LOCUS       NC_013928              10231 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Streptococcus mutans NN2025, complete sequence.
ACCESSION   NC_013928
VERSION     NC_013928.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus mutans NN2025
  ORGANISM  Streptococcus mutans NN2025
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Maruyama,F., Kobata,M., Kurokawa,K., Nishida,K., Sakurai,A.,
            Nakano,K., Nomura,R., Kawabata,S., Ooshima,T., Nakai,K., Hattori,M.,
            Hamada,S. and Nakagawa,I.
  TITLE     Comparative genomic analyses of Streptococcus mutans provide
            insights into chromosomal shuffling and species-specific content
  JOURNAL   BMC Genomics 10, 358 (2009)
   PUBMED   19656368
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013587)
  AUTHORS   Nakagawa,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2008) Contact:Ichiro Nakagawa Tokyo Medical and
            Dental University, Graduate School of Medical and Dental Science,
            Section of Bacterial Pathogenesis; 1-5-45 Yushima, Bunkyo-ku, Tokyo
            113-8510, Japan
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 00:52:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,974
            CDSs (total)                      :: 1,890
            Genes (coding)                    :: 1,830
            CDSs (with protein)               :: 1,830
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 21 of 60
            Pseudo Genes (internal stop)      :: 25 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:57:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1620096
            Orig. end                         :: 1630327
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010655.1.
            The complete genome sequence of S. mutans NN2025 was performed by
            Dr. M. Hattori and Dr. A. Yamashita, Department of Computational
            Biology, Graduate School of Frontier Sciences, The University of
            Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8561, Japan This work
            was done in collaboration with Ichiro Nakagawa, Fumito Maruyama
            (Tokyo Univiersity), Mitsuhiro Kobata (Osaka University), Ken
            Kurokawa (Nara Institute of Science and Technology), Keishin
            Nishida , Atsuo Sakurai (Tokyo University), Kazuhiko Nakano, Ryota
            Nomura, Shigetada Kawabata, Takashi Ooshima (Osaka University),
            Kenta Nakai (Tokyo University), Shigeyuki Hamada (Nihon
            University), and supported by KAKENHI (Grant-in-Aid for Scientific
            Research) on Priority Areas Applied Genomics from the Ministry of
            Education, Culture, Sports, Science and Technology of Japan. This
            clone was isolated from a Japanese patient with dental caries in
            Osaka University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10231
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10231
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10231
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5231](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5231)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            61..552
                     /locus_tag="SMUNN2025_RS07850"
                     /old_locus_tag="SmuNN2025_1532"
     CDS             61..552
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997911.1"
                     /locus_tag="SMUNN2025_RS07850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1532"
                     /product="YgjV family protein"
                     /protein_id="WP_012997911.1"
                     /transl_table=11
                     /translation="MLFSALGAFCLAYSSFTKEKQHMLLWQISDYIFTIIANLLLGGYT
                     GALTISVSIVRNALILKKKNTKVITICLVLIQIALGTYVNNLGLVGYLPIISSVSYTLT
                     TFLTSKMQFLRWLIIENMSLWFIYDLTINAYPAVCMDILIILSTLLALYKHRKKAGHSL
                     "
     gene            complement(658..1479)
                     /locus_tag="SMUNN2025_RS07855"
                     /old_locus_tag="SmuNN2025_1533"
     CDS             complement(658..1479)
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179: hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351286.1"
                     /locus_tag="SMUNN2025_RS07855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1533"
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="WP_002277959.1"
                     /transl_table=11
                     /translation="MVKLIAIDMDGTLLNSQKEIPQENIAVIQEAARSGYKIVLCTGRM
                     QTGVEPYFKQLGLAAEQEYAILNNGCSLHTINRDWQLLTYHDLNFNGVSYLYDLLEGYP
                     EIDLTLTADRDYLVLADQVSELVAYDASLVFTQAQTVDLNDVKHAAKPVFQAMYLGESD
                     RLDAFQQKFECKLAEKFTTVRSQPYIFEVMPQSITKATGLKELAQKLRISRTDIMAIGD
                     ALNDLEMLKAAGFSVAMGNASPEVKAAADFVTDSNDDAGVAQAIAKYVLNK"
     gene            complement(1726..1929)
                     /gene="copZ"
                     /locus_tag="SMUNN2025_RS07860"
                     /old_locus_tag="SmuNN2025_1534"
     CDS             complement(1726..1929)
                     /codon_start=1
                     /gene="copZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263706.1"
                     /locus_tag="SMUNN2025_RS07860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1534"
                     /product="copper chaperone CopZ"
                     /protein_id="WP_002264873.1"
                     /transl_table=11
                     /translation="MEKTYHIDGLKCQGCADNVTKRFSELKKVNDVKVDLDKKEVTITG
                     NPSKWSLKRALKGTNYELGAEI"
     gene            complement(1942..4170)
                     /locus_tag="SMUNN2025_RS07865"
                     /old_locus_tag="SmuNN2025_1535"
     CDS             complement(1942..4170)
                     /EC_number="7.2.2.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264872.1"
                     /locus_tag="SMUNN2025_RS07865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1535"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_002277960.1"
                     /transl_table=11
                     /translation="MSEEVFLIDGMTCASCAINVENAVKKLDGIESAVVNLTTEKMTID
                     YDAAKVSEADVTKAVAGAGYGAKVYDPTTAESQEDREEHKLAGIKKRLLWTSIFTIPLF
                     YIAMGSMVGLPLPNFLAPSSAPLTYAMVLLLLTIPVIVLSWSFYDNGFRSLFKGHPNMD
                     SLVSLATTAAFLYSLYGTYHVYLGHTHHAHHLYYESVAVILTLITLGKYFETLSKGRTS
                     DAIKKLMHLSAKEATLIRDGEEIKVPIEQVQIRDQILVKPGEKIPVDGRVLSGHSAIDE
                     SMLTGESIPIEKMADSPVYAGSINGQGSLTFEAEKVGNETLLSQIIKLVENAQQTKAPI
                     AKIADKVSAVFVPVIITIAILTGLFWYFVMGQDFTFSMTISVAVLVIACPCALGLATPT
                     AIMVGTGRAAENGILYKRGDVLELAHQINTIVFDKTGTITQGKPEVVHQFSYHDRTDLV
                     QVTASLEALSEHPLSQAIVDYAKKEGTRLLAVEDFTSLTGLGLKGCVADETLLVGNEKL
                     MRQENISLEQAQADFKAATAQGQTPIFVASDGQLLGLITIADKVKNDSAATVKALQNMG
                     VEVAMLTGDNEETAQAIAKEVGITFVISQVLPQEKTQAILDLQAEGKKVAMVGDGINDA
                     PALATADIGISMGSGTDIAMESADIVLMKPAMLDIIKALKISRATIINIKENLFWAFIY
                     NVLSVPIAMGVLYLFGGPLLDPMIAGLAMSFSSVSVVLNALRLKVVKL"
     gene            complement(4167..4610)
                     /locus_tag="SMUNN2025_RS07870"
                     /old_locus_tag="SmuNN2025_1536"
     CDS             complement(4167..4610)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264871.1"
                     /locus_tag="SMUNN2025_RS07870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1536"
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="WP_002277961.1"
                     /transl_table=11
                     /translation="MTSISNAEWEVMRVVWAKQMTSSSEIIAILSRTYCWSASTIKTLI
                     TRLSEKGYLTSQRQGRKYIYSSLISEEEALEQQVSEVFSRICVTKHQALIRHLVEETPM
                     TLSDIEKLEALLLSKKANAVPEVKCNCIVGQCSCYEHLEVTSK"
     misc_feature    4656..4671
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.41"
                     /tool="antismash"
     misc_feature    4682..4698
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.54"
                     /tool="antismash"
     misc_feature    4687..4700
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.34"
                     /tool="antismash"
     misc_feature    complement(4802..4816)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.87"
                     /tool="antismash"
     gene            complement(5001..5231)
                     /locus_tag="SMUNN2025_RS07875"
                     /old_locus_tag="SmuNN2025_1537"
     CDS             complement(5001..5231)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320866.1"
                     /locus_tag="SMUNN2025_RS07875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1537"
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_002275977.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 7.4e-16,
                     bitscore: 55.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTQAFEQFNVMDNEALSTVEGGGMIRCALGTAGSAGLGFVGGMG
                     AGTVTLPVVGTVSGAALGGWSGAAVGAATFC"
     gene            5499..5726
                     /locus_tag="SMUNN2025_RS10490"
     CDS             5499..5726
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287918.1"
                     /locus_tag="SMUNN2025_RS10490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002287918.1"
                     /transl_table=11
                     /translation="MEIEVDKLISSAQQPIVPIFKEYRQAYFENNRFLVDGKSLRWTYN
                     PNEGLTPIFVLLQQLTSRQKKLFCLLNSFF"
     gene            complement(5706..6059)
                     /gene="rbfA"
                     /locus_tag="SMUNN2025_RS07885"
                     /old_locus_tag="SmuNN2025_1538"
     CDS             complement(5706..6059)
                     /GO_function="GO:0019843 - rRNA binding [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rbfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274895.1"
                     /locus_tag="SMUNN2025_RS07885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1538"
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="WP_002274895.1"
                     /transl_table=11
                     /translation="MSNHRIERVGMEIKREVNEILQKKVRDPRVQGVTITDVQMSGDLS
                     LAKVYYTIMSDLASDNQKVQIGLEKAKGTIKRELGKNLTMYKIPDLTFIKDESIEYGNK
                     IDQMLRDLEKRIK"
     misc_feature    complement(6268..6285)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            complement(6293..9043)
                     /gene="infB"
                     /locus_tag="SMUNN2025_RS07890"
                     /old_locus_tag="SmuNN2025_1539"
     CDS             complement(6293..9043)
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003743 - translation initiation factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="infB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285798.1"
                     /locus_tag="SMUNN2025_RS07890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1539"
                     /product="translation initiation factor IF-2"
                     /protein_id="WP_002268773.1"
                     /transl_table=11
                     /translation="MSKKRLYEIAKEIGKESKEIVEKAKSLGLEVKSHASSVEESDAKR
                     IVESFTVSVEPKAVTPTSKVEKEAKAQEGSVAAEPKAAATKPAGRPRPQNRNFKAEREA
                     RAKAEAERRQNNGERRNQNKGQNNRQKDNRSHGSQDRRNDNRNNRNRQNDNRRDNRNHF
                     QNRQEASKSQPTGPRFDFKARAAALKAEQNAEYSRQSETRFHEAQEAKRQAAQAKEKAK
                     KLNQKEQPTVEAAATAAPQAQPQTVEQVTHPAAVDTRRKKQARPDKSRDFSHENEDGPK
                     QNKHKKNRNKQNQVRNQKNSNWNKKNKKSKNNRNHNANLKPVTERKFHELPKEFEYTEG
                     MTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDADTIELLMVDYGIEAHQKVEVDTA
                     DIERFFVEDDYLNPKNMVERAPVVTIMGHVDHGKTTLLDTLRNSRIATGEAGGITQHIG
                     AYQIEEGGKKITFLDTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKA
                     ADVPIIVAINKIDKPGANPERVIGELAEYGVISTAWGGDSEFVEISAKFGQNIEELLET
                     VLLVAEIQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLHVQDPIVVGNTFGRV
                     RAMTNDLGRRVKVAAPSTPVSITGLNEAPMAGDHFAVYEDEKAARAAGEERAKRALLKQ
                     RQLTHRVSLDNLFDTLKAGEVKSVNVIIKADVQGSVEALAASLLKIDVEGVKVNVVHSA
                     VGAINESDITLAEASNAVIIGFNVRPTPQARQQAEADEVEIRLHSIIYKVIEEVEDAMK
                     GMLDPEFEEKIIGEALIRETFKVSKVGTIGGFMVTNGKITRDSSARVIRDGVVVFDGKL
                     ASLKHYKDDVKEVGNGQEGGLMIENYNDIKIDDTIEAYIMEEIKR"
     gene            complement(9064..9366)
                     /locus_tag="SMUNN2025_RS07895"
                     /old_locus_tag="SmuNN2025_1540"
     CDS             complement(9064..9366)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262601.1"
                     /locus_tag="SMUNN2025_RS07895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1540"
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="WP_002262601.1"
                     /transl_table=11
                     /translation="MNNSKKLSNLLGLAQRAGRVISGEELVVKAIQTGQAQLIFLAKDA
                     GSNLTKKVTDKSNYYNIEVSTVFSALELSIAIGRNRKVLAIVDTGFSKKMRTFME"
     gene            complement(9359..9655)
                     /locus_tag="SMUNN2025_RS07900"
                     /old_locus_tag="SmuNN2025_1541"
     CDS             complement(9359..9655)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /locus_tag="SMUNN2025_RS07900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_1541"
                     /product="YlxR family protein"
                     /protein_id="WP_002263922.1"
                     /transl_table=11
                     /translation="MAKTRKIPLRKSLVSGEIIDKRDLLRIVKTKEGDVFIDPTGKKNG
                     RGAYIKLANEEALEAKKRKVFNRSFSMEIPESFYDELIAFVDHKVKRRELGLE"
     misc_feature    complement(10030..10044)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.66"
                     /tool="antismash"
ORIGIN
        1 tgaagagtat taatttttga caatcaaaaa tggtaacttt aatgaattct tccaattgca
       61 atgctttttt cagccctcgg tgctttttgc ctcgcttatt cttcttttac taaagagaaa
      121 cagcacatgc tactttggca aatcagcgac tatattttta cgattattgc taatctttta
      181 ctgggtggct atacaggcgc cctaaccatt agtgtttcca tcgtcagaaa tgcacttatt
      241 cttaagaaaa agaatacaaa agttattacc atctgtttag tcctcattca aattgcttta
      301 ggtacttatg tgaataactt gggcttagtc ggttacttgc ccattatttc ttctgtctct
      361 tacactttga caaccttttt aacatccaaa atgcaatttt tgcgctggtt gattattgaa
      421 aacatgtctc tctggttcat ttatgacctc actatcaacg cctaccctgc tgtttgcatg
      481 gatattttga tcattctctc aacactgctg gctttatata aacatcgaaa aaaagcagga
      541 cattccttgt gaattggctt tgtcctgctt cctattctta tttaattgat cctgaacttt
      601 ctattttcta gtgttaagca gatgaaaacg atttcccagt tcctttttca tgatttatca
      661 cttatttaag acatacttgg caatagcttg agcaacacca gcatcatcat tagaatcagt
      721 cacaaaatct gctgcagcct taacttctgg actagcattt cccatagcca cactgaaacc
      781 tgctgctttg agcatttcaa gatcattaag tgcatctcct attgccataa tatcagtcct
      841 tgaaattctt aatttctgag ctaattcttt caaaccagta gcttttgtaa tgctttgagg
      901 catcacttca aaaatgtaag gctgacttct tacagtagta aatttttctg ctaatttgca
      961 ctcaaatttt tgctggaaag catctaagcg atcgctctct cctaaataca tagcctgaaa
     1021 aactggttta gcagcatgct ttacatcatt taagtccaca gtctgtgctt gcgtaaatac
     1081 cagactcgca tcataagcta ctaattctga tacttgatca gccaagacca gataatccct
     1141 atcagcagtc aatgttaaat cgatttcagg ataaccctca agcaaatcat acaaataaga
     1201 tactccatta aagtttaaat catgataagt cagtaactgc caatctctat taatggtgtg
     1261 aagagagcag ccattattta gaatcgcata ttcttgttca gcagctaagc ctaattgttt
     1321 aaagtagggc tcaacaccag tctgcatacg gcctgtgcaa agaacaattt tatagcccga
     1381 acgggctgct tcttgaataa cagcaatatt ttcttgcggg atttcttttt gactattcaa
     1441 aagcgtgcca tccatatcaa tagcaatcag tttaaccata atcatataac cttttctaaa
     1501 acattccact atctagtgta tcacaaatcg aggaaactgg cacagcaagt tattataatt
     1561 ctgtatattc tacaataatt gaaacagata actatgacaa tgctataaat tcattttaca
     1621 gcagaaaaaa aactggtttc ccagtctttt ccctctttgt gaccatctga taagatgcaa
     1681 tgaaataact gtgtctttac agtttttaag gtatatgaaa cctacttaaa tttctgctcc
     1741 caattcataa ttggttcctt tcagtgctcg tttaagagac cacttgcttg gatttcctgt
     1801 aatcgtgact tcttttttat caaggtcaac tttgacatca ttgactttct ttaattctga
     1861 aaagcgtttg gtgacattgt cagcgcagcc ttggcatttt aagccatcaa tatgatatgt
     1921 tttttccatg ataattctcc tttataattt tacaacttta agacgcaggg catttagaac
     1981 aacagaaaca gagctaaagc tcatggctag accagcaatc attggatcca gcaaaggtcc
     2041 tccaaagaga taaagtactc ccatagcaat aggaactgac agaacattat aaataaatgc
     2101 ccagaaaaga ttttctttaa tattaataat agtagcacga ctaattttca gtgccttaat
     2161 aatatcaagc attgcaggtt tcatcaagac aatatccgcg gactccatgg caatatctgt
     2221 tccagagccc atggaaatgc caatatctgc tgtcgcaagt gccggagcgt cattaatacc
     2281 gtccccaacc atggcaactt tcttgccttc cgcctgcaaa tcaagaatgg cctgtgtttt
     2341 ttcctgcggc aaaacttgac tgataacaaa agtaatcccc acttctttgg caatagcctg
     2401 cgcagtttct tcattatcac ccgtcagcat ggccacttca acccccatat tctgtaaagc
     2461 tttaactgtt gctgcactgt cattctttac cttatccgca atcgtaatca gtcctaacaa
     2521 ttgaccatca ctggcaacaa aaatgggtgt ttgtccctga gctgttgctg ccttaaaatc
     2581 tgcttgagcc tgttctagag agatattttc ttgacgcatc aatttttcat taccaacaag
     2641 caaagtttca tcagcaacac agcctttcag tcctagccct gttagagaag taaagtcttc
     2701 cactgcaagt aaacgagtcc cttctttttt agcataatca acaatggcct gactaagggg
     2761 atgttcagat aatgcttcta aggaagctgt cacttgcact aaatcagttc gatcatgata
     2821 agaaaattga tgaacaactt ctggtttacc ttgagtaata gtgcctgttt tatcgaaaac
     2881 aattgtatta atctgatgtg ccaattccaa gacatcacca cgtttataaa ggattccatt
     2941 ttcagcagca cgacctgtac caaccataat agccgttggc gttgcaagac ccaaagcaca
     3001 agggcaggca atgacaagaa cagcgacact gattgtcatt gaaaaggtaa agtcttgtcc
     3061 catgacaaaa taccaaaaga gaccagtcaa aatagcaatc gttataataa caggtacaaa
     3121 gacagcagat accttatcag caatcttagc aatgggtgcc ttagtttgct gagcgttctc
     3181 aaccaattta ataatttgtg aaagcaaggt ttcattgcca actttttcag cctcaaaagt
     3241 cagacttccc tgaccattga ttgacccggc ataaacaggg ctatccgcca ttttttcaat
     3301 aggaatagat tctcctgtta acatggattc atcaatagca gaatgccctg acaggactcg
     3361 accatccaca ggtatttttt caccgggttt gactaaaatt tgatctctga tttgcacttg
     3421 ctcaatagga accttaatct cctcgccatc acgtatcaga gtagcctctt tagcagacaa
     3481 atgcattaat ttttttatcg catctgaggt tcgacctttc gatagggttt caaagtattt
     3541 ccctaaagta atgagagtta aaataacagc taccgattca taatagagat ggtgagcatg
     3601 atgtgtatgt cccaagtaaa catggtaagt tccataaaga ctgtaaagaa acgccgctgt
     3661 tgttgctaag gacactaatg agtccatatt aggatgacct ttaaaaagcg atctaaaacc
     3721 attgtcatag aagctccaac ttaacacgat aaccggaatt gtcaaaagaa gcaaaaccat
     3781 cgcataagtg agcggagcgc tgcttggtgc taaaaagtta ggtaagggca agccaaccat
     3841 acttcccata gcaatataaa aaaggggaat ggtaaaaata gaagtccaca aaagacgttt
     3901 tttaatacct gctaacttat gttcttcacg atcctcctga ctttcagccg tcgttgggtc
     3961 ataaacttta gcgccatagc ctgcaccagc aactgcctta gtaacatctg cttcactaac
     4021 cttagcggca tcgtaatcta tcgtcatttt ttcagttgtc aaattgacta ctgcgctttc
     4081 aataccgtct agttttttaa cggcattttc aacattgatg gcacaagagg cacaggtcat
     4141 gccatctatc aaaaatactt cttcactcat tttgatgtca cctccaaatg ttcatagcaa
     4201 gaacattgcc cgacaataca attacactta acttcaggca cagcatttgc ttttttggac
     4261 aatagcagag cttccaattt ttcaatatca gacaaagtca taggcgtttc ctcaactaag
     4321 tgtctaatca aagcttgatg cttcgtgaca caaatgcgcg agaaaacttc cgacacttgc
     4381 tgctctaatg cctcctcttc tgaaatcaaa ctagaataga tgtattttct tccttgacgc
     4441 tgactggtca aatagccttt ttctgataaa cgcgtgatca acgttttaat tgttgaagcg
     4501 gaccagcaat aagtccgact taagatagca ataatttcac tgctgctagt catctgctta
     4561 gcccagacaa cacgcattac ttcccattcc gcatttgaaa tagatgtcat ttgagctcct
     4621 ttcatctaca tttgtagata tattataacc tctttgtcta catttgtcaa caaaaactga
     4681 tttttaattt tttgacaaaa tatgacccta cttatcagct ttttaaaaga gcaaaaaaag
     4741 aggcgagaaa ccttagtttc tgcctcttca ctcaaacaac gaacagttct tcaataataa
     4801 tttttcaaaa tgatacagtc ctcattagct cttgtgattc ttgttaattt taaaccataa
     4861 gactaacaaa cctaacagac ctagtaaatt aatgctataa atgattggcg agagctcctt
     4921 aaaaagattt tttactgcca taaataacaa gaataataaa ggcagacttc cgatcaataa
     4981 ggatcttcta tttttcatgt tcaacaaaaa gtagcagcac ctacagctgc tccagaccag
     5041 cctcctaaag ccgctccaga tactgtacca acgactggga gagtaactgt accagctccc
     5101 atacccccta caaatcctaa acctgcagaa ccagctgtgc caagtgcaca tctaatcata
     5161 ccaccaccct caacagttga aagtgcttca ttatccatta cgttaaattg ttcaaatgct
     5221 tgtgtattca tatgatagat acctcttttc catttttaat tttttgtctg gttagccaac
     5281 cgacatctct aatatagcaa attatttttc aaaatggagc aaaatatcct aaacggtagc
     5341 tattttgtcc taaacggtta aaaatgacgt tataatgcct gtaataatag tcaataaaaa
     5401 ttaaaaatag ttttcgccta ctcgcatcgc aactgactac aaacatactg gagcacgaca
     5461 agctcaaaaa agtcaaaagg cttgggaggc cttctgagat ggaaatagaa gtagataagc
     5521 tgatatcgtc agcacaacaa cctattgttc cgatttttaa agagtataga caggcctact
     5581 tcgaaaataa tcgttttttg gttgatggaa aaagccttcg ttggacttat aatcctaacg
     5641 aaggcttgac acctattttc gtacttttac agcaattaac aagcagacag aagaaacttt
     5701 tttgtttact taattctttt ttctaaatcc cgcagcattt ggtcaatctt attaccatac
     5761 tcaatagatt catctttgat aaaagttaaa tcgggaattt tatacatggt caaatttttt
     5821 ccaagttcac gtttaatggt ccctttagcc ttttcaagac ctatttgaac tttctgatta
     5881 tcagaagcaa ggtcactcat gatagtatag taaacctttg ccagagataa atcacctgac
     5941 atttggacat cagtaatagt gacaccttga acacgtgggt cacgtacctt cttttgcaaa
     6001 atctcattaa cttcgcgctt aatttccata ccaacacgtt caatacgatg attagacatt
     6061 ctcttttcct ttcttttaat tttacaattt tcttttttca ccacgacaaa aaatatcttg
     6121 taaaagctca cttcattcgc tcatcttctg aaaaaagcta ggccagatga cctagcttta
     6181 ttgtttatct ttgaatttca aaagaaatca tatgacgttc atctaaggca acacgacaac
     6241 ggctgtactt gcgtatgcct agaagtgtta acaaaggagt aactaaatat tattatcttt
     6301 taatttcttc cataatgtaa gcctcaatag tatcatcaat tttgatgtca ttgtaatttt
     6361 caatcatgag accgccttct tgaccattac caacctcttt aacatcatct ttataatgtt
     6421 tcaaactagc aagcttaccg tcaaagacaa ccacaccatc acgaataaca cgagcacttg
     6481 aatcacgcgt aattttacca tttgttacca tgaagccgcc aatagttccc actttggaaa
     6541 ctttgaaggt ctcacgaata agcgcttcac caataatttt ttcttcaaac tctggatcaa
     6601 gcataccttt catggcatct tctacttctt caattacctt ataaataatg gaatgcaaac
     6661 gaatttcaac ttcatctgct tcggcctgct gacgggcctg tggagttggg cgaacattga
     6721 aaccaatgat aacagcatta gaagcttctg ccaaggtgat atctgattca ttgatggcac
     6781 caacagctga gtggaccaca ttaactttca caccttcaac gtcaatcttc aaaagagagg
     6841 ctgcaagagc ttcaacggaa ccttgcacat ctgctttgat aataacatta acagatttaa
     6901 cctcaccggc tttgagcgta tcaaagagat tgtcaagact gacacgatga gtcaattgac
     6961 gttgtttcag cagggcacgt ttagctcttt cttccccagc agcacgcgca gccttttcat
     7021 cctcataaac ggcaaaatgg tcacctgcca ttggggcttc attcaaacca gtgatagaga
     7081 ctggcgttga tggagcagca accttaacac gacgtccaag gtcattagtc atagcacgca
     7141 cacgtccaaa tgtgttccca acaacgattg gatcttgaac atgcagcgtt ccttgttgta
     7201 ctaaaagcgt cgctactgca ccttttcctt tatcaaggcg agcttcgata actgtaccaa
     7261 tagcacgaac agttgggtca gctttcagtt cttggatttc tgctactaga agaacagttt
     7321 ctaaaagttc ttcaatattt tgaccgaatt tagctgaaat ttcaacaaat tcagaatcgc
     7381 cgccccaagc agttgaaata acgccatact cagctaactc accgatgaca cgttctggat
     7441 tggcgcccgg cttatcaatt ttattaatag caacaataat tggaacatca gcagccttgg
     7501 agtggttaat agcttcaatc gtttgtggca taacaccatc atcagcagct acaataagaa
     7561 tggtaatatc agttaccgaa gcgccgcgtg cccgcatact tgtgaaggct gcatgtccgg
     7621 gtgtatcaag gaaagtaatt ttctttccac cttcttcaat ttgataggcc ccaatatgtt
     7681 gggtaattcc tccagcttca cctgtcgcta tacgggaatt acgcaatgta tcaagcaatg
     7741 ttgtcttacc atggtcgaca tgccccataa tcgttacaac gggcgcacgt tcaaccatat
     7801 ttttcggatt aagataatca tcttcaacga agaaacgttc aatatcagct gtatcaactt
     7861 ccactttttg atgtgcttca atgccataat caaccattag gagttcaatc gtatcagcat
     7921 ccaaagattg gttttgagtt gccataactc ccatcataaa gagttttttt acaatttcgg
     7981 caggctcacg cttaattcgt tttgcaattt cagcaaccgt cataccttct gtatactcaa
     8041 attcctttgg tagttcatgg aatttgcgtt cagtcacagg tttcagattg gcattatgat
     8101 tacggttatt tttagatttt ttatttttct tattccaatt actatttttt tgatttctca
     8161 cttggttttg tttattccga tttttcttat gtttattttg ttttggtccg tcttcattct
     8221 catgtgaaaa atcacgtgat ttatctgggc gagcctgttt tttgcgacgt gtatccacag
     8281 ctgctggatg tgtcacttgt tcaactgtct gaggctgagc ttgtggcgct gcagttgctg
     8341 cagcttcaac cgtcggctgc tctttctgat ttagcttctt agctttttct ttagcttgag
     8401 cggcttgacg tttagcttct tgagcttcat gaaaacgtgt ttcactctga cgcgaatatt
     8461 ccgcattttg ttctgctttt aaggctgcag cacgtgcttt gaaatcaaaa cgcggtcctg
     8521 tcggctggga ttttgatgct tcttgtcgat tttgaaaatg attacgatta tcgcgacgat
     8581 tgtcattttg acgattgcga ttattgcggt tatcattacg acgatcttgg gaaccatgac
     8641 ttcgattgtc tttttgccga ttgttctgtc ctttattttg gtttcgacgt tcaccattat
     8701 tttgacgacg ttcagcctct gctttggcac gcgcttctcg ttctgcttta aaattacgat
     8761 tttgcggtct tggacgccct gcaggctttg tagcagctgc tttaggttcc gctgcaacag
     8821 atccttcttg tgctttggct tctttctcaa ctttagaagt tggagtaaca gccttgggtt
     8881 ccacagagac agtaaaactt tctactatac gtttagcatc actttcttcc acgcttgaag
     8941 catggctttt aacttctagg cccaatgatt ttgccttttc aacaatttct ttactttctt
     9001 tgccaatttc cttggcaatt tcgtataatc ttttcttcga caaatcttgt cctcctattc
     9061 tttttattcc ataaaggtcc tcattttctt tgaaaatcca gtatccacaa tagcaagaac
     9121 ttttctgttt ctgccaatag cgatacttaa ttccagtgct gaaaacactg tggagacttc
     9181 tatattataa tagttacttt tatccgttac ttttttagtt agattcgaac cagcatcttt
     9241 ggctaaaaag atgagctgtg cctgtccagt ttgaatagct ttaacaacta attcttcacc
     9301 tgaaataaca cgtcctgctc tctgggccaa acctaataag tttgataact tttttgaatt
     9361 attcaagacc taactctctt cttttaacct tatgatcaac aaaagcaatt aattcatcat
     9421 aaaagctttc aggaatttcc atggaaaaac tgcgattaaa gacttttctt tttttagctt
     9481 ctaaagcttc ttcgttagca agtttaatat aagcccctcg cccatttttc ttaccagtag
     9541 gatcaataaa aacatcacct tccttggttt taacaatccg aagcaaatca cgtttatcaa
     9601 taatctctcc tgaaacaaga gactttctta aaggtatttt tcttgtcttt gccatcaaac
     9661 acctctgact ttctattttg catcttctgc tgcaaattct tgagagagtt cagaaacttc
     9721 ctcagcagga gcctcttcat aagtagcctc ttgggtttca tcttgtgctg cccactcggc
     9781 ttccatttct tcaaattcac tagcagactt aatatcaata cgatagccag tcaaatgagc
     9841 ggctaaacgg acattttgac cacgacggcc aatagcaagt gacagcttat tgtctggtac
     9901 aacaactgtt gcatgtttgt catcttcttc atcaaagaga acttgatcaa cctcagcagg
     9961 agcaacagca ttataaatga attctgctgg atcatcaacc cactcaataa cgtcaacatt
    10021 ctcttccatt ggaacccaaa gtccagtttt ttgatcaaaa cgtttaggat ggaatttact
    10081 ggtcactttc ttaatgttgg cacctttgcg gccaactata gttccaatag catcaacatt
    10141 aggattatga ctgcgaactg caaccttcgt tcgatcacca gcttcacgtg ccacgctcat
    10201 aatttcaaca gtgccatcaa aaacttcagg a
//
