LOCUS       NC_013928              10547 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Streptococcus mutans NN2025, complete sequence.
ACCESSION   NC_013928
VERSION     NC_013928.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus mutans NN2025
  ORGANISM  Streptococcus mutans NN2025
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Maruyama,F., Kobata,M., Kurokawa,K., Nishida,K., Sakurai,A.,
            Nakano,K., Nomura,R., Kawabata,S., Ooshima,T., Nakai,K., Hattori,M.,
            Hamada,S. and Nakagawa,I.
  TITLE     Comparative genomic analyses of Streptococcus mutans provide
            insights into chromosomal shuffling and species-specific content
  JOURNAL   BMC Genomics 10, 358 (2009)
   PUBMED   19656368
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013587)
  AUTHORS   Nakagawa,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2008) Contact:Ichiro Nakagawa Tokyo Medical and
            Dental University, Graduate School of Medical and Dental Science,
            Section of Bacterial Pathogenesis; 1-5-45 Yushima, Bunkyo-ku, Tokyo
            113-8510, Japan
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 00:52:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,974
            CDSs (total)                      :: 1,890
            Genes (coding)                    :: 1,830
            CDSs (with protein)               :: 1,830
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 21 of 60
            Pseudo Genes (internal stop)      :: 25 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:57:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 101160
            Orig. end                         :: 111707
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP010655.1.
            The complete genome sequence of S. mutans NN2025 was performed by
            Dr. M. Hattori and Dr. A. Yamashita, Department of Computational
            Biology, Graduate School of Frontier Sciences, The University of
            Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8561, Japan This work
            was done in collaboration with Ichiro Nakagawa, Fumito Maruyama
            (Tokyo Univiersity), Mitsuhiro Kobata (Osaka University), Ken
            Kurokawa (Nara Institute of Science and Technology), Keishin
            Nishida , Atsuo Sakurai (Tokyo University), Kazuhiko Nakano, Ryota
            Nomura, Shigetada Kawabata, Takashi Ooshima (Osaka University),
            Kenta Nakai (Tokyo University), Shigeyuki Hamada (Nihon
            University), and supported by KAKENHI (Grant-in-Aid for Scientific
            Research) on Priority Areas Applied Genomics from the Ministry of
            Education, Culture, Sports, Science and Technology of Japan. This
            clone was isolated from a Japanese patient with dental caries in
            Osaka University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10547
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10547
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10547
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5547](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5547)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            287..508
                     /locus_tag="SMUNN2025_RS00510"
     CDS             287..508
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268624.1"
                     /locus_tag="SMUNN2025_RS00510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002268624.1"
                     /transl_table=11
                     /translation="MANLELSKLSQIEQLLNCLLALAKQNQNGQNLTIVTQKELLNILQ
                     ISPNTLKSWERTGLNRLEPPIERTRTVY"
     gene            596..1857
                     /locus_tag="SMUNN2025_RS00515"
                     /pseudo=""
     CDS             596..1857
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /locus_tag="SMUNN2025_RS00515"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="tyrosine-type recombinase/integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MQKEIIFHNNKKVTKTTKNNGTISYTQRGVYLGIDIKTGKKVTSL
                     IIAKTLRSLDRKITQAKLDFEARGSTLKETVAINTFEELAEHGFLVLSRGCHHKIRLIV
                     LEITLTPTLFLNLVTINQKK"
     gene            2330..2914
                     /gene="rpoE"
                     /locus_tag="SMUNN2025_RS00520"
                     /old_locus_tag="SmuNN2025_0088"
     CDS             2330..2914
                     /EC_number="2.7.7.6"
                     /GO_function="GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rpoE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292635.1"
                     /locus_tag="SMUNN2025_RS00520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0088"
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="WP_002272892.1"
                     /transl_table=11
                     /translation="MELEVFAGQEKSELSMIEVARAILEERGRDKEMYFSDLVNAIQVY
                     LEKSDADIREALPFFYSDLNTDGSFIPLGENKWGLRSWYGIDEIDEEIVTLEEDEDGAP
                     KHKRKRVNAFMDGDEDAIDYSDDDPEDESFNTAEENTEYDEEDPDDEKSEVESYDSEIN
                     EIIPDEDLDENVDLDEEDDDYSDDEEDEEGE"
     gene            3048..4658
                     /locus_tag="SMUNN2025_RS00525"
                     /old_locus_tag="SmuNN2025_0089"
     CDS             3048..4658
                     /EC_number="6.3.4.2"
                     /GO_function="GO:0003883 - CTP synthase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315012.1"
                     /locus_tag="SMUNN2025_RS00525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0089"
                     /product="CTP synthase"
                     /protein_id="WP_002277781.1"
                     /transl_table=11
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYIN
                     IDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERK
                     GEYLGATVQVIPHITDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQMKA
                     DVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAGQGIK
                     NKLAQFCDVAPEAVIESLDVVHLYQIPLNLQAQNMDQIVCDHLKLDVPVADMTEWSAMV
                     DKVMNLKKKTKIALVGKYVELPDAYLSVVEALKHSGYVNDTAIDLNWINANEVTPETVT
                     DLLGDADGIIVPGGFGHRGTEGKIEAIRYARENDVPMLGICLGMQLTCVEYARHVLNLE
                     GANSAELDPDTKYPVIDIMRDQIDIEDMGGTLRLGLYPCKLKSGSKTASAYSNQEVVQR
                     RHRHRYEFNNAFRQQFEEAGFVFSGVSPDNRLVEVVELSDKKFFVAAQYHPELQSRPNR
                     PEELYTAFITAAVENSQAH"
     gene            complement(5001..5258)
                     /locus_tag="SMUNN2025_RS00530"
                     /old_locus_tag="SmuNN2025_0090"
     CDS             complement(5001..5258)
                     /GO_function="GO:0005186 - pheromone activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268322.1"
                     /locus_tag="SMUNN2025_RS00530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0090"
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="WP_002268322.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.8e-07,
                     bitscore: 28.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTLVLEQFETLDDDYLAQVQGEGWLGAVAGGVTGALAGAKAGGA
                     AALPVMCIPGFGWATEAGVVATSTIAGAVLGAGVGYNTPL"
     gene            complement(5287..5547)
                     /locus_tag="SMUNN2025_RS00535"
                     /old_locus_tag="SmuNN2025_0091"
     CDS             complement(5287..5547)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268321.1"
                     /locus_tag="SMUNN2025_RS00535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0091"
                     /product="hypothetical protein"
                     /protein_id="WP_002268321.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.9e-07,
                     bitscore: 27.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSLAFEKFESLDADCLVQFQGEGWVGAVAGGVAGFIGGAALGAE
                     AAAPLALIPGFGWVTDVGCATVGAISGAIGGAASGYKASAW"
     misc_feature    5637..5650
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.82"
                     /tool="antismash"
     misc_feature    complement(5721..5737)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.51"
                     /tool="antismash"
     misc_feature    complement(5754..5774)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 23.89"
                     /tool="antismash"
     misc_feature    5852..5865
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.93"
                     /tool="antismash"
     tRNA            6050..6135
                     /anticodon=(pos:107244..107246,aa:Leu,seq:aag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SMUNN2025_RS00540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SmuNN2025_tRNA0019"
                     /product="tRNA-Leu"
     gene            6050..6135
                     /locus_tag="SMUNN2025_RS00540"
                     /old_locus_tag="SmuNN2025_tRNA0019"
     gene            6292..7173
                     /locus_tag="SMUNN2025_RS00545"
                     /old_locus_tag="SmuNN2025_0092"
     CDS             6292..7173
                     /EC_number="4.1.2.13"
                     /GO_function="GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /locus_tag="SMUNN2025_RS00545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0092"
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="WP_002263577.1"
                     /transl_table=11
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAPV
                     LIQTSMGAAKYMGGYKLCKVLIETLVESMGITVPVAIHLDHGHYEDALECIEVGYTSIM
                     FDGSHLPVEENLEKAKEVVAKAHAKGISVEAEVGTIGGEEDGIIGRGELAPIDDAKAMV
                     ETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQIRAAI
                     KLGVAKVNVNTECQIAFANATRKFAAEYEANEAEYDKKKLFDPRKFLKPGFEAITEAVE
                     ERIDVFGSEGKA"
     gene            7497..7988
                     /locus_tag="SMUNN2025_RS00550"
                     /old_locus_tag="SmuNN2025_0093"
     CDS             7497..7988
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318003.1"
                     /locus_tag="SMUNN2025_RS00550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0093"
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_002272890.1"
                     /transl_table=11
                     /translation="MTVVEARVDQRLIHGIVVNQWNSKLNPKRFMIIDDIVSNQPEIKN
                     SMRMAKPSGKSMSIIDTQKAIANFKNGNYDKQDVFIIVKEPSTLLKLLDNGISIPRVNL
                     GIVFADDVRTNISKFVNLNQSEVNELKKIEERGIPIIIQYIPDDSVIVFEKAIENKKFK
                     "
     gene            8001..8798
                     /locus_tag="SMUNN2025_RS00555"
                     /old_locus_tag="SmuNN2025_0094"
     CDS             8001..8798
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /GO_process="GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351780.1"
                     /locus_tag="SMUNN2025_RS00555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0094"
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="WP_002265166.1"
                     /transl_table=11
                     /translation="MSIEFIQNILVILLSAYVVMDNLGITIFNYWAVTTGMLVGLIMGD
                     INTGLLIGGTFQLMSLGVAGLGGASVPDYGLAALVGTFLAIRTGSGLSTAVAVGLPVGL
                     LAINFDVLVKILNNFVAHKMQRLAHEGKYKQMLLWGWIGPIMFMLKSVIIVTIIVTVGP
                     GVIKSILNIIPQWVTDGLNIAGGMLPVLGIALLLHYMPAKKYLWAVLIGFVLSTYLKVP
                     IIGVSIVGAAAAILIYQSKAEEIVTSKEKMSSLSITDEGDYDE"
     gene            8788..9609
                     /locus_tag="SMUNN2025_RS00560"
                     /old_locus_tag="SmuNN2025_0095"
     CDS             8788..9609
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074476.1"
                     /locus_tag="SMUNN2025_RS00560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0095"
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="WP_011074476.1"
                     /transl_table=11
                     /translation="MMNKKEINNIITKRDRHNAILRWMFMGSALYNYETGQASSVVWSL
                     AKLLRKIYPNDEEYKEALDNHFNYFNTTTAMANIILGATTAMEEKDGIQTKDAVKSLKT
                     SLMGPFAGIGDTIIWVLLPTIMGSISGYMAVKGNPLGTIAWIIIGALLFWIRIKLFDIG
                     YTSGVKLVTNFSDKLSLFTEAMSAFGLMVVGTLVSTVVKVYTPLTFKAGKVTLLVQEKI
                     LDAIMPSLLAVVLTYVVYKMLESKWWTPTKIIMAIIIVSLLGAYTGILGVK"
     gene            9609..10022
                     /locus_tag="SMUNN2025_RS00565"
                     /old_locus_tag="SmuNN2025_0096"
     CDS             9609..10022
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290461.1"
                     /locus_tag="SMUNN2025_RS00565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmuNN2025_0096"
                     /product="PTS system, IIA component"
                     /protein_id="WP_002263581.1"
                     /transl_table=11
                     /translation="MERRIIIASHNHMASGLKSTIEFLAGIQNNIVTLDAYTDGKPIGD
                     KIEKIFNDFPQECEVVIFTDLLSGSVNQKFFPYRVREHVHLITGMNLPIILAMVLNHQE
                     VYLEEEQVSYMVQEARSSLVYVNEMNLNDEGDE"
ORIGIN
        1 tcactcacgc cgaaaaccct gaaaaaaact ctctatgata tcaatgatat tgaatcaggt
       61 tgtggctttg atttagacaa atttctaatc gctaatagct cggatgactt tctgacacta
      121 gtgattgata ttattgaaga gcataacttc ttggaattta acagtttagt ttattatgca
      181 cggacagaaa acccaaaatt actgagctta atcatggata agacttactt tttcgctaaa
      241 tatttagatt caagaagata tgataaaaat gaaaggaagc gtagacatgg caaatcttga
      301 actcagtaaa ttatcgcaaa tagaacaatt actaaattgc ttattggcat tagcaaaaca
      361 aaaccaaaac ggacaaaact taaccattgt tactcaaaag gaactactaa atatccttca
      421 aatctctcca aataccttga aatcatggga gagaacagga cttaacagac ttgaacctcc
      481 catagaaagg acaagaacag tctattaaca atatttatgc taaaataaga ctatcacatt
      541 aaatatgtga aggtgttatt ttagcctttt gaatatccaa atcaaagaag gctatatgca
      601 aaaagaaatt atttttcata acaataaaaa agttactaaa actactaaga ataacggtac
      661 tattagttac acccaaagag gtgtttattt aggtatagac atcaaaactg gcaagaaagt
      721 cacgtcatta attatcgcaa aaaccttacg aagtcttgac cgaaaaatca ctcaagcaaa
      781 attagatttt gaagcaagag gctcaactct aaaagaaact gtagctatta acacttttga
      841 agaattggct gagcatggtt ttctagtttt atcacgtggg tgtcatcaca aaatacgatt
      901 aatcgtgtta gaaattacct tgacacctac attattccta aatttggtga ctataaacca
      961 gaagaaataa aatcagctga tattcaaatt tgggtaaatg aactaagcaa gaaaatcaaa
     1021 attatctgtt gaatctggcg taaaaaaagg ctatgctaaa gattttggag ctgttttaca
     1081 caaattaaat taagtgatat ttttgatttt ggtatcacta attttgaatt agccaataat
     1141 cccgcacgga cagtcaaaat cccacctaag tcgaaagcca ataaagagcg cattatggta
     1201 cttcatgaag atgaacttgc tgcttggctg acctatctaa acagcttacc taatacaaga
     1261 gctaatagaa gatttaaggt tatctgcgat actctgcttg cttctgctct gcgaattaat
     1321 gaactgttag ctttgactat tcaggacctg gattttgaag ctaatgaaat ccttgttaat
     1381 aaaacactga tgtggaaggc agggaacaaa aaacttggta tcaagggcac gatggtttgt
     1441 aaaaatagtg ctaaaacaga ctcaggaaat cgtcgtgttg ctgttccaag acaaatttta
     1501 gaagatttgc aagcattcca caatgaaatg agttgctatt tcgagaagca taaccttcct
     1561 accgttgatt tgattttccc cactatctac gggaactata tgtgcgacag aaacgagcgt
     1621 gcaacactta aaaaacgcct aactaagtta ggattgccaa actatggttt ccacctcttt
     1681 cgccacaccc atgctccttt aatgttgaat gctggagcta attggaaaga acttcaaaca
     1741 agaatggggc ataaatctat taagacaaca atggatacct atgccgaatt agcccctaaa
     1801 aagaaaactg aagccgttga catttatctc aaacaaatag ctaagtttac tggctaatca
     1861 tatgactaca tttttgacta catttaatat tgtaaaatct ataaaccttg atataacagc
     1921 gtttatggtg tgctaaaata acatttccga acctttactg atctaatgta tttaaataat
     1981 ataataaaaa gcagcgttca tatcttgttt tgtgtttaaa aacctatatt tcaaaaaaat
     2041 attttatttg taaaatattc atacttagtt ttttatttcg aacggctttt tgcattattg
     2101 ttacaaagaa agactggtgg gcattatgcg taatttgtga tagttgattt gatgagattt
     2161 actatcttag gattttaaat aaagatactt cccttttatc agttttgaaa gaagaattaa
     2221 cctttgacga ataatacatt ttagcgtaaa atagtaagga acgataatta tagataatcg
     2281 gattattgtg gggatttagc cttgaccgat tatgataagg agaaccactt tggaattaga
     2341 agtatttgct ggacaagaaa aaagtgaatt gtcaatgatt gaagttgctc gtgccatttt
     2401 agaagaacgt ggccgagata aagaaatgta ttttagtgat ctcgttaatg cgattcaagt
     2461 ttatttagaa aagtcagatg cagacattcg tgaagcgtta ccatttttct attcagattt
     2521 aaacacagat ggcagcttta tcccgcttgg tgagaataaa tggggtctgc gctcttggta
     2581 tggtattgat gaaattgatg aagagattgt gacacttgaa gaagatgaag acggtgcacc
     2641 aaaacataag aggaaacgtg ttaatgcctt tatggatggt gatgaggatg ctattgatta
     2701 cagtgatgac gatcctgaag atgaaagttt taataccgct gaagagaata cagaatatga
     2761 tgaggaagat ccagatgatg aaaagtccga ggttgaatcc tatgattctg aaatcaatga
     2821 aatcattcca gatgaagatt tagacgaaaa tgttgattta gatgaagaag atgacgatta
     2881 ttctgatgat gaggaagatg aagaaggaga ataaatttct tcattgacaa aattcataag
     2941 atagtttata ttatttattg ggcacctctt ttgaggtcag aataagctct ctacttgtag
     3001 ggggcatttt ttcattttcc tcacaatata aaacaaagga gcatttaatg acaaaatata
     3061 tttttgtaac aggtggtgtc gtttcgtcaa ttggtaaggg aattgttgct gcaagtcttg
     3121 gacgtctttt gaaaaatcgt gggcttaaag taaccattca aaaatttgat ccgtatatta
     3181 atattgatcc tggaacaatg agtccttatc agcatggtga agtttatgtg actgatgatg
     3241 gtgctgaaac tgaccttgac cttggacatt acgaacgttt cattgatatt aatctgaata
     3301 aatattcaaa tgttacaaca ggaaagatat atagcgaggt tctgcgtaaa gaacgtaaag
     3361 gtgaatatct tggagcaact gttcaagtta ttccccatat tacagatgct ctaaaagaaa
     3421 aaattaaacg tgcagctaca acaacagact cagatgttat tatcactgaa gttggtggta
     3481 cggttggtga tattgaaagc ttgcccttcc ttgaagccct tcgtcaaatg aaagctgatg
     3541 tgggtgctga taatgtcatg tatattcata cgactttgct tccttatctc aaagctgcag
     3601 gagaaatgaa aacaaaacca acccaacact ctgttaaaga gctgcgtggt ttgggaattc
     3661 aacctaatat gcttgttatt cggacagaac agccagccgg acaaggtatt aaaaataaat
     3721 tagctcaatt ctgtgatgtt gctccagaag ctgttattga atcacttgat gtggttcatc
     3781 tttatcaaat tccgttaaat cttcaagcac aaaatatgga tcagattgtt tgcgatcact
     3841 taaaactgga tgtgcctgtt gccgatatga ccgaatggtc agcaatggtt gataaggtta
     3901 tgaatctcaa gaaaaagact aagattgctt tagttgggaa atatgtagaa ttgccagatg
     3961 cttatttatc agtcgttgaa gctcttaagc attcgggcta tgttaatgat acagccattg
     4021 atctcaattg gattaatgct aatgaggtca ctcctgaaac ggttactgat ttgctgggag
     4081 atgctgatgg tattattgtt ccgggtggat ttggccatcg tggaacagaa gggaaaatag
     4141 aagctattcg ttatgcgcgt gaaaacgatg tgcctatgct aggaatctgt cttggcatgc
     4201 aattgacttg tgttgaatat gctcgtcatg tccttaacct tgaaggagct aattctgcgg
     4261 aattggatcc agataccaaa tatccagtta ttgatattat gcgcgatcag attgatattg
     4321 aggatatggg aggaacgctt cgccttggcc tttatccatg taaattgaaa tcaggcagca
     4381 aaacagcatc agcttatagc aatcaagagg tcgtacagcg ccgtcaccgt catcgttatg
     4441 aatttaataa tgcctttcgt cagcaatttg aggaggctgg tttcgtattt tcaggagtat
     4501 caccagataa tcgcttggta gaagtggtgg aattgtcaga taaaaaattt tttgttgcag
     4561 ctcaatatca tccagaatta cagtctcgtc cgaatcgtcc ggaagaactt tatacagcct
     4621 ttattacggc agcggttgaa aatagtcagg cacattaaat tgatagaaaa atgagttact
     4681 tgacatagct ttcagtaaaa agaataaaga gattccatgt agattaaact tttgcttata
     4741 aagaaacacc ttcttaaatc tagtaaatga gatttaagaa ggtgtttctt cttttttgga
     4801 tcattattac agctaatcgt tcaattcaaa aatgaataaa tcatattagc tattacaaaa
     4861 atacattcca ggataaaaat tccaaaacag agaagcttga cagtaccgct tcggctatac
     4921 ttagaaatta agactaatac tgcgaaactt aatagtatga acgagtaaat aataaacatc
     4981 atatattcag ctcaatctct ttataatggc gtattgtaac cgaccccggc accaagaaca
     5041 gcaccagcaa tagtactggt agcaacaact ccggcttcag tagcccaacc aaagccaggt
     5101 atgcacataa ctggaagcgc agcagctcca ccagctttag caccggcaag agctccagtg
     5161 acacctccag caacagcacc gagccaacct tcaccttgga cttgtgcaag atagtcatca
     5221 tctaaagttt caaattgttc caatactaaa gtgttcataa aaatttctcc tttatctttt
     5281 aactttttac catgcggatg ctttataacc agaagcggct ccaccaatag caccactaat
     5341 agctccaaca gtagcacatc caacatcggt aacccaacca aaaccaggaa taagtgcaag
     5401 tggagcagcc gcttctgcgc ctaatgcagc acctccgata aatcctgcga cacctccagc
     5461 gactgcacca acccaaccct caccttggaa ttgaacaaga caatcagcat ccaaagattc
     5521 aaacttttca aaagctaaac tattcatact gaatctcctt taaataaata tggtttctat
     5581 acccgttata gttaaagaaa ttggctgcca acaaaacgaa tacacgtata gtttagtttt
     5641 tttaagaaaa attcaagagg attctatcat tgtaatgaaa tatttattat ttagacatta
     5701 ttatgttatt ttttctggtt ttttaagttt tttgcaaggc gaaattattt aattaaaagt
     5761 taccatgacc ataatcgaga aatgcaatta ttttttagta gctattattg tattaaatag
     5821 cttgaagtaa taacgaattt gattgtatta agtcactttg agtaatgagg tgattactat
     5881 aatttcaaag gaaaaatgtt gacgttttat agatatattt ttcagtatca aaagttttgg
     5941 ttttactttg gtggtttcaa ttgacctttt taagatttga cacttgaaaa aagcaaaaaa
     6001 actcttgaca ctatctttgt tagttgatat aatttaaaaa gttgctatcg cggggatggc
     6061 ggaattggca gacgcgcaag actaaggatc ttgtgaccgc ttttggtcgt gagggttcaa
     6121 gtccctctct ccgcaaagtg agctgactgt tcaagtcagc tttttttgtt gtctaggtca
     6181 gttagagaaa aggttgaaac ttttgataag ggctggcaat tttactgtaa aagtgataaa
     6241 ataaacaacg taatgggttt gacccaaaaa atatttagga ggcctttaaa aatggcaatc
     6301 gtttcagcag aaaaatttgt ccaagcagct cgtgataatg gttatgctgt cggtggattt
     6361 aacactaaca accttgaatg gactcaagct atcttgcgtg cagcagaagc taaaaaagct
     6421 cctgttctca ttcaaacttc tatgggtgct gccaaatata tgggtggtta taaactttgt
     6481 aaagttctta tcgaaactct tgtagaatca atgggaatca ctgtaccagt tgctatccac
     6541 cttgaccatg gtcattatga agatgctctt gaatgtatcg aagttggcta tacttcaatt
     6601 atgtttgatg gttctcatct tccagttgaa gaaaaccttg aaaaagctaa agaagttgtt
     6661 gcaaaagcac atgctaaagg tatttcagta gaagctgaag ttggtactat cggcggtgaa
     6721 gaagatggta tcatcggccg cggtgaattg gcaccaattg atgacgctaa agcaatggtc
     6781 gaaaccggta ttgatttctt ggcagctggt attggtaata ttcacggtcc gtacccagca
     6841 aattgggaag gtcttgacct tgatcacttg aaaaaattga cagaggctgt tccaggtttc
     6901 ccaattgtac ttcacggtgg ttcaggtatt cctgatgatc aaatccgcgc agcaatcaaa
     6961 cttggtgttg ccaaagttaa cgttaatact gaatgtcaaa tcgcatttgc taatgctaca
     7021 cgtaaatttg ctgctgaata cgaagcaaat gaagcagaat acgacaagaa aaaactcttt
     7081 gacccacgta aattcttgaa accaggtttt gaagctatta cagaagcggt tgaagaacgt
     7141 attgatgtct ttggttcaga aggtaaagct taattacttg gttaacaaat tgaaagattc
     7201 tctttcccta gactgaggaa atcctatttt ggataatttc ttcagtcttt ttgttttctc
     7261 ttttgaggga ggcacaagat taaaattaga attaatgatg caccatggat aaacgaaaaa
     7321 gtatagtaaa aattaaggct tgaaatctag aaactacaaa aatcattata gaaaatcaga
     7381 aagggttcct tgacaatatg gaacgttcca tgtataatat aatcactaaa acgcttacac
     7441 ttaaagtagt tttttgagag aaattgaacg ttcaatattt taaggaggaa tttattatga
     7501 cagttgttga ggctagagta gatcaaagat taatccatgg aattgtagtg aatcagtgga
     7561 attcgaaact gaatcctaaa cggtttatga ttattgatga tattgtaagc aatcagccag
     7621 agataaaaaa ttctatgagg atggcgaagc cctctgggaa aagcatgagt attattgata
     7681 cacaaaaggc tattgcaaat ttcaaaaatg gtaattatga taagcaagat gtgtttatca
     7741 ttgtcaaaga accttctact ttgcttaaat tacttgacaa tggtatttct attcccagag
     7801 taaacttagg aattgtgttt gctgatgatg ttaggacaaa tatcagtaag ttcgtaaatc
     7861 tgaatcaatc tgaagtaaat gaattgaaga aaattgagga acgtggaatt ccaatcatca
     7921 ttcaatatat tccggatgat tctgtaattg tctttgaaaa ggcaatagaa aataaaaaat
     7981 ttaaatgaga gggaacaaaa atgtcaatag agtttattca aaatatttta gttattttat
     8041 tgtcagccta cgtggtaatg gataatcttg gaattacaat ttttaattat tgggctgtaa
     8101 caacaggcat gcttgttggt ttaataatgg gggatatcaa tacaggactt ttaattggag
     8161 ggacttttca attaatgtct ttaggtgttg ctgggctagg cggagcttcc gttccggatt
     8221 atggacttgc tgctcttgtt ggtacgtttt tggcaattag gacaggatcg ggtttgtcta
     8281 ctgcggtagc agttggctta ccagtaggtt tattagcgat taattttgat gttttagtaa
     8341 aaattttaaa taattttgtg gcacataaaa tgcaaagatt agctcatgaa ggcaaatata
     8401 aacaaatgtt attatggggt tggatcggac caataatgtt tatgttaaaa agtgttatta
     8461 ttgtcactat tattgtgaca gtaggtccag gagttatcaa atccatttta aatattattc
     8521 cacaatgggt tacagatggt ttaaatattg ctggtggaat gttaccggta ttggggattg
     8581 cactgttatt acactatatg cctgcaaaga aatacttatg ggcagtttta ataggatttg
     8641 tacttagtac ctacttaaaa gttccaatca ttggggtctc aattgttggt gctgcagctg
     8701 caattttgat ttatcaaagt aaagctgaag aaattgttac ttcaaaagaa aaaatgagct
     8761 ctttaagcat tactgatgaa ggagattatg atgaataaaa aagaaattaa taatattatt
     8821 acaaaacgtg atcgtcacaa tgctattctc agatggatgt tcatgggttc agcattatat
     8881 aactatgaaa cagggcaagc aagctcagtt gtttggtcct tagctaaatt gctaagaaaa
     8941 atatatccaa atgatgagga atacaaagaa gcattggata atcattttaa ttattttaat
     9001 acaacgactg caatggcaaa tattatactt ggggcaacaa ctgccatgga agaaaaggat
     9061 ggaattcaaa ctaaggacgc agttaaatca ttaaaaactt ctcttatggg accttttgca
     9121 ggaattgggg acaccattat ctgggtttta ttaccaacca tcatgggttc tatttcgggc
     9181 tatatggcag ttaaaggcaa tcctttaggt acgattgcat ggattattat tggggccctt
     9241 ttattttgga tacgtattaa attatttgat attggttata catcaggtgt gaagttagtc
     9301 actaatttca gtgataagtt gtctcttttt acagaagcca tgtcagcttt tggtctgatg
     9361 gttgttggaa cattggtatc tacagtcgta aaagtatata cgcctttaac tttcaaggct
     9421 gggaaggtaa ccttgttagt gcaagagaaa attttagatg ctattatgcc ttccctactt
     9481 gcagtggtgt tgacatatgt tgtttacaaa atgcttgagt ctaaatggtg gacgcctact
     9541 aaaataatta tggcaattat tattgtttca ttgctaggag catatacagg aattttaggt
     9601 gtaaaatgat ggaaagaaga atcatcatag cttctcataa tcatatggca tcaggtctaa
     9661 aaagtaccat agaatttcta gctggtatcc agaataatat agtaacatta gatgcctata
     9721 ctgatgggaa accgattgga gataaaatcg agaaaatttt taatgatttt ccccaagaat
     9781 gtgaagtcgt tatttttact gatttactct cgggatctgt taatcaaaaa ttttttccat
     9841 atagagttag ggaacatgta catctgataa caggaatgaa cttacctatt attttagcca
     9901 tggttttgaa tcatcaggaa gtatatctgg aagaagaaca ggtaagttat atggttcagg
     9961 aggcgcgttc atcattggtg tatgtaaatg aaatgaattt aaatgatgag ggggatgagt
    10021 gatggaaaaa ataattagag aaggttcagt acgcttttca attattacaa attcacttct
    10081 aagaattgag gaagatattg aaaaagtatt tgaagatcgt ccttcaacaa cagtactaaa
    10141 tcgttccttt ggagaagtta atgcggagat aatccgaaat cataataatc atatcttgga
    10201 aatagtgact ccctcgtttc acttgtatta tgatggtggc tctctatctc caatatcatt
    10261 atacgtagat attaaaaagt ccagttctct ttatcataat agatggtttt ttggtaagaa
    10321 aaatgggaca aaaaatctta aggggactgt cagaactttg gatcgtgccg atggtgaagt
    10381 tcctctggaa gacggtctta tgtctaaaga tggttttagt tctttagatg attcaacaaa
    10441 ttttttatat ataaaagact cagattcatt tgtggcaaga agaaagggaa cggtagattt
    10501 atacctattt tgctatggga gggattatca agatgagtta actgatt
//
