LOCUS       NZ_GG657754            10861 bp    DNA     linear   CON 01-MAY-2024
DEFINITION  Streptomyces himastatinicus ATCC 53653 supercont1.1, whole genome
            shotgun sequence.
ACCESSION   NZ_GG657754
VERSION     NZ_GG657754.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces himastatinicus ATCC 53653
  ORGANISM  Streptomyces himastatinicus ATCC 53653
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
REFERENCE   1  (bases 1 to 10901646)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Annotation of Streptomyces hygroscopicus strain ATCC 53653
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10901646)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q.,
            Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z.,
            Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A.,
            Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L.,
            Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S.,
            Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 10901646)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q.,
            Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z.,
            Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A.,
            Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L.,
            Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S.,
            Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Arachne v. April 2007
            Genome Coverage                   :: 6x
            Sequencing Technology             :: ABI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000158915.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 03:02:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,680
            CDSs (total)                      :: 9,608
            Genes (coding)                    :: 8,720
            CDSs (with protein)               :: 8,720
            Genes (RNA)                       :: 72
            rRNAs                             :: 4, 1 (5S, 16S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 888
            CDSs (without protein)            :: 888
            Pseudo Genes (ambiguous residues) :: 0 of 888
            Pseudo Genes (frameshifted)       :: 423 of 888
            Pseudo Genes (incomplete)         :: 530 of 888
            Pseudo Genes (internal stop)      :: 36 of 888
            Pseudo Genes (multiple problems)  :: 97 of 888
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:01:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 992537
            Orig. end                         :: 1003398
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GG657754.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation was added to the scaffolds in August 2010.
FEATURES             Location/Qualifiers
     region          1..10861
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="6"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10861
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10861
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5861](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5861)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            684..2198
                     /locus_tag="SSOG_RS03575"
                     /old_locus_tag="SSOG_00730"
     CDS             684..2198
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056770.1"
                     /locus_tag="SSOG_RS03575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00730"
                     /product="NADH-quinone oxidoreductase subunit NuoF family
                     protein"
                     /protein_id="WP_106516588.1"
                     /transl_table=11
                     /translation="MIKTKPRLACVDPPRLLAGLDEAYRLDRVGHLTVHGTMPALRADE
                     LVALAENIDLRGRGGAGFPFAKKVTAVVEAAGRAGGRCNVVVNGSEGEPSCLKDTALLL
                     HTPHLVIDGATLAAEAIGAEEVVIACHRPDVEESLNAAIAERGPSGVPVEVSVTPDRFV
                     AGEGGAVINGISGAPAIPNGRKIRSSENGLGGLPTLLSNTETYAQLAVAARMGALPYRS
                     VGLPTEPGTTLLTVGGKYVMETPTGVPLTYVLELCGLTPGQGVLVGGYHGKWLDPKSIG
                     AATISRESMKKYGARLGAGAVLPIPEDTCPLGETARVARWLAAETAGQCGPCFIGLPAL
                     ADAIGQVERGGGQSSIDNVRAYINSVRKRGACSHPDGTAGFVTTALDAFPEEFESHAMG
                     AGCGRPTIGVLPMPEETQPLALPPGPAVAPPRAERQQRGPMEKLLVDWSLCQGHGLCAD
                     ILPPEVLTLGIDGYPSSARMEVPRQLRNQAVRAVRRCPALALRIEE"
     misc_feature    2281..2290
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.9"
                     /tool="antismash"
     misc_feature    complement(2378..2389)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.06"
                     /tool="antismash"
     gene            2454..>3173
                     /locus_tag="SSOG_RS03580"
                     /old_locus_tag="SSOG_00731"
                     /pseudo=""
     CDS             2454..>3173
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040526.1"
                     /locus_tag="SSOG_RS03580"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00731"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLTAACGSNDTSAGSANVQPVGGSSQTASAGASASAGAGAAAAGA
                     GATGGYGSDTGAAAGSGEAGGSQTGPAKELAVKSDAKLGKFVSDSAGMTLYRFDEDTPK
                     PAKSNCNDACATKWPPVPADDATAGAGIDSKKLGKVKRADGSEQLTLEGWPVYRYAGDT
                     KAGDTKGQGVGGTWNALAPDGKPAGKKAGGGALELMVSNNADLGKIMVNGEGMTVYRFD
                     KDSAWPMKDGNPPTPPAL"
     gene            3235..3396
                     /locus_tag="SSOG_RS57870"
     CDS             3235..3396
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /locus_tag="SSOG_RS57870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_342634411.1"
                     /transl_table=11
                     /translation="MTSFTRPDGTKQAAFDCWLLYTFAGDKKPGDTNGQGVKGTWFALD
                     DKGKKVTQ"
     misc_feature    complement(3270..3279)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            3479..3988
                     /locus_tag="SSOG_RS03585"
                     /old_locus_tag="SSOG_00732"
     CDS             3479..3988
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181496.1"
                     /locus_tag="SSOG_RS03585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00732"
                     /product="hypothetical protein"
                     /protein_id="WP_009712842.1"
                     /transl_table=11
                     /translation="MSRVLRAAALGAALLCAGGLVSGCGGSSHADGKKKEKAHAAKELG
                     KPGKPAALTQIADAIGCKATIVTEADELRQGACQSSKGDRYTMVTFATAKGQKDWLNTS
                     KDYGGMYLVGTRWSVTGQSSGSLKPLRAKLGGAIEQGSMMHHGGSKSQGGSESGGGKEH
                     DGMKMD"
     gene            complement(4017..4694)
                     /locus_tag="SSOG_RS03590"
                     /old_locus_tag="SSOG_00733"
     CDS             complement(4017..4694)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056767.1"
                     /locus_tag="SSOG_RS03590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00733"
                     /product="HAD family phosphatase"
                     /protein_id="WP_039936484.1"
                     /sec_met_domain="HAD_2 (E-value: 5.5e-22, bitscore: 77.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADTLLAWTPAAIVFDCDGTLMDSERHWEKAREVVLADYGAVCD
                     EEFAGRTKGLHYTECGRMMAEFAGLPDLADEMTRQLLSAFRRLVAEDPVTMPGAAELVH
                     KAAAFAPLAVASNCPRDVVEDGLSKAGLLRYFDHVLVPGGEVRPKPHPDVYLAAARLCG
                     AAPGDALAVEDSHCGFLSASRAGLRVLGVGPRKPVDERPPVDLWVSTLADPDLLKWAES
                     RAA"
     misc_feature    4847..4867
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(5001..5861)
                     /locus_tag="SSOG_RS03595"
                     /old_locus_tag="SSOG_00734"
     CDS             complement(5001..5861)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151398.1"
                     /locus_tag="SSOG_RS03595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00734"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_245606214.1"
                     /sec_met_domain="AfsA (E-value: 8.4e-19, bitscore: 66.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAREAVHRVSVAEVLLTDVRTLGDGRFEAAAQWPRSHPTFPHGGD
                     GRHHPLMIAETLRELGIYIPTRYYAVPAGAHFLIRDLSFRLDPETEPRAPHGATDITCR
                     IAVTDVRTAQPTGRFVSGMRLDVGLWAGGRIFARAGGTARFLDGTAYAETRSAHSGQTA
                     RRRLRAAVRPSPGQLGVPAARDVLVVRDGGVVYLDPADPRHPFFFDHWSDHVPGLVLLE
                     GARQAAALATGGLLIRPLACRMKAIRFTESYPPAVVECVSHGRTCVFRLRQADICTAVG
                     VLQYG"
     misc_feature    complement(5938..5951)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.6"
                     /tool="antismash"
     misc_feature    5939..5952
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.9"
                     /tool="antismash"
     gene            6047..6679
                     /locus_tag="SSOG_RS03600"
                     /old_locus_tag="SSOG_00735"
     CDS             6047..6679
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056765.1"
                     /locus_tag="SSOG_RS03600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00735"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_039940587.1"
                     /transl_table=11
                     /translation="MQERAEQTRRSLLEAAASLFDERGYAGTSISDITARSGHTSGAIY
                     FHYTSKERLALAVVQEHFATWPPLIDRHTGLHVPALEQLVRLSFAVARAFRDDIVVRAG
                     ARLWTERSLIAAPFPPPFIGWIDAVGAMLERAHGEGELAAHVDPLPAARTIVCAFFGLH
                     TVSEALDGRRLVEDHLAELWTLLLPSLRAAPGHPGHTADLLAAARGA"
     gene            complement(6692..7492)
                     /locus_tag="SSOG_RS03605"
                     /old_locus_tag="SSOG_00736"
     CDS             complement(6692..7492)
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181492.1"
                     /locus_tag="SSOG_RS03605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00736"
                     /product="endonuclease"
                     /protein_id="WP_009712846.1"
                     /transl_table=11
                     /translation="MTSVTRWKSLALLAGVVTVAVTAQTPATAVPTSTTAYDDTYYKDA
                     IGKTGEPLKDALNQIISKDVSKLSYDEVWDALKKTDEDPDNSANVILLYTGRSQSKNSN
                     GGNADDWNREHVWAKSHGDFGTSTGPGTDIHHLRPADVSVNSTRGNKDFDEGGSEVGEA
                     PGNRTDDDSFEPRDQVKGDVARMILYMSVRYEGGEGYPDLEPNDKVDNGSQPSMGRLSV
                     LKKWNDEDPPDAFEKKRNQVIYDDFQHNRNPFIDHPEWVADIWK"
     gene            complement(7630..8031)
                     /locus_tag="SSOG_RS55575"
     CDS             complement(7630..8031)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /locus_tag="SSOG_RS55575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_245606215.1"
                     /transl_table=11
                     /translation="MWRIHVQAATVLADALVDRVRDGGRLVLVGSRTMTGVPGKSQYAA
                     TKAALTGLARSWAAELAPRGITVNVVAPGPTDTPMLRDPGRSRTPPVLPPLGRLVRPEE
                     VAGLTSFLLGPEGGAVTGQTLVMCGGASL"
     gene            complement(7929..8315)
                     /locus_tag="SSOG_RS55580"
     CDS             complement(7929..8315)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (5-92). E-value: 5.4e-07. Score:
                     21.6. Matches aSDomain:
                     nrpspksdomains_SSOG_RS55580_PKS_KR.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /locus_tag="SSOG_RS55580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_245606216.1"
                     /transl_table=11
                     /translation="MAEHAVVTGVSSGIGAAIATRLLDEGWRVTGISRTAPPHAHDRLR
                     WLPADLARPEELPQALTPMDEDGPLDALVHAAGVQRAARLANWTPRTGPSCGASTSRRP
                     PSWRTRWWTGSGTAAGWCWWAAGR"
     aSDomain        complement(8040..8300)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SSOG_RS55580_PKS_KR.1"
                     /evalue="5.40E-07"
                     /label="SSOG_RS55580_PKS_KR.1"
                     /locus_tag="SSOG_RS55580"
                     /protein_end="92"
                     /protein_start="5"
                     /score="21.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVTGVSSGIGAAIATRLLDEGWRVTGISRTAPPHAHDRLRWLPAD
                     LARPEELPQALTPMDEDGPLDALVHAAGVQRAARLANWTPRT"
     gene            complement(8308..9513)
                     /locus_tag="SSOG_RS03615"
                     /old_locus_tag="SSOG_00738"
     CDS             complement(8308..9513)
                     /EC_number="3.2.1.18"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056763.1"
                     /locus_tag="SSOG_RS03615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSOG_00738"
                     /product="exo-alpha-sialidase"
                     /protein_id="WP_009712848.1"
                     /transl_table=11
                     /translation="MTADHDTAIPLTDGRLRPRPGDPARQEAFLPAPAVQNHAANLTVL
                     PGGDLGCVWFGGTQEGVPDISVWFSRLAPGADTWSEPVRLSDDPARSEQNPLLFPTPAG
                     ELWLLYTAQQAGNQDTAEVRVRVSGDNGDTWDAPRTLFPATGGGGVFIRQPIAVLDSGR
                     WLLPVFHCVSTPGAKWVGDHDTSAVMVSDDEGRTWRERPVPGSTGCVHMNVHPLPDGTL
                     LALFRSRWADAVYRSHSTDGGESWSEPEPTELPNNNSSVQYVPLGDGRLALVYNHSSRA
                     DATARRVSLYDEIDDDGEAGDAPAAAPSQREDAPGAFWGAPRAPMTLALSADGGRTWPV
                     RRDLDVGDGHCLTNNSRDRLNRELSYPTIRQTPDGTLHIAYTYHRQAIKYVRVSPDWAA
                     HG"
     gap             9846..10523
                     /estimated_length=678
     misc_feature    complement(10836..10848)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
ORIGIN
        1 ttcctcgact tcggcgccgg ggtgctcgcc ctggtctgcc tcagctcagc ggtgctgtgg
       61 gggctcgccg cgaccgacca actgatcctg cactccaacc accggctcat cgcccagggc
      121 atccaccggg cggccgcggt cgccggcctg ctgtttctcg gcgttcacat ctgggtgaag
      181 gtcgccgaga gccacacgaa cgcagcttcg atcgtgctac cgttcgctga cgcaaagcag
      241 ccggtgctga tcgggctcgg aaccattgcc ggttacctct tcgtcatcgc ggcggtgacc
      301 ggagcggtcc gcagctcgtt cgccgccgcg ggcaactccc gctggcggta ccggtggtgg
      361 cgggcgctcc atgtgggcgc ctaccccgcc tggggcctcg cgctggtcca tggtctgaag
      421 gccggacgtg aggccaagat ctgggcgaca tacggctaca tcgcggctct ggccggagtg
      481 gccatcgcgc tgtggatgcg gctcgcagcc cggcgcagcc aggacgacga tcgaggggac
      541 ggcatctcgg tgaggaatcc gcccgcccgc ggccttcagg aaggccgcac ccggcgcgcc
      601 gcgcccactg tgccgcggcc ccgttcggcc gactcgggcc gacgggacgg tgaacggctc
      661 ggtgcctccc gtgggtggga ccggtgatca agacaaaacc caggctggcc tgtgtcgacc
      721 cgccccggct gctggccggt cttgacgagg cgtaccggct cgaccgggtc ggccacctga
      781 cggtgcacgg cacgatgccc gccctcaggg cggacgaact cgtcgcgctc gcggagaaca
      841 tcgatctccg cgggcgcggc ggggccggtt tccccttcgc caagaaggtc accgcggtcg
      901 tcgaagcggc cggccgcgcg ggcggccgct gcaacgtggt ggtcaacggc agcgagggcg
      961 aacccagttg cctcaaggac accgcgctgc tgctgcacac cccgcacctg gtcatcgacg
     1021 gtgccaccct ggccgccgag gcgatcggcg ccgaggaggt cgtcatcgcc tgccaccgac
     1081 ctgatgtcga ggagtcactg aacgccgcca tcgccgagcg cgggcccagc ggggtcccgg
     1141 tggaagtgag cgtgacgccc gaccggttcg tggccggtga gggcggcgcc gtcatcaacg
     1201 gcatcagcgg cgccccggcc atccccaacg gccggaagat ccggtccagc gagaacggtc
     1261 tcggcgggct gcccaccctg ctgtccaaca ccgagaccta tgcccagctc gccgtcgcgg
     1321 cgcggatggg cgccctgccg taccgctcgg tggggctgcc caccgagccg ggcacgacac
     1381 tgctgacggt cggcggcaag tacgtgatgg agacgccgac cggggtgccg ctgacctacg
     1441 ttctggaact gtgcgggctc acccctggcc agggcgtgct ggtgggcggc taccacggca
     1501 agtggctgga cccgaagagc atcggcgccg ccaccatctc gcgcgagtcg atgaagaagt
     1561 acggcgcgcg gctgggcgcg ggcgcggtgc tgcccatccc cgaggacacc tgcccgctcg
     1621 gcgagaccgc gcgggtggcc cgctggctgg ccgccgagac ggccggtcag tgcggtccct
     1681 gcttcatcgg cctgcccgcc ctcgcggacg cgatcggcca ggtggagcgg ggcggtgggc
     1741 agtcgtccat cgacaatgtg cgcgcgtaca tcaactcggt gcgcaagcgc ggcgcgtgca
     1801 gccaccccga tggcacggcc ggattcgtga ccacggcgct ggacgcgttt cccgaggagt
     1861 tcgagtccca tgcgatgggc gcgggctgcg ggcggcccac gatcggcgta ctgccgatgc
     1921 ccgaggagac ccagccgctg gccctgccgc ccggcccggc ggtcgcgcca ccgcgcgcgg
     1981 agcggcagca gcggggcccg atggagaagc tcctggtgga ctggtcgctg tgccagggcc
     2041 atgggctgtg cgcggacatc ctgccgcccg aggtgctgac gctcggcatc gacggctatc
     2101 cgtcctccgc ccggatggaa gtgccccgtc agctgcgcaa ccaggcggtg cgcgcggtgc
     2161 gccgctgccc cgcgctggcg ctgcgcatcg aggagtagcc ccccgccccc ctctaagtct
     2221 ccctccccac gacggccgtt gcgcaacggc cgtcgcttgt ttgtgcgccc tcgtacccag
     2281 cgttttttgc gtctggtgac aaatttttaa ctggctgaac gtccctcggc acgggctccg
     2341 tatgggcatg gtgtcggcgc cttcaccgcc ggacaccgat aaggatttcg cagggagtgg
     2401 tcatggagaa gcggcgtcag atcgcattcg cgggaatgtc cgcggcgctg gtcatgctga
     2461 cggcggcgtg tggcagcaat gacacctccg cgggcagcgc gaacgtccag cccgtgggtg
     2521 gcagcagcca gacggcgagt gcgggtgctt cggcgtcggc gggtgcgggc gcggcggccg
     2581 cgggggccgg cgcgaccggt gggtacggca gtgacaccgg cgcggcggcg ggcagtggcg
     2641 aggcgggcgg gagtcagacg ggcccggcga aggagctcgc ggtcaagagc gacgccaagc
     2701 tcggcaagtt cgtgtccgac agcgcgggga tgaccctcta ccggttcgac gaggacaccc
     2761 cgaagcccgc caagtccaac tgcaacgacg cgtgcgccac caagtggccg ccggtccccg
     2821 cggacgacgc cacggccggt gcgggcatcg actccaagaa gctcggcaag gtcaagcgcg
     2881 ccgacggcag cgagcagctg accctggagg gctggccggt ctaccgctac gcgggcgaca
     2941 ccaaggcggg cgacaccaag ggccagggcg tgggcggcac ctggaacgcg ctggcccctg
     3001 acggcaagcc ggccggcaag aaggccgggg gcggcgccct ggagctgatg gtcagcaaca
     3061 acgccgacct cggcaagatc atggtgaacg gcgagggcat gaccgtctac cggttcgaca
     3121 aggacagcgc gtggccgatg aaagacggca atccaccgac gccgcctgcc ctggacaagt
     3181 ggaagccggt caaggccatc gacaccacca aggtctccgg ggtgaaggac gaaggtgacc
     3241 tccttcaccc gccccgacgg caccaagcag gccgccttcg actgctggct gctctacacc
     3301 ttcgccgggg acaagaagcc cggcgacacc aacggccagg gcgtcaaggg cacctggttc
     3361 gcgctcgacg acaagggcaa gaaggtgacc cagtaagacg gcatgatggt ccatcccgac
     3421 gggggcccgg agcggcggcg cttcgggctc cccgccggac gtcgacggga gggttgaagt
     3481 gtcacgcgta ctccgtgccg ccgcgctcgg tgcggcattg ctctgtgcgg gcggcctggt
     3541 gagcggctgc ggcggaagca gccatgcgga cggcaagaag aaggagaagg cgcacgccgc
     3601 caaggagctg gggaagccgg gcaagcccgc ggccctgacg cagatcgccg acgccatcgg
     3661 ctgcaaggcg accatcgtca ccgaggcgga cgaactgcgc cagggggcct gccagtccag
     3721 caagggcgac cggtacacca tggtcacctt cgccaccgcg aagggtcaga aggactggct
     3781 gaacacctcg aaggactacg ggggcatgta tctggtcggc acccgctggt cggtcaccgg
     3841 tcagtcctcg ggatccctca agccgttgcg cgcgaagctc ggcggggcca tcgagcaagg
     3901 ctcgatgatg caccacggtg ggtcgaagtc ccagggtggt tccgagtcgg gcggcggcaa
     3961 ggagcacgac gggatgaaga tggactgacc ggtgcaccac ggtccctctc tcaggctcaa
     4021 gccgcccggg attcggccca cttgagcaga tcggggtcgg cgagcgtgga gacccacaga
     4081 tccaccggcg gacgttcgtc gacgggtttg cgcggtccca cgccgagcac cctcagcccg
     4141 gcccgggagg cggacaggaa gccgcagtgg gagtcctcca ccgcgagcgc gtcaccgggg
     4201 gccgcaccgc acaggcgggc ggccgccaga tacacatcgg ggtggggttt gggccgcact
     4261 tccccgccgg ggaccagcac atggtcgaag tagcgcagca gcccggcctt ggagagcccg
     4321 tcctccacca cgtcgcgcgg gcagttgctc gccacggcca gcggtgcgaa cgccgccgcc
     4381 ttgtggacga gttcggccgc acccggcatg gtgaccgggt cctcggcgac cagtctgcgg
     4441 aatgcgctca gcagctgccg ggtcatctcg tcggcgagat cggggagtcc ggcgaattcg
     4501 gccatcatgc ggccgcattc ggtgtagtgg agccccttgg tgcggcccgc gaactcctcg
     4561 tcgcagacgg ccccgtagtc cgcgagcacg acttcgcggg ccttttccca gtgccgttcg
     4621 gagtccatca gagtgccgtc acagtcgaag acgatggcgg cgggggtcca tgcgagaagc
     4681 gtgtctgctg tcatctgcct gccgttccag gagagatggg ggaatcggcg cagggggtgc
     4741 ctgcctgtag ccacggggag cagcgcggtg aattctgggc ggacgctgcg cgcggaatgc
     4801 cggatgcggc atggaaaagc cgtacaggcg gagaattgcc tgcccgaaac ataacgatca
     4861 ctacgttact gagatgactc cgggtgagca aggggtcact gagcgtcggc gatttgttcg
     4921 tcgtccagtg cgttcgtagg tcgtcgcgtt cgtcgcacgg agggttcgtc gcacggagcg
     4981 ttcgtcgtcc tggtgcgcgg tcagccgtac tggagcacac cgaccgccgt gcagatgtcc
     5041 gcctggcgca gccggaagac acacgtcctg ccgtgggaga cgcactcgac cacggcgggc
     5101 ggataggact cggtgaaccg gatggccttc atccggcagg ccagggggcg gatcagcagg
     5161 cccccggtgg ccagcgccgc ggcctgccgg gcgccctcca gcagcaccag ccccggcaca
     5221 tggtcgctcc agtggtcgaa gaagaaaggg tggcgggggt ccgcggggtc caggtacacc
     5281 actcccccgt cccgtaccac cagcacgtcc cgggccgccg gtacgcccaa ctgccctggt
     5341 gagggccgta cggcggcgcg cagcctgcgc ctcgccgtct ggcccgagtg ggcggagcgg
     5401 gtctccgcgt aggccgtgcc gtcgaggaag cgggcggtgc ccccggcccg cgcaaagatc
     5461 cggccacccg cccacaggcc gacgtcgagc cgcattccgg acacgaagcg cccggtgggc
     5521 tgtgcggtgc ggacgtccgt caccgcgatc cggcaggtga tgtcggtcgc cccgtgcggg
     5581 gcgcggggct cggtctccgg gtcgagccgg aaggacaggt cgcggatcag gaagtgcgcg
     5641 cccgcgggca cggcgtagta ccgggtcggg atgtagatcc ccagttcgcg cagcgtttcg
     5701 gcgatcatca gcggatggtg acggccgtcc ccgccgtgcg ggaacgtcgg atgggagcgg
     5761 ggccactggg cggccgcctc gaaccgtccg tcacccagcg tacggacgtc ggtgagcagc
     5821 acctcggcca ccgagacccg gtgcacggcc tcacgcgcca cggtgcggga ccagctgagc
     5881 ggccgaccgg tgggaccggt cggctcggaa aggccgcgag cctcgcgaac gatcaccata
     5941 aaatactaat aatgtttgac ctgtctgtcc tcatcaccat cagcggccga aagtccgcta
     6001 agttcactcc gtcacaccgg accggctgat gaaggacgac gaacacgtgc aagaacgggc
     6061 cgaacaaaca cgccggtctc ttctggaagc ggccgcatcc cttttcgatg aacgcggata
     6121 cgcgggaacg agcatcagcg acatcaccgc ccgttcgggt cacaccagcg gcgccattta
     6181 cttccactac accagcaagg aacgactcgc cctcgccgtg gtgcaggaac acttcgccac
     6241 ctggccgccc ctgatcgacc ggcacaccgg gctccatgtg cccgccctgg agcagctcgt
     6301 ccggctcagc ttcgcggtgg cccgcgcctt ccgcgacgac atcgtggtac gggcgggggc
     6361 ccggctgtgg accgagcgct ccctgatcgc ggcgcccttc ccgcccccgt tcatcggctg
     6421 gatcgacgcc gtcggggcca tgctggaacg ggcccacggc gagggcgagc tggccgccca
     6481 cgtcgatccg ctgcccgcgg cccggaccat cgtgtgtgcc ttcttcggcc tgcacacggt
     6541 gtccgaggcc ctcgacgggc ggcggctcgt cgaggaccat ctggccgaac tgtggaccct
     6601 gctgctgccc tcgctgcggg ccgcccccgg acaccccggg cacaccgccg acctgctggc
     6661 ggcggcccgg ggcgcgtgaa gcgggaccgg gctacttcca gatgtcggcg acccactccg
     6721 ggtggtcgat gaacgggttc ctgttgtgct ggaagtcgtc gtagatgacc tggttgcgct
     6781 tcttctcgaa ggcgtccggc gggtcctcgt cgttccactt cttgagcacg gagagccgtc
     6841 ccatagaggg ctggctgccg ttgtcgacct tgtcgttggg ctccaggtcg gggtagccct
     6901 cgccaccctc gtagcggaca gacatgtaca ggatcatccg ggccacatcg cccttgacct
     6961 ggtcacgggg ctcgaaggag tcgtcgtcgg tgcggttgcc cggcgcctcc cccacctccg
     7021 agccgccctc gtcgaagtcc ttgttgccac gggtgctgtt gaccgagaca tcggccgggc
     7081 gcagatggtg gatgtccgta ccgggtccgg tggaggtgcc gaagtcgccg tgcgacttcg
     7141 cccagacgtg ttcgcggttc cagtcgtcgg cgtttccgcc gttcgagttc ttgctctggg
     7201 accggccggt gtagagcagg atgacgttgg cggagttgtc cgggtcctcg tcggtcttct
     7261 tgagggcgtc ccagacctcg tcgtacgaaa gcttgctgac gtccttgctg atgatctggt
     7321 tcagggcgtc cttcagcggc tccccggtct tgcctatcgc gtccttgtag tacgtgtcgt
     7381 cgtacgccgt tgtgctggtg ggcaccgcgg tggccggggt ctgcgccgtg acggcgaccg
     7441 tcacgacgcc ggccagcagt gccagggatt tccagcgggt cacactggtc atgtgtggtg
     7501 tcctctcccc gatttacgac cgttgttcga gccccgaaag cgtgtcagac acaggatcgt
     7561 ccaatggtga acatcatgaa gagattccgt gtcagggcca tgaccgaatc agggctctga
     7621 acccggctgt cacagcgagg cgcccccgca catcaccagc gtctgaccgg tcaccgcacc
     7681 gccctccggg cccagcagga acgacgtcag cccggccacc tcctcggggc gtacgagcct
     7741 gccgagcggc ggaaggacgg gcggggtgcg gctgcggccc gggtcccgca gcatcggggt
     7801 gtccgtcggc cccggcgcga ccacgttcac ggtgatcccg cgcggcgcca gttcggccgc
     7861 ccaggagcgg gccaggcccg tcagcgccgc cttggtggcc gcgtactggc tcttgcccgg
     7921 gacgccggtc atcgtccggc tgcccaccag caccaaccgg ccgccgtccc ggacccggtc
     7981 caccagcgcg tccgccagga cggtggccgc ctggacgtgg atgcgccaca tgagggaccc
     8041 gtcctcggcg tccagttcgc cagccgggcg gcccgctgga cgcctgccgc gtgcacgagg
     8101 gcgtccagcg ggccgtcctc gtccatcggg gtgagcgcct gcggcagttc ctccggccga
     8161 gccaggtcgg ccgggagcca gcgcagccgg tcgtgcgcgt gcggcggggc cgtacggctg
     8221 atcccggtga cccgccaccc ctcgtccagc agccgggtgg cgatggcggc gccgatgccg
     8281 gagctgacgc cggtgaccac ggcgtgttca gccatgggct gcccagtccg ggctgacgcg
     8341 gacgtacttg atggcctggc ggtgatatgt gtacgcgatg tgcagggtgc cgtccggggt
     8401 ctgccggatg gtgggatacg acagctcccg gttgagccgg tcgcgtgagt tgttggtcag
     8461 gcagtgtccg tcgcccacgt ccaggtcccg ccgtacgggc caggtacggc cgccgtcggc
     8521 ggacagcgcg agggtcatcg gcgcccgggg cgcgccccag aaggcgcccg gggcgtcttc
     8581 tcgctgcgac ggtgcggcgg ccggagcgtc gcccgcctcc ccgtcgtcgt cgatctcgtc
     8641 gtagagcgag acccggcggg ccgtcgcgtc cgcgcggctg ctgtggttgt acaccagcgc
     8701 cagccgtccg tcgccgagcg ggacgtactg gacggaggag ttgttgttgg gcagctcggt
     8761 gggctccggc tcgctccagc tctccccgcc gtccgtgctg tgcgagcggt acaccgcgtc
     8821 ggcccagcgg ctgcggaaca gcgccagcag cgtgccgtcc gggagcgggt ggacgttcat
     8881 gtgcacacag cccgtggagc ccggcacggg ccgctcccgc caggtgcggc cctcgtcgtc
     8941 gctgaccatc accgcgctgg tgtcatggtc gccgacccac ttggcgcccg gggtggacac
     9001 gcagtggaac accggcagca gccaccgccc gctgtccagg accgcgatcg gctgacggat
     9061 gaacaccccg cccccgccgg tggccgggaa gagggtgcgc ggcgcgtccc aggtgtcccc
     9121 gttgtcgccc gacacccgga cccggacctc cgcggtgtcc tggttgcccg cctgctgggc
     9181 ggtgtacagc agccacagct caccggccgg ggtggggaac agcagcgggt tctgctcgga
     9241 gcgcgccggg tcgtcggaca gccggaccgg ctccgaccag gtgtcggcgc cgggtgccag
     9301 ccgggagaac cacacggaga tatccggcac gccctcctgg gtgccgccga accatacgca
     9361 gccgaggtcg ccgccgggca gcacggtcag gttggcggcg tggttctgga cggcgggtgc
     9421 gggcaggaac gcctcctggc gcgccgggtc gcccggccgc ggccggagcc gcccgtccgt
     9481 cagcggtatc gcggtgtcat ggtcggcggt catgggcgcc gggctcctcg tggtgtgcgg
     9541 tggtacgggt gtacgtcatc gaccggtcgg ggctcccgcc ccgcccctcc cccggccccg
     9601 ccggacacgg ccggtgtcgc tcgggggccg gggcccgggc ctccggcctg cggccgggta
     9661 gcggggcact gtccggaacc ccacccccgg gacacacccg gggacgctcg gggccataga
     9721 acggagcctg gggcgggggc ccgggggcgg agcccactgg tcgggggctt gggacggagc
     9781 cccacagtcg aggcccgggg gcagagcccc tggtcggggg ttcgaggggc ggagcccccg
     9841 ctcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnggaggta gaagaggtcg acgtggtcgg tgccgaggcg
    10561 gcgcaggctg ccttcgaggg ccgcgcgcat gccgcgcgcg gacagcgggg agtgctcacc
    10621 ggcgtcggcg tgcggcatcc cggccttggt ggcgaggacg atccggtcgc ggcggccggg
    10681 cagcagctcg gcgaggaggg tttcggtgac gcccccgacg tacgcgttgg cggtgtccac
    10741 gcccgtcacc ccggcgtcga gcgccgcgtc cagcatcgcc tcggcgcccg cccggtccac
    10801 cgtgtcgccg aaggtcatgg tgcccagcac cagccgggac agcggcaccg gggccccggg
    10861 c
//
