LOCUS       NZ_GG770539            10897 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. SPB074 supercont3.1, whole genome shotgun sequence.
ACCESSION   NZ_GG770539
VERSION     NZ_GG770539.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. SPB074
  ORGANISM  Streptomyces sp. SPB074
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     The genome sequence of Streptomyces sp. strain SPB74
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
REFERENCE   4  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Ward,D., Young,S., Jaffe,D., Gnerre,S., Berlin,A.,
            Heiman,D., Hepburn,T., Sykes,S., Alvarado,L., Kodira,C.D.,
            Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S.,
            Walsh,C.T., Lander,E., Galagan,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2008) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Arachne v. April 2007
            Genome Coverage                   :: 12x
            Sequencing Technology             :: ABI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 01:42:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,117
            CDSs (total)                      :: 6,045
            Genes (coding)                    :: 5,425
            CDSs (with protein)               :: 5,425
            Genes (RNA)                       :: 72
            rRNAs                             :: 6, 1 (5S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 620
            CDSs (without protein)            :: 620
            Pseudo Genes (ambiguous residues) :: 0 of 620
            Pseudo Genes (frameshifted)       :: 261 of 620
            Pseudo Genes (incomplete)         :: 378 of 620
            Pseudo Genes (internal stop)      :: 38 of 620
            Pseudo Genes (multiple problems)  :: 54 of 620
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:39:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2554445
            Orig. end                         :: 2565342
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GG770539.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated scaffolds were added in May 2010.
FEATURES             Location/Qualifiers
     region          1..10897
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="11"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10897
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10897
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5897](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5897
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(473..1831)
                     /locus_tag="SSBG_RS10335"
                     /old_locus_tag="SSBG_01921"
     CDS             complement(473..1831)
                     /EC_number="3.5.3.13"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068159.1"
                     /locus_tag="SSBG_RS10335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01921"
                     /product="formimidoylglutamate deiminase"
                     /protein_id="WP_037869424.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5.8e-44, bitscore:
                     149.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLTYWLEHAWLGERTASGLVLTAGADGRIATLTEGVPGPPPGAV
                     PLRGLTLPGLANAHSHAFHRALRAATQRARGTFWTWRETMYGVAARLTPDTYHALARAV
                     YAEMALGGITAVGEFHYVHHQPDGTPYADPNAMGEALIEAAREAGVRLTLLDTAYLSAG
                     FGAPPEPHQLRFTDGDADAWARRVDRLRGDAHTVIGAAVHSVRAVPADQLATVAAWARE
                     RGAPLHVHLSEQPAENEACLAAHGMTPTQLLAAHGVLGPGTTGVHNTHLTDEDIRLIGG
                     SGTGTCMCPTTERDLADGIGPAVALQEAGSPLSLGSDSHAVLDLFEEARALELNERLRT
                     RTRGHWTAAALLEAASPGGHAALGRPDAGRLAPGAPADFTTLALDTVRTAGHGPELGAE
                     TAVFAGSAADVRHTVVGGEHVVRDGRHTRVADPAALLAETLAVPHTEGTDARA"
     misc_feature    complement(1854..1866)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            complement(1857..3056)
                     /locus_tag="SSBG_RS10340"
                     /old_locus_tag="SSBG_01922"
     CDS             complement(1857..3056)
                     /GO_function="GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amidines
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007823066.1"
                     /locus_tag="SSBG_RS10340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01922"
                     /product="allantoate amidohydrolase"
                     /protein_id="WP_008746106.1"
                     /transl_table=11
                     /translation="MWDTLAPLGRDKDSGGYRRFAWTPADRDVRDWFRQQAADRGLVYE
                     EDRNGNQWAWLGDPEGPDAVVTGSHLDSVPDGGAFDGPLGVVTAFAALDALRERGTAPR
                     KPLAITNFADEEGARFGLACVGSRLSAGALTKDAAFALHDADGVSLPRAMERAGFDPEA
                     VGADRARLNRVSAYVELHVEQGRALDLTGDPVGIASAIWPHGRWRFDWRGEANHAGTTR
                     LVDRHDPMLPYAETVLAARREAALAQAVATFGKLHVVPGGVNAIPSLVRGWLDSRAADQ
                     ETLDTVVTAIEEAARAAAAREGVELAVVRESFTPVVEFGHALREELARVLRTDGGEVPV
                     LPTGAGHDAGILSAVIPTAMLFVRNPTGVSHAPAEHAERADCHAGVTALADVLEELACR
                     "
     gap             3069..3168
                     /estimated_length=unknown
     gene            complement(3211..4890)
                     /gene="hutU"
                     /locus_tag="SSBG_RS10345"
                     /old_locus_tag="SSBG_01923"
     CDS             complement(3211..4890)
                     /EC_number="4.2.1.49"
                     /GO_function="GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hutU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007823068.1"
                     /locus_tag="SSBG_RS10345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01923"
                     /product="urocanate hydratase"
                     /protein_id="WP_008746107.1"
                     /transl_table=11
                     /translation="MSGPRPVRAPRGTELSTLGWQQEAALRMLQNNLDPEVAEHPDELV
                     VYGGSGKAARDWNSFDAMIRTLRTLKNDETMLVQSGRPVGVMRTHEWAPRVLIANSNLV
                     GDWANWEEFRRLEELGLTMYGQMTAGSWIYIGTQGILQGTYETFSAVAAKKFGGTLAGT
                     ITLTAGLGGMGGAQPLAVTMNDGVALCIDCDPRAIERRIEHRYLDVKADSVAHALELAT
                     AARDRREPLSIGLLGNAAEIVPQLLADGAPIDIVTDQTSAHDPLAYLPLGVDFADMAAY
                     AKRKPAEFTTRAREAMAKHVEAMVGFQDAGAEVFDYGNSIRGEAQLAGYERAFAFPGFV
                     PAYIRPLFCEGKGPFRWAALSGEASDIHKTDQALLDLFPENESLHRWIKMAQERVHFQG
                     LPARICWLGQGERDKAGARFNEMVASGELQAPIVIGRDHLDTGSVASPYRETEAMKDGS
                     DAIADWPLLNAMVNVASGASWVSIHHGGGVGMGRSLHAGQVTVADGTPLAAEKIRRVLT
                     NDPGMGVIRHVDAGYEEAEAVAEAKGVRIPMREGDAREGGAA"
     gene            5001..5897
                     /locus_tag="SSBG_RS10350"
                     /old_locus_tag="SSBG_01924"
     CDS             5001..5897
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068156.1"
                     /locus_tag="SSBG_RS10350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01924"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_008746108.1"
                     /sec_met_domain="AfsA (E-value: 1.3e-12, bitscore: 46.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRARPGDGRENDYYNDHTATPAKYDPLLLMEVLRQCGILTAHAYV
                     GAPADSSFVFDSATLRILCAEALTIRARPARATVDFQVRETKERAGRPVGATYVARLDI
                     EGSPAAEAVLECRWMPRQIWGKLRERSRSALDLTPRARFTGQRLPGYLVGRRSPQNVVL
                     TGATVGGTTGEGTAVVGHVVVDQGHLGLFDHPLDHISGAVMFEAFRQTALYAASEVHGL
                     APRGLFLEEIETRFVRVAEFELPTDCRARVGAGDESGVSVDLELVQEDRIAATARGRLG
                     RSLVPALAERETGLVHA"
     misc_feature    complement(5027..5041)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.45"
                     /tool="antismash"
     misc_feature    5028..5039
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.1"
                     /tool="antismash"
     misc_feature    complement(5044..5059)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.08"
                     /tool="antismash"
     gene            5894..6592
                     /locus_tag="SSBG_RS10355"
                     /old_locus_tag="SSBG_01925"
     CDS             5894..6592
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068155.1"
                     /locus_tag="SSBG_RS10355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01925"
                     /product="HAD family phosphatase"
                     /protein_id="WP_008746109.1"
                     /sec_met_domain="HAD_2 (E-value: 0.00037, bitscore: 19.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTGTTGEARGRASDMVVWFDFGGVLSPPLGELFRAFEKKTGIGP
                     EQLRLAMEDVGRDLGVPPLAPIESGTMPEAEWTALLAAALRRRWPGLDQSRARWDAFGR
                     QWFGGVEVNAPVAEAARRLGEAGFRVGVLSNNVREWEPYWRAVIEPAGELDFVVDSCLH
                     GVRKPEPAIYALAQRTAAAGASDCVLVDDLAENCRAAEEAGWTAVHFRDTAQALRELSA
                     VTGIRLCRLP"
     gene            6667..7869
                     /locus_tag="SSBG_RS10360"
                     /old_locus_tag="SSBG_01926"
     CDS             6667..7869
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068154.1"
                     /locus_tag="SSBG_RS10360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01926"
                     /product="cytochrome P450"
                     /protein_id="WP_008746110.1"
                     /sec_met_domain="p450 (E-value: 3.5e-12, bitscore: 44.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATSAPPQPSIDGIPNLDPVLTFVPLAARHRGGFPLIELPSGHTA
                     VHLTRYADVHRVLTDPSFGRTATNVEGGPSFLPTIMPKELLLNLDAPDHARMRGFVNAD
                     YSVAGVERLRPVVRELVAAKAERLRQEARPDLYATVLDTLPAEVNGRFLGIPDADIAYY
                     RPLGHTVQVASHEDVPGLVADFTELYTYLTELVTGARPRIEGGVIDRFLAARDSVEPPL
                     EDAELVGILLGSVLGADQNILSVASKIAYVLLCRPELWQRLAAEPALAPRLVDELIRLI
                     PLGNISAFPRIAGGRVELSEGVIEAGDVVYPDAFAANRDPGVFPEPLRVDPDRTGKRHL
                     QFGYGMHHCMGAALARMEIAEVLTTLAAEFPGLTLDADPATLSWDSGVILRRPVTLPVR
                     W"
     gene            complement(7913..8329)
                     /locus_tag="SSBG_RS10365"
                     /old_locus_tag="SSBG_01927"
     CDS             complement(7913..8329)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007823072.1"
                     /locus_tag="SSBG_RS10365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01927"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_008746111.1"
                     /transl_table=11
                     /translation="MPDAEGHPSVEEFDFQTVLKALSDPLRYHVVATLVRQPRDIERTC
                     TSFGLPVGKSTRTHHFRILREAGLVRQVDRGNSRSARLRREDIESRFPGLLRLVAENAP
                     LPEDAALGGQTPLAGDAVLAGDAALPGAEGLPAA"
     misc_feature    8357..8370
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.98"
                     /tool="antismash"
     gene            8476..9942
                     /locus_tag="SSBG_RS10370"
                     /old_locus_tag="SSBG_01928"
     CDS             8476..9942
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007823073.1"
                     /locus_tag="SSBG_RS10370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_01928"
                     /product="MFS transporter"
                     /protein_id="WP_008746112.1"
                     /transl_table=11
                     /translation="MASPTADPTRPAQAPPEPLKEAPAGPGKLTLIAVLLAVCVVPTSI
                     SGTAVALPDIGAGTGAAQGSASLQWVVNAFNLAFACFTLVWGSLADLIGRIRAFAIGAA
                     LFALASLACALAPNVYVLDGARALAGLGGAAIFACGSAILSSAFEGPARAKAFALFGTV
                     AGVGVALGPTVSGPAVDALGWRWIFGLQAIVLAVVLACVPVIARGVRESVGGGRLDVRG
                     AAVFVLAMAALTYAIVQGSAWGWTSAGTLGLLLGSLVLFAAFGALAKRTAAPLLDLSVI
                     RGRRFIAYTLVPVAASFGFVTQLTYLPSYLSTVAGYSASAAGTTMLLLTIPVLVLPMVG
                     AKLVERGTSPLAVVFASLLCLVVGDLALLVLGPDTSLAVMAPAMLVTGAGMGLSAGLVD
                     GQALAMVEPAKAGMAAGFINTLRLGSEAIAVAVFGSVLASLLSDGSGSGASAYDDAFHV
                     LLWVMAGVCLLLGLLVVSLARGGDRAEGAR"
     gene            9950..10444
                     /locus_tag="SSBG_RS35420"
     CDS             9950..10444
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="SSBG_RS35420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_267880259.1"
                     /transl_table=11
                     /translation="MTSAPSPSLSYEVAVVGGGPVGMLLAAELAHHGIAVAVIERNTRT
                     LDVPKAGTLHARTAQSLRRRGYLEGPEPTTALLESEQADAFHFAGLPGLTITGPAVEGE
                     PIVGRAQGDLERLFERVAVERGATVLRGHRVTALRGDGVNGADGSGAHGGRADGPGSAG
                     S"
ORIGIN
        1 tacgtcacct cgtcggtgtg acgcgcggcg agcgcgagcg cgcgggcctc gtcgtccacc
       61 gtgaggccgt acccggactt cgtctcctgc gtcgtcgtgc cctggcgcag gccctcggcg
      121 aggtggcggg ccaggttcgc ctccaggtcc gcgtcgctcg ccgcgcgcgt cgcggcgacg
      181 gtcgtacgga tgccgcccgc ctcgtagggg cggccggaca tgcgggcgtt gaactcgcgg
      241 gtgcggtcgc ccgcgaagac gaggtgggag tgcgagtcca cgaagccggg gacgaccgcg
      301 cgcccttcga ggtcgtggcg ggcgtccgcg gccggagcgg cgctcgcggg ccccgtccag
      361 gcgatccgct cgccgtccac gacgagcgcg gcgtcctcga tcacgccgag cgggccgccg
      421 tgcgcggggt cgttggtgac gagggagccg atgccggtga ggagggtgct ggtcatgcgc
      481 gcgcgtccgt tccttcggtg tgcgggaccg cgagggtctc ggcgagcagg gcggccgggt
      541 ccgcgacgcg ggtgtgccgt ccgtcccgta cgacatgctc gccgccgacg accgtgtgcc
      601 gcacgtccgc cgcgctgccc gcgaagacgg ccgtctcggc gccgagttcc gggccgtgtc
      661 ccgccgtgcg cacggtgtcg agcgcgaggg tcgtgaagtc ggcgggcgcg ccgggcgcga
      721 ggcggcccgc gtcggggcgg ccgagcgcgg cgtggccgcc ggggctcgcc gcttcgagga
      781 gcgcggcggc cgtccagtgg ccgcgtgtgc gggtgcgcag ccgctcgttc agctccagcg
      841 cgcgggcctc ctcgaagagg tcgagcacgg catggctgtc gctgccgagc gagagggggc
      901 tgcccgcctc ctggagggcg acggcgggcc cgatgccgtc cgcgaggtcg cgctcggtgg
      961 tcgggcacat gcacgtgccc gtgccgctgc cgccgatgag gcggatgtcc tcgtccgtga
     1021 ggtgggtgtt gtgcacgccg gtcgtgcccg gtcccaggac gccgtgcgcg gcgaggagct
     1081 gggtgggtgt catgccgtgc gcggccaggc atgcctcgtt ctcggcgggc tgttcggaga
     1141 ggtggacgtg caggggcgcg ccgcgctcgc gcgcccaggc ggcgacggtc gcgagctggt
     1201 cggccggtac ggcgcgtacg gagtggacgg ccgcgccgat gacggtgtgg gcgtcgccgc
     1261 gcagccggtc cacgcgccgg gcccaggcgt ccgcgtcgcc gtcggtgaag cggagctggt
     1321 ggggctcggg cggggcgccg aagccggcgg agaggtaggc ggtgtcgagc agcgtgaggc
     1381 gcacgcccgc ctcgcgcgcg gcctcgatga gcgcctcgcc catcgcgttg gggtccgcgt
     1441 agggggtgcc gtccggctgg tggtgcacgt agtggaactc gccgacggcg gtgatgccgc
     1501 cgagtgccat ctcggcgtac acggcgcggg cgagggcgtg gtacgtgtcc ggggtgaggc
     1561 gggcggcgac gccgtacatc gtctcgcgcc aggtccagaa cgtgccgcgc gcccgctggg
     1621 tggccgcgcg cagggcgcgg tggaaggcgt gcgagtgggc gttggcgagc ccggggaggg
     1681 tgaggccgcg caggggcacg gcgccgggcg gggggccggg gacgccttcg gtgagggtgg
     1741 cgatccggcc gtccgcgccg gccgtgagga cgagcccgga ggcggtgcgc tcaccgagcc
     1801 acgcgtgttc gagccagtac gtcagcggca cgccgcctcc tcccgtgcgg ggtgtgtcag
     1861 cggcacgcca gctcctccag tacgtccgcg agggcggtga cccccgcgtg gcagtcggcg
     1921 cgctccgcgt gctcggcggg ggcgtgcgag acgccggtcg ggttgcgcac gaacagcatc
     1981 gcggtgggga tgaccgcgga gaggatgccc gcgtcgtgtc ccgcgccggt ggggagcacg
     2041 gggacctctc ccccgtcggt gcgcaggacg cgggccagtt cctcgcgcag cgcgtggccg
     2101 aactcgacca cgggcgtgaa ggactcgcgt acgacggcga gttcgacgcc ctcgcgcgcc
     2161 gcggccgcgc gggcggcctc ctcgatcgcg gtgacgaccg tgtccagggt ctcctggtcg
     2221 gcggcgcgcg agtcgagcca gccgcggacg agcgagggga tcgcgttgac gccgccggga
     2281 acgacgtgga gcttgccgaa ggtcgcgacg gcctgggcga gcgcggcctc gcggcgcgcg
     2341 gcgaggaccg tctcggcgta cgggagcatc gggtcgtggc ggtccacgag ccgggtcgtg
     2401 cccgcgtggt tggcctcgcc gcgccagtcg aagcgccagc ggccgtgcgg ccagatggcg
     2461 ctggcgatgc cgaccggatc gccggtcagg tcgagcgcgc ggccctgttc gacgtggagt
     2521 tcgacgtagg cggagacgcg gttcaggcgg gcgcggtcgg cgccgaccgc ctcggggtcg
     2581 aagccggcgc gttccatggc gcgggggagg ctgacgccgt cggcgtcgtg gagggcgaag
     2641 gcggcgtcct tcgtgagcgc gcccgcgctg agccgcgagc cgacgcaggc gaggccgaag
     2701 cgggcgcctt cctcgtcggc gaagttggtg atcgcgaggg gcttgcgggg ggccgtgccg
     2761 cgctcgcgca gcgcgtcgag cgcggcgaag gcggtgacga cgccgagggg gccgtcgaag
     2821 gcgccgccgt cgggtacgga gtcgaggtgc gagccggtga cgacggcgtc ggggccctcg
     2881 gggtcgccga gccaggccca ctggttgccg ttgcggtcct cctcgtagac caggccccgg
     2941 tcggcggcct gctgccggaa ccagtcgcgg acgtcgcggt cggcgggtgt ccaggcgaag
     3001 cggcggtagc cgccgctgtc cttgtcgcgg ccgagcgggg cgagggtgtc ccacatgcgg
     3061 tcgaagtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gggggcgccc
     3181 cggccgtggc gccgcgcggg gtgggcgcgc tcacgcggcg ccgccctcgc gcgcgtcgcc
     3241 ctcgcgcatc gggatgcgga cgcccttcgc ctcggcgacg gcctcggcct cctcgtaccc
     3301 ggcgtccacg tgccggatga cgcccatgcc ggggtcgttg gtgaggacac ggcggatctt
     3361 ctcggcggcg agcggggtgc cgtcggcgac cgtgacctga ccggcgtgga gcgagcggcc
     3421 catgccgacg ccgccgccgt ggtggatcga gacccaggag gccccggagg cgacgttgac
     3481 catcgcgttg agcagcggcc agtccgcgat ggcgtccgag ccgtccttca tggcctcggt
     3541 ctcgcggtac ggggaggcga cggagccggt gtcgaggtgg tcgcggccga tgacgatggg
     3601 ggcctggagc tcgcccgagg cgaccatctc gttgaagcgc gccccggcct tgtcgcgttc
     3661 gccctggccg agccagcaga tacgggccgg gaggccctgg aagtggacgc gttcctgcgc
     3721 catcttgatc cagcggtgca gcgactcgtt ctcggggaag aggtcgagga gcgcctggtc
     3781 ggtcttgtgg atgtcgctcg cctcgccgga gagcgcggcc cagcggaagg ggcccttgcc
     3841 ctcgcagaag agcgggcgga tgtaggcggg gacgaagccg gggaaggcga aggcgcgctc
     3901 gtaccctgcg agctgggcct cgccccggat cgagttgccg tagtcgaaga cctcggcgcc
     3961 cgcgtcctgg aagccgacca tggcctcgac gtgcttcgcc atcgcctcgc gcgcgcgggt
     4021 cgtgaactcg gcgggcttcc tcttcgcgta cgcggccatg tcggcgaagt ccacgccgag
     4081 ggggaggtag gcgagcgggt cgtgggccga ggtctggtcg gtgacgatgt cgatgggggc
     4141 gccgtcggcg aggagctggg ggacgatctc ggcggcgttg ccgagcaggc cgatggagag
     4201 gggttcgcgg cggtcgcgcg cggcggtggc cagttccagg gcgtgggcga cgctgtcggc
     4261 cttcacgtcg aggtagcggt gctcgatgcg gcgctcgatg gcgcgcgggt cgcagtcgat
     4321 acagagcgcg acgccgtcgt tcatcgtcac ggcgagcggc tgggcgccgc ccatgccgcc
     4381 gagcccggcg gtgagggtga tggtcccggc gagggtaccg ccgaacttct tcgcggcgac
     4441 ggcggagaag gtctcgtagg tgccctggag gatgccctgg gtgccgatgt agatccacga
     4501 accggccgtc atctggccgt acatggtgag gcccagctcc tccaggcggc ggaactcctc
     4561 ccagttcgcc cagtcgccga cgaggttgga gttggcgatg aggacgcggg gggcccactc
     4621 gtgcgtgcgc atgacgccga cggggcggcc cgactgcacg agcatcgtct cgtcgttctt
     4681 gagggtgcgc agcgtgcgga tcatcgcgtc gaaggagttc cagtcgcggg ccgccttgcc
     4741 ggagccgccg tagacgacga gttcgtccgg gtgctcggcg acctccgggt ccaggttgtt
     4801 ctggagcatc cgcagcgcgg cctcctgctg ccagccgagg gtactcagct cggtgccgcg
     4861 aggggcgcgg acggggcggg gaccggacat gggctgcctc ctgtgactcg attgattcac
     4921 atactccact cctgaatagc cctagtcaat ggcgaccggg gaccgggcgc gggaagccgg
     4981 ggacggacct cgggcgccgg gtgcgcgcga ggcccggaga cggacgggaa aacgactact
     5041 acaacgacca cacggccacg cccgccaagt acgacccgct gctcctcatg gaggtgctgc
     5101 ggcagtgcgg catcctcacg gcgcacgcgt acgtgggcgc gccggccgat tcgagcttcg
     5161 tgttcgactc cgcgacgctg cggattctgt gcgccgaagc actgaccatc agggcccgcc
     5221 ccgcccgcgc cacggtggac ttccaggtgc gggagacgaa ggagcgcgcg ggcaggcccg
     5281 tcggcgccac gtacgtggcg cgcctcgaca tcgaggggag cccggccgcc gaggccgtgc
     5341 tcgaatgccg ctggatgccg cgccagatct gggggaagct ccgcgagcgc agccgctccg
     5401 ccctcgacct gaccccgcgc gcccgcttca cggggcagcg gctgcccggc tacctggtgg
     5461 gccggcgctc cccgcagaac gtcgtcctca cgggcgccac cgtcggcggg accaccggcg
     5521 agggcaccgc ggtcgtcggg cacgtggtcg tggaccaggg gcacctcggc ctcttcgacc
     5581 acccgctcga ccacatctcg ggggccgtga tgttcgaggc gttccggcag accgccctct
     5641 acgccgcgag cgaggtgcac gggctggccc cgcgcggcct cttcctggag gagatcgaga
     5701 cccggttcgt ccgggtggcg gagttcgaac tgcccacgga ctgccgggcg cgggtcgggg
     5761 cgggcgacga gagcggcgtg tccgtggacc tggaactggt ccaggaggac cggatcgcgg
     5821 cgacggcccg ggggcgcctg gggcgttcgc tggtccccgc cctcgcggag cgggagacgg
     5881 ggctggtgca cgcgtgagca ccggcacgac gggcgaggcc cgcgggcggg cctcggacat
     5941 ggtggtgtgg ttcgacttcg gcggtgtcct ctcgccgccg ctcggcgaac tcttccgcgc
     6001 cttcgagaag aagacgggca tcggcccgga gcaactacgc ctcgcgatgg aggacgtggg
     6061 gcgggacctc ggtgtgccgc cgctcgcgcc catcgagtcc ggcacgatgc cggaggcgga
     6121 gtggaccgcg ctgctcgctg cggcgctgcg gcggcgctgg ccgggactcg accagtcccg
     6181 cgcccggtgg gacgccttcg gccggcagtg gttcgggggc gtcgaggtca acgccccggt
     6241 cgcggaggcg gcacggcggc tgggcgaggc cggtttccgg gtcggcgtcc tgtcgaacaa
     6301 cgtccgcgag tgggagccgt actggcgggc cgtcatcgag cccgcgggcg agctggactt
     6361 cgtcgtggac tcctgcctgc acggcgtccg caagcccgaa ccggcgatct acgccctcgc
     6421 ccagcgcacg gcggcggcgg gcgcctcgga ctgcgtcctc gtggacgacc tggcggagaa
     6481 ctgccgggcc gccgaggagg ccggctggac ggccgtccac ttccgggaca ccgcgcaggc
     6541 gctgcgcgag ctgagcgcgg tcaccggcat ccgcctgtgc aggctgccct gacccggcac
     6601 gccgccccgc ctcccaccgc attcccagtg aaccgatttt ccacgtcacc cccacagaag
     6661 cgctccatgg ccacgtccgc gcccccgcag ccgagtatcg acggcatccc gaacctcgac
     6721 ccggtcttga ccttcgtccc gctcgccgcg cgccaccgcg gcgggttccc cctcatcgag
     6781 ctgccgagcg ggcacaccgc cgtccacctg acgcgctacg ccgacgtgca ccgggtcctg
     6841 acggacccgt cgttcgggcg caccgcgacc aatgtcgagg gcgggcccag cttcctgccg
     6901 acgatcatgc cgaaggaact gctgctcaac ctcgacgcgc ccgaccacgc ccggatgcgg
     6961 ggcttcgtca acgcggacta cagcgtcgcc ggggtcgagc ggctgcggcc cgtggtcagg
     7021 gaactggtcg cggccaaggc cgagcggctg cggcaggagg cgcgtcccga cctgtacgcg
     7081 acggtgctcg acacgctgcc cgccgaggtg aacgggcgct tcctcggcat cccggacgcc
     7141 gacatcgcct actaccgccc gctcgggcac acggtgcagg tcgcctcgca cgaggacgtg
     7201 ccggggctcg tcgccgattt caccgagctg tacacgtacc tcacggagct ggtcaccggc
     7261 gcgcggccga ggatcgaggg cggcgtgatc gaccgcttcc tcgccgcgcg cgactcggtg
     7321 gaaccgccgc tggaggacgc cgaactcgtc ggcatcctgc tcggctccgt cctcggggcg
     7381 gaccagaaca tcctgtcggt cgcgtccaag atcgcctacg tgctgctgtg ccgcccggag
     7441 ctgtggcagc ggctcgcggc ggagcccgcc ctcgcgccgc ggctcgtgga cgagctgata
     7501 cggctcatcc cgctcggcaa catctcggcc ttcccccgga tcgcgggcgg ccgggtggag
     7561 ctgagcgagg gcgtgatcga ggcgggggac gtggtctacc cggacgcctt cgcggccaac
     7621 cgcgaccccg gggtcttccc cgagccgctg cgcgtcgacc cggaccgtac cggcaagcgg
     7681 cacctccagt tcggctacgg gatgcaccac tgcatgggcg cggcgctcgc gcgcatggag
     7741 atcgccgagg tgctcacgac gctcgcggcg gagttccccg ggctgacgct cgacgccgac
     7801 ccggcgacgc tctcctggga cagcggcgtc atcctgcgcc gcccggtgac gctcccggtg
     7861 cgctggtagc acccgggccc tcgccctttc gggggacgcc gcgccggggc cgtcacgctg
     7921 ccgggagccc ctccgctccc ggcagcgcgg cgtccccggc gagcacggcg tccccggcga
     7981 gcggggtctg accgccgagc gcggcgtcct ccggcagcgg ggcgttctcg gcgaccagcc
     8041 gcagcagccc ggggaagcgg ctctcgatgt cctcgcggcg caaccgggcg ctgcggctgt
     8101 tgccccggtc cacctgccgg acgagtccgg cctcgcgcag gatgcggaag tggtgcgtac
     8161 gcgtcgactt gcccacgggc aggccgaagg acgtacaggt gcgctcgatg tcccgcggct
     8221 gccggacgag ggtcgcgacg acgtggtagc gcagcgggtc ggagagcgcc ttgagcaccg
     8281 tctggaagtc gaactcctcg acgctcgggt ggccttcggc atcaggcatg agtacgcctc
     8341 cgggggacga agttgaaaac catacgttcc gatccaaggt actactcgcg tgggaccttg
     8401 cgtacatgcc tggctgaggt tcgataaaga ccgaacctcg gtcgtaccgt ccccgcctca
     8461 cctccggagc tctccgtggc ctcacccacc gccgacccga cccgccccgc gcaggcgccc
     8521 cccgaaccgc tcaaggaagc cccggcaggg cccgggaagc tgaccctgat cgccgtgctg
     8581 ctcgcggtct gcgtcgtccc gacctcgatc tcggggacgg ccgtcgcgct ccccgacatc
     8641 ggcgccggga ccggggcagc gcagggctcg gcctcgctcc agtgggtcgt caacgccttc
     8701 aacctcgcct tcgcctgctt caccctcgtg tggggcagcc tcgccgacct catcggccgc
     8761 atccgggcct tcgccatcgg cgccgccctc ttcgcgctcg cctcgctggc ctgcgcgctc
     8821 gcccccaacg tgtacgtgct cgacggggcc cgcgcgctgg ccgggctcgg cggcgccgcg
     8881 atcttcgcct gcggcagcgc gatcctgtcc tcggccttcg aaggaccggc ccgggccaag
     8941 gcgttcgcgc tcttcggcac cgtcgcgggc gtcggcgtgg ccctcggccc gacggtctcg
     9001 ggaccggccg tggacgcgct cggctggcgc tggatcttcg ggctccaggc gatcgtcctg
     9061 gccgtcgtgc tcgcctgcgt cccggtcatc gcgcgcggcg tgcgggagtc cgtcggcggc
     9121 ggccgcctgg acgtgcgcgg cgcggccgtc ttcgtcctcg ccatggcggc gctgacctac
     9181 gccatcgtgc agggctcggc gtggggctgg acctcggccg ggacgctcgg cctgctgctc
     9241 ggctcgctcg tcctgttcgc cgccttcggg gcgctcgcca agcggaccgc tgcaccgctc
     9301 ctggacctct ccgtcatccg gggccgccgc ttcatcgcct acacgctggt gccggtcgcc
     9361 gcctccttcg gcttcgtcac ccagctcacc tacctgccct cgtacctctc gaccgtcgcc
     9421 ggctacagcg cctcggccgc cggtacgacg atgctcctgc tcacgatccc ggtgctggtc
     9481 ctgccgatgg tgggcgccaa gctcgtggag cgcggcacct cgccgctcgc cgtcgtcttc
     9541 gcctcgctgc tgtgcctcgt cgtcggggac ctcgcgctgc tggtcctcgg cccggacacc
     9601 tcgctcgcgg tcatggcgcc cgcgatgctc gtgaccggcg cgggcatggg cctgtccgcc
     9661 ggtctcgtcg acggtcaggc gctcgcgatg gtcgagccgg cgaaggccgg gatggccgcg
     9721 ggcttcatca acacgctgcg gctcggcagc gaggccatcg ccgtcgcggt gttcggctcc
     9781 gtgctcgcct cgctcctgtc ggacggctcc ggctcgggcg cctccgccta cgacgacgcc
     9841 ttccacgtcc tgctgtgggt catggccggg gtctgcctgc tgctgggcct gctcgtcgtc
     9901 tcgctcgcgc gcggcggcga ccgggccgaa ggggccaggt gagcactccg tgacctccgc
     9961 cccgtccccc tcgctctcct acgaggtcgc cgtcgtcggc ggcggccccg tggggatgct
    10021 cctcgccgcc gaactggccc accacggcat cgccgtcgcc gtgatcgagc ggaacacccg
    10081 gacgctcgac gtaccgaagg ccgggacgct ccacgcccgc accgcgcagt cgctgcggcg
    10141 gcgcggctac ctcgaaggcc ccgagccgac caccgcgctc ctggaatccg agcaggccga
    10201 cgccttccac ttcgcgggac tgcccgggct caccatcacg ggccccgccg tggagggcga
    10261 accgatcgtg ggccgggccc agggcgacct ggaacggctc ttcgagcgcg tcgcggtgga
    10321 acgcggggcg accgtcctgc gcggccaccg ggtcacggcg ctgcgggggg acggggtgaa
    10381 cggcgcggac ggctccgggg cgcacggggg ccgggcggac gggcccggtt cggcgggtag
    10441 ctagcgtcgt gcgcggacgg cgccccgccc cgtacgctgc gcgcctcctg ggtcatcggc
    10501 gcggacggcg cccgcagcct cgtgcgcgag gcgtccgagg tgacgagcga ggaacacccg
    10561 ccccgtacgg cggccgtgct cggcctcgtc gagctggacg accccttcgg cgcgccggcg
    10621 ggctggaccc cgaccccgcg cggctggacg gtcatcggcc ccaacccgta cgggcccagc
    10681 cgcgtcgccg ccttcgactt ctccgggccg cacgccgacc ggcgcacccc gctcacgctc
    10741 gaagagctgc gcgccacggt ctcgcgcatc gcgggccgcg acatccccat gcgcgacgcg
    10801 cgcttcctgg accggttcag cgactacgcc cggctcaccc acacctaccg ctcggggcgg
    10861 gtcctcctcg cgggcgacgc cgcccacgtc cacttcc
//
