LOCUS NZ_GG657754 22065 bp DNA linear CON 01-MAY-2024 DEFINITION Streptomyces himastatinicus ATCC 53653 supercont1.1, whole genome shotgun sequence. ACCESSION NZ_GG657754 VERSION NZ_GG657754.1 KEYWORDS WGS; RefSeq. SOURCE Streptomyces himastatinicus ATCC 53653 ORGANISM Streptomyces himastatinicus ATCC 53653 Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group. REFERENCE 1 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D., Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Annotation of Streptomyces hygroscopicus strain ATCC 53653 JOURNAL Unpublished REFERENCE 2 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Direct Submission JOURNAL Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA REFERENCE 3 (bases 1 to 10901646) AUTHORS Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform, Broad Institute Microbial Sequencing Center TITLE Direct Submission JOURNAL Submitted (09-FEB-2009) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: Arachne v. April 2007 Genome Coverage :: 6x Sequencing Technology :: ABI ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_000158915.1-RS_2024_05_01 Annotation Date :: 05/01/2024 03:02:38 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 9,680 CDSs (total) :: 9,608 Genes (coding) :: 8,720 CDSs (with protein) :: 8,720 Genes (RNA) :: 72 rRNAs :: 4, 1 (5S, 16S) complete rRNAs :: 4, 1 (5S, 16S) tRNAs :: 64 ncRNAs :: 3 Pseudo Genes (total) :: 888 CDSs (without protein) :: 888 Pseudo Genes (ambiguous residues) :: 0 of 888 Pseudo Genes (frameshifted) :: 423 of 888 Pseudo Genes (incomplete) :: 530 of 888 Pseudo Genes (internal stop) :: 36 of 888 Pseudo Genes (multiple problems) :: 97 of 888 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 20:01:42 NOTE :: This is a single region extracted from a larger record! Orig. start :: 811851 Orig. end :: 833916 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to GG657754.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation was added to the scaffolds in August 2010. FEATURES Location/Qualifiers region 1..22065 /candidate_cluster_numbers="1" /contig_edge="False" /product="redox-cofactor" /region_number="5" /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /tool="antismash" cand_cluster 1..22065 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /kind="single" /product="redox-cofactor" /protoclusters="1" /tool="antismash" protocluster 1..22065 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[10000:12065](-)" /cutoff="20000" /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /neighbourhood="10000" /product="redox-cofactor" /protocluster_number="1" /tool="antismash" proto_core complement(10001..12065) /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /neighbourhood="10000" /product="redox-cofactor" /protocluster_number="1" gap 154..460 /estimated_length=307 gene <461..789 /locus_tag="SSOG_RS02725" /old_locus_tag="SSOG_00563" CDS <465..789 /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /codon_start=3 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014174678.1" /locus_tag="SSOG_RS02725" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00563" /product="ATP-binding protein" /protein_id="WP_039936400.1" /transl_table=11 /translation="PPLPAPAARIADIALDNSAKFVPSGGTVTVSLDAAGALRIADDGP GLAPEQLAAAPGRFWRAPDHGNVPGSGLGLAIAAELARSCGAELTLRSAEPRGLVVELR LPVG" misc_feature complement(506..521) /note="TFBS match to RutR, Regulator of the pyrimidine and purine metabolism, confidence: weak, score: 19.62" /tool="antismash" gene complement(1142..2077) /locus_tag="SSOG_RS02730" /old_locus_tag="SSOG_00564" CDS complement(1142..2077) /GO_function="GO:0005215 - transporter activity [Evidence IEA]" /GO_process="GO:0006810 - transport [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014174679.1" /locus_tag="SSOG_RS02730" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00564" /product="TAXI family TRAP transporter solute-binding subunit" /protein_id="WP_009712674.1" /transl_table=11 /translation="MHTVPRRLLLALAAGGLAGCSDPGPSAQVRLATGPRGGPYATFGS RLATEAHRAHHGLTVRVLTTAASVENLRMLGDGRADLALTLADSAADAAAGHGIFPRKI PVAALARLYLNYLHLVVPADSTIEHPAQLAGRTVSLGAAGSGTSTTAGRVLAAAAVRDV RAVRLGLGASADALTDGEVDAFFWSGGAPTWAISRLARRRPLRLIPLGGLARALRRDHG PVYEEVSVPAGAYGLKGPTPTVGTPSYLVCRSGLPDGTARALTDVLFERRDRLPVPDAP GSRLDERYAIGTGTVPLHPGAAAYYRAAYG" gene complement(2110..2532) /locus_tag="SSOG_RS02735" /old_locus_tag="SSOG_00565" CDS complement(2110..2532) /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014174680.1" /locus_tag="SSOG_RS02735" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00565" /product="ATP-binding protein" /protein_id="WP_039936403.1" /transl_table=11 /translation="MSSAGVDMALRQESLQLPARGASVAAARRLVRNRVPHWGFDEDVC GAAELVMSELFTNALVHTASDQILCVLRAQERRSLYVEVADQGGGPLGPMPREAGVEDE SGRGLALVRALTEAWGDRPGAAGGRVVWAQLGALAR" misc_feature complement(2713..2725) /note="TFBS match to LuxO, Repressor of luminescence, confidence: weak, score: 19.63" /tool="antismash" gene 2803..3660 /locus_tag="SSOG_RS02740" /old_locus_tag="SSOG_00566" CDS 2803..3660 /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009712676.1" /locus_tag="SSOG_RS02740" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00566" /product="helix-turn-helix transcriptional regulator" /protein_id="WP_009712676.1" /transl_table=11 /translation="MGEARSAPTVGQMVLGMRLRHLREQAGVGYDAAAKALHVNQTTVR RMEKAEVGLKLPYVEKLLRTYGVGQDEVESFLGLVEEANRPGWWHRFRDVLPDWFSLYV SLEGAASRIRAYEPHFIPGLLQTEDYARALLRIGFPQGSEEELGRRVALRMERQELLSR PGGPHLWAVMDETVLRLHIGGPKIMRAQIDRLIEAIQMPNVTLQVVPFAAGPHPGMGGP FQLFRFQIPELPDIVYAEGLTGAGYLDQRTDVAAYLEALDQMGTRATSARRTEGFLREI RKEL" gene 3663..3968 /locus_tag="SSOG_RS02745" /old_locus_tag="SSOG_00567" CDS 3663..3968 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018542876.1" /locus_tag="SSOG_RS02745" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00567" /product="DUF397 domain-containing protein" /protein_id="WP_039936404.1" /transl_table=11 /translation="MDQVEDVYNGMPATRLATEGWRKPWSGPNGGACVEALRLHDGRIA LRQSTDPDGPALIYTHHEMEKFIQGAKAGAADFLLTRPDNNNLATSAANAPERRAA" gene 3965..4786 /locus_tag="SSOG_RS02750" /old_locus_tag="SSOG_00568" CDS 3965..4786 /codon_start=1 /gene_functions="other (smcogs) SMCOG1292: hypothetical protein" /gene_kind="other" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056915.1" /locus_tag="SSOG_RS02750" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00568" /product="SAM-dependent methyltransferase" /protein_id="WP_009712678.1" /transl_table=11 /translation="MTDYGLAPDQQIDTSKPHSARMYDFYLNGKDHYEVDAEAAGKVLS VYPTVKLNATVNRGFIHRATRWLAAEAGIRQFLDIGTGIPTEPNLHQVAQSVAPEARVV YTDYDPIVLAYAQALLRGTPEGRTAYVQADVREPQRIISATELRETLDLSKPVAMSVNA LFHFIPEEEKPYELVRELMGALAPGSYLMLTHSTADFHAETWQKVIAVYKESGISFVTR SKSEVAAFFDGMELVDPGVSEPHDWKPDPGDRPDEIRSADINMWAGVARKP" gene complement(4797..6197) /locus_tag="SSOG_RS02755" /old_locus_tag="SSOG_00569" CDS complement(4797..6197) /GO_function="GO:0000156 - phosphorelay response regulator activity [Evidence IEA]" /GO_function="GO:0004673 - protein histidine kinase activity [Evidence IEA]" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_process="GO:0007165 - signal transduction [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1003: sensor histidine kinase" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056914.1" /locus_tag="SSOG_RS02755" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00569" /product="HAMP domain-containing sensor histidine kinase" /protein_id="WP_009712679.1" /transl_table=11 /translation="MRFPLRRHPRPSLRTTFALSFAAVASVVTLLVGFLSYDAAAGLVR VDQKSAFTDVIRDLRTQVRGQKVILRQYPSEVPVPYGLRDDLRRISRMDVQVLGGRGGV YDEGKPALPVGDEDRETAAAAAPGTMRQRNISIGEDDYRMATVALGDHRGAVQVAQQIS DTEDLLRELQKRTALFVVAIIVAAGLAGWWLAGRITRRLTRLTRVAESVAVTGRMEVAV PVAGDDEVGRLGRAFDGMLGRLARSKDDQRRLVQDAGHELRTPLTSLRTNISLLSRFEK LPPAARDELLADLAGETRELTDLVNELVDLAAGQRDDEPRTEVSLDEVATKAATLARRR TGRDITVRAKGDTLVEGHPAALLRAVSNLVENAAKFDRDGTGPVEVVVSGHRVEVRDRG PGIAEEDRERVFDRFYRAPGARSLPGSGLGLAIVREVALAHGGTVFARPRPDGGAVLGF TVDGPEEP" gene complement(6201..6905) /locus_tag="SSOG_RS02760" /old_locus_tag="SSOG_00570" CDS complement(6201..6905) /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_process="GO:0000160 - phosphorelay signal transduction system [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1008: response regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056913.1" /locus_tag="SSOG_RS02760" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00570" /product="response regulator transcription factor" /protein_id="WP_009712680.1" /transl_table=11 /translation="MTNTVLLAEDDRAIRHALERALTLEGYRVTSVSDGVEALAAIHRE HPDVIVLDVMMPGVDGIQVCRVLRAEGDRTPILMLTARVETADRIEGLDAGADDYVAKP FDVEEVFARLRALLRRVAPVPEPTTEGTVFDATLAVADLRLDPSARRVWRGGTELELTR TEFDLLELLARNAGIVLDHGTIYSRIWGYDFGPGSKNLAVYIGYLRRKVDQTGLTPLIH TVRGVGYTLREE" misc_feature 7078..7093 /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: weak, score: 17.39" /tool="antismash" gene 7079..8344 /locus_tag="SSOG_RS02765" /old_locus_tag="SSOG_00571" CDS 7079..8344 /EC_number="2.4.-.-" /GO_function="GO:0016757 - glycosyltransferase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Glycos_transf_1" /gene_functions="biosynthetic-additional (smcogs) SMCOG1045: glycosyl transferase group 1" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056912.1" /locus_tag="SSOG_RS02765" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00571" /product="glycosyltransferase family 4 protein" /protein_id="WP_039936407.1" /sec_met_domain="Glycos_transf_1 (E-value: 4.8e-39, bitscore: 132.8, seeds: 63, tool: rule-based-clusters)" /transl_table=11 /translation="MKIVFLLHNAYGIGGTIRTTLNLAAALAERHEVEIVSMMRHRGKP RFTLDPRVRLVPLVDTRPGGLDARDPLFAERTRDFPVHEKRNHQYNRLMDVRIARYLEG SDADVIIGTRPGVNVYLARFGPRKALRIAQEHLRYEAHSKKLRAELAPYYRELDAVVTT TEADAAVYRAKMPLPGAWTLAIPNSVPEPAAPPSEGTTPVIAAAGRLVRGKRFDLLIDA FAEVSPKHPEWRLRIYGGGSQKDRLQERIEELGLTASAELMGPYSPIEEEFAKASIVAV ASDAESFGMTIVEAMRCGVPVVSTDCPLGPAEIIEDGVTGRLIPVGDKQAMAQALRDLI DDEPGRRAMAAAALESARMYDPEPIAMRYEQLFEELASSRFARKWERTRVRLRASYRRK VRRWKRAFRRRLPAAGGTSAKA" misc_feature 7080..7094 /note="TFBS match to ZuR_variant_1, Zinc-responsive repressor, confidence: weak, score: 17.85" /tool="antismash" gene complement(8391..9881) /locus_tag="SSOG_RS02770" /old_locus_tag="SSOG_00572" CDS complement(8391..9881) /codon_start=1 /gene_functions="biosynthetic-additional (smcogs) SMCOG1001: short-chain dehydrogenase/reductase SDR" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056911.1" /locus_tag="SSOG_RS02770" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00572" /product="SDR family NAD(P)-dependent oxidoreductase" /protein_id="WP_009712682.1" /transl_table=11 /translation="MEAGRPGAEYPGAEAPGTDEPGIDPERLATCLSVLEELDRLPIDH PDAITVRRATAGIYRTVKQRRRSERRAAKTANDKAVTEATATGAADRIDDETLGRALSS SVTSEIAGILERPRSCYVCKTRYVEVDAFYHQLCRGCAAENRARRDARTDLTGRRALLT GGRAKIGMYIALRLLRDGAHTTVTTRFPNDAIRRFTAMPDSAEWLHRLKIVGIDLRDPA QVIALTDEVSAAGPLDILINNAAQTVRRSPQAYGELIAGESAPLPAGELPEAVVLGAFG SGSQTALPGPRRERDGALTAQDLTALALTSGSASPARIEAGTAIDAGGLVPDLDPTNTW IQRVHEVDPVEMLEVQLCNETAPFILVSRLRPAMAAAPARRKYVVNVSAMEGKFGRGYK GAGHPHTNMAKAALNMLTRTSAREMFESDAILMTAVDTGWITDERPHPDKMRLAADGFH APLDLVDGAARVYDPIVRGEQGEDLFGCFLKDYAPTTW" gene complement(10001..11107) /gene="pqqE" /locus_tag="SSOG_RS02775" /old_locus_tag="SSOG_00573" CDS complement(10001..11107) /GO_process="GO:0018149 - peptide cross-linking [Evidence IEA]" /GO_process="GO:0018189 - pyrroloquinoline quinone biosynthetic process [Evidence IEA]" /codon_start=1 /gene="pqqE" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: PF04055" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: TIGR04085" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: SPASM" /gene_functions="biosynthetic-additional (rule-based-clusters) Fer4_12" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014174692.1" /locus_tag="SSOG_RS02775" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00573" /product="pyrroloquinoline quinone biosynthesis protein PqqE" /protein_id="WP_039936410.1" /sec_met_domain="PF04055 (E-value: 6.3e-24, bitscore: 84.3, seeds: 518, tool: rule-based-clusters)" /sec_met_domain="Fer4_12 (E-value: 3.5e-06, bitscore: 26.4, seeds: 84, tool: rule-based-clusters)" /sec_met_domain="SPASM (E-value: 1.5e-06, bitscore: 27.7, seeds: 166, tool: rule-based-clusters)" /sec_met_domain="TIGR04085 (E-value: 3.9e-09, bitscore: 35.8, seeds: 281, tool: rule-based-clusters)" /transl_table=11 /translation="MTAHPQGHPRPWALLAELTHGCPLHCPYCSNPLQLTRRSRELTGE EWERVLRQAAELGVVHTHLSGGEPLLRRDLEVIVAAAASAGLYTQLVTSGVGLDEARLT ALTGAGLHSVQLSVQHADPGASDRIAGHRSFAAKERAAALVREAGLPLGVNVVLHRDNL DALDPLIELAVAWGADRVELANTQFYGWALRNRAALLPTREQLARAREGVARWREKLGG RTELVWVLPDYFEGVAKPCMGGWGAVSLTVAPDGTVLPCPAAAALPGLDPPNVRDHALE WIWDHSAAFNRYRGEEWMAEPCRSCSHRAEDFGGCRCQAYALTGDAARTDPACALSPDH GLIRSLTGPAPAAEPPPPGYTYRGYGRG" gene complement(11104..11370) /gene="pqqD" /locus_tag="SSOG_RS02780" /old_locus_tag="SSOG_00574" CDS complement(11104..11370) /GO_function="GO:0048038 - quinone binding [Evidence IEA]" /GO_process="GO:0018189 - pyrroloquinoline quinone biosynthetic process [Evidence IEA]" /codon_start=1 /gene="pqqD" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: PqqD_RRE" /gene_functions="biosynthetic-additional (rule-based-clusters) PF05402" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016433952.1" /locus_tag="SSOG_RS02780" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00574" /product="pyrroloquinoline quinone biosynthesis peptide chaperone PqqD" /protein_id="WP_039936411.1" /sec_met_domain="PqqD_RRE (E-value: 3.9e-19, bitscore: 67.3, seeds: 9, tool: rule-based-clusters)" /sec_met_domain="PF05402 (E-value: 3.4e-12, bitscore: 45.6, seeds: 461, tool: rule-based-clusters)" /transl_table=11 /translation="MTAPAAWRPALAPAVHLRHDRVREVDLLVMPERVVVLRGTAGTVL RLCDGGRDLDTIVAELAERFPGAPVATDVPAFLDRMHEEGWIR" aSDomain complement(11110..11349) /aSDomain="PqqD_RRE" /aSTool="RREfinder" /database="RREFam.hmm" /description="RRE-containing protein in a pyrroloquinoline cluster" /detection="hmmscan" /domain_id="rrefinder_SSOG_RS02780_PqqD_RRE.1" /evalue="1.20E-21" /identifier="RREFam006.1" /label="SSOG_RS02780" /locus_tag="SSOG_RS02780" /protein_end="87" /protein_start="7" /score="67.3" /tool="antismash" /translation="RPALAPAVHLRHDRVREVDLLVMPERVVVLRGTAGTVLRLCDGGR DLDTIVAELAERFPGAPVATDVPAFLDRMHEEGWI" gene complement(11367..12065) /gene="pqqC" /locus_tag="SSOG_RS02785" /old_locus_tag="SSOG_00575" CDS complement(11367..12065) /EC_number="1.3.3.11" /GO_process="GO:0018189 - pyrroloquinoline quinone biosynthetic process [Evidence IEA]" /codon_start=1 /gene="pqqC" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: TIGR02111" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056908.1" /locus_tag="SSOG_RS02785" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00575" /product="pyrroloquinoline-quinone synthase PqqC" /protein_id="WP_009712685.1" /sec_met_domain="TIGR02111 (E-value: 2.4e-81, bitscore: 271.7, seeds: 5, tool: rule-based-clusters)" /transl_table=11 /translation="MTRSLEERLRAVAQERYHDRHPFNLRMHAGELTPAELRRWILNRF HYQRHIPVKDALITAKLDTPRLRRMWLRRIQDHDGAADGEGGIERWLRLGEAAGIGRER LLTGAEVLPGVRLAVDGYVNFCRLGTPLEAVAASLTELSAPGIMRTRIEAFEHHYPWIE REGLRYFRDRVGQGSRDSAEALDLVLTWACTPEDEERAVAALTFKCDVLWSLLDAVDHA DTKDTAGEGI" CDS complement(12062..>12521) /codon_start=3 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056907.1" /locus_tag="SSOG_RS02790" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00576" /product="MBL fold metallo-hydrolase" /protein_id="WP_009712686.1" /transl_table=11 /translation="DADFAIAAVPVSAKRPRYAAELDGPDEDDWVVALHLRDRATGRSL LYAPALAAWSDAFQRAAEEADCVIVDGTFWDDEEPLRSGFGSRTATAMGHVPIDGPGGT AGRLAALGARTLYTHLNNTNPLNDPAAPQHAVLAGRGIEVAADGMVIEL" gene complement(12062..>12525) /locus_tag="SSOG_RS02790" /old_locus_tag="SSOG_00576" gap 12526..12863 /estimated_length=338 gene complement(<12864..12956) /locus_tag="SSOG_RS55550" /pseudo="" CDS complement(<12864..12956) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009079182.1" /locus_tag="SSOG_RS55550" /note="incomplete; too short partial abutting assembly gap; missing C-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology." /product="pyrroloquinoline quinone biosynthesis protein PqqB" /pseudo="" /transl_table=11 /translation="MRVRVLGTAAGGGVPQWNCACPGCSGGTRPP" gene complement(12968..13111) /gene="pqqA" /locus_tag="SSOG_RS02795" /old_locus_tag="SSOG_00577" CDS complement(12968..13111) /GO_function="GO:0003824 - catalytic activity [Evidence IEA]" /GO_process="GO:0018189 - pyrroloquinoline quinone biosynthetic process [Evidence IEA]" /codon_start=1 /gene="pqqA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_093467075.1" /locus_tag="SSOG_RS02795" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00577" /product="pyrroloquinoline quinone precursor peptide PqqA" /protein_id="WP_009712687.1" /transl_table=11 /translation="MNETVRQTAPEAVDHTRTPAPETAWQTPDYVVVETALEVTAYSLN AR" gene complement(13175..14677) /locus_tag="SSOG_RS02800" /old_locus_tag="SSOG_00578" CDS complement(13175..14677) /GO_function="GO:0004650 - polygalacturonase activity [Evidence IEA]" /GO_process="GO:0005975 - carbohydrate metabolic process [Evidence IEA]" /codon_start=1 /inference="COORDINATES: protein motif:HMM:NF012516.4" /locus_tag="SSOG_RS02800" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00578" /product="glycosyl hydrolase family 28 protein" /protein_id="WP_009712688.1" /transl_table=11 /translation="MTRSTRLRTAVITAAAACLLAVAPQTATAQPGAADRTYDVRDYGA TGDGTTLDDDAIDRAIDAAAAAPGRGTVLFPPGTYKSRTLHLKSDITLHLAKGATLLAA AGGMDEAEPNPWDAYQDFGHSHFRNALLTGENIHHLAITGSGTLDGEGLGTSNTLEPGV GDKILSLKRCADLDIQDVTFTRGGHFALLLNGCDGVRMTRTKVLTSTDRDAVNLINSRH VDIGQSRIEGRDDGVVLKSDYALGRTYTSQDVRVHDSDVLSTENNALQFGSETCGDFRD IRFDRLRIGGGGKAAIGIVSMDGADISDVRYSDITMTKGATPVFIKIGDRKRCPGAPPA GRIHDIRFRNVTGTGLTHPVTGVDYTSTITGAPGADVEDITFDRVRLTVPGGHPASDAE LVPGEFLTDYVPKTYGVRPSYGWWIRHAKGITFTNSAVTTDTNDDRPAFIADDGVGITL DRVTAGRGTASPYDVGFSGVTGPVVRHSTTTDGEALRIRTTG" misc_feature complement(14657..14659) /note="tta leucine codon, possible target for bldA regulation" /tool="antismash" gene complement(14674..16464) /locus_tag="SSOG_RS02805" /old_locus_tag="SSOG_00579" CDS complement(14674..16464) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056904.1" /locus_tag="SSOG_RS02805" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00579" /product="discoidin domain-containing protein" /protein_id="WP_039940546.1" /transl_table=11 /translation="MGRSSRRLGAWLAAVLLAIGTLALAGPVPARAEVVNPRQQWLRDS QAGLFLHWGMRTSPGYTSCAAWEKAVTDGGWSAGYWVDEAKKLHAKYLVLASFHSRLGY ARAWPSAIPGSCSTKRDFLGELVDAAGKQGLKVMLYMTDDPQWHDEGGHEWLDSAAYSA SKGRDVDLTTRDGFGEYSYDNFVEVMKKYPKLSGFWIDNDNDYWLDHGLYEKIRADRPS WLLSNNNEDTPIMDTVSNEQKTGMTPAYDYPQAIWTPQPRLTEACFKLPSSGAWWYSGT DSAVDHGLTLGRLISNSGSSVKSLMAETAQVNGKFPSKQEAFNNAAKTYLDKIWGSLDG TEGGGYAYGGLQPGSWNDGARGVTTVSRTDPDQHYLHVLTKPSGSTLTVRDNGYRVSGV TDFRTGRKLAFSQGEGKLTVSGISGWDPYDTVLSVRTDGRQGLIPADSMTASASASASG HPASAVLDGDPQTYWDNNTKLPATVTFDLRSARSVRYLAVNQREWSVSYARSDTEQSAR IRDYQVQVSGDGSTWGSPVKAGTLPSARGVQFIDLPATTKRFVRLTVNSTWAAATDTKR YQKLLIDEVRIGSGYPKGDS" gene 16763..17569 /locus_tag="SSOG_RS02810" /old_locus_tag="SSOG_00580" CDS 16763..17569 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014056901.1" /locus_tag="SSOG_RS02810" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00580" /product="hypothetical protein" /protein_id="WP_039936412.1" /transl_table=11 /translation="MERPPLLPRTALTLAATAALIAPAAVAYGTERSPQAPAASWSLKW NPASGASVASAFEGAEDDRADSHPGTTHIYPSQGGFRTDMHYPRDVDTSTDRQRNEVRG MKQDGSAVKILKNETWRIQYQMYVPDTLDATTSFTHIMQIKVGDVAAPLFTMSLHQHDN GPKVEIRTSDDDDNLTEAGAANMAPLQGAWSDVSVEVKAADSGAYADWSVTTGGKEVAS YKRTGLDLWRGKNYLRPKWGIYRSLNSSGLQTTYLLTRNFKAYKLQ" misc_feature 16781..16783 /note="tta leucine codon, possible target for bldA regulation" /tool="antismash" gene 17581..18663 /locus_tag="SSOG_RS02815" /old_locus_tag="SSOG_00581" CDS 17581..18663 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009712691.1" /locus_tag="SSOG_RS02815" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00581" /product="PQQ-dependent sugar dehydrogenase" /protein_id="WP_009712691.1" /transl_table=11 /translation="MKSCRNTLVAAAATTALTAGLLLTQAGTGNAAPAAADSVPSAIST VSSGWSIPWGLSWLPDGSALISERDSFKLFKLTQSGAKTQVGTVPNVVTTSGEGGLMGL AVSPNWNTDHYVYIMHTASDGNRIARMTYDGSSLSGYKAIVTGIKKNKYHNGGRLKFGP DGYLYATTGDAQTSSNAQDKNSLNGKILRMTTDGKAAPGNPFGTLVYSLGHRNPQGLTW DAQGRLWEAELGNSTYDELNLIEAGKNYGWPTCEGSCSTSGMTNPKRQWTVGEASPSGV TYADGALYMAALRGERLWRIPVDGTSAGTPKAYYTSSYGRLRTVETVPGKNTLWLSTTN ADNNGGEADGADKVFQVELK" gene complement(18720..19871) /locus_tag="SSOG_RS02820" /old_locus_tag="SSOG_00582" CDS complement(18720..19871) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014174701.1" /locus_tag="SSOG_RS02820" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00582" /product="glycoside hydrolase family 88 protein" /protein_id="WP_009712692.1" /transl_table=11 /translation="MIRRRTVLASALGIGGGLLLPGTGVAHAAARTDWSRAVVDSTVAR YPDPASLGGWGYTRGLFLLGAYRVYQRVGEPSYLAYIKRWVDNFVDADGHMSRTFDNLD AMQSGNLLLMLHRETGDARYRTAADQIRARFADYPRTADGGMWHATGKTNELWGDGVFM AQPFLLRYGIAYGDKSYAYDEVTRNLAVYFRHLKASNGLIYHAYDADGDAPWNPDDATG TSAHFWARAIGWTAMTHIEVLEMLPARHPRRAELIANVRHLAKGFLRYQDRATGRWFQV VDKPGEPGNWTETSASSMYTLMLHAALDHGWIRGGDYAAAARRGYRGVLDKVTVDADGL TSIADISEGTNVGDLAYYLGRKRNTNDLHGLGAFLLMNERLAY" gene complement(19888..20925) /locus_tag="SSOG_RS02825" /old_locus_tag="SSOG_00583" CDS complement(19888..20925) /EC_number="1.-.-.-" /GO_function="GO:0016491 - oxidoreductase activity [Evidence IEA]" /GO_function="GO:0071949 - FAD binding [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) DAO" /gene_functions="biosynthetic-additional (smcogs) SMCOG1103: FAD dependent oxidoreductase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: protein motif:HMM:NF013434.4" /locus_tag="SSOG_RS02825" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="SSOG_00583" /product="FAD-dependent oxidoreductase" /protein_id="WP_078510228.1" /sec_met_domain="DAO (E-value: 1.5e-45, bitscore: 155.4, seeds: 521, tool: rule-based-clusters)" /transl_table=11 /translation="MRRPAPNWCASSSASGCAPPHATTTAARARSARSSKGTFELFEEL EALGVDGQAHKDGFLFAFPSRAYAAKALTAFRRLGAPIAAGGVLTGDALADTEPALTEG ARAGFVVDDQWSLDPGLFVDRLAERLRRQGVELVEGARVSAVSERAGRAEVRTSAGTYR SDAVVVAAGIWSRELVRGLGVDLDLVAGKGYSFSVAAEPPPSRLVHLGAAKVVLTPMGK RVRVAGTMEFERDPDRFRRRRVEAIVAAARPYLRHADWDDLQEEWVGPRPMTPDGLPLI GRVPGHSRVLLATGHNMLGLMLAPATGRLVAGLLTGDADPALSSRFDPVRCARRYPWRV HGILN" misc_feature 21586..21595 /note="TFBS match to AbrC3, Antibiotic production activator, confidence: strong, score: 18.07" /tool="antismash" misc_feature 21732..21746 /note="TFBS match to VqsM, Regulator of quorum-sensing signalling systems, confidence: medium, score: 17.83" /tool="antismash" ORIGIN 1 ggatggacgc ccgggtacgc gacggcggcc acgggcccgc cgagctgcgg ctgctggaac 61 ggcggttcaa cgccatggcc gacgcggtct ccaccgccct ggagcggcag cgcgccttcg 121 tcgcggacgc ctcgcacgaa ctgcgcaccc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn 181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccccgccgct gcccgcaccg 481 gccgcccgga tcgcggacat cgcgctcgac aactcggcca agttcgtgcc gtccggcggc 541 acggtcaccg tctcgctcga cgccgccgga gcgctgcgga tcgccgacga cggccccggg 601 ctcgcccccg aacagctggc cgcggccccc ggccgcttct ggcgcgcccc ggaccacggg 661 aacgtccccg gcagcggcct gggcctggcc atcgccgccg aactggcccg ctcctgcggc 721 gccgagctga ccctgcgctc cgccgagcca cgcggcctgg tggtggagct gcgcctgccg 781 gtgggctagg gcgggttcct ctcccccacc ccgccgcttc ccgaaacggg ggacaccccc 841 gcccccggga ggtccacccc gacggtgcgg aaggcgttgg cgcgggcagg gcgcgcgcac 901 tgcggggccg ccgcgtaccc gccggggagg cgagccgctg cctccacgcc ccttcgcgca 961 cgccggatgc gcaccgcggc agcggcagcg ggcagcggca gcggcagcgg cagcggcagc 1021 ggcagcggca ggcgaacgtg cagcccaggg gctccgccgt gcgggccacc gcgctccgcg 1081 gcacccggcg cgcccggcgc cgcttcggca gcggggtcgg agcccgcggc gggccggggc 1141 gtcagccgta ggcggcgcgg tagtaggcgg cggcgcccgg gtggaggggg acggtgccgg 1201 tgccgatggc gtaccgctcg tccaggcggc tgcccggcgc gtccggcacc ggcagccggt 1261 cgcggcgctc gaagagcaca tcggtgagcg cgcgggccgt accgtcgggg agcccggagc 1321 ggcagacgag atagctgggc gtgcccacgg tgggggtcgg ccccttcagc ccgtacgccc 1381 cggccgggac ggagacctcc tcgtagaccg ggccgtggtc gcggcgcagc gcgcgggcga 1441 gcccgccgag ggggatcagg cggagcgggc ggcggcgggc gagccgggag atggcccagg 1501 tgggggcgcc gccggaccag aagaaggcgt ccacttcccc gtcggtgagc gcgtcggcgg 1561 aggcgccgag cccgaggcgt acggcgcgga cgtcgcgtac cgcggcggcg gcgagcaccc 1621 gtccggcggt cgtcgaggtt ccggacccgg cggcgccgag ggagaccgtg cgcccggcca 1681 gctgggcggg gtgctcgatc gtggagtcgg cgggcacgac caggtgcaga tagttgaggt 1741 acagccgggc gagggccgcg accgggatct tcctcgggaa gatgccgtgc cccgcggcgg 1801 cgtccgccgc gctgtcggcg agggtgagcg cgaggtcggc ccggccgtcg ccgagcatgc 1861 gcaggttctc cacgctcgcc gcggtggtga gcacccgcac ggtgagcccg tggtgggcgc 1921 ggtgcgcctc ggtggccagt cggctgccga aggtggcgta ggggccaccc cgcgggccgg 1981 tggccagccg cacctgggcg gagggcccgg ggtccgaaca accggcgagc ccgcccgcgg 2041 cgagcgccag cagcaggcgg cgtgggacgg tgtgcacccg gcgatgctaa ccgccggggt 2101 ccgtactggt cagcgggcca gcgcgccgag ctgggcccac accacgcggc cgcccgccgc 2161 ccccggccgg tcgccccagg cctcggtcag ggcgcggacg agggccagac cgcggccgct 2221 ctcgtcctcc acacccgcct cccggggcat ggggccgagc gggccgccgc cctggtcggc 2281 gacctcgacg tacagcgagc gccgctcctg ggcgcggagc acgcagagga tctggtcgct 2341 cgcggtgtgc acgagcgcgt tcgtgaacag ctcggacatc accagctcgg ccgcgccgca 2401 cacgtcctcg tcgaagcccc agtggggcac ccggttccgg acgagccgtc gcgcggcggc 2461 gaccgaggca ccccgggcgg gcagttgcag actctcctgg cggagggcca tatcaacgcc 2521 ggcggagctc atgtgagggg ccatcgtcga gcgttgacgc aatgccacgg ccatgtgcct 2581 tccgtccgtc tacctgtggg ggcgtgaacg ccgcccgtac gcgactgccg caagtgcgcg 2641 acagggcagc cacgttggga ccggtcacga cgccactatc gttcctgcga gcatcagttg 2701 gcaaggcgcg ttctgcaaaa tgcagaatgt ccacggcagt ctgtgccgct cacaccgagc 2761 atggcacact gagacgcaca acaccgtcgg aggaggtatg acgtgggtga agcgcggtct 2821 gccccgaccg tcgggcagat ggtcctcggc atgcggctgc ggcacctccg cgagcaggcc 2881 ggcgtcggtt atgacgcggc cgccaaggcg ctgcatgtca accagaccac cgtgcgccgg 2941 atggagaagg ccgaggtcgg cctgaagctc ccgtacgtcg agaagctcct gcggacgtac 3001 ggcgtcggac aggacgaggt ggagtccttc ctcgggctcg tcgaggaggc caacaggcct 3061 ggctggtggc accggttccg cgatgtgctg cccgactggt tcagcctcta tgtgagcctg 3121 gagggcgccg ccagccgcat ccgcgcgtac gaaccgcact tcatccccgg tctgctccag 3181 acggaggact acgcccgcgc gctgctgcgc atcggcttcc cgcagggctc cgaggaggag 3241 ctgggccggc gggtcgccct gcggatggag cgtcaggagc tgctctccag acccggcggg 3301 ccgcatctgt gggccgtcat ggacgagacg gtgctccgtc tgcacatcgg cgggccgaag 3361 atcatgcgcg cccagatcga ccgcctcatc gaggccatcc agatgccgaa cgtgaccctc 3421 caggtggtgc cgttcgccgc cgggccgcat ccgggcatgg gtggcccctt ccagctgttc 3481 cggttccaga tcccggaact gccggacatc gtctacgccg agggcctgac cggcgccggc 3541 tatctcgacc agcgcaccga tgtggccgcc tatctggagg cactggacca gatgggcacc 3601 cgggccacat cggcgcggcg cacggagggc tttctccgag agattcgcaa ggagctgtga 3661 ctgtggatca ggtagaagac gtctacaacg gcatgcccgc cacccgtctg gcgacggaag 3721 gctggcgcaa gccctggagc ggccccaacg gcggtgcgtg cgtggaggcc ctgaggctgc 3781 acgacgggcg gatcgccctg cggcagtcga cggaccccga cggccccgcg ctgatctaca 3841 cccaccatga gatggagaag ttcatccagg gcgccaaggc cggtgccgcg gatttccttc 3901 tcacccgacc cgacaacaac aacctggcca cctcggcggc taacgcaccg gaacggaggg 3961 cggcgtgacc gactacggac tcgccccgga ccagcagatc gacaccagca agccgcactc 4021 ggctcggatg tacgacttct acctcaacgg caaggaccac tacgaggtcg acgcggaagc 4081 cgcgggcaag gtgctgtcgg tctacccgac cgtcaagctc aacgccaccg tcaaccgcgg 4141 cttcatccac cgggcgacgc gctggctggc ggccgaggcg ggcatccggc agttcctgga 4201 catcggcacc ggcatcccca ccgagccgaa tctgcaccag gtcgcccaga gcgtggcccc 4261 ggaggcccgg gtcgtctaca ccgactacga ccccatcgtg ctcgcgtacg cccaggccct 4321 gctgcgcggc acccccgagg gccgcaccgc gtacgtccag gccgatgtgc gcgaaccgca 4381 gcggatcatc tcggcgaccg agctgcggga gacgctggac ctgagcaagc cggtcgcgat 4441 gtcggtcaac gcgctcttcc acttcattcc ggaggaggag aagccgtacg agctggtgcg 4501 tgagctgatg ggcgcgctcg ccccgggcag ctatctgatg ctgacgcact ccacggccga 4561 cttccacgcg gagacgtggc agaaggtgat cgccgtctac aaggagagcg gcatatcctt 4621 cgtcacccgc agcaagagcg aggtcgcggc gttcttcgac ggcatggagc tggtcgaccc 4681 gggcgtctcg gagccgcacg actggaagcc ggaccccggc gaccgcccgg acgagatccg 4741 cagcgcggac atcaacatgt gggccggagt ggcccgcaag ccgtagccga agaagactac 4801 ggctcctcgg ggccgtccac ggtgaagccg aggaccgcgc cgccatcggg gcgggggcgg 4861 gcgaagaccg tgccgccgtg cgcgagggcc acctcgcgca cgatggcaag acccagcccg 4921 gaaccgggca ggctgcgggc gccgggggcg cggtagaagc ggtcgaagac ccgctcgcgg 4981 tcctcctccg cgatgccggg gccccggtcg cgtacctcga cgcggtgccc gctcaccacg 5041 acctcgacgg gcccggtgcc gtcccggtcg aacttggcgg cgttctccac caggttggag 5101 accgcgcgca gcagggcggc cggatggccc tcgacgaggg tgtcgccctt ggcccgcacc 5161 gtgatgtcgc ggccggtgcg ccggcgggcg agggtcgcgg ccttggtggc gacctcgtcc 5221 agggacacct ccgtgcgcgg ttcgtcgtcc cgctgaccgg ccgccaggtc caccagctca 5281 ttgacgaggt cggtcagttc ccgggtctcc cccgccagat cggccagcag ctcgtcgcgc 5341 gcggcgggcg gcagcttctc gaagcggctc agcagcgaga tgttggtgcg cagcgaggtg 5401 agcggggtgc gcagttcgtg tccggcgtcc tggacgaggc gccgctggtc gtccttggag 5461 cgggcgagcc gcccgagcat gccgtcgaac gcccggccca gccgtcccac ttcgtcgtcc 5521 ccggcgaccg ggaccgccac ctccatccgg ccggtgaccg ccacgctctc ggcgacccgg 5581 gtgagccggg tgagccgccg ggtgatgcgt ccggcgagcc accacccggc cagtccggcg 5641 gccacgatga tcgcgacgac gaacagcgcg gtgcgcttct ggagttcgcg cagcagatcc 5701 tcggtgtcgc tgatctgctg ggccacctgt acggcgccgc ggtggtcgcc gagcgcgacg 5761 gtggccatcc ggtagtcgtc ctcgccgatg gagatgttgc gctgccgcat ggtgcccggg 5821 gcggcggccg cggcggtctc ccggtcctcg tcgccgaccg gcagcgcggg cttgccctcg 5881 tcgtagacgc cgccccggcc gcccagcacc tggacgtcca tgcggctgat ccggcgcagg 5941 tcgtcgcgca gcccgtaggg caccgggacc tcggaggggt actgccgcag gatcactttc 6001 tggccacgca cctgggtccg cagatcgcgg atgacgtcgg tgaacgcgga cttctggtcg 6061 acccgcacca gcccggccgc ggcgtcgtag ctgaggaagc ccaccaggag ggtcaccacg 6121 gacgccaccg ccgcgaagga cagcgcgaag gtggtgcgca ggctgggccg tggatgacgg 6181 cggagtggga agcgcaccgt tcactcctcc cgcagggtgt agccgacgcc gcgcaccgtg 6241 tggatgagcg gggtgagccc ggtctggtcg accttgcggc gcaaataacc gatgtatacg 6301 gccaggttct tcgatccggg gccgaagtca tagccccaga tgcgagagta gatcgtgcca 6361 tgatcaagaa cgatgcccgc gttacgggcc agcagctcca gcaggtcgaa ttcggtgcgg 6421 gtcagctcca gttcggtacc tccccgccac acccggcgcg ccgagggatc gagccgcaga 6481 tcggcgaccg ccagcgtggc gtcgaagacg gtgccctccg tcgtgggctc cggtacgggg 6541 gcgacgcggc gcagcagcgc gcgcagccgg gcgaagacct cctcgacgtc gaacggcttg 6601 gccacatagt cgtcggcgcc cgcgtccagt ccctcgatcc ggtcggcggt ctccacccgc 6661 gcggtgagca tgaggatggg ggtgcggtcg ccctcggcgc gcagcacccg gcacacctgg 6721 attccgtcca ctcccggcat catcacgtcc agcacgatca cgtccgggtg ctcacggtgg 6781 atggccgcca gggcctcgac gccgtcggac accgaggtca cccggtatcc ctccagcgtc 6841 agggcgcgct ccagcgcatg gcggatggca cggtcgtcct cggcgagcag cacggtgttg 6901 gtcacaggac gagtctgcca aggcccgtcg gatgcggtcc cccgcagccc cccggagctg 6961 cggttcttac ccggctctca ccgtatccac gcgaccggct taccgagccg gtccagggta 7021 cccagtacgt tccgggggga tcgcgccccg gtgttgccac cggccggaga gacaccgaat 7081 gaagatcgta ttcctgctgc acaacgctta tgggatcggc ggaacgatac gcaccacgct 7141 gaacctcgcc gcggcgctgg cggagcggca cgaggtggag atcgtctcga tgatgcgcca 7201 ccgggggaag ccgcgcttca cgctcgaccc gcgggtgcgg ctggtgccgc tggtcgacac 7261 ccggcccggc ggcctggacg cgcgggatcc gctgttcgcg gagcgcaccc gggactttcc 7321 cgtccacgag aagcggaacc accagtacaa ccggctgatg gacgtgcgga tcgcccgcta 7381 cctggagggc tccgacgccg acgtgatcat cggcacccgg ccgggcgtca atgtgtacct 7441 cgcccgcttc ggcccgcgca aggccctgcg catcgcgcag gagcatctgc ggtacgaggc 7501 gcacagcaag aagctgcggg ccgagctggc gccgtactac cgcgagctgg acgccgtcgt 7561 caccacgacg gaggcggacg cggcggtgta ccgggcgaag atgccgctgc cgggggcgtg 7621 gaccctcgcg ataccgaaca gcgttcccga gcccgccgcc ccgccctccg agggcaccac 7681 gccggtgatc gcggcggccg ggcggctggt ccgcggcaag cgcttcgacc tgctgataga 7741 cgccttcgcc gaggtctcgc ccaagcatcc ggagtggcgg ctgcggatct acggcggtgg 7801 ttcccagaag gaccggctcc aggagcgcat cgaggagctg gggctgaccg cgagcgccga 7861 gctgatgggg ccgtactcgc cgatcgagga ggagttcgcg aaggcgtcga tcgtcgcggt 7921 cgcctcggac gccgagtcgt tcgggatgac gatcgtggag gccatgcgct gcggggtgcc 7981 ggtggtgagc acggactgcc cgctgggccc ggccgagatc atcgaggacg gggtcacggg 8041 tcgcctcata ccggtcggcg acaagcaggc gatggcgcag gcgctgcggg atctgatcga 8101 tgacgagccg gggcgccgcg cgatggccgc ggcggcgctg gagagcgccc ggatgtacga 8161 cccggagccg atcgccatgc gctacgagca gctcttcgag gagctggcgt cgagccggtt 8221 cgcgcgcaaa tgggagcgca cccgggtccg gctgcgggcg agctaccgcc gtaaggtccg 8281 ccgctggaag cgcgccttcc gccgccgtct gcccgcggcg ggcgggacga gcgccaaggc 8341 gtgacgcccc ctgggccggg cggtgtccgc cgcccggcct gcccgccgga ttaccaggtg 8401 gtcggcgcgt agtccttcag gaagcagccg aacaggtcct cgccctgctc accccggacg 8461 atcgggtcgt agacccgggc cgcgccgtcc accaggtcca gcggggcgtg gaagccgtcg 8521 gccgcgagcc gcatcttgtc cgggtgcggg cgctcgtcgg tgatccatcc ggtgtcgacg 8581 gccgtcatga ggatggcgtc gctctcgaac atctcacggg cgctggtgcg ggtgagcatg 8641 ttgagcgcgg ccttggccat gttggtgtgc ggatggcccg cgcccttgta gccgcggccg 8701 aatttgccct ccatcgcgga gacgttgacc acgtacttcc gccgggcggg cgcggcggcc 8761 atcgccgggc gcagccggct gaccaggatg aagggcgccg tctcgttgca cagctgcacc 8821 tccagcatct cgaccgggtc cacctcgtgg acccgctgga tccaggtgtt ggtggggtcc 8881 aggtccggga cgagcccgcc cgcgtcgatc gcggtgcccg cctcgatccg ggcgggcgag 8941 gcggagccgc tggtgagggc gagggcggtg aggtcctggg cggtcagcgc gccgtcccgc 9001 tcccgtcgcg gcccgggcag cgcggtctgg gagccgctgc cgaaggcgcc gaggacgacg 9061 gcctcgggca gctccccggc gggcagcggc gccgactcgc ccgctatcag ctccccgtac 9121 gcctgcgggc tgcggcgcac ggtctgcgcg gcgttgttga tcaggatgtc cagcggcccg 9181 gcggcgctga cctcgtcggt cagcgcgatg acctgggcgg ggtcgcgcag atcgatgccg 9241 acgatcttca gccggtggag ccactcggcg ctgtccggca tcgccgtgaa gcggcggatc 9301 gcgtcgttgg ggaaacgggt ggtgacggtg gtgtgggcgc cgtcacgcag tagccgcagg 9361 gcgatgtaca tgccgatctt ggcgcggccg ccggtgagca gcgcccggcg gccggtgagg 9421 tcggtgcggg cgtcgcggcg ggcccggttc tcggcggcgc agccgcggca gagctggtgg 9481 tagaacgcgt cgacctcgac gtaccgggtc ttgcacacgt agcaggagcg cgggcgctcc 9541 aggatgcccg cgatctcgct ggtgaccgag gagctgaggg cccggcccag cgtctcgtcg 9601 tcgatgcggt cggcggcgcc ggtggcggtg gcctcggtca ccgccttgtc gttggcggtc 9661 ttggcggccc ggcgttccga gcgcctgcgc tgcttcacgg tgcggtagat accggcggtg 9721 gcccggcgca ccgtgatggc gtcggggtgg tcgatgggca gccggtccag ctcctcgagg 9781 acgctcaggc aggtcgccag gcgctcgggg tcgataccgg gctcgtcggt accgggcgcc 9841 tcggcccccg ggtactcggc ccccggccgc ccggcctcca tccgctgggc ccggccgtcg 9901 tcagtcaccg tcatcgccgc tgccgctttc tggttctgtt ctccctggtt aacggccgaa 9961 ctttacggaa gcggggacgg ttgagccaaa ccgggcggcc tcaccccctc ccgtacccgc 10021 ggtacgtata gcccggaggc gggggctccg cggccggagc ggggccggtc agcgaacgga 10081 tcaggccatg atcgggcgag agggcgcagg ccgggtcggt gcgggcggcg tccccggtga 10141 gggcgtacgc ctggcagcgg cagccgccga agtcctcggc gcggtgggag cagctacgac 10201 acggttcggc catccactcc tcgccgcggt agcggttgaa ggcggcggag tggtcccaga 10261 tccactccag ggcgtggtcg cggacgttcg gcgggtcgag gccgggcagg gcggccgcgg 10321 cggggcaggg caggacggtg ccgtcgggcg cgacggtgag cgagaccgca ccccagccgc 10381 ccatgcaggg cttggcgacg ccctcgaagt agtccgggag gacccacacc agctcggtgc 10441 ggccgccgag cttctcgcgc catcgcgcga cgccctcccg ggcccgggcg agctgctcgc 10501 gggtgggcag cagggcggcg cggttgcgca gggcccagcc gtagaactgg gtgttggcga 10561 gttcgacgcg atcggcgccc caggccacgg ccagttcgat cagtgggtcc agcgcgtcga 10621 ggttgtcgcg gtgcaggacg acgttgacgc ccagcggcag gcccgcctcc cgtaccaggg 10681 cggcggcccg ctccttggcg gcgaacgagc ggtgtccggc gatccggtcg gaggccccgg 10741 ggtcggcgtg ctggacggag agctggacgc tgtgcagccc ggccccggtc agcgccgtca 10801 gccgggcctc gtcaagaccc acgccgctgg tgaccagctg ggtgtagagc ccggcggacg 10861 cggccgcggc gacgatcacc tccaggtccc ggcgcagcag cggctcaccg ccggacagat 10921 gggtgtggac gacgcccagc tccgccgcct ggcgcagcac ccgctcccac tcctcgccgg 10981 tcagctcgcg ggagcggcgc gtcagttgga gcgggttgga gcagtagggg cagtgcagcg 11041 ggcagccgtg ggtcagctcg gcgagcagcg cccacggacg ggggtgccct tgggggtggg 11101 cggtcaccgt atccagccct cctcgtgcat ccggtccagg aacgccggga cgtcggtggc 11161 cacgggggcg ccggggaagc gttcggcgag ttcggccacg atggtgtcga ggtcgcggcc 11221 gccgtcgcac agccgcagca ccgttccggc ggtgccgcgc aggaccacca cgcgctcggg 11281 catcaccagc aggtccacct cgcgcacccg gtcgtggcgc aggtgcaccg cgggggccag 11341 ggccggccgc caggcggccg gggccgtcat atcccctccc ccgccgtgtc cttggtgtcg 11401 gcgtggtcca cggcgtccag cagggaccac agcacatcgc atttgaaggt cagcgcagcg 11461 accgcccgtt cctcgtcctc gggggtgcag gcccaggtca ggacgaggtc gagggcttcg 11521 gcgctgtccc ggctgccctg acccacccgg tcccggaagt agcggagccc ttcgcgctcg 11581 atccacgggt agtggtgttc gaacgcctcg atgcgggtgc gcatgatgcc gggggcggac 11641 agttcggtca gcgaggcggc gacggcctcc agcggggtgc ccagacggca gaagttgacg 11701 taaccgtcga ccgcgagccg tacgccgggc agcacttcgg cgccggtgag cagccgttcc 11761 cggccgatgc ccgcggcctc gccgagccgc agccaccgct cgatgccgcc ctcgccgtcg 11821 gcggcgccgt cgtggtcctg gatgcggcgc agccacatcc ggcgcagccg cggggtgtcc 11881 agcttggcgg tgatgagcgc gtccttgacg gggatgtggc gctggtagtg gaagcggttg 11941 aggatccagc ggcgcagttc ggcgggggtg agttcgcccg cgtgcatgcg gaggttgaag 12001 gggtgccggt cgtggtagcg ctcctgggcg actgcgcgca gccgctcttc gaggctccgg 12061 gtcataactc gatcaccatc ccgtcggcgg ccacctcgat gccccgtccg gcgagcaccg 12121 cgtgctgggg cgcggccggg tcgttgaggg ggttggtgtt gttgaggtgg gtgtacaggg 12181 tgcgggcgcc gagtgcggcg agccgccccg ccgttccgcc gggtccgtcg atcggcacat 12241 ggcccatggc ggtggcggta cgggagccga agccgctgcg caggggctcc tcgtcgtccc 12301 agaaggtgcc gtcgacgatg acgcagtcgg cctcctcggc ggcccgctgg aaggcgtcgg 12361 accaggcggc gagggcgggg gcgtagagga gggagcggcc ggtggcgcgg tcccgcaggt 12421 gcagggcgac gacccagtcg tcctcgtcgg gaccgtccaa ctcggcggca tagcgggggc 12481 gtttggcgga gacggggacg gcggcgatgg cgaagtcggc atcgcnnnnn nnnnnnnnnn 12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12841 nnnnnnnnnn nnnnnnnnnn nnngggtggg cgcgtgcccc cggagcatcc ggggcacgcg 12901 cagttccact ggggtacgcc gccgcccgcc gcggtgccga gcacacgcac gcgcatggcg 12961 gctgccgtca gcgggcgttg agcgagtagg cggtgacctc cagcgcggtc tcgacgacca 13021 cgtagtcggg ggtctgccag gctgtttccg gcgcgggggt ccgggtgtgg tccaccgcct 13081 cgggagccgt ctgccgcacg gtttcgttca tggcattgac ctgccttccg gtttccgggt 13141 gtcgggttgg gccagagggt gtctcggcgg gacgtcagcc ggtggtcctg atccgcagcg 13201 cctcgccgtc ggtggtggtg ctgtgccgga ccacggggcc ggtgacgccg ctgaagccca 13261 cgtcgtacgg actggcggtg ccgcgcccgg ccgtgacccg gtcgagggtg atgcctaccc 13321 cgtcgtcggc gatgaacgcg ggccggtcgt cgttggtgtc ggtcgtgacg gcgctgttgg 13381 tgaaggtgat gcccttggcg tgccggatcc accagccgta ggacgggcgc accccatagg 13441 tcttggggac gtagtcggtg aggaactcgc cggggaccag ctccgcgtcg gaggcggggt 13501 ggccgccggg gacggtcagc cgcacccggt cgaaggtgat gtcctccacg tccgcgccgg 13561 gcgctccggt gatggtgctg gtgtagtcga cgccggtgac cgggtgggtc agcccggtgc 13621 cggtgacgtt cctgaagcgg atgtcgtgga tccggcccgc gggcggggcg cccgggcagc 13681 gctttcggtc gccgatcttg atgaagacgg gggtggcgcc cttggtcatg gtgatgtccg 13741 agtagcggac gtcgctgatg tcggcgccgt ccatggagac gatgccgatg gccgccttgc 13801 ccccgccgcc gatccggagc cggtcgaagc ggatgtcgcg gaagtcgcca caggtctccg 13861 agccgaactg gagcgcgttg ttctcggtgg acagcacatc ggagtcatgg acccggacgt 13921 cctggctggt gtaggtccgg cccagcgcgt agtcgctctt gaggacgacg ccgtcgtccc 13981 ggccctcgat gcgggactgg ccgatgtcga catggcggct gttgatgagg ttgacggcgt 14041 cccggtcggt gctggtgagc accttggtgc gggtcatccg tacgccgtcg cagccgttga 14101 gcagcagcgc gaagtggccg ccccgggtga aggtgacgtc ctggatgtcc aggtcggcgc 14161 agcgcttgag ggagaggatc ttgtcgccga cgcccggttc cagggtgttg ctggtgccca 14221 gcccctcccc gtcgagggtg ccggacccgg tgatggcgag gtggtggatg ttctcgcccg 14281 tcagcagcgc gttgcggaag tggctgtggc cgaagtcctg gtaggcgtcc caggggttgg 14341 gctcggcctc gtccatgcct ccggcggcgg cgagcagcgt cgcgcccttg gcgaggtgca 14401 gggtgatgtc gctcttgagg tggagggtgc gggacttgta ggtgccgggc gggaacagca 14461 cggtgccgcg gccgggagcg gcggctgccg cgtcgatcgc ccggtcgatg gcgtcgtcgt 14521 ccagcgtcgt gccgtcgccc gtggccccgt agtcgcgtac gtcgtacgta cggtcggcgg 14581 cgccgggctg tgcggtggcg gtctggggag ccacggcgag caggcaggct gcggccgccg 14641 tgatgacggc ggtccgtaag cgcgtcgagc gcgtcatgaa tcgcccttcg ggtagccgga 14701 tccgatgcgc acctcgtcga tcagcagctt ctgatagcgc ttggtgtcgg tggccgcggc 14761 ccaggtgctg ttgacggtga gccggacaaa gcgcttggtg gtcgcgggga ggtcgatgaa 14821 ctgcaccccg cgggcgctgg gcagggttcc ggccttgacc gggctgcccc aggtgctgcc 14881 gtcgccggag acctgcacct ggtagtcacg gatccgggcg gactgttcgg tgtcggaccg 14941 ggcgtaggag accgaccact cccgctggtt gacggccaga taccggacgc ttctggccga 15001 gcgcagatcg aaggtgaccg tggcgggcag cttggtgttg ttgtcccagt aggtctgcgg 15061 atcgccgtcg aggacggcgg aggccgggtg gccgctcgcc gaggcactgg cgctcgcggt 15121 catggagtcg gccggaatca gcccctgcct gccgtcggtc cggacgctca ggacggtgtc 15181 gtacggatcc cagccgctga tgccggagac ggtgagcttt ccctcgccct ggctgaaggc 15241 cagtttcctt ccggtacgga agtcggtgac gcccgatacc cggtagccgt tgtcccgtac 15301 ggtcagtgtg gagccggacg gcttggtcag gacgtggagg tagtgctggt cggggtcggt 15361 cctgctgacc gtggtcaccc cgcgggcgcc gtcgttccag gagccgggct ggagcccgcc 15421 gtacgcatag ccaccgccct cggtgccgtc cagcgagccc cagatcttgt cgagataggt 15481 cttcgcggcg ttgttgaagg cttcctgttt gctggggaac ttcccgttca cctgggctgt 15541 ttcggccatg agcgacttca cggaggatcc ggagttgctg atcagccggc ccagggtgag 15601 tccgtggtcc accgccgagt cggtgccgct gtaccaccag gcgccgctgc tcgggagctt 15661 gaagcaggcc tcggtgagcc gcggctgagg ggtccagatg gcctgcggat agtcgtacgc 15721 cggggtcatg ccggtcttct gctcattgct gaccgtgtcc atgatcggcg tgtcttcgtt 15781 gttgttgctc agcagccagg acgggcggtc ggcgcggatc ttctcgtaga ggccgtggtc 15841 gagccagtag tcgttgtcgt tgtcgatcca gaaacccgaa agcttcgggt acttcttcat 15901 cacttcgacg aagttgtcgt acgagtactc accgaatccg tcgcgcgtgg tcaggtcgac 15961 gtcacggccc ttggaggcgg agtacgccgc cgagtcgagc cattcatggc cgccctcgtc 16021 gtgccactgc ggatcgtcgg tcatgtagag catgaccttc agcccctgct tgcccgccgc 16081 gtcgaccagc tcgcccagga agtcccgctt ggtggagcaa ctgcccggga tggcggaggg 16141 ccaggcgcgc gcgtatccca gccggctgtg gaaggaggcg aggacgaggt acttggcgtg 16201 cagcttcttg gcctcgtcca cccagtagcc ggcactccag ccgccgtcgg tcaccgcctt 16261 ctcccaggcg gcgcagctgg tgtagccggg cgaggtgcgc atgccccagt gcaggaagag 16321 gcccgcctgg gagtcccgga gccactgttg ccgggggttg accacctcgg cccgggcggg 16381 gaccggaccg gccagggcga gcgtgccgat ggccagcagg acggcggcca gccaggcgcc 16441 taggcggcgc gaactgcggc ccatgggcgg ctccttctcg ggggcgcggt ggggtgcgac 16501 gggatggcgt tcctgatgca gcgtcacggc ctggcggtgt ccggtcaata agcgtgcagt 16561 caaaatcctc cgatctcttg tgcgggacat acggtgcacg attcagcgaa acaagcggaa 16621 tccgcaggta gttgaggggt tcaggccccg agaacctgcg gcgaccgccc tcttggtccg 16681 caccattcct tgactcatga gggcacacct cctaccttca cagtccccca atgtgaacaa 16741 ggatcagaca ggagaatcct tgatggagcg cccaccgctc ttaccgcgca ccgccctcac 16801 cctcgcggcc accgccgcgc tgatcgctcc ggccgcggtc gcctacggca cggagcgctc 16861 cccgcaggcg ccggccgcgt cctggagcct caagtggaac cccgcgtcgg gcgcgagtgt 16921 cgccagcgcc ttcgagggcg ccgaggacga ccgcgccgac tcccaccccg gaaccaccca 16981 catctacccc tcccagggcg gtttccggac cgatatgcac taccccaggg acgtggacac 17041 cagcaccgac cgccagcgca acgaggtccg gggcatgaag caggacggct ccgccgtcaa 17101 gatcctgaag aacgagacct ggcgcatcca gtaccagatg tacgtgccgg acacgctgga 17161 cgcgacgacg agcttcaccc acatcatgca gatcaaggtg ggggacgtgg ccgccccgct 17221 gttcaccatg tcgctgcacc agcacgacaa cggcccgaag gtcgaaatcc ggacctcgga 17281 cgacgacgac aacctgaccg aggccggggc cgcgaacatg gcgccgctcc agggcgcctg 17341 gagcgatgtg tcggtcgagg tcaaggcggc cgactcgggc gcgtacgccg actggtcggt 17401 gaccaccggc ggcaaggaag tcgcctcgta caagcgcacc ggcctggacc tgtggcgcgg 17461 caagaactac ctgcggccca agtggggcat ctaccggagc ctgaacagca gcggcctcca 17521 gaccacgtat ctgctcaccc ggaacttcaa ggcgtacaag ctccaatagg aggacacgcg 17581 atgaagagct gtcggaacac cctggtggcc gcggccgcca ccacagcgct caccgccggg 17641 ctgctgctca cccaggcggg caccggcaac gccgcaccgg ccgcggccga ttccgtcccc 17701 tcagccatct cgacggtctc gtccggctgg tcgatcccct gggggctgag ctggctgccg 17761 gacggctcgg cgctgatcag cgagcgggac tcgttcaaac tcttcaagct cacccagtcg 17821 ggcgccaaga cccaggtggg cacggtgccc aatgtggtga ccaccagcgg cgagggcggg 17881 ctgatgggcc tcgcggtgtc cccgaactgg aacaccgatc actacgtcta catcatgcac 17941 acggcgtccg acggcaaccg gatcgcccgc atgacctacg acggttcctc actgagcggc 18001 tacaaggcga tcgtcaccgg catcaagaag aacaagtacc acaacggcgg gcgcctcaag 18061 ttcggcccgg acggctatct gtacgccacc accggggacg cgcagacatc cagcaacgcg 18121 caggacaaga actcgctcaa cggcaagatc ctgcggatga ccaccgacgg caaggccgcc 18181 cccggcaacc cgttcggcac cctcgtctac tccctgggcc accgcaaccc gcagggcctc 18241 acctgggacg cgcagggcag gctgtgggag gcggagctgg gcaacagcac gtacgacgaa 18301 ctcaacctca tcgaggcggg caagaactac ggctggccca cctgcgaggg cagctgctcc 18361 acctccggga tgaccaaccc caagcgccag tggaccgtcg gcgaggcatc gcccagcggg 18421 gtcacctatg ccgacggcgc cctctacatg gcggcgctcc ggggcgagcg gctgtggcgg 18481 atcccggtcg acgggacgag cgcgggcacc ccgaaggcgt actacaccag ctcgtacggg 18541 cggctgcgca ccgtcgagac ggtgcccggc aagaacacgc tgtggctgtc cacgacgaac 18601 gccgacaaca acggcgggga ggcggacggc gcggacaagg tgttccaggt cgagctgaag 18661 tagcacgcgc cggcatgcgt acggtcgcgc tccccgggga gcgcgaccgt acgcccgtgt 18721 cagtacgcca gccgctcgtt catcagcagg aaggcgccca gcccgtgcag gtcgttcgtg 18781 ttgcgcttgc ggccgaggta gtacgccaga tcaccgacgt tggtgccctc gctgatgtcg 18841 gcgatgctgg tcagcccgtc cgcgtcgacc gtcaccttgt ccaggacgcc ccggtagccg 18901 cggcgcgccg ccgccgcgta gtcgccgccg cggatccatc cgtggtccag ggcggcgtgg 18961 agcatcaggg tgtacatgct ggaggccgag gtctcggtcc agttgcccgg ctcgccgggc 19021 ttgtcgacga cctggaacca gcggccggtc gcccggtcct ggtagcgcag gaaccccttg 19081 gccaagtgcc gtacgttggc gatgagttcg gcgcggcgcg gatggcgggc gggcagcatc 19141 tccagcacct cgatgtgcgt catcgcggtc cagccgatgg cgcgggccca gaagtgggcg 19201 gaggtgccgg tggcgtcatc cgggttccag ggggcgtcgc cgtccgcgtc gtaggcgtgg 19261 tagatcagcc cgttcgacgc cttgaggtgg cggaagtaca ccgcgaggtt ccgggtgacc 19321 tcgtcgtacg cgtagctctt gtccccgtac gcgatgccgt accgcagcag gaacggctgg 19381 gccatgaaga cgccgtcgcc ccacagttcg ttggtcttgc cggtggcgtg ccacatgccg 19441 ccgtccgcgg tgcggggata gtcggcgaag cgggcgcgga tctggtcggc ggccgtccgg 19501 tagcgtgcgt cgccggtctc ccggtggagc atcagcagca gattgccgga ctgcatggcg 19561 tcgaggttgt cgaaggtgcg gctcatatgg ccgtcggcgt ccacgaagtt gtccacccag 19621 cgcttgatgt acgccaggta cgagggctcg cccacccgct ggtagacgcg gtacgccccc 19681 agcaggaaca gcccccgggt gtagccccag ccgccgagcg aggccgggtc cgggtagcgg 19741 gcgacggtgg agtccaccac cgcccgcgac cagtcggtcc gggctgccgc gtgggccacc 19801 ccggtccccg gcagcagaag gccgccgccg atgccgagcg cgctcgcgag tacggtcctg 19861 cgtctgatca tggggagttc cctcttttca gttcaggatg ccatgtacac gccaaggata 19921 gcgtcgagcg caccgtaccg ggtcaaacct ggaggaaagg gccggatccg catcccccgt 19981 cagcagcccg gccaccagcc ggccggtggc gggggcgagc atcagcccga gcatgttgtg 20041 accggtcgcc agcagcaccc gggagtggcc gggcacccgg ccgatcagcg gcagcccgtc 20101 gggggtcatg ggccgcgggc ccacccactc ctcctggagg tcgtcccagt cggcatggcg 20161 cagatacggg cgcgcggccg cgacgatggc ctcgacgcgc cgccgccgga accggtcggg 20221 gtcgcgctcg aactccatcg tcccggcgac ccggacccgc ttgcccatgg gcgtgagcac 20281 caccttggcc gcgccgaggt ggaccagccg ggagggcggg ggctcggccg ccaccgagaa 20341 gctgtagccc ttgcccgcca ccagatcgag gtccacgccg agcccgcgga ccagttcgcg 20401 ggaccagatg cccgcggcga cgaccaccgc gtcgctccgg tacgtccccg ccgaggtccg 20461 cacctcggcg cgcccggccc gctcactgac ggccgacacc cgggcgccct cgaccagttc 20521 gacgccctga cggcgcagcc gctcggcgag ccggtccacg aagagcccgg ggtccagcga 20581 ccactggtcg tccaccacga accccgcccg ggccccctcg gtgagcgcgg gctcggtgtc 20641 cgcgagggcg tcccccgtga gcacaccacc ggcggcgatc ggcgcgccca gccggcggaa 20701 ggcggtgagc gccttggcgg cgtacgcgcg ggacggaaag gcgaacagga acccgtcctt 20761 gtgcgcctgg ccgtccacgc cgagcgcctc cagctcctcg aagagttcga aggtgccctt 20821 cgaagagcgc gccgagcgcg ctcgcgccgc ggtcgtggtg gcgtgcggtg gcgcgcagcc 20881 cgaagcggac gaggaagcgc accagttcgg cgctggtcga cggatggacg gccagggcgc 20941 tgtccgggcg gtgcagcccg cgcagcgcgg tgccgatgac gccgggggcg gcgagcggtt 21001 ccaccaggtc ggggcagacc tccccggcgt tgccgcggga ggcgccgctg cccagccggt 21061 cgcgttccag gacggtgacc cgcagcccgg cggcggcgag ggagtgggcg cacatcaggc 21121 cgacgactcc gccgccgacg atcgtgaccg aggagggcgt ggcaccggag agggtcatga 21181 cgtctccttc accgtgggcc gcggtgtggt cgggtcggcg gcggtgccgc ggcggaggcg 21241 ggagcggccg cgcggatcgc cgtcgcggtc gaccgcgctc gcgtcggcga gggtcagccc 21301 gctggtctcc ggcgcgagga cgtatgacgc ggcccagccg atgacgcaga tgaccgcgcc 21361 gaggagcatc gccagccggg tgctgtgggc gaggacgacg ggcgctcccc aggtgccgag 21421 tgcggcgccg atccggctga tgccggtggc gaagccgttg gcggtggcgc gcaccccggt 21481 cgggaagagc tcggagggat agatggcctg gaggcactgg ctggagaagc acgccacgcc 21541 gaaggccgcg aagagcacga ccacgacggc cggtgcggga tgggcgaagg cggcgagggc 21601 gagcaggggg atggcggaga ggccgaagct ccacagcagc agggtgcgcc gccccttgtt 21661 gacgaccagg atcccggaga gcgagccgag cacgaagaag atctgcacca cgaccgagcc 21721 gaggtacgag tcgttgccgt cggccagtcc gaaggaggcc aggatggtgg gttcgtacgt 21781 ggtgatggcg tagatgggca gcagctggca cgcccagaag acgctgatga agacggtcct 21841 gcgcagatag gcgccgcgga agatgtcgcc gtagcgggtg cgcgaggcgg tgtccgcctc 21901 cagctcggcc agggtcgcgt ccgggccgag catctgcttc agcacggcct cggcctcgtc 21961 ggtacgcccc ttgctgagca gccagcgcgg ggactcgggg gtgccgaagc gcagcagcag 22021 gatgaggacg gcggggatcg cgctgctcgc gagcatccag cgcca //