LOCUS       NZ_GG770539            10468 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces sp. SPB074 supercont3.1, whole genome shotgun sequence.
ACCESSION   NZ_GG770539
VERSION     NZ_GG770539.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. SPB074
  ORGANISM  Streptomyces sp. SPB074
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     The genome sequence of Streptomyces sp. strain SPB74
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M.,
            Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D.,
            Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M.,
            Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I.,
            Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T.,
            Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D.,
            Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T.,
            White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R.,
            Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
REFERENCE   4  (bases 1 to 6808467)
  AUTHORS   Fischbach,M., Ward,D., Young,S., Jaffe,D., Gnerre,S., Berlin,A.,
            Heiman,D., Hepburn,T., Sykes,S., Alvarado,L., Kodira,C.D.,
            Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S.,
            Walsh,C.T., Lander,E., Galagan,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2008) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Arachne v. April 2007
            Genome Coverage                   :: 12x
            Sequencing Technology             :: ABI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 01:42:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,117
            CDSs (total)                      :: 6,045
            Genes (coding)                    :: 5,425
            CDSs (with protein)               :: 5,425
            Genes (RNA)                       :: 72
            rRNAs                             :: 6, 1 (5S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 620
            CDSs (without protein)            :: 620
            Pseudo Genes (ambiguous residues) :: 0 of 620
            Pseudo Genes (frameshifted)       :: 261 of 620
            Pseudo Genes (incomplete)         :: 378 of 620
            Pseudo Genes (internal stop)      :: 38 of 620
            Pseudo Genes (multiple problems)  :: 54 of 620
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:39:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1984109
            Orig. end                         :: 1994577
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GG770539.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated scaffolds were added in May 2010.
FEATURES             Location/Qualifiers
     region          1..10468
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10468
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10468
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5468](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5468)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            342..>515
                     /locus_tag="SSBG_RS07910"
                     /pseudo=""
     CDS             342..>515
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010271812.1"
                     /locus_tag="SSBG_RS07910"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPSAVFPGASQPEVPASLVEQLGSPALPSPLREIEEAAFARRGVR
                     LLLKRDDLVHPEI"
     gene            608..1627
                     /locus_tag="SSBG_RS30815"
                     /old_locus_tag="SSBG_02364"
     CDS             608..1627
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /locus_tag="SSBG_RS30815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_02364"
                     /product="recombinase family protein"
                     /protein_id="WP_008745669.1"
                     /transl_table=11
                     /translation="MDATTDPAKLCDLYLRRSNWQDDKTTLKKHERDLRDRAGREGLIV
                     RKVWVEEISAFKVGLVRNEFDNAIAAVTTDQVRHLLVWKLDRLSRQGMGQVGNVLNEFG
                     KRGARLIAHMDGLDSSIPQHRGLFAWLAEQARSESYNTSVRVRSTKAEKKATGAWPGGQ
                     PPYGLRARNRKTEHNPKEFSVARRIADALLQDRSAQAIAAELNSDGLRTRRGGMWRGST
                     ITQLAHSPAWAGLMPVHEQYVDKAGRERWRLTDEPFVGGDGKTVRVGKGVITVGERARI
                     LSGLRSRTTEILGNARRGKPGAQSLLSSFAKCGRCGGNMVKAGQAYRCYRRVQLRAVP"
     misc_feature    1184..1186
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1679..2449)
                     /locus_tag="SSBG_RS07915"
                     /old_locus_tag="SSBG_02363"
     CDS             complement(1679..2449)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745670.1"
                     /locus_tag="SSBG_RS07915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_02363"
                     /product="IS5-like element ISStsp8 family transposase"
                     /protein_id="WP_008745670.1"
                     /transl_table=11
                     /translation="MAGVITAAEPSWTAPFTGLSPRQFGKLVTVLRCEGADAVRKGRPW
                     SLSLEDRALLVAAYWRTNLTMRQLAPLFGVSKSAADRIIDHLGPMLALQPRKRFSRDTV
                     LIVDGTLVPTRDHTIAERSKNYRCSTNHQVVIDADTRLAVVVGRPLAGNRNDREAWEES
                     GAKAAVGKTLTIADGGCPGTGLVIPHRRERGQTELPDWKEEHNKSHKQVRARVEHVFAR
                     MKTWKILRDCRLKGDGVHHAMLGIARTHNLTLAG"
     gene            2517..3122
                     /locus_tag="SSBG_RS33115"
                     /old_locus_tag="SSBG_02362"
     CDS             2517..3122
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSBG_RS33115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SSBG_02362"
                     /product="hypothetical protein"
                     /protein_id="WP_008745671.1"
                     /transl_table=11
                     /translation="MTVLVRDLDRAISAAFMARVRSFKSEHETFKELANRWLAYDEPET
                     EARRIELVTGLTDARSRLEKLDNAYYVEGRFKGSAGEDRYSRMRDAIEQQLHSSEDALS
                     AIQAAVDLTMLKDAERLRSAWLSADLQTARMLLRVVLHSVTVLPPRGQGCKRNWIHLLT
                     DCCFHWVGESQQPLRRDSERIRGLVLFEEDQVSLNLAA"
     gene            complement(3342..3974)
                     /locus_tag="SSBG_RS33120"
     CDS             complement(3342..3974)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSBG_RS33120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158677753.1"
                     /transl_table=11
                     /translation="MNFLFVTYRFLWIALLASAVGVLGPVLSEVHNPAIHVAHVVLSAG
                     WFYAALAYFSGVGASGRLRAAVTGTGALVVAVIAYYVTKAAQGTFTNADLSDHTGNTTY
                     FSWDNFLSKILIWVCLSLLLGTLLGVAGASAWNARSGVIWRLVIPCIALAETVMRLQVR
                     SPSVETVNVYTWYVVLGISIVAAIGVVVHWALHLRRKDGGVFSRSEA"
     gene            complement(4172..4942)
                     /locus_tag="SSBG_RS07920"
                     /old_locus_tag="SSBG_02361"
     CDS             complement(4172..4942)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745670.1"
                     /locus_tag="SSBG_RS07920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_02361"
                     /product="IS5-like element ISStsp8 family transposase"
                     /protein_id="WP_008745672.1"
                     /transl_table=11
                     /translation="MAGVITAAEPSWTAPFTGLSPRQFGKLVTVLRCEGADAVRKGRPW
                     SLSLEDRALLVAAYWRTNLTMRQLAPLFGVSKSAADRIIDHLGPMLALQPRKRFSRDTV
                     LIVDGTLVPTRDHTIAERSKNYRCSTNHQVVIDADTRLAVVVGRPLAGNRNDREAWEES
                     GAKAAVGKTLTIADGGCPGTGLVIPHRRERGQTELPDWKEEHDKSHKQVRARVEHVFAR
                     MKTWKILRDCRLKGDGVHHAMLGIARTHNLTLAG"
     gene            complement(5001..5468)
                     /locus_tag="SSBG_RS30160"
                     /old_locus_tag="SSBG_02360"
     CDS             complement(5001..5468)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="SSBG_RS30160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_02360"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_050776738.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 7.8e-08,
                     bitscore: 31.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSRMLAVGAVSVMAVFGGFSMTGSAVATEQNSPQIFTATYTDSD
                     GVEPPAILLGPNGEKPTEWGVAVIPADVTGPIISPMTTKSVGGGTWSYEVGTDGINKAC
                     SSKYIHPSKKHSATAVIGSKADKVIKEKDIWANAYATAGLKFTCETYWGVY"
     misc_feature    5463..5475
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.65"
                     /tool="antismash"
     gene            5545..6353
                     /locus_tag="SSBG_RS32085"
                     /old_locus_tag="SSBG_05461"
     CDS             join(5545..5889,5892..6353)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558177.1"
                     /locus_tag="SSBG_RS32085"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="SSBG_05461"
                     /product="IS5 family transposase"
                     /protein_id="WP_234010412.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MVGLVERLVPDELWQLVQRVVPEAPSRPQGGGRRRHGDREVLAAI
                     VFVATSGCTWQQLPSASFGPSGATAHRRFSEWSKARVWAKLHRLVLDELGTRGELDWSR
                     CAIDSVNMRALKGDLTGPNPVDRGKYGSKIHLITERTGLPISVAISGANVHDSQALIPL
                     VKGIPPISSRRGPRRRRPGKLHADKGYDYRHLRQWLSGRGIRHRIARKGVETSQRLGRH
                     RWTIERTMSWLAGCRRLHRRYERKADHFLAFTSIACTLICYRRLTK"
     misc_feature    6483..6498
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.43"
                     /tool="antismash"
     misc_feature    complement(6518..6538)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.47"
                     /tool="antismash"
     gene            7477..8853
                     /locus_tag="SSBG_RS07945"
                     /old_locus_tag="SSBG_02356"
     CDS             7477..8853
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745676.1"
                     /locus_tag="SSBG_RS07945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_02356"
                     /product="IS1380-like element ISStsp3 family transposase"
                     /protein_id="WP_008745676.1"
                     /transl_table=11
                     /translation="MKHSIGSYPRVRVQDDGRQVVSQAGTVLFVETARKTGLDQVISAA
                     LAPWRKPRAVHDPGKVLLDVALAVALGGDCLADVAVLRAEPAAFGPVASDPTVSRLIDA
                     LAASGEKALQAIRSARSEVRDRVWSLAGENAPDTGGQVVVDLDGVLVIAHSDKEDAAAT
                     WKKTYGHHPLTAFVDHGPGGTGEPVAALLRPGNAGSNTAADHITTAQLALAQLPKRYRR
                     GRQTLIRTDSAGGTHDFVSWLARRGRWLSYPVGMTVTEAIHEHVLKAPASAWTAAVETN
                     GEVRDGAWVAELTGKLLDGWPKGMRLIVRKERPHPGAQLRITDADGMRITCFATNTTGR
                     PIAELELRHRLRARAEDRIRAARATGLRNLPLHTTAQNKVWLRIVQIALDLLAWMPMLA
                     LTGKARLWEPRRLRFRLFTAAGQLVTTGRRRILRLARHRPWTGHITAALERLALLPNPG
                     "
     gene            9511..9885
                     /locus_tag="SSBG_RS07950"
                     /old_locus_tag="SSBG_02354"
     CDS             9511..9885
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068644.1"
                     /locus_tag="SSBG_RS07950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSBG_02354"
                     /product="hypothetical protein"
                     /protein_id="WP_234010414.1"
                     /transl_table=11
                     /translation="MTKTDADNSDAPRLLPWNTSDGKPCFLVSEETRSWVARMADGIEE
                     VKLAMSAELLGHAMAVLQDRKATDHELRVIGELLTQSLGDIIRVGVSRGMRFGIPPASD
                     GGNGEGETDEHGEHVTGERS"
ORIGIN
        1 ccggcggcca cccagcgcgc gctggaggcc ctggaggccc acatcgtgac gcacggcgac
       61 ggtttcctgc cccgctgagc cgggcaggcg ccgccgagcc tggcggcgcc gtggtcggct
      121 ggacgggcgc ccaccgggcc ggggcgggcg ggccacgtcg agccggagtc gagccggaag
      181 ggggcccgcc gggccgaagg gtgtcccctg aaccggggca cccgccacgc cggggcgggt
      241 gtcgccgtcc cggagggccc ccgcagagcc gcgcccggtc ccatcgaggc cgcgtgcggc
      301 ccgtccgtac gcgcgagcga ttcgggtacg acaatgggcg gatgccgtcc gctgtattcc
      361 ccggtgcctc gcagccggag gtgcccgcct cgctcgtcga gcagctgggc tcccccgctc
      421 tgccctctcc cctgcgggag atcgaggagg cggccttcgc ccgtcgcgga gtgcgtctcc
      481 tcttgaagcg ggacgatctg gtgcacccgg agatttctga gaacaggccg actggtgtag
      541 gtggccgctg accagggatt aattggctgg acggtcgccc acaccttcac tgaaggtggt
      601 tctcaccatg gatgcgacca cagaccccgc caagctgtgc gatctgtacc tgcggcggag
      661 caactggcag gacgacaaga cgaccctcaa gaagcacgag cgggatcttc gagaccgggc
      721 aggtcgagaa gggctaatcg ttcgcaaagt ttgggtcgag gagatatccg ctttcaaggt
      781 cgggcttgtg cggaacgagt tcgataacgc gattgcggcc gtgacgaccg accaagtgcg
      841 acacctgttg gtatggaagc tcgaccggct cagccgtcaa gggatgggcc aggtcggcaa
      901 tgtactaaac gagttcggga agcggggcgc tcgcctcatc gctcatatgg acggcttgga
      961 cagcagcatt ccacagcacc gtggattgtt tgcttggctg gcggagcagg cgcgttccga
     1021 gtcttacaat acttctgttc gcgtgcgtag tacgaaggca gaaaagaagg ccaccggagc
     1081 ttggccgggc ggccaaccgc cgtatgggct gcgcgcacgc aacagaaaga ccgaacacaa
     1141 cccgaaggaa ttctccgttg cgcgccgcat cgctgacgcc ttgttacagg atcggtcggc
     1201 acaggccatc gccgctgaac tgaattcaga cgggctgcga acgcgacgcg gcgggatgtg
     1261 gcgagggtcc acgatcacac agttggcgca ctcccccgcg tgggcgggcc tcatgccggt
     1321 gcacgaacaa tacgtagaca aggcagggcg ggagcgctgg cgccttaccg atgagccgtt
     1381 cgtgggaggg gatggaaaaa cggtccgtgt cggcaagggg gtcataacgg ttggcgagcg
     1441 agcgcggatt ctctccggtc tacgctctcg tacgacagaa atcctgggaa atgcacgccg
     1501 agggaagccc ggggctcaat cgctcctgtc cagtttcgcc aagtgcggcc ggtgcggggg
     1561 gaacatggtc aaggcgggac aggcttaccg ttgctaccgg cgagttcaac taagggctgt
     1621 cccgtaaatg atctccgagt cgcctcgggc gtggttgatc gctgtgcggc ccgctgccct
     1681 atccggcgag ggtgaggttg tgcgtccggg cgattccgag catggcgtgg tggactccgt
     1741 ctcctttgag gcggcagtcg cggaggatct tccaggtctt catgcgggcg aagacatgct
     1801 cgacgcgggc gcggacctgc ttgtgggact tgttgtgttc ctctttccag tccggaagtt
     1861 cggtctggcc gcgctcgcgc cggtgaggga tgacgagtcc ggtaccgggg cagccgccgt
     1921 cggcgatcgt gagggtcttg ccgacggcgg ccttggcgcc ggattcctcc catgcttcgc
     1981 ggtcgttgcg gttcccggcg agcggccggc cgaccacgac ggccaggcgg gtgtcggcgt
     2041 cgatgacgac ctggtggttg gtggagcagc gatagttctt cgaccgttcg gcgatcgtgt
     2101 gatcccgggt cgggaccagg gtgccgtcca cgatcagcac ggtgtccctg gagaaccgct
     2161 tgcggggctg gagagcgagc atcggtccga ggtggtcgat gatccggtcc gccgccgact
     2221 tggacacccc aaacaacggg gcgagctgtc gcatggtcag attcgtgcgc cagtaggccg
     2281 cgaccagcag ggcccggtcc tccagcgaaa ggctccacgg ccggcccttg cggacggcgt
     2341 ccgcgccctc acacctgaga accgtcacca gcttcccgaa ctgccgcggg ctcagcccgg
     2401 tgaacggggc tgtccaggac ggctccgccg ccgtgatcac accagccacg ccatgatcat
     2461 ctcatccgtg accagcagtt acgggacagc ccttaggtaa atcggcttgc cagggaatga
     2521 ccgtactcgt tcgggacctg gaccgcgcca tatctgcggc cttcatggca cgcgtacgct
     2581 catttaaaag cgaacacgag acctttaagg aattggccaa ccgctggttg gcttacgatg
     2641 aacccgaaac cgaggcacga cgaatcgaac tcgtcaccgg cctgacggat gctcggtcgc
     2701 gtctggagaa actggacaac gcctattacg tggaaggccg ctttaaggga agcgccgggg
     2761 aagatcgcta ctccaggatg cgagacgcca tcgagcagca gttgcactcc tccgaagacg
     2821 cgttgtcagc aattcaagca gcggtggacc tcaccatgct taaggatgcg gaacgtctac
     2881 gctccgcctg gctttccgct gatctgcaaa ctgcccggat gctgctgcgg gtcgtgctcc
     2941 actccgttac cgtccttccc ccaagggggc agggttgcaa aaggaactgg atacacctac
     3001 tgacagactg ctgctttcac tgggttggcg agagtcagca acctttgcgc cgagattcgg
     3061 agcgtattag ggggctagtc ctattcgaag aggaccaagt cagtctgaac ttggcggctt
     3121 agacgttagc cccctgatcc gcacttcggg cacgcggccg gccgcagaga ctttgggctg
     3181 gagctgccat ggggcgagtg aacagggggc cttacgctcg ccagcgaatc gggcaggctt
     3241 tggagctgcc cgcaatagcc cattccgccc cctgctctta tgaagatcgt gagagcaggg
     3301 ggttgcaaat gaatacacca acctacttac ggttcccgca ttcacgcttc cgagcgcgag
     3361 aagacgcctc catcttttcg acgaagatgc agcgcccaat gaacaacaac cccaatggcc
     3421 gccactatcg agatacccaa cactacatac cacgtataca cgttaacagt ttccaccgac
     3481 ggagatctga cttgcaaacg catgaccgtt tcagccagtg caatacacgg aataaccaaa
     3541 cgccaaataa cccccgatcg cgcattccag gcggaagcac ctgcaacccc caatagcgta
     3601 ccaagcaaca aagaaaggca aacccaaatg agaattttcg aaaggaagtt gtcccacgaa
     3661 aaataggttg tgttgcccgt gtgatcactt aggtctgcgt tagtgaaggt tccctgggcc
     3721 gccttagtga cgtaataagc aataacagcc acaacaagag cgccagtgcc agtcacggcc
     3781 gcgcgcaatc ggccactcgc cccaacgcct gaaaaatacg ccagcgcggc atagaaccaa
     3841 ccggcactca atacgacatg tgccacatgt atagccgggt tatgcacttc actgagaaca
     3901 gggccaagca cacctactgc ggaagccaaa agagcaatcc acaagaaacg gtatgtgaca
     3961 aacaaaaagt tcacccttac gataaaaccg acattacgca cagtgcatac ccggcgtcgt
     4021 tacgcttcac gaacacgagc acaggagaag cgttggcgtc acccaccgaa tactatgggt
     4081 cacgccaacg cctaaaacaa gactaagggc tgtcccgtaa atgatctccg agtcgcctcg
     4141 ggcgtggttg atcgctgtgc ggcccgctgc cctatccggc gagggtgagg ttgtgcgtcc
     4201 gggcgattcc gagcatggcg tggtggactc cgtctccttt gaggcggcag tcgcggagga
     4261 tcttccaggt cttcatgcgg gcgaagacat gctcgacgcg ggcgcggacc tgcttgtggg
     4321 acttgtcgtg ttcctctttc cagtccggaa gttcggtctg gccgcgctcg cgccggtgag
     4381 ggatgacgag tccggtaccg gggcagccgc cgtcggcgat cgtgagggtc ttgccgacgg
     4441 cggccttggc gccggattcc tcccatgctt cgcggtcgtt gcggttcccg gcgagcggcc
     4501 ggccgaccac gacggccagg cgggtgtcgg cgtcgatgac gacctggtgg ttggtggagc
     4561 agcgatagtt cttcgaccgt tcggcgatcg tgtgatcccg ggtcgggacc agggtgccgt
     4621 ccacgatcag cacggtgtcc ctggagaacc gcttgcgggg ctggagagcg agcatcggtc
     4681 cgaggtggtc gatgatccgg tccgccgccg acttggacac cccaaacaac ggggcgagct
     4741 gtcgcatggt cagattcgtg cgccagtagg ccgcgaccag cagggcccgg tcctccagcg
     4801 aaaggctcca cggccggccc ttgcggacgg cgtccgcgcc ctcacacctg agaaccgtca
     4861 ccagcttccc gaactgccgc gggctcagcc cggtgaacgg ggctgtccag gacggctccg
     4921 ccgccgtgat cacaccagcc acgccatgat catctcatcc gtgaccagca gttacgggac
     4981 agcccttagc ggaggagggg tcagtagacg ccccagtacg tctcgcaggt gaacttgagt
     5041 ccggctgtcg cgtatgcgtt cgcccagatg tccttctcct tgatcacctt atcggcctta
     5101 ctcccgataa cggcggtcgc cgagtgcttc ttgctggggt ggatgtactt ggatgaacac
     5161 gccttgttaa tgccgtccgt gccgacttcg tagctccagg ttccgccgcc gacgctcttc
     5221 gtcgtcatgg gcgagatgat cgggccagtc acatctgccg gaattacagc gaccccccac
     5281 tcagtgggct tttcgccgtt gggcccaagc aagatggctg gcggctcgac cccatccgag
     5341 tcggtgtagg tagccgtgaa gatctgcggg ctgttctgct cggtcgcaac tgcggaacca
     5401 gtcatgctga atccgccgaa gaccgccatc acagagaccg cgccgaccgc caacattctg
     5461 ctgcgcattt tccaactcct gcgagttcgc cgtgaatttt ccttagaagc catctcattt
     5521 gggtgactcg gtagtctatg agtcatggtg gggcttgttg agcggctggt gccggacgaa
     5581 ttgtggcagt tggtccagcg ggtggtgcct gaggcgccgt cgcggccgca gggtggtggt
     5641 cggcgtcggc acggtgaccg ggaagtgctg gccgcgatcg tcttcgtggc cacgtcgggt
     5701 tgtacctggc agcagctgcc ttcggcgtcg ttcggcccgt ccggagcgac tgcccaccgg
     5761 cggttctcgg agtggtcgaa ggccagggtg tgggccaagc tccaccgcct ggtcctcgac
     5821 gagctcggta cgcgcgggga gctggactgg tcgcggtgcg cgatcgactc ggtgaacatg
     5881 cgggccttga aaagggggac ctgacaggtc cgaatcctgt cgaccggggc aagtacgggt
     5941 cgaagatcca cctgatcacc gagcggaccg gtctgcccat atccgtcgca atctccggtg
     6001 ccaacgtcca cgacagccaa gcccttatcc cgctcgtgaa gggcataccg cctatcagct
     6061 cccgccgggg cccccgtcga cgccgacccg gaaagctcca cgccgacaag ggatacgact
     6121 accgccacct gcggcaatgg ctgtccggac gcggaatccg gcatcgcatc gcccgcaaag
     6181 gcgtcgagac ctcgcagcgg ctcggccgac accgctggac catcgaacgc accatgtcct
     6241 ggctcgccgg ctgccgacga ctccaccgac gctacgaacg caaagccgac cacttcctcg
     6301 ccttcaccag catcgcctgc accctcatct gctaccgcag actcaccaaa tgagatgact
     6361 tcttagagac atcccgcatc ctgaaccaag cagtcttcaa gcgcattgaa aggttgtcta
     6421 aaaaacacgc ctttcactca acagtcgccg ccaccagaat gaaatctggc cccataagac
     6481 cattcgcacc tttttcaaca ttggcgctag cgaagagaaa tatgccttgg ctatatgtgc
     6541 acggatatac gggcatcaat tgcattattt gccccatctc gggctccaag ggtgacaata
     6601 aaaacaagac aatcaccagg gattcaatcg cgtacggaag gagcaggtgc taaccgatct
     6661 tcacggtcta ccgagccctg catgggcctg ccgttccctg tatccgcgac cacccggact
     6721 tgtgcggcac ggcgcaccaa gacggaaatg gcgcacttgt gcgaatttgc cgcagagggt
     6781 gaagggcaag actcgtaggc cgtccgcccc agccacgact gctcttcgtc acgtacgtca
     6841 ggtactgggc tggcggagaa gacaggagac gcgctcttgc gcctgggtgc cgcttgccgg
     6901 gcggctgccg accggacggg accggccgga ggccgcatgc ccggccgagg cggcaagacc
     6961 gcccggcgcg ccgaaggcgc gcccttgagc aagtagagaa aagttacgcc gattctccgg
     7021 gccgcgttct cccccgcccg ccagatcctg cacgcacgtt gacgagggat gtcgcttggc
     7081 acgggagcag ccctacggcc ctgttgagag cttccggctg aggtcaccca gcgggcggcc
     7141 ggttgaagcg gctgagggcc cgccgggacg tgtcggcggt ctcgtaggtg agggaccaag
     7201 gaccgctgtt gacggtaaag gacttgtcga acccgaccca caggccatcc atactccggc
     7261 cggtcatctc aacgaggagt tgcaaggccc cgtggtagac ggctcctctg tagtaagcct
     7321 ctgcggctgt gcgctcggtc caggtgcccg ttagcctcgc gctttcatga agcgggctgt
     7381 ccggcaggcg gtcaggaaag cagaaagtgc ctctgaccag caagaatgag gattgtcgag
     7441 gtccttgttc ctgccaccgc cggaggcact tcccagatga agcactctat cgggtcctat
     7501 ccccgtgtcc gcgtccagga cgacggtcgc caggtcgtct cccaggccgg gacggtcctg
     7561 ttcgtcgaaa cggcccgcaa aaccggtctt gaccaggtca tatcggcagc tctggcgccg
     7621 tggcgcaagc cgcgggccgt ccacgatcct ggcaaggtcc tcctggatgt cgccctggcg
     7681 gtcgcactgg gcggggactg cctcgcggat gtcgccgtgc tgcgggccga gccggccgcc
     7741 ttcggtccgg tcgcctccga cccgaccgtc tcccgcctga tcgacgccct cgccgcctcc
     7801 ggcgagaaag ctttgcaggc catccggtcc gcacgctccg aagtccgtga ccgtgtctgg
     7861 tcgttggccg gtgagaacgc cccggacacc ggcgggcagg tcgtcgtcga tcttgacggc
     7921 gtcctcgtga tcgcgcactc cgacaaggag gacgcggccg cgacctggaa gaagacctac
     7981 ggtcaccatc cgctgacggc cttcgtcgac cacggcccgg gcggaaccgg cgagccggtc
     8041 gccgccctcc tcaggccggg caacgcgggc tcgaacacag cggccgacca catcaccacc
     8101 gcccagctcg ccctggccca gctgcccaag cgttaccggc gagggcggca gacgctgatc
     8161 cgcacggact ccgccggcgg cacccacgac ttcgtgtcct ggctcgcccg gcgaggacga
     8221 tggctgtcct acccggtcgg aatgacggtc accgaggcga tccacgagca tgtgctgaag
     8281 gcccccgcat cggcctggac ggcggccgtc gagaccaatg gcgaggtccg tgacggggcc
     8341 tgggtcgccg agctcaccgg caagctgctg gacggctggc ccaagggcat gcgactgatc
     8401 gtccgaaagg aacggcccca ccccggcgcc cagttgagga tcacggacgc ggacggcatg
     8461 cggatcacgt gcttcgcgac caacaccacc ggtcggccga tcgccgaact cgagctccgt
     8521 caccggctac gggcccgggc ggaggacagg atccgggccg cccgggccac cggcctgcgc
     8581 aacctgcccc tccacaccac agcccagaac aaggtctggc tccggatcgt ccagatcgcc
     8641 ctcgacctgc tggcctggat gcccatgctc gcgctcaccg gcaaggccag gctctgggag
     8701 ccccgccgcc tgcggttccg cctgttcacc gcggccgggc agctcgtgac caccggccgt
     8761 cggcggatcc tccgcctggc ccggcaccgg ccctggaccg gccacatcac cgccgcactc
     8821 gaacggctcg cactcctgcc gaatcccggc tgaccagcgg attcacccat ccctacgaca
     8881 gctcttcacc acccggagca gtggaacccg gcgccacccc gagacgcact cgggccctcg
     8941 gcctgcccag cctcagctca cggcacgaaa acaggccacc gactgcgtcg gcggcccgtc
     9001 acgaaacttc gaggttagtg ccgggccatc cttggtgaag tccaggaaga tcggggagtc
     9061 gttggacccg ggcaggcttt cgccgtgcag tcggttgccc aactgccgta ccactacgta
     9121 gtggtgccgg gtcacctgcc cccgcttcgt gctctcgtag gtgtaacggc tgtgccagat
     9181 tcccgagagg ctgtcagcat cgggctggcc tccactcaac gcctggaccg acagccccag
     9241 ggcttgggct atgggagcga gccaatcccg tggggtgcgc cgtccggttt cgtacctgta
     9301 aatctcctgc ctccctaact tttctgggcc cgcgcctgcg actcggcacg cttctcttgc
     9361 caatcgctcc tgcgaccaac cgagttgtag acgccggtcg cgaatgcgtt ccccgatatt
     9421 catacctgcc attgtggcct acatctggcc tacaagttgg cccacacccg gccgctggcc
     9481 gccccgtcac aaggcgcaca ggatcgagcc atgacgaaga cagacgctga caactcagac
     9541 gcccccaggc tgttgccgtg gaacacgtcg gacggaaagc cctgcttcct ggtatccgag
     9601 gaaacgcgca gttgggtggc ccggatggcc gacggcatcg aagaagtgaa gctcgccatg
     9661 agcgccgagt tgctgggcca tgccatggcg gtactccagg accgtaaggc gacggatcac
     9721 gaactacgcg tcatcgggga gcttttgacg cagtcccttg gcgacatcat ccgcgttggc
     9781 gtaagtcgcg ggatgcggtt cggaatcccg cccgcatcgg atggcggcaa cggcgagggc
     9841 gagaccgacg aacatgggga gcacgtcacg ggtgagcggt catgacggcg ctccgccgca
     9901 tgatctggag catggctgga cctttgctga tcatcgcggt atccgcgtcc tgcggcttcg
     9961 tgctgggcgt agcactcgcc cacccgaact gaaccactcc acgcgctcca gggccccatc
    10021 ggtgaccgcc ggtcggggcc ctgtcgcttg cttcgacctg cccccttgca acctgaacca
    10081 gtgggcgtac agtcatccgg gaacaaatgg cgcaagctcg tccccgtgct cgccgggctg
    10141 acgccggggc gcaccctcgt gaccttcggc ggcgcctggt ccagtcatct gcgggccacg
    10201 gcgaccgccg ggcggctcct cggggtgccc acggtggggc tggtacgggg cgacgaactc
    10261 gccgcgcgcc cgctcaaccc cgcgctggag cgctgccgca cggacgggat gcgcctccac
    10321 ttcgtggacc gctcccgcta ccggcgcaag cgggagcccg agatcgcgcg ctccctgctg
    10381 cgcgaggccg gggtcaccgc cccgtacctc gtgccggagg gcggcagcgg tccggcggcc
    10441 gtacggggct gcgcggaact gggccggg
//
