LOCUS       NC_011900              10297 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Streptococcus pneumoniae ATCC 700669, complete sequence.
ACCESSION   NC_011900
VERSION     NC_011900.1
KEYWORDS    RefSeq; complete genome.
SOURCE      Streptococcus pneumoniae ATCC 700669
  ORGANISM  Streptococcus pneumoniae ATCC 700669
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Croucher,N.J., Walker,D., Romero,P., Lennard,N., Paterson,G.K.,
            Bason,N.C., Mitchell,A.M., Quail,M.A., Andrew,P.W., Parkhill,J.,
            Bentley,S.D. and Mitchell,T.J.
  TITLE     Role of conjugative elements in the evolution of the
            multidrug-resistant pandemic clone Streptococcus pneumoniaeSpain23F
            ST81
  JOURNAL   J Bacteriol 191 (5), 1480-1489 (2009)
   PUBMED   19114491
REFERENCE   2  (bases 1 to 2221315)
  AUTHORS   Croucher,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2008) Croucher N.J., Pathogen Genomics, Wellcome
            Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton,
            Cambridge, Cambridgeshire, CB10 1SA, UNITED KINGDOM
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 00:47:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,195
            Genes (coding)                    :: 2,039
            CDSs (with protein)               :: 2,039
            Genes (RNA)                       :: 73
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 82 of 156
            Pseudo Genes (incomplete)         :: 70 of 156
            Pseudo Genes (internal stop)      :: 58 of 156
            Pseudo Genes (multiple problems)  :: 45 of 156
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:23:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1945305
            Orig. end                         :: 1955602
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FM211187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(67..945)
                     /gene="rsgA"
                     /locus_tag="SPN23F_RS10220"
                     /old_locus_tag="SPN23F20040"
                     /old_locus_tag="SPN23F_20040"
     CDS             complement(67..945)
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0043022 - ribosome binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsgA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /locus_tag="SPN23F_RS10220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20040"
                     /old_locus_tag="SPN23F_20040"
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="WP_001161981.1"
                     /transl_table=11
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVDF
                     SAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHKGI
                     HPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGVGKS
                     TLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVSRAED
                     LNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEIENRRE
                     TYKKVSKKIPK"
     gene            complement(947..1819)
                     /gene="rsmA"
                     /locus_tag="SPN23F_RS10225"
                     /old_locus_tag="SPN23F20050"
                     /old_locus_tag="SPN23F_20050"
     CDS             complement(947..1819)
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="SPN23F_RS10225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20050"
                     /old_locus_tag="SPN23F_20050"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_001216856.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
     gene            complement(1950..2258)
                     /locus_tag="SPN23F_RS10230"
                     /old_locus_tag="SPN23F20060"
                     /old_locus_tag="SPN23F_20060"
     CDS             complement(1950..2258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /locus_tag="SPN23F_RS10230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20060"
                     /old_locus_tag="SPN23F_20060"
                     /product="hypothetical protein"
                     /protein_id="WP_000333253.1"
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYLL
                     DFASIEGQRQKHYRLKNLPQMVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC"
     gene            complement(2251..2892)
                     /locus_tag="SPN23F_RS10235"
                     /old_locus_tag="SPN23F20070"
                     /old_locus_tag="SPN23F_20070"
     CDS             complement(2251..2892)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="SPN23F_RS10235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20070"
                     /old_locus_tag="SPN23F_20070"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565531.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(2894..4924)
                     /locus_tag="SPN23F_RS10240"
                     /old_locus_tag="SPN23F20080"
                     /old_locus_tag="SPN23F_20080"
     CDS             complement(2894..4924)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="SPN23F_RS10240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20080"
                     /old_locus_tag="SPN23F_20080"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000825254.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMINQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGKLPLKR
                     IMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(5001..5297)
                     /locus_tag="SPN23F_RS10245"
                     /old_locus_tag="SPN23F20090"
                     /old_locus_tag="SPN23F_20090"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="SPN23F_RS10245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20090"
                     /old_locus_tag="SPN23F_20090"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815392.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 6.4e-20,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     misc_feature    complement(5447..5460)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     misc_feature    complement(5537..5546)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(5617..6516)
                     /locus_tag="SPN23F_RS10250"
                     /old_locus_tag="SPN23F20100"
                     /old_locus_tag="SPN23F_20100"
     CDS             complement(5617..6516)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /locus_tag="SPN23F_RS10250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20100"
                     /old_locus_tag="SPN23F_20100"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000904834.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEIYK
                     TYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLLIRL
                     FFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVGVTDC
                     FNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGDG
                     FLVKKLTEEWQEDVKKYL"
     gene            complement(6588..7148)
                     /gene="rnmV"
                     /locus_tag="SPN23F_RS10255"
                     /old_locus_tag="SPN23F20110"
                     /old_locus_tag="SPN23F_20110"
     CDS             complement(6588..7148)
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="SPN23F_RS10255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20110"
                     /old_locus_tag="SPN23F_20110"
                     /product="ribonuclease M5"
                     /protein_id="WP_000660009.1"
                     /transl_table=11
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(7148..7921)
                     /locus_tag="SPN23F_RS10260"
                     /old_locus_tag="SPN23F20120"
                     /old_locus_tag="SPN23F_20120"
     CDS             complement(7148..7921)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="SPN23F_RS10260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20120"
                     /old_locus_tag="SPN23F_20120"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575597.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIER
                     ALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVETTANAERIFGLDSK"
     gene            complement(8226..9119)
                     /locus_tag="SPN23F_RS10265"
                     /old_locus_tag="SPN23F20130"
                     /old_locus_tag="SPN23F_20130"
     CDS             complement(8226..9119)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /locus_tag="SPN23F_RS10265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20130"
                     /old_locus_tag="SPN23F_20130"
                     /product="DUF1542 domain-containing protein"
                     /protein_id="WP_000226481.1"
                     /transl_table=11
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKVA
                     ILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEYKL
                     ENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIE
                     KAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIA
                     TVTVPKRLVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVV
                     ATSGFALLGRKTRRRK"
     gene            complement(9403..9537)
                     /gene="rpmH"
                     /locus_tag="SPN23F_RS10270"
                     /old_locus_tag="SPN23F20140"
                     /old_locus_tag="SPN23F_20140"
     CDS             complement(9403..9537)
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="SPN23F_RS10270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F20140"
                     /old_locus_tag="SPN23F_20140"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
ORIGIN
        1 tcaaagttgg cataatctgc tgccagaatt gacggagcaa tcttgtattg agacataggt
       61 ttctccttat tttggaattt ttttgctgac ttttttatag gtttctctac gattttcaat
      121 ttcactaagg aattgcaggt aattgtcaaa acggaaggtt gcaataacac cctcttcaac
      181 agctggtttg acggcacaag acggctcatg ggtatgggta caagtacgga acttacaatc
      241 tcggctaaca gtagcaatct ctgggaaagc ctgattgagg tcttcagccc ttgatacttc
      301 atagtccaag gatgaaaatc ctggtgtatc tgcgatttta cccccgttga gattgtaaaa
      361 actaacagct cgagtggtat ggcgaccgcg acctagactg tctgaaattt ctcccgtttc
      421 aagattgagg tctggtgcga ttttattgag aagagttgac ttcccaacac ctgtctgccc
      481 cataaagacc gtaaccttgc ctgttaacaa agacaggagt tcctctttac tggtcacaaa
      541 gtcatagccg atgtcaccat aggtctgctg gtaaaaatcc agttctcccc tatcttccaa
      601 caaatccatt ttggaaatat agacaatggg atggatgccc ttgtgctcca aaagaaccaa
      661 gaaacgatcc agcaaattgc tgttaaaatc aggttccttg acggacatga ttactacagc
      721 ttgatcgata ttgacaatag gcggacgaac cagactgttt ttccgttcgt gaattttgag
      781 gatatagcct tctgaatttt cctcggcaga gaaatctacc cagtccccaa cataaggggt
      841 atggcctttt ttacggaaat tcccacgcgc gcgtgtttga taaacctggc catcactctc
      901 cacatagtag aaacctgcca aggctttaat gatttgtccc tgcatcttag agtccttgcc
      961 ctttaagtgc gtctgctaga ccggcaaatt ctgccaagct gagagcttcc ccacgcacac
     1021 ttggtgacaa gcctgcctgg tccaaagcct tggtcagctt gtccttgacc tcttcagtct
     1081 taccaaagta acctgtcaag ttattccaca aggtcttgcg gcgatgggta aaactagcct
     1141 tggaaacctt aaagaaaaag ttctcatctt ctactgctac ggctggctct ggacgacgca
     1201 ccattttcaa gatggctgaa tccacatttg gcgctggcac aaagaccgta cgaggcacga
     1261 taaaggcaac cttggctgtc atgtaatact gcacggcgat agacaagcta ccgtaagcct
     1321 tggtgttagg ctgggctgaa atgcggtccg ctacttcttt ctgcatcatg accacaaact
     1381 cacaaaaagg aatgccactc tcaatcaagt gcatgagaat aggcgtcgtg atgtagtaag
     1441 gcaaattagc cactaccttg attggcaggt caggattttt aaaattctgg atatgttgcg
     1501 ccaaatcaac cttgagaata tcttcgttaa ctacggtcac attatcaaaa tcacgcaggg
     1561 tatctgccaa aattggcacc aaacggtggt caatctcaaa agccatgact tgggctgcac
     1621 gctcagccaa aaattctgtc aaggcaccaa tacctggccc gatttcgatg acattgacct
     1681 gatcatcaat ttcagccgta tccacaattt tttgaaggat attggtatcc gtcaaaaaat
     1741 tttgcccaaa ggacttttta aaggtaaaac cgtgacgctc cagcactgcc ttggtcacgc
     1801 tataatctgc aattctcatc tattctcttt tctattatct gttactacta ttgtaacaca
     1861 ttccccttgt attttgggaa tgaaaatcat accaaaaaac tatctttcct catgaagaaa
     1921 gatagtcttg gaaatcttta aaagtggcct tagcagttct ctcgaaacat gcgttcaaaa
     1981 aaatcagctt cttgataact tcgattttca ggtagggtca aatccacatc ctcctccaca
     2041 tcatccactg tcagttcaac catctgaggt aaatttttca aacgataatg cttttgcctt
     2101 tgcccttcaa tactagcaaa atcaagcaaa tagccatgca ctcggatttg aaccaaaagg
     2161 agatgattgg tcatattttc catgatgagg atatcttcta aaccacctct ggcactagga
     2221 taatcagata ccctgcgaat acggattttt tcacacatga gcaagtttcc tcatatcaat
     2281 gatttcatca gccttattcc agactagggg attatgggtc gcaatgatga taattcgatt
     2341 ttcatctttc aaatccacca agagattcat aacctcctct gaattttcag gatcaagagc
     2401 tgctgttggt tcatctgcca aaatcaaggg tggatttttc aaaatagtct tagcaagggc
     2461 aactcgttgg gcctctcccc cagataaagt atagattttt tgttctaaat ccaaataccc
     2521 tagattaact ttttctaaag cccccacttg cctttccaaa cgttctactt ttgagatttt
     2581 ctgaccaaca aaacccaaat ccaaattttc tttgattgat tggttttcta agaggccgaa
     2641 attttgaaag agatagccca tctggtctcg aaaatactca cgagtgggaa tggtttttaa
     2701 atctttcccc tgatagagaa cccttccacc atctatcttt tctagctttc ccaagatatt
     2761 cagcagcgtc gtctttccgc ttccactctt tccgattaag gcataaacct tgcccttctc
     2821 cagcttgaga ttcaaaccag agaaaatcgc gcggtcatta aatttctttt ccaatccttg
     2881 aatatcaatc atcttatttt cctttcaata ctgccatgct accagcttct tcttttttgt
     2941 cctgccttac taaaatcaag agggcgttaa gcgtaaacaa agctacaact agagcgctaa
     3001 tcaaaccatc tcttgtcaaa atactagata ggactaggcc aagcaagaga actcctcctt
     3061 gcgccagtaa atacttgcca tgaagctcat agattgtcat accagcaaga cgtttaatca
     3121 taagttcccg tctgaactgc tcaaaataga gaagattcat ggaatcaaat aacaagatag
     3181 ccgtagacag ggtcaaaatc gtcccaataa ggagagagta aaactccacc cgtttgtcat
     3241 ttagtacctt ggcaaaaaat agctggcttt ttaccaagta agaaaccttg tgatacagac
     3301 ctttctcttt tagagctgtg atgctgtctc catatcgatc tagtttcaag gcactattag
     3361 ccgtagttcc ccacaacatc cctgcaacag gtcttgttcc agtcgcttgc ggcgttagaa
     3421 ctacaataat cggatctttt aggtactggc gtgttgtctt gtacccatca ttatagagga
     3481 aacgttcctg tcctgtttct gtaaaagcta gccttacctg tgggaggtag tgtttctgac
     3541 tcgtcacttc tacactttca gatgaaaagt tttgcaggta atctgtaaac aatccttggt
     3601 agtagacaga ctgctctcgc aagctctcag gcaagatcag cccaaactct ccctcagaca
     3661 agttttgcat cttgtccata aactctgaag aaacggtaat cttttcttct atcagatagc
     3721 gtggtgagac atagataaca ttccctgacg gtgtgtagtc actgagacga ttgccatcta
     3781 ggtccacttc tgctccatct gagaaattgt caacattgct cataatataa aaagagtctg
     3841 tattggctaa ccgttcttca gtaaatgtct gccactcacg attatcctta cgcgttcctt
     3901 cttcatcggc aaatgcacta gaccaaccaa aggatagacg gtaacggtct gaggactggc
     3961 tccatttatt gctagctctc tccatttcct gcatttcacg gtagtggggt aggagagctg
     4021 tcgcactcga tccgaccacc aatacagcta agagttgccc caccatcatc aatgtcatta
     4081 tacgtttgag agggagtttc cctttcaata gatccaccag actattttcc tgtaaaccaa
     4141 gtagatagac gacacttagt aaggtagaaa tccctgccag ggtcaatcca taaagtagaa
     4201 gagcaatgat gaccagttgc accgttgcca taaacaaggc accttgatac cagagaatcc
     4261 ccaatcccaa aagactggat accagcactg agcagataag ctgtctcaca tcttctaaca
     4321 ctggtctgag agcaactcca aacaagctct caccagctat taagcgaatc cctgcctgac
     4381 gaagggattt gatccgataa atcagggtca gactcataaa ggtcagcaga aaaatagcta
     4441 aactcagtag caccataggg gtggccgtca atagtaagac tatcgaaaat ggatcttctc
     4501 cattcgaaac aaagccttga taaccaagct ctttcaaggt ggtctgtaag ctcactccat
     4561 ccaaacttcc tgatacaatc aagtaagacc ctaataaatc actcgtttct gcactctcct
     4621 tggaggaaat ggtcagacct tctggaagct ttccctcacc ataagtggca taggtaaagc
     4681 gtgtttctcc agcttcattt ggctcaacga ttcgacgagc aatgagacta ttgtgctcta
     4741 ccgccaagtg actcaaagcc tctgtcacct cttcgcgact agctactggt tgatttatca
     4801 tcattcctgt cacatcaaca gaaggatagt taccaaataa aatctcttct cgatggtcaa
     4861 cggaagttat caccatataa agcgatacta ataccattga aatcaaaata aataaacgtt
     4921 tcatcatttc ctccaaaaag ttacttggtc acttagtgag aaggtgacca agtaaagcta
     4981 aacaataggg ctataccatt ctaaacatta tagtagaatg caactttctc tccccaagaa
     5041 gtacgaatcc acgcacgaga agtccctcca gcaccagcat atcccttgtc agatgaacca
     5101 gtttttctgc taacaactgt tgaactatgg tattttgaag ggtgatagta gtttgagtaa
     5161 gcaccaccgt tacctgaacc ataagtccaa acaccacctt ctgggtattg aacggctgca
     5221 gaggcaactg atacacctcc acctaatgtt gctgcaatgc tcgctacaag agctaacttt
     5281 ttaactgttt gtttcatatt gatactcctc tatttttgat tacaagattg ttgagttact
     5341 tgctcctctt tccattatca acgttgatac tattatatta ccaaatgata ttctaaaatt
     5401 atttgttttt ctctaaattg tcaaaatagc accctgaaat gaacttttat agtcattata
     5461 ctccatcttt atcacactat caaggacatc aatgtcctta aaatctaact atttcatatt
     5521 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     5581 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     5641 ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct tcgcaaatgt
     5701 tttagaagac tgataaaatg attcagctaa actaaaatct ctttctactc ttaatgcttg
     5761 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     5821 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     5881 acacattcct gaaataagag tatctctaat tataaagtaa ttctctattt gagttacctt
     5941 cttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     6001 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttattgaata
     6061 agcttctttt tctataatct gaggcatatg ttcctcaagt attaactctg catattcagg
     6121 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     6181 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     6241 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     6301 agcagaagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     6361 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     6421 ttgacgaact gtcagttctt gctcatctcc acaaaaatct ggacgactaa ttcctttttc
     6481 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     6541 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     6601 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     6661 tgcttgccgt tggaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     6721 ctgtctgccc ctgctagaaa accaaggcga atcaagtcgc tacgactaat gtcaaactga
     6781 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     6841 gcatgctcaa ttcccagaga acgccccttg gtcttggact tgggaacagc ttcatctcgc
     6901 ttgagaaagg catgctgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     6961 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgtag acgctgaatt
     7021 cgctctatat cctgatcatt gatggcagaa cctcgtgtct cataggtctc cacatcgaaa
     7081 taacgcttga gattgaccgt atcatcgcga ccttcaacca cgataacttg agaaattttc
     7141 tctttcatta cttgctgtcc aatccaaaaa ttcgttctgc atttgcagtc gtttctaccg
     7201 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     7261 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     7321 ccaccaaaat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     7381 tgaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     7441 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     7501 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     7561 agtccttaga tagctgaatc tgacgacgaa aaacctgctc ctgcacctct ttgggcgctg
     7621 tcatccaatg gtagtctaag ccaatttcac ctagagccac aaccttggga tgttttaact
     7681 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     7741 caatagtcgc atagagctgc tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     7801 gtttatcaaa accaacaata ttcatctgta tcacacccat ctcagcagcc aaggcaattt
     7861 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     7921 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggagga gcacctaatt
     7981 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     8041 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     8101 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     8161 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     8221 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     8281 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     8341 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     8401 gttgtcttag gagcagcatt aggagccact agtctcttag gaactgtaac cgtggcaatg
     8461 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     8521 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     8581 tcagaaagcg gtgcgccttt gatttcatcc tgcttgttct tggctgcttt ttcaatggca
     8641 tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     8701 tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgcctgcatt cgcattagca
     8761 tctgagcttc cagtattgcc tcccttatct ccacctggtg tattttctag cttatactct
     8821 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     8881 agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc tccagctaca
     8941 ttgattgtcg ctccatctaa tgctgaacca tttgcttgta aaatagcaac cttaactttt
     9001 tcttcatctg ttaaatgagt tatatctttt acatctactc tttgagcagg tgctttaaca
     9061 gttgattttt cagcagtcgg tttagtgcta tcttgtgctt ttggatcttg tgctaccaat
     9121 tgatttcctg gaattgtaat agctgaacca tctttaaaga cgattgtcgc tgtgccatct
     9181 ccagctactt caactttata gtaaaatgaa ataataacag gacaaatcga tcaggacagt
     9241 caaatcgatt tctaacaatg ttttagaaat agagatgtac tattctagtt tcaatctact
     9301 ataacatcgc ttccgaatcc atttgcatct actgaacttt tttacaaaaa taaaaatcag
     9361 tagactcgag ttcctactga tttttgatag aattcgtttg gattatgcag ccaaaacttt
     9421 gcgtccttta cgacgacgag ctgccaatac gcgacgacca tttttagttg acatacggtt
     9481 acggaatccg tgtttgcgcg cacgacgaag tttacttggt tgataagtac gtttcacgat
     9541 gaatacctcc tcatagattt tgtattcgtt tagccggcta taaagtgatc agttactaaa
     9601 catactttac tattctatct gattctttca cttttgtcaa tagctctagg caaatgtttc
     9661 cagctttttc gaatgcaagc cctatctacg atactgtttc aagaaacgag tcatcttttc
     9721 ttggatttgg gctaactcat caacacgagg aaggtaaacg atacggaagt ggtctggttc
     9781 ctgccagtta aagcctcgac catgaaccaa gagaaccttt tcctgcttca agaaatcaag
     9841 gacaaactgc tcatcatcat cgatacggta catattgcgg tcgatttttg ggaagatata
     9901 gagccccgcc ttgggtttaa cggcagacaa acctggaata tcttgaatgg cattatagat
     9961 gaaatttctt tgctcgtaga ttcgtccacc aggaagaagc aattcatcga ctgattggtg
    10021 accccccaag gaagtttgta cgacttgttg ggccaaaacg ttagagcaaa ggcgcatatt
    10081 ggacagcata ttgagccctt cgatatagcc cttaacatga gtcttagggc cagacaagac
    10141 catccaaccc acacggaaac ctgctatgcg gtgggatttt gacagaccat tcatgctgac
    10201 acagaagaca tctggtgcca agctcgccac aggcgtatgc acatgtccgt ccattaccat
    10261 gcggtcatag atttcatccg caaagataat caaatcg
//
