggaca gcccgggggt gtccggcggg atgatgaagg acgcgtggcc cttggagccg 121 agtccggggt cgacggccgc gacgacgacg tggacgctcg cgatgccgcc gttggtggcc 181 caggtcttgg tgccgttgat gacccactcg tccttggcct cgtcgtagac ggcccgggtg 241 cgcatggcgg acacgtccga gcccgcgtcg ggctcggagg agcagaacgc ggcgaccttc 301 acgtcattgg cgtcgccgta catctgcggg atccaggtgc cgatctgctc ctcggtgccg 361 ttggcgagca cgccgacggc ggccagaccg gtgccgacga tggacagggc tagccctgcg 421 tcgccccaga acagctcctc catggtcatg gggatgccga ggccggtggg gtcgaagtac 481 tgctgggcgt agaaatccag ggagtacagg ccgatcttgg ccgcctcctg gataatcggc 541 cagggcgttt cctcgcgctc gtcccattcc gcggcggcgg gtcgcatgac gtccgcggcg 601 aagccgtgga tccactcacg cactgccttc tgatcatcgt tgagttcgaa cgagaactcc 661 gccatggccc ctccacctgc cgcgcctgct gttacctacg gtaacagcag tctgttaccg 721 gtcagtaggc gatgtcaact ccacggccgt gcgatctctt taccccggcc gggtgttacg 781 ttgcgcagat acgcggtgcc cattcatgta acgcgtgaac acgtcagggg gcggggaggc 841 cacgacatgg agaccgggac cagccagcgc gaggaccacc agcaggaggc cgccgcacgg 901 cgccgcaagg agctgctgga ggccgccgac cgggtggtgc tgcgcgacgg ccccgaggcc 961 tcgatgaacg cgatcgccgc cgaggcgggc atcaccaagc ccattctcta ccgccacttc 1021 ggcgacaagg gagggctgta ccgtgccctc gcggtgcgcc acaccgatgc cctgctcgcc 1081 tcgctgcgca acgccctgga cgcccccgcg cccgaccgcc gcggacgggt ggcggccacg 1141 ctggacacct acctggcggc gatagaggcc cggccccagg tgtaccgctt cctgatgcac 1201 ccggccgacc agggccagca gcaggaacag gagcagggct tcgacgtggg ccaccactcc 1261 gcgccgctgc tgcgccgcat gggcgacgag ctggccaagg tcatcgccga ccgggtcgaa 1321 ctcggccccg gcggcgagga ggtggcccgg atctggggcc atgggatcgt cggcatgatg 1381 cacgccgcgg gcgactggtg gctccgggaa cggccctgct cgcgcgagcg gttggtccac 1441 cacctcaccg atctgctgtg ggggcggctg gcgctggtgg acgaccgtat cggcgccccc 1501 gggctgttct gaggcccccg gttcacgggt tcacgcccgc ccgcgcgccc acggcgcgcg 1561 gcgggccgcc cacagcaccc ggcgccgccg cagcccgtcc agccggtcca ggaacagccc 1621 gccgtccagg tggtcgcatt cgtgctggag acagcgggcg aagaagccgc tgccctcgac 1681 ccgcaccggc tccccggtcg ccgtcacgcc ctccaccacc gcgcggtcga agcgcggggt 1741 gcccgactcg atgcccggca gcgacagaca gccctcggtg ccccgtacgg tcaccccgtc 1801 cgcctccgcc agccgcgggt tgaccacgtg ccccagatga cgccggtcct cgtcgtccgg 1861 gcagtcgtac acgaagaccc gcagccccac gccgatctgg ttggcggcga ggccgacccc 1921 gcccgccgcg tacatcgtgg cgaacaggtc ctcgaccagc cgggcgaggg ccgcgtcgaa 1981 gtcgccgacc tcctcacagg gcgcgcgcag cgcctgatca ccgagcagcc gcaggggccg 2041 taccgtcccc cggctccccg ggatcgcgcc gtttcgcatg gcagccaagc gtacggtccc 2101 ccggaagggc cgacggaatt cgggacagcg cccggatctc gataggctga tccccgaccg 2161 tggcctcccg gctggcgatt cggcgcggag ggggcgtacc accggtgcct cggggggcag 2221 gtgcggcgcc ggtatgcgag gaggatcgag agacgatggc aggcaactca gagccgctgt 2281 cgccacgtgc caaactggcc gtgacggcgg gcaaggccgc ggcggcggtg tcgaaggccg 2341 cgggccgcgg cagcggatcg gtgatcggcg gcaaggtggc cctgcgcctc gaccccgatc 2401 tgctcagcag gctggcgcac cacctggacg tgatcctggt gtcggcgacc aacggcaaga 2461 ccacgacgac ccggctgatc gccgaggcgc tccgggccgc gggcccggtg gtctccaacg 2521 cgctcggggc caacatgccc gcgggcatca cctcggccct ggcgggcggc tccgacgccc 2581 gctacggcgt gatcgaggtc gacgagaagt acctcgccgg ggtggcacgc gacaccgagc 2641 ccaaggccat cgcgctgctc aacctctcgc gcgaccagct cgaccgggcc gccgagaccc 2701 ggatgctcgc cgagaagtgg cgggagggcc tggccggtac gaaggccaca gtgatcgcga 2761 acgcggacga cccgctcatc gtctgggccg cctcctcaag ccccaatgtg gtgtgggtcg 2821 ccgcgggcca ggagtggaag gacgacgcct ggtcctgccc ggcgtgcggc ggggtgctcc 2881 agcgccccgg cgacgactgg gtgtgcggcg agtgcggctt ccgccgcccg gtgcccagct 2941 gggcgctgaa cggtgaatac gtgctggacc cgcacggcgc ggcctggccg atccacctcc 3001 agctgcccgg ccgcgcgaac aaggcgaacg cggccacctc ggcggccgtc gccgccacct 3061 tcggcgtgcc gccgcaggtc gcgctggagc ggatgtacca ggtgcaggcg gtcgccggcc 3121 gctatgacgt ggtcaccttc caggagcgcg agctgcggct gctgctggcg aagaacccgg 3181 ccggctggct ggagacattc tcgctgatcg acccgccgcc caccccggtg atcctctcgg 3241 tgaacgcgcg cggcgcggac ggcaccgaca cctcctggct gtgggacgtg gactacaccc 3301 ggctcgccgg gcacccgatc tgtgtgatcg gcgaccgcaa gctggacctc gcggtgcgcc 3361 tggaggtcgc cgggctcgac ttccgggtct gcgcggacgc ggacgaggcg gtacggatct 3421 ccccgcccgg ccgcatcgaa gtgatcgcca actacaccgc gttccaggac ctgcgccgcc 3481 gggtcggcaa ctgacccccg tacgaggaga cacgacgatg cgtagcacga gccagaacgg 3541 cctgcgtctg gtctgggtct accccgacct gctgagcacg tacggcgacc agggcaacgt 3601 cctggtggtg gagcgccggg cgcgccagcg cgggctggac gtctcccgcc tggacgtccg 3661 ctccgaccag cagatcccca cctccgggga catctacctg atcggcggcg gcgaggaccg 3721 cccgcagcgg ctggccgccg agcggctgat gcgggacggc gggctgaacc gcgcggtcgc 3781 caacggggcg atcgtcttct cggtgtgcgc cgggtaccag atcatcggcc acgagttcgt 3841 caacgacctc gggcagcgcc agcagggcct gggcctgctg gacgtgatca gcacccgcgg 3901 cgagggcgcc cgctgcgtcg gcgatgtgct ggccgacatc gacgagccgc tggggctgcc 3961 gccgctgacc gggttcgaga accaccaggg cgtcacccac gtcggcccga ccgcgcgccc 4021 cttcgcgcgc gtccggttcg gcaacggcaa cggcacgggc gacggctacg agggcgcgta 4081 caaggacacc gtcttcggga cgtacatgca cggcccggtg atggcccgca acccgcacat 4141 cgcggaccat ctgctgaagc tggcgctcga tgtgaacggg ctgccgccgg tcgacgacca 4201 gtggtacgag gcgctgcgca acgagcggat cgcggcggcc accgcccagc ccgcctgagc 4261 ggcctcgcgc acggctgagc cgccgggtga ccgcatcgcg gacacccggc ggcattgtgc 4321 tttccgcggc gtccgccgct tgtagggtgt cgtcgtccag ccggacagcg cggtccggat 4381 gtcgctccca tacacatggg ccgtagcaga cagaggaatc acgcgccatg cgcatcggtg 4441 tgctcacatc cggcggcgac tgccccgggc tgaacgcggt catcaggtcc gtcgtccacc 4501 gcgccgtcgt cgaccacggc gacgaggtga tcggcttcca ggacggctgg aagggcctgc 4561 tggagtgtga ctaccgcaag ctcgacctgg acgccgtgag cggcatcctg gccctcggcg 4621 gcaccgtcct gggctcgacc cgggtacagc ccgcgcatct gcgcgacggg gtggagcgag 4681 cgaagggcca cgtcgaggag ctgggcctgg acgcggtcat cccgatcggc ggcgagggca 4741 ccctgaaggc ggcccggatg ctgtcggacg ccgggctgcc gatcgtcggc gtcccgaaga 4801 cgatcgacaa cgacatcgcc tccaccgatg tgaccttcgg cttcgacacc gcggtcgggg 4861 tggcgaccga ggccctggac cggctcaaga ccaccgcgga gtcgcatcag cgggtgctga 4921 tcgtcgaggt catggggcgg cacaccggtt ggatcgcgct gcactcgggc atggccgcgg 4981 gcgcccacgc gatcgtcgtg ccggagcgcc cgttcgacat cgaggagctg gtcaggaccg 5041 tcggggcacg gttcgaggcg ggcaagaagt tcgcgatcgt cgtggccgcc gagggcgcca 5101 agccgcgcga gggcacgatg gagtacgcgg ccggggagcg ggacgtctat ggccacgagc 5161 ggttcgccgg tatcgcacgt cagctctccg tcgagctgga acagcggctg gggaaggagg 5221 cccgggcggt gatcctgggc catgtgcagc gcggtggcac gccgacggcg tacgaccggg 5281 tgctggccac ccgcttcggc tggcacgccg tcgaggcggc gcaccgcggc cagttcggca 5341 agatcacggc gctgcgcggt acggacatcg tgatggtgcc gctcgcggac gcggtggagc 5401 ggctgaagac ggtacccgcc gagcggtaca ccgaggccga gtgcgtgctg tgatctgacc 5461 ggttctaggc tggtgcgagc acatttcgtg cgcaccggga ggagagctgc tgatggatca 5521 cggcgggcac ggcatgatga tggatctgcc gccgttcacg ctgtcgcgcg gactggcgtt 5581 cggcggtgat cccttcttcc tcgtcggctg tgtgctcgcg ctcgccctgt acggctgggc 5641 cgcggtgcgg ctgtggcggc gcggtgaccg ctggccggtg gggcggctgg tggcgttcgt 5701 ggcgggtgtg ctgaccgtcg tggtcgcgat gtgcaccggg ctgaacgact acggcatggt 5761 catgttcagc gtgcacatgg tccagcacat gatcatcagc atggtctccc ccatcctcct 5821 gctgctgggc gcgccggtga cgctggccct gcgcgccctg ccggcggccg ggcgcggccg 5881 caaggggccg cgggaactgc tggtggcgct gctgcacagc cgctacatgc ggatcatcac 5941 ccacccggtg ttcacgatcc cgctgttcat cgcgagcctg tacgggctct acttcacccc 6001 gctcttcgac ttcctgatgg gctccaaggt cgggcacatc gcgatgatgg tccacttcct 6061 cgcggtgggc ctggtcttct tctggccgat catgggcgtg gacccggggc cgcaccggcc 6121 gggctatgtg atgcggatgc tggagctgtt cgcggggatg cccttccacg cgttcttcgg 6181 gatcgcgctg atgatggcga gcgcgcccat gatcggcacg ttcgagcatc cgcccggctc 6241 gctggggatc gacgcgctgt ccgaccagaa cgcggcgggc ggcatcgcct gggcgttcag 6301 cgaggtcccg tcggtgatcg tgctggtggc gctggtgtac cagtggtaca agtccgacca 6361 gcgccaggcg cggcgcgatg accgtacagc ggaccgcgac ggtgatcagg aactcgccgc 6421 gtacaacgcc tatctggcgt cgctccaggc ccgcgggcgc tagggggtct cgtcggcgtc 6481 cggcgggtcg ccgcgccgcc gcgcagagcc gctgtgtgtg ctcagctcgt gagggagcag 6541 ctccggcaca tgccctggct cctcgcggtc cacatggcat cgcgcaaggc cggcgcaatc 6601 ggccgggccg ggggccggcc gccgtggagc ttcggaccgc tccgggcggc cgactcgggt 6661 tgcgtcgagg tccaggccgc gaggcggttc cggacctcct cctcgcggcc ggaggccgcg 6721 agcttctgct gcgcgtagac gcgcaccgtc tccagcatcc ggaagcgtga gctgcccgct 6781 ccccgcaagg gggccaggag cgacttgccg accagccggc cgacggtgtc gaccacctcc 6841 gcgccggaca gggtcgtgtc ggcgcagaag tgttccgcgg cgtcgaggtc ccaggtgccc 6901 acgagcacgg acaagtcgtg gaagacccgc ttctcgtcgg cgccgaggag ttcatggctc 6961 cagtccagcg tcgcgtacag gttgcgatgc cgctcgggag cggggaccgg ggcctgagcg 7021 aggatatgga ggccgtcggc gatccgcgcc tcgatgtccg ggacggtgag catgcgggtg 7081 cggccgacag ccagttcgat ggccagcggc aggcagtcca gccgacggca caccgctgcc 7141 gcgctcgccc gcgtgacgtc atcccacccc aggtccggcg tcgccgcggt catccgggcc 7201 cggaacagct caaccgcctc gccgagcggc aacgggcgca gcgggaaggc cgtttcgccc 7261 ggcacggcca gcgcctccct gctcgtggcc agcacggtca gagcggagca gccgcgcatc 7321 agggtgtcga cggtgtgggc cgcgccctgg cgtacgtgtt cgcacgtgtc gaccacaagg 7381 agcatccgct tcccggccag ggcggccttc agtcgcgcga gttgaggaat ccggtcgggc 7441 gaatccggga tgccgagcgc ctcggcgaat gcggcgatca gccggtcggg gccggcaccg 7501 gcgggaagtg aggcgagttc caccaggcag atgccgtccg ggaattcctc gcgcagattg 7561 tctgccgtct ggagggcgat ccgggtcttg cccacgccgc ccggaccgac cacggtcacc 7621 aggcggttct gccgcaccgc ttccgtgatg gcccccaggt ccgcgcgccg tccgacgaac 7681 gcgtcgagcg gcgggacgag tccgcccgtc cggcccgcct caccggtggt ccgggaacct 7741 gacccgggaa ccacccggct cggggcgttc cggggccccg gcggcggcgt cgtggcggcg 7801 ggcccccttc gcgggacgtt cagtgcgtgg gtgtcgtggg ccagtaaagc ggcgtgcagt 7861 gcgcgcaacg gaggggaagg actcaccccc agcgcgtcac ggagtactcc gcgcgtggcg 7921 tcgaaaacct ccagcgcctc ggcctggtgg cccgctccgt agagcgcccg catcagcagc 7981 ccgcgcagac tctcgcgcag cgggtgctcg gcgacaagcg ccttcagctc cggcaccagg 8041 gcgacgtgct gcccctgggc aagccgcgcc tccgcgaggt gctcggatgc cacgaggcgc 8101 agttcgtgca atcgcgcccg ctcaccggcg atggccacgg agtcgcccgc gccctccagc 8161 gccgggcccc gccacagccg cagtgcctcc tgcagcaccg ccgccgcgtc ggccgggcgc 8221 ccttggtcga ggtggtcgtt gccctcggcg gcgagccgtg cgaagcgggt cgcgtcgagc 8281 atgtccgccg agagccccat gcggtatccg aggcccttcc gggtctgcag caggccgctg 8341 ttcctgccca gcaggacgcg cagcttggct atgtgcaggt gcaaagcgtt gaccgggtcc 8401 ttgacgggtc ggccggccca taattcctcg atcagaaaat cccgtttcac caccgcgggc 8461 gcttggacga gcagcagagc cagcagagtc gacaaccggg cgccccgaag ggggagggcg 8521 gcaccgtgct gcatcagcat ctgtaccggc cccaggacct ggaagaacat acgaacagta 8581 tccagagcgt tactgtccag agcgatcagc tctcgcccgt gatgccgtag ttgcgtctca 8641 acgtccacaa attactagtc atcttcatag gacaacagat cgagcacaga ccgcggcggc 8701 agcgggacag gcgcgccaca gagccacgac agaaccgttt gctgggattg ctgcacagcc 8761 caacgccacc caccagattc aggcagggga gggtcccatg cacacgtcgt ggagctccgg 8821 tcggccaccg cgcccgcagg atcgccgatg accgccgcgg tgccgccccg ggtggaggcg 8881 gccgggagcg cgggatgggt acgccaggtg ctcgccgccc gctgggggcc gcggtggtcc 8941 ggtgtccccg tgtcggccgc tctcggccgg tggcaccgcg ccgctcagtc acaccgcggg 9001 cctgtggtac gtggagcgcc ccgagggggg ctacgtactg aaggtccagc tgaatcccga 9061 cgccgcacgg gagccgcggt tctatccgct caaggaagag gtcctcgcgc actgccgcag 9121 ccacggggtg cccgccccgg cggccgtggc ggcctcggac ggcgccacca gcgtctggca 9181 cgacgggctg gtgtgcgaac tcgctccgct cgcgccgggc gccgcgtccg gcgagctcac 9241 gcccgcccag gctgccgccg tcgtacgcac cgcgctcggg ctgcgccgcg ccctggaccg 9301 gccgccggac ggggtggcgg acaggctcgc cccgatccgg ctgccccggc tggtcgacga 9361 ggaacactgg ccgtccgccc tgcgggacgc cgatgagcgg ctgctgccac tcgcagagcg 9421 gcgcggcgac agctggggcc gggcggccgc ctcggcgctg cgcggcgtca tcgcgtcggg 9481 tcggctgctg cgagaggccg gaccggccgc ggcgggcgaa cagcccgcca ggcccgcggt 9541 ggtgcacggc gatctgcact accaccactt cctgttctcc cccacgggcg acggtgaggg 9601 cgaaggcgat ggccctgacg tcgtagccgt gctggacttc gacaatctgc acgtcggcga 9661 ccggttgctg gacctcgcct ggctggccga gatggcgggg cgggtggccg gcgagaccag 9721 ccgccgccgc tccctcgccg cgttccgcga ggcggcgacg gccgccggcc tgctcgcgcc 9781 gggcgaggag cggctgctga tgccgctcct gatggcccac tcgctgccgg tgatcgtcga 9841 catcgccaag gacatcctgc tcagggacat cctcagcccc gcgtggaccg agtacttcga 9901 cctgctcgca cccccgcgca ggcttcggat ccaccagtcc ctcgtctctc cgctcccgcg 9961 ctgagcgcgt cgggcacacc ctcacagaga gaggttcctc atgtctgtcc aagagtccac 10021 gctgtcgtcg tggccgaccg acctgtccgc ctactgcttc gaggtcccgc actccgagga 10081 cgcggtgctc ttcacctccg gcaagggaat ccggctgcag gacgccggcg gccgcagcta 10141 tctggacggc ctgtcgggcg tgttcgtcac ctgcttcggc tacgactgcg agcccatcaa 10201 gcgggcggtg gaggaccagc tgcggacgct ccccttcaac ccacccctgc acggcaccaa 10261 tcacgccgca ctggagctgg cccggggcct ggtcgacctg gcgccgcccg gcatcacggc 10321 ggccaagctg gtcaacggcg gttcggagag tgtcgaggcg gcggtccggc tggcccggct 10381 ctaccaccgt acgcacggca atcccgacaa gatcaaggtg ctcagccact accacgggta 10441 ccacggcgcc accttcggct cgctgtcgct gaccgggcgt cccgatgtcg ggcgcttcgg 10501 cccgggcctg ggcggcttcc tccacgtctg gccgccggac tgcctggagt ccttctgggg 10561 cgtgccgccc gaggagtcgg gagcgctggc cgcatcgatc atcgagcgga cgatcgaggc 10621 cgagggcccg gattcggtgg ccgccctggt catcgagccg gtcatccatc tgcgcggcat 10681 ggcgatcccg tcggccgact acctcacgcg gcttcgggag atctgtgacc ggcacaacgt 10741 gctgctcgtc ttcgacgaga tcgtcaccgg attcggccgc accggaaagc cgttcgccgc 10801 ccagaccttc ggcgtcaccc cggacatcat gtgcgtcggc aagggcatat ccggcggtta 10861 cgctccgctg gccgcggtgc tgatggccga gcgaatcgcc gcgattctcg gtgactccgg 10921 cggtccgctg gccttcgccc cctcgcacac gtacgccgcc aaccccatgg cctcggccgc 10981 gggcctggcc gcggtgaacc tgttccggga cggcgacttc ccggtgcgga tcggcaagct 11041 gtcggagtac atcgagccac ggctgcggga gctgatcggc gggcgcggca ccgtgcggtc 11101 gatcggactg ctgtacgggg tcaaactgct ggaggacggc ggcgaacggc cgatcggcga 11161 acggttcgcc cgggcgtgcc tgcagcgcgg cctgatcatt cgcggtcagg acgactggtt 11221 cgtcatggct cccgccttcg tgacgaccga gaccgaggcg gacgagatgc tgcgcgtcct 11281 cgccgccgcc ctcgacgagg tgtgcgggag cggtcgatga ccgggctgag cagtctcttc 11341 gccgagccgg gtgaggccgc gctgccccgg ctgacgcccg aggagaacaa ggtggtgcgg 11401 cgcgagctgg ccgagctgga agcgggcgat tacagcggcc cgctgctcga ctcgctgcgc 11461 aagctcgcgg ccgccgttcc cgagcgggtc catgacgaca gcgcccgggt cctgctgcgg 11521 atgcacgaga tcgcgggctc ggggaactac cagagttgcc tgtcgtccct gccgctcgta 11581 cgcgcctgct tcgagctcgg actcgtatcc gtggactccg aggagggcca gggcccggcc 11641 gagctgatcg tcggccgggg acacatcgcg ccgagcttct acgccgagca ctacgtccgt 11701 ggcaccttcc cgttcacacc gctggccacc ctgcaccagg gcggactgac cggggtgatc 11761 caccgggacc tgggcttccg caacaccatg cggtacagcc tcggtgtggg cgtcgcgcag 11821 gcggtgagtc tcgccggcgc gatggccgag cggggcgaca accgcaaggt ggtctgcctg 11881 gccggcgacg gcgagttgca ggagggcgtg gtcttcgagt gcctgcgctt cgcccacgac 11941 gcgggtctga ccaatctggt cctggtcgtg gacgccaacg acatgggcat cgagccgctc 12001 ccgaagccgc tcagccgcga ctacctggcc ggctatctgc accgcgtcga ggaggtcgac 12061 ggcctggacg cgaaggccgt ctcggactcg ctaggcgaac tgctggccgc gccggggtcg 12121 gcggcgctgg tgtgccggac ccgcaagggc gaccacagtt tcaagccgaa gccggccgag 12181 ggctcggccg caccggcgaa gcccgccaag gtgtccttcg ccgcgaccac cggacggatg 12241 ctggccgacc gccaggagcg caccggccgg gaactcgcgg tgttcaccgc cgacatggcg 12301 aaccgcttcg gactgcgcgg caaggtgccg tacaccaaca ccgggctcgc ggagacgctc 12361 agcctcggcc tgaccctcgc gctgcccgag cggacgctga aggtggtcgc caccgacgcg 12421 atgtactaca tggactcgct cagcatgctc accgaggcga ccaccagcgt gcgcaatctg 12481 ctggtgctgg ccgggcgcag ctggggggcc tggggcggcg cgcacaacgc cgcgaacctg 12541 ctcggcctgg tgatgaacac ccgcgtctac gagccggtga ccgaggcgga gttcgacgcc 12601 tgcctggacc ggctggacca caacccggac atcacgcacg ttctcagcac ggtcgacgcg 12661 aagttcgacc cgcccggggc ggactgctcc gccgacgtgg acggcggagt gtggatcacc 12721 ccgcccgccg ccggtgacct ggggactgcc ctggtcacct tcggctacgc gaccgtactc 12781 gtcgcggagg ccaaccggga cctcggcctg ccgcatctgc actgcgcggc gctcgacccc 12841 gacctcgacg cggagaccgt cgcgcgcttg cgcggcagcg aacggctgct ggccatcgag 12901 tacaacgggg cgcacggcgg cttcggcgag cggctgcgca cgaagtacct gctcccggcc 12961 gaggtgcacg gcgtacagcg ggacatcgcc aaccgcgtgc acgacaagca gctccggctg 13021 caccggatga gcccggcgca gctgcgcgaa ctgctcgacg gaaggccgtc ggacacggac 13081 ggcgaggacc gtcccgggga ggaggcccgc cgtgcggctg cacgcgcatg actaccgggc 13141 gccggacacc tcgggcatcg tcgacggcgc catcctcatg catcgtgagg tggccgaact 13201 gctggccggc caccggagcg tggcctggca cgatctgacc cgcggcgccg agagtgtcgc 13261 atcggcctcg ccgggcgagg tggtgtacgc cggggcaggc ccgtacgcct tcctgcacca 13321 tctgtggcgg gagcgtacga agggccgctt ccggatcgtc cgcgaggtgc acacggcgct 13381 gtggtccggc tactgggcac aggaggagct gtgcgcgccg ctgctgcgcc ccggtgacct 13441 ggtgctgttc ccgaccgagt acacgcgccg gctgttcatc cggcatctgg cgtcggtcac 13501 cgaggacaac tcggcggtcg cctaccccat gctcgaccgg ctgccccggc ttccccgggc 13561 ccggataccg gcgcccgacg agccgctgcg catcggctat ctcggtgctc tgtccctggc 13621 gaagaacttc gaccaggtgc tgtcggtctt cgctcgctgc caccgggcga gcccggggcg 13681 cgtcagtctg ctgtgcgcgg gcaagccgaa ccatccccgc tgggagaccg atgcggtgct 13741 ggcggagctg ggccggcagg gggtcgagcc ggatgccgtc cggatgctcg gcgtcctggg 13801 gcagaccgaa ctcgccgggt tcttctcgca gatagacgtg ctgctgttcc cgagcacggc 13861 gtcgcgggag acgctcggca gggtggtgct ggaggcgctc gcccacggga ttccggtact 13921 gggcgccgac atcggcccgg ccgtcgaact gctgcccgcg cgcaatctgg tgccgacggt 13981 gctcaacacc gggaccgcgt tctccatgag cagggtcgag gcgctcggct gggtcgacga 14041 ggacgccctc gtggacaagc tgcgcgcccg cgacttcgaa cccgcggatg tgatcgccga 14101 gtcgccgtac caggaccgga cgttcctgcg ggcgctgtcc ggcgaggcgg tgcgacagga 14161 gatcgcccac gacgcccggc tgaccgatcg gctgacggtg gagcggcgga agggcgtcga 14221 cctggaccgg gcgctggcac ggtccgagca gatcttcacc gacttcttcc ggcaccggga 14281 cgacggcctg ctgtccgcgg tggacgccct gccggaggag ccggcccaag gccgccggca 14341 gctgcggcgg ctgatcaccg aaggaagccg caatctggcg gactaccggg ctttcccccg 14401 gctcctggac gccctgttgc tgcccccgct cgactacggc ctgacgccgg agcccgtaag 14461 aacgacgcat cggaaggaca tgacggcatg acgacgacgg aacgcgggac cgggaccgat 14521 acggggacag ggacggggct cgccggcaag gtcgcgctgg tcatcggcgg gggccggggc 14581 atcggcgccg ccgcggtgga ggcgctggcc cggcaggacg cgaccgtggt ggtggccgac 14641 accgaccggc tgccctcggc tttcaaccac taccggtcca cccgggtgag cgggttcgcc 14701 gacgcccaga agctggcggc ccggctgagc gaggcgggac tgcgggtgac cgcggcggag 14761 gtcgatgcca ccgacgaggc cgcggtgcgc gagctgtacg ggtcgctggc aggcgggccg 14821 gggcggctcg acgtggtcgt caacgccttc ggggtgaccc atgtcagccc ggtggagcag 14881 atggagctca cggagttcca gcaggtggtg tccggcaatc tggacggggt cttcctgtcc 14941 tccaagcacg ccgtgccgct gctgcgcgac tccggcggcg ggtcgatcat caacttctcc 15001 tcggtgtcgg ggcgcagcgg cttcgccaag gtggcgcact actgcgccgc gaagttcggg 15061 gtgatcggct tctcctcctc gctcgccctg gaggtggccc gcgacggcat ccgggtcaac 15121 gcgatctgcc ccgggatgat ccggaccaat atgtggagct atctcctgtc ggcgttcgtc 15181 cggcccggcg agaccgagga cgagtgctgg gagcggatgc gcaccatgat tccccagcgg 15241 gagttccaga ctcccgagga catcgcggag ctggtggtct tcctggccac ggcgaagaag 15301 gtcaccggcc aggcgatcag cgtcgacggc gggatgacgg catgacggcg acgcagccca 15361 cgggcgccgc acaggcggag gtggtgttcg gcttcgacga gggctggagc tggtcggccg 15421 agccgctctc ctgctcgcgt accggcgggt cactggagat caccgcgcca ggtggggccg 15481 atctgttcgc catgcccggc gcccacgagg cgagcggact gccgctgctc cagcgcacgg 15541 tccggggcga cttcaccgcc tggacccgga tctcggtcga cggcgccgcg ttcggcgacg 15601 cgggcgggat cgccctgcac ggccccgagg gctggttcaa ggtctgcgtg gaacgggtca 15661 aggcgagtgg ctgggcggtg gtcaccgtgc tgtcccggcc ggtgtccgac gaggcgctgg 15721 ggccggcgct ggccggtccc gaggcggagc tgctggtgac ccgcgagggg ccccggcagg 15781 cggtgttctt ccgtgaggcg cccgaggacg actggcggtt cgtacgaacg ttcttcggct 15841 tctcggagcc ggagctgcgg ctggggctct tcgtccaggc tccgttcacc ccctccgcct 15901 cagccgtctt cgccccggtc aggctccgcc ccacggccct gcgcgaccgg aggtgaccat 15961 gccggtgccg cccgccgcac ccgcccgggt acgcgtcgtg gccccgctgg tgctgtcctt 16021 gctgctgggt gtggtcaccg cggcgctggg agcggcgttg ccgctgctca ggtccgcgta 16081 cgggctgccc gaggggagcg gggccgagct ggtcgggctg tacaacttgg gtgcgctcgc 16141 cggggtcgcc gcgtgcggtc ttggcggccg tacccccgcc ggggggtcca ccaccgggcg 16201 cccggtcctc ccgctgctgg ccgcgttcgc gggcggctgc gcggggatgg cgctcgcgcc 16261 cacctggtgg ctgctgaacg cgttcgccac ggcgacgggc ttcggcttcg gcgggctgct 16321 gctgtatctg aacgccttct tcggccgctt cccggggcgg cgcgggctgc tgctgatgaa 16381 tctgctgcac gcgtgcttcg gcgcgggcgc caccgtggga ccgctgctgg tgggccggtt 16441 cggtgaggtg ccgtggctgc tgctggtgac ggcggggctg acggcgccgc tcctctggcc 16501 gacccggcac ctcggcacgg cgggccccgg cgccccaccc acgcccgaca ccggcaccga 16561 cgccccggcc gggcgccgga gcctgcccct gctggccgtg ttcgcggccg tggccttcct 16621 gtacgcggga gtggaggccg gtaccggcgc gctggagtcc acccacctgg aggccgcggg 16681 ccgttccgcc gagagtgccg ccgggctcac cgcgctgttc tgggcgggcc tcaccgccgg 16741 ccgggtggtg ctgccgcccg tgacgcaccg gctgggtcca ccgcggctgg tcctgctctg 16801 cctgctcgcc gccaccggcc tgctcctcac cacgctcgac gaccgctggg caccggcggg 16861 ctacggactg gccggcttcg cgctgggcac ggtcttcccg accgtgctcg tctgggccac 16921 cgccgccctg ccgctcccac cggagcgcgt cagcgccgtg ctgctgctga ccaatctgat 16981 cggcagcgct gccctgccgc ccctgctggg cctgttcgct gccccggccc gggcagccgt 17041 gtcgattccg ctgtcgatcg ccactctcac cctgctgtgc ggcctggccg tcacggccgc 17101 cgtcgccctg gcccggcggg cggatccgac cgccgcccgc cgtcaccccg accgacccga 17161 ccgacccgac cgacccgacc gacccgacca gaaggtaagg acatgacctc accgcagccc 17221 gtcaccgagt tgttctgggc gtactggaag accaagacga tgctcagcgc catcgagctg 17281 ggcgtcttca ccgagctggc caaggagccg ctgcgcggag acgagctggc cggccggctc 17341 ggtctcggcc ccggcgccgt cggcgacttc ctggacgccc tggtggcgct cgacctgctg 17401 gaccgtaccg gcgatgtcta cgccaatacc cccgcggccg acgaatacct ggacgcgggc 17461 aagcccgcca cctatctcgg cgacagctac ctgacccagt gcgcgggcct ggccgacgac 17521 ctgacgggag tgctgcggac cggcgaggcg ctgcgcgggg cccgtaccgg ccgggacttc 17581 tacgccgaca cgtacgcgac gtcgaactcg gtccgcgcct tccagcggga catgacggcg 17641 ctgagcatcg gctcggcacg ggtgatcgcg gacagcttcc cctgggacga ctaccgctcg 17701 ctcgccgaca tcggcacgtc cgagggcgcg ctgcccggcc ggatcctgct gcgccacccg 17761 cacctgaccg cggtcggctt cgacctgccg caggcccgcg aggggttcga ggagtacacc 17821 ggcgcgctcg acctcgccga ccggatgagc ttccagcccg gggacttctt ccgcgacgaa 17881 ctgccctcgg ccgacgtgct ggtgttcggg cacatcctgc acaactgggg gctggaggag 17941 aagcggctgc tgctggacaa ggcgtaccgt gcgctgccgg agggcggggc cgtgctgatc 18001 tacgagacgc tgatcgacgg cgaccggcgg agcaacgcgg tcgggctgat cctcagcctc 18061 atgatgcacc tggaggtccc cggcggcttc gactacaccg gtcccgagtg ccaggaatgg 18121 ctgcgtgagg cgggcttccg ggagacccgc gtggaacacc tggacggtcc ggaatccatg 18181 atcatcggat tcaagtgagc agggcgggca cacagccggc gacggccgcc gcgagcgacc 18241 tcgtcatgtt ctggaacctg tacgaggcgg gcatcgaccg gctggaaggc cgggacgaac 18301 gctggctgct gcagcgggac atcgtcgcgg aacaccggcc ccgggtactg atgaccaccg 18361 aggggtgggg ctgggaccgg gacggggggc ggttcttcga ggacgccaag gccgggttcg 18421 gcatggacgg cgcgctgttc accgcgaaga ccggctgcca tctggcgatc ttctggcagc 18481 gggacatcga accagtggag accgagcgga caccgcacga cctcgccgcg tggcacggcc 18541 acggcagcgc cacgctgcgg ctgcccgggt ggtcgcacga gctgcggttc gtcgtggctc 18601 atctggaccc gttcagcccc acgaaccggc ggatcgagtc cgaccggctg cgccggtacg 18661 cgaaccccgg tgcgccggcc cccacggtca tggccatgga cgcgaactcc ctcccgccgg 18721 acgatccgga gccggactgg acgaccgtgc cccgccaccg ccgcgacgac caccggtggc 18781 ccggcgagaa gcacgccgac cgcgctccgc tgcggcggct gctgggcgag gccgaggatc 18841 cgctgttcgt cgacgcggga gcccatgcgc gggatcgctc gccgaccttc ggacaccacc 18901 cgccgggcca ggcgccgcgc cgcatcgacg tgttcctgct gtcgccggcc ctgaccgggg 18961 cactggcctc gtaccaggcg gtggacgacc cccggctgct cccgcacgac ggacggccgg 19021 gcgcctccga ccaccggccg atcaccctcc ggctccgccg ccggtaaccg gacctgagga 19081 aggggctctc catggacgat gacagggaag cgaccgacga gcggggacac ctggacgcgc 19141 tcaaggcccg gatggccggc gggctgccgt acgtgtccac cgagccggcc ctgatgccct 19201 accgggagcg ctgtgcccgg ctcctggacg cgttcaacgc gaccgcgctc accgacgacg 19261 aggcccggca gccgattctt cacgagctgc tcggccgggt cgggcccggc ggctgggtga 19321 tgccccggtt cctgtgcgaa ttcggcttct tcatcgagct gggcgccgag atcaggatca 19381 acttcgacgc cgtgcttctg gactgcgcgc cgatcgtgct ggggtcgcgc gtctggctgg 19441 cgccgaggtg ccagctctac accgccgacc acgacctcga ccccgggcgg cggagcgcca 19501 tgtgggagcg ggcccggccg atcaccatcg gggacgacgt gtggctcggc ggcggggtga 19561 tcgtgctgcc gggggtgacc atcggcgaac ggaccgtcgt gggcgccgga agcgtggtca 19621 cgaaggacct gccggccgac gtgatcgcgg cgggcaaccc cgcccgcgtg atccgcgagg 19681 tgccgcggcc acagccgtga acccactccc cccttgacgt acccacgaaa ggttcctgat 19741 ggacccagcg atccaggcca tcgtcttcga ccacgacgga gtgctggtcg actcgatccg 19801 tccccacttc ctcgcctgct ccgccctgtt ccgggagcac ggcgcggaac tcccccaggg 19861 ctggtgggcg cgggaggtct gcggcagccc cgacgcgcac cccctgctgt tcggcatgct 19921 gcggaactcc gccggggccg ccaccggtac cgacgcccag ctcgaggacc ggctggaggc 19981 gctgtgggcc gagcacttca cccccgagaa cgtgcggttg atgccgggag tccgcgaact 20041 gctcgccgcc ctgcggaccg cgggtctgcc gctcgcggtc gcctcgtcgg cggacgaggg 20101 ctgggtccgg cgctggctgc gccactacga actcgacggc agcttcgcga cggtcgtcac 20161 cggtgaccag gtaccgcgac ggaagcccga ccccgccgtg tacctggaga cggccgcgcg 20221 gctttcggtg gcgccgcggc gctgcgtggt gttcgaggac tcggtgagcg gcgtcgcggc 20281 ggcccgggcg gcgggcatga cggtgctggc cgttcccacc ccgctgacgc gtgcttgcga 20341 ctactcgctc gcccaccggg tactgcccga tctgaccgtg gtggatgcgg cggagctgtt 20401 cctcgcggat ccggcccggg ccgggcagcc gtgacggcac gaacggcgcg acccgcgcgc 20461 tccgtacggt ctgtgcccca ggtcccggcc tggtggcgga gcgcggtgat ctacgagatc 20521 tacctgcgca gcttcgccga tggcaacggg gacggcacgg gcgacctcgc cggggtccgg 20581 gcccggctgc cgtatctggc gcggctcggc gtcgacgccc tgtggttcac gccgtggtac 20641 ccctcgccca tggccgacgg gggctacgac gtcgtggact accacggtgt cgacccgctg 20701 ttcggcaccc tcgacgaggc ccagcggctc atcgccgaag cccttgagct gggcatccgt 20761 acggtcgtgg acatcgtgcc gaaccacgtc tccagcgccc atccgtggtt ccgcgcggcg 20821 ctcgccgcgc ctcccggctc cgcggagcgc gcgctgttcc acttccgtcc cgggcgcggc 20881 ccctcgggtc aggagccgcc caacaactgg cgttcggagt tcggcggccc ggcctggacg 20941 cggaccgccg ggccggacgg aagccccggc gagtggttcc tgcacctgtt ctcccccgac 21001 cagcccgacc tcaactgggg ccacccccgg gtgcgcgacg cccacgagga ggtactgcgg 21061 ttctggctga accgcggggt cgcagggcta cgcgtggact ccgcgaccct gctcgccaag 21121 gacccggccc tgcccgacct cgccgacccc ggcctcgccc cggagggccc ctcgcacccg 21181 tacgccgacc gcgaggaact ccacggcctc taccgctcct ggcggcggat caccgactcc 21241 tatcccggcg accgcgcgct catcggcgag atgtggctgc acgaacccgg gcgcttcgcc 21301 cgctatctgc gccccgacga gctgcacacc gccttcaact tcgactttct gcgcagcccc 21361 tgggacgccg gacagctgcg ccgttgcgtc gacgccacgc tcgccgcgca cggcggcgcc 21421 cccgcctgct gggtgctgtg caatcacgac gtgacgcgca ccgtcacccg ctacggccgg 21481 gcccgcaccg gcttcgactt cgccgccaag gcccacggca cgccgacgga tctcgccctg 21541 gggacccgcc gcgccagggc cgccgcgctg ctcaccctcg ccctgccggg atccgtctac 21601 ctctaccagg gtgaggagct gggcctgccg gaggtcgagg acattccggc ggaccggctg 21661 cgcgatccga tgcacttccg ctcgggcggc gaggatccgg ggcgcgacgg gtgccgggtg 21721 ccgctgccgt ggagcggtgc cgctccgccg tacggcttca cggatgagga cgccgagccg 21781 tggctgccgc agccgaaggg atgggcggag ctcaccgtcg aggcgcagga gggcgcggcc 21841 ggatcgatgc tggagctgta ccgggaggcg ctgcgcctgc ggcgggcgct gcccgcgctg 21901 cacggcgcgc cgctggagtg gctgcccggc ggccccggcg tgctcgcctt ccggcgcggt 21961 gacgtggtgt gcgtggtgaa cctctcggac cgcgccgtcc cgctgcccga ggggcacacc 22021 gtcctgctga ccagcgagcc gggaccgccg ggcggcgccg cgctcgctcc ggacgcggcg 22081 gcgtggacgg tgcaggacgg gaaggacggc ggacatgcgt agcgcggcag tgatcggaac 22141 cggcctcatc ggcacctcgg tggcgctcgc cctcaccggc cacggggtca ccacctacct 22201 cctggaccgg gatccggagg cggccaggac cgccgcggcc ctgggcgccg gcctcgccgc 22261 caccccgcgc gccccggtcg acctggcggt cgtcgcggtg ccgcccgcgc atacggcgtc 22321 ggtgctcaag gagtatcagc agcgcatggt ggcgcgtttc ttcaccgacg tggccagcgt 22381 caagacgcgc cccctgatgg acgccgcgtc ggccggctgc gacctgacgg cttacgtcgg 22441 cggacacccc atggcggggc gggagttgtc gggcccgctg gccgcccgcg cggacctctt 22501 ccaaggccgt acgtgggtgc tcaccccctc cgcgttctcc cgcgccgagg ccctgcacca 22561 cgccaccgag ctgatcgagc tgtgcggcgg ccggccggtg atcatggggc acgccgagca 22621 cgaccgggcg gtcgccctga cctcgcacgc cccccacatg atggcgagcc tgctggccgg 22681 ccggctgctc ggtgccgaga cggcacagct gcggctggtg ggcaaggggc tgcgggacgc 22741 gactcgcatc gcggcgggag acgccgcgct gtgggccgac atcatcggct cgaacgcggg 22801 ccccgtcgcg cgggtactgg cccaactggc cgaggagctc ggcgcggtcg tcgcggcgct 22861 gcgcgagctg gacgactccg ccgcacgtcc cggcgggaag ggggcggcgg accttctggc 22921 ggcgctcgcg cggggtgagg aaggacggtc acggatcgtc gccgccgagc ggccggccga 22981 cgcggcgcgg gccgcccgct gaacactgcc aaccggcaca ccaccgaagg gagccaggat 23041 gaacgacgaa atcgtgttca tcgtcggaga ccgcttggcc ggggggccgc cgcgggcggg 23101 cctgatgccg ttgtccaggc tcatagcgga cttgagagcg gggcggctga gggatccgct 23161 gccggcgctc gtcccgggac agggcatcga acggcacgag atggacctca tacgggcgga 23221 gctgaagcgt tgccggctgc ccgggaccgc gctgtgggac gcggtcccgc cggagctcgc 23281 cggccgcggt gaggtccaca agcaccgtca ggagaacgtc ctgatcgccg gtctgcggcg 23341 gatcggcgac acccactacc aggccgcgct gcgcctgtcg aacacgcacg agatggtgct 23401 cgaccacacc accggcaccc atgtcacggg aatggtggtg acggaggccg tccggcagat 23461 gtcgctggcc gtcgccgaga ggtatctgct gaagccgggg cagcccgttc cgcggttcct 23521 catcaactcc cttcaggtgt cgttctcccg cttcctgctc ccgctggatg ccgagatcga 23581 ctatcacctc gacgaggccg tgtggaagcg gccggacgtg ctgcgctacc acgggcagtg 23641 cgacgtcctc caggcgggcg tcacgaccgc gtccggttcc atgagcctgt cggtccacga 23701 ggagggcgcg gccgagcgga tcgaggagac ccagagcgac gccgtcgtcc ggcggctggc 23761 cgatcgccat caggccggcc ggtcgccgga accgtccgag caccgcgtcc cgtgacccgg 23821 ccaggaggtc gaccatgaca tggcttctgc ggagatttcc gctgttcgtg ctcaccgtgt 23881 ccgtcatggc cacggcgttg gccaccgtcc gtgaggcggc cggctggtcc gacccggcgc 23941 accgggtcgg atgcgccctc gcggcgtcgt atctgctgtg gctgctgctg gagagctgga 24001 cgacggtgcg cagttcgcga gcgaccgagg cgacgaccga ccgcggcacg gtctacgcgt 24061 acggcgcggc gcgggccgtc accgtggcga ccgcgtgcct ggtgccgacg gactggtccg 24121 gttaccggcc gtggatgccc gtcctgccgg tggccttcgt ctgcgccgtc gccttgcgcc 24181 tggcggcggt ccgggccctc gggcggttct actcgcaccg ggtgcgggct ctcggcgacc 24241 accggatcgt ggacaccggc ccgtaccgcc tggtgcggca tcccgcgtac gtcggcatgg 24301 cggccgcgca catcgccttc gtcggctact tcctcaaccc cgcgagcgcc gccgcgctgg 24361 tggtcctgct gctgccggcc atcgtggtgc gcgtcctggt cgaggaacgg atgctgttcg 24421 ccctgcccgg ctactccgac ttcgcggcgt cccggcgccg tctcatcccc tgggtgtggt 24481 gacatcggca tgacgatcga gaacgtactg cgggccgtcc gccccgccgg gcgccgtggc 24541 ccggtactgc gcgggagagc actgctggac gaggcggagg tgggcccgaa gttcgcccgc 24601 caggcccgga tggcctcggc cggactgccc gtgccccact tcttctgcct gcccgcgagt 24661 ctgttcaccc gcaccctggc acccgtacgc ccgcggctcc tggcgatcct gcggcggctg 24721 gaccacaccg accccgaccg gctggccgag cagtccgacg ccctgcgccg cctggtcctc 24781 gacgtgccgg tcccgccgtc ggtggcgcgc gccgtccacc gggcgtacga cgaggtgttc 24841 ggcccgcacg ccgtggtgtc ggtgcggtcc tcgatggtgg ggccgcgcgg gcgggccgcc 24901 gaggactcgg ccgaggacgc cttcgcgggc atcggcgaaa gctttctgta cgtcacccgc 24961 gatcaggtgc tggagcgggt gcgcgcgtgc tgggcctccg ggttcggcac ccgggcactg 25021 ttgtaccggg cggcgcgctc caccgccgcc acggaggact tcgccgtctg cgtgggcgtc 25081 cagcgcatgg tgttcggccg ccgctccttc gtcctgttca cctgcgaccc gctgaccggg 25141 tcccgggacc gggtactggc ggccgggctc ggcatcgggg agggggtcgt ccaggagaag 25201 gtgccggtgg accatcactt catcgaccgg cgcacgggcg cggtccgctc cgtcgtcgcg 25261 cacaagtccg agcgcatgga ggaggatccc gcgcggccgg gcagcgggcc ccggctgctc 25321 ccggtgcacg gccgcgagcg ggacgaaccg gcgctcaccg acggccagat ccggcacatc 25381 cgggacgtcg gcgaccgcct cgagcggctt ttcggctgcc cgcaggacat cgagggcacc 25441 ctcaccgagg acggcgccct acacatcctc cagacccggc cggtcgtcct cgacctgggc 25501 gggcagcggc tgtggagcaa cgcgaacatc accgagagct accccggtgt gaccaccgcc 25561 ctcacctact ccttcgccca gcgcttctac cgtgaggact tccgcgactt ctaccggcgg 25621 ctcggggtct cccccgccgt gctggcacgc cacgagaacg acctgcgatc catgctcggg 25681 ctgctgaacg gccgggtcta ctactccctc agcgcgtggt acacgctgca ccggctgtcc 25741 cccacgttcc cgctgtggcg cgccagtttc gagcggatga tgggcatgtc cccctccgtg 25801 acggatgtcc gccccgaccg cctcgccacc tctcccggcc ggctgccgga aaccgccgcg 25861 gccctgctgc gcctggcctg gctgcgggcc ctgcacgtcc ccgccgcgcg gagcttcgac 25921 cgctggtggc agcagaccat cgcgcgccac cgcgaggccg ccaggaccgc cgacccgctc 25981 caactggcgc ggctgatgcg gtcactgtgg caggaggccg gggagcgctg gggcctcacc 26041 ctcgtcaacg acgtgctgtt gcagaccacc gagaccgcca tcatgcggct cttctcccgc 26101 tggctgcccg gcgccgacgc cggtctgcac agcgacctgc tgtgcggtgg cgaggaaaac 26161 cgcagcgtca ccatcctgat gtccctcgtg gatctcgccg aacggatcag ggccaccccc 26221 gggctgctgg acgccctcgc cgacgaggac cccggcaagc ctcccgagga gctgctgcgg 26281 cggatcgagg acggcgcgtt cggcacggac ctcgcagacc ggatgcgggc ccacgcggcg 26341 gcccacggcg accggggcct gcaagagctc aagctggagg tcccggtgcc gcgcgaacag 26401 ccttcccagc tcctgcggct ggccggcgag tacgcgcgcg gcggcctgac ccgcgagtgc 26461 ctgcgcggcc gggagaccgc gacccggcgg gccgccgagg agcggctgcg gacgctgctg 26521 gcgggccacc ccgtgcggcg ccgggtgctg cgcaccctgc tcgcccagca gcgccggtac 26581 atcaacatcc gggaggacac ccgctacagc cgcagcgagc tgttcggatt cggccgccag 26641 atctaccgcc ggctgggtac ggaactcgcg gcgcgcggtg ccctggacgc ggcggaggac 26701 gtggtccacc tcaccgagga ggagatcctc ggccacttcg acgggggcgc ggtcaccgac 26761 gatctgcggg ggctggcgcg gctgcgccgg gccgagtacc tgagccgtgc caccctcgat 26821 gccgaactcc ccccggactt cgccacgatg ggcccggtac gcgacgggct gcccactcct 26881 ggcggggacg cgcggccggg ctcgcggctg cgggggctgg gctccagcgg cggtgtcgtg 26941 cgtggcaccg cccgtgtggt catcgacccg caccagcgtg tcgatcccac cgacgacctg 27001 atcctggtgg cccgggagac cgaccccggg tggctgttcc tcatgctctc cgcgcgcggc 27061 atcgtcgtcg agcgcggcac cctgctgtcg cacaccgcca tcagcggccg gaagttcggc 27121 atccccacgg tcgtcgcgct gcccggcgcg acgagaagga ttcccgacgg ggcacgcgtg 27181 gagatggacg gggcgaccgg taccgtgacg atcctggacg aggtggcgga atgagcggca 27241 cggtgcggcg gctgtacgcg ttcttcgccg agcgcgcccc cgcccgcgtc tatgtgacgt 27301 acgcggtgct gtgggtgctc gccctggagg gtgcgctggc cctggtggag ccgggcgacg 27361 cccgctggat gctgggggtg cccacgctcg ctgagatcgg cacggtgtgg ctggccctgc 27421 tcttcatccg ggtcgtggac gagcagaagg acctgggtta cgaccgggag cacaatccgg 27481 gcaggccact gccgcgcggc tcgatcacgg tacgcgaact gcgactcgcc atggcggtca 27541 tcgcggccgc gggcatcgca ctgaccgtgt ggcggtcgcc cctgctcacc ggctggctgc 27601 tgctggatct cgcttacatc tgtttcctgg tcggactcga acgctggtcg aggtcggtcc 27661 gggacggcat cttccgcaat ctggcggtga gctatcccgt gcaggcgctg ctcagcgggc 27721 acgtgtggct gtcgtaccgg gacgcgacgg gcgccggccc gcactggagc gccgccgtgc 27781 cggttctgct ctgcgcctgt gtcttcctgc acttcgagtt cgcccgcaag accagccacc 27841 gggtcacgcc cggcgccacc ctctactcca atgccatcgg cacccggggc tccgtcgggc 27901 tgaccgtggg ctgcgccctg gcggcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnccg cgctgagcgt ggcggacccg ggcttgaccg 28141 tgtaacaccc cgcgccccga ccgcgtcaca ccgccaacgt ccgaggatga ggaccacccc 28201 atggccgcac ccgtacccgt cccgtccacc caccgcgagg tgcgcctgac cgcgcgtccc 28261 gggggcggac cgcgccccga tcggttcgag gtcgccgagg ttccggtccc ggtgccccgc 28321 cacggccagg tcctcgtacg caatcggctg acgcgggtcg gcgcggtgac ccgcacgctc 28381 atggaggacg ccgccggcgt cctcccgatg tccccgtacg caccgggccg ggcgctgtgg 28441 gccccggcgc tcggcgaggt ggtcaccgcc ccgggcaccc gcctgggccc cggcgatctc 28501 gtcaggcacc agctcggctg gcgggaatac gcggtgatcg aggcggacga cgcacggcgc 28561 gtcgacccgg ccctgtggcc cgacccggcc gcctgtctct cgcagggatt cgcgggctgg 28621 ctggcggtgg tacgcgccgc cgcagtacaa ccgggcgaca cggtgttcgt caccggcgcg 28681 gcgggagggg cgggctccat cgccggacag ttcgcccgcc tgcggggagc gggacgggtc 28741 atcgggtcga cggggtcgcg gcgcaaggcc gactacctcg tggggactct gggtttcgac 28801 gcgaccgtcg tcaggggcga ggcaccgatc gaggagcagc tgcgcgcggc ggcccctgag 28861 ggtatcgacg tgctcgtcga cacggtcggc ggcgagcagc tccgggccgc cctggcactg 28921 gcccgccgag gcgcgcgatg cgtcgtcatc ggcgcgctgt ccgcccaggc ccgcggcggc 28981 acgacggccg aggtgccggt cgacaccctg gcgctgttct ccgggggcat caccctccgc 29041 gggctcacca ccgccgacca ccgcgacgcg gtgccgcgct gggaggagca gttcagccgg 29101 ggcctgcgcg ccggtgtcct cacctttccg cacacccggc tgcgggggat cgaacgggcg 29161 ccgcgtgccc tgggcgaact gctggacggc caccacatcg gtacggtcct cgtggagtcg 29221 tgaccggaag aacggcggcc ggtttcctcg tcctagccga agaccccacg accgagggac 29281 catggaggct cggccgtggg gccggtgttc cggccggggt gccggaaccg ccgtgaggag 29341 gtcggggatg accggatctg ccaaggggat gacggcgtgc gcggtgggtg gcctggtagc 29401 ggtgacggcc tacaccgtgg cgctggggag caatggctgg ctgtggttcg cctgggtggt 29461 gctcgcgctg ctgacggtgg ggacgctgct cacccgctga gcgggtgcgg cctcacgccg 29521 cggtgccgcg cagccagcgc cacagggacg tccaccacgg gacgcgggcg cgcggcgccg 29581 cggtgccgtc ggtggcgtct tcgcccgcgg ataaggcctc gtcctcgtcc gcgggcaccc 29641 agcgcgtggg gcgcggcttg ggcgcgagca gcatgccgga ggtgtccacc ggatcggtct 29701 cggccctgcg cggcatgaac gcgaccggca gcgcgaccag atggcgggag atccaggacg 29761 cgatccagtt cagctcggtc tccggcacgt ccagccgcag atcgggcagg cgcagcagca 29821 gggtgtcgat ggcggtgtcc gcgatggcgc ggccgatgtc ctggccgggg cattcgtgcg 29881 ggccgccgct gaacgacaga tgcgagcggt tgccgtacat gggtgcgccg aggtcggggc 29941 ggatggcggg gtcgacgttc ccggcggcga ggccgaggag cagcatgtcc ccggccttga 30001 tctgccgtcc gcccagttcg gtgtcgccgg tggcccagcg gccggggatc acggtgaggg 30061 gcggttcgtt ccacagcacg tgctcgatgg cgtccggcag ggtcatatga cctccggcca 30121 gggaggcgcg gaagcgcggg tcggtgagga ccatgcgcag cgtgttggcc atgaggttgg 30181 tggtggtctc gttgccggtg aggagcacga gccgcagatg ctggacgacc tcctcctcgg 30241 tcagctcgga gggatggctc agcagccagg acgcgaggtc gctgccgggc tgggtgcgtt 30301 tgcgctcgac gagctggcgc agggtgtcgg tgacgtactg gtcgctgcgc agggccgtct 30361 cggagccctt catcaggtcg cgggtggcct cgaccagccg tgggccgtac tcgtcgggca 30421 tgccgagcag ctgggtgagg acgagcatgg gcagccgctg ggcgaagccg gtgaccagtt 30481 cggcctggcc gtcggcgcag aagtcgttga ccagctggtg ggtgaagcgg gtgatgtggc 30541 ggcggatgcc gcggcggtcg accctattca tgctgtcggt gagcgcggcg cgcagccgct 30601 cgtgctcgtc gccgtcggcg aaggtgcaca tgggctgcca gccgaccacg gggagcagcg 30661 gcgagtcgtc cgggatccgg ccctcccccc aggcgctcca gtaccgggag tcgcgggaga 30721 agcgggaggg ggtgcgggcg acctccaggt tctcgcggta gcccagcagc agccaggccg 30781 gtacgtcgtc gccgagccgc accggggcca ccggtccgtg ttcgctgcgc agccgctcgt 30841 acaggcccat cgggtccttc tcgttctcgg atccgaagag gtccaccaca ccggcgaggg 30901 ggctccgggc gtgggcgggg cactggtcgg ggggaggggt cacggtgtct ccggggtcac 30961 ggtggtgagg tgttgcagat agctcacgag ggtgaggagc gtgtcgcggc tggatccgcg 31021 gtcgcgggcg tcgcagtcga tgatggggac ggcgtcgggc aggtccaggg ccgcgcgcag 31081 ctcgtcgacg gggtggacgg gcgccccggg gaaggcgttg atggcgacga cgaaggggac 31141 gccccgctcc tccagccggc ccaggacgtc gaagctgacc tccagccggc gggtgtcgat 31201 gaggaccacg gccccgagcg cgccctcgaa gaggccgttc cacaggaacc agaagcgctc 31261 ctggccgggg gtgccgaaca ggtagagcac cagctgttcg ttgaggctga tgcggccgaa 31321 gtccatggcc acggtggtct cggtcttgcg ctccaccccg gcgatgtcgt cgacgccgac 31381 cccggcctgg gtcatcgtct cctcggtggt cagcgggcgg atctcgctca ccgagccgac 31441 catcgtggtc ttgccgaccc cgaatccgcc gacgatcacg atcttgacgg ccgcggcggc 31501 ggaggccggg agggcgtcct cgctgcgggg gccgacgagc gtctcagagg ttctgtagtc 31561 catgcatcac cgcctggagg agttcggtgt cgggcagtac cgcggccggg accggtgccc 31621 gcgcctccac ctggcggtgg gcgagcagat ccgcgaggag cacggtcacg gtgctgacgg 31681 gcagcgtcag atacgcggag atctcggcca cggagagcgg gaagtggcac atccgcagca 31741 cggcggcggc ctcgggcagc atgcccggct cgggttcggc ccgggccacg atcaggctga 31801 ccagttcgat ggtgtccgcc cgctgggacc ggccggggcc gccgtcgacg aggtagagcc 31861 gttcggggcc gccctcgtcg gtgccctttc cggtccggct catggcatgc gtccgctggc 31921 ccgtggcggg ctgctgaggt gttcgccgag ccgggcgacc aggtctctca tgcactggcc 31981 catcagtccg gcgtccacgc cgtcctcggc gagcaccgcg aggtaggagc tggtaccggc 32041 cgccatcaga tagaagaagc cgccgtcgac ctcgatgacc accagccgca tccgcccgtc 32101 gccggtgggg aattcgacgg cg //